RLG00000014583

nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015047
Physical Location & Seq
Forward (+)
55052498 .. 55054008
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014583

Sequence Viewer

Length: 1416 bp
ATGACTTGCCATTTTCAGTTATTAAAGTCGACACAACTGTCGTTTCAGTCCAGAGTCGACGCAGAGCCCATGGATCAATCCTTCCTCCTGATGCTCTCCAACCTCCTCCACCTCCACAACTCCATCGACCCAACCACCTCCCTCATCTCCTCCCCCACCTCCACCACAACCGCCTCTTCCGCCTCCACCTCCACCTCCCCCACCTCCCTCCTCACCTCCTCCTCCGCCGCCCCTCTCCTCTTCTTCACCATCGCCTCCGTCCTCTCCTACATCGCCTCCTCCCGCCCTTCCTCCTCCTCCTCCTCCCCTTCCACCCCCGCCGCCTCCTCCTCCTCCGCAGCCGCCGCCGCCTCCGACTACTCCGTCTCCGCCTTCCGCGCCCTCTCCACCGAGCACATCTGGTCCCTCGAAGCCCCTCTCCGCGACGCCCAGTGGCGCTCCCTATACGGCCTGTCGTATCCCGTCTTTACGACCGTCGTCGATAAGCTCAAACCCCACATTGCTCTCTCCAACCTCTCCCTCCCTTCCGACTACGCCGTCGCCATGGTCCTCTCCCGCCTCGCCCACGGCTTCTCCGCCAAGTCCCTCGCCGCCCGCTACTCCCTCGAGCCCTACCTTATCTCCAAAATCACCAACATGGTCACGCGCCTACTCGCCACCAAGCTCTACCCGGAGTTCATCAAGATCCCCGTCAGCCGCCGCAGACTGATCGAAACCACCCAGGCCTTCGAGGAGCTGACGTCACTCCCCAACATGTGTGGCGCCATTGATGGCAGTCCGATCAGGCTGCACAAGCAGAATCTGCCTGGGAATTACAAGTGTAGGTACGGCTACAATGCTGTTCAGCTCGAGGTTGTTGCTGACCACAAGAAGATCTTCTGGGATGTTTGCGTTAAAGCGCCGGGAGGCACCGACGACGCCACCCATTTTCGAGAGAGTTTGTTGTATAACAGGCTTACTTCCGGTGACATTGTGTGGGATAAGGTCATCAGTGTGCGTGGTCACCATGTGAGGCCTTACATTGTTGGGGACTGGTGCTATCCTTTGCTCAGTTTCTTGTTGACTCCCTTTTCGCCTAATGGGATGGGGACTCCCGCGCAGAACCTGTTCGATGGAATGCTCATGAAGGGGAGGTCTGTGGTGGTTGAGGCAATTGGGCTGCTCAAAGGGAGGTGGAAGATTCTGCAGGACCTGAATGTGGGTCTTAATCATGTGCCGCAGACCATTGTTGCTTGCTGTGTGCTGCACAATTTGTGCCAGATTGCGAGGGAGCCTGAACCGGAGCTGTGGAAGGACCCCGAGGAGAATGGGATGCCTCCTAGGGTGCATGACAGTGAGAAGTCGTTTTACTACTTTGGGGAAAGCTTGAGGCAAGCATTGGCTGATGATTTGCACCAGAGGCTTTCATCGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

472

Amino Acids

51.93

Weight (kDa)

8.33

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_tran PF04827 243 - 418 3e-08 Plant transposon protein
DDE_Tnp_4 PF13359 271 - 417 8e-18 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19120
fragaria_vesca FvH4_2g06711
prunus_persica Prupe.8G119100_v2.0.a1
pyrus_communis pycom05g06910
rosa_chinensis RchiOBHm_Chr6g0258821
rosa_laevigata RLG00000014583
rosa_multiflora Rmu_co8359799.1_g000001 Rmu_sc0004485.1_g000014
rosa_roxburghii Rroxscaffold_7G00206090
rosa_rugosa Rorug05G0583500 Rorug05G0583600
rosa_samantha Rh6AG100100 Rh6BG091600 Rh6CG089100 Rh6DG083400
rosa_wichuraiana Rw6G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 37, 362, 536
AatII GACGTC 1 cut(s) 743
AccB1I GGYRCC 2 cut(s) 761, 908
AccI GTMKAC 2 cut(s) 29, 57
AccII CGCG 4 cut(s) 378, 423, 646, 1097
AclWI GGATC 2 cut(s) 81, 679
AcyI GRCGYC 4 cut(s) 426, 740, 762, 918
AdeI CACNNNGTG 1 cut(s) 1009
AfaI GTAC 1 cut(s) 827
AfiI CCNNNNNNNGG 1 cut(s) 1198
AflIII ACRYGT 1 cut(s) 753
AjnI CCWGG 2 cut(s) 720, 805
AluBI AGCT 6 cut(s) 487, 664, 736, 847, 1285, 1365
AluI AGCT 6 cut(s) 487, 664, 736, 847, 1285, 1365
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 370
AlwI GGATC 2 cut(s) 81, 679
AlwNI CAGNNNCTG 3 cut(s) 802, 1105, 1192
Ama87I CYCGRG 3 cut(s) 605, 848, 1298
AoxI GGCC 3 cut(s) 448, 723, 1013
ApeKI GCWGC 4 cut(s) 338, 787, 1159, 1243
ArsI GACNNNNNNTTYG 2 cut(s) 1054, 1086
Asp700I GAANNNNTTC 2 cut(s) 875, 1106
AspA2I CCTAGG 1 cut(s) 1319
AspLEI GCGC 6 cut(s) 380, 438, 648, 764, 901, 1099
AspS9I GGNCC 4 cut(s) 402, 547, 1189, 1294
AsuC2I CCSGG 2 cut(s) 671, 903
AsuHPI GGTGA 5 cut(s) 205, 238, 622, 977, 995
AvaI CYCGRG 3 cut(s) 605, 848, 1298
AvaII GGWCC 4 cut(s) 402, 547, 1189, 1294
AvrII CCTAGG 1 cut(s) 1319
BanI GGYRCC 2 cut(s) 761, 908
BanII GRGCYC 2 cut(s) 69, 612
BarI GAAGNNNNNNTAC 2 cut(s) 1331, 1363
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 4 cut(s) 350, 774, 1146, 1230
BccI CCATC 5 cut(s) 131, 257, 764, 1078, 1106
BceAI ACGGC 4 cut(s) 463, 521, 583, 844
BcgI CGANNNNNNTGC 6 cut(s) 691, 725, 769, 803, 839, 873
BciT130I CCWGG 2 cut(s) 722, 807
BciVI GTATCC 1 cut(s) 468
BcnI CCSGG 2 cut(s) 671, 903
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 1 cut(s) 1320
BfmI CTRYAG 1 cut(s) 1184
BfoI RGCGCY 3 cut(s) 439, 765, 902
BfuI GTATCC 1 cut(s) 468
BglII AGATCT 1 cut(s) 873
BlnI CCTAGG 1 cut(s) 1319
Bme1390I CCNGG 4 cut(s) 671, 722, 807, 903
Bme18I GGWCC 4 cut(s) 402, 547, 1189, 1294
BmeT110I CYCGRG 3 cut(s) 605, 848, 1298
BmgT120I GGNCC 4 cut(s) 402, 547, 1189, 1294
BmiI GGNNCC 5 cut(s) 404, 763, 910, 1272, 1296
BmrFI CCNGG 4 cut(s) 671, 722, 807, 903
BmrI ACTGGG 1 cut(s) 424
BmsI GCATC 2 cut(s) 81, 1302
BmuI ACTGGG 1 cut(s) 424
BoxI GACNNNNGTC 1 cut(s) 476
BpuEI CTTGAG 1 cut(s) 1387
BpuMI CCSGG 2 cut(s) 671, 903
BsaHI GRCGYC 4 cut(s) 426, 740, 762, 918
BsaJI CCNNGG 7 cut(s) 69, 543, 565, 720, 806, 1299, 1319
BsaWI WCCGGW 2 cut(s) 962, 1279
Bsc4I CCNNNNNNNGG 1 cut(s) 1198
Bse1I ACTGG 2 cut(s) 430, 1037
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 2 cut(s) 722, 807
BseDI CCNNGG 7 cut(s) 69, 543, 565, 720, 806, 1299, 1319
BseGI GGATG 3 cut(s) 889, 1089, 1317
BseLI CCNNNNNNNGG 1 cut(s) 1198
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 1063
BseNI ACTGG 2 cut(s) 430, 1037
BseXI GCAGC 4 cut(s) 350, 774, 1146, 1230
BsgI GTGCAG 2 cut(s) 773, 1229
Bsh1236I CGCG 4 cut(s) 378, 423, 646, 1097
Bsh1285I CGRYCG 1 cut(s) 474
BshFI GGCC 3 cut(s) 450, 725, 1015
BshNI GGYRCC 2 cut(s) 761, 908
BsiEI CGRYCG 1 cut(s) 474
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 3 cut(s) 605, 848, 1298
BsiSI CCGG 4 cut(s) 671, 902, 963, 1280
BslFI GGGAC 4 cut(s) 388, 568, 1043, 1102
BslI CCNNNNNNNGG 1 cut(s) 1198
BsmAI GTCTC 1 cut(s) 370
BsmBI CGTCTC 1 cut(s) 370
BsmFI GGGAC 4 cut(s) 388, 568, 1043, 1102
BsmI GAATGC 1 cut(s) 1122
BsnI GGCC 3 cut(s) 450, 725, 1015
BsoBI CYCGRG 3 cut(s) 605, 848, 1298
Bsp1286I GDGCHC 3 cut(s) 69, 396, 612
Bsp143I GATC 5 cut(s) 73, 684, 708, 780, 873
Bsp19I CCATGG 2 cut(s) 69, 543
BspANI GGCC 3 cut(s) 450, 725, 1015
BspCNI CTCAG 1 cut(s) 1062
BspFNI CGCG 4 cut(s) 378, 423, 646, 1097
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 5 cut(s) 404, 763, 910, 1272, 1296
BspMAI CTGCAG 1 cut(s) 1188
BspPI GGATC 2 cut(s) 81, 679
BspT107I GGYRCC 2 cut(s) 761, 908
BsrDI GCAATG 1 cut(s) 498
BsrI ACTGG 2 cut(s) 430, 1037
BssECI CCNNGG 7 cut(s) 69, 543, 565, 720, 806, 1299, 1319
BssMI GATC 5 cut(s) 73, 684, 708, 780, 873
BssNI GRCGYC 4 cut(s) 426, 740, 762, 918
BssT1I CCWWGG 3 cut(s) 69, 543, 1319
Bst2UI CCWGG 2 cut(s) 722, 807
Bst4CI ACNGT 3 cut(s) 39, 475, 1334
Bst6I CTCTTC 2 cut(s) 181, 245
BstACI GRCGYC 4 cut(s) 426, 740, 762, 918
BstAPI GCANNNNNTGC 1 cut(s) 802
BstC8I GCNNGC 3 cut(s) 595, 1234, 1374
BstDEI CTNAG 1 cut(s) 1049
BstDSI CCRYGG 3 cut(s) 69, 543, 565
BstEII GGTNACC 1 cut(s) 1001
BstF5I GGATG 3 cut(s) 889, 1089, 1317
BstFNI CGCG 4 cut(s) 378, 423, 646, 1097
BstH2I RGCGCY 3 cut(s) 439, 765, 902
BstHHI GCGC 6 cut(s) 380, 438, 648, 764, 901, 1099
BstKTI GATC 5 cut(s) 76, 687, 711, 783, 876
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 5 cut(s) 73, 684, 708, 780, 873
BstMCI CGRYCG 1 cut(s) 474
BstMWI GCNNNNNNNGC 7 cut(s) 179, 344, 347, 377, 793, 802, 1399
BstNI CCWGG 2 cut(s) 722, 807
BstNSI RCATGY 1 cut(s) 757
BstPAI GACNNNNGTC 1 cut(s) 476
BstPI GGTNACC 1 cut(s) 1001
BstSCI CCNGG 4 cut(s) 669, 720, 805, 901
BstSFI CTRYAG 1 cut(s) 1184
BstUI CGCG 4 cut(s) 378, 423, 646, 1097
BstV1I GCAGC 4 cut(s) 350, 774, 1146, 1230
BstX2I RGATCY 2 cut(s) 684, 873
BstYI RGATCY 2 cut(s) 684, 873
BsuI GTATCC 1 cut(s) 468
BsuRI GGCC 3 cut(s) 450, 725, 1015
BtgI CCRYGG 3 cut(s) 69, 543, 565
BtgZI GCGATG 2 cut(s) 235, 256
BtsCI GGATG 3 cut(s) 889, 1089, 1317
BtsIMutI CAGTG 3 cut(s) 437, 997, 1339
Cac8I GCNNGC 3 cut(s) 595, 1234, 1374
CaiI CAGNNNCTG 3 cut(s) 802, 1105, 1192
CciI TCATGA 1 cut(s) 1122
CfoI GCGC 6 cut(s) 380, 438, 648, 764, 901, 1099
Cfr13I GGNCC 4 cut(s) 402, 547, 1189, 1294
CseI GACGC 3 cut(s) 68, 434, 926
Csp6I GTAC 1 cut(s) 826
CspCI CAANNNNNGTGG 2 cut(s) 739, 774
CviAII CATG 8 cut(s) 70, 544, 637, 754, 1007, 1123, 1211, 1328
CviQI GTAC 1 cut(s) 826
DdeI CTNAG 1 cut(s) 1049
DinI GGCGCC 1 cut(s) 763
DpnI GATC 5 cut(s) 75, 686, 710, 782, 875
DpnII GATC 5 cut(s) 73, 684, 708, 780, 873
DraIII CACNNNGTG 1 cut(s) 1009
DrdI GACNNNNNNGTC 3 cut(s) 37, 362, 536
DseDI GACNNNNNNGTC 3 cut(s) 37, 362, 536
Eam1104I CTCTTC 2 cut(s) 181, 245
EarI CTCTTC 2 cut(s) 181, 245
EciI GGCGGA 4 cut(s) 169, 214, 358, 565
Eco130I CCWWGG 3 cut(s) 69, 543, 1319
Eco147I AGGCCT 2 cut(s) 725, 1015
Eco24I GRGCYC 2 cut(s) 69, 612
Eco47I GGWCC 4 cut(s) 402, 547, 1189, 1294
Eco88I CYCGRG 3 cut(s) 605, 848, 1298
Eco91I GGTNACC 1 cut(s) 1001
EcoO109I RGGNCCY 2 cut(s) 1189, 1294
EcoO65I GGTNACC 1 cut(s) 1001
EcoRII CCWGG 2 cut(s) 720, 805
EcoT14I CCWWGG 3 cut(s) 69, 543, 1319
EcoT38I GRGCYC 2 cut(s) 69, 612
EgeI GGCGCC 1 cut(s) 763
EheI GGCGCC 1 cut(s) 763
ErhI CCWWGG 3 cut(s) 69, 543, 1319
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 8 cut(s) 73, 547, 640, 757, 1010, 1126, 1214, 1331
FalI AAGNNNNNCTT 2 cut(s) 860, 892
FaqI GGGAC 4 cut(s) 388, 568, 1043, 1102
FatI CATG 8 cut(s) 69, 543, 636, 753, 1006, 1122, 1210, 1327
FauI CCCGC 5 cut(s) 290, 325, 563, 602, 1102
FblI GTMKAC 2 cut(s) 29, 57
FokI GGATG 3 cut(s) 896, 1096, 1324
FriOI GRGCYC 2 cut(s) 69, 612
FspBI CTAG 1 cut(s) 1320
GlaI GCGC 6 cut(s) 379, 437, 647, 763, 900, 1098
HaeII RGCGCY 3 cut(s) 439, 765, 902
HaeIII GGCC 3 cut(s) 450, 725, 1015
HapII CCGG 4 cut(s) 671, 902, 963, 1280
HgaI GACGC 3 cut(s) 68, 434, 926
HhaI GCGC 6 cut(s) 380, 438, 648, 764, 901, 1099
Hin1I GRCGYC 4 cut(s) 426, 740, 762, 918
Hin1II CATG 8 cut(s) 73, 547, 640, 757, 1010, 1126, 1214, 1331
Hin6I GCGC 6 cut(s) 378, 436, 646, 762, 899, 1097
HinP1I GCGC 6 cut(s) 378, 436, 646, 762, 899, 1097
HincII GTYRAC 3 cut(s) 30, 58, 1062
HindII GTYRAC 3 cut(s) 30, 58, 1062
HindIII AAGCTT 1 cut(s) 1363
HinfI GANTC 5 cut(s) 54, 799, 1063, 1090, 1180
HpaII CCGG 4 cut(s) 671, 902, 963, 1280
HphI GGTGA 5 cut(s) 205, 238, 622, 977, 995
Hpy166II GTNNAC 3 cut(s) 30, 58, 1062
Hpy188I TCNGA 3 cut(s) 355, 529, 780
Hpy188III TCNNGA 6 cut(s) 51, 88, 682, 932, 1123, 1410
Hpy8I GTNNAC 3 cut(s) 30, 58, 1062
Hpy99I CGWCG 7 cut(s) 62, 428, 479, 482, 542, 917, 920
HpyAV CCTTC 8 cut(s) 91, 297, 318, 382, 534, 736, 1120, 1285
HpyCH4III ACNGT 3 cut(s) 39, 475, 1334
HpyCH4IV ACGT 1 cut(s) 740
HpyCH4V TGCA 5 cut(s) 790, 1186, 1246, 1327, 1393
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 344, 347, 377, 793, 802, 1399
HpyF3I CTNAG 1 cut(s) 1049
HpySE526I ACGT 1 cut(s) 740
Hsp92I GRCGYC 4 cut(s) 426, 740, 762, 918
Hsp92II CATG 8 cut(s) 73, 547, 640, 757, 1010, 1126, 1214, 1331
HspAI GCGC 6 cut(s) 378, 436, 646, 762, 899, 1097
KasI GGCGCC 1 cut(s) 761
Kzo9I GATC 5 cut(s) 73, 684, 708, 780, 873
LmnI GCTCC 4 cut(s) 443, 733, 1270, 1282
Lsp1109I GCAGC 4 cut(s) 350, 774, 1146, 1230
LweI GCATC 2 cut(s) 81, 1302
MaeI CTAG 1 cut(s) 1320
MaeII ACGT 1 cut(s) 740
MaeIII GTNAC 4 cut(s) 640, 741, 965, 1001
MalI GATC 5 cut(s) 75, 686, 710, 782, 875
MboI GATC 5 cut(s) 73, 684, 708, 780, 873
MboII GAAGA 6 cut(s) 168, 232, 235, 868, 883, 1189
MfeI CAATTG 1 cut(s) 1152
MflI RGATCY 2 cut(s) 684, 873
MhlI GDGCHC 3 cut(s) 69, 396, 612
MluCI AATT 3 cut(s) 811, 1152, 1249
Mly113I GGCGCC 1 cut(s) 762
MlyI GAGTC 3 cut(s) 63, 1057, 1084
MmeI TCCRAC 4 cut(s) 123, 378, 534, 552
MroXI GAANNNNTTC 2 cut(s) 875, 1106
MseI TTAA 3 cut(s) 23, 894, 1206
MslI CAYNNNNRTG 3 cut(s) 635, 992, 1332
MspI CCGG 4 cut(s) 671, 902, 963, 1280
MspR9I CCNGG 4 cut(s) 671, 722, 807, 903
MunI CAATTG 1 cut(s) 1152
Mva1269I GAATGC 1 cut(s) 1122
MvaI CCWGG 2 cut(s) 722, 807
MvnI CGCG 4 cut(s) 378, 423, 646, 1097
MwoI GCNNNNNNNGC 7 cut(s) 179, 344, 347, 377, 793, 802, 1399
NarI GGCGCC 1 cut(s) 762
NciI CCSGG 2 cut(s) 671, 903
NcoI CCATGG 2 cut(s) 69, 543
NdeII GATC 5 cut(s) 73, 684, 708, 780, 873
NlaIII CATG 8 cut(s) 73, 547, 640, 757, 1010, 1126, 1214, 1331
NlaIV GGNNCC 5 cut(s) 404, 763, 910, 1272, 1296
NmuCI GTSAC 4 cut(s) 640, 741, 965, 1001
NspI RCATGY 1 cut(s) 757
PaeR7I CTCGAG 2 cut(s) 605, 848
PagI TCATGA 1 cut(s) 1122
PceI AGGCCT 2 cut(s) 725, 1015
PciI ACATGT 1 cut(s) 753
PctI GAATGC 1 cut(s) 1122
PdmI GAANNNNTTC 2 cut(s) 875, 1106
PfeI GAWTC 2 cut(s) 799, 1180
PleI GAGTC 3 cut(s) 62, 1057, 1084
PluTI GGCGCC 1 cut(s) 765
PpsI GAGTC 3 cut(s) 62, 1057, 1084
PpuMI RGGWCCY 2 cut(s) 1189, 1294
PscI ACATGT 1 cut(s) 753
PshAI GACNNNNGTC 1 cut(s) 476
Psp5II RGGWCCY 2 cut(s) 1189, 1294
Psp6I CCWGG 2 cut(s) 720, 805
PspEI GGTNACC 1 cut(s) 1001
PspGI CCWGG 2 cut(s) 720, 805
PspN4I GGNNCC 5 cut(s) 404, 763, 910, 1272, 1296
PspPI GGNCC 4 cut(s) 402, 547, 1189, 1294
PspPPI RGGWCCY 2 cut(s) 1189, 1294
PspXI VCTCGAGB 2 cut(s) 605, 848
PstI CTGCAG 1 cut(s) 1188
PstNI CAGNNNCTG 3 cut(s) 802, 1105, 1192
PsuI RGATCY 2 cut(s) 684, 873
RsaI GTAC 1 cut(s) 827
RsaNI GTAC 1 cut(s) 826
RseI CAYNNNNRTG 3 cut(s) 635, 992, 1332
SalI GTCGAC 2 cut(s) 28, 56
SaqAI TTAA 3 cut(s) 23, 894, 1206
Sau3AI GATC 5 cut(s) 73, 684, 708, 780, 873
Sau96I GGNCC 4 cut(s) 402, 547, 1189, 1294
SchI GAGTC 3 cut(s) 63, 1057, 1084
ScrFI CCNGG 4 cut(s) 671, 722, 807, 903
SduI GDGCHC 3 cut(s) 69, 396, 612
SfaNI GCATC 2 cut(s) 81, 1302
SfcI CTRYAG 1 cut(s) 1184
SfoI GGCGCC 1 cut(s) 763
Sfr274I CTCGAG 2 cut(s) 605, 848
SinI GGWCC 4 cut(s) 402, 547, 1189, 1294
SlaI CTCGAG 2 cut(s) 605, 848
SmiMI CAYNNNNRTG 3 cut(s) 635, 992, 1332
SmlI CTYRAG 3 cut(s) 605, 848, 1366
SmoI CTYRAG 3 cut(s) 605, 848, 1366
Sse9I AATT 3 cut(s) 811, 1152, 1249
SseBI AGGCCT 2 cut(s) 725, 1015
SspDI GGCGCC 1 cut(s) 761
SspMI CTAG 1 cut(s) 1320
StuI AGGCCT 2 cut(s) 725, 1015
StyD4I CCNGG 4 cut(s) 669, 720, 805, 901
StyI CCWWGG 3 cut(s) 69, 543, 1319
TaaI ACNGT 3 cut(s) 39, 475, 1334
TaiI ACGT 1 cut(s) 743
TasI AATT 3 cut(s) 811, 1152, 1249
TauI GCSGC 9 cut(s) 230, 323, 344, 347, 350, 593, 699, 702, 1219
TfiI GAWTC 2 cut(s) 799, 1180
Tru1I TTAA 3 cut(s) 23, 894, 1206
Tru9I TTAA 3 cut(s) 23, 894, 1206
TscAI CASTG 3 cut(s) 437, 997, 1339
TseFI GTSAC 4 cut(s) 640, 741, 965, 1001
TseI GCWGC 4 cut(s) 338, 787, 1159, 1243
Tsp45I GTSAC 4 cut(s) 640, 741, 965, 1001
TspDTI ATGAA 3 cut(s) 667, 1139, 1395
TspGWI ACGGA 2 cut(s) 247, 352
TspRI CASTG 3 cut(s) 437, 997, 1339
VpaK11BI GGWCC 4 cut(s) 402, 547, 1189, 1294
XceI RCATGY 1 cut(s) 757
XhoI CTCGAG 2 cut(s) 605, 848
XmaJI CCTAGG 1 cut(s) 1319
XmiI GTMKAC 2 cut(s) 29, 57
XmnI GAANNNNTTC 2 cut(s) 875, 1106
XspI CTAG 1 cut(s) 1320
ZraI GACGTC 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.