Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0003979

Physical Location & Seq

Genomic Coordinates Forward (+)

14626150 .. 14630678

Gene Structure 4529 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g16810.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2793 bp

ATGAATCGCATTTGCCTGTTGGCAATCCTCATCATCTGTGTTGGAAGATTCACAGAAGGAGCATCAAGACCTGCTGTTGTGAACATTGGAGCTATGTTTGCAGTAAGCACCATAAATGGAGGAGTGTCCAAGATTGCCATTAAGGCAGCTGAGGAGGATGTGAATGCCGATCCAAGCATTCTTAGCGGAACAAAATTTTCTGTATCTATTCATGATTCAAACTACAGTGGATTTCTCAGCATCATTGGAGTGGCTATAATTGGTCCCCAAACTTCTGTCATGGCTCATATAATCTCACATCTTGCAAATGAACTACACGTGCCGTTACTGTCATTCACTGCACTAGATCCCACTCTAACAAGCCTGCAGTACCCTTACTTTCTTCAAACTGCACCAAATGATCAATTCCAGATGAATGCTATTGGAGACATTGTTAGTTATTTTGGTTGGAAAGAGGTGGTTGCACTTTTTACTGATGATGATCAGAGCCGAAATGGTGTCACTGCATTAGGTGATAAGCTTGCAGAAAAAACGCACAAGATTTCTTACAAGGCAGTACTTCCACCTGATCCAACAGCAACTCGAGATCAAGTTAAGAATGAGTTGGTAAAGATTCAGATAATGGAGTCTCGAGTAATTGTTCTGCACACATTCTCGAGAACAGGCCTCTTGGTTTTTGATGTTGCAAAGGAACTTGGGATGATGGAGAGTGAATATGTCTGGATAGCTACTTCTTGGTTGTCCACAGTTTTGGATTCAAAATCCCCACTTCCTCAGAAGACTAAAGATTCCATCCAAGGAGTTCTCACTCTTCGTCCACACACACCGGATTCACAAAGGAAAAGAGCTTTCATTTCAAGGTGGAAGAAATTGAGTAATGGCACTATTGGGTTGAACCCTTATGGTCTATATGCCTACGACACTGTTTGGATTATTGCTCATGCTGTCAATTTGCTTTTGGATCAGGGAGGTACCATTTCCTTCTCCAAGCATACTAGTATACCTCGTTATGGGGGAGGCATTATGAACCTTTCTGCATTAAGCATTTTTGATGGTGGCCAGCAGTTGCTGGAAAACATATTGCAAACCAACACAACCGGTCTCACTGGTCCACTGGCTTTTCATTCAGACAGGTCTCCAGTGAATCCTTCATATGATATCATTAACATAATGGAAAATGGTTATCAACAGGTTGGATACTGGTATAACAATTCAGGGCTATCTGTTGTGCCCCCCAAAACACCATCTAACTGGTCTAGTTCAAACCAACATCTAGGTGTTGTTGTATGGCCTGGAGGAACAACAAAAAAGCCTCGAGGGTGGGTTTTTCCAAACAACGGGAAGCAGTTGAGAATTGGAGTACCAAATCGAGTAGGCTATCGTGCTTTTGTGTCACGACAAAATGGTACAGATGTAGTTAAAGGATACTGCATTGACATTTTCCTTGCAGCCATAAAATTGCTTCCCTATGCACTTCCACATAGGTTTGAACTGTTTGGTGATGGCCACAAGAATCCGAGCTACGATGAGCTTGTTAACATGGTTGCTTCAGGTAAATTTGATGCTGCTGTTGGTGACATTGCAATAGTTGCAAATCGAACAAAGACTGTGGATTTCAGTCAGCCATATATAGAGTCAGGGCTAGTAGTGGTGGCACCACTTAGGAGATCAAACTCAAGAGCTTGGGCATTTATGCAGCCGTTTTCTCCATTGATGTGGGGTATCACAGCGGCTTTCTTCCTAATTGTTGGATCAGTCCTGTGGATTCTTGAACATAGAATAAATGATGAGTTCCGTGGCCCTCCTAGGAAACAGATTGGCACAATTTTATGGTTTAGTTTCTCCACTATGTTTTTTGCTCATAGAGAAAATACTGTGAGCTTACTAGGGAGAATGGTGCTAATCATCTGGCTTTTTATAGTTCTGATAATCAATTCAAGCTACACAGCTAGCCTAACATCAATGCTCACAATACAACAATTATCGTCACCTATCACTGGCATTGATACCTTAATATCTAGCACTGAGCCTATAGGATTCCAAGTAGGGTCTTTTGCTCAAAACTATCTGATTGAGGAACTCAACATCCCAAACTCTAGACTGGTTCCTCTCGGTTCACCAGAAGAATATGCAAGAGCCCTTAAGAATAAAACTGTTGCTGCTGTGGTTGATGAAGGACCTTACATAGAACTCTTCCTCTCAGATAACTGCATGTTCTCAATCAGAGGGCCAGAATTCACCAAAAGTGGCTGGGGATTTGCATTTCCAAGAGACTCTCCTTTAGCCATAGACATGTCAACTGCCATTCTCACTCTATCAGAGAACGGAGAGCTTCAGCAAATTCATGAGAAATGGTTGTCAAAAAAGACTTGTGCGTCACAAACCTCTGACGACGTATCAGACCAGCTTCAACTACAAAGCTTCTGGGGACTGTTCCTAATATGTGGCACTGCATGTGTCATTGCTCTAGTCATTCATTTTTCATTGGCTTTTCGACAGTACCTCAGGCGTTCCCCTGAAGATGATCACCAATCTGATCTAGAGCCTTCTGGCCATGGCAGCACAAGTTATGGTACAACTGCAACACGCCGCCTCACATTCTTGTCATTCATTGATGAAAAGAAAGATCAATCAAAAGATAACAAGTCGAAAAGGAAACGCAAGGAAATAGCATCATCTAATAGGAATGGAAAGGAAGATGAATCAAGGGATGCTAGTACATCCAAGAGAGTACAAATGAACAACTCTGAGATATTGCATAATCCTGATAATGAAACTTGGTTAACTCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

931

Amino Acids

103.05

Weight (kDa)

7.99

Isoelectric Point (pI)

42.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANF_receptor	PF01094	44 - 390	2.9e-81	Receptor family ligand binding region
Peripla_BP_6	PF13458	84 - 317	6.8e-11	Periplasmic binding protein
Lig_chan-Glu_bd	PF10613	466 - 551	4.8e-09	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	506 - 787	3.7e-12	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	572 - 819	2e-38	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003979)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17260 AT4G35290 AT4G35290 AT4G35290 AT4G35290 AT4G35290 AT4G35290
fragaria_vesca	FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810
malus_domestica	MD05G1139700.v1.1 MD10G1141900.v1.1
prunus_persica	Prupe.8G180400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280931
rosa_laevigata	RLG00000013015
rosa_multiflora	Rmu_ssc0000288.1_g000001
rosa_roxburghii	Rroxscaffold_7G00187480
rosa_rugosa	Rorug06G0136800 Rorug06G0136900 Rorug06G0137000 Rorug06G0137000 Rorug06G0137000
rosa_samantha	Rh6AG249300 Rh6CG252000 Rh6DG243800
rosa_wichuraiana	Rw6G005260 Rw6G021570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2374
AbsI	CCTCGAGG	1 cut(s)	1314
Acc36I	ACCTGC	1 cut(s)	79
Acc65I	GGTACC	1 cut(s)	971
AccB1I	GGYRCC	2 cut(s)	971, 1654
AccI	GTMKAC	1 cut(s)	1000
AciI	CCGC	3 cut(s)	186, 1730, 2590
AclWI	GGATC	5 cut(s)	164, 341, 563, 969, 1759
AcoI	YGGCCR	3 cut(s)	1057, 1504, 2551
AcsI	RAATTY	4 cut(s)	194, 1556, 2234, 2340
AcuI	CTGAAG	3 cut(s)	1533, 2318, 2538
AcvI	CACGTG	1 cut(s)	319
AfaI	GTAC	9 cut(s)	371, 558, 973, 1362, 1408, 2501, 2575, 2719, 2733
AfiI	CCNNNNNNNGG	3 cut(s)	1010, 1337, 1997
AflII	CTTAAG	1 cut(s)	2141
AflIII	ACRYGT	2 cut(s)	316, 2292
AgeI	ACCGGT	1 cut(s)	1097
AhlI	ACTAGT	1 cut(s)	995
AjnI	CCWGG	1 cut(s)	1291
AloI	GAACNNNNNNTCC	2 cut(s)	2070, 2102
Alw26I	GTCTC	5 cut(s)	420, 633, 1106, 1140, 2265
AlwI	GGATC	5 cut(s)	164, 341, 563, 969, 1759
AlwNI	CAGNNNCTG	1 cut(s)	1069
Ama87I	CYCGRG	4 cut(s)	582, 630, 655, 1314
AoxI	GGCC	7 cut(s)	664, 1057, 1289, 1504, 1798, 2228, 2551
ApeKI	GCWGC	6 cut(s)	146, 1448, 1565, 1696, 2159, 2559
ApoI	RAATTY	4 cut(s)	194, 1556, 2234, 2340
AsiGI	ACCGGT	1 cut(s)	1097
Asp718I	GGTACC	1 cut(s)	971
AspA2I	CCTAGG	1 cut(s)	1805
AspS9I	GGNCC	5 cut(s)	263, 1109, 1799, 2177, 2228
AsuHPI	GGTGA	7 cut(s)	524, 1511, 1586, 1980, 2109, 2230, 2519
AsuNHI	GCTAGC	1 cut(s)	1949
AvaI	CYCGRG	4 cut(s)	582, 630, 655, 1314
AvaII	GGWCC	3 cut(s)	263, 1109, 2177
AvrII	CCTAGG	1 cut(s)	1805
AxyI	CCTNAGG	1 cut(s)	2504
BaeGI	GKGCMC	1 cut(s)	1233
BaeI	ACNNNNGTAYC	2 cut(s)	1398, 1431
BalI	TGGCCA	3 cut(s)	1059, 1506, 2553
BanI	GGYRCC	2 cut(s)	971, 1654
BanII	GRGCYC	1 cut(s)	2140
BbrPI	CACGTG	1 cut(s)	319
BbsI	GAAGAC	1 cut(s)	785
BbvCI	CCTCAGC	1 cut(s)	150
BbvI	GCAGC	6 cut(s)	158, 1460, 1552, 1708, 2146, 2571
BccI	CCATC	5 cut(s)	697, 800, 1046, 1252, 1496
BceAI	ACGGC	2 cut(s)	307, 1684
BciT130I	CCWGG	1 cut(s)	1293
BciVI	GTATCC	2 cut(s)	1190, 1418
BclI	TGATCA	3 cut(s)	400, 481, 2524
BcoDI	GTCTC	5 cut(s)	420, 633, 1106, 1140, 2265
BcuI	ACTAGT	1 cut(s)	995
BfmI	CTRYAG	3 cut(s)	223, 365, 2031
BfrI	CTTAAG	1 cut(s)	2141
BfuAI	ACCTGC	1 cut(s)	79
BfuI	GTATCC	2 cut(s)	1190, 1418
BglI	GCCNNNNNGGC	1 cut(s)	143
BisI	GCNGC	8 cut(s)	147, 1449, 1566, 1697, 1731, 2160, 2560, 2590
BlnI	CCTAGG	1 cut(s)	1805
BlsI	GCNGC	8 cut(s)	148, 1450, 1567, 1698, 1732, 2161, 2561, 2591
BmcAI	AGTACT	1 cut(s)	558
Bme1390I	CCNGG	1 cut(s)	1293
Bme18I	GGWCC	3 cut(s)	263, 1109, 2177
BmeT110I	CYCGRG	4 cut(s)	582, 630, 655, 1314
BmgT120I	GGNCC	5 cut(s)	263, 1109, 1799, 2177, 2228
BmiI	GGNNCC	4 cut(s)	265, 973, 1656, 2106
BmrFI	CCNGG	1 cut(s)	1293
BmsI	GCATC	5 cut(s)	71, 249, 1552, 2681, 2701
BmtI	GCTAGC	1 cut(s)	1953
BpiI	GAAGAC	1 cut(s)	785
BpmI	CTGGAG	2 cut(s)	1122, 1314
Bpu10I	CCTNAGC	1 cut(s)	150
BpuEI	CTTGAG	1 cut(s)	1660
BsaAI	YACGTR	1 cut(s)	319
BsaBI	GATNNNNATC	1 cut(s)	480
BsaI	GGTCTC	2 cut(s)	1106, 1140
BsaJI	CCNNGG	4 cut(s)	796, 1795, 1805, 2554
BsaWI	WCCGGW	2 cut(s)	826, 1097
BsaXI	ACNNNNNCTCC	2 cut(s)	417, 447
Bsc4I	CCNNNNNNNGG	3 cut(s)	1010, 1337, 1997
Bse118I	RCCGGY	1 cut(s)	1097
Bse1I	ACTGG	7 cut(s)	1111, 1119, 1139, 1205, 1256, 2002, 2106
Bse21I	CCTNAGG	1 cut(s)	2504
Bse3DI	GCAATG	2 cut(s)	1578, 2460
Bse8I	GATNNNNATC	1 cut(s)	480
BseBI	CCWGG	1 cut(s)	1293
BseDI	CCNNGG	4 cut(s)	796, 1795, 1805, 2554
BseGI	GGATG	6 cut(s)	163, 705, 792, 2085, 2716, 2720
BseJI	GATNNNNATC	1 cut(s)	480
BseLI	CCNNNNNNNGG	3 cut(s)	1010, 1337, 1997
BseMI	GCAATG	2 cut(s)	1578, 2460
BseMII	CTCAG	7 cut(s)	141, 250, 788, 2016, 2214, 2518, 2739
BseNI	ACTGG	7 cut(s)	1111, 1119, 1139, 1205, 1256, 2002, 2106
BseRI	GAGGAG	2 cut(s)	135, 167
BseSI	GKGCMC	1 cut(s)	1233
BseXI	GCAGC	6 cut(s)	158, 1460, 1552, 1708, 2146, 2571
BseYI	CCCAGC	1 cut(s)	2250
BsgI	GTGCAG	3 cut(s)	324, 375, 629
BshFI	GGCC	7 cut(s)	666, 1059, 1291, 1506, 1800, 2230, 2553
BshNI	GGYRCC	2 cut(s)	971, 1654
BshTI	ACCGGT	1 cut(s)	1097
BsiHKCI	CYCGRG	4 cut(s)	582, 630, 655, 1314
BsiSI	CCGG	2 cut(s)	827, 1098
BslFI	GGGAC	2 cut(s)	249, 2442
BslI	CCNNNNNNNGG	3 cut(s)	1010, 1337, 1997
BsmAI	GTCTC	5 cut(s)	420, 633, 1106, 1140, 2265
BsmFI	GGGAC	2 cut(s)	249, 2442
BsmI	GAATGC	3 cut(s)	169, 177, 421
BsnI	GGCC	7 cut(s)	666, 1059, 1291, 1506, 1800, 2230, 2553
Bso31I	GGTCTC	2 cut(s)	1106, 1140
BsoBI	CYCGRG	4 cut(s)	582, 630, 655, 1314
Bsp1286I	GDGCHC	2 cut(s)	1233, 2140
Bsp19I	CCATGG	1 cut(s)	2554
BspACI	CCGC	3 cut(s)	186, 1730, 2590
BspANI	GGCC	7 cut(s)	666, 1059, 1291, 1506, 1800, 2230, 2553
BspCNI	CTCAG	7 cut(s)	142, 249, 787, 2017, 2213, 2517, 2740
BspHI	TCATGA	2 cut(s)	211, 2344
BspLI	GGNNCC	4 cut(s)	265, 973, 1656, 2106
BspMAI	CTGCAG	1 cut(s)	369
BspMI	ACCTGC	1 cut(s)	79
BspOI	GCTAGC	1 cut(s)	1953
BspPI	GGATC	5 cut(s)	164, 341, 563, 969, 1759
BspT107I	GGYRCC	2 cut(s)	971, 1654
BspTI	CTTAAG	1 cut(s)	2141
BspTNI	GGTCTC	2 cut(s)	1106, 1140
BsrDI	GCAATG	2 cut(s)	1578, 2460
BsrFI	RCCGGY	1 cut(s)	1097
BsrI	ACTGG	7 cut(s)	1111, 1119, 1139, 1205, 1256, 2002, 2106
BssAI	RCCGGY	1 cut(s)	1097
BssECI	CCNNGG	4 cut(s)	796, 1795, 1805, 2554
BssNAI	GTATAC	1 cut(s)	1001
BssT1I	CCWWGG	3 cut(s)	796, 1805, 2554
Bst1107I	GTATAC	1 cut(s)	1001
Bst2UI	CCWGG	1 cut(s)	1293
Bst6I	CTCTTC	2 cut(s)	816, 2198
BstAFI	CTTAAG	1 cut(s)	2141
BstAPI	GCANNNNNTGC	1 cut(s)	1589
BstBAI	YACGTR	1 cut(s)	319
BstC8I	GCNNGC	4 cut(s)	365, 522, 1061, 1951
BstDEI	CTNAG	9 cut(s)	150, 182, 236, 774, 1661, 2025, 2200, 2504, 2748
BstDSI	CCRYGG	2 cut(s)	1795, 2554
BstF5I	GGATG	6 cut(s)	163, 705, 792, 2085, 2716, 2720
BstMAI	GTCTC	5 cut(s)	420, 633, 1106, 1140, 2265
BstMWI	GCNNNNNNNGC	5 cut(s)	98, 143, 183, 1589, 2559
BstNI	CCWGG	1 cut(s)	1293
BstNSI	RCATGY	3 cut(s)	2215, 2296, 2457
BstSCI	CCNGG	1 cut(s)	1291
BstSFI	CTRYAG	3 cut(s)	223, 365, 2031
BstSLI	GKGCMC	1 cut(s)	1233
BstV1I	GCAGC	6 cut(s)	158, 1460, 1552, 1708, 2146, 2571
BstV2I	GAAGAC	1 cut(s)	785
BstX2I	RGATCY	1 cut(s)	346
BstXI	CCANNNNNNTGG	3 cut(s)	751, 1251, 1716
BstYI	RGATCY	1 cut(s)	346
BstZ17I	GTATAC	1 cut(s)	1001
Bsu36I	CCTNAGG	1 cut(s)	2504
BsuI	GTATCC	2 cut(s)	1190, 1418
BsuRI	GGCC	7 cut(s)	666, 1059, 1291, 1506, 1800, 2230, 2553
BtgI	CCRYGG	2 cut(s)	1795, 2554
BtsCI	GGATG	6 cut(s)	163, 705, 792, 2085, 2716, 2720
BtsI	GCAGTG	3 cut(s)	336, 501, 2448
BveI	ACCTGC	1 cut(s)	79
Cac8I	GCNNGC	4 cut(s)	365, 522, 1061, 1951
CaiI	CAGNNNCTG	1 cut(s)	1069
CciI	TCATGA	2 cut(s)	211, 2344
Cfr10I	RCCGGY	1 cut(s)	1097
Cfr13I	GGNCC	5 cut(s)	263, 1109, 1799, 2177, 2228
CseI	GACGC	1 cut(s)	2364
Csp6I	GTAC	9 cut(s)	370, 557, 972, 1361, 1407, 2500, 2574, 2718, 2732
CspAI	ACCGGT	1 cut(s)	1097
CspCI	CAANNNNNGTGG	2 cut(s)	1590, 1625
CviAII	CATG	9 cut(s)	212, 280, 941, 1540, 2212, 2293, 2345, 2454, 2555
CviQI	GTAC	9 cut(s)	370, 557, 972, 1361, 1407, 2500, 2574, 2718, 2732
DdeI	CTNAG	9 cut(s)	150, 182, 236, 774, 1661, 2025, 2200, 2504, 2748
DrdI	GACNNNNNNGTC	1 cut(s)	2374
DseDI	GACNNNNNNGTC	1 cut(s)	2374
EaeI	YGGCCR	3 cut(s)	1057, 1504, 2551
Eam1104I	CTCTTC	2 cut(s)	816, 2198
EarI	CTCTTC	2 cut(s)	816, 2198
Eco130I	CCWWGG	3 cut(s)	796, 1805, 2554
Eco147I	AGGCCT	1 cut(s)	666
Eco24I	GRGCYC	1 cut(s)	2140
Eco31I	GGTCTC	2 cut(s)	1106, 1140
Eco32I	GATATC	1 cut(s)	1159
Eco47I	GGWCC	3 cut(s)	263, 1109, 2177
Eco57I	CTGAAG	3 cut(s)	1533, 2318, 2538
Eco72I	CACGTG	1 cut(s)	319
Eco81I	CCTNAGG	1 cut(s)	2504
Eco88I	CYCGRG	4 cut(s)	582, 630, 655, 1314
EcoO109I	RGGNCCY	1 cut(s)	2177
EcoRI	GAATTC	1 cut(s)	2234
EcoRII	CCWGG	1 cut(s)	1291
EcoRV	GATATC	1 cut(s)	1159
EcoT14I	CCWWGG	3 cut(s)	796, 1805, 2554
EcoT38I	GRGCYC	1 cut(s)	2140
ErhI	CCWWGG	3 cut(s)	796, 1805, 2554
FaeI	CATG	9 cut(s)	215, 283, 944, 1543, 2215, 2296, 2348, 2457, 2558
FaqI	GGGAC	2 cut(s)	249, 2442
FatI	CATG	9 cut(s)	211, 279, 940, 1539, 2211, 2292, 2344, 2453, 2554
FauNDI	CATATG	1 cut(s)	1153
FbaI	TGATCA	3 cut(s)	400, 481, 2524
FblI	GTMKAC	1 cut(s)	1000
Fnu4HI	GCNGC	8 cut(s)	147, 1449, 1566, 1697, 1731, 2160, 2560, 2590
FokI	GGATG	6 cut(s)	170, 712, 779, 2072, 2707, 2723
FriOI	GRGCYC	1 cut(s)	2140
Fsp4HI	GCNGC	8 cut(s)	147, 1449, 1566, 1697, 1731, 2160, 2560, 2590
GluI	GCNGC	8 cut(s)	147, 1449, 1566, 1697, 1731, 2160, 2560, 2590
GsaI	CCCAGC	1 cut(s)	2254
GsuI	CTGGAG	2 cut(s)	1122, 1314
HaeIII	GGCC	7 cut(s)	666, 1059, 1291, 1506, 1800, 2230, 2553
HapII	CCGG	2 cut(s)	827, 1098
HgaI	GACGC	1 cut(s)	2364
Hin1II	CATG	9 cut(s)	215, 283, 944, 1543, 2215, 2296, 2348, 2457, 2558
HincII	GTYRAC	3 cut(s)	1537, 2298, 2784
HindII	GTYRAC	3 cut(s)	1537, 2298, 2784
HindIII	AAGCTT	2 cut(s)	518, 2419
HpaI	GTTAAC	2 cut(s)	1537, 2784
HpaII	CCGG	2 cut(s)	827, 1098
HphI	GGTGA	7 cut(s)	524, 1511, 1586, 1980, 2109, 2230, 2519
Hpy166II	GTNNAC	9 cut(s)	82, 744, 818, 1001, 1112, 1537, 2117, 2298, 2784
Hpy8I	GTNNAC	9 cut(s)	82, 744, 818, 1001, 1112, 1537, 2117, 2298, 2784
Hpy99I	CGWCG	1 cut(s)	2396
HpyAV	CCTTC	5 cut(s)	50, 991, 1158, 2168, 2556
HpyCH4IV	ACGT	2 cut(s)	318, 2394
HpyF10VI	GCNNNNNNNGC	5 cut(s)	98, 143, 183, 1589, 2559
HpyF3I	CTNAG	9 cut(s)	150, 182, 236, 774, 1661, 2025, 2200, 2504, 2748
HpySE526I	ACGT	2 cut(s)	318, 2394
Hsp92II	CATG	9 cut(s)	215, 283, 944, 1543, 2215, 2296, 2348, 2457, 2558
KpnI	GGTACC	1 cut(s)	975
Ksp22I	TGATCA	3 cut(s)	400, 481, 2524
KspAI	GTTAAC	2 cut(s)	1537, 2784
LmnI	GCTCC	2 cut(s)	59, 89
Lsp1109I	GCAGC	6 cut(s)	158, 1460, 1552, 1708, 2146, 2571
LweI	GCATC	5 cut(s)	71, 249, 1552, 2681, 2701
MaeII	ACGT	2 cut(s)	318, 2394
MaeIII	GTNAC	6 cut(s)	324, 499, 1392, 1574, 1986, 2376
MflI	RGATCY	1 cut(s)	346
MhlI	GDGCHC	2 cut(s)	1233, 2140
MlsI	TGGCCA	3 cut(s)	1059, 1506, 2553
MluNI	TGGCCA	3 cut(s)	1059, 1506, 2553
MlyI	GAGTC	3 cut(s)	635, 1643, 2267
MmeI	TCCRAC	5 cut(s)	22, 428, 596, 1174, 1729
Mox20I	TGGCCA	3 cut(s)	1059, 1506, 2553
MscI	TGGCCA	3 cut(s)	1059, 1506, 2553
MslI	CAYNNNNRTG	4 cut(s)	248, 1275, 1714, 2291
Msp20I	TGGCCA	3 cut(s)	1059, 1506, 2553
MspA1I	CMGCKG	2 cut(s)	149, 1730
MspCI	CTTAAG	1 cut(s)	2141
MspI	CCGG	2 cut(s)	827, 1098
MspR9I	CCNGG	1 cut(s)	1293
Mva1269I	GAATGC	3 cut(s)	169, 177, 421
MvaI	CCWGG	1 cut(s)	1293
MwoI	GCNNNNNNNGC	5 cut(s)	98, 143, 183, 1589, 2559
NcoI	CCATGG	1 cut(s)	2554
NdeI	CATATG	1 cut(s)	1153
NheI	GCTAGC	1 cut(s)	1949
NlaIII	CATG	9 cut(s)	215, 283, 944, 1543, 2215, 2296, 2348, 2457, 2558
NlaIV	GGNNCC	4 cut(s)	265, 973, 1656, 2106
NmuCI	GTSAC	5 cut(s)	499, 1392, 1574, 1986, 2376
NspI	RCATGY	3 cut(s)	2215, 2296, 2457
PaeR7I	CTCGAG	4 cut(s)	582, 630, 655, 1314
PagI	TCATGA	2 cut(s)	211, 2344
PceI	AGGCCT	1 cut(s)	666
PciI	ACATGT	1 cut(s)	2292
PctI	GAATGC	3 cut(s)	169, 177, 421
PinAI	ACCGGT	1 cut(s)	1097
PkrI	GCNGC	8 cut(s)	148, 1450, 1567, 1698, 1732, 2161, 2561, 2591
PleI	GAGTC	3 cut(s)	634, 1642, 2267
PmaCI	CACGTG	1 cut(s)	319
PmlI	CACGTG	1 cut(s)	319
PpsI	GAGTC	3 cut(s)	634, 1642, 2267
Ppu21I	YACGTR	1 cut(s)	319
PpuMI	RGGWCCY	1 cut(s)	2177
PscI	ACATGT	1 cut(s)	2292
Psp5II	RGGWCCY	1 cut(s)	2177
Psp6I	CCWGG	1 cut(s)	1291
PspCI	CACGTG	1 cut(s)	319
PspFI	CCCAGC	1 cut(s)	2250
PspGI	CCWGG	1 cut(s)	1291
PspN4I	GGNNCC	4 cut(s)	265, 973, 1656, 2106
PspPI	GGNCC	5 cut(s)	263, 1109, 1799, 2177, 2228
PspPPI	RGGWCCY	1 cut(s)	2177
PspXI	VCTCGAGB	1 cut(s)	1314
PstI	CTGCAG	1 cut(s)	369
PstNI	CAGNNNCTG	1 cut(s)	1069
PsuI	RGATCY	1 cut(s)	346
PvuII	CAGCTG	1 cut(s)	149
RsaI	GTAC	9 cut(s)	371, 558, 973, 1362, 1408, 2501, 2575, 2719, 2733
RsaNI	GTAC	9 cut(s)	370, 557, 972, 1361, 1407, 2500, 2574, 2718, 2732
RseI	CAYNNNNRTG	4 cut(s)	248, 1275, 1714, 2291
SatI	GCNGC	8 cut(s)	147, 1449, 1566, 1697, 1731, 2160, 2560, 2590
Sau96I	GGNCC	5 cut(s)	263, 1109, 1799, 2177, 2228
ScaI	AGTACT	1 cut(s)	558
SchI	GAGTC	3 cut(s)	635, 1643, 2267
ScrFI	CCNGG	1 cut(s)	1293
SduI	GDGCHC	2 cut(s)	1233, 2140
SfaNI	GCATC	5 cut(s)	71, 249, 1552, 2681, 2701
SfcI	CTRYAG	3 cut(s)	223, 365, 2031
Sfr274I	CTCGAG	4 cut(s)	582, 630, 655, 1314
SinI	GGWCC	3 cut(s)	263, 1109, 2177
SlaI	CTCGAG	4 cut(s)	582, 630, 655, 1314
SmiMI	CAYNNNNRTG	4 cut(s)	248, 1275, 1714, 2291
SmlI	CTYRAG	6 cut(s)	582, 630, 655, 1314, 1675, 2141
SmoI	CTYRAG	6 cut(s)	582, 630, 655, 1314, 1675, 2141
SpeI	ACTAGT	1 cut(s)	995
SseBI	AGGCCT	1 cut(s)	666
SsiI	CCGC	3 cut(s)	186, 1730, 2590
StuI	AGGCCT	1 cut(s)	666
StyD4I	CCNGG	1 cut(s)	1291
StyI	CCWWGG	3 cut(s)	796, 1805, 2554
TaiI	ACGT	2 cut(s)	321, 2397
TaqI	TCGA	8 cut(s)	583, 631, 656, 1315, 1369, 1597, 2494, 2648
TatI	WGTACW	3 cut(s)	556, 2717, 2731
TauI	GCSGC	2 cut(s)	1733, 2592
TseFI	GTSAC	5 cut(s)	499, 1392, 1574, 1986, 2376
TseI	GCWGC	6 cut(s)	146, 1448, 1565, 1696, 2159, 2559
Tsp45I	GTSAC	5 cut(s)	499, 1392, 1574, 1986, 2376
TspGWI	ACGGA	2 cut(s)	1784, 2340
Vha464I	CTTAAG	1 cut(s)	2141
VpaK11BI	GGWCC	3 cut(s)	263, 1109, 2177
XapI	RAATTY	4 cut(s)	194, 1556, 2234, 2340
XbaI	TCTAGA	2 cut(s)	2096, 2539
XceI	RCATGY	3 cut(s)	2215, 2296, 2457
XhoI	CTCGAG	4 cut(s)	582, 630, 655, 1314
XmaJI	CCTAGG	1 cut(s)	1805
XmiI	GTMKAC	1 cut(s)	1000
ZrmI	AGTACT	1 cut(s)	558

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.