RchiOBHm_Chr6g0258821

nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015047
Physical Location & Seq
Reverse (-)
14175785 .. 14178731
2947 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23207

Sequence Viewer

Length: 1338 bp
ATGGATCAATCGTTCCTCCTGATGCTCTCCAACCTCCTCCACCTCCACAACTCCATCGACCCAACCACCTCCCTCATCTCCTCCCCCACCTCCACCACCACCGCCTCCTCCGCCTCCACCTCCACCTCCCCCACCTCCCTCCTCACCTCCTCCTCCGCCGCCCCTCTCCTCTTCTTCACCATCGCCTCCGTCCTCTCCTACATCGCCTCCTCCCGCCCTTCCTCCTCCTCCCCCTCCACCCCCGCCGCCTCCTCCTCCGCCGCCGCCGCCGCCTCCGACTACTCCGTCTCCGCCTTCCGCGCCCTCTCCACCGAGCACATCTGGTCCCTCGAAGCCCCTCTCCGCGACGCCCAGTGGCGCTCCCTCTACGGCCTGTCGTATCCCGTCTTCACGACCGTCGTCGATAAGCTCAAACCCCACATTGCTCTCTCCAACCTCTCCCTCCCTTCCGACTACGCCGTCGCCATGGTCCTCTCCCGCCTCGCCCACGGCTTCTCCGCCAAGTCCCTCGCCGCCCGCTACTCCCTCGAGCCCTACCTCATCTCCAAAATCACCAACATGGTCACGCGCCTACTCGCCACCAAGCTCTACCCGGAGTTCATCAAGATCCCCGTCAGCCGCCGCAGACTGATCGAAACCACCCAGGCCTTCGAGGAGCTGACGTCACTCCCCAACATGTGTGGCGCCATTGATGGCAGTCCGATCAGGCTGCACAAGCAGAATCTGCCCGGGAATTACAAGTGCAGGTACGGCTACAATGCTGTTCAGCTGGAGGTTGTTGCTGACCACAAGAAGATCTTCTGGGATGTTTGCGTTAAAGCGCCGGGAGGCACCGAAGACGCCACCCATTTTCGAGAGAGTTTGTTGTATAACAGGCTTACTTCCGGTGACATTGTGTGGGATAAGGTCATCAATGTGCGTGGTCACCCTGTGAGGCCTTACATTGTCGGGGACTGGTGCTATCCTTTGCTCAGTTTCTTGTTGACTCCGTTTTCGCCTAATGGGATGGGGACTCCTGCACAGAACCTGTTCGATGGAATGCTCATGAAGGGGAGGTCTGTGGTGGTTGAGGCAATTGGGCTGCTCAAAGGGAGGTGGAAGATTCTGCAGGACCTGAATGTGGGTCTTAATCATGTGCCGCAGACCATTGTTGCTTGCTGTGTGCTGCACAATTTGTGCCAGATTGCGAGGGAGCCTGAACCGGAGCTGTGGAAGGACCCTGAGGAGAATGGGATGCCTCCTAGGGTGCATGACAGTGAGAAGTCGTTGTACTACTTTGGGGAAAGCTTGAGGCAAGCATTGGCTGATGATTTGCACCAGAGGCTTTCATCGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

445

Amino Acids

48.99

Weight (kDa)

8.37

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_tran PF04827 217 - 392 5.7e-08 Plant transposon protein
DDE_Tnp_4 PF13359 245 - 391 7e-18 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19120
fragaria_vesca FvH4_2g06711
prunus_persica Prupe.8G119100_v2.0.a1
pyrus_communis pycom05g06910
rosa_chinensis RchiOBHm_Chr6g0258821
rosa_laevigata RLG00000014583
rosa_multiflora Rmu_co8359799.1_g000001 Rmu_sc0004485.1_g000014
rosa_roxburghii Rroxscaffold_7G00206090
rosa_rugosa Rorug05G0583500 Rorug05G0583600
rosa_samantha Rh6AG100100 Rh6BG091600 Rh6CG089100 Rh6DG083400
rosa_wichuraiana Rw6G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 284, 458
AatII GACGTC 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 735
AccB1I GGYRCC 2 cut(s) 683, 830
AccII CGCG 3 cut(s) 300, 345, 568
AclWI GGATC 2 cut(s) 12, 601
AcyI GRCGYC 4 cut(s) 348, 662, 684, 840
AdeI CACNNNGTG 1 cut(s) 931
AfaI GTAC 2 cut(s) 749, 1271
AfiI CCNNNNNNNGG 1 cut(s) 1120
AflIII ACRYGT 1 cut(s) 675
AjnI CCWGG 1 cut(s) 642
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 6 cut(s) 409, 586, 658, 769, 1207, 1287
AluI AGCT 6 cut(s) 409, 586, 658, 769, 1207, 1287
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 1 cut(s) 292
AlwI GGATC 2 cut(s) 12, 601
AlwNI CAGNNNCTG 3 cut(s) 724, 1027, 1114
Ama87I CYCGRG 2 cut(s) 527, 728
AoxI GGCC 3 cut(s) 370, 645, 935
ApeKI GCWGC 3 cut(s) 709, 1081, 1165
ArsI GACNNNNNNTTYG 2 cut(s) 976, 1008
Asp700I GAANNNNTTC 2 cut(s) 797, 1028
AspA2I CCTAGG 1 cut(s) 1241
AspLEI GCGC 5 cut(s) 302, 360, 570, 686, 823
AspS9I GGNCC 4 cut(s) 324, 469, 1111, 1216
AsuC2I CCSGG 4 cut(s) 593, 729, 730, 825
AsuHPI GGTGA 5 cut(s) 136, 169, 544, 899, 917
AvaI CYCGRG 2 cut(s) 527, 728
AvaII GGWCC 4 cut(s) 324, 469, 1111, 1216
AvrII CCTAGG 1 cut(s) 1241
AxyI CCTNAGG 1 cut(s) 1221
BanI GGYRCC 2 cut(s) 683, 830
BanII GRGCYC 1 cut(s) 534
BarI GAAGNNNNNNTAC 4 cut(s) 371, 403, 1253, 1285
BbsI GAAGAC 2 cut(s) 379, 843
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 3 cut(s) 696, 1068, 1152
BccI CCATC 5 cut(s) 62, 188, 686, 1000, 1028
BceAI ACGGC 4 cut(s) 385, 443, 505, 766
BcgI CGANNNNNNTGC 4 cut(s) 613, 647, 691, 725
BciT130I CCWGG 1 cut(s) 644
BciVI GTATCC 1 cut(s) 390
BcnI CCSGG 4 cut(s) 593, 729, 730, 825
BcoDI GTCTC 1 cut(s) 292
BfaI CTAG 1 cut(s) 1242
BfmI CTRYAG 1 cut(s) 1106
BfoI RGCGCY 3 cut(s) 361, 687, 824
BfuAI ACCTGC 1 cut(s) 735
BfuI GTATCC 1 cut(s) 390
BglII AGATCT 1 cut(s) 795
BlnI CCTAGG 1 cut(s) 1241
Bme1390I CCNGG 5 cut(s) 593, 644, 729, 730, 825
Bme18I GGWCC 4 cut(s) 324, 469, 1111, 1216
BmeT110I CYCGRG 2 cut(s) 527, 728
BmgT120I GGNCC 4 cut(s) 324, 469, 1111, 1216
BmiI GGNNCC 5 cut(s) 326, 685, 832, 1194, 1218
BmrFI CCNGG 5 cut(s) 593, 644, 729, 730, 825
BmrI ACTGGG 1 cut(s) 346
BmsI GCATC 2 cut(s) 12, 1224
BmuI ACTGGG 1 cut(s) 346
BoxI GACNNNNGTC 1 cut(s) 398
BpiI GAAGAC 2 cut(s) 379, 843
BpmI CTGGAG 1 cut(s) 791
BpuEI CTTGAG 1 cut(s) 1309
BpuMI CCSGG 4 cut(s) 593, 729, 730, 825
BsaHI GRCGYC 4 cut(s) 348, 662, 684, 840
BsaJI CCNNGG 5 cut(s) 465, 487, 642, 728, 1241
BsaWI WCCGGW 2 cut(s) 884, 1201
Bsc4I CCNNNNNNNGG 1 cut(s) 1120
Bse1I ACTGG 2 cut(s) 352, 959
Bse21I CCTNAGG 1 cut(s) 1221
Bse3DI GCAATG 1 cut(s) 420
BseBI CCWGG 1 cut(s) 644
BseDI CCNNGG 5 cut(s) 465, 487, 642, 728, 1241
BseGI GGATG 3 cut(s) 811, 1011, 1239
BseLI CCNNNNNNNGG 1 cut(s) 1120
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 2 cut(s) 985, 1212
BseNI ACTGG 2 cut(s) 352, 959
BseXI GCAGC 3 cut(s) 696, 1068, 1152
BsgI GTGCAG 4 cut(s) 695, 763, 1002, 1151
Bsh1236I CGCG 3 cut(s) 300, 345, 568
Bsh1285I CGRYCG 1 cut(s) 396
BshFI GGCC 3 cut(s) 372, 647, 937
BshNI GGYRCC 2 cut(s) 683, 830
BsiEI CGRYCG 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 318
BsiHKCI CYCGRG 2 cut(s) 527, 728
BsiSI CCGG 5 cut(s) 593, 729, 824, 885, 1202
BslFI GGGAC 4 cut(s) 310, 490, 965, 1024
BslI CCNNNNNNNGG 1 cut(s) 1120
BsmAI GTCTC 1 cut(s) 292
BsmBI CGTCTC 1 cut(s) 292
BsmFI GGGAC 4 cut(s) 310, 490, 965, 1024
BsmI GAATGC 1 cut(s) 1044
BsnI GGCC 3 cut(s) 372, 647, 937
BsoBI CYCGRG 2 cut(s) 527, 728
Bsp1286I GDGCHC 2 cut(s) 318, 534
Bsp143I GATC 5 cut(s) 4, 606, 630, 702, 795
Bsp19I CCATGG 1 cut(s) 465
BspANI GGCC 3 cut(s) 372, 647, 937
BspCNI CTCAG 2 cut(s) 984, 1213
BspFNI CGCG 3 cut(s) 300, 345, 568
BspHI TCATGA 1 cut(s) 1044
BspLI GGNNCC 5 cut(s) 326, 685, 832, 1194, 1218
BspMAI CTGCAG 1 cut(s) 1110
BspMI ACCTGC 1 cut(s) 735
BspPI GGATC 2 cut(s) 12, 601
BspT107I GGYRCC 2 cut(s) 683, 830
BsrDI GCAATG 1 cut(s) 420
BsrI ACTGG 2 cut(s) 352, 959
BssECI CCNNGG 5 cut(s) 465, 487, 642, 728, 1241
BssMI GATC 5 cut(s) 4, 606, 630, 702, 795
BssNI GRCGYC 4 cut(s) 348, 662, 684, 840
BssT1I CCWWGG 2 cut(s) 465, 1241
Bst2UI CCWGG 1 cut(s) 644
Bst4CI ACNGT 2 cut(s) 397, 1256
Bst6I CTCTTC 1 cut(s) 176
BstACI GRCGYC 4 cut(s) 348, 662, 684, 840
BstAPI GCANNNNNTGC 1 cut(s) 724
BstC8I GCNNGC 3 cut(s) 517, 1156, 1296
BstDEI CTNAG 2 cut(s) 971, 1221
BstDSI CCRYGG 2 cut(s) 465, 487
BstEII GGTNACC 1 cut(s) 923
BstF5I GGATG 3 cut(s) 811, 1011, 1239
BstFNI CGCG 3 cut(s) 300, 345, 568
BstH2I RGCGCY 3 cut(s) 361, 687, 824
BstHHI GCGC 5 cut(s) 302, 360, 570, 686, 823
BstKTI GATC 5 cut(s) 7, 609, 633, 705, 798
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 5 cut(s) 4, 606, 630, 702, 795
BstMCI CGRYCG 1 cut(s) 396
BstMWI GCNNNNNNNGC 8 cut(s) 110, 266, 269, 299, 715, 724, 750, 1321
BstNI CCWGG 1 cut(s) 644
BstNSI RCATGY 1 cut(s) 679
BstPAI GACNNNNGTC 1 cut(s) 398
BstPI GGTNACC 1 cut(s) 923
BstSCI CCNGG 5 cut(s) 591, 642, 727, 728, 823
BstSFI CTRYAG 1 cut(s) 1106
BstUI CGCG 3 cut(s) 300, 345, 568
BstV1I GCAGC 3 cut(s) 696, 1068, 1152
BstV2I GAAGAC 2 cut(s) 379, 843
BstX2I RGATCY 2 cut(s) 606, 795
BstYI RGATCY 2 cut(s) 606, 795
Bsu36I CCTNAGG 1 cut(s) 1221
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 3 cut(s) 372, 647, 937
BtgI CCRYGG 2 cut(s) 465, 487
BtgZI GCGATG 2 cut(s) 166, 187
BtsCI GGATG 3 cut(s) 811, 1011, 1239
BtsIMutI CAGTG 2 cut(s) 359, 1261
BveI ACCTGC 1 cut(s) 735
Cac8I GCNNGC 3 cut(s) 517, 1156, 1296
CaiI CAGNNNCTG 3 cut(s) 724, 1027, 1114
CciI TCATGA 1 cut(s) 1044
CfoI GCGC 5 cut(s) 302, 360, 570, 686, 823
Cfr13I GGNCC 4 cut(s) 324, 469, 1111, 1216
Cfr9I CCCGGG 1 cut(s) 728
CseI GACGC 2 cut(s) 356, 848
Csp6I GTAC 2 cut(s) 748, 1270
CspCI CAANNNNNGTGG 4 cut(s) 661, 696, 901, 936
CviAII CATG 6 cut(s) 466, 559, 676, 1045, 1133, 1250
CviQI GTAC 2 cut(s) 748, 1270
DdeI CTNAG 2 cut(s) 971, 1221
DinI GGCGCC 1 cut(s) 685
DpnI GATC 5 cut(s) 6, 608, 632, 704, 797
DpnII GATC 5 cut(s) 4, 606, 630, 702, 795
DraIII CACNNNGTG 1 cut(s) 931
DrdI GACNNNNNNGTC 2 cut(s) 284, 458
DseDI GACNNNNNNGTC 2 cut(s) 284, 458
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
EciI GGCGGA 5 cut(s) 100, 145, 247, 280, 487
Eco130I CCWWGG 2 cut(s) 465, 1241
Eco147I AGGCCT 2 cut(s) 647, 937
Eco24I GRGCYC 1 cut(s) 534
Eco47I GGWCC 4 cut(s) 324, 469, 1111, 1216
Eco81I CCTNAGG 1 cut(s) 1221
Eco88I CYCGRG 2 cut(s) 527, 728
Eco91I GGTNACC 1 cut(s) 923
EcoO109I RGGNCCY 2 cut(s) 1111, 1216
EcoO65I GGTNACC 1 cut(s) 923
EcoRII CCWGG 1 cut(s) 642
EcoT14I CCWWGG 2 cut(s) 465, 1241
EcoT38I GRGCYC 1 cut(s) 534
EgeI GGCGCC 1 cut(s) 685
EheI GGCGCC 1 cut(s) 685
ErhI CCWWGG 2 cut(s) 465, 1241
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 6 cut(s) 469, 562, 679, 1048, 1136, 1253
FaiI YATR 7 cut(s) 467, 560, 677, 870, 1046, 1134, 1251
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FaqI GGGAC 4 cut(s) 310, 490, 965, 1024
FatI CATG 6 cut(s) 465, 558, 675, 1044, 1132, 1249
FauI CCCGC 4 cut(s) 221, 250, 485, 524
FokI GGATG 3 cut(s) 818, 1018, 1246
FriOI GRGCYC 1 cut(s) 534
FspBI CTAG 1 cut(s) 1242
GlaI GCGC 5 cut(s) 301, 359, 569, 685, 822
GsuI CTGGAG 1 cut(s) 791
HaeII RGCGCY 3 cut(s) 361, 687, 824
HaeIII GGCC 3 cut(s) 372, 647, 937
HapII CCGG 5 cut(s) 593, 729, 824, 885, 1202
HgaI GACGC 2 cut(s) 356, 848
HhaI GCGC 5 cut(s) 302, 360, 570, 686, 823
Hin1I GRCGYC 4 cut(s) 348, 662, 684, 840
Hin1II CATG 6 cut(s) 469, 562, 679, 1048, 1136, 1253
Hin6I GCGC 5 cut(s) 300, 358, 568, 684, 821
HinP1I GCGC 5 cut(s) 300, 358, 568, 684, 821
HincII GTYRAC 1 cut(s) 984
HindII GTYRAC 1 cut(s) 984
HindIII AAGCTT 1 cut(s) 1285
HinfI GANTC 4 cut(s) 721, 985, 1012, 1102
HpaII CCGG 5 cut(s) 593, 729, 824, 885, 1202
HphI GGTGA 5 cut(s) 136, 169, 544, 899, 917
Hpy166II GTNNAC 1 cut(s) 984
Hpy188I TCNGA 3 cut(s) 277, 451, 702
Hpy188III TCNNGA 6 cut(s) 19, 391, 604, 854, 1045, 1332
Hpy8I GTNNAC 1 cut(s) 984
Hpy99I CGWCG 4 cut(s) 350, 401, 404, 464
HpyAV CCTTC 6 cut(s) 228, 304, 456, 658, 1042, 1207
HpyCH4III ACNGT 2 cut(s) 397, 1256
HpyCH4IV ACGT 1 cut(s) 662
HpyCH4V TGCA 7 cut(s) 712, 744, 1019, 1108, 1168, 1249, 1315
HpyF10VI GCNNNNNNNGC 8 cut(s) 110, 266, 269, 299, 715, 724, 750, 1321
HpyF3I CTNAG 2 cut(s) 971, 1221
HpySE526I ACGT 1 cut(s) 662
Hsp92I GRCGYC 4 cut(s) 348, 662, 684, 840
Hsp92II CATG 6 cut(s) 469, 562, 679, 1048, 1136, 1253
HspAI GCGC 5 cut(s) 300, 358, 568, 684, 821
KasI GGCGCC 1 cut(s) 683
Kzo9I GATC 5 cut(s) 4, 606, 630, 702, 795
LmnI GCTCC 4 cut(s) 365, 655, 1192, 1204
Lsp1109I GCAGC 3 cut(s) 696, 1068, 1152
LweI GCATC 2 cut(s) 12, 1224
MaeI CTAG 1 cut(s) 1242
MaeII ACGT 1 cut(s) 662
MaeIII GTNAC 4 cut(s) 562, 663, 887, 923
MalI GATC 5 cut(s) 6, 608, 632, 704, 797
MboI GATC 5 cut(s) 4, 606, 630, 702, 795
MboII GAAGA 7 cut(s) 163, 166, 379, 790, 805, 848, 1111
MfeI CAATTG 1 cut(s) 1074
MflI RGATCY 2 cut(s) 606, 795
MhlI GDGCHC 2 cut(s) 318, 534
MluCI AATT 3 cut(s) 733, 1074, 1171
Mly113I GGCGCC 1 cut(s) 684
MlyI GAGTC 2 cut(s) 979, 1006
MmeI TCCRAC 4 cut(s) 54, 300, 456, 474
MroXI GAANNNNTTC 2 cut(s) 797, 1028
MseI TTAA 2 cut(s) 816, 1128
MslI CAYNNNNRTG 3 cut(s) 557, 914, 1254
MspA1I CMGCKG 1 cut(s) 769
MspI CCGG 5 cut(s) 593, 729, 824, 885, 1202
MspR9I CCNGG 5 cut(s) 593, 644, 729, 730, 825
MunI CAATTG 1 cut(s) 1074
Mva1269I GAATGC 1 cut(s) 1044
MvaI CCWGG 1 cut(s) 644
MvnI CGCG 3 cut(s) 300, 345, 568
MwoI GCNNNNNNNGC 8 cut(s) 110, 266, 269, 299, 715, 724, 750, 1321
NarI GGCGCC 1 cut(s) 684
NciI CCSGG 4 cut(s) 593, 729, 730, 825
NcoI CCATGG 1 cut(s) 465
NdeII GATC 5 cut(s) 4, 606, 630, 702, 795
NlaIII CATG 6 cut(s) 469, 562, 679, 1048, 1136, 1253
NlaIV GGNNCC 5 cut(s) 326, 685, 832, 1194, 1218
NmuCI GTSAC 4 cut(s) 562, 663, 887, 923
NspI RCATGY 1 cut(s) 679
PaeR7I CTCGAG 1 cut(s) 527
PagI TCATGA 1 cut(s) 1044
PceI AGGCCT 2 cut(s) 647, 937
PciI ACATGT 1 cut(s) 675
PctI GAATGC 1 cut(s) 1044
PdmI GAANNNNTTC 2 cut(s) 797, 1028
PfeI GAWTC 2 cut(s) 721, 1102
PleI GAGTC 2 cut(s) 979, 1006
PluTI GGCGCC 1 cut(s) 687
PpsI GAGTC 2 cut(s) 979, 1006
PpuMI RGGWCCY 2 cut(s) 1111, 1216
PscI ACATGT 1 cut(s) 675
PshAI GACNNNNGTC 1 cut(s) 398
Psp5II RGGWCCY 2 cut(s) 1111, 1216
Psp6I CCWGG 1 cut(s) 642
PspEI GGTNACC 1 cut(s) 923
PspGI CCWGG 1 cut(s) 642
PspN4I GGNNCC 5 cut(s) 326, 685, 832, 1194, 1218
PspPI GGNCC 4 cut(s) 324, 469, 1111, 1216
PspPPI RGGWCCY 2 cut(s) 1111, 1216
PspXI VCTCGAGB 1 cut(s) 527
PstI CTGCAG 1 cut(s) 1110
PstNI CAGNNNCTG 3 cut(s) 724, 1027, 1114
PsuI RGATCY 2 cut(s) 606, 795
PvuII CAGCTG 1 cut(s) 769
RsaI GTAC 2 cut(s) 749, 1271
RsaNI GTAC 2 cut(s) 748, 1270
RseI CAYNNNNRTG 3 cut(s) 557, 914, 1254
SaqAI TTAA 2 cut(s) 816, 1128
Sau3AI GATC 5 cut(s) 4, 606, 630, 702, 795
Sau96I GGNCC 4 cut(s) 324, 469, 1111, 1216
SchI GAGTC 2 cut(s) 979, 1006
ScrFI CCNGG 5 cut(s) 593, 644, 729, 730, 825
SduI GDGCHC 2 cut(s) 318, 534
SfaNI GCATC 2 cut(s) 12, 1224
SfcI CTRYAG 1 cut(s) 1106
SfoI GGCGCC 1 cut(s) 685
Sfr274I CTCGAG 1 cut(s) 527
SinI GGWCC 4 cut(s) 324, 469, 1111, 1216
SlaI CTCGAG 1 cut(s) 527
SmaI CCCGGG 1 cut(s) 730
SmiMI CAYNNNNRTG 3 cut(s) 557, 914, 1254
SmlI CTYRAG 2 cut(s) 527, 1288
SmoI CTYRAG 2 cut(s) 527, 1288
Sse9I AATT 3 cut(s) 733, 1074, 1171
SseBI AGGCCT 2 cut(s) 647, 937
SspDI GGCGCC 1 cut(s) 683
SspMI CTAG 1 cut(s) 1242
StuI AGGCCT 2 cut(s) 647, 937
StyD4I CCNGG 5 cut(s) 591, 642, 727, 728, 823
StyI CCWWGG 2 cut(s) 465, 1241
TaaI ACNGT 2 cut(s) 397, 1256
TaiI ACGT 1 cut(s) 665
TaqI TCGA 9 cut(s) 57, 330, 402, 528, 633, 651, 853, 1032, 1331
TasI AATT 3 cut(s) 733, 1074, 1171
TatI WGTACW 1 cut(s) 1269
TfiI GAWTC 2 cut(s) 721, 1102
Tru1I TTAA 2 cut(s) 816, 1128
Tru9I TTAA 2 cut(s) 816, 1128
TscAI CASTG 2 cut(s) 359, 1261
TseFI GTSAC 4 cut(s) 562, 663, 887, 923
TseI GCWGC 3 cut(s) 709, 1081, 1165
Tsp45I GTSAC 4 cut(s) 562, 663, 887, 923
TspDTI ATGAA 3 cut(s) 589, 1061, 1317
TspGWI ACGGA 3 cut(s) 178, 274, 978
TspMI CCCGGG 1 cut(s) 728
TspRI CASTG 2 cut(s) 359, 1261
VpaK11BI GGWCC 4 cut(s) 324, 469, 1111, 1216
XceI RCATGY 1 cut(s) 679
XhoI CTCGAG 1 cut(s) 527
XmaI CCCGGG 1 cut(s) 728
XmaJI CCTAGG 1 cut(s) 1241
XmnI GAANNNNTTC 2 cut(s) 797, 1028
XspI CTAG 1 cut(s) 1242
ZraI GACGTC 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.