Rorug02G0513400

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007485
Physical Location & Seq
Forward (+)
64453715 .. 64454571
857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0513400.1

Sequence Viewer

Length: 450 bp
ATGAAAACACCAGCAGTTTGTCTCCTTTTCTTGCTCTCTGTTATACTGTTCAAGGGTTCCCATGCAGAGCCATACGAATACCTACAGTTTGTCTTGCAATACCCTAGAGGTTACTGTGTTAACGACAAGAAATGCATTCCACCGGCGAGATTGCCAGCCAAATTCACGGTTCATGGAATTTGGCCCACGAATATTTCCGAGCCGATTATCAGCTGTGACAAGGCACAGAAACTACACTCGTTCAACGGAAATCTGATAACTCCGTCGTTGAAAACCGATCTTCTCCAATCTTGGCCCAGCGTCCTGATAGATAAAGATGATATGTCGTTTTGGGAACACGAATATAATAAGCATGGGTCCTGTACTGCACCAGCTATTTTGAAGCAGCCGTCAAGTCCAAGATCGGTACCGACACAAAACCTCTCATCCTTTGTAAGCAGGCCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

149

Amino Acids

16.79

Weight (kDa)

8.83

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 25 - 123 2e-21 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 406
AccB1I GGYRCC 1 cut(s) 406
AcsI RAATTY 2 cut(s) 161, 177
AfaI GTAC 2 cut(s) 364, 408
AgsI TTSAA 4 cut(s) 52, 244, 271, 382
AluBI AGCT 2 cut(s) 213, 374
AluI AGCT 2 cut(s) 213, 374
Alw26I GTCTC 1 cut(s) 26
AoxI GGCC 3 cut(s) 182, 293, 440
ApeKI GCWGC 1 cut(s) 385
ApoI RAATTY 2 cut(s) 161, 177
Asp718I GGTACC 1 cut(s) 406
AspS9I GGNCC 3 cut(s) 183, 294, 357
AvaII GGWCC 1 cut(s) 357
BanI GGYRCC 1 cut(s) 406
BbvI GCAGC 1 cut(s) 397
BceAI ACGGC 1 cut(s) 373
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 83
BisI GCNGC 1 cut(s) 386
BlsI GCNGC 1 cut(s) 387
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 3 cut(s) 183, 294, 357
BmiI GGNNCC 3 cut(s) 58, 358, 408
Bse118I RCCGGY 1 cut(s) 142
BseGI GGATG 1 cut(s) 425
BseXI GCAGC 1 cut(s) 397
BseYI CCCAGC 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 351
BshFI GGCC 3 cut(s) 184, 295, 442
BshNI GGYRCC 1 cut(s) 406
BsiSI CCGG 1 cut(s) 143
BsmAI GTCTC 1 cut(s) 26
BsmI GAATGC 1 cut(s) 135
BsnI GGCC 3 cut(s) 184, 295, 442
Bsp143I GATC 2 cut(s) 277, 401
BspANI GGCC 3 cut(s) 184, 295, 442
BspLI GGNNCC 3 cut(s) 58, 358, 408
BspT107I GGYRCC 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 142
BssAI RCCGGY 1 cut(s) 142
BssMI GATC 2 cut(s) 277, 401
Bst4CI ACNGT 4 cut(s) 48, 87, 116, 169
BstC8I GCNNGC 2 cut(s) 156, 440
BstF5I GGATG 1 cut(s) 425
BstKTI GATC 2 cut(s) 280, 404
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 2 cut(s) 277, 401
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 397
BsuRI GGCC 3 cut(s) 184, 295, 442
BtsCI GGATG 1 cut(s) 425
Cac8I GCNNGC 2 cut(s) 156, 440
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 3 cut(s) 183, 294, 357
CseI GACGC 1 cut(s) 289
Csp6I GTAC 2 cut(s) 363, 407
CviAII CATG 3 cut(s) 62, 173, 353
CviJI RGCY 9 cut(s) 70, 158, 184, 202, 213, 295, 374, 388, 442
CviKI_1 RGCY 9 cut(s) 70, 158, 184, 202, 213, 295, 374, 388, 442
CviQI GTAC 2 cut(s) 363, 407
DpnI GATC 2 cut(s) 279, 403
DpnII GATC 2 cut(s) 277, 401
Eco47I GGWCC 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 357
EcoT22I ATGCAT 1 cut(s) 137
FaeI CATG 3 cut(s) 65, 176, 356
FaiI YATR 7 cut(s) 44, 63, 73, 174, 323, 345, 354
FatI CATG 3 cut(s) 61, 172, 352
Fnu4HI GCNGC 1 cut(s) 386
FokI GGATG 1 cut(s) 412
Fsp4HI GCNGC 1 cut(s) 386
FspBI CTAG 1 cut(s) 105
GluI GCNGC 1 cut(s) 386
GsaI CCCAGC 1 cut(s) 300
HaeIII GGCC 3 cut(s) 184, 295, 442
HapII CCGG 1 cut(s) 143
HgaI GACGC 1 cut(s) 289
Hin1II CATG 3 cut(s) 65, 176, 356
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HpaI GTTAAC 1 cut(s) 121
HpaII CCGG 1 cut(s) 143
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 2 cut(s) 199, 255
Hpy188III TCNNGA 1 cut(s) 304
Hpy8I GTNNAC 1 cut(s) 121
Hpy99I CGWCG 1 cut(s) 268
HpyCH4III ACNGT 4 cut(s) 48, 87, 116, 169
HpyCH4V TGCA 4 cut(s) 65, 97, 135, 368
Hsp92II CATG 3 cut(s) 65, 176, 356
KpnI GGTACC 1 cut(s) 410
KspAI GTTAAC 1 cut(s) 121
Kzo9I GATC 2 cut(s) 277, 401
LpnPI CCDG 8 cut(s) 24, 156, 168, 310, 317, 373, 384, 424
Lsp1109I GCAGC 1 cut(s) 397
MaeI CTAG 1 cut(s) 105
MaeIII GTNAC 2 cut(s) 110, 215
MalI GATC 2 cut(s) 279, 403
MboI GATC 2 cut(s) 277, 401
MboII GAAGA 1 cut(s) 272
MluCI AATT 2 cut(s) 161, 177
MnlI CCTC 2 cut(s) 101, 431
Mph1103I ATGCAT 1 cut(s) 137
MseI TTAA 1 cut(s) 120
MspA1I CMGCKG 1 cut(s) 213
MspI CCGG 1 cut(s) 143
Mva1269I GAATGC 1 cut(s) 135
NdeII GATC 2 cut(s) 277, 401
NlaIII CATG 3 cut(s) 65, 176, 356
NlaIV GGNNCC 3 cut(s) 58, 358, 408
NmuCI GTSAC 1 cut(s) 215
NsiI ATGCAT 1 cut(s) 137
PctI GAATGC 1 cut(s) 135
PkrI GCNGC 1 cut(s) 387
PpuMI RGGWCCY 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 357
PspFI CCCAGC 1 cut(s) 296
PspN4I GGNNCC 3 cut(s) 58, 358, 408
PspPI GGNCC 3 cut(s) 183, 294, 357
PspPPI RGGWCCY 1 cut(s) 357
PvuII CAGCTG 1 cut(s) 213
RsaI GTAC 2 cut(s) 364, 408
RsaNI GTAC 2 cut(s) 363, 407
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 1 cut(s) 386
Sau3AI GATC 2 cut(s) 277, 401
Sau96I GGNCC 3 cut(s) 183, 294, 357
SetI ASST 5 cut(s) 84, 112, 215, 376, 423
SfcI CTRYAG 1 cut(s) 83
SgrAI CRCCGGYG 1 cut(s) 142
SinI GGWCC 1 cut(s) 357
Sse9I AATT 2 cut(s) 161, 177
SspI AATATT 1 cut(s) 193
SspMI CTAG 1 cut(s) 105
TaaI ACNGT 4 cut(s) 48, 87, 116, 169
TasI AATT 2 cut(s) 161, 177
TatI WGTACW 1 cut(s) 362
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 1 cut(s) 385
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 2 cut(s) 17, 161
TspGWI ACGGA 2 cut(s) 252, 261
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 2 cut(s) 161, 177
XspI CTAG 1 cut(s) 105
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.