RLG00000014674

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014154
Physical Location & Seq
Reverse (-)
56063676 .. 56066268
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014674

Sequence Viewer

Length: 585 bp
ATGTTGGCTACTAACCCTAGCCTCTTCTTCCCCTCCAAACCCTCCATTGCCCTCCGCTGCCGCGCCGCCGCCGGCGACCCTCCTCGAGGCGCCGCATTCCCTCGCCTGGTCCAGTTTCCCTTCGCCGCCTCCGACGTCGCCTCCGTCAGCGGCCGAATCGCCCTTGACACCGAACCCGACGGCGGCGGAGCGGCTCGGCTTCGCGGCGGAAGCTCCGGCGGTGCTATTAAGGTGACGGCCAAGGAGCCGAAGTGGTCGCGGGATAGAGAGAGTTACTTGGTCGACGATGGAGACGCGCTGCCTCTCCCCATGACTTATCCAGATACGGCTCCGGTGACGCCGGAGGTGATTGACCAGAGGCTCCGGTGCGACCCCGTAGTTGAGGATTGCAAGATAGTAGTGTATGAATGGACAGGAAAGTGTAGGAGTTGCCAAGGCTCAGGATATGCTAGTTATTCTAATAAAAGAGGGAAGAAAAATGTTTGCAAATGCATACCTTGCCAAGGAATCGGATATGTACAAAAGATAACAACTCGCACCAACATTGATGTAATGGAAGATTTGGATAACGGAAAACCACCATGA

Protein Analysis

195

Amino Acids

20.83

Weight (kDa)

8.61

Isoelectric Point (pI)

29.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19220
fragaria_vesca FvH4_2g06130 FvH4_2g06130
malus_domestica MD10G1094200.v1.1
prunus_persica Prupe.8G124000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0257061
rosa_laevigata RLG00000014674
rosa_multiflora Rmu_sc0000659.1_g000009 Rmu_sc0038017.1_g000001 Rmu_sc0038018.1_g000001
rosa_roxburghii Rroxscaffold_7G00208470
rosa_rugosa Rorug05G0575500
rosa_samantha Rh6AG093500 Rh6BG084300 Rh6CG081000 Rh6DG076400
rosa_wichuraiana Rw6G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AbsI CCTCGAGG 1 cut(s) 84
AccB1I GGYRCC 1 cut(s) 89
AccBSI CCGCTC 1 cut(s) 191
AccI GTMKAC 1 cut(s) 282
AccII CGCG 4 cut(s) 63, 204, 259, 296
AcoI YGGCCR 2 cut(s) 151, 237
AcyI GRCGYC 3 cut(s) 90, 135, 338
AfaI GTAC 1 cut(s) 519
AfiI CCNNNNNNNGG 4 cut(s) 86, 106, 182, 503
AjnI CCWGG 1 cut(s) 105
AluBI AGCT 1 cut(s) 213
AluI AGCT 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 285
Ama87I CYCGRG 1 cut(s) 84
AoxI GGCC 2 cut(s) 151, 237
ApeKI GCWGC 2 cut(s) 57, 298
AspLEI GCGC 3 cut(s) 65, 92, 298
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 3 cut(s) 244, 346, 358
AvaI CYCGRG 1 cut(s) 84
AvaII GGWCC 1 cut(s) 109
BanI GGYRCC 1 cut(s) 89
BbvI GCAGC 2 cut(s) 44, 285
BccI CCATC 1 cut(s) 281
BceAI ACGGC 3 cut(s) 196, 252, 342
BciT130I CCWGG 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 2 cut(s) 18, 450
BfoI RGCGCY 1 cut(s) 93
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 4 cut(s) 91, 246, 330, 362
BmrFI CCNGG 1 cut(s) 107
Bpu10I CCTNAGC 1 cut(s) 439
BsaHI GRCGYC 3 cut(s) 90, 135, 338
BsaJI CCNNGG 3 cut(s) 240, 433, 502
BsaWI WCCGGW 2 cut(s) 331, 363
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 236, 266
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 106, 182, 503
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 107
BseDI CCNNGG 3 cut(s) 240, 433, 502
BseLI CCNNNNNNNGG 4 cut(s) 86, 106, 182, 503
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 1 cut(s) 112
BseRI GAGGAG 1 cut(s) 72
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 44, 285
Bsh1236I CGCG 4 cut(s) 63, 204, 259, 296
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 2 cut(s) 153, 239
BshNI GGYRCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 5 cut(s) 72, 216, 332, 341, 364
BslI CCNNNNNNNGG 4 cut(s) 86, 106, 182, 503
BsmAI GTCTC 1 cut(s) 285
BsmBI CGTCTC 1 cut(s) 285
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 2 cut(s) 153, 239
BsoBI CYCGRG 1 cut(s) 84
Bsp1407I TGTACA 1 cut(s) 517
BspANI GGCC 2 cut(s) 153, 239
BspCNI CTCAG 1 cut(s) 452
BspFNI CGCG 4 cut(s) 63, 204, 259, 296
BspLI GGNNCC 4 cut(s) 91, 246, 330, 362
BspT107I GGYRCC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 517
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 3 cut(s) 240, 433, 502
BssNI GRCGYC 3 cut(s) 90, 135, 338
BssT1I CCWWGG 3 cut(s) 240, 433, 502
Bst2UI CCWGG 1 cut(s) 107
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 3 cut(s) 90, 135, 338
BstAPI GCANNNNNTGC 1 cut(s) 498
BstAUI TGTACA 1 cut(s) 517
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 1 cut(s) 439
BstENI CCTNNNNNAGG 2 cut(s) 84, 501
BstFNI CGCG 4 cut(s) 63, 204, 259, 296
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 3 cut(s) 65, 92, 298
BstMAI GTCTC 1 cut(s) 285
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 2 cut(s) 210, 498
BstNI CCWGG 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 105
BstUI CGCG 4 cut(s) 63, 204, 259, 296
BstV1I GCAGC 2 cut(s) 44, 285
BstZI CGGCCG 1 cut(s) 151
BsuRI GGCC 2 cut(s) 153, 239
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 3 cut(s) 65, 92, 298
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 2 cut(s) 302, 346
Csp6I GTAC 1 cut(s) 518
CviAII CATG 2 cut(s) 310, 582
CviQI GTAC 1 cut(s) 518
DdeI CTNAG 1 cut(s) 439
DinI GGCGCC 1 cut(s) 91
EaeI YGGCCR 2 cut(s) 151, 237
EagI CGGCCG 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 2 cut(s) 201, 222
EclXI CGGCCG 1 cut(s) 151
Eco130I CCWWGG 3 cut(s) 240, 433, 502
Eco47I GGWCC 1 cut(s) 109
Eco52I CGGCCG 1 cut(s) 151
Eco88I CYCGRG 1 cut(s) 84
EcoNI CCTNNNNNAGG 2 cut(s) 84, 501
EcoRII CCWGG 1 cut(s) 105
EcoT14I CCWWGG 3 cut(s) 240, 433, 502
EcoT22I ATGCAT 1 cut(s) 494
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
ErhI CCWWGG 3 cut(s) 240, 433, 502
Esp3I CGTCTC 1 cut(s) 285
FaeI CATG 2 cut(s) 313, 585
FaiI YATR 6 cut(s) 311, 405, 447, 494, 516, 583
FatI CATG 2 cut(s) 309, 581
FauI CCCGC 1 cut(s) 252
FblI GTMKAC 1 cut(s) 282
FspBI CTAG 2 cut(s) 18, 450
GlaI GCGC 3 cut(s) 64, 91, 297
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 2 cut(s) 153, 239
HapII CCGG 5 cut(s) 72, 216, 332, 341, 364
HgaI GACGC 2 cut(s) 302, 346
HhaI GCGC 3 cut(s) 65, 92, 298
Hin1I GRCGYC 3 cut(s) 90, 135, 338
Hin1II CATG 2 cut(s) 313, 585
Hin6I GCGC 3 cut(s) 63, 90, 296
HinP1I GCGC 3 cut(s) 63, 90, 296
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 2 cut(s) 156, 507
HpaII CCGG 5 cut(s) 72, 216, 332, 341, 364
HphI GGTGA 3 cut(s) 244, 346, 358
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 2 cut(s) 133, 512
Hpy188III TCNNGA 2 cut(s) 320, 441
Hpy8I GTNNAC 1 cut(s) 283
Hpy99I CGWCG 4 cut(s) 137, 140, 182, 287
HpyAV CCTTC 1 cut(s) 130
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 3 cut(s) 390, 486, 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 498
HpyF3I CTNAG 1 cut(s) 439
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 3 cut(s) 90, 135, 338
Hsp92II CATG 2 cut(s) 313, 585
HspAI GCGC 3 cut(s) 63, 90, 296
KasI GGCGCC 1 cut(s) 89
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
LmnI GCTCC 5 cut(s) 188, 218, 244, 334, 366
Lsp1109I GCAGC 2 cut(s) 44, 285
MaeI CTAG 2 cut(s) 18, 450
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 3 cut(s) 232, 272, 334
MbiI CCGCTC 1 cut(s) 191
MboII GAAGA 4 cut(s) 16, 19, 484, 569
Mly113I GGCGCC 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 156
Mph1103I ATGCAT 1 cut(s) 494
MreI CGCCGGCG 1 cut(s) 71
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 1 cut(s) 228
MspA1I CMGCKG 2 cut(s) 57, 150
MspI CCGG 5 cut(s) 72, 216, 332, 341, 364
MspR9I CCNGG 1 cut(s) 107
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 1 cut(s) 107
MvnI CGCG 4 cut(s) 63, 204, 259, 296
MwoI GCNNNNNNNGC 2 cut(s) 210, 498
NaeI GCCGGC 1 cut(s) 73
NarI GGCGCC 1 cut(s) 90
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 2 cut(s) 313, 585
NlaIV GGNNCC 4 cut(s) 91, 246, 330, 362
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 2 cut(s) 232, 334
NsiI ATGCAT 1 cut(s) 494
PaeR7I CTCGAG 1 cut(s) 84
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 141
PctI GAATGC 1 cut(s) 95
PdiI GCCGGC 1 cut(s) 73
PfeI GAWTC 2 cut(s) 156, 507
PluTI GGCGCC 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 4 cut(s) 91, 246, 330, 362
PspPI GGNCC 1 cut(s) 109
PspXI VCTCGAGB 1 cut(s) 84
RsaI GTAC 1 cut(s) 519
RsaNI GTAC 1 cut(s) 518
SalI GTCGAC 1 cut(s) 281
SaqAI TTAA 1 cut(s) 228
Sau96I GGNCC 1 cut(s) 109
ScrFI CCNGG 1 cut(s) 107
SetI ASST 5 cut(s) 138, 215, 234, 348, 499
SfoI GGCGCC 1 cut(s) 91
Sfr274I CTCGAG 1 cut(s) 84
SgrAI CRCCGGYG 1 cut(s) 71
SinI GGWCC 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 2 cut(s) 18, 450
StyD4I CCNGG 1 cut(s) 105
StyI CCWWGG 3 cut(s) 240, 433, 502
TaiI ACGT 1 cut(s) 138
TaqI TCGA 2 cut(s) 85, 282
TatI WGTACW 1 cut(s) 517
TauI GCSGC 9 cut(s) 63, 68, 71, 95, 128, 153, 186, 194, 207
TfiI GAWTC 2 cut(s) 156, 507
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseFI GTSAC 2 cut(s) 232, 334
TseI GCWGC 2 cut(s) 57, 298
Tsp45I GTSAC 2 cut(s) 232, 334
TspDTI ATGAA 1 cut(s) 420
TspGWI ACGGA 2 cut(s) 133, 585
VpaK11BI GGWCC 1 cut(s) 109
XagI CCTNNNNNAGG 2 cut(s) 84, 501
XhoI CTCGAG 1 cut(s) 84
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 2 cut(s) 18, 450
ZraI GACGTC 1 cut(s) 136
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.