Prupe.8G119100_v2.0.a1

nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0015047
Physical Location & Seq
Reverse (-)
14617812 .. 14618870
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G119100.1

Sequence Viewer

Length: 1059 bp
ATGTCCGTCTCCGCCTTCCGAGCCCTCGCCACCGAGCACATCTGGTCCCTCGAAGCCCCTCTCCGCGACGCCCAGTGGCGTTCCCTCTACGGCCTCTCCTATCCCGTCTTCACCACCGTCGTCGAGAAGCTCCGGCCTCACATCGCCCTCTCCAACCTCTCCCTCCCTTCCGACTACGCCGTCGCCATGGTCCTCTCCCGCCTCTCCCATGGCTTCTCCGCGCAAACCCTCGCCTCCCGCTACTCCCTCGACCCTTACCTCGTCTCCAAGATCACCAACATGGTCACCCGCCTCCTCGCCACCAAGCTCTACCCGGAGTTCATCAAGATCCCGGTCTCCCGCCGCAGACTCATCGAGACCACCCAGGCTTTCGAGGAGCTCACATCGCTTCCCAACATGTGTGGCGCCATTGACGGAAGCCCAATCAAGCTCCACAAGCACAACTTGCCTGGCAACTACAAATGCAAATATGGGTACCCTTCGGTTTTGCTCGAAGTTGTGGCTGATCACAAGAAAATCTTCTGGGATGTGTGTGTCAAAGCTCCAGGAGGCACAGACGACGCAACCCATTTTAGAGATAGTCTTTTGTATAATAGGCTGACTTCTGGTGATATTGTTTGGGATAAGGTGATCAATGTGAGGGGTCACCATGTGAGGCCTTACATTGTTGGGGACTGGTGCTATCCCTTGCTATCTTTTTTGTTGACACCCTTTTCCCCAAATGGGATGGGAACTCCTGCACAGAACCTGTTCGATGGAATGCTTATGAAGGGAAGGTCTGTTGTGGTTGAAGCAATTGGATTGCTGAAAGGGAGGTGGAAGATTCTGCAGGACCTCAATGTGGGTCTTAATCATGTACCCCAGACAATTGTTGCTTGTTGTGTGTTGCATAATTTGTGCCAGATTGCGAGAGAGCCTGAACCGGAGCTCTGGAAGGACCCTGATGAGAGTGGGGCGCCACCGAGGGTGCTTGACAGTGAGAAGTCGTTTTACTACTTTGGGGAAAGCTTGAGGCAAGCATTGGCTGATGATTTGCATCAGAGGCTTTCGTCGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

353

Amino Acids

39.61

Weight (kDa)

8.58

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19120
fragaria_vesca FvH4_2g06711
prunus_persica Prupe.8G119100_v2.0.a1
pyrus_communis pycom05g06910
rosa_chinensis RchiOBHm_Chr6g0258821
rosa_laevigata RLG00000014583
rosa_multiflora Rmu_co8359799.1_g000001 Rmu_sc0004485.1_g000014
rosa_roxburghii Rroxscaffold_7G00206090
rosa_rugosa Rorug05G0583500 Rorug05G0583600
rosa_samantha Rh6AG100100 Rh6BG091600 Rh6CG089100 Rh6DG083400
rosa_wichuraiana Rw6G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
Acc65I GGTACC 1 cut(s) 474
AccB1I GGYRCC 3 cut(s) 404, 474, 955
AccII CGCG 2 cut(s) 66, 221
AciI CCGC 8 cut(s) 12, 64, 199, 219, 238, 289, 340, 343
AclWI GGATC 1 cut(s) 322
AcyI GRCGYC 3 cut(s) 69, 405, 956
AdeI CACNNNGTG 1 cut(s) 652
AfaI GTAC 2 cut(s) 476, 858
AfiI CCNNNNNNNGG 2 cut(s) 723, 841
AflIII ACRYGT 1 cut(s) 396
AgsI TTSAA 1 cut(s) 791
AjnI CCWGG 3 cut(s) 363, 448, 544
AluBI AGCT 7 cut(s) 130, 307, 379, 430, 542, 928, 1008
AluI AGCT 7 cut(s) 130, 307, 379, 430, 542, 928, 1008
Alw21I GWGCWC 3 cut(s) 39, 381, 930
Alw26I GTCTC 4 cut(s) 13, 268, 340, 350
AlwI GGATC 1 cut(s) 322
AlwNI CAGNNNCTG 1 cut(s) 748
AoxI GGCC 3 cut(s) 91, 134, 656
Asp700I GAANNNNTTC 2 cut(s) 518, 749
Asp718I GGTACC 1 cut(s) 474
AspLEI GCGC 3 cut(s) 223, 407, 958
AspS9I GGNCC 4 cut(s) 45, 190, 832, 937
AsuC2I CCSGG 2 cut(s) 314, 332
AsuHPI GGTGA 6 cut(s) 103, 265, 277, 620, 638, 640
AvaII GGWCC 4 cut(s) 45, 190, 832, 937
BanI GGYRCC 3 cut(s) 404, 474, 955
BanII GRGCYC 3 cut(s) 25, 381, 930
BarI GAAGNNNNNNTAC 2 cut(s) 974, 1006
BbsI GAAGAC 1 cut(s) 100
Bbv12I GWGCWC 3 cut(s) 39, 381, 930
BccI CCATC 2 cut(s) 721, 749
BceAI ACGGC 2 cut(s) 106, 164
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BciT130I CCWGG 3 cut(s) 365, 450, 546
BclI TGATCA 2 cut(s) 505, 630
BcnI CCSGG 2 cut(s) 314, 332
BcoDI GTCTC 4 cut(s) 13, 268, 340, 350
BfmI CTRYAG 1 cut(s) 827
BfoI RGCGCY 2 cut(s) 408, 959
BisI GCNGC 1 cut(s) 343
BlsI GCNGC 1 cut(s) 344
Bme1390I CCNGG 5 cut(s) 314, 332, 365, 450, 546
Bme18I GGWCC 4 cut(s) 45, 190, 832, 937
BmgT120I GGNCC 4 cut(s) 45, 190, 832, 937
BmiI GGNNCC 5 cut(s) 47, 406, 476, 939, 957
BmrFI CCNGG 5 cut(s) 314, 332, 365, 450, 546
BmrI ACTGGG 1 cut(s) 67
BmsI GCATC 1 cut(s) 1045
BmuI ACTGGG 1 cut(s) 67
BpiI GAAGAC 1 cut(s) 100
BpmI CTGGAG 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 1030
BpuMI CCSGG 2 cut(s) 314, 332
BsaBI GATNNNNATC 1 cut(s) 1035
BsaHI GRCGYC 3 cut(s) 69, 405, 956
BsaI GGTCTC 2 cut(s) 340, 350
BsaJI CCNNGG 4 cut(s) 186, 208, 363, 962
BsaWI WCCGGW 1 cut(s) 922
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 248, 278
Bsc4I CCNNNNNNNGG 2 cut(s) 723, 841
Bse1I ACTGG 2 cut(s) 73, 680
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 3 cut(s) 365, 450, 546
BseDI CCNNGG 4 cut(s) 186, 208, 363, 962
BseGI GGATG 2 cut(s) 532, 732
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 2 cut(s) 723, 841
BseNI ACTGG 2 cut(s) 73, 680
BseRI GAGGAG 2 cut(s) 284, 389
BsgI GTGCAG 1 cut(s) 723
Bsh1236I CGCG 2 cut(s) 66, 221
BshFI GGCC 3 cut(s) 93, 136, 658
BshNI GGYRCC 3 cut(s) 404, 474, 955
BsiHKAI GWGCWC 3 cut(s) 39, 381, 930
BsiSI CCGG 4 cut(s) 133, 314, 332, 923
BslFI GGGAC 2 cut(s) 31, 686
BslI CCNNNNNNNGG 2 cut(s) 723, 841
BsmAI GTCTC 4 cut(s) 13, 268, 340, 350
BsmBI CGTCTC 2 cut(s) 13, 268
BsmFI GGGAC 2 cut(s) 31, 686
BsmI GAATGC 1 cut(s) 765
BsnI GGCC 3 cut(s) 93, 136, 658
Bso31I GGTCTC 2 cut(s) 340, 350
Bsp1286I GDGCHC 4 cut(s) 25, 39, 381, 930
Bsp143I GATC 4 cut(s) 270, 327, 505, 630
Bsp19I CCATGG 2 cut(s) 186, 208
BspACI CCGC 8 cut(s) 12, 64, 199, 219, 238, 289, 340, 343
BspANI GGCC 3 cut(s) 93, 136, 658
BspFNI CGCG 2 cut(s) 66, 221
BspLI GGNNCC 5 cut(s) 47, 406, 476, 939, 957
BspMAI CTGCAG 1 cut(s) 831
BspPI GGATC 1 cut(s) 322
BspT107I GGYRCC 3 cut(s) 404, 474, 955
BspTNI GGTCTC 2 cut(s) 340, 350
BsrI ACTGG 2 cut(s) 73, 680
BssECI CCNNGG 4 cut(s) 186, 208, 363, 962
BssMI GATC 4 cut(s) 270, 327, 505, 630
BssNI GRCGYC 3 cut(s) 69, 405, 956
BssT1I CCWWGG 2 cut(s) 186, 208
Bst2UI CCWGG 3 cut(s) 365, 450, 546
Bst4CI ACNGT 2 cut(s) 118, 977
BstACI GRCGYC 3 cut(s) 69, 405, 956
BstAPI GCANNNNNTGC 1 cut(s) 445
BstC8I GCNNGC 1 cut(s) 1017
BstDSI CCRYGG 2 cut(s) 186, 208
BstEII GGTNACC 2 cut(s) 283, 644
BstF5I GGATG 2 cut(s) 532, 732
BstFNI CGCG 2 cut(s) 66, 221
BstH2I RGCGCY 2 cut(s) 408, 959
BstHHI GCGC 3 cut(s) 223, 407, 958
BstKTI GATC 4 cut(s) 273, 330, 508, 633
BstMAI GTCTC 4 cut(s) 13, 268, 340, 350
BstMBI GATC 4 cut(s) 270, 327, 505, 630
BstMWI GCNNNNNNNGC 5 cut(s) 20, 385, 436, 445, 1042
BstNI CCWGG 3 cut(s) 365, 450, 546
BstNSI RCATGY 1 cut(s) 400
BstPI GGTNACC 2 cut(s) 283, 644
BstSCI CCNGG 5 cut(s) 312, 330, 363, 448, 544
BstSFI CTRYAG 1 cut(s) 827
BstUI CGCG 2 cut(s) 66, 221
BstV2I GAAGAC 1 cut(s) 100
BstX2I RGATCY 1 cut(s) 327
BstYI RGATCY 1 cut(s) 327
BsuRI GGCC 3 cut(s) 93, 136, 658
BtgI CCRYGG 2 cut(s) 186, 208
BtgZI GCGATG 2 cut(s) 127, 369
BtsCI GGATG 2 cut(s) 532, 732
BtsIMutI CAGTG 2 cut(s) 80, 982
Cac8I GCNNGC 1 cut(s) 1017
CaiI CAGNNNCTG 1 cut(s) 748
CfoI GCGC 3 cut(s) 223, 407, 958
Cfr13I GGNCC 4 cut(s) 45, 190, 832, 937
CseI GACGC 2 cut(s) 77, 569
Csp6I GTAC 2 cut(s) 475, 857
CspCI CAANNNNNGTGG 2 cut(s) 382, 417
CviAII CATG 6 cut(s) 187, 209, 280, 397, 650, 854
CviQI GTAC 2 cut(s) 475, 857
DinI GGCGCC 2 cut(s) 406, 957
DpnI GATC 4 cut(s) 272, 329, 507, 632
DpnII GATC 4 cut(s) 270, 327, 505, 630
DraIII CACNNNGTG 1 cut(s) 652
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
Ecl136II GAGCTC 2 cut(s) 379, 928
Eco130I CCWWGG 2 cut(s) 186, 208
Eco147I AGGCCT 1 cut(s) 658
Eco24I GRGCYC 3 cut(s) 25, 381, 930
Eco31I GGTCTC 2 cut(s) 340, 350
Eco47I GGWCC 4 cut(s) 45, 190, 832, 937
Eco53kI GAGCTC 2 cut(s) 379, 928
Eco91I GGTNACC 2 cut(s) 283, 644
EcoICRI GAGCTC 2 cut(s) 379, 928
EcoO109I RGGNCCY 2 cut(s) 832, 937
EcoO65I GGTNACC 2 cut(s) 283, 644
EcoRII CCWGG 3 cut(s) 363, 448, 544
EcoT14I CCWWGG 2 cut(s) 186, 208
EcoT38I GRGCYC 3 cut(s) 25, 381, 930
EgeI GGCGCC 2 cut(s) 406, 957
EheI GGCGCC 2 cut(s) 406, 957
ErhI CCWWGG 2 cut(s) 186, 208
Esp3I CGTCTC 2 cut(s) 13, 268
FaeI CATG 6 cut(s) 190, 212, 283, 400, 653, 857
FalI AAGNNNNNCTT 4 cut(s) 428, 460, 503, 535
FaqI GGGAC 2 cut(s) 31, 686
FatI CATG 6 cut(s) 186, 208, 279, 396, 649, 853
FauI CCCGC 4 cut(s) 206, 245, 296, 347
FbaI TGATCA 2 cut(s) 505, 630
Fnu4HI GCNGC 1 cut(s) 343
FokI GGATG 2 cut(s) 539, 739
FriOI GRGCYC 3 cut(s) 25, 381, 930
Fsp4HI GCNGC 1 cut(s) 343
GlaI GCGC 3 cut(s) 222, 406, 957
GluI GCNGC 1 cut(s) 343
GsuI CTGGAG 1 cut(s) 528
HaeII RGCGCY 2 cut(s) 408, 959
HaeIII GGCC 3 cut(s) 93, 136, 658
HapII CCGG 4 cut(s) 133, 314, 332, 923
HgaI GACGC 2 cut(s) 77, 569
HhaI GCGC 3 cut(s) 223, 407, 958
Hin1I GRCGYC 3 cut(s) 69, 405, 956
Hin1II CATG 6 cut(s) 190, 212, 283, 400, 653, 857
Hin6I GCGC 3 cut(s) 221, 405, 956
HinP1I GCGC 3 cut(s) 221, 405, 956
HincII GTYRAC 1 cut(s) 705
HindII GTYRAC 1 cut(s) 705
HindIII AAGCTT 1 cut(s) 1006
HinfI GANTC 2 cut(s) 348, 823
HpaII CCGG 4 cut(s) 133, 314, 332, 923
HphI GGTGA 6 cut(s) 103, 265, 277, 620, 638, 640
Hpy166II GTNNAC 1 cut(s) 705
Hpy188I TCNGA 3 cut(s) 20, 172, 1041
Hpy188III TCNNGA 5 cut(s) 124, 325, 355, 931, 1053
Hpy8I GTNNAC 1 cut(s) 705
Hpy99I CGWCG 6 cut(s) 71, 122, 125, 185, 563, 1054
HpyAV CCTTC 6 cut(s) 25, 177, 489, 763, 768, 928
HpyCH4III ACNGT 2 cut(s) 118, 977
HpyCH4V TGCA 5 cut(s) 465, 740, 829, 889, 1036
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 385, 436, 445, 1042
Hsp92I GRCGYC 3 cut(s) 69, 405, 956
Hsp92II CATG 6 cut(s) 190, 212, 283, 400, 653, 857
HspAI GCGC 3 cut(s) 221, 405, 956
KasI GGCGCC 2 cut(s) 404, 955
KpnI GGTACC 1 cut(s) 478
Ksp22I TGATCA 2 cut(s) 505, 630
Kzo9I GATC 4 cut(s) 270, 327, 505, 630
LmnI GCTCC 5 cut(s) 135, 376, 435, 547, 925
LweI GCATC 1 cut(s) 1045
MaeIII GTNAC 2 cut(s) 283, 644
MalI GATC 4 cut(s) 272, 329, 507, 632
MboI GATC 4 cut(s) 270, 327, 505, 630
MboII GAAGA 3 cut(s) 100, 511, 832
MfeI CAATTG 2 cut(s) 795, 867
MflI RGATCY 1 cut(s) 327
MhlI GDGCHC 4 cut(s) 25, 39, 381, 930
MluCI AATT 3 cut(s) 795, 867, 892
Mly113I GGCGCC 2 cut(s) 405, 956
MlyI GAGTC 1 cut(s) 342
MmeI TCCRAC 2 cut(s) 177, 195
MroXI GAANNNNTTC 2 cut(s) 518, 749
MseI TTAA 1 cut(s) 849
MslI CAYNNNNRTG 1 cut(s) 278
MspI CCGG 4 cut(s) 133, 314, 332, 923
MspR9I CCNGG 5 cut(s) 314, 332, 365, 450, 546
MunI CAATTG 2 cut(s) 795, 867
Mva1269I GAATGC 1 cut(s) 765
MvaI CCWGG 3 cut(s) 365, 450, 546
MvnI CGCG 2 cut(s) 66, 221
MwoI GCNNNNNNNGC 5 cut(s) 20, 385, 436, 445, 1042
NarI GGCGCC 2 cut(s) 405, 956
NciI CCSGG 2 cut(s) 314, 332
NcoI CCATGG 2 cut(s) 186, 208
NdeII GATC 4 cut(s) 270, 327, 505, 630
NlaIII CATG 6 cut(s) 190, 212, 283, 400, 653, 857
NlaIV GGNNCC 5 cut(s) 47, 406, 476, 939, 957
NmuCI GTSAC 2 cut(s) 283, 644
NspI RCATGY 1 cut(s) 400
PceI AGGCCT 1 cut(s) 658
PciI ACATGT 1 cut(s) 396
PctI GAATGC 1 cut(s) 765
PdmI GAANNNNTTC 2 cut(s) 518, 749
PfeI GAWTC 1 cut(s) 823
PfoI TCCNGGA 1 cut(s) 544
PkrI GCNGC 1 cut(s) 344
PleI GAGTC 1 cut(s) 342
PluTI GGCGCC 2 cut(s) 408, 959
PpsI GAGTC 1 cut(s) 342
PpuMI RGGWCCY 2 cut(s) 832, 937
PscI ACATGT 1 cut(s) 396
Psp124BI GAGCTC 2 cut(s) 381, 930
Psp5II RGGWCCY 2 cut(s) 832, 937
Psp6I CCWGG 3 cut(s) 363, 448, 544
PspEI GGTNACC 2 cut(s) 283, 644
PspGI CCWGG 3 cut(s) 363, 448, 544
PspN4I GGNNCC 5 cut(s) 47, 406, 476, 939, 957
PspPI GGNCC 4 cut(s) 45, 190, 832, 937
PspPPI RGGWCCY 2 cut(s) 832, 937
PstI CTGCAG 1 cut(s) 831
PstNI CAGNNNCTG 1 cut(s) 748
PsuI RGATCY 1 cut(s) 327
RsaI GTAC 2 cut(s) 476, 858
RsaNI GTAC 2 cut(s) 475, 857
RseI CAYNNNNRTG 1 cut(s) 278
SacI GAGCTC 2 cut(s) 381, 930
SaqAI TTAA 1 cut(s) 849
SatI GCNGC 1 cut(s) 343
Sau3AI GATC 4 cut(s) 270, 327, 505, 630
Sau96I GGNCC 4 cut(s) 45, 190, 832, 937
SchI GAGTC 1 cut(s) 342
ScrFI CCNGG 5 cut(s) 314, 332, 365, 450, 546
SduI GDGCHC 4 cut(s) 25, 39, 381, 930
SfaNI GCATC 1 cut(s) 1045
SfcI CTRYAG 1 cut(s) 827
SfoI GGCGCC 2 cut(s) 406, 957
SinI GGWCC 4 cut(s) 45, 190, 832, 937
SmiMI CAYNNNNRTG 1 cut(s) 278
SmlI CTYRAG 1 cut(s) 1009
SmoI CTYRAG 1 cut(s) 1009
Sse9I AATT 3 cut(s) 795, 867, 892
SseBI AGGCCT 1 cut(s) 658
SsiI CCGC 8 cut(s) 12, 64, 199, 219, 238, 289, 340, 343
SspDI GGCGCC 2 cut(s) 404, 955
SstI GAGCTC 2 cut(s) 381, 930
StuI AGGCCT 1 cut(s) 658
StyD4I CCNGG 5 cut(s) 312, 330, 363, 448, 544
StyI CCWWGG 2 cut(s) 186, 208
TaaI ACNGT 2 cut(s) 118, 977
TaqI TCGA 8 cut(s) 51, 123, 249, 354, 372, 492, 753, 1052
TasI AATT 3 cut(s) 795, 867, 892
TauI GCSGC 1 cut(s) 345
TfiI GAWTC 1 cut(s) 823
Tru1I TTAA 1 cut(s) 849
Tru9I TTAA 1 cut(s) 849
TscAI CASTG 2 cut(s) 80, 982
TseFI GTSAC 2 cut(s) 283, 644
Tsp45I GTSAC 2 cut(s) 283, 644
TspDTI ATGAA 2 cut(s) 310, 782
TspGWI ACGGA 1 cut(s) 429
TspRI CASTG 2 cut(s) 80, 982
VpaK11BI GGWCC 4 cut(s) 45, 190, 832, 937
XceI RCATGY 1 cut(s) 400
XmnI GAANNNNTTC 2 cut(s) 518, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.