Rw0G008390

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00319
OG0007485
Physical Location & Seq
Forward (+)
6829 .. 7401
573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008390.1

Sequence Viewer

Length: 573 bp
TGCGGAAAGCTGGATTCTGATACCGTTGACCTTATCACCACTCCAAGGTGCGGTGTGCCTGATAATTTTGGCCAAAATTTTATCGATTCAAATGAAACCACACATAATGGATCGTATGGACATGATGTTCATATAGGTTCCCATTATACCTTCTTTCCGGAACTTCCGAAATGGCCACAGCGTCATCTCACGTACAAATTCAACCATGCAGCTCAAACTAAATTCGAATATGCTTTGATGCAAGCATGCATGGAAGCATTCGGGTTATGGACCAAGGTCTCAAAATTTACGTTTCAAATAATAGTCAATCCGGCATGGCCAGCTGACCTTTCGATTAGCTTCCAACGCCGCAATCATGGCAATGGGCACCCGTTTGATGGGCCGGGTAGAGTTTTGGGCCACTCTTTCGCCCCTACGGACGGCAGGCTTCATTTAGATGCTGATGAAAATTGGAGCTTCAGGCCTCCGCGTGCGAGAGAGATTGGCTTAGTAGGGGTTGTTACGCGAGAACACTACGAAGTACGAACTCTCAGAGACTCAGAGAGCTCATCGAGACTAGGTGCGACGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

190

Amino Acids

21.5

Weight (kDa)

6.9

Isoelectric Point (pI)

37.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 57 - 168 5.6e-24 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 366
AccII CGCG 2 cut(s) 469, 505
AccIII TCCGGA 1 cut(s) 157
AciI CCGC 4 cut(s) 3, 51, 349, 467
AclWI GGATC 1 cut(s) 118
AcoI YGGCCR 3 cut(s) 70, 173, 317
AcsI RAATTY 4 cut(s) 76, 197, 221, 284
AcuI CTGAAG 1 cut(s) 442
AfaI GTAC 2 cut(s) 194, 522
AfiI CCNNNNNNNGG 4 cut(s) 45, 50, 377, 419
AgsI TTSAA 3 cut(s) 90, 202, 296
AloI GAACNNNNNNTCC 2 cut(s) 111, 143
AluBI AGCT 6 cut(s) 10, 212, 323, 339, 456, 546
AluI AGCT 6 cut(s) 10, 212, 323, 339, 456, 546
Alw21I GWGCWC 1 cut(s) 548
Alw26I GTCTC 3 cut(s) 283, 528, 547
AlwI GGATC 1 cut(s) 118
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 6 cut(s) 70, 173, 317, 380, 397, 461
ApeKI GCWGC 1 cut(s) 209
ApoI RAATTY 4 cut(s) 76, 197, 221, 284
AspS9I GGNCC 3 cut(s) 270, 380, 397
AsuC2I CCSGG 1 cut(s) 384
AsuHPI GGTGA 1 cut(s) 28
AsuII TTCGAA 1 cut(s) 225
AvaII GGWCC 1 cut(s) 270
BaeGI GKGCMC 1 cut(s) 369
BalI TGGCCA 3 cut(s) 72, 175, 319
BanI GGYRCC 1 cut(s) 366
BanII GRGCYC 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 548
BbvI GCAGC 1 cut(s) 221
BccI CCATC 1 cut(s) 371
BceAI ACGGC 1 cut(s) 436
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 3 cut(s) 283, 528, 547
BfaI CTAG 1 cut(s) 557
BisI GCNGC 2 cut(s) 210, 349
BlsI GCNGC 2 cut(s) 211, 350
Bme1390I CCNGG 1 cut(s) 384
Bme18I GGWCC 1 cut(s) 270
BmgT120I GGNCC 3 cut(s) 270, 380, 397
BmiI GGNNCC 2 cut(s) 139, 368
BmrFI CCNGG 1 cut(s) 384
BmsI GCATC 2 cut(s) 228, 427
BoxI GACNNNNGTC 1 cut(s) 275
Bpu14I TTCGAA 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 384
Bsa29I ATCGAT 1 cut(s) 84
BsaAI YACGTR 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 283
BsaJI CCNNGG 2 cut(s) 44, 273
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 50, 377, 419
Bse3DI GCAATG 1 cut(s) 367
BseAI TCCGGA 1 cut(s) 157
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 44, 273
BseLI CCNNNNNNNGG 4 cut(s) 45, 50, 377, 419
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 2 cut(s) 544, 552
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 1 cut(s) 221
Bsh1236I CGCG 2 cut(s) 469, 505
BshFI GGCC 6 cut(s) 72, 175, 319, 382, 399, 463
BshNI GGYRCC 1 cut(s) 366
BshVI ATCGAT 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 548
BsiSI CCGG 3 cut(s) 158, 311, 383
BslI CCNNNNNNNGG 4 cut(s) 45, 50, 377, 419
BsmAI GTCTC 3 cut(s) 283, 528, 547
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 6 cut(s) 72, 175, 319, 382, 399, 463
Bso31I GGTCTC 1 cut(s) 283
Bsp119I TTCGAA 1 cut(s) 225
Bsp1286I GDGCHC 2 cut(s) 369, 548
Bsp13I TCCGGA 1 cut(s) 157
Bsp143I GATC 1 cut(s) 110
BspACI CCGC 4 cut(s) 3, 51, 349, 467
BspANI GGCC 6 cut(s) 72, 175, 319, 382, 399, 463
BspCNI CTCAG 2 cut(s) 543, 551
BspDI ATCGAT 1 cut(s) 84
BspEI TCCGGA 1 cut(s) 157
BspFNI CGCG 2 cut(s) 469, 505
BspLI GGNNCC 2 cut(s) 139, 368
BspPI GGATC 1 cut(s) 118
BspT104I TTCGAA 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 366
BspTNI GGTCTC 1 cut(s) 283
BsrDI GCAATG 1 cut(s) 367
BssECI CCNNGG 2 cut(s) 44, 273
BssMI GATC 1 cut(s) 110
BssT1I CCWWGG 2 cut(s) 44, 273
Bst4CI ACNGT 1 cut(s) 25
BstBAI YACGTR 1 cut(s) 192
BstBI TTCGAA 1 cut(s) 225
BstC8I GCNNGC 5 cut(s) 243, 247, 321, 425, 471
BstDEI CTNAG 3 cut(s) 487, 530, 538
BstFNI CGCG 2 cut(s) 469, 505
BstKTI GATC 1 cut(s) 113
BstMAI GTCTC 3 cut(s) 283, 528, 547
BstMBI GATC 1 cut(s) 110
BstMWI GCNNNNNNNGC 3 cut(s) 320, 345, 357
BstNSI RCATGY 1 cut(s) 249
BstPAI GACNNNNGTC 1 cut(s) 275
BstSCI CCNGG 1 cut(s) 382
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 2 cut(s) 469, 505
BstV1I GCAGC 1 cut(s) 221
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 6 cut(s) 72, 175, 319, 382, 399, 463
BsuTUI ATCGAT 1 cut(s) 84
Cac8I GCNNGC 5 cut(s) 243, 247, 321, 425, 471
Cfr13I GGNCC 3 cut(s) 270, 380, 397
ClaI ATCGAT 1 cut(s) 84
CseI GACGC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 193, 521
CviAII CATG 6 cut(s) 122, 206, 246, 250, 315, 356
CviQI GTAC 2 cut(s) 193, 521
DdeI CTNAG 3 cut(s) 487, 530, 538
DpnI GATC 1 cut(s) 112
DpnII GATC 1 cut(s) 110
EaeI YGGCCR 3 cut(s) 70, 173, 317
Ecl136II GAGCTC 1 cut(s) 546
Eco130I CCWWGG 2 cut(s) 44, 273
Eco147I AGGCCT 1 cut(s) 463
Eco24I GRGCYC 1 cut(s) 548
Eco31I GGTCTC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 270
Eco53kI GAGCTC 1 cut(s) 546
Eco57I CTGAAG 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 546
EcoT14I CCWWGG 2 cut(s) 44, 273
EcoT22I ATGCAT 1 cut(s) 251
EcoT38I GRGCYC 1 cut(s) 548
ErhI CCWWGG 2 cut(s) 44, 273
FaeI CATG 6 cut(s) 125, 209, 249, 253, 318, 359
FatI CATG 6 cut(s) 121, 205, 245, 249, 314, 355
Fnu4HI GCNGC 2 cut(s) 210, 349
FriOI GRGCYC 1 cut(s) 548
Fsp4HI GCNGC 2 cut(s) 210, 349
FspBI CTAG 1 cut(s) 557
GluI GCNGC 2 cut(s) 210, 349
HaeIII GGCC 6 cut(s) 72, 175, 319, 382, 399, 463
HapII CCGG 3 cut(s) 158, 311, 383
HgaI GACGC 1 cut(s) 170
Hin1II CATG 6 cut(s) 125, 209, 249, 253, 318, 359
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 3 cut(s) 14, 86, 536
HpaII CCGG 3 cut(s) 158, 311, 383
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 4 cut(s) 19, 168, 533, 541
Hpy188III TCNNGA 2 cut(s) 158, 552
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 1 cut(s) 568
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 2 cut(s) 191, 290
HpyCH4V TGCA 3 cut(s) 209, 241, 249
HpyF10VI GCNNNNNNNGC 3 cut(s) 320, 345, 357
HpyF3I CTNAG 3 cut(s) 487, 530, 538
HpySE526I ACGT 2 cut(s) 191, 290
Hsp92II CATG 6 cut(s) 125, 209, 249, 253, 318, 359
Kpn2I TCCGGA 1 cut(s) 157
Kzo9I GATC 1 cut(s) 110
LmnI GCTCC 1 cut(s) 453
LpnPI CCDG 7 cut(s) 72, 171, 324, 333, 396, 409, 445
Lsp1109I GCAGC 1 cut(s) 221
LweI GCATC 2 cut(s) 228, 427
MaeI CTAG 1 cut(s) 557
MaeII ACGT 2 cut(s) 191, 290
MaeIII GTNAC 1 cut(s) 499
MalI GATC 1 cut(s) 112
MboI GATC 1 cut(s) 110
MhlI GDGCHC 2 cut(s) 369, 548
MlsI TGGCCA 3 cut(s) 72, 175, 319
MluCI AATT 6 cut(s) 64, 76, 197, 221, 284, 448
MluNI TGGCCA 3 cut(s) 72, 175, 319
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 367
MnlI CCTC 1 cut(s) 474
Mox20I TGGCCA 3 cut(s) 72, 175, 319
Mph1103I ATGCAT 1 cut(s) 251
MroI TCCGGA 1 cut(s) 157
MscI TGGCCA 3 cut(s) 72, 175, 319
MslI CAYNNNNRTG 2 cut(s) 360, 435
Msp20I TGGCCA 3 cut(s) 72, 175, 319
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 3 cut(s) 158, 311, 383
MspR9I CCNGG 1 cut(s) 384
Mva1269I GAATGC 1 cut(s) 257
MvnI CGCG 2 cut(s) 469, 505
MwoI GCNNNNNNNGC 3 cut(s) 320, 345, 357
NciI CCSGG 1 cut(s) 384
NdeII GATC 1 cut(s) 110
NlaIII CATG 6 cut(s) 125, 209, 249, 253, 318, 359
NlaIV GGNNCC 2 cut(s) 139, 368
NsiI ATGCAT 1 cut(s) 251
NspI RCATGY 1 cut(s) 249
NspV TTCGAA 1 cut(s) 225
PaeI GCATGC 1 cut(s) 249
PceI AGGCCT 1 cut(s) 463
PctI GAATGC 1 cut(s) 257
PfeI GAWTC 2 cut(s) 14, 86
PkrI GCNGC 2 cut(s) 211, 350
PleI GAGTC 1 cut(s) 530
PpsI GAGTC 1 cut(s) 530
Ppu21I YACGTR 1 cut(s) 192
PshAI GACNNNNGTC 1 cut(s) 275
Psp124BI GAGCTC 1 cut(s) 548
PspN4I GGNNCC 2 cut(s) 139, 368
PspPI GGNCC 3 cut(s) 270, 380, 397
PvuII CAGCTG 1 cut(s) 323
RsaI GTAC 2 cut(s) 194, 522
RsaNI GTAC 2 cut(s) 193, 521
RseI CAYNNNNRTG 2 cut(s) 360, 435
SacI GAGCTC 1 cut(s) 548
SatI GCNGC 2 cut(s) 210, 349
Sau3AI GATC 1 cut(s) 110
Sau96I GGNCC 3 cut(s) 270, 380, 397
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 1 cut(s) 384
SduI GDGCHC 2 cut(s) 369, 548
SfaNI GCATC 2 cut(s) 228, 427
SfuI TTCGAA 1 cut(s) 225
SinI GGWCC 1 cut(s) 270
SmiMI CAYNNNNRTG 2 cut(s) 360, 435
SphI GCATGC 1 cut(s) 249
Sse9I AATT 6 cut(s) 64, 76, 197, 221, 284, 448
SseBI AGGCCT 1 cut(s) 463
SsiI CCGC 4 cut(s) 3, 51, 349, 467
SspMI CTAG 1 cut(s) 557
SstI GAGCTC 1 cut(s) 548
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 1 cut(s) 382
StyI CCWWGG 2 cut(s) 44, 273
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 2 cut(s) 194, 293
TaqI TCGA 4 cut(s) 84, 225, 332, 551
TasI AATT 6 cut(s) 64, 76, 197, 221, 284, 448
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 2 cut(s) 14, 86
TseI GCWGC 1 cut(s) 209
TspDTI ATGAA 4 cut(s) 108, 119, 419, 459
TspGWI ACGGA 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 270
XapI RAATTY 4 cut(s) 76, 197, 221, 284
XceI RCATGY 1 cut(s) 249
XspI CTAG 1 cut(s) 557
Zsp2I ATGCAT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.