RLG00000021518

metalloendopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007485
Physical Location & Seq
Reverse (-)
76143017 .. 76143697
681 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021518

Sequence Viewer

Length: 588 bp
ATGACCAAAAAAATTCCTTCTCATGTTGTGGGAGCTTTACTCATCCTTCTCTCAATTTCAAGGCAAGCTTCTGCAGTTCATGAGTCAATTCAAGTAGGAGACAGCCAGACTCTAGATCTTCATGAGCTCAGAAATTACCTGCGAGCCTTCGGATACCTAAATGCTTCGGAAGATGGAAACCTTTTGGAATCCGCACTAAGAACATTCCAAAAAACTTATCATCTAAATGCCACCGGTAAGCTGGATTCCGATACCGTTGACCTTATCACCACTCCAAGGTTCCCATTATATATAACCTTCTTTCCGGGACTTCCGAAATGGCCACCTCAAAAGCGTCATCTCACGTATAAATTTAACCATGGAGCTCAAACTAAATTCGAAATTGCTTTGATGCAAGCATGCATCGAAGCATTCGGGTTATGGACCAAAGTTTCGAAGTTTACGTTTCAAATAATAGCCAATCCGGCATGGCCAGCTGACCTTTCGATTAGCTTCCAACGCCGCAATCATGGCGATGGCACCCGTTTGATGGGCCGGGTAGAGTTTTGGCCCACTCTTTTGCCACGACGGACGGCAGGCTTCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

196

Amino Acids

22.12

Weight (kDa)

9.71

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 39 - 88 3.9e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 106 - 178 1.4e-08 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 147
AccB1I GGYRCC 1 cut(s) 518
AciI CCGC 2 cut(s) 192, 502
AcoI YGGCCR 2 cut(s) 320, 470
AcsI RAATTY 3 cut(s) 12, 350, 374
AfiI CCNNNNNNNGG 2 cut(s) 276, 529
AgeI ACCGGT 1 cut(s) 233
AgsI TTSAA 3 cut(s) 60, 92, 449
AluBI AGCT 7 cut(s) 35, 68, 127, 241, 365, 476, 492
AluI AGCT 7 cut(s) 35, 68, 127, 241, 365, 476, 492
Alw21I GWGCWC 2 cut(s) 129, 367
Alw26I GTCTC 1 cut(s) 93
AoxI GGCC 4 cut(s) 320, 470, 532, 548
ApoI RAATTY 3 cut(s) 12, 350, 374
ArsI GACNNNNNNTTYG 2 cut(s) 415, 447
AsiGI ACCGGT 1 cut(s) 233
AspS9I GGNCC 3 cut(s) 423, 532, 549
AsuC2I CCSGG 2 cut(s) 306, 536
AsuHPI GGTGA 1 cut(s) 259
AsuII TTCGAA 2 cut(s) 378, 434
AvaII GGWCC 1 cut(s) 423
BalI TGGCCA 2 cut(s) 322, 472
BanI GGYRCC 1 cut(s) 518
BanII GRGCYC 2 cut(s) 129, 367
Bbv12I GWGCWC 2 cut(s) 129, 367
BccI CCATC 3 cut(s) 167, 509, 523
BciVI GTATCC 1 cut(s) 146
BcnI CCSGG 2 cut(s) 306, 536
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 1 cut(s) 113
BfmI CTRYAG 1 cut(s) 72
BfuAI ACCTGC 1 cut(s) 147
BfuI GTATCC 1 cut(s) 146
BglI GCCNNNNNGGC 1 cut(s) 464
BglII AGATCT 1 cut(s) 115
BisI GCNGC 1 cut(s) 502
BlsI GCNGC 1 cut(s) 503
Bme1390I CCNGG 2 cut(s) 306, 536
Bme18I GGWCC 1 cut(s) 423
BmgT120I GGNCC 3 cut(s) 423, 532, 549
BmiI GGNNCC 2 cut(s) 281, 520
BmrFI CCNGG 2 cut(s) 306, 536
BmsI GCATC 2 cut(s) 381, 411
BplI GAGNNNNNCTC 2 cut(s) 24, 56
Bpu14I TTCGAA 2 cut(s) 378, 434
BpuMI CCSGG 2 cut(s) 306, 536
BsaAI YACGTR 1 cut(s) 345
BsaJI CCNNGG 2 cut(s) 275, 358
BsaWI WCCGGW 1 cut(s) 233
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 529
Bse118I RCCGGY 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 275, 358
BseGI GGATG 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 276, 529
BseMII CTCAG 1 cut(s) 142
BshFI GGCC 4 cut(s) 322, 472, 534, 550
BshNI GGYRCC 1 cut(s) 518
BshTI ACCGGT 1 cut(s) 233
BsiHKAI GWGCWC 2 cut(s) 129, 367
BsiSI CCGG 4 cut(s) 234, 305, 464, 535
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 2 cut(s) 276, 529
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 321
BsmI GAATGC 1 cut(s) 410
BsnI GGCC 4 cut(s) 322, 472, 534, 550
Bsp119I TTCGAA 2 cut(s) 378, 434
Bsp1286I GDGCHC 2 cut(s) 129, 367
Bsp143I GATC 1 cut(s) 115
Bsp19I CCATGG 1 cut(s) 358
BspACI CCGC 2 cut(s) 192, 502
BspANI GGCC 4 cut(s) 322, 472, 534, 550
BspCNI CTCAG 1 cut(s) 141
BspHI TCATGA 2 cut(s) 79, 121
BspLI GGNNCC 2 cut(s) 281, 520
BspMAI CTGCAG 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 147
BspT104I TTCGAA 2 cut(s) 378, 434
BspT107I GGYRCC 1 cut(s) 518
BsrFI RCCGGY 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 275, 358
BssMI GATC 1 cut(s) 115
BssT1I CCWWGG 2 cut(s) 275, 358
Bst4CI ACNGT 1 cut(s) 256
BstBAI YACGTR 1 cut(s) 345
BstBI TTCGAA 2 cut(s) 378, 434
BstC8I GCNNGC 6 cut(s) 66, 144, 396, 400, 474, 577
BstDEI CTNAG 3 cut(s) 128, 197, 585
BstDSI CCRYGG 1 cut(s) 358
BstF5I GGATG 1 cut(s) 42
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 4 cut(s) 464, 473, 498, 510
BstNSI RCATGY 1 cut(s) 402
BstSCI CCNGG 2 cut(s) 304, 534
BstSFI CTRYAG 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BsuI GTATCC 1 cut(s) 146
BsuRI GGCC 4 cut(s) 322, 472, 534, 550
BtgI CCRYGG 1 cut(s) 358
BtgZI GCGATG 1 cut(s) 528
BtsCI GGATG 1 cut(s) 42
BveI ACCTGC 1 cut(s) 147
Cac8I GCNNGC 6 cut(s) 66, 144, 396, 400, 474, 577
CciI TCATGA 2 cut(s) 79, 121
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 3 cut(s) 423, 532, 549
CseI GACGC 1 cut(s) 323
CspAI ACCGGT 1 cut(s) 233
CviAII CATG 7 cut(s) 23, 80, 122, 359, 399, 468, 509
DdeI CTNAG 3 cut(s) 128, 197, 585
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
EaeI YGGCCR 2 cut(s) 320, 470
Ecl136II GAGCTC 2 cut(s) 127, 365
Eco130I CCWWGG 2 cut(s) 275, 358
Eco24I GRGCYC 2 cut(s) 129, 367
Eco47I GGWCC 1 cut(s) 423
Eco53kI GAGCTC 2 cut(s) 127, 365
EcoICRI GAGCTC 2 cut(s) 127, 365
EcoT14I CCWWGG 2 cut(s) 275, 358
EcoT22I ATGCAT 1 cut(s) 404
EcoT38I GRGCYC 2 cut(s) 129, 367
ErhI CCWWGG 2 cut(s) 275, 358
FaeI CATG 7 cut(s) 26, 83, 125, 362, 402, 471, 512
FalI AAGNNNNNCTT 2 cut(s) 52, 84
FaqI GGGAC 1 cut(s) 321
FatI CATG 7 cut(s) 22, 79, 121, 358, 398, 467, 508
Fnu4HI GCNGC 1 cut(s) 502
FokI GGATG 1 cut(s) 29
FriOI GRGCYC 2 cut(s) 129, 367
Fsp4HI GCNGC 1 cut(s) 502
FspBI CTAG 1 cut(s) 113
GluI GCNGC 1 cut(s) 502
HaeIII GGCC 4 cut(s) 322, 472, 534, 550
HapII CCGG 4 cut(s) 234, 305, 464, 535
HgaI GACGC 1 cut(s) 323
Hin1II CATG 7 cut(s) 26, 83, 125, 362, 402, 471, 512
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 66
HinfI GANTC 4 cut(s) 83, 109, 188, 245
HpaII CCGG 4 cut(s) 234, 305, 464, 535
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 259, 441
Hpy188I TCNGA 5 cut(s) 131, 152, 169, 250, 315
Hpy188III TCNNGA 3 cut(s) 80, 113, 122
Hpy8I GTNNAC 2 cut(s) 259, 441
Hpy99I CGWCG 1 cut(s) 570
HpyAV CCTTC 4 cut(s) 27, 56, 157, 307
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 2 cut(s) 344, 443
HpyCH4V TGCA 3 cut(s) 74, 394, 402
HpyF10VI GCNNNNNNNGC 4 cut(s) 464, 473, 498, 510
HpyF3I CTNAG 3 cut(s) 128, 197, 585
HpySE526I ACGT 2 cut(s) 344, 443
Hsp92II CATG 7 cut(s) 26, 83, 125, 362, 402, 471, 512
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 2 cut(s) 32, 362
LpnPI CCDG 9 cut(s) 119, 152, 227, 247, 318, 477, 486, 548, 561
LweI GCATC 2 cut(s) 381, 411
MaeI CTAG 1 cut(s) 113
MaeII ACGT 2 cut(s) 344, 443
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 2 cut(s) 110, 182
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 129, 367
MlsI TGGCCA 2 cut(s) 322, 472
MluCI AATT 7 cut(s) 12, 54, 87, 133, 350, 374, 381
MluNI TGGCCA 2 cut(s) 322, 472
MlyI GAGTC 2 cut(s) 92, 103
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 1 cut(s) 336
Mox20I TGGCCA 2 cut(s) 322, 472
Mph1103I ATGCAT 1 cut(s) 404
MscI TGGCCA 2 cut(s) 322, 472
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 2 cut(s) 225, 513
Msp20I TGGCCA 2 cut(s) 322, 472
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 4 cut(s) 234, 305, 464, 535
MspR9I CCNGG 2 cut(s) 306, 536
Mva1269I GAATGC 1 cut(s) 410
MwoI GCNNNNNNNGC 4 cut(s) 464, 473, 498, 510
NciI CCSGG 2 cut(s) 306, 536
NcoI CCATGG 1 cut(s) 358
NdeII GATC 1 cut(s) 115
NlaIII CATG 7 cut(s) 26, 83, 125, 362, 402, 471, 512
NlaIV GGNNCC 2 cut(s) 281, 520
NsiI ATGCAT 1 cut(s) 404
NspI RCATGY 1 cut(s) 402
NspV TTCGAA 2 cut(s) 378, 434
PaeI GCATGC 1 cut(s) 402
PagI TCATGA 2 cut(s) 79, 121
PcsI WCGNNNNNNNCGW 1 cut(s) 440
PctI GAATGC 1 cut(s) 410
PfeI GAWTC 2 cut(s) 188, 245
PfoI TCCNGGA 1 cut(s) 304
PinAI ACCGGT 1 cut(s) 233
PkrI GCNGC 1 cut(s) 503
PleI GAGTC 2 cut(s) 91, 103
PpsI GAGTC 2 cut(s) 91, 103
Ppu21I YACGTR 1 cut(s) 345
Psp124BI GAGCTC 2 cut(s) 129, 367
PspN4I GGNNCC 2 cut(s) 281, 520
PspPI GGNCC 3 cut(s) 423, 532, 549
PstI CTGCAG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 476
RseI CAYNNNNRTG 2 cut(s) 225, 513
SacI GAGCTC 2 cut(s) 129, 367
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 1 cut(s) 502
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 3 cut(s) 423, 532, 549
SchI GAGTC 2 cut(s) 92, 103
ScrFI CCNGG 2 cut(s) 306, 536
SduI GDGCHC 2 cut(s) 129, 367
SfaNI GCATC 2 cut(s) 381, 411
SfcI CTRYAG 1 cut(s) 72
SfuI TTCGAA 2 cut(s) 378, 434
SinI GGWCC 1 cut(s) 423
SmiMI CAYNNNNRTG 2 cut(s) 225, 513
SphI GCATGC 1 cut(s) 402
Sse9I AATT 7 cut(s) 12, 54, 87, 133, 350, 374, 381
SsiI CCGC 2 cut(s) 192, 502
SspMI CTAG 1 cut(s) 113
SstI GAGCTC 2 cut(s) 129, 367
StyD4I CCNGG 2 cut(s) 304, 534
StyI CCWWGG 2 cut(s) 275, 358
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 2 cut(s) 347, 446
TaqI TCGA 4 cut(s) 378, 405, 434, 485
TasI AATT 7 cut(s) 12, 54, 87, 133, 350, 374, 381
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 2 cut(s) 188, 245
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TspDTI ATGAA 2 cut(s) 68, 110
TspGWI ACGGA 1 cut(s) 583
VpaK11BI GGWCC 1 cut(s) 423
XapI RAATTY 3 cut(s) 12, 350, 374
XbaI TCTAGA 1 cut(s) 112
XceI RCATGY 1 cut(s) 402
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XspI CTAG 1 cut(s) 113
Zsp2I ATGCAT 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.