Rw6G008660

nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015047
Physical Location & Seq
Reverse (-)
14667306 .. 14669289
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G008660.1

Sequence Viewer

Length: 1344 bp
ATGGATCAATCGTTCCTCCTGATGCTCTCCAACCTCCTCCACCTCCACAACTCCATCGACCCAACCACCTCCCTCATCTCCTCCCCCACCTCCACCACCACCGCCTCCTCCGCCTCCACCTCCACCTCCCCCACCTCCCTCCTCACCTCCTCCTCCGCCGCCCCTCTCCTCTTCTTCACCATCGCCTCCGTCCTCTCCTACATCGCCTCCTCCCGCCCTTCCTCCTCCTCCCCCTCCTCCCCCGCCGCCTCCTCCTCCTCCGCCGCCGCCGCCGCCGCCTCCGACTACTCCGTCTCGGCCTTCCGCGCCCTCTCCACCGAGCACATCTGGTCCCTCGAAGCCCCTCTCCGCGACGCCCAGTGGCGCTCCCTCTACGGCCTGTCGTATCCCGTCTTTACGACCGTCGTCGATAAGCTCAAACCCCACATTGCTCTCTCCAACCTCTCCCTCCCTTCCGACTACGCCGTCGCCATGGTCCTCTCCCGCCTCGCCCACGGCTTCTCCGCCAAGTCCCTCGCCGCCCGCTACTCCCTCGAGCCCTACCTCATCTCCAAAATCACCAACATGGTCACGCGCCTACTCGCCACCAAGCTCTACCCGGAGTTCATCAAGATCCCCGTCAGCCGCCGCAGACTGATCGAAACCACCCAGGCCTTCGAGGAGCTGACGTCACTCCCCAACATGTGTGGCGCCATTGATGGCAGTCCGATCAGGCTGCACAAGCAGAATCTGCCTGCGAATTACAAATGCAGGTACGGCTACAATGCTGTTCAGCTGGAGGTTGTTGCTGACCACAAGAAGATCTTCTGGGATGTTTGCGTTAAAGCGCCCGGAGGCACCGAAGACGCCACCCATTTTCGAGAGAGTTTGTTGTATAACAGGCTTACTTCCGGTGACATTGTGTGGGATAAGGTCATCAATGTGCGTGGTCACCCTGTGAGGCCTTACATTGTTGGGGACTGGTGCTATCCTTTGCTCAGATTCTTGTTGACACCGTTTTCGCCTAATGGGATGGGGACTCCTGCGCAGAACCTGTTCGATGGAATGCTCATGAAGGGGAGATCTGTGGTGGTTGAGGCAATTGGGCTGCTCAAGGGGAGGTGGAAGATTCTGCAGGACCTGAATGTGGGTCTTAATCATGTGCCGCAGACCATTGTTGCTTGCTGTGTGCTGCACAATTTGTGCCAGATTGCGAGGGAGCCTGAACCGGAGCTGTGGAAGGACCCTGAGGAGAATGGGATGCCTCCTAGGGTCCATGACAGTGAGAAGTCGTTGTACTACTTTGGGGAAAGCTTGAGGCAAGCATTGGCTGATGATTTGCACCAGAGGCTTTCATCGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

447

Amino Acids

49.22

Weight (kDa)

8.57

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_tran PF04827 219 - 394 9.1e-08 Plant transposon protein
DDE_Tnp_4 PF13359 246 - 393 5.5e-18 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19120
fragaria_vesca FvH4_2g06711
prunus_persica Prupe.8G119100_v2.0.a1
pyrus_communis pycom05g06910
rosa_chinensis RchiOBHm_Chr6g0258821
rosa_laevigata RLG00000014583
rosa_multiflora Rmu_co8359799.1_g000001 Rmu_sc0004485.1_g000014
rosa_roxburghii Rroxscaffold_7G00206090
rosa_rugosa Rorug05G0583500 Rorug05G0583600
rosa_samantha Rh6AG100100 Rh6BG091600 Rh6CG089100 Rh6DG083400
rosa_wichuraiana Rw6G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 290, 464
AatII GACGTC 1 cut(s) 671
Acc16I TGCGCA 1 cut(s) 1026
Acc36I ACCTGC 1 cut(s) 741
AccB1I GGYRCC 2 cut(s) 689, 836
AccII CGCG 3 cut(s) 306, 351, 574
AclWI GGATC 2 cut(s) 12, 607
AcyI GRCGYC 4 cut(s) 354, 668, 690, 846
AdeI CACNNNGTG 1 cut(s) 937
AfaI GTAC 2 cut(s) 755, 1277
AfiI CCNNNNNNNGG 1 cut(s) 1126
AflIII ACRYGT 1 cut(s) 681
AjnI CCWGG 1 cut(s) 648
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 6 cut(s) 415, 592, 664, 775, 1213, 1293
AluI AGCT 6 cut(s) 415, 592, 664, 775, 1213, 1293
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 1 cut(s) 298
AlwI GGATC 2 cut(s) 12, 607
AlwNI CAGNNNCTG 3 cut(s) 730, 1033, 1120
Ama87I CYCGRG 1 cut(s) 533
AoxI GGCC 4 cut(s) 297, 376, 651, 941
ApeKI GCWGC 3 cut(s) 715, 1087, 1171
ArsI GACNNNNNNTTYG 2 cut(s) 982, 1014
Asp700I GAANNNNTTC 2 cut(s) 803, 1034
AspA2I CCTAGG 1 cut(s) 1247
AspLEI GCGC 6 cut(s) 308, 366, 576, 692, 829, 1027
AspS9I GGNCC 5 cut(s) 330, 475, 1117, 1222, 1252
AsuC2I CCSGG 2 cut(s) 599, 831
AsuHPI GGTGA 5 cut(s) 136, 169, 550, 905, 923
AvaI CYCGRG 1 cut(s) 533
AvaII GGWCC 5 cut(s) 330, 475, 1117, 1222, 1252
AvrII CCTAGG 1 cut(s) 1247
AxyI CCTNAGG 1 cut(s) 1227
BanI GGYRCC 2 cut(s) 689, 836
BanII GRGCYC 1 cut(s) 540
BarI GAAGNNNNNNTAC 2 cut(s) 1259, 1291
BbsI GAAGAC 1 cut(s) 849
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 3 cut(s) 702, 1074, 1158
BccI CCATC 5 cut(s) 62, 188, 692, 1006, 1034
BceAI ACGGC 4 cut(s) 391, 449, 511, 772
BcgI CGANNNNNNTGC 4 cut(s) 619, 653, 697, 731
BciT130I CCWGG 1 cut(s) 650
BciVI GTATCC 1 cut(s) 396
BcnI CCSGG 2 cut(s) 599, 831
BcoDI GTCTC 1 cut(s) 298
BfaI CTAG 1 cut(s) 1248
BfmI CTRYAG 1 cut(s) 1112
BfoI RGCGCY 3 cut(s) 367, 693, 830
BfuAI ACCTGC 1 cut(s) 741
BfuI GTATCC 1 cut(s) 396
BglII AGATCT 2 cut(s) 801, 1061
BlnI CCTAGG 1 cut(s) 1247
Bme1390I CCNGG 3 cut(s) 599, 650, 831
Bme18I GGWCC 5 cut(s) 330, 475, 1117, 1222, 1252
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 5 cut(s) 330, 475, 1117, 1222, 1252
BmiI GGNNCC 6 cut(s) 332, 691, 838, 1200, 1224, 1253
BmrFI CCNGG 3 cut(s) 599, 650, 831
BmrI ACTGGG 1 cut(s) 352
BmsI GCATC 2 cut(s) 12, 1230
BmuI ACTGGG 1 cut(s) 352
BoxI GACNNNNGTC 1 cut(s) 404
BpiI GAAGAC 1 cut(s) 849
BpmI CTGGAG 1 cut(s) 797
BpuEI CTTGAG 2 cut(s) 1076, 1315
BpuMI CCSGG 2 cut(s) 599, 831
BsaHI GRCGYC 4 cut(s) 354, 668, 690, 846
BsaJI CCNNGG 4 cut(s) 471, 493, 648, 1247
BsaWI WCCGGW 2 cut(s) 890, 1207
BsaXI ACNNNNNCTCC 8 cut(s) 191, 221, 485, 515, 533, 563, 653, 683
Bsc4I CCNNNNNNNGG 1 cut(s) 1126
Bse1I ACTGG 2 cut(s) 358, 965
Bse21I CCTNAGG 1 cut(s) 1227
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 4 cut(s) 471, 493, 648, 1247
BseGI GGATG 3 cut(s) 817, 1017, 1245
BseLI CCNNNNNNNGG 1 cut(s) 1126
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 2 cut(s) 991, 1218
BseNI ACTGG 2 cut(s) 358, 965
BseXI GCAGC 3 cut(s) 702, 1074, 1158
BsgI GTGCAG 2 cut(s) 701, 1157
Bsh1236I CGCG 3 cut(s) 306, 351, 574
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 4 cut(s) 299, 378, 653, 943
BshNI GGYRCC 2 cut(s) 689, 836
BsiEI CGRYCG 1 cut(s) 402
BsiHKAI GWGCWC 1 cut(s) 324
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 4 cut(s) 599, 831, 891, 1208
BslFI GGGAC 4 cut(s) 316, 496, 971, 1030
BslI CCNNNNNNNGG 1 cut(s) 1126
BsmAI GTCTC 1 cut(s) 298
BsmBI CGTCTC 1 cut(s) 298
BsmFI GGGAC 4 cut(s) 316, 496, 971, 1030
BsmI GAATGC 1 cut(s) 1050
BsnI GGCC 4 cut(s) 299, 378, 653, 943
BsoBI CYCGRG 1 cut(s) 533
Bsp1286I GDGCHC 2 cut(s) 324, 540
Bsp143I GATC 6 cut(s) 4, 612, 636, 708, 801, 1061
Bsp19I CCATGG 1 cut(s) 471
BspANI GGCC 4 cut(s) 299, 378, 653, 943
BspCNI CTCAG 2 cut(s) 990, 1219
BspFNI CGCG 3 cut(s) 306, 351, 574
BspHI TCATGA 1 cut(s) 1050
BspLI GGNNCC 6 cut(s) 332, 691, 838, 1200, 1224, 1253
BspMAI CTGCAG 1 cut(s) 1116
BspMI ACCTGC 1 cut(s) 741
BspPI GGATC 2 cut(s) 12, 607
BspT107I GGYRCC 2 cut(s) 689, 836
BsrDI GCAATG 1 cut(s) 426
BsrI ACTGG 2 cut(s) 358, 965
BssECI CCNNGG 4 cut(s) 471, 493, 648, 1247
BssMI GATC 6 cut(s) 4, 612, 636, 708, 801, 1061
BssNI GRCGYC 4 cut(s) 354, 668, 690, 846
BssT1I CCWWGG 2 cut(s) 471, 1247
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 3 cut(s) 403, 996, 1262
Bst6I CTCTTC 1 cut(s) 176
BstACI GRCGYC 4 cut(s) 354, 668, 690, 846
BstAPI GCANNNNNTGC 1 cut(s) 730
BstC8I GCNNGC 4 cut(s) 523, 735, 1162, 1302
BstDEI CTNAG 2 cut(s) 977, 1227
BstDSI CCRYGG 2 cut(s) 471, 493
BstEII GGTNACC 1 cut(s) 929
BstF5I GGATG 3 cut(s) 817, 1017, 1245
BstFNI CGCG 3 cut(s) 306, 351, 574
BstH2I RGCGCY 3 cut(s) 367, 693, 830
BstHHI GCGC 6 cut(s) 308, 366, 576, 692, 829, 1027
BstKTI GATC 6 cut(s) 7, 615, 639, 711, 804, 1064
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 6 cut(s) 4, 612, 636, 708, 801, 1061
BstMCI CGRYCG 1 cut(s) 402
BstMWI GCNNNNNNNGC 9 cut(s) 110, 269, 272, 275, 305, 721, 730, 756, 1327
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 1 cut(s) 685
BstPAI GACNNNNGTC 1 cut(s) 404
BstPI GGTNACC 1 cut(s) 929
BstSCI CCNGG 3 cut(s) 597, 648, 829
BstSFI CTRYAG 1 cut(s) 1112
BstUI CGCG 3 cut(s) 306, 351, 574
BstV1I GCAGC 3 cut(s) 702, 1074, 1158
BstV2I GAAGAC 1 cut(s) 849
BstX2I RGATCY 3 cut(s) 612, 801, 1061
BstYI RGATCY 3 cut(s) 612, 801, 1061
Bsu36I CCTNAGG 1 cut(s) 1227
BsuI GTATCC 1 cut(s) 396
BsuRI GGCC 4 cut(s) 299, 378, 653, 943
BtgI CCRYGG 2 cut(s) 471, 493
BtgZI GCGATG 2 cut(s) 166, 187
BtsCI GGATG 3 cut(s) 817, 1017, 1245
BtsIMutI CAGTG 2 cut(s) 365, 1267
BveI ACCTGC 1 cut(s) 741
Cac8I GCNNGC 4 cut(s) 523, 735, 1162, 1302
CaiI CAGNNNCTG 3 cut(s) 730, 1033, 1120
CciI TCATGA 1 cut(s) 1050
CfoI GCGC 6 cut(s) 308, 366, 576, 692, 829, 1027
Cfr13I GGNCC 5 cut(s) 330, 475, 1117, 1222, 1252
CseI GACGC 2 cut(s) 362, 854
Csp6I GTAC 2 cut(s) 754, 1276
CspCI CAANNNNNGTGG 4 cut(s) 667, 702, 907, 942
CviAII CATG 6 cut(s) 472, 565, 682, 1051, 1139, 1256
CviQI GTAC 2 cut(s) 754, 1276
DdeI CTNAG 2 cut(s) 977, 1227
DinI GGCGCC 1 cut(s) 691
DpnI GATC 6 cut(s) 6, 614, 638, 710, 803, 1063
DpnII GATC 6 cut(s) 4, 612, 636, 708, 801, 1061
DraIII CACNNNGTG 1 cut(s) 937
DrdI GACNNNNNNGTC 2 cut(s) 290, 464
DseDI GACNNNNNNGTC 2 cut(s) 290, 464
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
EciI GGCGGA 4 cut(s) 100, 145, 250, 493
Eco130I CCWWGG 2 cut(s) 471, 1247
Eco147I AGGCCT 2 cut(s) 653, 943
Eco24I GRGCYC 1 cut(s) 540
Eco47I GGWCC 5 cut(s) 330, 475, 1117, 1222, 1252
Eco81I CCTNAGG 1 cut(s) 1227
Eco88I CYCGRG 1 cut(s) 533
Eco91I GGTNACC 1 cut(s) 929
EcoO109I RGGNCCY 2 cut(s) 1117, 1222
EcoO65I GGTNACC 1 cut(s) 929
EcoRII CCWGG 1 cut(s) 648
EcoT14I CCWWGG 2 cut(s) 471, 1247
EcoT38I GRGCYC 1 cut(s) 540
EgeI GGCGCC 1 cut(s) 691
EheI GGCGCC 1 cut(s) 691
ErhI CCWWGG 2 cut(s) 471, 1247
Esp3I CGTCTC 1 cut(s) 298
FaeI CATG 6 cut(s) 475, 568, 685, 1054, 1142, 1259
FaiI YATR 7 cut(s) 473, 566, 683, 876, 1052, 1140, 1257
FalI AAGNNNNNCTT 2 cut(s) 788, 820
FaqI GGGAC 4 cut(s) 316, 496, 971, 1030
FatI CATG 6 cut(s) 471, 564, 681, 1050, 1138, 1255
FauI CCCGC 4 cut(s) 221, 250, 491, 530
FokI GGATG 3 cut(s) 824, 1024, 1252
FriOI GRGCYC 1 cut(s) 540
FspBI CTAG 1 cut(s) 1248
FspI TGCGCA 1 cut(s) 1026
GlaI GCGC 6 cut(s) 307, 365, 575, 691, 828, 1026
GsuI CTGGAG 1 cut(s) 797
HaeII RGCGCY 3 cut(s) 367, 693, 830
HaeIII GGCC 4 cut(s) 299, 378, 653, 943
HapII CCGG 4 cut(s) 599, 831, 891, 1208
HgaI GACGC 2 cut(s) 362, 854
HhaI GCGC 6 cut(s) 308, 366, 576, 692, 829, 1027
Hin1I GRCGYC 4 cut(s) 354, 668, 690, 846
Hin1II CATG 6 cut(s) 475, 568, 685, 1054, 1142, 1259
Hin6I GCGC 6 cut(s) 306, 364, 574, 690, 827, 1025
HinP1I GCGC 6 cut(s) 306, 364, 574, 690, 827, 1025
HincII GTYRAC 1 cut(s) 990
HindII GTYRAC 1 cut(s) 990
HindIII AAGCTT 1 cut(s) 1291
HinfI GANTC 4 cut(s) 727, 981, 1018, 1108
HpaII CCGG 4 cut(s) 599, 831, 891, 1208
HphI GGTGA 5 cut(s) 136, 169, 550, 905, 923
Hpy166II GTNNAC 1 cut(s) 990
Hpy188I TCNGA 4 cut(s) 283, 457, 708, 980
Hpy188III TCNNGA 5 cut(s) 19, 610, 860, 1051, 1338
Hpy8I GTNNAC 1 cut(s) 990
Hpy99I CGWCG 4 cut(s) 356, 407, 410, 470
HpyAV CCTTC 6 cut(s) 228, 310, 462, 664, 1048, 1213
HpyCH4III ACNGT 3 cut(s) 403, 996, 1262
HpyCH4IV ACGT 1 cut(s) 668
HpyCH4V TGCA 5 cut(s) 718, 750, 1114, 1174, 1321
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 269, 272, 275, 305, 721, 730, 756, 1327
HpyF3I CTNAG 2 cut(s) 977, 1227
HpySE526I ACGT 1 cut(s) 668
Hsp92I GRCGYC 4 cut(s) 354, 668, 690, 846
Hsp92II CATG 6 cut(s) 475, 568, 685, 1054, 1142, 1259
HspAI GCGC 6 cut(s) 306, 364, 574, 690, 827, 1025
KasI GGCGCC 1 cut(s) 689
Kzo9I GATC 6 cut(s) 4, 612, 636, 708, 801, 1061
LmnI GCTCC 4 cut(s) 371, 661, 1198, 1210
Lsp1109I GCAGC 3 cut(s) 702, 1074, 1158
LweI GCATC 2 cut(s) 12, 1230
MaeI CTAG 1 cut(s) 1248
MaeII ACGT 1 cut(s) 668
MaeIII GTNAC 4 cut(s) 568, 669, 893, 929
MalI GATC 6 cut(s) 6, 614, 638, 710, 803, 1063
MboI GATC 6 cut(s) 4, 612, 636, 708, 801, 1061
MboII GAAGA 6 cut(s) 163, 166, 796, 811, 854, 1117
MfeI CAATTG 1 cut(s) 1080
MflI RGATCY 3 cut(s) 612, 801, 1061
MhlI GDGCHC 2 cut(s) 324, 540
MluCI AATT 3 cut(s) 739, 1080, 1177
Mly113I GGCGCC 1 cut(s) 690
MlyI GAGTC 1 cut(s) 1012
MmeI TCCRAC 4 cut(s) 54, 306, 462, 480
MroXI GAANNNNTTC 2 cut(s) 803, 1034
MseI TTAA 2 cut(s) 822, 1134
MslI CAYNNNNRTG 3 cut(s) 563, 920, 1260
MspA1I CMGCKG 1 cut(s) 775
MspI CCGG 4 cut(s) 599, 831, 891, 1208
MspR9I CCNGG 3 cut(s) 599, 650, 831
MunI CAATTG 1 cut(s) 1080
Mva1269I GAATGC 1 cut(s) 1050
MvaI CCWGG 1 cut(s) 650
MvnI CGCG 3 cut(s) 306, 351, 574
MwoI GCNNNNNNNGC 9 cut(s) 110, 269, 272, 275, 305, 721, 730, 756, 1327
NarI GGCGCC 1 cut(s) 690
NciI CCSGG 2 cut(s) 599, 831
NcoI CCATGG 1 cut(s) 471
NdeII GATC 6 cut(s) 4, 612, 636, 708, 801, 1061
NlaIII CATG 6 cut(s) 475, 568, 685, 1054, 1142, 1259
NlaIV GGNNCC 6 cut(s) 332, 691, 838, 1200, 1224, 1253
NmeAIII GCCGAG 1 cut(s) 275
NmuCI GTSAC 4 cut(s) 568, 669, 893, 929
NsbI TGCGCA 1 cut(s) 1026
NspI RCATGY 1 cut(s) 685
PaeR7I CTCGAG 1 cut(s) 533
PagI TCATGA 1 cut(s) 1050
PceI AGGCCT 2 cut(s) 653, 943
PciI ACATGT 1 cut(s) 681
PctI GAATGC 1 cut(s) 1050
PdmI GAANNNNTTC 2 cut(s) 803, 1034
PfeI GAWTC 3 cut(s) 727, 981, 1108
PleI GAGTC 1 cut(s) 1012
PluTI GGCGCC 1 cut(s) 693
PpsI GAGTC 1 cut(s) 1012
PpuMI RGGWCCY 2 cut(s) 1117, 1222
PscI ACATGT 1 cut(s) 681
PshAI GACNNNNGTC 1 cut(s) 404
Psp5II RGGWCCY 2 cut(s) 1117, 1222
Psp6I CCWGG 1 cut(s) 648
PspEI GGTNACC 1 cut(s) 929
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 6 cut(s) 332, 691, 838, 1200, 1224, 1253
PspPI GGNCC 5 cut(s) 330, 475, 1117, 1222, 1252
PspPPI RGGWCCY 2 cut(s) 1117, 1222
PspXI VCTCGAGB 1 cut(s) 533
PstI CTGCAG 1 cut(s) 1116
PstNI CAGNNNCTG 3 cut(s) 730, 1033, 1120
PsuI RGATCY 3 cut(s) 612, 801, 1061
PvuII CAGCTG 1 cut(s) 775
RsaI GTAC 2 cut(s) 755, 1277
RsaNI GTAC 2 cut(s) 754, 1276
RseI CAYNNNNRTG 3 cut(s) 563, 920, 1260
SaqAI TTAA 2 cut(s) 822, 1134
Sau3AI GATC 6 cut(s) 4, 612, 636, 708, 801, 1061
Sau96I GGNCC 5 cut(s) 330, 475, 1117, 1222, 1252
SchI GAGTC 1 cut(s) 1012
ScrFI CCNGG 3 cut(s) 599, 650, 831
SduI GDGCHC 2 cut(s) 324, 540
SfaNI GCATC 2 cut(s) 12, 1230
SfcI CTRYAG 1 cut(s) 1112
SfoI GGCGCC 1 cut(s) 691
Sfr274I CTCGAG 1 cut(s) 533
SinI GGWCC 5 cut(s) 330, 475, 1117, 1222, 1252
SlaI CTCGAG 1 cut(s) 533
SmiMI CAYNNNNRTG 3 cut(s) 563, 920, 1260
SmlI CTYRAG 3 cut(s) 533, 1091, 1294
SmoI CTYRAG 3 cut(s) 533, 1091, 1294
Sse9I AATT 3 cut(s) 739, 1080, 1177
SseBI AGGCCT 2 cut(s) 653, 943
SspDI GGCGCC 1 cut(s) 689
SspMI CTAG 1 cut(s) 1248
StuI AGGCCT 2 cut(s) 653, 943
StyD4I CCNGG 3 cut(s) 597, 648, 829
StyI CCWWGG 2 cut(s) 471, 1247
TaaI ACNGT 3 cut(s) 403, 996, 1262
TaiI ACGT 1 cut(s) 671
TaqI TCGA 9 cut(s) 57, 336, 408, 534, 639, 657, 859, 1038, 1337
TasI AATT 3 cut(s) 739, 1080, 1177
TatI WGTACW 1 cut(s) 1275
TfiI GAWTC 3 cut(s) 727, 981, 1108
Tru1I TTAA 2 cut(s) 822, 1134
Tru9I TTAA 2 cut(s) 822, 1134
TscAI CASTG 2 cut(s) 365, 1267
TseFI GTSAC 4 cut(s) 568, 669, 893, 929
TseI GCWGC 3 cut(s) 715, 1087, 1171
Tsp45I GTSAC 4 cut(s) 568, 669, 893, 929
TspDTI ATGAA 3 cut(s) 595, 1067, 1323
TspGWI ACGGA 2 cut(s) 178, 280
TspRI CASTG 2 cut(s) 365, 1267
VpaK11BI GGWCC 5 cut(s) 330, 475, 1117, 1222, 1252
XceI RCATGY 1 cut(s) 685
XhoI CTCGAG 1 cut(s) 533
XmaJI CCTAGG 1 cut(s) 1247
XmnI GAANNNNTTC 2 cut(s) 803, 1034
XspI CTAG 1 cut(s) 1248
ZraI GACGTC 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.