Rorug05G0583600

nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0015047
Physical Location & Seq
Forward (+)
78067178 .. 78069967
2790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0583600.1

Sequence Viewer

Length: 1059 bp
ATGGCGGAGCACATACCTGAAGATGTGTTGAAAAAAATCTTCCAGAGGTTGCCTATCAAATCCTTAATCCGCTTCAGCTCCCTTTCGAAGCGCTGGCGCTTCATCATACTCCGCGACCGTCAGTTCGCCACATCCCAGTACCAAATCGCTTCCGGCAACAACAAAAGTGTCTGTCTCCACATCGGCTTTAAGTCTGACTCCTTCCAATCGCTGAACTTGCAAACAACGACGTCGGCTTTGAGAAATCTTCGCTACCCATTCAGGCAACCTCACGTTTACTTACTCTCATCATGCCGTGGTTTGGTGTTTGCAAGTTTCAAAGCGTGGGGATTACAAGCTTATATGTGGAACCCATCAACTGGGTTCTTCAAAAAACTTCCTCATCCAGATGCTGACGTCATGTCCTCAAACATAACTGGTTTTGGGTATATCTCGGCCACCGACGACTACAAGATTCTCATGGGTTCTTCAAAAAAATTCTGTATCTCGGCCAGGTACGGGGAGGTACAGAAGGCCAAGATCTGGATCTTCTCGTCGAAATCCAACTCTTGGAAGAGGATTCAAGACTGTCAATCGCCCGTCTGTCCAAAGACAACCGAGGGGAGTCTTTTAAACGAGGCATTTCATTGGCTGGAGACGTCTTGTGTCGTTGCTTTTGATTTAGCAGATGACAAGTTCCGGAAAGTGCCGCTGCCTGTTGACTTTGCTGCTGACTATTTCAGACGTCTAGGGGACTTTGGAGGTTGTCTGTGTGTGTTTGGTCTGGCTGATACTACTGCTGGCTCTTTGGATTTGTGGGTCATGAGGGAATATGATGTGGGTAACTCATGGACTAAGCTGTTTAACATAAAGTATACCAATCAGCCTGAGCGGATATATTATGTCAACCCAATCTTTGTTATGGAAACTAGTACATTCCTGGTGATAAATTCCGACTCTCTAACGGGGGTGAAGTTGGTAAGGAGTTATCATAACGAAGACAAGATCGAAGACATTCATTATAATGAGGATATATTCTCAATGATTGGATATGAAGAGAGCCTACTTTGGCTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

352

Amino Acids

40.53

Weight (kDa)

8.64

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 3 - 39 1.4e-06 F-box-like
F-box PF00646 4 - 39 1.2e-10 F-box domain
FBA_1 PF07734 67 - 336 2.4e-19 F-box associated beta propeller domain
FBA_3 PF08268 74 - 281 1.6e-19 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19120
fragaria_vesca FvH4_2g06711
prunus_persica Prupe.8G119100_v2.0.a1
pyrus_communis pycom05g06910
rosa_chinensis RchiOBHm_Chr6g0258821
rosa_laevigata RLG00000014583
rosa_multiflora Rmu_co8359799.1_g000001 Rmu_sc0004485.1_g000014
rosa_roxburghii Rroxscaffold_7G00206090
rosa_rugosa Rorug05G0583500 Rorug05G0583600
rosa_samantha Rh6AG100100 Rh6BG091600 Rh6CG089100 Rh6DG083400
rosa_wichuraiana Rw6G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1002
AatII GACGTC 4 cut(s) 233, 399, 641, 727
AccB7I CCANNNNNTGG 3 cut(s) 359, 522, 549
AccBSI CCGCTC 1 cut(s) 871
AccI GTMKAC 1 cut(s) 854
AccII CGCG 1 cut(s) 114
AccIII TCCGGA 1 cut(s) 678
AciI CCGC 5 cut(s) 5, 70, 112, 689, 871
AclWI GGATC 1 cut(s) 533
AcoI YGGCCR 2 cut(s) 435, 489
AcsI RAATTY 2 cut(s) 476, 928
AcuI CTGAAG 2 cut(s) 39, 58
AcyI GRCGYC 4 cut(s) 230, 396, 638, 724
AfaI GTAC 4 cut(s) 140, 497, 507, 913
AfeI AGCGCT 1 cut(s) 92
AfiI CCNNNNNNNGG 5 cut(s) 301, 359, 498, 522, 549
AgsI TTSAA 5 cut(s) 31, 319, 370, 471, 563
AhdI GACNNNNNGTC 1 cut(s) 400
AhlI ACTAGT 1 cut(s) 908
AjnI CCWGG 2 cut(s) 491, 918
AluBI AGCT 3 cut(s) 78, 338, 838
AluI AGCT 3 cut(s) 78, 338, 838
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 2 cut(s) 179, 629
AlwI GGATC 1 cut(s) 533
AlwNI CAGNNNCTG 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 678
Aor51HI AGCGCT 1 cut(s) 92
AoxI GGCC 3 cut(s) 435, 489, 513
ApeKI GCWGC 2 cut(s) 691, 707
ApoI RAATTY 2 cut(s) 476, 928
ArsI GACNNNNNNTTYG 4 cut(s) 107, 139, 220, 252
Asp700I GAANNNNTTC 1 cut(s) 993
AspLEI GCGC 2 cut(s) 93, 99
AsuHPI GGTGA 2 cut(s) 934, 961
AsuII TTCGAA 1 cut(s) 86
BarI GAAGNNNNNNTAC 2 cut(s) 1026, 1058
BbsI GAAGAC 2 cut(s) 984, 996
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 678, 694
BccI CCATC 1 cut(s) 361
BceAI ACGGC 1 cut(s) 279
BciT130I CCWGG 2 cut(s) 493, 920
BcoDI GTCTC 2 cut(s) 179, 629
BcuI ACTAGT 1 cut(s) 908
BfaI CTAG 2 cut(s) 728, 909
BfoI RGCGCY 2 cut(s) 94, 100
BglII AGATCT 1 cut(s) 519
BisI GCNGC 3 cut(s) 689, 692, 708
BlsI GCNGC 3 cut(s) 690, 693, 709
Bme1390I CCNGG 2 cut(s) 493, 920
BmeRI GACNNNNNGTC 1 cut(s) 400
BmiI GGNNCC 1 cut(s) 350
BmrFI CCNGG 2 cut(s) 493, 920
BmrI ACTGGG 2 cut(s) 130, 369
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 2 cut(s) 130, 369
BpiI GAAGAC 2 cut(s) 984, 996
BpmI CTGGAG 1 cut(s) 653
Bpu10I CCTNAGC 1 cut(s) 867
Bpu14I TTCGAA 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 524
BsaHI GRCGYC 4 cut(s) 230, 396, 638, 724
BsaJI CCNNGG 2 cut(s) 295, 597
BsaWI WCCGGW 1 cut(s) 678
Bsc4I CCNNNNNNNGG 5 cut(s) 301, 359, 498, 522, 549
Bse1I ACTGG 3 cut(s) 136, 364, 421
Bse8I GATNNNNATC 1 cut(s) 524
BseAI TCCGGA 1 cut(s) 678
BseBI CCWGG 2 cut(s) 493, 920
BseDI CCNNGG 2 cut(s) 295, 597
BseGI GGATG 2 cut(s) 131, 382
BseJI GATNNNNATC 1 cut(s) 524
BseLI CCNNNNNNNGG 5 cut(s) 301, 359, 498, 522, 549
BseMII CTCAG 1 cut(s) 858
BseNI ACTGG 3 cut(s) 136, 364, 421
BseXI GCAGC 2 cut(s) 678, 694
Bsh1236I CGCG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 3 cut(s) 437, 491, 515
BsiEI CGRYCG 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 2 cut(s) 153, 679
BslFI GGGAC 1 cut(s) 746
BslI CCNNNNNNNGG 5 cut(s) 301, 359, 498, 522, 549
BsmAI GTCTC 2 cut(s) 179, 629
BsmBI CGTCTC 1 cut(s) 629
BsmFI GGGAC 1 cut(s) 746
BsnI GGCC 3 cut(s) 437, 491, 515
Bsp119I TTCGAA 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 12
Bsp13I TCCGGA 1 cut(s) 678
Bsp143I GATC 3 cut(s) 519, 525, 984
BspACI CCGC 5 cut(s) 5, 70, 112, 689, 871
BspANI GGCC 3 cut(s) 437, 491, 515
BspCNI CTCAG 1 cut(s) 859
BspEI TCCGGA 1 cut(s) 678
BspFNI CGCG 1 cut(s) 114
BspHI TCATGA 1 cut(s) 801
BspLI GGNNCC 1 cut(s) 350
BspPI GGATC 1 cut(s) 533
BspT104I TTCGAA 1 cut(s) 86
BsrBI CCGCTC 1 cut(s) 871
BsrI ACTGG 3 cut(s) 136, 364, 421
BssECI CCNNGG 2 cut(s) 295, 597
BssMI GATC 3 cut(s) 519, 525, 984
BssNAI GTATAC 1 cut(s) 855
BssNI GRCGYC 4 cut(s) 230, 396, 638, 724
Bst1107I GTATAC 1 cut(s) 855
Bst2UI CCWGG 2 cut(s) 493, 920
Bst4CI ACNGT 2 cut(s) 119, 569
Bst6I CTCTTC 2 cut(s) 548, 1029
BstACI GRCGYC 4 cut(s) 230, 396, 638, 724
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 2 cut(s) 95, 781
BstDEI CTNAG 2 cut(s) 834, 867
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 2 cut(s) 131, 382
BstFNI CGCG 1 cut(s) 114
BstH2I RGCGCY 2 cut(s) 94, 100
BstHHI GCGC 2 cut(s) 93, 99
BstKTI GATC 3 cut(s) 522, 528, 987
BstMAI GTCTC 2 cut(s) 179, 629
BstMBI GATC 3 cut(s) 519, 525, 984
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNI CCWGG 2 cut(s) 493, 920
BstSCI CCNGG 2 cut(s) 491, 918
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 678, 694
BstV2I GAAGAC 2 cut(s) 984, 996
BstX2I RGATCY 2 cut(s) 519, 525
BstYI RGATCY 2 cut(s) 519, 525
BstZ17I GTATAC 1 cut(s) 855
BsuRI GGCC 3 cut(s) 437, 491, 515
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 2 cut(s) 131, 382
Cac8I GCNNGC 2 cut(s) 95, 781
CaiI CAGNNNCTG 1 cut(s) 392
CciI TCATGA 1 cut(s) 801
CfoI GCGC 2 cut(s) 93, 99
Csp6I GTAC 4 cut(s) 139, 496, 506, 912
CviAII CATG 5 cut(s) 291, 400, 460, 802, 828
CviQI GTAC 4 cut(s) 139, 496, 506, 912
DdeI CTNAG 2 cut(s) 834, 867
DpnI GATC 3 cut(s) 521, 527, 986
DpnII GATC 3 cut(s) 519, 525, 984
DraI TTTAAA 1 cut(s) 612
DriI GACNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 2 cut(s) 435, 489
Eam1104I CTCTTC 2 cut(s) 548, 1029
Eam1105I GACNNNNNGTC 1 cut(s) 400
EarI CTCTTC 2 cut(s) 548, 1029
EciI GGCGGA 1 cut(s) 20
Eco47III AGCGCT 1 cut(s) 92
Eco57I CTGAAG 2 cut(s) 39, 58
EcoRII CCWGG 2 cut(s) 491, 918
Esp3I CGTCTC 1 cut(s) 629
FaeI CATG 5 cut(s) 294, 403, 463, 805, 831
FaqI GGGAC 1 cut(s) 746
FatI CATG 5 cut(s) 290, 399, 459, 801, 827
FblI GTMKAC 1 cut(s) 854
Fnu4HI GCNGC 3 cut(s) 689, 692, 708
FokI GGATG 2 cut(s) 118, 369
Fsp4HI GCNGC 3 cut(s) 689, 692, 708
FspBI CTAG 2 cut(s) 728, 909
GlaI GCGC 2 cut(s) 92, 98
GluI GCNGC 3 cut(s) 689, 692, 708
GsuI CTGGAG 1 cut(s) 653
HaeII RGCGCY 2 cut(s) 94, 100
HaeIII GGCC 3 cut(s) 437, 491, 515
HapII CCGG 2 cut(s) 153, 679
HhaI GCGC 2 cut(s) 93, 99
Hin1I GRCGYC 4 cut(s) 230, 396, 638, 724
Hin1II CATG 5 cut(s) 294, 403, 463, 805, 831
Hin6I GCGC 2 cut(s) 91, 97
HinP1I GCGC 2 cut(s) 91, 97
HincII GTYRAC 2 cut(s) 700, 886
HindII GTYRAC 2 cut(s) 700, 886
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 5 cut(s) 197, 454, 559, 604, 935
HpaII CCGG 2 cut(s) 153, 679
HphI GGTGA 2 cut(s) 934, 961
Hpy166II GTNNAC 4 cut(s) 277, 700, 855, 886
Hpy188I TCNGA 3 cut(s) 196, 722, 934
Hpy188III TCNNGA 6 cut(s) 43, 386, 523, 563, 679, 802
Hpy8I GTNNAC 4 cut(s) 277, 700, 855, 886
Hpy99I CGWCG 4 cut(s) 232, 235, 446, 538
HpyAV CCTTC 2 cut(s) 211, 505
HpyCH4III ACNGT 2 cut(s) 119, 569
HpyCH4IV ACGT 5 cut(s) 230, 273, 396, 638, 724
HpyCH4V TGCA 2 cut(s) 220, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 2 cut(s) 834, 867
HpySE526I ACGT 5 cut(s) 230, 273, 396, 638, 724
Hsp92I GRCGYC 4 cut(s) 230, 396, 638, 724
Hsp92II CATG 5 cut(s) 294, 403, 463, 805, 831
HspAI GCGC 2 cut(s) 91, 97
Kpn2I TCCGGA 1 cut(s) 678
Kzo9I GATC 3 cut(s) 519, 525, 984
LmnI GCTCC 2 cut(s) 7, 83
Lsp1109I GCAGC 2 cut(s) 678, 694
LweI GCATC 1 cut(s) 379
MaeI CTAG 2 cut(s) 728, 909
MaeII ACGT 5 cut(s) 230, 273, 396, 638, 724
MaeIII GTNAC 1 cut(s) 821
MalI GATC 3 cut(s) 521, 527, 986
MbiI CCGCTC 1 cut(s) 871
MboI GATC 3 cut(s) 519, 525, 984
MflI RGATCY 2 cut(s) 519, 525
MhlI GDGCHC 1 cut(s) 12
MluCI AATT 2 cut(s) 476, 928
MlyI GAGTC 3 cut(s) 191, 613, 929
MmeI TCCRAC 2 cut(s) 567, 957
MroI TCCGGA 1 cut(s) 678
MroXI GAANNNNTTC 1 cut(s) 993
MseI TTAA 4 cut(s) 65, 189, 611, 843
MslI CAYNNNNRTG 2 cut(s) 387, 1002
MspA1I CMGCKG 1 cut(s) 691
MspI CCGG 2 cut(s) 153, 679
MspR9I CCNGG 2 cut(s) 493, 920
MvaI CCWGG 2 cut(s) 493, 920
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 217
NdeII GATC 3 cut(s) 519, 525, 984
NlaIII CATG 5 cut(s) 294, 403, 463, 805, 831
NlaIV GGNNCC 1 cut(s) 350
NmeAIII GCCGAG 2 cut(s) 413, 467
NspV TTCGAA 1 cut(s) 86
PagI TCATGA 1 cut(s) 801
PdmI GAANNNNTTC 1 cut(s) 993
PfeI GAWTC 2 cut(s) 454, 559
PflMI CCANNNNNTGG 3 cut(s) 359, 522, 549
PkrI GCNGC 3 cut(s) 690, 693, 709
PleI GAGTC 3 cut(s) 191, 612, 929
PpsI GAGTC 3 cut(s) 191, 612, 929
PsiI TTATAA 1 cut(s) 1002
Psp6I CCWGG 2 cut(s) 491, 918
PspGI CCWGG 2 cut(s) 491, 918
PspN4I GGNNCC 1 cut(s) 350
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 2 cut(s) 519, 525
RsaI GTAC 4 cut(s) 140, 497, 507, 913
RsaNI GTAC 4 cut(s) 139, 496, 506, 912
RseI CAYNNNNRTG 2 cut(s) 387, 1002
SaqAI TTAA 4 cut(s) 65, 189, 611, 843
SatI GCNGC 3 cut(s) 689, 692, 708
Sau3AI GATC 3 cut(s) 519, 525, 984
SchI GAGTC 3 cut(s) 191, 613, 929
ScrFI CCNGG 2 cut(s) 493, 920
SduI GDGCHC 1 cut(s) 12
SfaNI GCATC 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 387, 1002
SmlI CTYRAG 1 cut(s) 1052
SmoI CTYRAG 1 cut(s) 1052
SpeI ACTAGT 1 cut(s) 908
Sse9I AATT 2 cut(s) 476, 928
SsiI CCGC 5 cut(s) 5, 70, 112, 689, 871
SspMI CTAG 2 cut(s) 728, 909
StyD4I CCNGG 2 cut(s) 491, 918
TaaI ACNGT 2 cut(s) 119, 569
TaiI ACGT 5 cut(s) 233, 276, 399, 641, 727
TaqI TCGA 3 cut(s) 86, 536, 987
TasI AATT 2 cut(s) 476, 928
TatI WGTACW 1 cut(s) 911
TauI GCSGC 1 cut(s) 691
TfiI GAWTC 2 cut(s) 454, 559
Tru1I TTAA 4 cut(s) 65, 189, 611, 843
Tru9I TTAA 4 cut(s) 65, 189, 611, 843
TseI GCWGC 2 cut(s) 691, 707
TspDTI ATGAA 4 cut(s) 91, 614, 986, 1047
Van91I CCANNNNNTGG 3 cut(s) 359, 522, 549
XapI RAATTY 2 cut(s) 476, 928
XmiI GTMKAC 1 cut(s) 854
XmnI GAANNNNTTC 1 cut(s) 993
XspI CTAG 2 cut(s) 728, 909
ZraI GACGTC 4 cut(s) 231, 397, 639, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.