Rorug05G0583500

nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0015047
Physical Location & Seq
Reverse (-)
78052759 .. 78054415
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0583500.1

Sequence Viewer

Length: 1185 bp
ATGAATCAACTTGGCACTTTAGTTGCAGACTATATTTCTAATGGAGAGTGGACCTTCCCATCTTTACTTCAATTACACCTCCCAGGTACGCTTTCCGCACGTGAAGCTTATGATTTTCTAAGGCCCCGATATGCTGTTGCTAATTGGGGAAAATTAATTTGGACAAAGTTTATTCCTCCTCGTATGTCTTTACTAGTTTGGAAGGTGTTAAGTGGTCGTATATTATCGGATGACTTCCTTCAAAAGAGAGGGGTCTCTCTAGCACCTCGGTGTTATCTCTGTCACATTCAAGTGGAGTCTCTGGAGCATATTTTCCTCTCTTGCTCTTTTGCATCATCATTATGGCAACGCTTAGTGCATGTGTTTGAGCTCGGCATGGTTCAGGGTTCCCTTATGGAGAGGCTACAACGGGGCTTGGATCATAGTCGCAGTCCTCAACTACGTGAGCTATGGCTCACCGCTTTTGTGGCTTTGTTATGGTGCATTTGGCATTTTCGAAACCGGGCTAAGTTTGATGGTGTGCAGGCCAACAAGAGTAATGCCTGGAGATTAATTAAAGGGCAAGTTAGCTCATCCAATAGAATTGGTTCTGGTTGCATGTACAATTCTGTTCGAGATATTACTATTTTGAAGCAGTTCCCTGTCCCATGCAAGCCCCGTAGGACTCCACGTATTGTCGAGGTTAATTGGCATCCTCCACTTCTTGGTTGGGTAAAGATTAATACTGATGGTGCTTGGCGTAGCTCTTCTGGGCATGCGGGCTATGGTGGCATCTTTAGAGATTTCAGAGGTGGTATTATGGGTGCTTTTTGCTCTAATCTTCTTATTCCAAGTTCTGTGGCAGCGGAGATTATGGCTGTCATCAAGGCTATTGAGCTAGCTCGGGTCCGGGACTGGAAATTTATTTGGTTAGAAGTGGACTCGGCTCTTATCCTTTCATACCTTCATTCTCCTCACTTGGTACCTTGGCAGCTAAGAATTGAGTGGCATAATTGTTTGCATCGTATCTCTCAGATGCAATTTCGCTGTTCACACATCTATCGTGAGGGAAACCAAGCTGCAGATGCTCTTGCTAACATGGGTCTTTCTTCTACGGGTTTAACCTGGTGGGACATAGCCCCAGATCTTATTCGTTCTCATTGTCGGAAAGACTCCTTGGGGTTGCCTAATTTTCGTGTTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

394

Amino Acids

45.16

Weight (kDa)

9.8

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 32 - 115 6.1e-24 zinc-binding in reverse transcriptase
RVT_3 PF13456 241 - 360 9.4e-18 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19120
fragaria_vesca FvH4_2g06711
prunus_persica Prupe.8G119100_v2.0.a1
pyrus_communis pycom05g06910
rosa_chinensis RchiOBHm_Chr6g0258821
rosa_laevigata RLG00000014583
rosa_multiflora Rmu_co8359799.1_g000001 Rmu_sc0004485.1_g000014
rosa_roxburghii Rroxscaffold_7G00206090
rosa_rugosa Rorug05G0583500 Rorug05G0583600
rosa_samantha Rh6AG100100 Rh6BG091600 Rh6CG089100 Rh6DG083400
rosa_wichuraiana Rw6G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 961
AccB1I GGYRCC 1 cut(s) 961
AccB7I CCANNNNNTGG 1 cut(s) 704
AciI CCGC 4 cut(s) 96, 459, 758, 845
AclWI GGATC 1 cut(s) 426
AcsI RAATTY 1 cut(s) 899
AcvI CACGTG 1 cut(s) 101
AfaI GTAC 3 cut(s) 88, 602, 963
AfiI CCNNNNNNNGG 1 cut(s) 704
AgsI TTSAA 4 cut(s) 71, 242, 290, 631
AhlI ACTAGT 1 cut(s) 193
AjnI CCWGG 3 cut(s) 82, 542, 1103
AjuI GAANNNNNNNTTGG 4 cut(s) 142, 174, 1139, 1171
AleI CACNNNNGTG 1 cut(s) 268
AluBI AGCT 9 cut(s) 107, 370, 448, 570, 744, 877, 881, 973, 1058
AluI AGCT 9 cut(s) 107, 370, 448, 570, 744, 877, 881, 973, 1058
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 2 cut(s) 259, 303
AlwI GGATC 1 cut(s) 426
Ama87I CYCGRG 1 cut(s) 882
AoxI GGCC 2 cut(s) 122, 525
ApeKI GCWGC 3 cut(s) 842, 970, 1058
ApoI RAATTY 1 cut(s) 899
AseI ATTAAT 3 cut(s) 155, 551, 720
Asp700I GAANNNNTTC 2 cut(s) 586, 635
Asp718I GGTACC 1 cut(s) 961
AspS9I GGNCC 3 cut(s) 51, 123, 886
AsuC2I CCSGG 2 cut(s) 503, 890
AsuHPI GGTGA 1 cut(s) 448
AsuII TTCGAA 1 cut(s) 496
AsuNHI GCTAGC 1 cut(s) 877
AvaI CYCGRG 1 cut(s) 882
AvaII GGWCC 2 cut(s) 51, 886
BanI GGYRCC 1 cut(s) 961
BanII GRGCYC 1 cut(s) 372
BbrPI CACGTG 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 3 cut(s) 854, 982, 1045
BccI CCATC 3 cut(s) 67, 509, 722
BciT130I CCWGG 3 cut(s) 84, 544, 1105
BcnI CCSGG 2 cut(s) 503, 890
BcoDI GTCTC 2 cut(s) 259, 303
BcuI ACTAGT 1 cut(s) 193
BfaI CTAG 3 cut(s) 194, 260, 878
BfmI CTRYAG 1 cut(s) 1059
BglII AGATCT 1 cut(s) 1123
BisI GCNGC 3 cut(s) 843, 971, 1059
BlsI GCNGC 3 cut(s) 844, 972, 1060
Bme1390I CCNGG 5 cut(s) 84, 503, 544, 890, 1105
Bme18I GGWCC 2 cut(s) 51, 886
BmeT110I CYCGRG 1 cut(s) 882
BmgT120I GGNCC 3 cut(s) 51, 123, 886
BmiI GGNNCC 4 cut(s) 125, 388, 887, 963
BmrFI CCNGG 5 cut(s) 84, 503, 544, 890, 1105
BmsI GCATC 6 cut(s) 341, 700, 780, 1005, 1009, 1054
BmtI GCTAGC 1 cut(s) 881
BpmI CTGGAG 2 cut(s) 323, 565
Bpu14I TTCGAA 1 cut(s) 496
BpuMI CCSGG 2 cut(s) 503, 890
BsaAI YACGTR 3 cut(s) 101, 443, 671
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 4 cut(s) 82, 266, 965, 1155
Bsc4I CCNNNNNNNGG 1 cut(s) 704
Bse1I ACTGG 1 cut(s) 899
BseBI CCWGG 3 cut(s) 84, 544, 1105
BseDI CCNNGG 4 cut(s) 82, 266, 965, 1155
BseGI GGATG 3 cut(s) 235, 572, 691
BseLI CCNNNNNNNGG 1 cut(s) 704
BseMII CTCAG 1 cut(s) 1025
BseNI ACTGG 1 cut(s) 899
BseRI GAGGAG 2 cut(s) 168, 942
BseXI GCAGC 3 cut(s) 854, 982, 1045
BsgI GTGCAG 1 cut(s) 542
BshFI GGCC 2 cut(s) 124, 527
BshNI GGYRCC 1 cut(s) 961
BsiHKAI GWGCWC 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 882
BsiSI CCGG 2 cut(s) 502, 889
BslFI GGGAC 3 cut(s) 629, 905, 1124
BslI CCNNNNNNNGG 1 cut(s) 704
BsmAI GTCTC 2 cut(s) 259, 303
BsmFI GGGAC 3 cut(s) 629, 905, 1124
BsnI GGCC 2 cut(s) 124, 527
Bso31I GGTCTC 1 cut(s) 259
BsoBI CYCGRG 1 cut(s) 882
Bsp119I TTCGAA 1 cut(s) 496
Bsp1286I GDGCHC 1 cut(s) 372
Bsp1407I TGTACA 1 cut(s) 600
Bsp143I GATC 2 cut(s) 418, 1123
BspACI CCGC 4 cut(s) 96, 459, 758, 845
BspANI GGCC 2 cut(s) 124, 527
BspCNI CTCAG 1 cut(s) 1024
BspLI GGNNCC 4 cut(s) 125, 388, 887, 963
BspMAI CTGCAG 1 cut(s) 1063
BspOI GCTAGC 1 cut(s) 881
BspPI GGATC 1 cut(s) 426
BspQI GCTCTTC 1 cut(s) 751
BspT104I TTCGAA 1 cut(s) 496
BspT107I GGYRCC 1 cut(s) 961
BspTNI GGTCTC 1 cut(s) 259
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 1 cut(s) 899
BssECI CCNNGG 4 cut(s) 82, 266, 965, 1155
BssMI GATC 2 cut(s) 418, 1123
BssT1I CCWWGG 2 cut(s) 965, 1155
Bst2UI CCWGG 3 cut(s) 84, 544, 1105
Bst6I CTCTTC 1 cut(s) 751
BstAUI TGTACA 1 cut(s) 600
BstBAI YACGTR 3 cut(s) 101, 443, 671
BstBI TTCGAA 1 cut(s) 496
BstC8I GCNNGC 5 cut(s) 525, 653, 756, 760, 879
BstDEI CTNAG 5 cut(s) 119, 352, 507, 974, 1011
BstF5I GGATG 3 cut(s) 235, 572, 691
BstKTI GATC 2 cut(s) 421, 1126
BstMAI GTCTC 2 cut(s) 259, 303
BstMBI GATC 2 cut(s) 418, 1123
BstMWI GCNNNNNNNGC 4 cut(s) 104, 467, 768, 1064
BstNI CCWGG 3 cut(s) 84, 544, 1105
BstNSI RCATGY 3 cut(s) 362, 601, 758
BstSCI CCNGG 5 cut(s) 82, 501, 542, 888, 1103
BstSFI CTRYAG 1 cut(s) 1059
BstV1I GCAGC 3 cut(s) 854, 982, 1045
BstX2I RGATCY 1 cut(s) 1123
BstYI RGATCY 1 cut(s) 1123
BsuRI GGCC 2 cut(s) 124, 527
BtsCI GGATG 3 cut(s) 235, 572, 691
Cac8I GCNNGC 5 cut(s) 525, 653, 756, 760, 879
Cfr13I GGNCC 3 cut(s) 51, 123, 886
CsiI ACCWGGT 1 cut(s) 1103
Csp6I GTAC 3 cut(s) 87, 601, 962
CspCI CAANNNNNGTGG 2 cut(s) 819, 854
CviAII CATG 6 cut(s) 359, 376, 598, 648, 755, 1078
CviQI GTAC 3 cut(s) 87, 601, 962
DdeI CTNAG 5 cut(s) 119, 352, 507, 974, 1011
DpnI GATC 2 cut(s) 420, 1125
DpnII GATC 2 cut(s) 418, 1123
Eam1104I CTCTTC 1 cut(s) 751
EarI CTCTTC 1 cut(s) 751
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 2 cut(s) 965, 1155
Eco24I GRGCYC 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 51, 886
Eco53kI GAGCTC 1 cut(s) 370
Eco72I CACGTG 1 cut(s) 101
Eco88I CYCGRG 1 cut(s) 882
EcoICRI GAGCTC 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 123
EcoRII CCWGG 3 cut(s) 82, 542, 1103
EcoT14I CCWWGG 2 cut(s) 965, 1155
EcoT38I GRGCYC 1 cut(s) 372
ErhI CCWWGG 2 cut(s) 965, 1155
FaeI CATG 6 cut(s) 362, 379, 601, 651, 758, 1081
FaqI GGGAC 3 cut(s) 629, 905, 1124
FatI CATG 6 cut(s) 358, 375, 597, 647, 754, 1077
FauI CCCGC 1 cut(s) 751
Fnu4HI GCNGC 3 cut(s) 843, 971, 1059
FokI GGATG 3 cut(s) 242, 559, 678
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 3 cut(s) 843, 971, 1059
FspBI CTAG 3 cut(s) 194, 260, 878
GluI GCNGC 3 cut(s) 843, 971, 1059
GsuI CTGGAG 2 cut(s) 323, 565
HaeIII GGCC 2 cut(s) 124, 527
HapII CCGG 2 cut(s) 502, 889
Hin1II CATG 6 cut(s) 362, 379, 601, 651, 758, 1081
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 5 cut(s) 4, 296, 664, 920, 1151
HpaII CCGG 2 cut(s) 502, 889
HphI GGTGA 1 cut(s) 448
Hpy166II GTNNAC 3 cut(s) 51, 919, 1031
Hpy188I TCNGA 4 cut(s) 229, 788, 1014, 1146
Hpy188III TCNNGA 3 cut(s) 302, 614, 1043
Hpy8I GTNNAC 3 cut(s) 51, 919, 1031
HpyAV CCTTC 4 cut(s) 64, 196, 248, 953
HpyCH4IV ACGT 3 cut(s) 100, 442, 670
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 467, 768, 1064
HpyF3I CTNAG 5 cut(s) 119, 352, 507, 974, 1011
HpySE526I ACGT 3 cut(s) 100, 442, 670
Hsp92II CATG 6 cut(s) 362, 379, 601, 651, 758, 1081
KpnI GGTACC 1 cut(s) 965
Kzo9I GATC 2 cut(s) 418, 1123
LguI GCTCTTC 1 cut(s) 751
LmnI GCTCC 1 cut(s) 304
Lsp1109I GCAGC 3 cut(s) 854, 982, 1045
LweI GCATC 6 cut(s) 341, 700, 780, 1005, 1009, 1054
MabI ACCWGGT 1 cut(s) 1103
MaeI CTAG 3 cut(s) 194, 260, 878
MaeII ACGT 3 cut(s) 100, 442, 670
MaeIII GTNAC 1 cut(s) 281
MalI GATC 2 cut(s) 420, 1125
MboI GATC 2 cut(s) 418, 1123
MboII GAAGA 3 cut(s) 738, 812, 1080
MflI RGATCY 1 cut(s) 1123
MhlI GDGCHC 1 cut(s) 372
MlyI GAGTC 4 cut(s) 305, 658, 914, 1145
MmeI TCCRAC 1 cut(s) 1124
MroXI GAANNNNTTC 2 cut(s) 586, 635
MseI TTAA 7 cut(s) 155, 209, 551, 555, 684, 720, 1100
MslI CAYNNNNRTG 3 cut(s) 268, 290, 340
MspA1I CMGCKG 1 cut(s) 845
MspI CCGG 2 cut(s) 502, 889
MspR9I CCNGG 5 cut(s) 84, 503, 544, 890, 1105
MvaI CCWGG 3 cut(s) 84, 544, 1105
MwoI GCNNNNNNNGC 4 cut(s) 104, 467, 768, 1064
NciI CCSGG 2 cut(s) 503, 890
NdeII GATC 2 cut(s) 418, 1123
NheI GCTAGC 1 cut(s) 877
NlaIII CATG 6 cut(s) 362, 379, 601, 651, 758, 1081
NlaIV GGNNCC 4 cut(s) 125, 388, 887, 963
NmeAIII GCCGAG 2 cut(s) 351, 902
NmuCI GTSAC 1 cut(s) 281
NspI RCATGY 3 cut(s) 362, 601, 758
NspV TTCGAA 1 cut(s) 496
OliI CACNNNNGTG 1 cut(s) 268
PacI TTAATTAA 1 cut(s) 555
PaeI GCATGC 1 cut(s) 758
PciSI GCTCTTC 1 cut(s) 751
PdmI GAANNNNTTC 2 cut(s) 586, 635
PfeI GAWTC 1 cut(s) 4
PflMI CCANNNNNTGG 1 cut(s) 704
PfoI TCCNGGA 1 cut(s) 888
PkrI GCNGC 3 cut(s) 844, 972, 1060
PleI GAGTC 4 cut(s) 304, 658, 914, 1145
PmaCI CACGTG 1 cut(s) 101
PmlI CACGTG 1 cut(s) 101
PpsI GAGTC 4 cut(s) 304, 658, 914, 1145
Ppu21I YACGTR 3 cut(s) 101, 443, 671
PshBI ATTAAT 3 cut(s) 155, 551, 720
Psp124BI GAGCTC 1 cut(s) 372
Psp6I CCWGG 3 cut(s) 82, 542, 1103
PspCI CACGTG 1 cut(s) 101
PspGI CCWGG 3 cut(s) 82, 542, 1103
PspN4I GGNNCC 4 cut(s) 125, 388, 887, 963
PspPI GGNCC 3 cut(s) 51, 123, 886
PstI CTGCAG 1 cut(s) 1063
PsuI RGATCY 1 cut(s) 1123
RsaI GTAC 3 cut(s) 88, 602, 963
RsaNI GTAC 3 cut(s) 87, 601, 962
RseI CAYNNNNRTG 3 cut(s) 268, 290, 340
SacI GAGCTC 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 751
SaqAI TTAA 7 cut(s) 155, 209, 551, 555, 684, 720, 1100
SatI GCNGC 3 cut(s) 843, 971, 1059
Sau3AI GATC 2 cut(s) 418, 1123
Sau96I GGNCC 3 cut(s) 51, 123, 886
SchI GAGTC 4 cut(s) 305, 658, 914, 1145
ScrFI CCNGG 5 cut(s) 84, 503, 544, 890, 1105
SduI GDGCHC 1 cut(s) 372
SexAI ACCWGGT 1 cut(s) 1103
SfaNI GCATC 6 cut(s) 341, 700, 780, 1005, 1009, 1054
SfcI CTRYAG 1 cut(s) 1059
SfuI TTCGAA 1 cut(s) 496
SinI GGWCC 2 cut(s) 51, 886
SmiMI CAYNNNNRTG 3 cut(s) 268, 290, 340
SpeI ACTAGT 1 cut(s) 193
SphI GCATGC 1 cut(s) 758
SsiI CCGC 4 cut(s) 96, 459, 758, 845
SspMI CTAG 3 cut(s) 194, 260, 878
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 5 cut(s) 82, 501, 542, 888, 1103
StyI CCWWGG 2 cut(s) 965, 1155
TaiI ACGT 3 cut(s) 103, 445, 673
TaqI TCGA 3 cut(s) 496, 613, 678
TatI WGTACW 1 cut(s) 600
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 7 cut(s) 155, 209, 551, 555, 684, 720, 1100
Tru9I TTAA 7 cut(s) 155, 209, 551, 555, 684, 720, 1100
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 3 cut(s) 842, 970, 1058
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 3 cut(s) 17, 927, 935
Van91I CCANNNNNTGG 1 cut(s) 704
VpaK11BI GGWCC 2 cut(s) 51, 886
VspI ATTAAT 3 cut(s) 155, 551, 720
XapI RAATTY 1 cut(s) 899
XceI RCATGY 3 cut(s) 362, 601, 758
XcmI CCANNNNNNNNNTGG 1 cut(s) 705
XmnI GAANNNNTTC 2 cut(s) 586, 635
XspI CTAG 3 cut(s) 194, 260, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.