MD09G1072500.v1.1

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007485
Physical Location & Seq
Forward (+)
5047609 .. 5048511
903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1072500.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGCCACAAATATTATTTGTAATCTATTGGGAGCGTTACTTTCCTTTCTAGCAATTCATGCTTTCACAGTTCAGTCAATTCAAAAGGAAGAGGGAAAGACACTAGGGTTCGATGAGCTTAGAAGCTACCTGGGGACCTTCGGATACCTAACCGACAGTGTTCCAAAAAACTCCAGAAATTTTATCAGCAGCTTTGAGGTTGATGAGGTTTTGGAAACTGCACTGAAAAAAATTCAAAAAACATATCATCTGAATGTCACAGGAAAGCTTGATTCCGGTACGGTTGATCTAATAAGCACTCCGAGGTGTGGTGTGTCTGATAATTTTGTCAACCTAAATGCAACGGAACAACGTACACATGGTGTTCGTATGGGTTCTCTGGGTTCTCATTACACATTCTTCGAAGGAAACAGGAAATGGCCACCTACAAAGCGTAATCTTACCTACAAATTCAACGCCGGAGCCAAAAATCCCGCTGCGCCATATGCATTATCGATGGCATGTCGACAAGCATTTGATCTATGGGCCGAAGTTACCAACTTTACGTTTCAACAGGTAGTGCCGAGTTCACCAGCTGACATTTTGATTAGCTTTCAACGCCGCGATCATGGAGATGGGAGCCCATTTGACGGGGCCGGTAAGGTTTTGGCGCATGCTTTTGCTCCCACGGATGGAAGGCTGCACTTGGACGCAGATGAGAATTGGAGCTTCAGGCCTCCAAGTAAGAACCAGTACGACCTGGTGTGGGTGGCTGCGCACGAATTAGGGCATGTTCTTGGACTTCAGCACAGTACATTGTCTGATGCCATTATGTACGCCTATGTTGGTGCTGGGAAGACTAGAAGGGCGTTGCACAGTGATGATATTGCAGGGATAAAAGCTTTGTACCAACTGCAGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

301

Amino Acids

33.14

Weight (kDa)

7.18

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 140 - 296 6.2e-48 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 756
AccI GTMKAC 1 cut(s) 505
AccII CGCG 1 cut(s) 603
AciI CCGC 2 cut(s) 474, 601
AcoI YGGCCR 2 cut(s) 3, 419
AcsI RAATTY 3 cut(s) 178, 231, 449
AcuI CTGAAG 2 cut(s) 694, 767
AdeI CACNNNGTG 1 cut(s) 362
AfaI GTAC 6 cut(s) 280, 355, 734, 793, 815, 887
AfiI CCNNNNNNNGG 4 cut(s) 308, 629, 671, 745
AgsI TTSAA 5 cut(s) 83, 236, 454, 551, 596
AjnI CCWGG 2 cut(s) 129, 738
AluBI AGCT 8 cut(s) 118, 126, 192, 268, 575, 591, 708, 881
AluI AGCT 8 cut(s) 118, 126, 192, 268, 575, 591, 708, 881
AoxI GGCC 5 cut(s) 3, 419, 525, 633, 713
ApeKI GCWGC 5 cut(s) 189, 476, 679, 752, 895
ApoI RAATTY 3 cut(s) 178, 231, 449
AspLEI GCGC 3 cut(s) 481, 652, 757
AspS9I GGNCC 3 cut(s) 135, 525, 633
AsuHPI GGTGA 1 cut(s) 561
AsuII TTCGAA 1 cut(s) 402
AvaII GGWCC 1 cut(s) 135
BalI TGGCCA 2 cut(s) 5, 421
BanII GRGCYC 1 cut(s) 623
BbsI GAAGAC 1 cut(s) 842
BbvI GCAGC 4 cut(s) 201, 463, 666, 739
BccI CCATC 3 cut(s) 490, 608, 665
BceAI ACGGC 1 cut(s) 883
BciT130I CCWGG 2 cut(s) 131, 740
BciVI GTATCC 1 cut(s) 137
BfaI CTAG 3 cut(s) 50, 104, 840
BfmI CTRYAG 1 cut(s) 893
BfuI GTATCC 1 cut(s) 137
BisI GCNGC 6 cut(s) 190, 477, 601, 680, 753, 896
BlsI GCNGC 6 cut(s) 191, 478, 602, 681, 754, 897
Bme1390I CCNGG 2 cut(s) 131, 740
Bme18I GGWCC 1 cut(s) 135
BmgT120I GGNCC 3 cut(s) 135, 525, 633
BmiI GGNNCC 4 cut(s) 136, 463, 620, 634
BmrFI CCNGG 2 cut(s) 131, 740
BmsI GCATC 1 cut(s) 793
BpiI GAAGAC 1 cut(s) 842
BpmI CTGGAG 1 cut(s) 157
Bpu14I TTCGAA 1 cut(s) 402
Bsa29I ATCGAT 1 cut(s) 494
BsaJI CCNNGG 3 cut(s) 130, 302, 666
BsaWI WCCGGW 1 cut(s) 275
Bsc4I CCNNNNNNNGG 4 cut(s) 308, 629, 671, 745
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 1 cut(s) 730
BseBI CCWGG 2 cut(s) 131, 740
BseCI ATCGAT 1 cut(s) 494
BseDI CCNNGG 3 cut(s) 130, 302, 666
BseGI GGATG 1 cut(s) 676
BseLI CCNNNNNNNGG 4 cut(s) 308, 629, 671, 745
BseNI ACTGG 1 cut(s) 730
BseXI GCAGC 4 cut(s) 201, 463, 666, 739
BseYI CCCAGC 1 cut(s) 830
BsgI GTGCAG 2 cut(s) 204, 665
Bsh1236I CGCG 1 cut(s) 603
BshFI GGCC 5 cut(s) 5, 421, 527, 635, 715
BshVI ATCGAT 1 cut(s) 494
BsiSI CCGG 3 cut(s) 276, 459, 636
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 4 cut(s) 308, 629, 671, 745
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 5 cut(s) 5, 421, 527, 635, 715
Bsp119I TTCGAA 1 cut(s) 402
Bsp1286I GDGCHC 1 cut(s) 623
Bsp143I GATC 3 cut(s) 286, 517, 604
BspACI CCGC 2 cut(s) 474, 601
BspANI GGCC 5 cut(s) 5, 421, 527, 635, 715
BspDI ATCGAT 1 cut(s) 494
BspFNI CGCG 1 cut(s) 603
BspLI GGNNCC 4 cut(s) 136, 463, 620, 634
BspMAI CTGCAG 1 cut(s) 897
BspT104I TTCGAA 1 cut(s) 402
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 1 cut(s) 730
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 3 cut(s) 130, 302, 666
BssMI GATC 3 cut(s) 286, 517, 604
Bst2UI CCWGG 2 cut(s) 131, 740
Bst4CI ACNGT 5 cut(s) 70, 158, 283, 791, 857
Bst6I CTCTTC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 59
BstBI TTCGAA 1 cut(s) 402
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 1 cut(s) 119
BstDSI CCRYGG 1 cut(s) 666
BstF5I GGATG 1 cut(s) 676
BstFNI CGCG 1 cut(s) 603
BstHHI GCGC 3 cut(s) 481, 652, 757
BstKTI GATC 3 cut(s) 289, 520, 607
BstMBI GATC 3 cut(s) 286, 517, 604
BstMWI GCNNNNNNNGC 3 cut(s) 59, 485, 597
BstNI CCWGG 2 cut(s) 131, 740
BstNSI RCATGY 3 cut(s) 504, 656, 773
BstSCI CCNGG 2 cut(s) 129, 738
BstSFI CTRYAG 1 cut(s) 893
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 4 cut(s) 201, 463, 666, 739
BstV2I GAAGAC 1 cut(s) 842
Bsu15I ATCGAT 1 cut(s) 494
BsuI GTATCC 1 cut(s) 137
BsuRI GGCC 5 cut(s) 5, 421, 527, 635, 715
BsuTUI ATCGAT 1 cut(s) 494
BtgI CCRYGG 1 cut(s) 666
BtsCI GGATG 1 cut(s) 676
BtsIMutI CAGTG 3 cut(s) 163, 221, 862
Cac8I GCNNGC 1 cut(s) 654
CfoI GCGC 3 cut(s) 481, 652, 757
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 3 cut(s) 135, 525, 633
ClaI ATCGAT 1 cut(s) 494
CseI GACGC 1 cut(s) 698
CsiI ACCWGGT 1 cut(s) 738
Csp6I GTAC 6 cut(s) 279, 354, 733, 792, 814, 886
CviAII CATG 6 cut(s) 59, 359, 501, 608, 653, 770
CviQI GTAC 6 cut(s) 279, 354, 733, 792, 814, 886
DdeI CTNAG 1 cut(s) 119
DpnI GATC 3 cut(s) 288, 519, 606
DpnII GATC 3 cut(s) 286, 517, 604
DraIII CACNNNGTG 1 cut(s) 362
EaeI YGGCCR 2 cut(s) 3, 419
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco147I AGGCCT 1 cut(s) 715
Eco24I GRGCYC 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 135
Eco57I CTGAAG 2 cut(s) 694, 767
EcoO109I RGGNCCY 1 cut(s) 135
EcoRII CCWGG 2 cut(s) 129, 738
EcoT22I ATGCAT 1 cut(s) 490
EcoT38I GRGCYC 1 cut(s) 623
FaeI CATG 6 cut(s) 62, 362, 504, 611, 656, 773
FaqI GGGAC 1 cut(s) 148
FatI CATG 6 cut(s) 58, 358, 500, 607, 652, 769
FauI CCCGC 1 cut(s) 481
FauNDI CATATG 1 cut(s) 484
FblI GTMKAC 1 cut(s) 505
Fnu4HI GCNGC 6 cut(s) 190, 477, 601, 680, 753, 896
FokI GGATG 1 cut(s) 683
FriOI GRGCYC 1 cut(s) 623
Fsp4HI GCNGC 6 cut(s) 190, 477, 601, 680, 753, 896
FspBI CTAG 3 cut(s) 50, 104, 840
FspI TGCGCA 1 cut(s) 756
GlaI GCGC 3 cut(s) 480, 651, 756
GluI GCNGC 6 cut(s) 190, 477, 601, 680, 753, 896
GsaI CCCAGC 1 cut(s) 834
GsuI CTGGAG 1 cut(s) 157
HaeIII GGCC 5 cut(s) 5, 421, 527, 635, 715
HapII CCGG 3 cut(s) 276, 459, 636
HgaI GACGC 1 cut(s) 698
HhaI GCGC 3 cut(s) 481, 652, 757
Hin1II CATG 6 cut(s) 62, 362, 504, 611, 656, 773
Hin6I GCGC 3 cut(s) 479, 650, 755
HinP1I GCGC 3 cut(s) 479, 650, 755
HincII GTYRAC 2 cut(s) 331, 506
HindII GTYRAC 2 cut(s) 331, 506
HindIII AAGCTT 2 cut(s) 266, 879
HinfI GANTC 1 cut(s) 272
HpaII CCGG 3 cut(s) 276, 459, 636
HphI GGTGA 1 cut(s) 561
Hpy166II GTNNAC 4 cut(s) 331, 356, 506, 569
Hpy188I TCNGA 5 cut(s) 143, 252, 303, 319, 802
Hpy188III TCNNGA 1 cut(s) 174
Hpy8I GTNNAC 4 cut(s) 331, 356, 506, 569
HpyAV CCTTC 4 cut(s) 148, 398, 669, 837
HpyCH4III ACNGT 5 cut(s) 70, 158, 283, 791, 857
HpyCH4IV ACGT 2 cut(s) 352, 545
HpyCH4V TGCA 7 cut(s) 221, 341, 488, 682, 853, 869, 895
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 485, 597
HpyF3I CTNAG 1 cut(s) 119
HpySE526I ACGT 2 cut(s) 352, 545
Hsp92II CATG 6 cut(s) 62, 362, 504, 611, 656, 773
HspAI GCGC 3 cut(s) 479, 650, 755
Kzo9I GATC 3 cut(s) 286, 517, 604
LmnI GCTCC 5 cut(s) 32, 461, 618, 667, 705
Lsp1109I GCAGC 4 cut(s) 201, 463, 666, 739
LweI GCATC 1 cut(s) 793
MabI ACCWGGT 1 cut(s) 738
MaeI CTAG 3 cut(s) 50, 104, 840
MaeII ACGT 2 cut(s) 352, 545
MaeIII GTNAC 3 cut(s) 36, 256, 532
MalI GATC 3 cut(s) 288, 519, 606
MboI GATC 3 cut(s) 286, 517, 604
MboII GAAGA 3 cut(s) 101, 391, 847
MhlI GDGCHC 1 cut(s) 623
MlsI TGGCCA 2 cut(s) 5, 421
MluCI AATT 8 cut(s) 54, 78, 178, 231, 322, 449, 700, 761
MluNI TGGCCA 2 cut(s) 5, 421
MnlI CCTC 5 cut(s) 85, 190, 199, 297, 726
Mox20I TGGCCA 2 cut(s) 5, 421
Mph1103I ATGCAT 1 cut(s) 490
MscI TGGCCA 2 cut(s) 5, 421
MslI CAYNNNNRTG 3 cut(s) 252, 612, 858
Msp20I TGGCCA 2 cut(s) 5, 421
MspA1I CMGCKG 2 cut(s) 476, 575
MspI CCGG 3 cut(s) 276, 459, 636
MspR9I CCNGG 2 cut(s) 131, 740
MvaI CCWGG 2 cut(s) 131, 740
MvnI CGCG 1 cut(s) 603
MwoI GCNNNNNNNGC 3 cut(s) 59, 485, 597
NdeI CATATG 1 cut(s) 484
NdeII GATC 3 cut(s) 286, 517, 604
NlaIII CATG 6 cut(s) 62, 362, 504, 611, 656, 773
NlaIV GGNNCC 4 cut(s) 136, 463, 620, 634
NmeAIII GCCGAG 1 cut(s) 588
NmuCI GTSAC 1 cut(s) 256
NsbI TGCGCA 1 cut(s) 756
NsiI ATGCAT 1 cut(s) 490
NspI RCATGY 3 cut(s) 504, 656, 773
NspV TTCGAA 1 cut(s) 402
PaeI GCATGC 1 cut(s) 656
PceI AGGCCT 1 cut(s) 715
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 6 cut(s) 191, 478, 602, 681, 754, 897
PpuMI RGGWCCY 1 cut(s) 135
Psp5II RGGWCCY 1 cut(s) 135
Psp6I CCWGG 2 cut(s) 129, 738
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 2 cut(s) 129, 738
PspN4I GGNNCC 4 cut(s) 136, 463, 620, 634
PspPI GGNCC 3 cut(s) 135, 525, 633
PspPPI RGGWCCY 1 cut(s) 135
PstI CTGCAG 1 cut(s) 897
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 6 cut(s) 280, 355, 734, 793, 815, 887
RsaNI GTAC 6 cut(s) 279, 354, 733, 792, 814, 886
RseI CAYNNNNRTG 3 cut(s) 252, 612, 858
SalI GTCGAC 1 cut(s) 504
SatI GCNGC 6 cut(s) 190, 477, 601, 680, 753, 896
Sau3AI GATC 3 cut(s) 286, 517, 604
Sau96I GGNCC 3 cut(s) 135, 525, 633
ScrFI CCNGG 2 cut(s) 131, 740
SduI GDGCHC 1 cut(s) 623
SexAI ACCWGGT 1 cut(s) 738
SfaNI GCATC 1 cut(s) 793
SfcI CTRYAG 1 cut(s) 893
SfuI TTCGAA 1 cut(s) 402
SinI GGWCC 1 cut(s) 135
SmiMI CAYNNNNRTG 3 cut(s) 252, 612, 858
SphI GCATGC 1 cut(s) 656
Sse9I AATT 8 cut(s) 54, 78, 178, 231, 322, 449, 700, 761
SseBI AGGCCT 1 cut(s) 715
SsiI CCGC 2 cut(s) 474, 601
SspI AATATT 1 cut(s) 13
SspMI CTAG 3 cut(s) 50, 104, 840
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 2 cut(s) 129, 738
TaaI ACNGT 5 cut(s) 70, 158, 283, 791, 857
TaiI ACGT 2 cut(s) 355, 548
TaqI TCGA 4 cut(s) 111, 402, 494, 505
TasI AATT 8 cut(s) 54, 78, 178, 231, 322, 449, 700, 761
TatI WGTACW 1 cut(s) 791
TauI GCSGC 1 cut(s) 603
TfiI GAWTC 1 cut(s) 272
TscAI CASTG 3 cut(s) 163, 228, 862
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 5 cut(s) 189, 476, 679, 752, 895
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 1 cut(s) 47
TspGWI ACGGA 2 cut(s) 359, 683
TspRI CASTG 3 cut(s) 163, 228, 862
VpaK11BI GGWCC 1 cut(s) 135
XapI RAATTY 3 cut(s) 178, 231, 449
XceI RCATGY 3 cut(s) 504, 656, 773
XmiI GTMKAC 1 cut(s) 505
XspI CTAG 3 cut(s) 50, 104, 840
Zsp2I ATGCAT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.