Rh6DG076400

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0014154
Physical Location & Seq
Forward (+)
8696072 .. 8712546
16475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG076400.1

Sequence Viewer

Length: 573 bp
ATGCTCATCCCGACCCGACCCTCAAAAAGGACTTTCGAAGAAGAATTGAAGATGTTCAAGGTAGTGCTGAACGATCGTCTTGGGAAGAAGGTAAAGCTGAAGGACAACGAGAACGACATCATCAACTTCCTGGAAGCTAGAGCTCCTCTTCTGATTTCTTCTCCTTCTCAAAATTCCGGTGTGAGGATCATCTCCCACATCGATTACCATCACTTCCAGACCCTAAATCGAAGAGTAAACCCAATTGTGCTACCTGATTTGAATATTCGCCGTTTTAAGGTTGCGGCAAGCGGGGATGTCGACGGCGTGGTGGACAATGTTGTGGATGGTACTGGAGGTGGTGGGGACGACGGCGGCTTTGACTACAATTGGGATTGCAAGATAGTAGTGTATGAATGGACAGGAAAGTGTAGGAGTTGCCAGGGCTCAGGATACGCTAGTTATTATAATAAAAGAGGGAAGGAAAATGTCTGCAAATGCATACCTTGCCAAGGAATCGGATATGTACAAAAGATAACAACTCGCACCAACATTGATGTAATGGAAGATTTGGATAACGGAAAACCACCATGA

Protein Analysis

190

Amino Acids

21.31

Weight (kDa)

8.48

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19220
fragaria_vesca FvH4_2g06130 FvH4_2g06130
malus_domestica MD10G1094200.v1.1
prunus_persica Prupe.8G124000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0257061
rosa_laevigata RLG00000014674
rosa_multiflora Rmu_sc0000659.1_g000009 Rmu_sc0038017.1_g000001 Rmu_sc0038018.1_g000001
rosa_roxburghii Rroxscaffold_7G00208470
rosa_rugosa Rorug05G0575500
rosa_samantha Rh6AG093500 Rh6BG084300 Rh6CG081000 Rh6DG076400
rosa_wichuraiana Rw6G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
AccI GTMKAC 1 cut(s) 300
AciI CCGC 3 cut(s) 284, 291, 354
AclWI GGATC 1 cut(s) 194
AcsI RAATTY 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 119
AfaI GTAC 2 cut(s) 331, 507
AfiI CCNNNNNNNGG 4 cut(s) 27, 183, 277, 491
AgsI TTSAA 3 cut(s) 49, 58, 262
AjnI CCWGG 2 cut(s) 129, 420
AluBI AGCT 3 cut(s) 97, 137, 143
AluI AGCT 3 cut(s) 97, 137, 143
Alw21I GWGCWC 1 cut(s) 145
AlwI GGATC 1 cut(s) 194
ApoI RAATTY 1 cut(s) 172
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
Asp700I GAANNNNTTC 1 cut(s) 53
AsuII TTCGAA 1 cut(s) 36
BaeI ACNNNNGTAYC 2 cut(s) 424, 457
BanII GRGCYC 2 cut(s) 145, 428
Bbv12I GWGCWC 1 cut(s) 145
BccI CCATC 2 cut(s) 216, 320
BceAI ACGGC 3 cut(s) 255, 319, 367
BciT130I CCWGG 2 cut(s) 131, 422
BciVI GTATCC 1 cut(s) 425
BfaI CTAG 2 cut(s) 138, 438
BfuI GTATCC 1 cut(s) 425
BisI GCNGC 2 cut(s) 285, 355
BlsI GCNGC 2 cut(s) 286, 356
Bme1390I CCNGG 2 cut(s) 131, 422
BmrFI CCNGG 2 cut(s) 131, 422
BpmI CTGGAG 1 cut(s) 354
Bpu10I CCTNAGC 1 cut(s) 427
Bpu14I TTCGAA 1 cut(s) 36
Bsa29I ATCGAT 1 cut(s) 201
BsaBI GATNNNNATC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 421, 490
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 183, 277, 491
Bse1I ACTGG 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 207
BseBI CCWGG 2 cut(s) 131, 422
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 2 cut(s) 421, 490
BseGI GGATG 3 cut(s) 6, 301, 331
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 4 cut(s) 27, 183, 277, 491
BseMII CTCAG 1 cut(s) 441
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 135
Bsh1285I CGRYCG 1 cut(s) 76
BshVI ATCGAT 1 cut(s) 201
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 145
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 359
BslI CCNNNNNNNGG 4 cut(s) 27, 183, 277, 491
BsmFI GGGAC 1 cut(s) 359
Bsp119I TTCGAA 1 cut(s) 36
Bsp1286I GDGCHC 2 cut(s) 145, 428
Bsp1407I TGTACA 1 cut(s) 505
Bsp143I GATC 2 cut(s) 73, 186
BspACI CCGC 3 cut(s) 284, 291, 354
BspCNI CTCAG 1 cut(s) 440
BspDI ATCGAT 1 cut(s) 201
BspPI GGATC 1 cut(s) 194
BspT104I TTCGAA 1 cut(s) 36
BsrGI TGTACA 1 cut(s) 505
BsrI ACTGG 1 cut(s) 337
BssECI CCNNGG 2 cut(s) 421, 490
BssMI GATC 2 cut(s) 73, 186
BssT1I CCWWGG 1 cut(s) 490
Bst2UI CCWGG 2 cut(s) 131, 422
Bst6I CTCTTC 2 cut(s) 153, 226
BstAPI GCANNNNNTGC 1 cut(s) 486
BstAUI TGTACA 1 cut(s) 505
BstBI TTCGAA 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 1 cut(s) 427
BstENI CCTNNNNNAGG 2 cut(s) 25, 489
BstF5I GGATG 3 cut(s) 6, 301, 331
BstKTI GATC 2 cut(s) 76, 189
BstMBI GATC 2 cut(s) 73, 186
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 1 cut(s) 486
BstNI CCWGG 2 cut(s) 131, 422
BstSCI CCNGG 2 cut(s) 129, 420
Bsu15I ATCGAT 1 cut(s) 201
BsuI GTATCC 1 cut(s) 425
BsuTUI ATCGAT 1 cut(s) 201
BtsCI GGATG 3 cut(s) 6, 301, 331
Cac8I GCNNGC 1 cut(s) 289
ClaI ATCGAT 1 cut(s) 201
Csp6I GTAC 2 cut(s) 330, 506
CviAII CATG 1 cut(s) 570
CviJI RGCY 5 cut(s) 97, 137, 143, 357, 426
CviKI_1 RGCY 5 cut(s) 97, 137, 143, 357, 426
CviQI GTAC 2 cut(s) 330, 506
DdeI CTNAG 1 cut(s) 427
DpnI GATC 2 cut(s) 75, 188
DpnII GATC 2 cut(s) 73, 186
Eam1104I CTCTTC 2 cut(s) 153, 226
EarI CTCTTC 2 cut(s) 153, 226
Ecl136II GAGCTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 490
Eco24I GRGCYC 2 cut(s) 145, 428
Eco53kI GAGCTC 1 cut(s) 143
Eco57I CTGAAG 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 143
EcoNI CCTNNNNNAGG 2 cut(s) 25, 489
EcoRII CCWGG 2 cut(s) 129, 420
EcoT14I CCWWGG 1 cut(s) 490
EcoT22I ATGCAT 1 cut(s) 482
EcoT38I GRGCYC 2 cut(s) 145, 428
ErhI CCWWGG 1 cut(s) 490
FaeI CATG 1 cut(s) 573
FaiI YATR 5 cut(s) 393, 447, 482, 504, 571
FaqI GGGAC 1 cut(s) 359
FatI CATG 1 cut(s) 569
FauI CCCGC 1 cut(s) 284
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 285, 355
FokI GGATG 2 cut(s) 308, 338
FriOI GRGCYC 2 cut(s) 145, 428
Fsp4HI GCNGC 2 cut(s) 285, 355
FspBI CTAG 2 cut(s) 138, 438
GluI GCNGC 2 cut(s) 285, 355
GsuI CTGGAG 1 cut(s) 354
HapII CCGG 1 cut(s) 177
Hin1II CATG 1 cut(s) 573
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 1 cut(s) 495
HpaII CCGG 1 cut(s) 177
Hpy166II GTNNAC 3 cut(s) 238, 301, 313
Hpy188I TCNGA 2 cut(s) 153, 500
Hpy188III TCNNGA 3 cut(s) 10, 217, 429
Hpy8I GTNNAC 3 cut(s) 238, 301, 313
Hpy99I CGWCG 2 cut(s) 305, 353
HpyAV CCTTC 4 cut(s) 82, 94, 174, 454
HpyCH4V TGCA 3 cut(s) 378, 474, 480
HpyF10VI GCNNNNNNNGC 1 cut(s) 486
HpyF3I CTNAG 1 cut(s) 427
Hsp92II CATG 1 cut(s) 573
Kzo9I GATC 2 cut(s) 73, 186
LmnI GCTCC 1 cut(s) 148
MaeI CTAG 2 cut(s) 138, 438
MalI GATC 2 cut(s) 75, 188
MboI GATC 2 cut(s) 73, 186
MboII GAAGA 8 cut(s) 50, 53, 61, 97, 140, 150, 243, 557
MfeI CAATTG 2 cut(s) 243, 367
MhlI GDGCHC 2 cut(s) 145, 428
MluCI AATT 4 cut(s) 44, 172, 243, 367
MnlI CCTC 5 cut(s) 31, 156, 177, 329, 449
Mph1103I ATGCAT 1 cut(s) 482
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 1 cut(s) 276
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 2 cut(s) 131, 422
MunI CAATTG 2 cut(s) 243, 367
MvaI CCWGG 2 cut(s) 131, 422
MwoI GCNNNNNNNGC 1 cut(s) 486
NdeII GATC 2 cut(s) 73, 186
NlaIII CATG 1 cut(s) 573
NsiI ATGCAT 1 cut(s) 482
NspV TTCGAA 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 1 cut(s) 495
PfoI TCCNGGA 1 cut(s) 129
PkrI GCNGC 2 cut(s) 286, 356
Ple19I CGATCG 1 cut(s) 76
PsiI TTATAA 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 129, 420
PspGI CCWGG 2 cut(s) 129, 420
PvuI CGATCG 1 cut(s) 76
RsaI GTAC 2 cut(s) 331, 507
RsaNI GTAC 2 cut(s) 330, 506
SacI GAGCTC 1 cut(s) 145
SalI GTCGAC 1 cut(s) 299
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 2 cut(s) 285, 355
Sau3AI GATC 2 cut(s) 73, 186
ScrFI CCNGG 2 cut(s) 131, 422
SduI GDGCHC 2 cut(s) 145, 428
SetI ASST 9 cut(s) 63, 93, 99, 139, 145, 256, 282, 340, 487
SfuI TTCGAA 1 cut(s) 36
Sse9I AATT 4 cut(s) 44, 172, 243, 367
SsiI CCGC 3 cut(s) 284, 291, 354
SspI AATATT 1 cut(s) 265
SspMI CTAG 2 cut(s) 138, 438
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 2 cut(s) 129, 420
StyI CCWWGG 1 cut(s) 490
TaqI TCGA 4 cut(s) 36, 201, 229, 300
TasI AATT 4 cut(s) 44, 172, 243, 367
TatI WGTACW 1 cut(s) 505
TauI GCSGC 2 cut(s) 287, 357
TfiI GAWTC 1 cut(s) 495
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TspDTI ATGAA 1 cut(s) 408
TspGWI ACGGA 1 cut(s) 573
XagI CCTNNNNNAGG 2 cut(s) 25, 489
XapI RAATTY 1 cut(s) 172
XmiI GTMKAC 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 2 cut(s) 138, 438
Zsp2I ATGCAT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.