Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0003979

Physical Location & Seq

Genomic Coordinates Reverse (-)

24887889 .. 24891430

Gene Structure 3542 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000013015

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2688 bp

ATGAATCTAGTTTGCCTCTTGGCAATCCTCATTATCTGTATTGGAAGATCCACAGAAGGAGCATCAAGACCTGCTGTTGTGAACATTGGAGCTATGTTTGCAGTAAGCACCATTAATGGAGGAGTCTCAAAGATTGCCATTAAGGCTGCCGAGGAGGATGTGAATGCCGATCCAAGCATTCTTAGTGGAACTAAATTTTCTGTATCTATTCATGATTCGAACTACAGTGGATTTCTCAGCATCATCGGAGCACTAAATTACATGGAGTCTGATACCGTGGCTATAGTTGGTCCTCAAACTTCTGTAATGGCTCATATAATCTCACATCTTGCAAATGAACTACACGTGCCGTTACTGTCATTCACTGCACTAGATCCCACCCTATCAAGCCTGCAGTACCCTTACTTTCTTCAAACTGCACCAAATGATCAATTCCAGATGAATGCTATTGGAGACATGGTTAGTTATTTCGGTTGGAAAGAGGTGGTTGCACTTTTCACTGATGATGATCAAAGCCGAAATGGTGTTACTGCATTAGGTGATAAGCTTGCAGAAAAAATGCACAAGATTTCTTACAAGGCAGTACTTCCACCTGATCCAACAGCAACTCGAGACCAAGTTAAGAATGAGCTGGTAAAGATTCAGATAATGGAGTCTCGAGTAATTGTTCTACACACATTCTCGAGAACCGGCCTCTTGGTTTTTGATGTTGCGAAGGAACTTGGGATGATGGAGAGTGGATATGTTTGGATAGCTACTGCTTGGTTGTCCACAGTTTTGGATTCAACTTCCCCACTTCCTCAGAAGACTAAAGACTCCATCGAAGGAGCTCTCACTCTTCGTCCGCACACACCGGATTCAAAAAGGAAAAGAGCTTTCATTTCAAGGTGGAAGAAGTTGAGTAATGGCACTAGAGGGTTGAACCCTTATGCTCTATATGCCTATGACACTGTTTGGATTATTGCTCATGCTGTCAACTTGCTTTTGGATCAGGGAGGTACCATTTCTTTTTCCAATCATGCTAGTATACCTCGTTATGGGGGAGGGATTATGAACCTTTCTGCATTAAGCATTTTTAATGGTGGCAAGCAGTTGCTGGAAAACATATTGCAAACCAACATGACCGGTCTCACTGGTCCACTGGCTTTTCATTCAGACAGGTCTCTAGTGAATCCTTCCTATGATATCATTAACATAATTGAAAATGGAGATCAACAGATTGGATACTGGTCTAACAATTCAGGGCTATCTGTTGTGCCCCCTGAGACAGCACCCAACCGGTCTAGTTCAAACCAACATTTAGCTGCTGTTGTATGGCCTGGAGGGACAACAGATAAGCCCCGAGGGTGGGTTTTTCCAAACAATGGGAAGCAATTAATAATTGGAGTGCCAGATAGAGTAAGCTATCGCGACTTTGTGTCACGACAAAATGGTACAGATATAGCTAAAGGATACTGCATTGACATTTTCCTTGCAGCCATAAAGTTGCTTCCCTATGCAGTTCCACATAAGTTTGAGCTGTTTGGTGATGGCCGCAAGAACCCAAGCTACTCCGAGCTTGTTAGCAATATCGCTTCAGGTGTAATAGTACTTCTGTACATTGACCATTCCAATACAGACTCTGCATATATACTAATCTGTAGATTCTATGATTCATCTGCCTTCCTTCTTTTATTTTTCAGGTTTAGCTTCTCTACTTTGTTTTTTGCTCATAGTAAGTCTTCTAATCTGGCATTGAATGTTGTGCTCCAACGAGAAAATACTGTGAGCTTACTAGGGCGAATGGTGCTAATCATCTGGCTTTTTATAATTCTGATAATCAACTCCAGCTACACAGCTAGCCTGACATCAATGCTCACAGTACAACAATTATCTTCACCCATCACTGGAATCGATACCTTGATATCTAGCTTCGAGCCTATAGGATTCCAAGTAGGGTCTTTTGCTGAAAACTATTTGATCGAGGAACTCAACATCCCAAAATCTAGACTGATTCCTCTTGGTTCACCAGAAGAATATGCAAATGCCCTCAAGGAAAGAACTGTTGCTGCTGTGGTTGATGAAAGGCCTTATATAGAACTCTTCCTCTCAGATAACTGCATGTTCTCAATCAGAGGGCCAGAATTCACCAAAAGTGGCTGGGGATTTGCATTTCCAAGAGACTCTCCTTTAGCCATAGATATGTCAACTGCCATTCTCACTCTATCAGAGAACGGTGAGCTTCAGAAAATGCATGACAAATGGTTATCAAGGAAGGCTTGTGCTTCACAAACCTCTGACATCGAATCTGACCAGCTTCAACTACAAAGCTTCTGGGGACTATTCCTAATAGTTGGCACTGCATGTTTCATTGCTCTAGTCATTCACTTTTCGTTGGCTTTTAGACAGTACCTCCGGCGTTCCCCTGAAGATGATCATCATTCAGACCTAGAGCCGTCTGGCCATGGCAGCACAAGTTCTGGTACTACTGCAACACGCCGCCTCACGTTCTTGTCATTCATTGACAAAAAGAAAGATCAATCAAAAGATAACAAGTCAAAAAGAAAACGTGAGGAAATCATGCGAAAGTCATCTAATTGGCATGAAAAGGAAGATGTATCAAGGAATGTTAGTACATCCAAGAGAGTACAAGTGAACAAGTCTGAGATATTGCATACTTCTGGTAATGATACTTGGTTAACTCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

896

Amino Acids

99.04

Weight (kDa)

6.74

Isoelectric Point (pI)

47.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	38 - 326	8.4e-16	Periplasmic binding protein
ANF_receptor	PF01094	44 - 400	1.3e-88	Receptor family ligand binding region
Lig_chan	PF00060	572 - 782	2.2e-21	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003979)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17260 AT4G35290 AT4G35290 AT4G35290 AT4G35290 AT4G35290 AT4G35290
fragaria_vesca	FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810
malus_domestica	MD05G1139700.v1.1 MD10G1141900.v1.1
prunus_persica	Prupe.8G180400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280931
rosa_laevigata	RLG00000013015
rosa_multiflora	Rmu_ssc0000288.1_g000001
rosa_roxburghii	Rroxscaffold_7G00187480
rosa_rugosa	Rorug06G0136800 Rorug06G0136900 Rorug06G0137000 Rorug06G0137000 Rorug06G0137000
rosa_samantha	Rh6AG249300 Rh6CG252000 Rh6DG243800
rosa_wichuraiana	Rw6G005260 Rw6G021570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1808
Acc36I	ACCTGC	1 cut(s)	79
Acc65I	GGTACC	1 cut(s)	998
AccB1I	GGYRCC	1 cut(s)	998
AccB7I	CCANNNNNTGG	1 cut(s)	1364
AccI	GTMKAC	1 cut(s)	1027
AccII	CGCG	1 cut(s)	1410
AciI	CCGC	3 cut(s)	845, 1534, 2479
AclWI	GGATC	5 cut(s)	42, 164, 368, 590, 996
AcoI	YGGCCR	2 cut(s)	1531, 2440
AcsI	RAATTY	2 cut(s)	194, 2123
AcuI	CTGAAG	3 cut(s)	1560, 2207, 2427
AcvI	CACGTG	1 cut(s)	346
AfiI	CCNNNNNNNGG	5 cut(s)	1037, 1347, 1348, 1364, 1886
AflIII	ACRYGT	1 cut(s)	343
AgeI	ACCGGT	2 cut(s)	1124, 1278
AgsI	TTSAA	9 cut(s)	413, 786, 861, 885, 922, 1202, 1290, 1738, 2300
AhdI	GACNNNNNGTC	1 cut(s)	1417
AjnI	CCWGG	1 cut(s)	1318
AloI	GAACNNNNNNTCC	2 cut(s)	1959, 1991
Alw21I	GWGCWC	3 cut(s)	253, 832, 1749
Alw26I	GTCTC	8 cut(s)	130, 447, 606, 660, 1133, 1167, 1259, 2154
AlwI	GGATC	5 cut(s)	42, 164, 368, 590, 996
AlwNI	CAGNNNCTG	2 cut(s)	1096, 1622
Ama87I	CYCGRG	4 cut(s)	609, 657, 682, 1341
AoxI	GGCC	6 cut(s)	691, 1316, 1531, 2066, 2117, 2440
ApeKI	GCWGC	5 cut(s)	146, 1304, 1475, 2048, 2448
ApoI	RAATTY	2 cut(s)	194, 2123
AseI	ATTAAT	2 cut(s)	114, 1376
AsiGI	ACCGGT	2 cut(s)	1124, 1278
Asp718I	GGTACC	1 cut(s)	998
AspS9I	GGNCC	3 cut(s)	290, 1136, 2117
AsuHPI	GGTGA	6 cut(s)	551, 1538, 1869, 1998, 2119, 2228
AsuII	TTCGAA	1 cut(s)	218
AsuNHI	GCTAGC	1 cut(s)	1838
AvaI	CYCGRG	4 cut(s)	609, 657, 682, 1341
AvaII	GGWCC	2 cut(s)	290, 1136
BaeGI	GKGCMC	1 cut(s)	1260
BalI	TGGCCA	1 cut(s)	2442
BanI	GGYRCC	1 cut(s)	998
BanII	GRGCYC	1 cut(s)	832
BarI	GAAGNNNNNNTAC	2 cut(s)	1575, 1607
BbrPI	CACGTG	1 cut(s)	346
BbsI	GAAGAC	2 cut(s)	812, 1713
Bbv12I	GWGCWC	3 cut(s)	253, 832, 1749
BbvI	GCAGC	5 cut(s)	133, 1291, 1487, 2035, 2460
BccI	CCATC	4 cut(s)	724, 827, 1523, 1889
BceAI	ACGGC	2 cut(s)	334, 2419
BciT130I	CCWGG	1 cut(s)	1320
BciVI	GTATCC	2 cut(s)	1217, 1445
BclI	TGATCA	3 cut(s)	427, 508, 2413
BcoDI	GTCTC	8 cut(s)	130, 447, 606, 660, 1133, 1167, 1259, 2154
BfmI	CTRYAG	5 cut(s)	223, 282, 392, 1639, 1920
BfuAI	ACCTGC	1 cut(s)	79
BfuI	GTATCC	2 cut(s)	1217, 1445
BglI	GCCNNNNNGGC	1 cut(s)	143
BisI	GCNGC	7 cut(s)	147, 1305, 1476, 1534, 2049, 2449, 2479
BlsI	GCNGC	7 cut(s)	148, 1306, 1477, 1535, 2050, 2450, 2480
BmcAI	AGTACT	2 cut(s)	585, 1590
Bme1390I	CCNGG	1 cut(s)	1320
Bme18I	GGWCC	2 cut(s)	290, 1136
BmeRI	GACNNNNNGTC	1 cut(s)	1417
BmeT110I	CYCGRG	4 cut(s)	609, 657, 682, 1341
BmgT120I	GGNCC	3 cut(s)	290, 1136, 2117
BmiI	GGNNCC	1 cut(s)	1000
BmrFI	CCNGG	1 cut(s)	1320
BmsI	GCATC	2 cut(s)	71, 249
BmtI	GCTAGC	1 cut(s)	1842
BpiI	GAAGAC	2 cut(s)	812, 1713
BpmI	CTGGAG	2 cut(s)	1341, 1810
Bpu14I	TTCGAA	1 cut(s)	218
BpuEI	CTTGAG	1 cut(s)	2015
Bsa29I	ATCGAT	1 cut(s)	1893
BsaAI	YACGTR	1 cut(s)	346
BsaBI	GATNNNNATC	2 cut(s)	507, 2415
BsaI	GGTCTC	3 cut(s)	606, 1133, 1167
BsaJI	CCNNGG	4 cut(s)	150, 276, 1342, 2443
BsaWI	WCCGGW	3 cut(s)	853, 1124, 1278
BsaXI	ACNNNNNCTCC	4 cut(s)	444, 474, 2376, 2406
Bsc4I	CCNNNNNNNGG	5 cut(s)	1037, 1347, 1348, 1364, 1886
Bse118I	RCCGGY	3 cut(s)	689, 1124, 1278
Bse1I	ACTGG	4 cut(s)	1138, 1146, 1232, 1891
Bse3DI	GCAATG	1 cut(s)	2349
Bse8I	GATNNNNATC	2 cut(s)	507, 2415
BseBI	CCWGG	1 cut(s)	1320
BseCI	ATCGAT	1 cut(s)	1893
BseDI	CCNNGG	4 cut(s)	150, 276, 1342, 2443
BseGI	GGATG	4 cut(s)	163, 732, 1974, 2615
BseJI	GATNNNNATC	2 cut(s)	507, 2415
BseLI	CCNNNNNNNGG	5 cut(s)	1037, 1347, 1348, 1364, 1886
BseMI	GCAATG	1 cut(s)	2349
BseMII	CTCAG	5 cut(s)	250, 815, 1254, 2103, 2634
BseNI	ACTGG	4 cut(s)	1138, 1146, 1232, 1891
BseRI	GAGGAG	2 cut(s)	135, 167
BseSI	GKGCMC	1 cut(s)	1260
BseXI	GCAGC	5 cut(s)	133, 1291, 1487, 2035, 2460
BseYI	CCCAGC	1 cut(s)	2139
BsgI	GTGCAG	2 cut(s)	351, 402
Bsh1236I	CGCG	1 cut(s)	1410
BshFI	GGCC	6 cut(s)	693, 1318, 1533, 2068, 2119, 2442
BshNI	GGYRCC	1 cut(s)	998
BshTI	ACCGGT	2 cut(s)	1124, 1278
BshVI	ATCGAT	1 cut(s)	1893
BsiHKAI	GWGCWC	3 cut(s)	253, 832, 1749
BsiHKCI	CYCGRG	4 cut(s)	609, 657, 682, 1341
BsiSI	CCGG	5 cut(s)	690, 854, 1125, 1279, 2395
BslFI	GGGAC	2 cut(s)	1339, 2331
BslI	CCNNNNNNNGG	5 cut(s)	1037, 1347, 1348, 1364, 1886
BsmAI	GTCTC	8 cut(s)	130, 447, 606, 660, 1133, 1167, 1259, 2154
BsmFI	GGGAC	2 cut(s)	1339, 2331
BsmI	GAATGC	3 cut(s)	169, 177, 448
BsnI	GGCC	6 cut(s)	693, 1318, 1533, 2068, 2119, 2442
Bso31I	GGTCTC	3 cut(s)	606, 1133, 1167
BsoBI	CYCGRG	4 cut(s)	609, 657, 682, 1341
Bsp119I	TTCGAA	1 cut(s)	218
Bsp1286I	GDGCHC	4 cut(s)	253, 832, 1260, 1749
Bsp1407I	TGTACA	1 cut(s)	1596
Bsp19I	CCATGG	1 cut(s)	2443
Bsp68I	TCGCGA	1 cut(s)	1410
BspACI	CCGC	3 cut(s)	845, 1534, 2479
BspANI	GGCC	6 cut(s)	693, 1318, 1533, 2068, 2119, 2442
BspCNI	CTCAG	5 cut(s)	249, 814, 1255, 2102, 2635
BspDI	ATCGAT	1 cut(s)	1893
BspFNI	CGCG	1 cut(s)	1410
BspHI	TCATGA	1 cut(s)	211
BspLI	GGNNCC	1 cut(s)	1000
BspMAI	CTGCAG	1 cut(s)	396
BspMI	ACCTGC	1 cut(s)	79
BspOI	GCTAGC	1 cut(s)	1842
BspPI	GGATC	5 cut(s)	42, 164, 368, 590, 996
BspT104I	TTCGAA	1 cut(s)	218
BspT107I	GGYRCC	1 cut(s)	998
BspTNI	GGTCTC	3 cut(s)	606, 1133, 1167
BsrDI	GCAATG	1 cut(s)	2349
BsrFI	RCCGGY	3 cut(s)	689, 1124, 1278
BsrGI	TGTACA	1 cut(s)	1596
BsrI	ACTGG	4 cut(s)	1138, 1146, 1232, 1891
BssAI	RCCGGY	3 cut(s)	689, 1124, 1278
BssECI	CCNNGG	4 cut(s)	150, 276, 1342, 2443
BssNAI	GTATAC	1 cut(s)	1028
BssT1I	CCWWGG	1 cut(s)	2443
Bst1107I	GTATAC	1 cut(s)	1028
Bst2UI	CCWGG	1 cut(s)	1320
Bst6I	CTCTTC	2 cut(s)	843, 2087
BstAUI	TGTACA	1 cut(s)	1596
BstBAI	YACGTR	1 cut(s)	346
BstBI	TTCGAA	1 cut(s)	218
BstC8I	GCNNGC	4 cut(s)	392, 549, 1088, 1840
BstDEI	CTNAG	6 cut(s)	182, 236, 801, 1263, 2089, 2643
BstDSI	CCRYGG	2 cut(s)	276, 2443
BstF5I	GGATG	4 cut(s)	163, 732, 1974, 2615
BstFNI	CGCG	1 cut(s)	1410
BstMAI	GTCTC	8 cut(s)	130, 447, 606, 660, 1133, 1167, 1259, 2154
BstMWI	GCNNNNNNNGC	5 cut(s)	98, 143, 938, 1786, 2448
BstNI	CCWGG	1 cut(s)	1320
BstNSI	RCATGY	2 cut(s)	2104, 2346
BstSCI	CCNGG	1 cut(s)	1318
BstSFI	CTRYAG	5 cut(s)	223, 282, 392, 1639, 1920
BstSLI	GKGCMC	1 cut(s)	1260
BstUI	CGCG	1 cut(s)	1410
BstV1I	GCAGC	5 cut(s)	133, 1291, 1487, 2035, 2460
BstV2I	GAAGAC	2 cut(s)	812, 1713
BstX2I	RGATCY	2 cut(s)	47, 373
BstXI	CCANNNNNNTGG	1 cut(s)	778
BstYI	RGATCY	2 cut(s)	47, 373
BstZ17I	GTATAC	1 cut(s)	1028
Bsu15I	ATCGAT	1 cut(s)	1893
BsuI	GTATCC	2 cut(s)	1217, 1445
BsuRI	GGCC	6 cut(s)	693, 1318, 1533, 2068, 2119, 2442
BsuTUI	ATCGAT	1 cut(s)	1893
BtgI	CCRYGG	2 cut(s)	276, 2443
BtsCI	GGATG	4 cut(s)	163, 732, 1974, 2615
BtsI	GCAGTG	2 cut(s)	363, 2337
BtsIMutI	CAGTG	8 cut(s)	232, 363, 498, 948, 1131, 1139, 1884, 2337
BtuMI	TCGCGA	1 cut(s)	1410
BveI	ACCTGC	1 cut(s)	79
Cac8I	GCNNGC	4 cut(s)	392, 549, 1088, 1840
CaiI	CAGNNNCTG	2 cut(s)	1096, 1622
CciI	TCATGA	1 cut(s)	211
Cfr10I	RCCGGY	3 cut(s)	689, 1124, 1278
Cfr13I	GGNCC	3 cut(s)	290, 1136, 2117
ClaI	ATCGAT	1 cut(s)	1893
CspAI	ACCGGT	2 cut(s)	1124, 1278
DdeI	CTNAG	6 cut(s)	182, 236, 801, 1263, 2089, 2643
DriI	GACNNNNNGTC	1 cut(s)	1417
EaeI	YGGCCR	2 cut(s)	1531, 2440
Eam1104I	CTCTTC	2 cut(s)	843, 2087
Eam1105I	GACNNNNNGTC	1 cut(s)	1417
EarI	CTCTTC	2 cut(s)	843, 2087
Ecl136II	GAGCTC	1 cut(s)	830
Eco130I	CCWWGG	1 cut(s)	2443
Eco147I	AGGCCT	1 cut(s)	2068
Eco24I	GRGCYC	1 cut(s)	832
Eco31I	GGTCTC	3 cut(s)	606, 1133, 1167
Eco32I	GATATC	2 cut(s)	1186, 1905
Eco47I	GGWCC	2 cut(s)	290, 1136
Eco53kI	GAGCTC	1 cut(s)	830
Eco57I	CTGAAG	3 cut(s)	1560, 2207, 2427
Eco72I	CACGTG	1 cut(s)	346
Eco88I	CYCGRG	4 cut(s)	609, 657, 682, 1341
EcoICRI	GAGCTC	1 cut(s)	830
EcoRI	GAATTC	1 cut(s)	2123
EcoRII	CCWGG	1 cut(s)	1318
EcoRV	GATATC	2 cut(s)	1186, 1905
EcoT14I	CCWWGG	1 cut(s)	2443
EcoT22I	ATGCAT	1 cut(s)	2235
EcoT38I	GRGCYC	1 cut(s)	832
ErhI	CCWWGG	1 cut(s)	2443
FalI	AAGNNNNNCTT	2 cut(s)	2242, 2274
FaqI	GGGAC	2 cut(s)	1339, 2331
FbaI	TGATCA	3 cut(s)	427, 508, 2413
FblI	GTMKAC	1 cut(s)	1027
Fnu4HI	GCNGC	7 cut(s)	147, 1305, 1476, 1534, 2049, 2449, 2479
FokI	GGATG	4 cut(s)	170, 739, 1961, 2602
FriOI	GRGCYC	1 cut(s)	832
Fsp4HI	GCNGC	7 cut(s)	147, 1305, 1476, 1534, 2049, 2449, 2479
GluI	GCNGC	7 cut(s)	147, 1305, 1476, 1534, 2049, 2449, 2479
GsaI	CCCAGC	1 cut(s)	2143
GsuI	CTGGAG	2 cut(s)	1341, 1810
HaeIII	GGCC	6 cut(s)	693, 1318, 1533, 2068, 2119, 2442
HapII	CCGG	5 cut(s)	690, 854, 1125, 1279, 2395
HincII	GTYRAC	3 cut(s)	976, 2187, 2679
HindII	GTYRAC	3 cut(s)	976, 2187, 2679
HindIII	AAGCTT	2 cut(s)	545, 2308
HpaI	GTTAAC	1 cut(s)	2679
HpaII	CCGG	5 cut(s)	690, 854, 1125, 1279, 2395
HphI	GGTGA	6 cut(s)	551, 1538, 1869, 1998, 2119, 2228
Hpy166II	GTNNAC	9 cut(s)	82, 771, 976, 1028, 1139, 2006, 2187, 2635, 2679
Hpy8I	GTNNAC	9 cut(s)	82, 771, 976, 1028, 1139, 2006, 2187, 2635, 2679
HpyAV	CCTTC	7 cut(s)	50, 709, 818, 1185, 1672, 1676, 2248
HpyCH4IV	ACGT	3 cut(s)	345, 2486, 2548
HpyF10VI	GCNNNNNNNGC	5 cut(s)	98, 143, 938, 1786, 2448
HpyF3I	CTNAG	6 cut(s)	182, 236, 801, 1263, 2089, 2643
HpySE526I	ACGT	3 cut(s)	345, 2486, 2548
KpnI	GGTACC	1 cut(s)	1002
Ksp22I	TGATCA	3 cut(s)	427, 508, 2413
KspAI	GTTAAC	1 cut(s)	2679
LmnI	GCTCC	5 cut(s)	59, 89, 248, 827, 1752
Lsp1109I	GCAGC	5 cut(s)	133, 1291, 1487, 2035, 2460
LweI	GCATC	2 cut(s)	71, 249
MaeII	ACGT	3 cut(s)	345, 2486, 2548
MaeIII	GTNAC	3 cut(s)	351, 526, 1419
MflI	RGATCY	2 cut(s)	47, 373
MhlI	GDGCHC	4 cut(s)	253, 832, 1260, 1749
MlsI	TGGCCA	1 cut(s)	2442
MluNI	TGGCCA	1 cut(s)	2442
MlyI	GAGTC	6 cut(s)	132, 275, 662, 809, 1613, 2156
MmeI	TCCRAC	3 cut(s)	455, 623, 1774
Mox20I	TGGCCA	1 cut(s)	2442
Mph1103I	ATGCAT	1 cut(s)	2235
MscI	TGGCCA	1 cut(s)	2442
MseI	TTAA	9 cut(s)	114, 141, 621, 1067, 1077, 1191, 1376, 2678, 2686
MslI	CAYNNNNRTG	1 cut(s)	2180
Msp20I	TGGCCA	1 cut(s)	2442
MspI	CCGG	5 cut(s)	690, 854, 1125, 1279, 2395
MspR9I	CCNGG	1 cut(s)	1320
Mva1269I	GAATGC	3 cut(s)	169, 177, 448
MvaI	CCWGG	1 cut(s)	1320
MvnI	CGCG	1 cut(s)	1410
MwoI	GCNNNNNNNGC	5 cut(s)	98, 143, 938, 1786, 2448
NcoI	CCATGG	1 cut(s)	2443
NheI	GCTAGC	1 cut(s)	1838
NlaIV	GGNNCC	1 cut(s)	1000
NmeAIII	GCCGAG	1 cut(s)	175
NmuCI	GTSAC	1 cut(s)	1419
NruI	TCGCGA	1 cut(s)	1410
NsiI	ATGCAT	1 cut(s)	2235
NspI	RCATGY	2 cut(s)	2104, 2346
NspV	TTCGAA	1 cut(s)	218
PaeR7I	CTCGAG	3 cut(s)	609, 657, 682
PagI	TCATGA	1 cut(s)	211
PceI	AGGCCT	1 cut(s)	2068
PctI	GAATGC	3 cut(s)	169, 177, 448
PflMI	CCANNNNNTGG	1 cut(s)	1364
PinAI	ACCGGT	2 cut(s)	1124, 1278
PkrI	GCNGC	7 cut(s)	148, 1306, 1477, 1535, 2050, 2450, 2480
PleI	GAGTC	6 cut(s)	131, 274, 661, 809, 1613, 2156
PmaCI	CACGTG	1 cut(s)	346
PmlI	CACGTG	1 cut(s)	346
PpsI	GAGTC	6 cut(s)	131, 274, 661, 809, 1613, 2156
Ppu21I	YACGTR	1 cut(s)	346
PshBI	ATTAAT	2 cut(s)	114, 1376
PsiI	TTATAA	1 cut(s)	1808
Psp124BI	GAGCTC	1 cut(s)	832
Psp6I	CCWGG	1 cut(s)	1318
PspCI	CACGTG	1 cut(s)	346
PspFI	CCCAGC	1 cut(s)	2139
PspGI	CCWGG	1 cut(s)	1318
PspN4I	GGNNCC	1 cut(s)	1000
PspPI	GGNCC	3 cut(s)	290, 1136, 2117
PsrI	GAACNNNNNNTAC	2 cut(s)	1532, 1564
PstI	CTGCAG	1 cut(s)	396
PstNI	CAGNNNCTG	2 cut(s)	1096, 1622
PsuI	RGATCY	2 cut(s)	47, 373
RruI	TCGCGA	1 cut(s)	1410
RseI	CAYNNNNRTG	1 cut(s)	2180
SacI	GAGCTC	1 cut(s)	832
SaqAI	TTAA	9 cut(s)	114, 141, 621, 1067, 1077, 1191, 1376, 2678, 2686
SatI	GCNGC	7 cut(s)	147, 1305, 1476, 1534, 2049, 2449, 2479
Sau96I	GGNCC	3 cut(s)	290, 1136, 2117
ScaI	AGTACT	2 cut(s)	585, 1590
SchI	GAGTC	6 cut(s)	132, 275, 662, 809, 1613, 2156
ScrFI	CCNGG	1 cut(s)	1320
SduI	GDGCHC	4 cut(s)	253, 832, 1260, 1749
SfaNI	GCATC	2 cut(s)	71, 249
SfcI	CTRYAG	5 cut(s)	223, 282, 392, 1639, 1920
Sfr274I	CTCGAG	3 cut(s)	609, 657, 682
SfuI	TTCGAA	1 cut(s)	218
SinI	GGWCC	2 cut(s)	290, 1136
SlaI	CTCGAG	3 cut(s)	609, 657, 682
SmiMI	CAYNNNNRTG	1 cut(s)	2180
SmlI	CTYRAG	4 cut(s)	609, 657, 682, 2030
SmoI	CTYRAG	4 cut(s)	609, 657, 682, 2030
SseBI	AGGCCT	1 cut(s)	2068
SsiI	CCGC	3 cut(s)	845, 1534, 2479
SstI	GAGCTC	1 cut(s)	832
StuI	AGGCCT	1 cut(s)	2068
StyD4I	CCNGG	1 cut(s)	1318
StyI	CCWWGG	1 cut(s)	2443
TaiI	ACGT	3 cut(s)	348, 2489, 2551
TaqI	TCGA	9 cut(s)	218, 610, 658, 683, 822, 1893, 1914, 1962, 2283
TatI	WGTACW	6 cut(s)	583, 1588, 1596, 1861, 2612, 2626
TauI	GCSGC	2 cut(s)	1536, 2481
Tru1I	TTAA	9 cut(s)	114, 141, 621, 1067, 1077, 1191, 1376, 2678, 2686
Tru9I	TTAA	9 cut(s)	114, 141, 621, 1067, 1077, 1191, 1376, 2678, 2686
TscAI	CASTG	8 cut(s)	232, 370, 505, 955, 1138, 1146, 1891, 2344
TseFI	GTSAC	1 cut(s)	1419
TseI	GCWGC	5 cut(s)	146, 1304, 1475, 2048, 2448
Tsp45I	GTSAC	1 cut(s)	1419
TspRI	CASTG	8 cut(s)	232, 370, 505, 955, 1138, 1146, 1891, 2344
Van91I	CCANNNNNTGG	1 cut(s)	1364
VpaK11BI	GGWCC	2 cut(s)	290, 1136
VspI	ATTAAT	2 cut(s)	114, 1376
XapI	RAATTY	2 cut(s)	194, 2123
XbaI	TCTAGA	1 cut(s)	1985
XceI	RCATGY	2 cut(s)	2104, 2346
XhoI	CTCGAG	3 cut(s)	609, 657, 682
XmiI	GTMKAC	1 cut(s)	1027
ZrmI	AGTACT	2 cut(s)	585, 1590
Zsp2I	ATGCAT	1 cut(s)	2235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.