FvH4_2g07981

metalloendopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007485
Physical Location & Seq
Forward (+)
6886117 .. 6890454
4338 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g07981.t1

Sequence Viewer

Length: 969 bp
ATGAGGTCTAGTGAGCAAATATTTATATTGAAAAGTAAAAGGGAGGACGTTGTCGTCGAACGGGAGTATGATGGAAGGCCAGTTGGTGGTCCTTGCCATGAAGGCTTGTGGTGTTGGTGGTGGAATGTGAGCGGCAATCTACCCTTGAGAACGGACGAAGAAGGTGTTGTATGTTGGTGTCACAACGTGAGTGTTTCGGCCGGGGGTCGACCGCCGATCATGCGTGGTTGTTCTTGTCTTGTCGGCGGTGGTAGTCTGGATAGATCAAGGAGGGTGGTGATTCTCCGTGTGATTGATAGGGGTGGGAGACGCAATCGTTTGGTGATTGACATTGGTGACGCGGCGGCGGACCTGCGCCACCTGCACCTTGTTGGGCCGGATGCAGTAGCTTTGACCAACCACCTTACTGCTGGAAACAACAAGAGAATGGGTGTCGTCATCCACTATCGGGCCACGACGTCGTTCTCAACTGGAAAGTCGATCGTCCCTAAAGCCATAACACTTGAAGGCAATGACCATTTCATGTGGGACGACTGGCGCGTGGCTCTTGCGCTGCAGACTCTTCACGTGCAGCTGCTGCCTTCTGGGTCCCGCGTCACCCACAAATGGGTTTCCTCCCTGCGACTCGACCCATTTTGGGTCGTGGGTCTGGTAGGAACCCATAATTGGGTATGGGTTTTTATTGTTGGAGATGAGTTTTTATGGGTTATGAGTATAGGAAAGCTTGATCTAGAAACCATCAACCTAATAACCACTCCTAGATGTGGATTTCCGGATTACAAACTCGTCAATGGATCACAGTCACGCCGTGTTCAGATTGGGCAAGCACTCAACTATGCATTCCAGCTGTGGGCAAAGGTTTCCAAGTTTACGTTTCAACAGGTTGTACCAAGTTCCCCTGCTGATCTTTTGATCAGCTTTCAGAATGGAGATCATGGTGATACTCCTGATAAAGAGCTGTGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

323

Amino Acids

36.08

Weight (kDa)

8.7

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 260 - 315 1.8e-06 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 369
AasI GACNNNNNNGTC 1 cut(s) 53
AatII GACGTC 1 cut(s) 461
Acc36I ACCTGC 2 cut(s) 360, 369
AccB7I CCANNNNNTGG 1 cut(s) 86
AccBSI CCGCTC 1 cut(s) 132
AccI GTMKAC 1 cut(s) 208
AccII CGCG 3 cut(s) 341, 540, 594
AccIII TCCGGA 1 cut(s) 772
AciI CCGC 7 cut(s) 132, 212, 246, 341, 344, 347, 592
AclWI GGATC 1 cut(s) 802
AcoI YGGCCR 1 cut(s) 198
AcvI CACGTG 1 cut(s) 568
AcyI GRCGYC 1 cut(s) 458
AdeI CACNNNGTG 2 cut(s) 187, 809
AfaI GTAC 1 cut(s) 888
AfiI CCNNNNNNNGG 9 cut(s) 86, 448, 606, 607, 637, 666, 667, 764, 850
AgsI TTSAA 3 cut(s) 31, 506, 878
AjuI GAANNNNNNNTTGG 2 cut(s) 649, 681
AluBI AGCT 6 cut(s) 389, 574, 724, 847, 918, 958
AluI AGCT 6 cut(s) 389, 574, 724, 847, 918, 958
Alw26I GTCTC 1 cut(s) 301
AlwI GGATC 1 cut(s) 802
AlwNI CAGNNNCTG 1 cut(s) 577
Aor13HI TCCGGA 1 cut(s) 772
AoxI GGCC 4 cut(s) 77, 198, 374, 450
ApeKI GCWGC 4 cut(s) 553, 571, 574, 577
AspLEI GCGC 3 cut(s) 357, 540, 553
AspS9I GGNCC 5 cut(s) 89, 349, 374, 450, 588
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 5 cut(s) 289, 334, 347, 589, 950
AvaII GGWCC 3 cut(s) 89, 349, 588
BbrPI CACGTG 1 cut(s) 568
BbvI GCAGC 4 cut(s) 540, 561, 564, 583
BccI CCATC 2 cut(s) 65, 746
BceAI ACGGC 1 cut(s) 792
BclI TGATCA 1 cut(s) 912
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 3 cut(s) 9, 731, 759
BfmI CTRYAG 1 cut(s) 554
BfuAI ACCTGC 2 cut(s) 360, 369
BglI GCCNNNNNGGC 1 cut(s) 102
BisI GCNGC 7 cut(s) 133, 342, 345, 554, 572, 575, 578
BlsI GCNGC 7 cut(s) 134, 343, 346, 555, 573, 576, 579
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 3 cut(s) 89, 349, 588
BmgT120I GGNCC 5 cut(s) 89, 349, 374, 450, 588
BmiI GGNNCC 3 cut(s) 589, 590, 658
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 166
BpuMI CCSGG 1 cut(s) 202
BsaAI YACGTR 1 cut(s) 568
BsaHI GRCGYC 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 201
BsaWI WCCGGW 1 cut(s) 772
Bsc4I CCNNNNNNNGG 9 cut(s) 86, 448, 606, 607, 637, 666, 667, 764, 850
Bse1I ACTGG 3 cut(s) 80, 475, 539
Bse3DI GCAATG 1 cut(s) 517
BseAI TCCGGA 1 cut(s) 772
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 2 cut(s) 385, 438
BseLI CCNNNNNNNGG 9 cut(s) 86, 448, 606, 607, 637, 666, 667, 764, 850
BseMI GCAATG 1 cut(s) 517
BseNI ACTGG 3 cut(s) 80, 475, 539
BseX3I CGGCCG 1 cut(s) 198
BseXI GCAGC 4 cut(s) 540, 561, 564, 583
BsgI GTGCAG 2 cut(s) 347, 590
Bsh1236I CGCG 3 cut(s) 341, 540, 594
Bsh1285I CGRYCG 3 cut(s) 201, 212, 483
BshFI GGCC 4 cut(s) 79, 200, 376, 452
BsiEI CGRYCG 3 cut(s) 201, 212, 483
BsiSI CCGG 3 cut(s) 201, 377, 773
BslFI GGGAC 3 cut(s) 470, 542, 574
BslI CCNNNNNNNGG 9 cut(s) 86, 448, 606, 607, 637, 666, 667, 764, 850
BsmAI GTCTC 1 cut(s) 301
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 3 cut(s) 470, 542, 574
BsmI GAATGC 1 cut(s) 839
BsnI GGCC 4 cut(s) 79, 200, 376, 452
Bsp13I TCCGGA 1 cut(s) 772
Bsp143I GATC 8 cut(s) 216, 263, 480, 727, 794, 904, 912, 931
BspACI CCGC 7 cut(s) 132, 212, 246, 341, 344, 347, 592
BspANI GGCC 4 cut(s) 79, 200, 376, 452
BspEI TCCGGA 1 cut(s) 772
BspFNI CGCG 3 cut(s) 341, 540, 594
BspLI GGNNCC 3 cut(s) 589, 590, 658
BspMAI CTGCAG 1 cut(s) 558
BspMI ACCTGC 2 cut(s) 360, 369
BspPI GGATC 1 cut(s) 802
BsrBI CCGCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 517
BsrI ACTGG 3 cut(s) 80, 475, 539
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 8 cut(s) 216, 263, 480, 727, 794, 904, 912, 931
BssNI GRCGYC 1 cut(s) 458
Bst4CI ACNGT 1 cut(s) 801
Bst6I CTCTTC 1 cut(s) 567
BstACI GRCGYC 1 cut(s) 458
BstAPI GCANNNNNTGC 1 cut(s) 577
BstBAI YACGTR 1 cut(s) 568
BstC8I GCNNGC 1 cut(s) 825
BstF5I GGATG 2 cut(s) 385, 438
BstFNI CGCG 3 cut(s) 341, 540, 594
BstHHI GCGC 3 cut(s) 357, 540, 553
BstKTI GATC 8 cut(s) 219, 266, 483, 730, 797, 907, 915, 934
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 8 cut(s) 216, 263, 480, 727, 794, 904, 912, 931
BstMCI CGRYCG 3 cut(s) 201, 212, 483
BstMWI GCNNNNNNNGC 4 cut(s) 102, 220, 361, 577
BstSCI CCNGG 1 cut(s) 200
BstSFI CTRYAG 1 cut(s) 554
BstUI CGCG 3 cut(s) 341, 540, 594
BstV1I GCAGC 4 cut(s) 540, 561, 564, 583
BstZI CGGCCG 1 cut(s) 198
BsuRI GGCC 4 cut(s) 79, 200, 376, 452
BtsCI GGATG 2 cut(s) 385, 438
BveI ACCTGC 2 cut(s) 360, 369
Cac8I GCNNGC 1 cut(s) 825
CaiI CAGNNNCTG 1 cut(s) 577
CfoI GCGC 3 cut(s) 357, 540, 553
Cfr13I GGNCC 5 cut(s) 89, 349, 374, 450, 588
CseI GACGC 3 cut(s) 318, 347, 583
Csp6I GTAC 1 cut(s) 887
CspCI CAANNNNNGTGG 2 cut(s) 255, 290
CviAII CATG 4 cut(s) 98, 220, 523, 935
CviQI GTAC 1 cut(s) 887
DpnI GATC 8 cut(s) 218, 265, 482, 729, 796, 906, 914, 933
DpnII GATC 8 cut(s) 216, 263, 480, 727, 794, 904, 912, 931
DraIII CACNNNGTG 2 cut(s) 187, 809
DrdI GACNNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 53
EaeI YGGCCR 1 cut(s) 198
EagI CGGCCG 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
EciI GGCGGA 1 cut(s) 362
EclXI CGGCCG 1 cut(s) 198
Eco47I GGWCC 3 cut(s) 89, 349, 588
Eco52I CGGCCG 1 cut(s) 198
Eco72I CACGTG 1 cut(s) 568
EcoO109I RGGNCCY 1 cut(s) 588
EcoT22I ATGCAT 1 cut(s) 841
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 4 cut(s) 101, 223, 526, 938
FaqI GGGAC 3 cut(s) 470, 542, 574
FatI CATG 4 cut(s) 97, 219, 522, 934
FauI CCCGC 1 cut(s) 599
FbaI TGATCA 1 cut(s) 912
FblI GTMKAC 1 cut(s) 208
Fnu4HI GCNGC 7 cut(s) 133, 342, 345, 554, 572, 575, 578
FokI GGATG 2 cut(s) 392, 425
Fsp4HI GCNGC 7 cut(s) 133, 342, 345, 554, 572, 575, 578
FspBI CTAG 3 cut(s) 9, 731, 759
GlaI GCGC 3 cut(s) 356, 539, 552
GluI GCNGC 7 cut(s) 133, 342, 345, 554, 572, 575, 578
HaeIII GGCC 4 cut(s) 79, 200, 376, 452
HapII CCGG 3 cut(s) 201, 377, 773
HgaI GACGC 3 cut(s) 318, 347, 583
HhaI GCGC 3 cut(s) 357, 540, 553
Hin1I GRCGYC 1 cut(s) 458
Hin1II CATG 4 cut(s) 101, 223, 526, 938
Hin6I GCGC 3 cut(s) 355, 538, 551
HinP1I GCGC 3 cut(s) 355, 538, 551
HincII GTYRAC 1 cut(s) 209
HindII GTYRAC 1 cut(s) 209
HindIII AAGCTT 1 cut(s) 722
HinfI GANTC 3 cut(s) 280, 559, 624
HpaII CCGG 3 cut(s) 201, 377, 773
HphI GGTGA 5 cut(s) 289, 334, 347, 589, 950
Hpy166II GTNNAC 2 cut(s) 209, 870
Hpy188I TCNGA 2 cut(s) 816, 924
Hpy188III TCNNGA 4 cut(s) 257, 731, 773, 947
Hpy8I GTNNAC 2 cut(s) 209, 870
Hpy99I CGWCG 3 cut(s) 59, 460, 463
HpyAV CCTTC 5 cut(s) 69, 95, 155, 500, 591
HpyCH4III ACNGT 1 cut(s) 801
HpyCH4IV ACGT 5 cut(s) 48, 186, 458, 567, 872
HpyCH4V TGCA 5 cut(s) 364, 383, 556, 571, 839
HpyF10VI GCNNNNNNNGC 4 cut(s) 102, 220, 361, 577
HpySE526I ACGT 5 cut(s) 48, 186, 458, 567, 872
Hsp92I GRCGYC 1 cut(s) 458
Hsp92II CATG 4 cut(s) 101, 223, 526, 938
HspAI GCGC 3 cut(s) 355, 538, 551
KflI GGGWCCC 1 cut(s) 588
Kpn2I TCCGGA 1 cut(s) 772
Ksp22I TGATCA 1 cut(s) 912
Kzo9I GATC 8 cut(s) 216, 263, 480, 727, 794, 904, 912, 931
Lsp1109I GCAGC 4 cut(s) 540, 561, 564, 583
LweI GCATC 1 cut(s) 370
MaeI CTAG 3 cut(s) 9, 731, 759
MaeII ACGT 5 cut(s) 48, 186, 458, 567, 872
MaeIII GTNAC 4 cut(s) 179, 335, 595, 801
MalI GATC 8 cut(s) 218, 265, 482, 729, 796, 906, 914, 933
MbiI CCGCTC 1 cut(s) 132
MboI GATC 8 cut(s) 216, 263, 480, 727, 794, 904, 912, 931
MboII GAAGA 2 cut(s) 170, 554
MluCI AATT 1 cut(s) 664
MlyI GAGTC 2 cut(s) 553, 618
MmeI TCCRAC 1 cut(s) 667
MnlI CCTC 3 cut(s) 37, 264, 625
Mph1103I ATGCAT 1 cut(s) 841
MroI TCCGGA 1 cut(s) 772
MspA1I CMGCKG 2 cut(s) 574, 847
MspI CCGG 3 cut(s) 201, 377, 773
MspR9I CCNGG 1 cut(s) 202
Mva1269I GAATGC 1 cut(s) 839
MvnI CGCG 3 cut(s) 341, 540, 594
MwoI GCNNNNNNNGC 4 cut(s) 102, 220, 361, 577
NciI CCSGG 1 cut(s) 202
NdeII GATC 8 cut(s) 216, 263, 480, 727, 794, 904, 912, 931
NlaIII CATG 4 cut(s) 101, 223, 526, 938
NlaIV GGNNCC 3 cut(s) 589, 590, 658
NmuCI GTSAC 4 cut(s) 179, 335, 595, 801
NsiI ATGCAT 1 cut(s) 841
PaqCI CACCTGC 1 cut(s) 369
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 537
PctI GAATGC 1 cut(s) 839
PfeI GAWTC 1 cut(s) 280
PflFI GACNNNGTC 1 cut(s) 50
PflMI CCANNNNNTGG 1 cut(s) 86
PkrI GCNGC 7 cut(s) 134, 343, 346, 555, 573, 576, 579
Ple19I CGATCG 1 cut(s) 483
PleI GAGTC 2 cut(s) 553, 618
PmaCI CACGTG 1 cut(s) 568
PmlI CACGTG 1 cut(s) 568
PpsI GAGTC 2 cut(s) 553, 618
Ppu21I YACGTR 1 cut(s) 568
PpuMI RGGWCCY 1 cut(s) 588
Psp5II RGGWCCY 1 cut(s) 588
PspCI CACGTG 1 cut(s) 568
PspN4I GGNNCC 3 cut(s) 589, 590, 658
PspPI GGNCC 5 cut(s) 89, 349, 374, 450, 588
PspPPI RGGWCCY 1 cut(s) 588
PstI CTGCAG 1 cut(s) 558
PstNI CAGNNNCTG 1 cut(s) 577
PsyI GACNNNGTC 1 cut(s) 50
PvuI CGATCG 1 cut(s) 483
PvuII CAGCTG 2 cut(s) 574, 847
RsaI GTAC 1 cut(s) 888
RsaNI GTAC 1 cut(s) 887
SalI GTCGAC 1 cut(s) 207
SatI GCNGC 7 cut(s) 133, 342, 345, 554, 572, 575, 578
Sau3AI GATC 8 cut(s) 216, 263, 480, 727, 794, 904, 912, 931
Sau96I GGNCC 5 cut(s) 89, 349, 374, 450, 588
SchI GAGTC 2 cut(s) 553, 618
ScrFI CCNGG 1 cut(s) 202
SfaNI GCATC 1 cut(s) 370
SfcI CTRYAG 1 cut(s) 554
SinI GGWCC 3 cut(s) 89, 349, 588
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 1 cut(s) 664
SsiI CCGC 7 cut(s) 132, 212, 246, 341, 344, 347, 592
SspI AATATT 1 cut(s) 21
SspMI CTAG 3 cut(s) 9, 731, 759
StyD4I CCNGG 1 cut(s) 200
TaaI ACNGT 1 cut(s) 801
TaiI ACGT 5 cut(s) 51, 189, 461, 570, 875
TaqI TCGA 4 cut(s) 57, 208, 479, 627
TasI AATT 1 cut(s) 664
TauI GCSGC 3 cut(s) 135, 344, 347
TfiI GAWTC 1 cut(s) 280
TseFI GTSAC 4 cut(s) 179, 335, 595, 801
TseI GCWGC 4 cut(s) 553, 571, 574, 577
Tsp45I GTSAC 4 cut(s) 179, 335, 595, 801
TspDTI ATGAA 2 cut(s) 114, 511
TspGWI ACGGA 2 cut(s) 167, 275
Tth111I GACNNNGTC 1 cut(s) 50
Van91I CCANNNNNTGG 1 cut(s) 86
VpaK11BI GGWCC 3 cut(s) 89, 349, 588
XbaI TCTAGA 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 407
XmiI GTMKAC 1 cut(s) 208
XspI CTAG 3 cut(s) 9, 731, 759
ZraI GACGTC 1 cut(s) 459
Zsp2I ATGCAT 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.