FvH4_2g14090

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009280
Physical Location & Seq
Forward (+)
12392185 .. 12395373
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14090.t1

Sequence Viewer

Length: 1209 bp
ATGCTGTATCTCCTAATCTCTTGTGTCTCCTTCATTCTTGTTTCCAAGTCCATGACTAACAAGCAACCATGGCAGGAGGGTCAGCTCAAGTTGTTCTCAATTGGGTTATCAACTCTCCTAATGAACTGGTTCCAGAAGAGTAGGCTTCATACAGGTTTATTGCAACCCATTAGCTTCAGAGTTAAACACATGGTGATGAAGAGTTCAGGTTCAAGAGAAGTTTTGGAGAATGTGGATCAGGAGCAACCAGGTGGTCATGTTTGTCTATTGGACTTGCCTGATTTGACAATGGAGTGCATTCTTGAGAAGCTTTCCCCATCTGGGTTGTGCAGTATGGCTGGAGTTTGTACTTCTATGAGGGATAGGTGCATACGTGACCATTTTTGGGAGAAGCATATGAAGCAGAAATGGGGTAAACTGATTGGGGCTGCTGCTTATAGAGAATGGGAGTTGCAGCATACGGATTCAGCAAGAAGAAGAAACAACCAGTCTGATCAAACCAAGAAGAAAAGTGTATTTGACTCCCTTTCTGGGTTGTTGCAGTTTTGTTCATGGGTGATCAGACCAAAATTACAAAGCTGCAGGGTGAAGACTACTAGTGCTACTACTTTGCCGGTTGATTCTGTCATGTCTTTATATCTTTCTCTTGAAATAGGGGACTTCTGGTTCCCAGCTCAGGTCTATAACCGTGAGAATGGTAATGTAGGCTTTATGTTGTCTTGTTATGATGCACAATTGAGATACGATTCGGGATCAGACACTTTTCAAGCAAGGTATTCACCTTATGGGAGGAGGACAATGGAGGAAAATATACCATGGGATAGAATAAGAGCTGCTCCAGTTGATACTCCCTCACATATGCTTCATGTCTCTGATTGTTTACATGACTTAAAACCTGGTGATCATATCGAAATCCAATGGAGAAGAAGCAAGGAATTCCCTTATGGTTGGTGGTATGGTGTCATTGGCCACTTCGAGTTGTGCAACGGGAGTGAGAATCATTGCCATTGTCACTACAATGATACAGTAGTACTGGAGTTCAGTCAATATACCCCTGGCTCGCGGTGGAGGCAAATGATGATAAACCGAAAATACCACCGAGAAGAGGGGAACGAGGCAGATGGTTTCTATGGAGGAATTAGAAAGCTTTACAATGTAGAAGAAATTGCAAAGTGGAAGCTCCTATGGCCTAACCAAGTCCTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

403

Amino Acids

46.77

Weight (kDa)

8.34

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 89 - 131 9.1e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1201
AccII CGCG 1 cut(s) 1063
AciI CCGC 1 cut(s) 1063
AclWI GGATC 2 cut(s) 243, 760
AcoI YGGCCR 1 cut(s) 967
AcsI RAATTY 1 cut(s) 935
AcuI CTGAAG 1 cut(s) 160
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 2 cut(s) 349, 1032
AfiI CCNNNNNNNGG 6 cut(s) 321, 385, 531, 676, 1105, 1201
AgsI TTSAA 3 cut(s) 213, 650, 767
AhlI ACTAGT 1 cut(s) 596
AjnI CCWGG 4 cut(s) 247, 895, 1054, 1200
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 8 cut(s) 85, 174, 310, 579, 674, 833, 1147, 1180
AluI AGCT 8 cut(s) 85, 174, 310, 579, 674, 833, 1147, 1180
Alw26I GTCTC 2 cut(s) 31, 874
AlwI GGATC 2 cut(s) 243, 760
AoxI GGCC 2 cut(s) 967, 1187
ApeKI GCWGC 5 cut(s) 428, 431, 454, 579, 833
ApoI RAATTY 1 cut(s) 935
Asp700I GAANNNNTTC 1 cut(s) 128
AsuHPI GGTGA 5 cut(s) 205, 568, 598, 771, 911
BaeI ACNNNNGTAYC 2 cut(s) 1014, 1047
BalI TGGCCA 1 cut(s) 969
BbsI GAAGAC 1 cut(s) 596
BbvI GCAGC 5 cut(s) 415, 418, 466, 566, 820
BccI CCATC 2 cut(s) 325, 1115
BciT130I CCWGG 4 cut(s) 249, 897, 1056, 1202
BclI TGATCA 3 cut(s) 493, 558, 901
BcoDI GTCTC 2 cut(s) 31, 874
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 1 cut(s) 597
BfmI CTRYAG 1 cut(s) 580
BisI GCNGC 5 cut(s) 429, 432, 455, 580, 834
BlsI GCNGC 5 cut(s) 430, 433, 456, 581, 835
BmcAI AGTACT 1 cut(s) 1032
Bme1390I CCNGG 4 cut(s) 249, 897, 1056, 1202
BmiI GGNNCC 2 cut(s) 131, 668
BmrFI CCNGG 4 cut(s) 249, 897, 1056, 1202
BmsI GCATC 1 cut(s) 718
BpiI GAAGAC 1 cut(s) 596
BpmI CTGGAG 3 cut(s) 360, 822, 1055
Bpu10I CCTNAGC 1 cut(s) 675
BpuEI CTTGAG 2 cut(s) 71, 323
BsaAI YACGTR 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 68, 815, 1054
Bsc4I CCNNNNNNNGG 6 cut(s) 321, 385, 531, 676, 1105, 1201
Bse118I RCCGGY 1 cut(s) 613
Bse1I ACTGG 4 cut(s) 131, 487, 839, 1038
Bse3DI GCAATG 1 cut(s) 1000
BseBI CCWGG 4 cut(s) 249, 897, 1056, 1202
BseDI CCNNGG 3 cut(s) 68, 815, 1054
BseLI CCNNNNNNNGG 6 cut(s) 321, 385, 531, 676, 1105, 1201
BseMI GCAATG 1 cut(s) 1000
BseMII CTCAG 1 cut(s) 689
BseNI ACTGG 4 cut(s) 131, 487, 839, 1038
BseRI GAGGAG 1 cut(s) 805
BseXI GCAGC 5 cut(s) 415, 418, 466, 566, 820
BseYI CCCAGC 1 cut(s) 670
BsgI GTGCAG 1 cut(s) 349
Bsh1236I CGCG 1 cut(s) 1063
BshFI GGCC 2 cut(s) 969, 1189
BsiSI CCGG 1 cut(s) 614
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 6 cut(s) 321, 385, 531, 676, 1105, 1201
BsmAI GTCTC 2 cut(s) 31, 874
BsmFI GGGAC 1 cut(s) 671
BsmI GAATGC 1 cut(s) 297
BsnI GGCC 2 cut(s) 969, 1189
Bsp143I GATC 5 cut(s) 235, 493, 558, 752, 901
Bsp19I CCATGG 2 cut(s) 68, 815
BspACI CCGC 1 cut(s) 1063
BspANI GGCC 2 cut(s) 969, 1189
BspCNI CTCAG 1 cut(s) 688
BspFNI CGCG 1 cut(s) 1063
BspLI GGNNCC 2 cut(s) 131, 668
BspMAI CTGCAG 1 cut(s) 584
BspPI GGATC 2 cut(s) 243, 760
BsrDI GCAATG 1 cut(s) 1000
BsrFI RCCGGY 1 cut(s) 613
BsrI ACTGG 4 cut(s) 131, 487, 839, 1038
BssAI RCCGGY 1 cut(s) 613
BssECI CCNNGG 3 cut(s) 68, 815, 1054
BssMI GATC 5 cut(s) 235, 493, 558, 752, 901
BssT1I CCWWGG 2 cut(s) 68, 815
Bst2UI CCWGG 4 cut(s) 249, 897, 1056, 1202
Bst4CI ACNGT 2 cut(s) 689, 1027
Bst6I CTCTTC 3 cut(s) 131, 194, 1098
BstBAI YACGTR 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 1061
BstDEI CTNAG 1 cut(s) 675
BstDSI CCRYGG 2 cut(s) 68, 815
BstFNI CGCG 1 cut(s) 1063
BstKTI GATC 5 cut(s) 238, 496, 561, 755, 904
BstMAI GTCTC 2 cut(s) 31, 874
BstMBI GATC 5 cut(s) 235, 493, 558, 752, 901
BstMWI GCNNNNNNNGC 4 cut(s) 70, 400, 1069, 1186
BstNI CCWGG 4 cut(s) 249, 897, 1056, 1202
BstSCI CCNGG 4 cut(s) 247, 895, 1054, 1200
BstSFI CTRYAG 1 cut(s) 580
BstUI CGCG 1 cut(s) 1063
BstV1I GCAGC 5 cut(s) 415, 418, 466, 566, 820
BstV2I GAAGAC 1 cut(s) 596
BsuRI GGCC 2 cut(s) 969, 1189
BtgI CCRYGG 2 cut(s) 68, 815
Cac8I GCNNGC 1 cut(s) 1061
Cfr10I RCCGGY 1 cut(s) 613
CsiI ACCWGGT 2 cut(s) 247, 895
Csp6I GTAC 2 cut(s) 348, 1031
CviAII CATG 9 cut(s) 52, 69, 190, 257, 552, 628, 816, 866, 884
CviQI GTAC 2 cut(s) 348, 1031
DdeI CTNAG 1 cut(s) 675
DpnI GATC 5 cut(s) 237, 495, 560, 754, 903
DpnII GATC 5 cut(s) 235, 493, 558, 752, 901
DraIII CACNNNGTG 1 cut(s) 193
EaeI YGGCCR 1 cut(s) 967
Eam1104I CTCTTC 3 cut(s) 131, 194, 1098
EarI CTCTTC 3 cut(s) 131, 194, 1098
Eco130I CCWWGG 2 cut(s) 68, 815
Eco57I CTGAAG 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 935
EcoRII CCWGG 4 cut(s) 247, 895, 1054, 1200
EcoT14I CCWWGG 2 cut(s) 68, 815
ErhI CCWWGG 2 cut(s) 68, 815
FaeI CATG 9 cut(s) 55, 72, 193, 260, 555, 631, 819, 869, 887
FaqI GGGAC 1 cut(s) 671
FatI CATG 9 cut(s) 51, 68, 189, 256, 551, 627, 815, 865, 883
FauNDI CATATG 2 cut(s) 396, 858
FbaI TGATCA 3 cut(s) 493, 558, 901
Fnu4HI GCNGC 5 cut(s) 429, 432, 455, 580, 834
Fsp4HI GCNGC 5 cut(s) 429, 432, 455, 580, 834
FspBI CTAG 1 cut(s) 597
GluI GCNGC 5 cut(s) 429, 432, 455, 580, 834
GsaI CCCAGC 1 cut(s) 674
GsuI CTGGAG 3 cut(s) 360, 822, 1055
HaeIII GGCC 2 cut(s) 969, 1189
HapII CCGG 1 cut(s) 614
Hin1II CATG 9 cut(s) 55, 72, 193, 260, 555, 631, 819, 869, 887
HindIII AAGCTT 2 cut(s) 308, 1145
HinfI GANTC 5 cut(s) 464, 521, 620, 746, 997
HpaII CCGG 1 cut(s) 614
HphI GGTGA 5 cut(s) 205, 568, 598, 771, 911
Hpy166II GTNNAC 2 cut(s) 416, 881
Hpy188I TCNGA 5 cut(s) 179, 493, 563, 757, 874
Hpy188III TCNNGA 6 cut(s) 133, 213, 239, 302, 647, 750
Hpy8I GTNNAC 2 cut(s) 416, 881
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 2 cut(s) 689, 1027
HpyCH4IV ACGT 1 cut(s) 373
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 400, 1069, 1186
HpyF3I CTNAG 1 cut(s) 675
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 9 cut(s) 55, 72, 193, 260, 555, 631, 819, 869, 887
Ksp22I TGATCA 3 cut(s) 493, 558, 901
Kzo9I GATC 5 cut(s) 235, 493, 558, 752, 901
LmnI GCTCC 3 cut(s) 241, 841, 1185
Lsp1109I GCAGC 5 cut(s) 415, 418, 466, 566, 820
LweI GCATC 1 cut(s) 718
MabI ACCWGGT 2 cut(s) 247, 895
MaeI CTAG 1 cut(s) 597
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 2 cut(s) 374, 1010
MalI GATC 5 cut(s) 237, 495, 560, 754, 903
MboI GATC 5 cut(s) 235, 493, 558, 752, 901
MboII GAAGA 9 cut(s) 148, 211, 486, 489, 517, 601, 936, 1115, 1172
MfeI CAATTG 2 cut(s) 99, 734
MlsI TGGCCA 1 cut(s) 969
MluCI AATT 6 cut(s) 99, 569, 734, 935, 1137, 1164
MluNI TGGCCA 1 cut(s) 969
MlyI GAGTC 1 cut(s) 515
Mox20I TGGCCA 1 cut(s) 969
MroXI GAANNNNTTC 1 cut(s) 128
MscI TGGCCA 1 cut(s) 969
MseI TTAA 2 cut(s) 183, 890
MslI CAYNNNNRTG 2 cut(s) 194, 1017
Msp20I TGGCCA 1 cut(s) 969
MspI CCGG 1 cut(s) 614
MspR9I CCNGG 4 cut(s) 249, 897, 1056, 1202
MunI CAATTG 2 cut(s) 99, 734
Mva1269I GAATGC 1 cut(s) 297
MvaI CCWGG 4 cut(s) 249, 897, 1056, 1202
MvnI CGCG 1 cut(s) 1063
MwoI GCNNNNNNNGC 4 cut(s) 70, 400, 1069, 1186
NcoI CCATGG 2 cut(s) 68, 815
NdeI CATATG 2 cut(s) 396, 858
NdeII GATC 5 cut(s) 235, 493, 558, 752, 901
NlaIII CATG 9 cut(s) 55, 72, 193, 260, 555, 631, 819, 869, 887
NlaIV GGNNCC 2 cut(s) 131, 668
NmuCI GTSAC 2 cut(s) 374, 1010
PctI GAATGC 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 4 cut(s) 464, 620, 746, 997
PflMI CCANNNNNTGG 1 cut(s) 1201
PfoI TCCNGGA 1 cut(s) 1200
PkrI GCNGC 5 cut(s) 430, 433, 456, 581, 835
PleI GAGTC 1 cut(s) 515
PpsI GAGTC 1 cut(s) 515
Ppu21I YACGTR 1 cut(s) 374
Psp6I CCWGG 4 cut(s) 247, 895, 1054, 1200
PspFI CCCAGC 1 cut(s) 670
PspGI CCWGG 4 cut(s) 247, 895, 1054, 1200
PspN4I GGNNCC 2 cut(s) 131, 668
PstI CTGCAG 1 cut(s) 584
RsaI GTAC 2 cut(s) 349, 1032
RsaNI GTAC 2 cut(s) 348, 1031
RseI CAYNNNNRTG 2 cut(s) 194, 1017
SaqAI TTAA 2 cut(s) 183, 890
SatI GCNGC 5 cut(s) 429, 432, 455, 580, 834
Sau3AI GATC 5 cut(s) 235, 493, 558, 752, 901
ScaI AGTACT 1 cut(s) 1032
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 4 cut(s) 249, 897, 1056, 1202
SexAI ACCWGGT 2 cut(s) 247, 895
SfaNI GCATC 1 cut(s) 718
SfcI CTRYAG 1 cut(s) 580
SmiMI CAYNNNNRTG 2 cut(s) 194, 1017
SmlI CTYRAG 2 cut(s) 86, 302
SmoI CTYRAG 2 cut(s) 86, 302
SpeI ACTAGT 1 cut(s) 596
Sse9I AATT 6 cut(s) 99, 569, 734, 935, 1137, 1164
SsiI CCGC 1 cut(s) 1063
SspMI CTAG 1 cut(s) 597
StyD4I CCNGG 4 cut(s) 247, 895, 1054, 1200
StyI CCWWGG 2 cut(s) 68, 815
TaaI ACNGT 2 cut(s) 689, 1027
TaiI ACGT 1 cut(s) 376
TaqI TCGA 2 cut(s) 909, 975
TasI AATT 6 cut(s) 99, 569, 734, 935, 1137, 1164
TatI WGTACW 2 cut(s) 347, 1030
TfiI GAWTC 4 cut(s) 464, 620, 746, 997
Tru1I TTAA 2 cut(s) 183, 890
Tru9I TTAA 2 cut(s) 183, 890
TseFI GTSAC 2 cut(s) 374, 1010
TseI GCWGC 5 cut(s) 428, 431, 454, 579, 833
Tsp45I GTSAC 2 cut(s) 374, 1010
TspDTI ATGAA 7 cut(s) 22, 137, 137, 212, 413, 540, 854
TspGWI ACGGA 1 cut(s) 476
Van91I CCANNNNNTGG 1 cut(s) 1201
XapI RAATTY 1 cut(s) 935
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 1 cut(s) 597
ZrmI AGTACT 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.