FvH4_2g06711

nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015047
Physical Location & Seq
Reverse (-)
5562456 .. 5565491
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06711.t1

Sequence Viewer

Length: 1404 bp
ATGAGTTCTTCTAGTAGTTCACAAGCTTATGAAGCCACGGATGCCAAGAACAAGACATCAACAACTCCAAAAGACAAAGAATCCAGACTGCCTAAAGCAGAGAAAGTTGAGTGTCCGCCAGCTCCGAAGCGTCCACCTCCAAAGAGTGGGCATAATGATTCTTTTAGCCCACTTGATTTGAACACAAGATGTGGAGACAGTCAAGAGAGCCGCACCCTGAGCCATGGATCAATCCTTCCTCCTGATGCTCTCAAACCTCCTCCACCTCCACAACTCCATCGACCCAACCACCTCCCTCATCTCCTCCCCCACCTCCACCTCCTCCTCCCCCACCTCCCTCCTCACCTCCTCCTCCGCCGCCCCTCTCCTCTTCTTCACCATCGCCTCCGTCCTCTCCTACATCGCCTCCTCCCGCCCTCCTCCTCCTCCTCCTCCTCCTCTTCCTCCCCCGCCGCCTCCGACTACTCCGTCTCCGCCTTCCGCGCCCTCTCCACCGAGCACATCTGGTCCCTCGAAGCCCCTCTCCGCGACGCCCAGTGGCGCTCCCTCTACGGCTTGTCGTATCCCGTCTTTACGACCGTCGTCGATAAGCTCAAACCCCACATTGCTCTCTCCAACCTCTCCCTCCCTTCCGACTACGCCGTCGCCATGGTCCTCTCCCGCCTCACCCACGGCTTCTCCGCCAAGTCCCTCGCCGCTCGCTACTCCCTCGAGCCCTACCTGATCTCCAAGATCACCAACATGGTCACGCGCCTACTCGCCACCAAGCTCTACCCGGAGTTCATCAAGATCCCCGTCAGCCGCCGCAGGCTCATCGAAACCACCCAGGCCTTCGAGGAGCTCACGTCACTCCCCAATATGTGCGGCGCCATTGACGGGAGTCCGATTCGTCTGCACAAGCAGAATCTGTCTGGGAATTACAAATGCCGGTACGGCTACAATGCTGTCCAGCTGGAGGTTGTTGCTGACCACAAGAAGATCTTCTGGGATGTCTGTGTTAAAGCTCCGGGAGGCACCGACGACGCCGCGCATTTCCGAGAGAGTTTGCTGTATAACAGGCTTACTTCCGGTGACATTGTGTGGGATAAGAACTTGTTCGATGGAATGCTCATGAAGGGGAGGTCTGTGGTGGTTGAGGCGATTGGGTTGCTCAAAGGGAGGTGGAAGATTCTGCAGGACCTGAATGTCGGTCTTAATCATGTCCCGCAGACAATTGTTGCTTGCTGTGTGCTGCATAATCTGTGCCAGATTGCGAGGGAGCCTGAACCGGAGCCGTGGAAGGACCCTGAGGAGAATGGGATGCCTCCGAGGGTGCTTGACAGTGAGAAGTCGTTTTACTACTTTGGGGAAAGCTTGAGGCAAGCATTGGCTGATGATTTGCACCAGAGGCTTTCATCGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

468

Amino Acids

52.17

Weight (kDa)

9.17

Isoelectric Point (pI)

61.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 371 - 413 6.7e-06 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19120
fragaria_vesca FvH4_2g06711
prunus_persica Prupe.8G119100_v2.0.a1
pyrus_communis pycom05g06910
rosa_chinensis RchiOBHm_Chr6g0258821
rosa_laevigata RLG00000014583
rosa_multiflora Rmu_co8359799.1_g000001 Rmu_sc0004485.1_g000014
rosa_roxburghii Rroxscaffold_7G00206090
rosa_rugosa Rorug05G0583500 Rorug05G0583600
rosa_samantha Rh6AG100100 Rh6BG091600 Rh6CG089100 Rh6DG083400
rosa_wichuraiana Rw6G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 467, 641, 1184
AccB1I GGYRCC 2 cut(s) 866, 1013
AccB7I CCANNNNNTGG 1 cut(s) 146
AccBSI CCGCTC 1 cut(s) 698
AccII CGCG 4 cut(s) 483, 528, 751, 1028
AclWI GGATC 2 cut(s) 235, 784
AcyI GRCGYC 3 cut(s) 531, 867, 1023
AfaI GTAC 1 cut(s) 932
AfiI CCNNNNNNNGG 3 cut(s) 146, 876, 955
AgsI TTSAA 1 cut(s) 181
AjiI CACGTC 1 cut(s) 846
AjnI CCWGG 1 cut(s) 825
AluBI AGCT 8 cut(s) 26, 122, 592, 769, 841, 952, 1004, 1353
AluI AGCT 8 cut(s) 26, 122, 592, 769, 841, 952, 1004, 1353
Alw21I GWGCWC 2 cut(s) 501, 843
Alw26I GTCTC 2 cut(s) 189, 475
AlwI GGATC 2 cut(s) 235, 784
AlwNI CAGNNNCTG 2 cut(s) 907, 1180
Ama87I CYCGRG 1 cut(s) 710
AoxI GGCC 1 cut(s) 828
ApeKI GCWGC 1 cut(s) 1231
Asp700I GAANNNNTTC 2 cut(s) 980, 1094
AspLEI GCGC 5 cut(s) 485, 543, 753, 869, 1030
AspS9I GGNCC 4 cut(s) 507, 652, 1177, 1282
AsuC2I CCSGG 2 cut(s) 776, 1008
AsuHPI GGTGA 5 cut(s) 335, 368, 658, 727, 1082
AvaI CYCGRG 1 cut(s) 710
AvaII GGWCC 4 cut(s) 507, 652, 1177, 1282
AxyI CCTNAGG 1 cut(s) 1287
BanI GGYRCC 2 cut(s) 866, 1013
BanII GRGCYC 2 cut(s) 717, 843
BarI GAAGNNNNNNTAC 2 cut(s) 1319, 1351
Bbv12I GWGCWC 2 cut(s) 501, 843
BbvI GCAGC 1 cut(s) 1218
BccI CCATC 3 cut(s) 285, 387, 1094
BceAI ACGGC 5 cut(s) 568, 626, 688, 949, 1258
BcgI CGANNNNNNTGC 6 cut(s) 796, 830, 874, 908, 1129, 1163
BciT130I CCWGG 1 cut(s) 827
BciVI GTATCC 1 cut(s) 573
BcnI CCSGG 2 cut(s) 776, 1008
BcoDI GTCTC 2 cut(s) 189, 475
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 1172
BfoI RGCGCY 2 cut(s) 544, 870
BfuI GTATCC 1 cut(s) 573
BglI GCCNNNNNGGC 1 cut(s) 933
BglII AGATCT 1 cut(s) 978
BisI GCNGC 9 cut(s) 211, 358, 453, 696, 802, 805, 865, 1026, 1232
BlsI GCNGC 9 cut(s) 212, 359, 454, 697, 803, 806, 866, 1027, 1233
Bme1390I CCNGG 3 cut(s) 776, 827, 1008
Bme18I GGWCC 4 cut(s) 507, 652, 1177, 1282
BmeT110I CYCGRG 1 cut(s) 710
BmgBI CACGTC 1 cut(s) 846
BmgT120I GGNCC 4 cut(s) 507, 652, 1177, 1282
BmiI GGNNCC 6 cut(s) 509, 868, 1015, 1260, 1272, 1284
BmrFI CCNGG 3 cut(s) 776, 827, 1008
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 3 cut(s) 31, 235, 1290
BmuI ACTGGG 1 cut(s) 529
BoxI GACNNNNGTC 2 cut(s) 581, 879
BpmI CTGGAG 1 cut(s) 974
Bpu10I CCTNAGC 1 cut(s) 218
BpuEI CTTGAG 1 cut(s) 1375
BpuMI CCSGG 2 cut(s) 776, 1008
BsaHI GRCGYC 3 cut(s) 531, 867, 1023
BsaJI CCNNGG 7 cut(s) 36, 223, 648, 670, 825, 1274, 1307
BsaWI WCCGGW 2 cut(s) 1067, 1267
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 876, 955
Bse118I RCCGGY 1 cut(s) 927
Bse1I ACTGG 1 cut(s) 535
Bse21I CCTNAGG 1 cut(s) 1287
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 1 cut(s) 827
BseDI CCNNGG 7 cut(s) 36, 223, 648, 670, 825, 1274, 1307
BseGI GGATG 3 cut(s) 46, 994, 1305
BseLI CCNNNNNNNGG 3 cut(s) 146, 876, 955
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 2 cut(s) 209, 1278
BseNI ACTGG 1 cut(s) 535
BseXI GCAGC 1 cut(s) 1218
BsgI GTGCAG 1 cut(s) 878
Bsh1236I CGCG 4 cut(s) 483, 528, 751, 1028
Bsh1285I CGRYCG 1 cut(s) 579
BshFI GGCC 1 cut(s) 830
BshNI GGYRCC 2 cut(s) 866, 1013
BsiEI CGRYCG 1 cut(s) 579
BsiHKAI GWGCWC 2 cut(s) 501, 843
BsiHKCI CYCGRG 1 cut(s) 710
BsiSI CCGG 5 cut(s) 776, 928, 1007, 1068, 1268
BslFI GGGAC 3 cut(s) 493, 673, 1187
BslI CCNNNNNNNGG 3 cut(s) 146, 876, 955
BsmAI GTCTC 2 cut(s) 189, 475
BsmBI CGTCTC 1 cut(s) 475
BsmFI GGGAC 3 cut(s) 493, 673, 1187
BsmI GAATGC 1 cut(s) 1110
BsnI GGCC 1 cut(s) 830
BsoBI CYCGRG 1 cut(s) 710
Bsp1286I GDGCHC 3 cut(s) 501, 717, 843
Bsp143I GATC 5 cut(s) 227, 723, 732, 789, 978
Bsp19I CCATGG 2 cut(s) 223, 648
BspANI GGCC 1 cut(s) 830
BspCNI CTCAG 2 cut(s) 210, 1279
BspFNI CGCG 4 cut(s) 483, 528, 751, 1028
BspHI TCATGA 1 cut(s) 1110
BspLI GGNNCC 6 cut(s) 509, 868, 1015, 1260, 1272, 1284
BspMAI CTGCAG 1 cut(s) 1176
BspPI GGATC 2 cut(s) 235, 784
BspT107I GGYRCC 2 cut(s) 866, 1013
BsrBI CCGCTC 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 603
BsrFI RCCGGY 1 cut(s) 927
BsrI ACTGG 1 cut(s) 535
BssAI RCCGGY 1 cut(s) 927
BssECI CCNNGG 7 cut(s) 36, 223, 648, 670, 825, 1274, 1307
BssMI GATC 5 cut(s) 227, 723, 732, 789, 978
BssNI GRCGYC 3 cut(s) 531, 867, 1023
BssT1I CCWWGG 2 cut(s) 223, 648
Bst2UI CCWGG 1 cut(s) 827
Bst4CI ACNGT 3 cut(s) 200, 580, 1322
Bst6I CTCTTC 2 cut(s) 375, 445
BstACI GRCGYC 3 cut(s) 531, 867, 1023
BstC8I GCNNGC 5 cut(s) 120, 700, 809, 1222, 1362
BstDEI CTNAG 2 cut(s) 218, 1287
BstDSI CCRYGG 5 cut(s) 36, 223, 648, 670, 1274
BstF5I GGATG 3 cut(s) 46, 994, 1305
BstFNI CGCG 4 cut(s) 483, 528, 751, 1028
BstH2I RGCGCY 2 cut(s) 544, 870
BstHHI GCGC 5 cut(s) 485, 543, 753, 869, 1030
BstKTI GATC 5 cut(s) 230, 726, 735, 792, 981
BstMAI GTCTC 2 cut(s) 189, 475
BstMBI GATC 5 cut(s) 227, 723, 732, 789, 978
BstMCI CGRYCG 1 cut(s) 579
BstMWI GCNNNNNNNGC 6 cut(s) 32, 41, 219, 482, 933, 1387
BstNI CCWGG 1 cut(s) 827
BstPAI GACNNNNGTC 2 cut(s) 581, 879
BstSCI CCNGG 3 cut(s) 774, 825, 1006
BstSFI CTRYAG 1 cut(s) 1172
BstUI CGCG 4 cut(s) 483, 528, 751, 1028
BstV1I GCAGC 1 cut(s) 1218
BstX2I RGATCY 2 cut(s) 789, 978
BstYI RGATCY 2 cut(s) 789, 978
Bsu36I CCTNAGG 1 cut(s) 1287
BsuI GTATCC 1 cut(s) 573
BsuRI GGCC 1 cut(s) 830
BtgI CCRYGG 5 cut(s) 36, 223, 648, 670, 1274
BtgZI GCGATG 2 cut(s) 365, 386
BtrI CACGTC 1 cut(s) 846
BtsCI GGATG 3 cut(s) 46, 994, 1305
BtsIMutI CAGTG 2 cut(s) 542, 1327
Cac8I GCNNGC 5 cut(s) 120, 700, 809, 1222, 1362
CaiI CAGNNNCTG 2 cut(s) 907, 1180
CciI TCATGA 1 cut(s) 1110
CfoI GCGC 5 cut(s) 485, 543, 753, 869, 1030
Cfr10I RCCGGY 1 cut(s) 927
Cfr13I GGNCC 4 cut(s) 507, 652, 1177, 1282
CseI GACGC 3 cut(s) 119, 539, 1031
Csp6I GTAC 1 cut(s) 931
CspCI CAANNNNNGTGG 2 cut(s) 159, 194
CviAII CATG 5 cut(s) 224, 649, 742, 1111, 1199
CviQI GTAC 1 cut(s) 931
DdeI CTNAG 2 cut(s) 218, 1287
DinI GGCGCC 1 cut(s) 868
DpnI GATC 5 cut(s) 229, 725, 734, 791, 980
DpnII GATC 5 cut(s) 227, 723, 732, 789, 978
DrdI GACNNNNNNGTC 3 cut(s) 467, 641, 1184
DseDI GACNNNNNNGTC 3 cut(s) 467, 641, 1184
Eam1104I CTCTTC 2 cut(s) 375, 445
EarI CTCTTC 2 cut(s) 375, 445
EciI GGCGGA 4 cut(s) 105, 344, 463, 670
Ecl136II GAGCTC 1 cut(s) 841
Eco130I CCWWGG 2 cut(s) 223, 648
Eco147I AGGCCT 1 cut(s) 830
Eco24I GRGCYC 2 cut(s) 717, 843
Eco47I GGWCC 4 cut(s) 507, 652, 1177, 1282
Eco53kI GAGCTC 1 cut(s) 841
Eco81I CCTNAGG 1 cut(s) 1287
Eco88I CYCGRG 1 cut(s) 710
EcoICRI GAGCTC 1 cut(s) 841
EcoO109I RGGNCCY 2 cut(s) 1177, 1282
EcoRII CCWGG 1 cut(s) 825
EcoT14I CCWWGG 2 cut(s) 223, 648
EcoT38I GRGCYC 2 cut(s) 717, 843
EgeI GGCGCC 1 cut(s) 868
EheI GGCGCC 1 cut(s) 868
ErhI CCWWGG 2 cut(s) 223, 648
Esp3I CGTCTC 1 cut(s) 475
FaeI CATG 5 cut(s) 227, 652, 745, 1114, 1202
FalI AAGNNNNNCTT 2 cut(s) 965, 997
FaqI GGGAC 3 cut(s) 493, 673, 1187
FatI CATG 5 cut(s) 223, 648, 741, 1110, 1198
FauI CCCGC 4 cut(s) 420, 457, 668, 1212
Fnu4HI GCNGC 9 cut(s) 211, 358, 453, 696, 802, 805, 865, 1026, 1232
FokI GGATG 3 cut(s) 53, 1001, 1312
FriOI GRGCYC 2 cut(s) 717, 843
Fsp4HI GCNGC 9 cut(s) 211, 358, 453, 696, 802, 805, 865, 1026, 1232
FspBI CTAG 1 cut(s) 12
GlaI GCGC 5 cut(s) 484, 542, 752, 868, 1029
GluI GCNGC 9 cut(s) 211, 358, 453, 696, 802, 805, 865, 1026, 1232
GsuI CTGGAG 1 cut(s) 974
HaeII RGCGCY 2 cut(s) 544, 870
HaeIII GGCC 1 cut(s) 830
HapII CCGG 5 cut(s) 776, 928, 1007, 1068, 1268
HgaI GACGC 3 cut(s) 119, 539, 1031
HhaI GCGC 5 cut(s) 485, 543, 753, 869, 1030
Hin1I GRCGYC 3 cut(s) 531, 867, 1023
Hin1II CATG 5 cut(s) 227, 652, 745, 1114, 1202
Hin6I GCGC 5 cut(s) 483, 541, 751, 867, 1028
HinP1I GCGC 5 cut(s) 483, 541, 751, 867, 1028
HindIII AAGCTT 2 cut(s) 24, 1351
HinfI GANTC 6 cut(s) 80, 158, 880, 886, 904, 1168
HpaII CCGG 5 cut(s) 776, 928, 1007, 1068, 1268
HphI GGTGA 5 cut(s) 335, 368, 658, 727, 1082
Hpy166II GTNNAC 2 cut(s) 20, 134
Hpy188I TCNGA 6 cut(s) 126, 460, 634, 885, 1037, 1308
Hpy188III TCNNGA 6 cut(s) 84, 203, 242, 787, 1111, 1398
Hpy8I GTNNAC 2 cut(s) 20, 134
Hpy99I CGWCG 6 cut(s) 533, 584, 587, 647, 1022, 1025
HpyAV CCTTC 6 cut(s) 245, 487, 639, 841, 1108, 1273
HpyCH4III ACNGT 3 cut(s) 200, 580, 1322
HpyCH4IV ACGT 1 cut(s) 845
HpyCH4V TGCA 4 cut(s) 895, 1174, 1234, 1381
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 41, 219, 482, 933, 1387
HpyF3I CTNAG 2 cut(s) 218, 1287
HpySE526I ACGT 1 cut(s) 845
Hsp92I GRCGYC 3 cut(s) 531, 867, 1023
Hsp92II CATG 5 cut(s) 227, 652, 745, 1114, 1202
HspAI GCGC 5 cut(s) 483, 541, 751, 867, 1028
KasI GGCGCC 1 cut(s) 866
Kzo9I GATC 5 cut(s) 227, 723, 732, 789, 978
LmnI GCTCC 6 cut(s) 127, 548, 838, 1009, 1258, 1270
Lsp1109I GCAGC 1 cut(s) 1218
LweI GCATC 3 cut(s) 31, 235, 1290
MaeI CTAG 1 cut(s) 12
MaeII ACGT 1 cut(s) 845
MaeIII GTNAC 3 cut(s) 745, 846, 1070
MalI GATC 5 cut(s) 229, 725, 734, 791, 980
MbiI CCGCTC 1 cut(s) 698
MboI GATC 5 cut(s) 227, 723, 732, 789, 978
MboII GAAGA 6 cut(s) 362, 365, 432, 973, 988, 1177
MfeI CAATTG 1 cut(s) 1212
MflI RGATCY 2 cut(s) 789, 978
MhlI GDGCHC 3 cut(s) 501, 717, 843
MluCI AATT 2 cut(s) 916, 1212
Mly113I GGCGCC 1 cut(s) 867
MlyI GAGTC 1 cut(s) 889
MmeI TCCRAC 3 cut(s) 483, 639, 657
MroXI GAANNNNTTC 2 cut(s) 980, 1094
MseI TTAA 2 cut(s) 999, 1194
MslI CAYNNNNRTG 1 cut(s) 740
MspA1I CMGCKG 1 cut(s) 952
MspI CCGG 5 cut(s) 776, 928, 1007, 1068, 1268
MspR9I CCNGG 3 cut(s) 776, 827, 1008
MunI CAATTG 1 cut(s) 1212
Mva1269I GAATGC 1 cut(s) 1110
MvaI CCWGG 1 cut(s) 827
MvnI CGCG 4 cut(s) 483, 528, 751, 1028
MwoI GCNNNNNNNGC 6 cut(s) 32, 41, 219, 482, 933, 1387
NarI GGCGCC 1 cut(s) 867
NciI CCSGG 2 cut(s) 776, 1008
NcoI CCATGG 2 cut(s) 223, 648
NdeII GATC 5 cut(s) 227, 723, 732, 789, 978
NlaIII CATG 5 cut(s) 227, 652, 745, 1114, 1202
NlaIV GGNNCC 6 cut(s) 509, 868, 1015, 1260, 1272, 1284
NmuCI GTSAC 3 cut(s) 745, 846, 1070
PaeR7I CTCGAG 1 cut(s) 710
PagI TCATGA 1 cut(s) 1110
PceI AGGCCT 1 cut(s) 830
PctI GAATGC 1 cut(s) 1110
PdmI GAANNNNTTC 2 cut(s) 980, 1094
PfeI GAWTC 5 cut(s) 80, 158, 886, 904, 1168
PflMI CCANNNNNTGG 1 cut(s) 146
PfoI TCCNGGA 1 cut(s) 1006
PkrI GCNGC 9 cut(s) 212, 359, 454, 697, 803, 806, 866, 1027, 1233
PleI GAGTC 1 cut(s) 888
PluTI GGCGCC 1 cut(s) 870
PpsI GAGTC 1 cut(s) 888
PpuMI RGGWCCY 2 cut(s) 1177, 1282
PshAI GACNNNNGTC 2 cut(s) 581, 879
Psp124BI GAGCTC 1 cut(s) 843
Psp5II RGGWCCY 2 cut(s) 1177, 1282
Psp6I CCWGG 1 cut(s) 825
PspGI CCWGG 1 cut(s) 825
PspN4I GGNNCC 6 cut(s) 509, 868, 1015, 1260, 1272, 1284
PspPI GGNCC 4 cut(s) 507, 652, 1177, 1282
PspPPI RGGWCCY 2 cut(s) 1177, 1282
PspXI VCTCGAGB 1 cut(s) 710
PstI CTGCAG 1 cut(s) 1176
PstNI CAGNNNCTG 2 cut(s) 907, 1180
PsuI RGATCY 2 cut(s) 789, 978
PvuII CAGCTG 1 cut(s) 952
RsaI GTAC 1 cut(s) 932
RsaNI GTAC 1 cut(s) 931
RseI CAYNNNNRTG 1 cut(s) 740
SacI GAGCTC 1 cut(s) 843
SaqAI TTAA 2 cut(s) 999, 1194
SatI GCNGC 9 cut(s) 211, 358, 453, 696, 802, 805, 865, 1026, 1232
Sau3AI GATC 5 cut(s) 227, 723, 732, 789, 978
Sau96I GGNCC 4 cut(s) 507, 652, 1177, 1282
SchI GAGTC 1 cut(s) 889
ScrFI CCNGG 3 cut(s) 776, 827, 1008
SduI GDGCHC 3 cut(s) 501, 717, 843
SfaNI GCATC 3 cut(s) 31, 235, 1290
SfcI CTRYAG 1 cut(s) 1172
SfoI GGCGCC 1 cut(s) 868
Sfr274I CTCGAG 1 cut(s) 710
SinI GGWCC 4 cut(s) 507, 652, 1177, 1282
SlaI CTCGAG 1 cut(s) 710
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 2 cut(s) 710, 1354
SmoI CTYRAG 2 cut(s) 710, 1354
Sse9I AATT 2 cut(s) 916, 1212
SseBI AGGCCT 1 cut(s) 830
SspDI GGCGCC 1 cut(s) 866
SspMI CTAG 1 cut(s) 12
SstI GAGCTC 1 cut(s) 843
StuI AGGCCT 1 cut(s) 830
StyD4I CCNGG 3 cut(s) 774, 825, 1006
StyI CCWWGG 2 cut(s) 223, 648
TaaI ACNGT 3 cut(s) 200, 580, 1322
TaiI ACGT 1 cut(s) 848
TaqI TCGA 8 cut(s) 280, 513, 585, 711, 816, 834, 1098, 1397
TaqII GACCGA 1 cut(s) 1178
TasI AATT 2 cut(s) 916, 1212
TauI GCSGC 8 cut(s) 213, 360, 455, 698, 804, 807, 867, 1028
TfiI GAWTC 5 cut(s) 80, 158, 886, 904, 1168
Tru1I TTAA 2 cut(s) 999, 1194
Tru9I TTAA 2 cut(s) 999, 1194
TscAI CASTG 2 cut(s) 542, 1327
TseFI GTSAC 3 cut(s) 745, 846, 1070
TseI GCWGC 1 cut(s) 1231
Tsp45I GTSAC 3 cut(s) 745, 846, 1070
TspDTI ATGAA 4 cut(s) 45, 772, 1127, 1383
TspGWI ACGGA 3 cut(s) 53, 377, 457
TspRI CASTG 2 cut(s) 542, 1327
Van91I CCANNNNNTGG 1 cut(s) 146
VpaK11BI GGWCC 4 cut(s) 507, 652, 1177, 1282
XhoI CTCGAG 1 cut(s) 710
XmnI GAANNNNTTC 2 cut(s) 980, 1094
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.