pycom12g08510

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009280
Physical Location & Seq
Forward (+)
9726881 .. 9729720
2840 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g08510.1

Sequence Viewer

Length: 1200 bp
ATGCTTTGCCTCCTTATCTCTTGTGCCTCTTTCATCCTCCTTTCTAAGTCCTTCATTCACCACAAACCAATATTACAAACATGGGAGGATAGCCTCAGATTAATCTCACTCAAGTTTTTGGGTGGATTTTTAAGTTTTCTCCACAAAAGCAGGATTTCACTCCAACCCATCACCTCCAAATTCAAAAAGATGGTGACTTTTGGCTCAAATGTGGAGGTGGAGAGAGTGGAGAGAGAGGAGAGAGAAACCAGTCTCTCTCTCCTGGATTTGCCTTACTTGGCCTTGGACTGCATTCTTGAGAGGCTTCCCCCATCTGGGTTGTGCTCCATGGCAGCAGTTTGTGCTTCTTTGAGGGAGAGCTGCACAAGTGATCACTTGTGGGGGAACCATATGAACCACAAATGGGGTCGACTTATAGGGCCTGCCGCTTACAGAGAGTGGCAGTTGCATGTGGCCTCAAGAAACAGAATCAGAGATTACTCTGTTCGGACCGAGAAAAAAGGTCTAATAGAGTCCCTTGTGGCTAGTATTTTGCCTTCCTCGTGGATTACTAGGCAAAAAATAACTTCTGTATGTAAAGTGAAGGCTAGTACGAGTTGCTTACCGGTCGATTCGATCATGTCTCTCTATCTCTCTCTTGAAACTGGCAAGTTTTGGTTCCCGGCTCAGGTCTATAACCGCGAGAACGGTAATGTAGGGTTTGTGCTGTCTTGTTATGATGCACAAGTGCGCTATGATTCGACGACAAACACATTTCAAGCAAGGTATTCACCTTACGGGCGGAGGGCAACAGAGGAAAATATACCATGGGATAGACTAAGAGCGGCTCCAGTTGATACTCCTCCTCACAAACTTCATGTCTCCGATTGTTTACATGACTTAAAACCCGATGACCATATTGAGATCCAGTGGAGAAGAAACAAAGAATTTCCTTATGGTTGGTGGTATGGTGTCATTGGTCACTTAGAGTTTTGCGATGGGAATGAGAATCGCTGCCGTTGTCACTGCAATGATATAGTAAAACTGGAGTTCAGTCAGTATGCTCCTGGTTCCCGATGGAGAGAAATGACGATAAACAGGAAGGATCATCGCGAAGAAGGCAATGAAGCAGACGGCTTCTATGCAGGAATCCGAAAGATTTACAACGAGGAGGAAATTGCAAGGTGGAAGCGCTTATGGCCGACCCAGGTTCTGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

400

Amino Acids

46.24

Weight (kDa)

8.67

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 87 - 127 2.4e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1192
AccBSI CCGCTC 1 cut(s) 824
AccI GTMKAC 1 cut(s) 409
AccII CGCG 2 cut(s) 681, 1092
AciI CCGC 4 cut(s) 426, 679, 781, 824
AclWI GGATC 2 cut(s) 898, 1092
AcoI YGGCCR 1 cut(s) 1178
AcsI RAATTY 2 cut(s) 179, 926
AfaI GTAC 1 cut(s) 592
AfeI AGCGCT 1 cut(s) 1172
AfiI CCNNNNNNNGG 5 cut(s) 314, 315, 403, 667, 1192
AgeI ACCGGT 1 cut(s) 604
AgsI TTSAA 3 cut(s) 184, 641, 758
AjnI CCWGG 3 cut(s) 261, 1045, 1185
AluBI AGCT 1 cut(s) 360
AluI AGCT 1 cut(s) 360
Alw21I GWGCWC 1 cut(s) 326
Alw26I GTCTC 3 cut(s) 257, 627, 865
AlwI GGATC 2 cut(s) 898, 1092
AlwNI CAGNNNCTG 1 cut(s) 1192
Aor51HI AGCGCT 1 cut(s) 1172
AoxI GGCC 4 cut(s) 279, 419, 453, 1178
ApeKI GCWGC 3 cut(s) 332, 360, 993
ApoI RAATTY 2 cut(s) 179, 926
ArsI GACNNNNNNTTYG 2 cut(s) 843, 875
AseI ATTAAT 1 cut(s) 101
AsiGI ACCGGT 1 cut(s) 604
AspLEI GCGC 2 cut(s) 732, 1173
AspS9I GGNCC 2 cut(s) 419, 489
AsuC2I CCSGG 1 cut(s) 662
AsuHPI GGTGA 4 cut(s) 50, 163, 205, 762
AvaII GGWCC 1 cut(s) 489
BarI GAAGNNNNNNTAC 2 cut(s) 520, 552
BauI CACGAG 1 cut(s) 541
Bbv12I GWGCWC 1 cut(s) 326
BbvI GCAGC 3 cut(s) 344, 347, 980
BccI CCATC 5 cut(s) 176, 184, 319, 971, 1050
BceAI ACGGC 2 cut(s) 981, 1129
BciT130I CCWGG 3 cut(s) 263, 1047, 1187
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 1 cut(s) 662
BcoDI GTCTC 3 cut(s) 257, 627, 865
BfaI CTAG 3 cut(s) 525, 552, 588
BfoI RGCGCY 1 cut(s) 1174
BisI GCNGC 5 cut(s) 333, 361, 426, 825, 994
BlsI GCNGC 5 cut(s) 334, 362, 427, 826, 995
Bme1390I CCNGG 4 cut(s) 263, 662, 1047, 1187
Bme18I GGWCC 1 cut(s) 489
BmgT120I GGNCC 2 cut(s) 419, 489
BmiI GGNNCC 4 cut(s) 386, 659, 828, 1051
BmrFI CCNGG 4 cut(s) 263, 662, 1047, 1187
BmsI GCATC 1 cut(s) 709
BpmI CTGGAG 2 cut(s) 813, 1046
Bpu10I CCTNAGC 1 cut(s) 666
BpuEI CTTGAG 3 cut(s) 95, 317, 442
BpuMI CCSGG 1 cut(s) 662
BsaJI CCNNGG 4 cut(s) 282, 327, 806, 1185
BsaWI WCCGGW 1 cut(s) 604
Bsc4I CCNNNNNNNGG 5 cut(s) 314, 315, 403, 667, 1192
Bse118I RCCGGY 1 cut(s) 604
Bse1I ACTGG 5 cut(s) 249, 649, 830, 907, 1029
Bse3DI GCAATG 2 cut(s) 1015, 1108
BseBI CCWGG 3 cut(s) 263, 1047, 1187
BseDI CCNNGG 4 cut(s) 282, 327, 806, 1185
BseGI GGATG 1 cut(s) 33
BseLI CCNNNNNNNGG 5 cut(s) 314, 315, 403, 667, 1192
BseMI GCAATG 2 cut(s) 1015, 1108
BseMII CTCAG 2 cut(s) 109, 680
BseNI ACTGG 5 cut(s) 249, 649, 830, 907, 1029
BseRI GAGGAG 4 cut(s) 251, 831, 834, 1163
BseXI GCAGC 3 cut(s) 344, 347, 980
BsgI GTGCAG 1 cut(s) 346
Bsh1236I CGCG 2 cut(s) 681, 1092
Bsh1285I CGRYCG 1 cut(s) 609
BshFI GGCC 4 cut(s) 281, 421, 455, 1180
BshTI ACCGGT 1 cut(s) 604
BsiEI CGRYCG 1 cut(s) 609
BsiHKAI GWGCWC 1 cut(s) 326
BsiSI CCGG 2 cut(s) 605, 662
BslFI GGGAC 1 cut(s) 499
BslI CCNNNNNNNGG 5 cut(s) 314, 315, 403, 667, 1192
BsmAI GTCTC 3 cut(s) 257, 627, 865
BsmFI GGGAC 1 cut(s) 499
BsmI GAATGC 1 cut(s) 291
BsnI GGCC 4 cut(s) 281, 421, 455, 1180
Bsp1286I GDGCHC 1 cut(s) 326
Bsp143I GATC 4 cut(s) 370, 615, 903, 1084
Bsp19I CCATGG 2 cut(s) 327, 806
Bsp68I TCGCGA 1 cut(s) 1092
BspACI CCGC 4 cut(s) 426, 679, 781, 824
BspANI GGCC 4 cut(s) 281, 421, 455, 1180
BspCNI CTCAG 2 cut(s) 108, 679
BspFNI CGCG 2 cut(s) 681, 1092
BspLI GGNNCC 4 cut(s) 386, 659, 828, 1051
BspPI GGATC 2 cut(s) 898, 1092
BsrBI CCGCTC 1 cut(s) 824
BsrDI GCAATG 2 cut(s) 1015, 1108
BsrFI RCCGGY 1 cut(s) 604
BsrI ACTGG 5 cut(s) 249, 649, 830, 907, 1029
BssAI RCCGGY 1 cut(s) 604
BssECI CCNNGG 4 cut(s) 282, 327, 806, 1185
BssMI GATC 4 cut(s) 370, 615, 903, 1084
BssSI CACGAG 1 cut(s) 541
BssT1I CCWWGG 3 cut(s) 282, 327, 806
Bst2BI CACGAG 1 cut(s) 541
Bst2UI CCWGG 3 cut(s) 263, 1047, 1187
Bst4CI ACNGT 1 cut(s) 689
BstAPI GCANNNNNTGC 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 5 cut(s) 45, 95, 666, 818, 964
BstDSI CCRYGG 2 cut(s) 327, 806
BstF5I GGATG 1 cut(s) 33
BstFNI CGCG 2 cut(s) 681, 1092
BstH2I RGCGCY 1 cut(s) 1174
BstHHI GCGC 2 cut(s) 732, 1173
BstKTI GATC 4 cut(s) 373, 618, 906, 1087
BstMAI GTCTC 3 cut(s) 257, 627, 865
BstMBI GATC 4 cut(s) 370, 615, 903, 1084
BstMCI CGRYCG 1 cut(s) 609
BstMWI GCNNNNNNNGC 3 cut(s) 341, 1098, 1177
BstNI CCWGG 3 cut(s) 263, 1047, 1187
BstNSI RCATGY 1 cut(s) 452
BstSCI CCNGG 4 cut(s) 261, 660, 1045, 1185
BstUI CGCG 2 cut(s) 681, 1092
BstV1I GCAGC 3 cut(s) 344, 347, 980
BstX2I RGATCY 1 cut(s) 903
BstYI RGATCY 1 cut(s) 903
BsuRI GGCC 4 cut(s) 281, 421, 455, 1180
BtgI CCRYGG 2 cut(s) 327, 806
BtgZI GCGATG 2 cut(s) 990, 1073
BtsCI GGATG 1 cut(s) 33
BtsI GCAGTG 1 cut(s) 1003
BtsIMutI CAGTG 2 cut(s) 914, 1003
BtuMI TCGCGA 1 cut(s) 1092
Cac8I GCNNGC 1 cut(s) 423
CaiI CAGNNNCTG 1 cut(s) 1192
CfoI GCGC 2 cut(s) 732, 1173
Cfr10I RCCGGY 1 cut(s) 604
Cfr13I GGNCC 2 cut(s) 419, 489
CpoI CGGWCCG 1 cut(s) 489
Csp6I GTAC 1 cut(s) 591
CspAI ACCGGT 1 cut(s) 604
CspI CGGWCCG 1 cut(s) 489
CviAII CATG 7 cut(s) 81, 328, 449, 619, 807, 857, 875
CviQI GTAC 1 cut(s) 591
DdeI CTNAG 5 cut(s) 45, 95, 666, 818, 964
DpnI GATC 4 cut(s) 372, 617, 905, 1086
DpnII GATC 4 cut(s) 370, 615, 903, 1084
EaeI YGGCCR 1 cut(s) 1178
EciI GGCGGA 1 cut(s) 796
Eco130I CCWWGG 3 cut(s) 282, 327, 806
Eco47I GGWCC 1 cut(s) 489
Eco47III AGCGCT 1 cut(s) 1172
EcoO109I RGGNCCY 1 cut(s) 419
EcoRII CCWGG 3 cut(s) 261, 1045, 1185
EcoT14I CCWWGG 3 cut(s) 282, 327, 806
ErhI CCWWGG 3 cut(s) 282, 327, 806
FaeI CATG 7 cut(s) 84, 331, 452, 622, 810, 860, 878
FaqI GGGAC 1 cut(s) 499
FatI CATG 7 cut(s) 80, 327, 448, 618, 806, 856, 874
FauNDI CATATG 1 cut(s) 390
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 409
Fnu4HI GCNGC 5 cut(s) 333, 361, 426, 825, 994
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 5 cut(s) 333, 361, 426, 825, 994
FspBI CTAG 3 cut(s) 525, 552, 588
GlaI GCGC 2 cut(s) 731, 1172
GluI GCNGC 5 cut(s) 333, 361, 426, 825, 994
GsuI CTGGAG 2 cut(s) 813, 1046
HaeII RGCGCY 1 cut(s) 1174
HaeIII GGCC 4 cut(s) 281, 421, 455, 1180
HapII CCGG 2 cut(s) 605, 662
HhaI GCGC 2 cut(s) 732, 1173
Hin1II CATG 7 cut(s) 84, 331, 452, 622, 810, 860, 878
Hin6I GCGC 2 cut(s) 730, 1171
HinP1I GCGC 2 cut(s) 730, 1171
HincII GTYRAC 1 cut(s) 410
HindII GTYRAC 1 cut(s) 410
HinfI GANTC 6 cut(s) 468, 512, 611, 737, 988, 1128
HpaII CCGG 2 cut(s) 605, 662
HphI GGTGA 4 cut(s) 50, 163, 205, 762
Hpy166II GTNNAC 2 cut(s) 410, 872
Hpy188I TCNGA 5 cut(s) 98, 473, 489, 865, 1133
Hpy188III TCNNGA 5 cut(s) 296, 459, 638, 1053, 1091
Hpy8I GTNNAC 2 cut(s) 410, 872
Hpy99I CGWCG 1 cut(s) 745
HpyAV CCTTC 5 cut(s) 61, 546, 577, 1075, 1091
HpyCH4III ACNGT 1 cut(s) 689
HpyCH4V TGCA 7 cut(s) 291, 363, 448, 722, 1008, 1124, 1160
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 1098, 1177
HpyF3I CTNAG 5 cut(s) 45, 95, 666, 818, 964
Hsp92II CATG 7 cut(s) 84, 331, 452, 622, 810, 860, 878
HspAI GCGC 2 cut(s) 730, 1171
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 370, 615, 903, 1084
LmnI GCTCC 3 cut(s) 329, 832, 1048
Lsp1109I GCAGC 3 cut(s) 344, 347, 980
LweI GCATC 1 cut(s) 709
MaeI CTAG 3 cut(s) 525, 552, 588
MaeIII GTNAC 3 cut(s) 193, 959, 1001
MalI GATC 4 cut(s) 372, 617, 905, 1086
MbiI CCGCTC 1 cut(s) 824
MboI GATC 4 cut(s) 370, 615, 903, 1084
MboII GAAGA 2 cut(s) 927, 1106
MflI RGATCY 1 cut(s) 903
MhlI GDGCHC 1 cut(s) 326
MluCI AATT 3 cut(s) 179, 926, 1155
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 187
MseI TTAA 3 cut(s) 101, 131, 881
MslI CAYNNNNRTG 1 cut(s) 1008
MspI CCGG 2 cut(s) 605, 662
MspR9I CCNGG 4 cut(s) 263, 662, 1047, 1187
Mva1269I GAATGC 1 cut(s) 291
MvaI CCWGG 3 cut(s) 263, 1047, 1187
MvnI CGCG 2 cut(s) 681, 1092
MwoI GCNNNNNNNGC 3 cut(s) 341, 1098, 1177
NciI CCSGG 1 cut(s) 662
NcoI CCATGG 2 cut(s) 327, 806
NdeI CATATG 1 cut(s) 390
NdeII GATC 4 cut(s) 370, 615, 903, 1084
NlaIII CATG 7 cut(s) 84, 331, 452, 622, 810, 860, 878
NlaIV GGNNCC 4 cut(s) 386, 659, 828, 1051
NmuCI GTSAC 3 cut(s) 193, 959, 1001
NruI TCGCGA 1 cut(s) 1092
NspI RCATGY 1 cut(s) 452
PctI GAATGC 1 cut(s) 291
PfeI GAWTC 5 cut(s) 468, 611, 737, 988, 1128
PflMI CCANNNNNTGG 1 cut(s) 1192
PfoI TCCNGGA 1 cut(s) 261
PinAI ACCGGT 1 cut(s) 604
PkrI GCNGC 5 cut(s) 334, 362, 427, 826, 995
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
PshBI ATTAAT 1 cut(s) 101
Psp6I CCWGG 3 cut(s) 261, 1045, 1185
PspGI CCWGG 3 cut(s) 261, 1045, 1185
PspN4I GGNNCC 4 cut(s) 386, 659, 828, 1051
PspPI GGNCC 2 cut(s) 419, 489
PstNI CAGNNNCTG 1 cut(s) 1192
PsuI RGATCY 1 cut(s) 903
RruI TCGCGA 1 cut(s) 1092
RsaI GTAC 1 cut(s) 592
RsaNI GTAC 1 cut(s) 591
RseI CAYNNNNRTG 1 cut(s) 1008
Rsr2I CGGWCCG 1 cut(s) 489
RsrII CGGWCCG 1 cut(s) 489
SalI GTCGAC 1 cut(s) 408
SaqAI TTAA 3 cut(s) 101, 131, 881
SatI GCNGC 5 cut(s) 333, 361, 426, 825, 994
Sau3AI GATC 4 cut(s) 370, 615, 903, 1084
Sau96I GGNCC 2 cut(s) 419, 489
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 4 cut(s) 263, 662, 1047, 1187
SduI GDGCHC 1 cut(s) 326
SetI ASST 9 cut(s) 176, 219, 362, 505, 672, 767, 775, 1166, 1191
SfaNI GCATC 1 cut(s) 709
SinI GGWCC 1 cut(s) 489
SmiMI CAYNNNNRTG 1 cut(s) 1008
SmlI CTYRAG 3 cut(s) 110, 296, 457
SmoI CTYRAG 3 cut(s) 110, 296, 457
Sse9I AATT 3 cut(s) 179, 926, 1155
SsiI CCGC 4 cut(s) 426, 679, 781, 824
SspI AATATT 1 cut(s) 72
SspMI CTAG 3 cut(s) 525, 552, 588
StyD4I CCNGG 4 cut(s) 261, 660, 1045, 1185
StyI CCWWGG 3 cut(s) 282, 327, 806
TaaI ACNGT 1 cut(s) 689
TaqI TCGA 4 cut(s) 409, 609, 614, 740
TaqII GACCGA 1 cut(s) 506
TasI AATT 3 cut(s) 179, 926, 1155
TauI GCSGC 2 cut(s) 428, 827
TfiI GAWTC 5 cut(s) 468, 611, 737, 988, 1128
Tru1I TTAA 3 cut(s) 101, 131, 881
Tru9I TTAA 3 cut(s) 101, 131, 881
TscAI CASTG 2 cut(s) 914, 1010
TseFI GTSAC 3 cut(s) 193, 959, 1001
TseI GCWGC 3 cut(s) 332, 360, 993
Tsp45I GTSAC 3 cut(s) 193, 959, 1001
TspDTI ATGAA 5 cut(s) 22, 43, 407, 845, 1119
TspRI CASTG 2 cut(s) 914, 1010
Van91I CCANNNNNTGG 1 cut(s) 1192
VpaK11BI GGWCC 1 cut(s) 489
VspI ATTAAT 1 cut(s) 101
XapI RAATTY 2 cut(s) 179, 926
XceI RCATGY 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 409
XspI CTAG 3 cut(s) 525, 552, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.