Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0003979

Physical Location & Seq

Genomic Coordinates Forward (+)

42966222 .. 42969765

Gene Structure 3544 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G021570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2826 bp

ATGAATCTAGTTTGCCTCTTGGCAATCCTCATTATCTGTATTGGAAGATCCACAGAAGGAGCATCAAGACCTGCTGTTGTGAACGTTGGAGCTATGTTTGCAGTAAGCACCATTAATGGAGGAGTCTCAAAGATTGCCATTAAGGCTGCCGAGGAGGATGTGAATGCCGATCCAAGCATTCTTAGTGGAACTAAATTTTCTGTATCTATGCATGATTCGAACTACAGTGGATTTCTCAGCATCATCGGAGCACTAAATTACATGGAGTCTGATACCGTGGCTATAATTGGTCCTCAAACTTCTGTAATGGCTCATATAGTCTCACATCTTGCAAATGAACTACACGTGCCGTTACTGTCATTCACTGCACTAGATCCCACCCTATCAAGCCTGCAGTACCCTTACTTTCTTCAAACTGCACCAAATGATCAATTCCAGATGAATGCTATTGGAGACATGGTTAGTTATTTCGGTTGGAAAGAGGTGGTTGCACTTTTCACTGATGATGATCAGAGCCGAAATGGTGTTACTGCATTAGGTGATAAGCTTGCAGAAAAAATGCACAAGATTTCTTACAAGGCAGTACTTCCACCTGATCCAACAGCAACTCGAGACCAAGTTAAGACTGAGCTGGTAAAGATTCAGATAATGGAGTCTCGAGTAATTGTTCTACACACATTCTCGAGAACCGGCCTCTTGGTTTTTGATGTTGCAAACGAACTTGGGATGATGGAGAGTGGATATGTTTGGATAGCTACTGCTTGGTTGTCCACAGTTTTGGATTCAACTTCCCCACTTCCTCAGAAGACTAAAGACTCCATCGAAGGAGCTCTCACTCTTCGTCCGCACACACCGGATTCAAAAAGGAAAAGAGCTTTCATTTCAAGGTGGAAGAAGTTGAGTAATGGCACTAGAGGATTGAACCCTTATGCTCTATATGCCTATGACACTGTTTGGATTATTGCTCATGCTGTCAATTTGCTTTTGGATCAGGGAGGTACCATTTCTTTTTCCAATCATGCTAGCATACCTGGTTATAGGGGAGGGATTATGAACCTTTCTGCATTAAGCATTTTTAATGGTGGCAAGCAGTTGCTGGAAAACATATTGCAAACCAACACGACCGGTCTCACTGGTCCACTGGCTTTTCATTCAGACAGGTCCCTAGTGAATCCTTCCTATGATATCATTAACATAATTGAAAATGGATATCAACAGATTGGATACTGGTCTAACAATTCAGGGCTATCTGTTGTGCCTCCTGAGACAGCACCCAACCGGTCAAGTTCAAACCAACATTTAGCTGCTGTTGTATGGCCTGGAGGGACTACAGATAAGCCCCGAGGGTGGGTTTTTCCAAACAATGGGAAGCAGTTAATAATTGGAATACCAGATAGAGTAAGCTATCGCGACTTTGTGTCACGACAAAATGGTACAGATATAGCTAAAGGATACTGCATTGACATTTTCCTTGCAGCCATAAAGTTGCTTCCCTATGCAGTTCCACATAGGTTTGAGCTGTTTGGTGATGGCCACAAGAACCCAAGCTACTACGAGCTTGTTAGCAATATCCCTTCAGGTAAGTTTGATGCTGCTGTTGGTGACATTGCAATAGTTGCAAACAGAACAAAGATTGTGGACTTCACTCAGCCATTTATAGAGTCAGGGCTAGTAGTGGTGGCACCAGTTAGGGGTTCAGATGCAAGAGCTTGGGCATTCTTGAAGCCGTTTTCTCCATTGATGTGGGGTGTCACAGCAGCTTTCTTCCTAATTGTTGGATCAGTCCTGTGGGTTCTTGAACATAGATTAAATGATGATTTCCGGGGCCCTCCTAGGAAACAGATTGGCACAGTTTTATGGTTTAGCTTCTCTACTATGTTTTTTGCTCATAGAGAAAATACTGTGAGCTTTCTAGGGCGAATGGTGCTAATCATCTGGCTTTTTATAATTCTGATAATCAACTCAAGCTACACAGCTAGCCTGACATCAATGCTCACAGTACAACAATTATCTTCACCCATCACTGGAATCGATACCTTGATATCTAGCTTCGATCCTATAGGATTCCAAGTAGGGTCTTTTGCTGAAAACTATTTGATCGAGGAACTCAACATCCCAAAATCTAGACTGGTTCCTCTTGGTTCACCAGAAGAATATGCAAATGCCCTCAAGGAAAGAACTGTTGCTGCTGTGGTTGATGAAAGGCCTTATATAGAACTCTTCCTCTCAGATAACTGCATGTTCTCAATCAGAGGGACAGAATTCACCAAAAGTGGCTGGGGATTTGCATTTCCAAGAGACTCTCCTTTAGCCATAGATATGTCAACTGCCATTCTCACTCTATCAGAGAACGGTGAGCTTCAGCAAATTCATGACAAATGGCTATCAAAGAAGGCTTGTGTTTCACAAACCGCTGACCTCGAATCCGACCAGCTTCAACTACAAAGCTTCTGGGGAGTATTCCTAATAGTTGCCACTGCATGTTTCATTGCTTTAGTCATTCACTTTTCGTTGGCTTTTAGACAATACCTCCGGCGTTCCCCTGAAGATGATCATCATTCTGATCTAGAGCCGTCTGGCCATGGCAGCACAAGTTCTGGTACTACTGCAACACGCCGCCTCACGTTCTTGTCATTCATTGACGAAAAGAAAGATCAATCAAAAGATAATAAGTCAAAAAGAAAACGTGAGGAAATCATGCGAAAGTCATCTAATTGGCATGAAAAGGAAGATGTATCAAGGAATGTTAGTACATCCAAGAGAGTGCAAGTGAACAAGTCTGAGATATTGCATACTTCTGATAATGATACTTGGTTAACTCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

941

Amino Acids

104.14

Weight (kDa)

6.44

Isoelectric Point (pI)

43.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	39 - 326	5.6e-15	Periplasmic binding protein
ANF_receptor	PF01094	44 - 400	5.7e-88	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	476 - 561	3.4e-08	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	519 - 796	6.4e-09	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	581 - 828	2.8e-36	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003979)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17260 AT4G35290 AT4G35290 AT4G35290 AT4G35290 AT4G35290 AT4G35290
fragaria_vesca	FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810
malus_domestica	MD05G1139700.v1.1 MD10G1141900.v1.1
prunus_persica	Prupe.8G180400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280931
rosa_laevigata	RLG00000013015
rosa_multiflora	Rmu_ssc0000288.1_g000001
rosa_roxburghii	Rroxscaffold_7G00187480
rosa_rugosa	Rorug06G0136800 Rorug06G0136900 Rorug06G0137000 Rorug06G0137000 Rorug06G0137000
rosa_samantha	Rh6AG249300 Rh6CG252000 Rh6DG243800
rosa_wichuraiana	Rw6G005260 Rw6G021570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1946
Acc36I	ACCTGC	1 cut(s)	79
Acc65I	GGTACC	1 cut(s)	998
AccB1I	GGYRCC	2 cut(s)	998, 1681
AccB7I	CCANNNNNTGG	1 cut(s)	1364
AccII	CGCG	1 cut(s)	1410
AciI	CCGC	3 cut(s)	845, 2412, 2617
AclI	AACGTT	1 cut(s)	84
AclWI	GGATC	7 cut(s)	42, 164, 368, 590, 996, 1786, 2048
AcoI	YGGCCR	2 cut(s)	1531, 2578
AcsI	RAATTY	3 cut(s)	194, 2261, 2367
AcuI	CTGAAG	3 cut(s)	1560, 2345, 2565
AcvI	CACGTG	1 cut(s)	346
AfaI	GTAC	7 cut(s)	398, 585, 1000, 1435, 2001, 2602, 2752
AfiI	CCNNNNNNNGG	5 cut(s)	1347, 1348, 1364, 1691, 2024
AflIII	ACRYGT	1 cut(s)	343
AgeI	ACCGGT	2 cut(s)	1124, 1278
AhdI	GACNNNNNGTC	1 cut(s)	1417
AjnI	CCWGG	2 cut(s)	1030, 1318
AloI	GAACNNNNNNTCC	2 cut(s)	2097, 2129
Alw21I	GWGCWC	2 cut(s)	253, 832
Alw26I	GTCTC	8 cut(s)	130, 325, 447, 606, 660, 1133, 1259, 2292
AlwI	GGATC	7 cut(s)	42, 164, 368, 590, 996, 1786, 2048
AlwNI	CAGNNNCTG	1 cut(s)	1096
Ama87I	CYCGRG	4 cut(s)	609, 657, 682, 1341
AoxI	GGCC	6 cut(s)	691, 1316, 1531, 1825, 2204, 2578
ApaI	GGGCCC	1 cut(s)	1829
ApeKI	GCWGC	7 cut(s)	146, 1304, 1475, 1592, 1757, 2186, 2586
ApoI	RAATTY	3 cut(s)	194, 2261, 2367
AseI	ATTAAT	1 cut(s)	114
AsiGI	ACCGGT	2 cut(s)	1124, 1278
Asp718I	GGTACC	1 cut(s)	998
AspA2I	CCTAGG	1 cut(s)	1832
AspS9I	GGNCC	5 cut(s)	290, 1136, 1161, 1825, 1826
AsuC2I	CCSGG	1 cut(s)	1823
AsuHPI	GGTGA	7 cut(s)	551, 1538, 1613, 2007, 2136, 2257, 2366
AsuII	TTCGAA	1 cut(s)	218
AsuNHI	GCTAGC	2 cut(s)	1022, 1976
AvaI	CYCGRG	4 cut(s)	609, 657, 682, 1341
AvaII	GGWCC	3 cut(s)	290, 1136, 1161
AvrII	CCTAGG	1 cut(s)	1832
BaeGI	GKGCMC	1 cut(s)	1829
BalI	TGGCCA	2 cut(s)	1533, 2580
BanI	GGYRCC	2 cut(s)	998, 1681
BanII	GRGCYC	2 cut(s)	832, 1829
BbrPI	CACGTG	1 cut(s)	346
BbsI	GAAGAC	1 cut(s)	812
Bbv12I	GWGCWC	2 cut(s)	253, 832
BbvI	GCAGC	7 cut(s)	133, 1291, 1487, 1579, 1769, 2173, 2598
BccI	CCATC	4 cut(s)	724, 827, 1523, 2027
BceAI	ACGGC	3 cut(s)	334, 1711, 2557
BciT130I	CCWGG	2 cut(s)	1032, 1320
BciVI	GTATCC	2 cut(s)	1217, 1445
BclI	TGATCA	3 cut(s)	427, 508, 2551
BcnI	CCSGG	1 cut(s)	1823
BcoDI	GTCTC	8 cut(s)	130, 325, 447, 606, 660, 1133, 1259, 2292
BfmI	CTRYAG	4 cut(s)	223, 392, 1329, 2058
BfuAI	ACCTGC	1 cut(s)	79
BfuI	GTATCC	2 cut(s)	1217, 1445
BglI	GCCNNNNNGGC	1 cut(s)	143
BisI	GCNGC	8 cut(s)	147, 1305, 1476, 1593, 1758, 2187, 2587, 2617
BlnI	CCTAGG	1 cut(s)	1832
BlsI	GCNGC	8 cut(s)	148, 1306, 1477, 1594, 1759, 2188, 2588, 2618
BmcAI	AGTACT	1 cut(s)	585
Bme1390I	CCNGG	3 cut(s)	1032, 1320, 1823
Bme18I	GGWCC	3 cut(s)	290, 1136, 1161
BmeRI	GACNNNNNGTC	1 cut(s)	1417
BmeT110I	CYCGRG	4 cut(s)	609, 657, 682, 1341
BmgT120I	GGNCC	5 cut(s)	290, 1136, 1161, 1825, 1826
BmiI	GGNNCC	6 cut(s)	1000, 1163, 1683, 1826, 1827, 2133
BmrFI	CCNGG	3 cut(s)	1032, 1320, 1823
BmsI	GCATC	4 cut(s)	71, 249, 1579, 1690
BmtI	GCTAGC	2 cut(s)	1026, 1980
BpiI	GAAGAC	1 cut(s)	812
BpmI	CTGGAG	1 cut(s)	1341
Bpu14I	TTCGAA	1 cut(s)	218
BpuEI	CTTGAG	2 cut(s)	1948, 2153
BpuMI	CCSGG	1 cut(s)	1823
Bsa29I	ATCGAT	1 cut(s)	2031
BsaAI	YACGTR	1 cut(s)	346
BsaBI	GATNNNNATC	2 cut(s)	507, 2553
BsaI	GGTCTC	2 cut(s)	606, 1133
BsaJI	CCNNGG	6 cut(s)	150, 276, 1342, 1822, 1832, 2581
BsaWI	WCCGGW	3 cut(s)	853, 1124, 1278
BsaXI	ACNNNNNCTCC	4 cut(s)	444, 474, 2514, 2544
Bsc4I	CCNNNNNNNGG	5 cut(s)	1347, 1348, 1364, 1691, 2024
Bse118I	RCCGGY	3 cut(s)	689, 1124, 1278
Bse1I	ACTGG	6 cut(s)	1138, 1146, 1232, 1685, 2029, 2133
Bse3DI	GCAATG	2 cut(s)	1605, 2487
Bse8I	GATNNNNATC	2 cut(s)	507, 2553
BseBI	CCWGG	2 cut(s)	1032, 1320
BseCI	ATCGAT	1 cut(s)	2031
BseDI	CCNNGG	6 cut(s)	150, 276, 1342, 1822, 1832, 2581
BseGI	GGATG	4 cut(s)	163, 732, 2112, 2753
BseJI	GATNNNNATC	2 cut(s)	507, 2553
BseLI	CCNNNNNNNGG	5 cut(s)	1347, 1348, 1364, 1691, 2024
BseMI	GCAATG	2 cut(s)	1605, 2487
BseMII	CTCAG	7 cut(s)	250, 618, 815, 1254, 1661, 2241, 2772
BseNI	ACTGG	6 cut(s)	1138, 1146, 1232, 1685, 2029, 2133
BseRI	GAGGAG	2 cut(s)	135, 167
BseSI	GKGCMC	1 cut(s)	1829
BseXI	GCAGC	7 cut(s)	133, 1291, 1487, 1579, 1769, 2173, 2598
BseYI	CCCAGC	1 cut(s)	2277
BsgI	GTGCAG	2 cut(s)	351, 402
Bsh1236I	CGCG	1 cut(s)	1410
Bsh1285I	CGRYCG	1 cut(s)	1125
BshFI	GGCC	6 cut(s)	693, 1318, 1533, 1827, 2206, 2580
BshNI	GGYRCC	2 cut(s)	998, 1681
BshTI	ACCGGT	2 cut(s)	1124, 1278
BshVI	ATCGAT	1 cut(s)	2031
BsiEI	CGRYCG	1 cut(s)	1125
BsiHKAI	GWGCWC	2 cut(s)	253, 832
BsiHKCI	CYCGRG	4 cut(s)	609, 657, 682, 1341
BsiSI	CCGG	6 cut(s)	690, 854, 1125, 1279, 1822, 2533
BslFI	GGGAC	3 cut(s)	1147, 1339, 2269
BslI	CCNNNNNNNGG	5 cut(s)	1347, 1348, 1364, 1691, 2024
BsmAI	GTCTC	8 cut(s)	130, 325, 447, 606, 660, 1133, 1259, 2292
BsmFI	GGGAC	3 cut(s)	1147, 1339, 2269
BsmI	GAATGC	4 cut(s)	169, 177, 448, 1715
BsnI	GGCC	6 cut(s)	693, 1318, 1533, 1827, 2206, 2580
Bso31I	GGTCTC	2 cut(s)	606, 1133
BsoBI	CYCGRG	4 cut(s)	609, 657, 682, 1341
Bsp119I	TTCGAA	1 cut(s)	218
Bsp120I	GGGCCC	1 cut(s)	1825
Bsp1286I	GDGCHC	3 cut(s)	253, 832, 1829
Bsp19I	CCATGG	1 cut(s)	2581
Bsp68I	TCGCGA	1 cut(s)	1410
BspACI	CCGC	3 cut(s)	845, 2412, 2617
BspANI	GGCC	6 cut(s)	693, 1318, 1533, 1827, 2206, 2580
BspCNI	CTCAG	7 cut(s)	249, 619, 814, 1255, 1660, 2240, 2773
BspDI	ATCGAT	1 cut(s)	2031
BspFNI	CGCG	1 cut(s)	1410
BspHI	TCATGA	1 cut(s)	2371
BspLI	GGNNCC	6 cut(s)	1000, 1163, 1683, 1826, 1827, 2133
BspMAI	CTGCAG	1 cut(s)	396
BspMI	ACCTGC	1 cut(s)	79
BspOI	GCTAGC	2 cut(s)	1026, 1980
BspPI	GGATC	7 cut(s)	42, 164, 368, 590, 996, 1786, 2048
BspT104I	TTCGAA	1 cut(s)	218
BspT107I	GGYRCC	2 cut(s)	998, 1681
BspTNI	GGTCTC	2 cut(s)	606, 1133
BsrDI	GCAATG	2 cut(s)	1605, 2487
BsrFI	RCCGGY	3 cut(s)	689, 1124, 1278
BsrI	ACTGG	6 cut(s)	1138, 1146, 1232, 1685, 2029, 2133
BssAI	RCCGGY	3 cut(s)	689, 1124, 1278
BssECI	CCNNGG	6 cut(s)	150, 276, 1342, 1822, 1832, 2581
BssT1I	CCWWGG	2 cut(s)	1832, 2581
Bst2UI	CCWGG	2 cut(s)	1032, 1320
Bst6I	CTCTTC	2 cut(s)	843, 2225
BstAPI	GCANNNNNTGC	1 cut(s)	1616
BstBAI	YACGTR	1 cut(s)	346
BstBI	TTCGAA	1 cut(s)	218
BstC8I	GCNNGC	5 cut(s)	392, 549, 1024, 1088, 1978
BstDEI	CTNAG	8 cut(s)	182, 236, 627, 801, 1263, 1647, 2227, 2781
BstDSI	CCRYGG	2 cut(s)	276, 2581
BstF5I	GGATG	4 cut(s)	163, 732, 2112, 2753
BstFNI	CGCG	1 cut(s)	1410
BstMAI	GTCTC	8 cut(s)	130, 325, 447, 606, 660, 1133, 1259, 2292
BstMCI	CGRYCG	1 cut(s)	1125
BstMWI	GCNNNNNNNGC	6 cut(s)	98, 143, 938, 1616, 1924, 2586
BstNI	CCWGG	2 cut(s)	1032, 1320
BstNSI	RCATGY	2 cut(s)	2242, 2484
BstSCI	CCNGG	3 cut(s)	1030, 1318, 1821
BstSFI	CTRYAG	4 cut(s)	223, 392, 1329, 2058
BstSLI	GKGCMC	1 cut(s)	1829
BstUI	CGCG	1 cut(s)	1410
BstV1I	GCAGC	7 cut(s)	133, 1291, 1487, 1579, 1769, 2173, 2598
BstV2I	GAAGAC	1 cut(s)	812
BstX2I	RGATCY	2 cut(s)	47, 373
BstXI	CCANNNNNNTGG	2 cut(s)	778, 1743
BstYI	RGATCY	2 cut(s)	47, 373
Bsu15I	ATCGAT	1 cut(s)	2031
BsuI	GTATCC	2 cut(s)	1217, 1445
BsuRI	GGCC	6 cut(s)	693, 1318, 1533, 1827, 2206, 2580
BsuTUI	ATCGAT	1 cut(s)	2031
BtgI	CCRYGG	2 cut(s)	276, 2581
BtsCI	GGATG	4 cut(s)	163, 732, 2112, 2753
BtsI	GCAGTG	2 cut(s)	363, 2475
BtsIMutI	CAGTG	8 cut(s)	232, 363, 498, 948, 1131, 1139, 2022, 2475
BtuMI	TCGCGA	1 cut(s)	1410
BveI	ACCTGC	1 cut(s)	79
Cac8I	GCNNGC	5 cut(s)	392, 549, 1024, 1088, 1978
CaiI	CAGNNNCTG	1 cut(s)	1096
CciI	TCATGA	1 cut(s)	2371
Cfr10I	RCCGGY	3 cut(s)	689, 1124, 1278
Cfr13I	GGNCC	5 cut(s)	290, 1136, 1161, 1825, 1826
ClaI	ATCGAT	1 cut(s)	2031
CsiI	ACCWGGT	1 cut(s)	1030
Csp6I	GTAC	7 cut(s)	397, 584, 999, 1434, 2000, 2601, 2751
CspAI	ACCGGT	2 cut(s)	1124, 1278
CspCI	CAANNNNNGTGG	2 cut(s)	1617, 1652
CviQI	GTAC	7 cut(s)	397, 584, 999, 1434, 2000, 2601, 2751
DdeI	CTNAG	8 cut(s)	182, 236, 627, 801, 1263, 1647, 2227, 2781
DriI	GACNNNNNGTC	1 cut(s)	1417
EaeI	YGGCCR	2 cut(s)	1531, 2578
Eam1104I	CTCTTC	2 cut(s)	843, 2225
Eam1105I	GACNNNNNGTC	1 cut(s)	1417
EarI	CTCTTC	2 cut(s)	843, 2225
Ecl136II	GAGCTC	1 cut(s)	830
Eco130I	CCWWGG	2 cut(s)	1832, 2581
Eco147I	AGGCCT	1 cut(s)	2206
Eco24I	GRGCYC	2 cut(s)	832, 1829
Eco31I	GGTCTC	2 cut(s)	606, 1133
Eco32I	GATATC	3 cut(s)	1186, 1211, 2043
Eco47I	GGWCC	3 cut(s)	290, 1136, 1161
Eco53kI	GAGCTC	1 cut(s)	830
Eco57I	CTGAAG	3 cut(s)	1560, 2345, 2565
Eco72I	CACGTG	1 cut(s)	346
Eco88I	CYCGRG	4 cut(s)	609, 657, 682, 1341
EcoICRI	GAGCTC	1 cut(s)	830
EcoO109I	RGGNCCY	3 cut(s)	1161, 1825, 1826
EcoRI	GAATTC	1 cut(s)	2261
EcoRII	CCWGG	2 cut(s)	1030, 1318
EcoRV	GATATC	3 cut(s)	1186, 1211, 2043
EcoT14I	CCWWGG	2 cut(s)	1832, 2581
EcoT22I	ATGCAT	1 cut(s)	213
EcoT38I	GRGCYC	2 cut(s)	832, 1829
ErhI	CCWWGG	2 cut(s)	1832, 2581
FaqI	GGGAC	3 cut(s)	1147, 1339, 2269
FbaI	TGATCA	3 cut(s)	427, 508, 2551
Fnu4HI	GCNGC	8 cut(s)	147, 1305, 1476, 1593, 1758, 2187, 2587, 2617
FokI	GGATG	4 cut(s)	170, 739, 2099, 2740
FriOI	GRGCYC	2 cut(s)	832, 1829
Fsp4HI	GCNGC	8 cut(s)	147, 1305, 1476, 1593, 1758, 2187, 2587, 2617
GluI	GCNGC	8 cut(s)	147, 1305, 1476, 1593, 1758, 2187, 2587, 2617
GsaI	CCCAGC	1 cut(s)	2281
GsuI	CTGGAG	1 cut(s)	1341
HaeIII	GGCC	6 cut(s)	693, 1318, 1533, 1827, 2206, 2580
HapII	CCGG	6 cut(s)	690, 854, 1125, 1279, 1822, 2533
HincII	GTYRAC	2 cut(s)	2325, 2817
HindII	GTYRAC	2 cut(s)	2325, 2817
HindIII	AAGCTT	2 cut(s)	545, 2446
HpaI	GTTAAC	1 cut(s)	2817
HpaII	CCGG	6 cut(s)	690, 854, 1125, 1279, 1822, 2533
HphI	GGTGA	7 cut(s)	551, 1538, 1613, 2007, 2136, 2257, 2366
Hpy166II	GTNNAC	8 cut(s)	82, 771, 1139, 1639, 2144, 2325, 2773, 2817
Hpy8I	GTNNAC	8 cut(s)	82, 771, 1139, 1639, 2144, 2325, 2773, 2817
HpyAV	CCTTC	5 cut(s)	50, 818, 1185, 1584, 2386
HpyCH4IV	ACGT	4 cut(s)	84, 345, 2624, 2686
HpyF10VI	GCNNNNNNNGC	6 cut(s)	98, 143, 938, 1616, 1924, 2586
HpyF3I	CTNAG	8 cut(s)	182, 236, 627, 801, 1263, 1647, 2227, 2781
HpySE526I	ACGT	4 cut(s)	84, 345, 2624, 2686
KpnI	GGTACC	1 cut(s)	1002
Ksp22I	TGATCA	3 cut(s)	427, 508, 2551
KspAI	GTTAAC	1 cut(s)	2817
LmnI	GCTCC	4 cut(s)	59, 89, 248, 827
Lsp1109I	GCAGC	7 cut(s)	133, 1291, 1487, 1579, 1769, 2173, 2598
LweI	GCATC	4 cut(s)	71, 249, 1579, 1690
MabI	ACCWGGT	1 cut(s)	1030
MaeII	ACGT	4 cut(s)	84, 345, 2624, 2686
MaeIII	GTNAC	5 cut(s)	351, 526, 1419, 1601, 1750
MflI	RGATCY	2 cut(s)	47, 373
MhlI	GDGCHC	3 cut(s)	253, 832, 1829
MlsI	TGGCCA	2 cut(s)	1533, 2580
MluNI	TGGCCA	2 cut(s)	1533, 2580
MlyI	GAGTC	6 cut(s)	132, 275, 662, 809, 1670, 2294
MmeI	TCCRAC	5 cut(s)	67, 455, 623, 1756, 2451
Mox20I	TGGCCA	2 cut(s)	1533, 2580
Mph1103I	ATGCAT	1 cut(s)	213
MscI	TGGCCA	2 cut(s)	1533, 2580
MslI	CAYNNNNRTG	2 cut(s)	1741, 2318
Msp20I	TGGCCA	2 cut(s)	1533, 2580
MspA1I	CMGCKG	1 cut(s)	2414
MspI	CCGG	6 cut(s)	690, 854, 1125, 1279, 1822, 2533
MspR9I	CCNGG	3 cut(s)	1032, 1320, 1823
Mva1269I	GAATGC	4 cut(s)	169, 177, 448, 1715
MvaI	CCWGG	2 cut(s)	1032, 1320
MvnI	CGCG	1 cut(s)	1410
MwoI	GCNNNNNNNGC	6 cut(s)	98, 143, 938, 1616, 1924, 2586
NciI	CCSGG	1 cut(s)	1823
NcoI	CCATGG	1 cut(s)	2581
NheI	GCTAGC	2 cut(s)	1022, 1976
NlaIV	GGNNCC	6 cut(s)	1000, 1163, 1683, 1826, 1827, 2133
NmeAIII	GCCGAG	1 cut(s)	175
NmuCI	GTSAC	3 cut(s)	1419, 1601, 1750
NruI	TCGCGA	1 cut(s)	1410
NsiI	ATGCAT	1 cut(s)	213
NspI	RCATGY	2 cut(s)	2242, 2484
NspV	TTCGAA	1 cut(s)	218
PaeR7I	CTCGAG	3 cut(s)	609, 657, 682
PagI	TCATGA	1 cut(s)	2371
PceI	AGGCCT	1 cut(s)	2206
PctI	GAATGC	4 cut(s)	169, 177, 448, 1715
PfeI	GAWTC	9 cut(s)	4, 215, 640, 782, 857, 1171, 2028, 2064, 2423
PflMI	CCANNNNNTGG	1 cut(s)	1364
PinAI	ACCGGT	2 cut(s)	1124, 1278
PkrI	GCNGC	8 cut(s)	148, 1306, 1477, 1594, 1759, 2188, 2588, 2618
PleI	GAGTC	6 cut(s)	131, 274, 661, 809, 1669, 2294
PmaCI	CACGTG	1 cut(s)	346
PmlI	CACGTG	1 cut(s)	346
PpsI	GAGTC	6 cut(s)	131, 274, 661, 809, 1669, 2294
Ppu21I	YACGTR	1 cut(s)	346
PpuMI	RGGWCCY	1 cut(s)	1161
PshBI	ATTAAT	1 cut(s)	114
PsiI	TTATAA	1 cut(s)	1946
Psp124BI	GAGCTC	1 cut(s)	832
Psp1406I	AACGTT	1 cut(s)	84
Psp5II	RGGWCCY	1 cut(s)	1161
Psp6I	CCWGG	2 cut(s)	1030, 1318
PspCI	CACGTG	1 cut(s)	346
PspFI	CCCAGC	1 cut(s)	2277
PspGI	CCWGG	2 cut(s)	1030, 1318
PspN4I	GGNNCC	6 cut(s)	1000, 1163, 1683, 1826, 1827, 2133
PspOMI	GGGCCC	1 cut(s)	1825
PspPI	GGNCC	5 cut(s)	290, 1136, 1161, 1825, 1826
PspPPI	RGGWCCY	1 cut(s)	1161
PsrI	GAACNNNNNNTAC	2 cut(s)	1532, 1564
PstI	CTGCAG	1 cut(s)	396
PstNI	CAGNNNCTG	1 cut(s)	1096
PsuI	RGATCY	2 cut(s)	47, 373
RruI	TCGCGA	1 cut(s)	1410
RsaI	GTAC	7 cut(s)	398, 585, 1000, 1435, 2001, 2602, 2752
RsaNI	GTAC	7 cut(s)	397, 584, 999, 1434, 2000, 2601, 2751
RseI	CAYNNNNRTG	2 cut(s)	1741, 2318
SacI	GAGCTC	1 cut(s)	832
SatI	GCNGC	8 cut(s)	147, 1305, 1476, 1593, 1758, 2187, 2587, 2617
Sau96I	GGNCC	5 cut(s)	290, 1136, 1161, 1825, 1826
ScaI	AGTACT	1 cut(s)	585
SchI	GAGTC	6 cut(s)	132, 275, 662, 809, 1670, 2294
ScrFI	CCNGG	3 cut(s)	1032, 1320, 1823
SduI	GDGCHC	3 cut(s)	253, 832, 1829
SexAI	ACCWGGT	1 cut(s)	1030
SfaNI	GCATC	4 cut(s)	71, 249, 1579, 1690
SfcI	CTRYAG	4 cut(s)	223, 392, 1329, 2058
Sfr274I	CTCGAG	3 cut(s)	609, 657, 682
SfuI	TTCGAA	1 cut(s)	218
SinI	GGWCC	3 cut(s)	290, 1136, 1161
SlaI	CTCGAG	3 cut(s)	609, 657, 682
SmiMI	CAYNNNNRTG	2 cut(s)	1741, 2318
SmlI	CTYRAG	5 cut(s)	609, 657, 682, 1963, 2168
SmoI	CTYRAG	5 cut(s)	609, 657, 682, 1963, 2168
SseBI	AGGCCT	1 cut(s)	2206
SsiI	CCGC	3 cut(s)	845, 2412, 2617
SstI	GAGCTC	1 cut(s)	832
StuI	AGGCCT	1 cut(s)	2206
StyD4I	CCNGG	3 cut(s)	1030, 1318, 1821
StyI	CCWWGG	2 cut(s)	1832, 2581
TaiI	ACGT	4 cut(s)	87, 348, 2627, 2689
TaqI	TCGA	9 cut(s)	218, 610, 658, 683, 822, 2031, 2052, 2100, 2421
TatI	WGTACW	3 cut(s)	583, 1999, 2750
TauI	GCSGC	1 cut(s)	2619
TfiI	GAWTC	9 cut(s)	4, 215, 640, 782, 857, 1171, 2028, 2064, 2423
TscAI	CASTG	8 cut(s)	232, 370, 505, 955, 1138, 1146, 2029, 2482
TseFI	GTSAC	3 cut(s)	1419, 1601, 1750
TseI	GCWGC	7 cut(s)	146, 1304, 1475, 1592, 1757, 2186, 2586
Tsp45I	GTSAC	3 cut(s)	1419, 1601, 1750
TspRI	CASTG	8 cut(s)	232, 370, 505, 955, 1138, 1146, 2029, 2482
Van91I	CCANNNNNTGG	1 cut(s)	1364
VpaK11BI	GGWCC	3 cut(s)	290, 1136, 1161
VspI	ATTAAT	1 cut(s)	114
XapI	RAATTY	3 cut(s)	194, 2261, 2367
XbaI	TCTAGA	2 cut(s)	2123, 2566
XceI	RCATGY	2 cut(s)	2242, 2484
XhoI	CTCGAG	3 cut(s)	609, 657, 682
XmaJI	CCTAGG	1 cut(s)	1832
ZrmI	AGTACT	1 cut(s)	585
Zsp2I	ATGCAT	1 cut(s)	213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.