Rorug05G0575500

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014154
Physical Location & Seq
Forward (+)
77130110 .. 77131664
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0575500.1

Sequence Viewer

Length: 942 bp
ATGGGGTCTCAAACTCAGCCAGCTAAGGTTCCCATCGTAGATTTATCAAAAGAGGACTTGAAGCCTGGTACAGATGCATGGCTCTTGGCAAGCAAGGAAGTCATGTATGTCCTAGAAGAGCATGGTTGCTTTGAAGCTATTTACAATAAAGTTCCAATGGAACTTCACAACTCGACTATTTCTTTGCTTGAAGACCTTTTCAATCTTCCCTTGGAAACTAAGATGCAGAACACCAGTGACAGGCCTTACCACAGCTATATTGGACAGTATTCCTTTCTTCCTCTCTACGAATCCTTGGGTGTCGATAACCCGACGACATTAGAAGGAGCTCAACGCTTCACAAGTATCATGTGGCCTGAAGGAAATGACAAGTTTTGTGAAAGTGCTCATTCCTACTCAAAGCTGGTGACCGAATTGGGTGAAATGGTGACAAAAATGGTGTTTGAGAATTATGGTGTGGAGAGGCTCTATGAGTCTCACATGGCATCAACGACTTACCTCCTCAGATGCTCCAAATATAGAGCGCCTCAGCTGAATGAAACTGATATGGGATTGCTTCCTCACAAAGATAAGACCTTCTTATCTATACTTAATCAAAATGAGACAAATGGTTTGCAGATAAGAACAAAGGACGGCCAATGGATTGATGTGAAACCTTCACCTTCATCTTTTCTAGTCATGGCAGGAGACGCATTCATGGCATGGAGTAACGACAGGATACACTCTTGTGAACACCAAGTTGTCACGAAAGAGAAGAAAACCAGATACTCCTTGGGATTATTCGCATTTAATAGTGGGATCCTGCAAGTACCTGAAGAGTTAGTCGATGAAAAACACCCCTTGCTGTACAGGCCTTTCGATCATATTGGTTTCCTTTTTTTTAACAAGACACCAGAAGGAATGAAATCTGAGTGTCCTATCAAAGACTACTGTGGTGTTTGA

Protein Analysis

313

Amino Acids

35.74

Weight (kDa)

5.33

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 10 - 88 8e-08 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 170 - 262 1.1e-23 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19220
fragaria_vesca FvH4_2g06130 FvH4_2g06130
malus_domestica MD10G1094200.v1.1
prunus_persica Prupe.8G124000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0257061
rosa_laevigata RLG00000014674
rosa_multiflora Rmu_sc0000659.1_g000009 Rmu_sc0038017.1_g000001 Rmu_sc0038018.1_g000001
rosa_roxburghii Rroxscaffold_7G00208470
rosa_rugosa Rorug05G0575500
rosa_samantha Rh6AG093500 Rh6BG084300 Rh6CG081000 Rh6DG076400
rosa_wichuraiana Rw6G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 793, 806
AcoI YGGCCR 1 cut(s) 634
AcuI CTGAAG 2 cut(s) 378, 834
AfaI GTAC 3 cut(s) 70, 810, 848
AfiI CCNNNNNNNGG 1 cut(s) 240
AgsI TTSAA 4 cut(s) 61, 134, 191, 202
AjnI CCWGG 1 cut(s) 64
AleI CACNNNNGTG 1 cut(s) 726
AluBI AGCT 6 cut(s) 23, 137, 255, 329, 403, 532
AluI AGCT 6 cut(s) 23, 137, 255, 329, 403, 532
Alw21I GWGCWC 2 cut(s) 331, 388
Alw26I GTCTC 4 cut(s) 12, 480, 596, 681
AlwI GGATC 2 cut(s) 793, 806
AoxI GGCC 4 cut(s) 242, 353, 634, 851
ArsI GACNNNNNNTTYG 4 cut(s) 166, 198, 595, 627
AspLEI GCGC 1 cut(s) 526
AsuHPI GGTGA 4 cut(s) 418, 431, 439, 651
BamHI GGATCC 1 cut(s) 798
BanII GRGCYC 1 cut(s) 331
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 331, 388
BbvCI CCTCAGC 1 cut(s) 528
BccI CCATC 1 cut(s) 41
BceAI ACGGC 1 cut(s) 649
BciT130I CCWGG 1 cut(s) 66
BciVI GTATCC 1 cut(s) 711
BcoDI GTCTC 4 cut(s) 12, 480, 596, 681
BfaI CTAG 2 cut(s) 113, 674
BfoI RGCGCY 1 cut(s) 527
BfuI GTATCC 1 cut(s) 711
Bme1390I CCNGG 1 cut(s) 66
BmiI GGNNCC 2 cut(s) 30, 800
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 4 cut(s) 64, 213, 494, 497
BpiI GAAGAC 1 cut(s) 198
Bpu10I CCTNAGC 2 cut(s) 24, 528
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 210, 294, 771
Bsc4I CCNNNNNNNGG 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 234
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 3 cut(s) 210, 294, 771
BseLI CCNNNNNNNGG 1 cut(s) 240
BseMII CTCAG 4 cut(s) 29, 517, 542, 900
BseNI ACTGG 1 cut(s) 234
BseRI GAGGAG 1 cut(s) 491
BshFI GGCC 4 cut(s) 244, 355, 636, 853
BsiHKAI GWGCWC 2 cut(s) 331, 388
BslI CCNNNNNNNGG 1 cut(s) 240
BsmAI GTCTC 4 cut(s) 12, 480, 596, 681
BsmBI CGTCTC 1 cut(s) 681
BsmI GAATGC 1 cut(s) 692
BsnI GGCC 4 cut(s) 244, 355, 636, 853
Bso31I GGTCTC 1 cut(s) 12
Bsp1286I GDGCHC 2 cut(s) 331, 388
Bsp1407I TGTACA 1 cut(s) 846
Bsp143I GATC 2 cut(s) 798, 859
BspANI GGCC 4 cut(s) 244, 355, 636, 853
BspCNI CTCAG 4 cut(s) 28, 516, 541, 901
BspLI GGNNCC 2 cut(s) 30, 800
BspPI GGATC 2 cut(s) 793, 806
BspQI GCTCTTC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 846
BsrI ACTGG 1 cut(s) 234
BssECI CCNNGG 3 cut(s) 210, 294, 771
BssMI GATC 2 cut(s) 798, 859
BssT1I CCWWGG 3 cut(s) 210, 294, 771
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 2 cut(s) 267, 932
Bst6I CTCTTC 2 cut(s) 111, 810
BstAUI TGTACA 1 cut(s) 846
BstC8I GCNNGC 2 cut(s) 21, 91
BstDEI CTNAG 6 cut(s) 15, 24, 219, 503, 528, 909
BstEII GGTNACC 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 527
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 2 cut(s) 801, 862
BstMAI GTCTC 4 cut(s) 12, 480, 596, 681
BstMBI GATC 2 cut(s) 798, 859
BstMWI GCNNNNNNNGC 3 cut(s) 689, 698, 850
BstNI CCWGG 1 cut(s) 66
BstPI GGTNACC 1 cut(s) 406
BstSCI CCNGG 1 cut(s) 64
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 798
BstYI RGATCY 1 cut(s) 798
BsuI GTATCC 1 cut(s) 711
BsuRI GGCC 4 cut(s) 244, 355, 636, 853
BtsIMutI CAGTG 1 cut(s) 241
Cac8I GCNNGC 2 cut(s) 21, 91
CfoI GCGC 1 cut(s) 526
CseI GACGC 1 cut(s) 698
Csp6I GTAC 3 cut(s) 69, 809, 847
CviAII CATG 8 cut(s) 78, 103, 122, 349, 481, 679, 697, 702
CviQI GTAC 3 cut(s) 69, 809, 847
DdeI CTNAG 6 cut(s) 15, 24, 219, 503, 528, 909
DpnI GATC 2 cut(s) 800, 861
DpnII GATC 2 cut(s) 798, 859
EaeI YGGCCR 1 cut(s) 634
Eam1104I CTCTTC 2 cut(s) 111, 810
EarI CTCTTC 2 cut(s) 111, 810
Ecl136II GAGCTC 1 cut(s) 329
Eco130I CCWWGG 3 cut(s) 210, 294, 771
Eco147I AGGCCT 2 cut(s) 244, 853
Eco24I GRGCYC 1 cut(s) 331
Eco31I GGTCTC 1 cut(s) 12
Eco53kI GAGCTC 1 cut(s) 329
Eco57I CTGAAG 2 cut(s) 378, 834
Eco91I GGTNACC 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 329
EcoO65I GGTNACC 1 cut(s) 406
EcoRII CCWGG 1 cut(s) 64
EcoT14I CCWWGG 3 cut(s) 210, 294, 771
EcoT22I ATGCAT 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 331
ErhI CCWWGG 3 cut(s) 210, 294, 771
Esp3I CGTCTC 1 cut(s) 681
FaeI CATG 8 cut(s) 81, 106, 125, 352, 484, 682, 700, 705
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FatI CATG 8 cut(s) 77, 102, 121, 348, 480, 678, 696, 701
FriOI GRGCYC 1 cut(s) 331
FspBI CTAG 2 cut(s) 113, 674
GlaI GCGC 1 cut(s) 525
HaeII RGCGCY 1 cut(s) 527
HaeIII GGCC 4 cut(s) 244, 355, 636, 853
HgaI GACGC 1 cut(s) 698
HhaI GCGC 1 cut(s) 526
Hin1II CATG 8 cut(s) 81, 106, 125, 352, 484, 682, 700, 705
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HinfI GANTC 2 cut(s) 290, 473
HphI GGTGA 4 cut(s) 418, 431, 439, 651
Hpy166II GTNNAC 1 cut(s) 731
Hpy188I TCNGA 2 cut(s) 506, 910
Hpy188III TCNNGA 1 cut(s) 745
Hpy8I GTNNAC 1 cut(s) 731
Hpy99I CGWCG 1 cut(s) 316
HpyAV CCTTC 6 cut(s) 317, 353, 586, 666, 672, 890
HpyCH4III ACNGT 2 cut(s) 267, 932
HpyCH4V TGCA 4 cut(s) 77, 226, 616, 805
HpyF10VI GCNNNNNNNGC 3 cut(s) 689, 698, 850
HpyF3I CTNAG 6 cut(s) 15, 24, 219, 503, 528, 909
Hsp92II CATG 8 cut(s) 81, 106, 125, 352, 484, 682, 700, 705
HspAI GCGC 1 cut(s) 524
Kzo9I GATC 2 cut(s) 798, 859
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 2 cut(s) 326, 515
LweI GCATC 4 cut(s) 64, 213, 494, 497
MaeI CTAG 2 cut(s) 113, 674
MaeIII GTNAC 5 cut(s) 236, 406, 427, 707, 742
MalI GATC 2 cut(s) 800, 861
MboI GATC 2 cut(s) 798, 859
MboII GAAGA 6 cut(s) 128, 197, 203, 269, 766, 827
MflI RGATCY 1 cut(s) 798
MhlI GDGCHC 2 cut(s) 331, 388
MluCI AATT 2 cut(s) 413, 448
MlyI GAGTC 1 cut(s) 482
MnlI CCTC 7 cut(s) 46, 291, 456, 509, 512, 537, 570
Mph1103I ATGCAT 1 cut(s) 79
MseI TTAA 3 cut(s) 591, 789, 882
MslI CAYNNNNRTG 1 cut(s) 726
MspA1I CMGCKG 1 cut(s) 532
MspR9I CCNGG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 692
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 3 cut(s) 689, 698, 850
NdeII GATC 2 cut(s) 798, 859
NlaIII CATG 8 cut(s) 81, 106, 125, 352, 484, 682, 700, 705
NlaIV GGNNCC 2 cut(s) 30, 800
NmuCI GTSAC 4 cut(s) 236, 406, 427, 742
NsiI ATGCAT 1 cut(s) 79
OliI CACNNNNGTG 1 cut(s) 726
PceI AGGCCT 2 cut(s) 244, 853
PciSI GCTCTTC 1 cut(s) 111
PctI GAATGC 1 cut(s) 692
PfeI GAWTC 1 cut(s) 290
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
Psp124BI GAGCTC 1 cut(s) 331
Psp6I CCWGG 1 cut(s) 64
PspEI GGTNACC 1 cut(s) 406
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 2 cut(s) 30, 800
PsuI RGATCY 1 cut(s) 798
PvuII CAGCTG 1 cut(s) 532
RsaI GTAC 3 cut(s) 70, 810, 848
RsaNI GTAC 3 cut(s) 69, 809, 847
RseI CAYNNNNRTG 1 cut(s) 726
SacI GAGCTC 1 cut(s) 331
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 3 cut(s) 591, 789, 882
Sau3AI GATC 2 cut(s) 798, 859
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 2 cut(s) 331, 388
SfaNI GCATC 4 cut(s) 64, 213, 494, 497
SmiMI CAYNNNNRTG 1 cut(s) 726
Sse9I AATT 2 cut(s) 413, 448
SseBI AGGCCT 2 cut(s) 244, 853
SspMI CTAG 2 cut(s) 113, 674
SstI GAGCTC 1 cut(s) 331
StuI AGGCCT 2 cut(s) 244, 853
StyD4I CCNGG 1 cut(s) 64
StyI CCWWGG 3 cut(s) 210, 294, 771
TaaI ACNGT 2 cut(s) 267, 932
TaqI TCGA 4 cut(s) 173, 303, 825, 858
TaqII GACCGA 1 cut(s) 425
TasI AATT 2 cut(s) 413, 448
TatI WGTACW 1 cut(s) 846
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 3 cut(s) 591, 789, 882
Tru9I TTAA 3 cut(s) 591, 789, 882
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 4 cut(s) 236, 406, 427, 742
Tsp45I GTSAC 4 cut(s) 236, 406, 427, 742
TspDTI ATGAA 5 cut(s) 552, 654, 685, 843, 917
TspRI CASTG 1 cut(s) 241
XcmI CCANNNNNNNNNTGG 2 cut(s) 257, 769
XspI CTAG 2 cut(s) 113, 674
Zsp2I ATGCAT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.