RLG00000014425

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007485
Physical Location & Seq
Reverse (-)
53012803 .. 53013468
666 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014425

Sequence Viewer

Length: 465 bp
ATGGCAGGATGTGGTACTTGTACCCTTCGTGTTGTTCTTATGGGAGCTTTACTTCCCGTCCTAGCAATGCAGGCTTCAATAGCCGAGTTCCGTCTGTTGCATGAGGCCAAGGCGGCCATAGTGGCTGATGAGCTTAGAACCTACCTAAGAACCTTTGGATATCTTATGAAGGACCCCAAGGATAAGCAGCAGCAGCTCTTGGAATCTGCACTACAGATAGAAACCCCAATTGGGAACTACGCTTTGGCTCGAGCATGTGCTCAGGCATTCGGATTATGGGCCGGTGTTCCCAACTCTACATTCACAAGTGTCGGACTAGACGCCCCCCATCAACTGGACATTAGCTTTCGTCGGGGTCAGCATCGAGATGGGAAACCGTTTGATGGCCCAGGTGGGATATTAAGGCATGCATTCTACCCCGTGGCTGGACTTATGCATTCGGATGCAGATGAGATTGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

155

Amino Acids

16.67

Weight (kDa)

6.5

Isoelectric Point (pI)

50.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 78 - 151 3.4e-13 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 334
AciI CCGC 1 cut(s) 113
AcoI YGGCCR 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 2 cut(s) 16, 22
AfiI CCNNNNNNNGG 4 cut(s) 231, 334, 383, 425
AgsI TTSAA 1 cut(s) 78
AjnI CCWGG 1 cut(s) 388
AjuI GAANNNNNNNTTGG 6 cut(s) 213, 227, 245, 259, 284, 316
AluBI AGCT 4 cut(s) 47, 133, 196, 345
AluI AGCT 4 cut(s) 47, 133, 196, 345
Alw21I GWGCWC 1 cut(s) 262
Ama87I CYCGRG 1 cut(s) 249
AoxI GGCC 4 cut(s) 105, 114, 279, 385
ApeKI GCWGC 3 cut(s) 187, 190, 193
AspS9I GGNCC 3 cut(s) 172, 279, 386
AvaI CYCGRG 1 cut(s) 249
AvaII GGWCC 1 cut(s) 172
Bbv12I GWGCWC 1 cut(s) 262
BbvI GCAGC 3 cut(s) 199, 202, 205
BccI CCATC 3 cut(s) 336, 362, 377
BciT130I CCWGG 1 cut(s) 390
BfaI CTAG 2 cut(s) 62, 317
BfmI CTRYAG 1 cut(s) 212
BglI GCCNNNNNGGC 2 cut(s) 113, 122
BisI GCNGC 4 cut(s) 114, 188, 191, 194
BlsI GCNGC 4 cut(s) 115, 189, 192, 195
Bme1390I CCNGG 1 cut(s) 390
Bme18I GGWCC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 249
BmgT120I GGNCC 3 cut(s) 172, 279, 386
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 390
BmsI GCATC 2 cut(s) 370, 433
Bpu10I CCTNAGC 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 4 cut(s) 108, 177, 388, 420
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 334, 383, 425
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 339
Bse3DI GCAATG 1 cut(s) 72
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 4 cut(s) 108, 177, 388, 420
BseGI GGATG 2 cut(s) 14, 448
BseLI CCNNNNNNNGG 4 cut(s) 231, 334, 383, 425
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 1 cut(s) 339
BseXI GCAGC 3 cut(s) 199, 202, 205
BsgI GTGCAG 1 cut(s) 192
BshFI GGCC 4 cut(s) 107, 116, 281, 387
BsiHKAI GWGCWC 1 cut(s) 262
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 1 cut(s) 282
BslI CCNNNNNNNGG 4 cut(s) 231, 334, 383, 425
BsmI GAATGC 3 cut(s) 266, 410, 436
BsnI GGCC 4 cut(s) 107, 116, 281, 387
BsoBI CYCGRG 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 262
BspACI CCGC 1 cut(s) 113
BspANI GGCC 4 cut(s) 107, 116, 281, 387
BspCNI CTCAG 1 cut(s) 274
BspLI GGNNCC 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 72
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 1 cut(s) 339
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 4 cut(s) 108, 177, 388, 420
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 2 cut(s) 108, 177
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 321
BstC8I GCNNGC 2 cut(s) 72, 408
BstDEI CTNAG 3 cut(s) 134, 146, 261
BstDSI CCRYGG 1 cut(s) 420
BstF5I GGATG 2 cut(s) 14, 448
BstMWI GCNNNNNNNGC 5 cut(s) 71, 80, 113, 122, 193
BstNI CCWGG 1 cut(s) 390
BstNSI RCATGY 2 cut(s) 258, 410
BstSCI CCNGG 1 cut(s) 388
BstSFI CTRYAG 1 cut(s) 212
BstV1I GCAGC 3 cut(s) 199, 202, 205
BsuRI GGCC 4 cut(s) 107, 116, 281, 387
BtgI CCRYGG 1 cut(s) 420
BtsCI GGATG 2 cut(s) 14, 448
Cac8I GCNNGC 2 cut(s) 72, 408
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 3 cut(s) 172, 279, 386
CseI GACGC 1 cut(s) 329
Csp6I GTAC 2 cut(s) 15, 21
CviAII CATG 3 cut(s) 101, 255, 407
CviQI GTAC 2 cut(s) 15, 21
DdeI CTNAG 3 cut(s) 134, 146, 261
EaeI YGGCCR 1 cut(s) 114
Eco130I CCWWGG 2 cut(s) 108, 177
Eco32I GATATC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 172
Eco88I CYCGRG 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 388
EcoRV GATATC 1 cut(s) 161
EcoT14I CCWWGG 2 cut(s) 108, 177
EcoT22I ATGCAT 2 cut(s) 412, 438
ErhI CCWWGG 2 cut(s) 108, 177
FaeI CATG 3 cut(s) 104, 258, 410
FaiI YATR 8 cut(s) 41, 102, 119, 167, 256, 277, 408, 434
FatI CATG 3 cut(s) 100, 254, 406
Fnu4HI GCNGC 4 cut(s) 114, 188, 191, 194
FokI GGATG 2 cut(s) 21, 455
Fsp4HI GCNGC 4 cut(s) 114, 188, 191, 194
FspBI CTAG 2 cut(s) 62, 317
GluI GCNGC 4 cut(s) 114, 188, 191, 194
HaeIII GGCC 4 cut(s) 107, 116, 281, 387
HapII CCGG 1 cut(s) 282
HgaI GACGC 1 cut(s) 329
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 3 cut(s) 104, 258, 410
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 282
Hpy188I TCNGA 3 cut(s) 272, 314, 442
Hpy188III TCNNGA 1 cut(s) 365
Hpy99I CGWCG 1 cut(s) 354
HpyAV CCTTC 2 cut(s) 35, 163
HpyCH4III ACNGT 1 cut(s) 378
HpyCH4V TGCA 6 cut(s) 70, 100, 209, 410, 436, 446
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 80, 113, 122, 193
HpyF3I CTNAG 3 cut(s) 134, 146, 261
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 3 cut(s) 104, 258, 410
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 7 cut(s) 56, 248, 295, 320, 375, 402, 411
Lsp1109I GCAGC 3 cut(s) 199, 202, 205
LweI GCATC 2 cut(s) 370, 433
MaeI CTAG 2 cut(s) 62, 317
MfeI CAATTG 1 cut(s) 228
MhlI GDGCHC 1 cut(s) 262
MluCI AATT 1 cut(s) 228
MmeI TCCRAC 1 cut(s) 292
MnlI CCTC 1 cut(s) 97
Mph1103I ATGCAT 2 cut(s) 412, 438
MseI TTAA 1 cut(s) 401
MslI CAYNNNNRTG 2 cut(s) 366, 441
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 1 cut(s) 390
MunI CAATTG 1 cut(s) 228
Mva1269I GAATGC 3 cut(s) 266, 410, 436
MvaI CCWGG 1 cut(s) 390
MwoI GCNNNNNNNGC 5 cut(s) 71, 80, 113, 122, 193
NlaIII CATG 3 cut(s) 104, 258, 410
NlaIV GGNNCC 1 cut(s) 174
NmeAIII GCCGAG 1 cut(s) 109
NsiI ATGCAT 2 cut(s) 412, 438
NspI RCATGY 2 cut(s) 258, 410
PaeI GCATGC 1 cut(s) 410
PaeR7I CTCGAG 1 cut(s) 249
PctI GAATGC 3 cut(s) 266, 410, 436
PfeI GAWTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 4 cut(s) 115, 189, 192, 195
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 3 cut(s) 172, 279, 386
PspPPI RGGWCCY 1 cut(s) 172
PspXI VCTCGAGB 1 cut(s) 249
RsaI GTAC 2 cut(s) 16, 22
RsaNI GTAC 2 cut(s) 15, 21
RseI CAYNNNNRTG 2 cut(s) 366, 441
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 4 cut(s) 114, 188, 191, 194
Sau96I GGNCC 3 cut(s) 172, 279, 386
ScrFI CCNGG 1 cut(s) 390
SduI GDGCHC 1 cut(s) 262
SetI ASST 8 cut(s) 49, 135, 143, 147, 155, 198, 347, 394
SfaNI GCATC 2 cut(s) 370, 433
SfcI CTRYAG 1 cut(s) 212
SfiI GGCCNNNNNGGCC 1 cut(s) 113
Sfr274I CTCGAG 1 cut(s) 249
SinI GGWCC 1 cut(s) 172
SlaI CTCGAG 1 cut(s) 249
SmiMI CAYNNNNRTG 2 cut(s) 366, 441
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
SphI GCATGC 1 cut(s) 410
Sse9I AATT 1 cut(s) 228
SsiI CCGC 1 cut(s) 113
SspMI CTAG 2 cut(s) 62, 317
StyD4I CCNGG 1 cut(s) 388
StyI CCWWGG 2 cut(s) 108, 177
TaaI ACNGT 1 cut(s) 378
TaqI TCGA 2 cut(s) 250, 364
TasI AATT 1 cut(s) 228
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 1 cut(s) 203
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TseI GCWGC 3 cut(s) 187, 190, 193
TspDTI ATGAA 1 cut(s) 182
TspGWI ACGGA 1 cut(s) 80
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 172
XceI RCATGY 2 cut(s) 258, 410
XhoI CTCGAG 1 cut(s) 249
XspI CTAG 2 cut(s) 62, 317
Zsp2I ATGCAT 2 cut(s) 412, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.