Rh6BG091600

nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015047
Physical Location & Seq
Reverse (-)
14456735 .. 14458081
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG091600.1

Sequence Viewer

Length: 1347 bp
ATGGATCAATCGTTCCTCCTGATGCTCTCCAACCTCCTCCACCTCCACAACTCCATCGACCCAACCACCTCCCTCATCTCCTCCCCCACCTCCACCACCACCGCCTCCTCCGCCTCCACCTCCACCTCCCCCACCTCCCTCCTCACCTCCTCCTCCGCCGCCCCTCTCCTCTTCTTCACCATCGCCTCCGTCCTCTCCTACATCGCCTCCTCCCGCCCTTCCTCCTCCTCCTCCCCCTCCTCCCCCGCCGCCTCCTCCTCCTCCGCCGCCGCCGCCGCCGCCTCCGACTACTCCGTCTCCGCCTTCCGCGCCCTCTCCACCGAGCACATCTGGTCCCTCGAAGCCCCTCTCCGCGACGCCCAGTGGCGCTCCCTCTACGGCCTGTCGTATCCCGTCTTTACGACCGTCGTCGATAAGCTCAAACCCCACATTGCTCTCTCCAACCTCTCCCTCCCTTCCGACTACGCCGTCGCCATGGTCCTCTCCCGCCTCGCCCACGGCTTCTCCGCCAAGTCCCTCGCCGCCCGCTACTCCCTCGAGCCCTACCTCATCTCCAAAATCACCAACATGGTCACGCGCCTACTCGCCACCAAGCTCTACCCGGAGTTCATCAAGATCCCCGTCAGCCGCCGCAGACTGATCGAAACCACCCAGGCCTTCGAGGAGCTGACGTCACTCCCCAACATGTGTGGCGCCATTGATGGCAGTCCGATCAGGCTGCACAAGCAGAATCTGCCTGCGAACTACAAGTGCAGGTACGGCTACAATGCTGTTCAGCTGGAGGTTGTTGCTGACCACAAGAAGATCTTCTGGGATGTTTGCGTTAAAGCGCCGGGAGGCACCGAAGACGCCACCCATTTTCGAGAGAGTTTGTTGTATAGCAGGCTTACTTCCGGTGACATTGTGTGGGATAAGGTCATCAATGTGCGTGGTCACCCTGTGAGGCCTTACATTGTTGGGGACTGGTGCTATCCTTTGCTCAGATTCTTGTTGACACCGTTTTCGCCTAATGGGATGGGGACTCCTGCGCAGAACCTGTTCGATGGAATGCTCATGAAGGGGAGATCTGTGGTGGTTGAGGCAATTGGCCTGCTCAAAGGGAGGTGGAAGATTCTGCAGGACCTGAATGTGGGTCTTAATCATGTGCCGCAGACCATTGTTGCTTGCTGTGTGCTGCACAATTTGTGCCAGATTGCGAGGGAGCCTGAACCAGAGCTGTGGAAGGACCCTGAGGAGAATGGGATGCCTCCTAGGGTGCATGACAGTGAGAAGTCGTTGTACTACTTTGGGGAAAGCTTGAGGCAAGCATTGGCTGATGATTTGCACCAGAGGCTTTCATCGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

448

Amino Acids

49.28

Weight (kDa)

8.57

Isoelectric Point (pI)

51.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_tran PF04827 220 - 395 1.1e-07 Plant transposon protein
DDE_Tnp_4 PF13359 247 - 394 5.8e-18 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19120
fragaria_vesca FvH4_2g06711
prunus_persica Prupe.8G119100_v2.0.a1
pyrus_communis pycom05g06910
rosa_chinensis RchiOBHm_Chr6g0258821
rosa_laevigata RLG00000014583
rosa_multiflora Rmu_co8359799.1_g000001 Rmu_sc0004485.1_g000014
rosa_roxburghii Rroxscaffold_7G00206090
rosa_rugosa Rorug05G0583500 Rorug05G0583600
rosa_samantha Rh6AG100100 Rh6BG091600 Rh6CG089100 Rh6DG083400
rosa_wichuraiana Rw6G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 293, 467
AatII GACGTC 1 cut(s) 674
Acc16I TGCGCA 1 cut(s) 1029
Acc36I ACCTGC 1 cut(s) 744
AccB1I GGYRCC 2 cut(s) 692, 839
AccII CGCG 3 cut(s) 309, 354, 577
AclWI GGATC 2 cut(s) 12, 610
AcyI GRCGYC 4 cut(s) 357, 671, 693, 849
AdeI CACNNNGTG 1 cut(s) 940
AfaI GTAC 2 cut(s) 758, 1280
AfiI CCNNNNNNNGG 1 cut(s) 1129
AflIII ACRYGT 1 cut(s) 684
AjnI CCWGG 1 cut(s) 651
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 6 cut(s) 418, 595, 667, 778, 1216, 1296
AluI AGCT 6 cut(s) 418, 595, 667, 778, 1216, 1296
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 1 cut(s) 301
AlwI GGATC 2 cut(s) 12, 610
AlwNI CAGNNNCTG 3 cut(s) 733, 1036, 1123
Ama87I CYCGRG 1 cut(s) 536
AoxI GGCC 4 cut(s) 379, 654, 944, 1087
ApeKI GCWGC 2 cut(s) 718, 1174
ArsI GACNNNNNNTTYG 2 cut(s) 985, 1017
Asp700I GAANNNNTTC 2 cut(s) 806, 1037
AspA2I CCTAGG 1 cut(s) 1250
AspLEI GCGC 6 cut(s) 311, 369, 579, 695, 832, 1030
AspS9I GGNCC 4 cut(s) 333, 478, 1120, 1225
AsuC2I CCSGG 2 cut(s) 602, 834
AsuHPI GGTGA 5 cut(s) 136, 169, 553, 908, 926
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 4 cut(s) 333, 478, 1120, 1225
AvrII CCTAGG 1 cut(s) 1250
AxyI CCTNAGG 1 cut(s) 1230
BanI GGYRCC 2 cut(s) 692, 839
BanII GRGCYC 1 cut(s) 543
BarI GAAGNNNNNNTAC 2 cut(s) 1262, 1294
BbsI GAAGAC 1 cut(s) 852
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 705, 1161
BccI CCATC 5 cut(s) 62, 188, 695, 1009, 1037
BceAI ACGGC 4 cut(s) 394, 452, 514, 775
BcgI CGANNNNNNTGC 4 cut(s) 622, 656, 700, 734
BciT130I CCWGG 1 cut(s) 653
BciVI GTATCC 1 cut(s) 399
BcnI CCSGG 2 cut(s) 602, 834
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 1 cut(s) 1251
BfmI CTRYAG 1 cut(s) 1115
BfoI RGCGCY 3 cut(s) 370, 696, 833
BfuAI ACCTGC 1 cut(s) 744
BfuI GTATCC 1 cut(s) 399
BglII AGATCT 2 cut(s) 804, 1064
BlnI CCTAGG 1 cut(s) 1250
Bme1390I CCNGG 3 cut(s) 602, 653, 834
Bme18I GGWCC 4 cut(s) 333, 478, 1120, 1225
BmeT110I CYCGRG 1 cut(s) 536
BmgT120I GGNCC 4 cut(s) 333, 478, 1120, 1225
BmiI GGNNCC 5 cut(s) 335, 694, 841, 1203, 1227
BmrFI CCNGG 3 cut(s) 602, 653, 834
BmrI ACTGGG 1 cut(s) 355
BmsI GCATC 2 cut(s) 12, 1233
BmuI ACTGGG 1 cut(s) 355
BoxI GACNNNNGTC 1 cut(s) 407
BpiI GAAGAC 1 cut(s) 852
BpmI CTGGAG 1 cut(s) 800
BpuEI CTTGAG 1 cut(s) 1318
BpuMI CCSGG 2 cut(s) 602, 834
BsaHI GRCGYC 4 cut(s) 357, 671, 693, 849
BsaJI CCNNGG 4 cut(s) 474, 496, 651, 1250
BsaWI WCCGGW 1 cut(s) 893
Bsc4I CCNNNNNNNGG 1 cut(s) 1129
Bse1I ACTGG 2 cut(s) 361, 968
Bse21I CCTNAGG 1 cut(s) 1230
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 1 cut(s) 653
BseDI CCNNGG 4 cut(s) 474, 496, 651, 1250
BseGI GGATG 3 cut(s) 820, 1020, 1248
BseLI CCNNNNNNNGG 1 cut(s) 1129
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 994, 1221
BseNI ACTGG 2 cut(s) 361, 968
BseXI GCAGC 2 cut(s) 705, 1161
BsgI GTGCAG 3 cut(s) 704, 772, 1160
Bsh1236I CGCG 3 cut(s) 309, 354, 577
Bsh1285I CGRYCG 1 cut(s) 405
BshFI GGCC 4 cut(s) 381, 656, 946, 1089
BshNI GGYRCC 2 cut(s) 692, 839
BsiEI CGRYCG 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 3 cut(s) 602, 833, 894
BslFI GGGAC 4 cut(s) 319, 499, 974, 1033
BslI CCNNNNNNNGG 1 cut(s) 1129
BsmAI GTCTC 1 cut(s) 301
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 4 cut(s) 319, 499, 974, 1033
BsmI GAATGC 1 cut(s) 1053
BsnI GGCC 4 cut(s) 381, 656, 946, 1089
BsoBI CYCGRG 1 cut(s) 536
Bsp1286I GDGCHC 2 cut(s) 327, 543
Bsp143I GATC 6 cut(s) 4, 615, 639, 711, 804, 1064
Bsp19I CCATGG 1 cut(s) 474
BspANI GGCC 4 cut(s) 381, 656, 946, 1089
BspCNI CTCAG 2 cut(s) 993, 1222
BspFNI CGCG 3 cut(s) 309, 354, 577
BspHI TCATGA 1 cut(s) 1053
BspLI GGNNCC 5 cut(s) 335, 694, 841, 1203, 1227
BspMAI CTGCAG 1 cut(s) 1119
BspMI ACCTGC 1 cut(s) 744
BspPI GGATC 2 cut(s) 12, 610
BspT107I GGYRCC 2 cut(s) 692, 839
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 2 cut(s) 361, 968
BssECI CCNNGG 4 cut(s) 474, 496, 651, 1250
BssMI GATC 6 cut(s) 4, 615, 639, 711, 804, 1064
BssNI GRCGYC 4 cut(s) 357, 671, 693, 849
BssT1I CCWWGG 2 cut(s) 474, 1250
Bst2UI CCWGG 1 cut(s) 653
Bst4CI ACNGT 3 cut(s) 406, 999, 1265
Bst6I CTCTTC 1 cut(s) 176
BstACI GRCGYC 4 cut(s) 357, 671, 693, 849
BstAPI GCANNNNNTGC 1 cut(s) 733
BstC8I GCNNGC 6 cut(s) 526, 738, 884, 1091, 1165, 1305
BstDEI CTNAG 2 cut(s) 980, 1230
BstDSI CCRYGG 2 cut(s) 474, 496
BstEII GGTNACC 1 cut(s) 932
BstF5I GGATG 3 cut(s) 820, 1020, 1248
BstFNI CGCG 3 cut(s) 309, 354, 577
BstH2I RGCGCY 3 cut(s) 370, 696, 833
BstHHI GCGC 6 cut(s) 311, 369, 579, 695, 832, 1030
BstKTI GATC 6 cut(s) 7, 618, 642, 714, 807, 1067
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 6 cut(s) 4, 615, 639, 711, 804, 1064
BstMCI CGRYCG 1 cut(s) 405
BstMWI GCNNNNNNNGC 9 cut(s) 110, 272, 275, 278, 308, 724, 733, 759, 1330
BstNI CCWGG 1 cut(s) 653
BstNSI RCATGY 1 cut(s) 688
BstPAI GACNNNNGTC 1 cut(s) 407
BstPI GGTNACC 1 cut(s) 932
BstSCI CCNGG 3 cut(s) 600, 651, 832
BstSFI CTRYAG 1 cut(s) 1115
BstUI CGCG 3 cut(s) 309, 354, 577
BstV1I GCAGC 2 cut(s) 705, 1161
BstV2I GAAGAC 1 cut(s) 852
BstX2I RGATCY 3 cut(s) 615, 804, 1064
BstXI CCANNNNNNTGG 1 cut(s) 1218
BstYI RGATCY 3 cut(s) 615, 804, 1064
Bsu36I CCTNAGG 1 cut(s) 1230
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 4 cut(s) 381, 656, 946, 1089
BtgI CCRYGG 2 cut(s) 474, 496
BtgZI GCGATG 2 cut(s) 166, 187
BtsCI GGATG 3 cut(s) 820, 1020, 1248
BtsIMutI CAGTG 2 cut(s) 368, 1270
BveI ACCTGC 1 cut(s) 744
Cac8I GCNNGC 6 cut(s) 526, 738, 884, 1091, 1165, 1305
CaiI CAGNNNCTG 3 cut(s) 733, 1036, 1123
CciI TCATGA 1 cut(s) 1053
CfoI GCGC 6 cut(s) 311, 369, 579, 695, 832, 1030
Cfr13I GGNCC 4 cut(s) 333, 478, 1120, 1225
CseI GACGC 2 cut(s) 365, 857
Csp6I GTAC 2 cut(s) 757, 1279
CspCI CAANNNNNGTGG 4 cut(s) 670, 705, 910, 945
CviAII CATG 6 cut(s) 475, 568, 685, 1054, 1142, 1259
CviQI GTAC 2 cut(s) 757, 1279
DdeI CTNAG 2 cut(s) 980, 1230
DinI GGCGCC 1 cut(s) 694
DpnI GATC 6 cut(s) 6, 617, 641, 713, 806, 1066
DpnII GATC 6 cut(s) 4, 615, 639, 711, 804, 1064
DraIII CACNNNGTG 1 cut(s) 940
DrdI GACNNNNNNGTC 2 cut(s) 293, 467
DseDI GACNNNNNNGTC 2 cut(s) 293, 467
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
EciI GGCGGA 5 cut(s) 100, 145, 253, 289, 496
Eco130I CCWWGG 2 cut(s) 474, 1250
Eco147I AGGCCT 2 cut(s) 656, 946
Eco24I GRGCYC 1 cut(s) 543
Eco47I GGWCC 4 cut(s) 333, 478, 1120, 1225
Eco81I CCTNAGG 1 cut(s) 1230
Eco88I CYCGRG 1 cut(s) 536
Eco91I GGTNACC 1 cut(s) 932
EcoO109I RGGNCCY 2 cut(s) 1120, 1225
EcoO65I GGTNACC 1 cut(s) 932
EcoRII CCWGG 1 cut(s) 651
EcoT14I CCWWGG 2 cut(s) 474, 1250
EcoT38I GRGCYC 1 cut(s) 543
EgeI GGCGCC 1 cut(s) 694
EheI GGCGCC 1 cut(s) 694
ErhI CCWWGG 2 cut(s) 474, 1250
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 6 cut(s) 478, 571, 688, 1057, 1145, 1262
FaiI YATR 7 cut(s) 476, 569, 686, 879, 1055, 1143, 1260
FalI AAGNNNNNCTT 2 cut(s) 791, 823
FaqI GGGAC 4 cut(s) 319, 499, 974, 1033
FatI CATG 6 cut(s) 474, 567, 684, 1053, 1141, 1258
FauI CCCGC 4 cut(s) 221, 253, 494, 533
FokI GGATG 3 cut(s) 827, 1027, 1255
FriOI GRGCYC 1 cut(s) 543
FspBI CTAG 1 cut(s) 1251
FspI TGCGCA 1 cut(s) 1029
GlaI GCGC 6 cut(s) 310, 368, 578, 694, 831, 1029
GsuI CTGGAG 1 cut(s) 800
HaeII RGCGCY 3 cut(s) 370, 696, 833
HaeIII GGCC 4 cut(s) 381, 656, 946, 1089
HapII CCGG 3 cut(s) 602, 833, 894
HgaI GACGC 2 cut(s) 365, 857
HhaI GCGC 6 cut(s) 311, 369, 579, 695, 832, 1030
Hin1I GRCGYC 4 cut(s) 357, 671, 693, 849
Hin1II CATG 6 cut(s) 478, 571, 688, 1057, 1145, 1262
Hin6I GCGC 6 cut(s) 309, 367, 577, 693, 830, 1028
HinP1I GCGC 6 cut(s) 309, 367, 577, 693, 830, 1028
HincII GTYRAC 1 cut(s) 993
HindII GTYRAC 1 cut(s) 993
HindIII AAGCTT 1 cut(s) 1294
HinfI GANTC 4 cut(s) 730, 984, 1021, 1111
HpaII CCGG 3 cut(s) 602, 833, 894
HphI GGTGA 5 cut(s) 136, 169, 553, 908, 926
Hpy166II GTNNAC 1 cut(s) 993
Hpy188I TCNGA 4 cut(s) 286, 460, 711, 983
Hpy188III TCNNGA 5 cut(s) 19, 613, 863, 1054, 1341
Hpy8I GTNNAC 1 cut(s) 993
Hpy99I CGWCG 4 cut(s) 359, 410, 413, 473
HpyAV CCTTC 6 cut(s) 228, 313, 465, 667, 1051, 1216
HpyCH4III ACNGT 3 cut(s) 406, 999, 1265
HpyCH4IV ACGT 1 cut(s) 671
HpyCH4V TGCA 6 cut(s) 721, 753, 1117, 1177, 1258, 1324
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 272, 275, 278, 308, 724, 733, 759, 1330
HpyF3I CTNAG 2 cut(s) 980, 1230
HpySE526I ACGT 1 cut(s) 671
Hsp92I GRCGYC 4 cut(s) 357, 671, 693, 849
Hsp92II CATG 6 cut(s) 478, 571, 688, 1057, 1145, 1262
HspAI GCGC 6 cut(s) 309, 367, 577, 693, 830, 1028
KasI GGCGCC 1 cut(s) 692
Kzo9I GATC 6 cut(s) 4, 615, 639, 711, 804, 1064
LmnI GCTCC 3 cut(s) 374, 664, 1201
Lsp1109I GCAGC 2 cut(s) 705, 1161
LweI GCATC 2 cut(s) 12, 1233
MaeI CTAG 1 cut(s) 1251
MaeII ACGT 1 cut(s) 671
MaeIII GTNAC 4 cut(s) 571, 672, 896, 932
MalI GATC 6 cut(s) 6, 617, 641, 713, 806, 1066
MboI GATC 6 cut(s) 4, 615, 639, 711, 804, 1064
MboII GAAGA 6 cut(s) 163, 166, 799, 814, 857, 1120
MfeI CAATTG 1 cut(s) 1083
MflI RGATCY 3 cut(s) 615, 804, 1064
MhlI GDGCHC 2 cut(s) 327, 543
MluCI AATT 2 cut(s) 1083, 1180
Mly113I GGCGCC 1 cut(s) 693
MlyI GAGTC 1 cut(s) 1015
MmeI TCCRAC 4 cut(s) 54, 309, 465, 483
MroXI GAANNNNTTC 2 cut(s) 806, 1037
MseI TTAA 2 cut(s) 825, 1137
MslI CAYNNNNRTG 3 cut(s) 566, 923, 1263
MspA1I CMGCKG 1 cut(s) 778
MspI CCGG 3 cut(s) 602, 833, 894
MspR9I CCNGG 3 cut(s) 602, 653, 834
MunI CAATTG 1 cut(s) 1083
Mva1269I GAATGC 1 cut(s) 1053
MvaI CCWGG 1 cut(s) 653
MvnI CGCG 3 cut(s) 309, 354, 577
MwoI GCNNNNNNNGC 9 cut(s) 110, 272, 275, 278, 308, 724, 733, 759, 1330
NarI GGCGCC 1 cut(s) 693
NciI CCSGG 2 cut(s) 602, 834
NcoI CCATGG 1 cut(s) 474
NdeII GATC 6 cut(s) 4, 615, 639, 711, 804, 1064
NlaIII CATG 6 cut(s) 478, 571, 688, 1057, 1145, 1262
NlaIV GGNNCC 5 cut(s) 335, 694, 841, 1203, 1227
NmuCI GTSAC 4 cut(s) 571, 672, 896, 932
NsbI TGCGCA 1 cut(s) 1029
NspI RCATGY 1 cut(s) 688
PaeR7I CTCGAG 1 cut(s) 536
PagI TCATGA 1 cut(s) 1053
PceI AGGCCT 2 cut(s) 656, 946
PciI ACATGT 1 cut(s) 684
PctI GAATGC 1 cut(s) 1053
PdmI GAANNNNTTC 2 cut(s) 806, 1037
PfeI GAWTC 3 cut(s) 730, 984, 1111
PleI GAGTC 1 cut(s) 1015
PluTI GGCGCC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 1015
PpuMI RGGWCCY 2 cut(s) 1120, 1225
PscI ACATGT 1 cut(s) 684
PshAI GACNNNNGTC 1 cut(s) 407
Psp5II RGGWCCY 2 cut(s) 1120, 1225
Psp6I CCWGG 1 cut(s) 651
PspEI GGTNACC 1 cut(s) 932
PspGI CCWGG 1 cut(s) 651
PspN4I GGNNCC 5 cut(s) 335, 694, 841, 1203, 1227
PspPI GGNCC 4 cut(s) 333, 478, 1120, 1225
PspPPI RGGWCCY 2 cut(s) 1120, 1225
PspXI VCTCGAGB 1 cut(s) 536
PstI CTGCAG 1 cut(s) 1119
PstNI CAGNNNCTG 3 cut(s) 733, 1036, 1123
PsuI RGATCY 3 cut(s) 615, 804, 1064
PvuII CAGCTG 1 cut(s) 778
RsaI GTAC 2 cut(s) 758, 1280
RsaNI GTAC 2 cut(s) 757, 1279
RseI CAYNNNNRTG 3 cut(s) 566, 923, 1263
SaqAI TTAA 2 cut(s) 825, 1137
Sau3AI GATC 6 cut(s) 4, 615, 639, 711, 804, 1064
Sau96I GGNCC 4 cut(s) 333, 478, 1120, 1225
SchI GAGTC 1 cut(s) 1015
ScrFI CCNGG 3 cut(s) 602, 653, 834
SduI GDGCHC 2 cut(s) 327, 543
SfaNI GCATC 2 cut(s) 12, 1233
SfcI CTRYAG 1 cut(s) 1115
SfoI GGCGCC 1 cut(s) 694
Sfr274I CTCGAG 1 cut(s) 536
SinI GGWCC 4 cut(s) 333, 478, 1120, 1225
SlaI CTCGAG 1 cut(s) 536
SmiMI CAYNNNNRTG 3 cut(s) 566, 923, 1263
SmlI CTYRAG 2 cut(s) 536, 1297
SmoI CTYRAG 2 cut(s) 536, 1297
Sse9I AATT 2 cut(s) 1083, 1180
SseBI AGGCCT 2 cut(s) 656, 946
SspDI GGCGCC 1 cut(s) 692
SspMI CTAG 1 cut(s) 1251
StuI AGGCCT 2 cut(s) 656, 946
StyD4I CCNGG 3 cut(s) 600, 651, 832
StyI CCWWGG 2 cut(s) 474, 1250
TaaI ACNGT 3 cut(s) 406, 999, 1265
TaiI ACGT 1 cut(s) 674
TaqI TCGA 9 cut(s) 57, 339, 411, 537, 642, 660, 862, 1041, 1340
TasI AATT 2 cut(s) 1083, 1180
TatI WGTACW 1 cut(s) 1278
TfiI GAWTC 3 cut(s) 730, 984, 1111
Tru1I TTAA 2 cut(s) 825, 1137
Tru9I TTAA 2 cut(s) 825, 1137
TscAI CASTG 2 cut(s) 368, 1270
TseFI GTSAC 4 cut(s) 571, 672, 896, 932
TseI GCWGC 2 cut(s) 718, 1174
Tsp45I GTSAC 4 cut(s) 571, 672, 896, 932
TspDTI ATGAA 3 cut(s) 598, 1070, 1326
TspGWI ACGGA 2 cut(s) 178, 283
TspRI CASTG 2 cut(s) 368, 1270
VpaK11BI GGWCC 4 cut(s) 333, 478, 1120, 1225
XceI RCATGY 1 cut(s) 688
XhoI CTCGAG 1 cut(s) 536
XmaJI CCTAGG 1 cut(s) 1250
XmnI GAANNNNTTC 2 cut(s) 806, 1037
XspI CTAG 1 cut(s) 1251
ZraI GACGTC 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.