Rh2BG592900

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007485
Physical Location & Seq
Reverse (-)
80981134 .. 80981433
300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG592900.1

Sequence Viewer

Length: 300 bp
ATGGCCACCTCAAAAGCATCATCTCACGTTCAAATTCACCCCTATATCACGTACAAATTCATCCCTGGAGCTCAAACTAAATACCAAATTGCTTTGTTGCAAGCATGCACGGATGCATTCAGATTATGGGGCAAGGTTTCGAAGTTTACGTTTCAAATGGTTGGACCAGAATTGCCGGCGCCAGCTGACCTGTCGATTAGCTTCCAACGCCGCAATCATGGCGATGGGTACCCGTTTGCCGGGTGGAGTTTTGGCCCACGCTTTCGCCCCGACAGACGGCAGGCTTCATTTAGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

99

Amino Acids

11.26

Weight (kDa)

10.11

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 15 - 81 6.1e-09 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 228
AccB1I GGYRCC 2 cut(s) 178, 228
AciI CCGC 1 cut(s) 211
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 33, 56
AcyI GRCGYC 1 cut(s) 179
AfaI GTAC 2 cut(s) 53, 230
AfiI CCNNNNNNNGG 2 cut(s) 239, 276
AgsI TTSAA 2 cut(s) 32, 155
AjnI CCWGG 1 cut(s) 64
AluBI AGCT 3 cut(s) 71, 185, 201
AluI AGCT 3 cut(s) 71, 185, 201
Alw21I GWGCWC 1 cut(s) 73
AoxI GGCC 2 cut(s) 3, 253
ApoI RAATTY 2 cut(s) 33, 56
Asp718I GGTACC 1 cut(s) 228
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 2 cut(s) 164, 254
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 1 cut(s) 29
AsuII TTCGAA 1 cut(s) 140
AvaII GGWCC 1 cut(s) 164
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 178, 228
BanII GRGCYC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 73
BccI CCATC 1 cut(s) 218
BceAI ACGGC 1 cut(s) 293
BciT130I CCWGG 1 cut(s) 66
BcnI CCSGG 1 cut(s) 241
BfoI RGCGCY 1 cut(s) 182
BisI GCNGC 1 cut(s) 211
BlsI GCNGC 1 cut(s) 212
Bme1390I CCNGG 2 cut(s) 66, 241
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 2 cut(s) 164, 254
BmiI GGNNCC 2 cut(s) 180, 230
BmrFI CCNGG 2 cut(s) 66, 241
BmsI GCATC 3 cut(s) 26, 103, 284
BpmI CTGGAG 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 241
BsaAI YACGTR 1 cut(s) 51
BsaHI GRCGYC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 64
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 276
Bse118I RCCGGY 1 cut(s) 175
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 2 cut(s) 60, 118
BseLI CCNNNNNNNGG 2 cut(s) 239, 276
BshFI GGCC 2 cut(s) 5, 255
BshNI GGYRCC 2 cut(s) 178, 228
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 2 cut(s) 176, 240
BslI CCNNNNNNNGG 2 cut(s) 239, 276
BsmI GAATGC 1 cut(s) 116
BsnI GGCC 2 cut(s) 5, 255
Bsp119I TTCGAA 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 73
BspACI CCGC 1 cut(s) 211
BspANI GGCC 2 cut(s) 5, 255
BspLI GGNNCC 2 cut(s) 180, 230
BspT104I TTCGAA 1 cut(s) 140
BspT107I GGYRCC 2 cut(s) 178, 228
BsrFI RCCGGY 1 cut(s) 175
BssAI RCCGGY 1 cut(s) 175
BssECI CCNNGG 1 cut(s) 64
BssNI GRCGYC 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 179
BstBAI YACGTR 1 cut(s) 51
BstBI TTCGAA 1 cut(s) 140
BstC8I GCNNGC 5 cut(s) 102, 106, 177, 183, 282
BstF5I GGATG 2 cut(s) 60, 118
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 1 cut(s) 181
BstMWI GCNNNNNNNGC 2 cut(s) 207, 219
BstNI CCWGG 1 cut(s) 66
BstNSI RCATGY 1 cut(s) 108
BstSCI CCNGG 2 cut(s) 64, 239
BsuRI GGCC 2 cut(s) 5, 255
BtgZI GCGATG 1 cut(s) 237
BtsCI GGATG 2 cut(s) 60, 118
Cac8I GCNNGC 5 cut(s) 102, 106, 177, 183, 282
CfoI GCGC 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 175
Cfr13I GGNCC 2 cut(s) 164, 254
Csp6I GTAC 2 cut(s) 52, 229
CviAII CATG 2 cut(s) 105, 218
CviJI RGCY 6 cut(s) 5, 71, 185, 201, 255, 284
CviKI_1 RGCY 6 cut(s) 5, 71, 185, 201, 255, 284
CviQI GTAC 2 cut(s) 52, 229
DinI GGCGCC 1 cut(s) 180
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 164
Eco53kI GAGCTC 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 64
EcoT22I ATGCAT 1 cut(s) 118
EcoT38I GRGCYC 1 cut(s) 73
EgeI GGCGCC 1 cut(s) 180
EheI GGCGCC 1 cut(s) 180
FaeI CATG 2 cut(s) 108, 221
FaiI YATR 4 cut(s) 45, 106, 127, 219
FatI CATG 2 cut(s) 104, 217
Fnu4HI GCNGC 1 cut(s) 211
FokI GGATG 2 cut(s) 47, 125
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 1 cut(s) 211
GlaI GCGC 1 cut(s) 180
GluI GCNGC 1 cut(s) 211
GsuI CTGGAG 1 cut(s) 87
HaeII RGCGCY 1 cut(s) 182
HaeIII GGCC 2 cut(s) 5, 255
HapII CCGG 2 cut(s) 176, 240
HhaI GCGC 1 cut(s) 181
Hin1I GRCGYC 1 cut(s) 179
Hin1II CATG 2 cut(s) 108, 221
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HpaII CCGG 2 cut(s) 176, 240
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 1 cut(s) 147
Hpy188I TCNGA 1 cut(s) 122
Hpy8I GTNNAC 1 cut(s) 147
HpyCH4IV ACGT 3 cut(s) 27, 50, 149
HpyCH4V TGCA 3 cut(s) 100, 108, 116
HpyF10VI GCNNNNNNNGC 2 cut(s) 207, 219
HpySE526I ACGT 3 cut(s) 27, 50, 149
Hsp92I GRCGYC 1 cut(s) 179
Hsp92II CATG 2 cut(s) 108, 221
HspAI GCGC 1 cut(s) 179
KasI GGCGCC 1 cut(s) 178
KpnI GGTACC 1 cut(s) 232
KroI GCCGGC 1 cut(s) 175
KroNI GCCGGC 1 cut(s) 177
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 8 cut(s) 51, 78, 180, 189, 195, 203, 253, 266
LweI GCATC 3 cut(s) 26, 103, 284
MaeII ACGT 3 cut(s) 27, 50, 149
MhlI GDGCHC 1 cut(s) 73
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 33, 56, 87, 170
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 142, 229
MnlI CCTC 1 cut(s) 19
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 118
MroNI GCCGGC 1 cut(s) 175
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 2 cut(s) 222, 292
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 185
MspI CCGG 2 cut(s) 176, 240
MspR9I CCNGG 2 cut(s) 66, 241
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 2 cut(s) 207, 219
NaeI GCCGGC 1 cut(s) 177
NarI GGCGCC 1 cut(s) 179
NciI CCSGG 1 cut(s) 241
NgoMIV GCCGGC 1 cut(s) 175
NlaIII CATG 2 cut(s) 108, 221
NlaIV GGNNCC 2 cut(s) 180, 230
NsiI ATGCAT 1 cut(s) 118
NspI RCATGY 1 cut(s) 108
NspV TTCGAA 1 cut(s) 140
PaeI GCATGC 1 cut(s) 108
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PctI GAATGC 1 cut(s) 116
PdiI GCCGGC 1 cut(s) 177
PkrI GCNGC 1 cut(s) 212
PluTI GGCGCC 1 cut(s) 182
Ppu21I YACGTR 1 cut(s) 51
Psp124BI GAGCTC 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 2 cut(s) 180, 230
PspPI GGNCC 2 cut(s) 164, 254
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 2 cut(s) 53, 230
RsaNI GTAC 2 cut(s) 52, 229
RseI CAYNNNNRTG 2 cut(s) 222, 292
SacI GAGCTC 1 cut(s) 73
SatI GCNGC 1 cut(s) 211
Sau96I GGNCC 2 cut(s) 164, 254
ScrFI CCNGG 2 cut(s) 66, 241
SduI GDGCHC 1 cut(s) 73
SetI ASST 9 cut(s) 11, 30, 53, 73, 138, 152, 187, 192, 203
SfaNI GCATC 3 cut(s) 26, 103, 284
SfoI GGCGCC 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 140
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 2 cut(s) 222, 292
SphI GCATGC 1 cut(s) 108
Sse9I AATT 4 cut(s) 33, 56, 87, 170
SsiI CCGC 1 cut(s) 211
SspDI GGCGCC 1 cut(s) 178
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 2 cut(s) 64, 239
TaiI ACGT 3 cut(s) 30, 53, 152
TaqI TCGA 2 cut(s) 140, 194
TasI AATT 4 cut(s) 33, 56, 87, 170
TauI GCSGC 1 cut(s) 213
TspDTI ATGAA 2 cut(s) 49, 276
TspGWI ACGGA 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 2 cut(s) 33, 56
XceI RCATGY 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.