Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003979

Physical Location & Seq

Genomic Coordinates Forward (+)

27044104 .. 27047750

Gene Structure 3647 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1139700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2829 bp

ATGAATCTAGTTTGGCTTGTGTCGATACTCCTTATCTGCAGACAAGGCTCCGTAGAAGCGGCCTCAAGACCTGCTGTCGTGAATGTTGGCGCAATGTTTGCAGCAGGCAGCATCAACGGAAGAGTCTTGAAGATTGCCATTGAGGCGGCTAGGAACGATGTCAATTCTGATCCAAGCATTCTTCGTAAAACTAAATTATCCATATCTTTTCATAATTCCAACTACAGCGGATTTCTTGGACTCGTTGGAGCGTTAAGGTACATGGAGTCTGATACATTTGCTATAATTGGTCCACAAAATTCTGGGATGGCTCACATACTCTCACATCTTGCCAATGAGCTACGTGTCCCTTTATTGTCAGTCACAGCGCTGGACCCCACCCTAGCAAGTCTGCAGTACCCTTTCTTTGTTCAAACTGCACCGAATGATCAATTCCAGATGGCTGCTATTGCAGACATTGTTAGTTATTTCGGTTGGTCAGAGGTGGTCGCGATTTTCACAGACGATGAAAACAGCCGAAATGGTGTTGCTGCATTAGGTGAGAAACTTGCAGAAAAACGCCACAAGATTTCATACAAGGCAGTACTTCCACCTGACCCGGAACCAACTCGAGAAGATGTAAAAAATCTGTTGATAAAGGTTCGAATGGTGGAGTCTCGAGTGATTGTTATACACACATTCTCCACATCTGGTCTCCTGGTTTTCGATGTTGCTAAAGAACTTGGGATGATGGAGAGTGGATATGTTTGGATAGCCGCAACTTGGCTGTCTACTGTTTTAGATTCAACTTCACCTCTTTCTTCGAAAACCGCCAGCTCCATCCAAGGTGCTCTGACTCTTCGTCCACACACGCCGGATTCTAAGCGAAAAAAGGATTTCATATCAAGGTGGAACGAGTTGAGTAATGGCTCTATTGGGTTGAACCCTTACGGTCTGTATGCCTATGATACAGTTTGGATGCTTGCCCGTGCTATAGATTTGCTTTTGGAACAAGGAAGCAGCATTGCCTTTTCCAACATTACTAGTATAGGACCTCTTGGCGGTCTTAAAGGAGGGACTTTGAACCTGGGTGCATTGAGCATTTTTCAGGGTGGGAAGCAGTTGCTGGACAATATTTTGCGGACAAATACAACCGGTCTGACTGGTCGGGTAGCATTTCATCCAGATAGATCGCCATTGAATCCTTCATATGAAATCATTAACACAATCGCAAATGGATATCAAAGAATTGGATACTGGTCCAACTACTCTGGTATATCTCTTGTGGCCCCTGAGAAATTATCCAACCGGTCTAATGCGAACCAACGTTTGGACACTGTTGTATGGCCCGGAGGGACAACAGTTAAGCCTCGGGGGTGGGTTTTTCCAAACAATGGAAAGAAATTGAGAATTGGAGTGCCAAATCGAGTCAGCTTTCAAGGCTTTGTGTCACGAACAAGTAATACTGATGTAGTTGAAGGATACTGCATCGATATATTCATTGCAGCCATAAATTTGCTTCCTTATGCAGTTCCATATAAGTTTGTTCTGTTTGGTGATGGCCTCAAGAACCCTAGCTACAACGAGCTCGTAAACATGGTTGCTTCAGGTAAGTTTGATGCTGCGGTAGGTGACATTGCAATCGTCACAAACCGGACAAAGATTGTGGATTTCACTCAGCCATATATAGAGTCGGGGCTAGTTGTGGTGGCTCCAGTTAGGGATTCGAATTCAGCTTGGGCATTCTTAAGGCCATTTTCTTCATCAATGTGGGGTGTAACAGCATCCTTCTTCATAATCATTGGATTAGTGATATGGATTCTTGAACATAGAAAAAACGATGAGTTCCGAGGCCCTCCTAGGAAACAGATTGTCACAATTTTATGGTTTAGCTTCTCTACCATGTTTTTTGCTCACAGAGAGAACACCTTAACCACGCTAGGCCGCATGGTGCTGATCATATGGCTTTTTGTAGTTCTAGTGATCAACTCAAGCTACACAGCTAGCCTGACATCAATGCTCACAGTAAAACAACTATCATCCCCCATCACCGGCATCGATACCTTGATAACTAGCACAGAATCTATAGGATTCCAAATCGGATCTTTTGCTCAGAACTATTTGGTTGAGCAGCTCGACATCCCAAAGTCCAGACTAGTTCCTCTTGGCTCGCCAGAAGCTTATGCAACCGCCCTTAAGGATAAAACTGTTGCTGCTGTGGTTGACGAAAGAGCATATATGGAACTCTTCCTCTCAGAAAACTGCAAGTTCTCAATTAGGGGGGCAGAATTCACCAAAAGTGGGTGGGGATTTGCATTTCCAAGAGACTCTCCTTTCGCAATTGACATGTCAACTGCCATCCTCACTCTATCTGAAAATGGTGATCTCCAGAAAATTTATAACAAATGGCTCTCCAGAAAGATTTGTGCTTCAGAGAGCTCCAGCATCGTATCCGACCAGCTTCAACTACAAAGCTTCTGGGGGCTATTCCTTATCGCCGGCATTGTATCTTTTATTGCTCTACTCATTTACCTGTTTACCACGCTACACCAGTACAAACGCCATACCCCCGAAGCTGAAGATCAAGCCGAGCCTTCGAGCCAAGAAAACATCAACTCTCACTCTATCTTAACACGCCTCCTCACGTTCTTGTCCTTCGTCGACAAAAAGGTAGATGAATCGAAGAAAAATAAGCTGAAAAGAAAGCGCAAGGAAAGCGTAGTACCAAATGGTCCAAATGTGATTGAAAATGAAGATGATCAATCAGTAAGGAATGCTTCCAAGAGAATACAAATCGACAACCCTCAAGAGATGCGTAATGCTGACAATGACATTTGGCTAACTCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

943

Amino Acids

104.2

Weight (kDa)

8.91

Isoelectric Point (pI)

37.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	36 - 325	8.7e-13	Periplasmic binding protein
ANF_receptor	PF01094	44 - 403	9.4e-86	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	479 - 564	7.7e-10	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	518 - 798	3e-11	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	581 - 828	2.1e-37	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003979)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17260 AT4G35290 AT4G35290 AT4G35290 AT4G35290 AT4G35290 AT4G35290
fragaria_vesca	FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810
malus_domestica	MD05G1139700.v1.1 MD10G1141900.v1.1
prunus_persica	Prupe.8G180400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280931
rosa_laevigata	RLG00000013015
rosa_multiflora	Rmu_ssc0000288.1_g000001
rosa_roxburghii	Rroxscaffold_7G00187480
rosa_rugosa	Rorug06G0136800 Rorug06G0136900 Rorug06G0137000 Rorug06G0137000 Rorug06G0137000
rosa_samantha	Rh6AG249300 Rh6CG252000 Rh6DG243800
rosa_wichuraiana	Rw6G005260 Rw6G021570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2379
Acc36I	ACCTGC	1 cut(s)	79
AccB7I	CCANNNNNTGG	2 cut(s)	1309, 1373
AccI	GTMKAC	2 cut(s)	770, 2640
AccII	CGCG	1 cut(s)	491
AclI	AACGTT	1 cut(s)	1306
AclWI	GGATC	2 cut(s)	164, 2089
AcsI	RAATTY	5 cut(s)	298, 1492, 1708, 2267, 2373
AcuI	CTGAAG	3 cut(s)	1569, 2394, 2577
AfaI	GTAC	5 cut(s)	260, 398, 585, 2534, 2703
AfeI	AGCGCT	1 cut(s)	369
AfiI	CCNNNNNNNGG	6 cut(s)	762, 1040, 1309, 1373, 2030, 2280
AflII	CTTAAG	2 cut(s)	1726, 2174
AflIII	ACRYGT	2 cut(s)	343, 2325
AgeI	ACCGGT	2 cut(s)	1133, 1287
AhdI	GACNNNNNGTC	2 cut(s)	74, 840
AhlI	ACTAGT	2 cut(s)	1022, 2134
AjnI	CCWGG	2 cut(s)	696, 1065
AjuI	GAANNNNNNNTTGG	2 cut(s)	1292, 1324
Alw21I	GWGCWC	3 cut(s)	832, 1569, 2420
Alw26I	GTCTC	3 cut(s)	660, 698, 2298
AlwI	GGATC	2 cut(s)	164, 2089
AlwNI	CAGNNNCTG	1 cut(s)	1105
Ama87I	CYCGRG	3 cut(s)	609, 657, 1350
Aor51HI	AGCGCT	1 cut(s)	369
AoxI	GGCC	7 cut(s)	60, 1266, 1325, 1540, 1730, 1831, 1921
ApeKI	GCWGC	9 cut(s)	101, 108, 443, 530, 999, 1484, 1601, 2110, 2192
ApoI	RAATTY	5 cut(s)	298, 1492, 1708, 2267, 2373
ArsI	GACNNNNNNTTYG	2 cut(s)	2297, 2329
AsiGI	ACCGGT	2 cut(s)	1133, 1287
AspA2I	CCTAGG	1 cut(s)	1838
AspLEI	GCGC	3 cut(s)	92, 370, 2688
AspS9I	GGNCC	8 cut(s)	290, 373, 1031, 1239, 1267, 1326, 1832, 2711
AsuC2I	CCSGG	2 cut(s)	599, 1329
AsuHPI	GGTGA	7 cut(s)	551, 783, 1547, 1622, 2020, 2263, 2372
AsuII	TTCGAA	3 cut(s)	643, 803, 1706
AsuNHI	GCTAGC	1 cut(s)	1982
AvaI	CYCGRG	3 cut(s)	609, 657, 1350
AvaII	GGWCC	5 cut(s)	290, 373, 1031, 1239, 2711
AvrII	CCTAGG	1 cut(s)	1838
BanII	GRGCYC	2 cut(s)	1569, 2420
Bbv12I	GWGCWC	3 cut(s)	832, 1569, 2420
BbvI	GCAGC	9 cut(s)	113, 120, 430, 517, 1011, 1496, 1588, 2122, 2179
BccI	CCATC	7 cut(s)	301, 433, 724, 827, 1532, 2033, 2345
BciT130I	CCWGG	2 cut(s)	698, 1067
BciVI	GTATCC	3 cut(s)	1226, 1454, 2440
BclI	TGATCA	4 cut(s)	427, 1935, 1962, 2737
BcnI	CCSGG	2 cut(s)	599, 1329
BcoDI	GTCTC	3 cut(s)	660, 698, 2298
BcuI	ACTAGT	2 cut(s)	1022, 2134
BfmI	CTRYAG	5 cut(s)	37, 223, 392, 972, 2064
BfoI	RGCGCY	1 cut(s)	371
BfrI	CTTAAG	2 cut(s)	1726, 2174
BfuAI	ACCTGC	1 cut(s)	79
BfuI	GTATCC	3 cut(s)	1226, 1454, 2440
BglI	GCCNNNNNGGC	1 cut(s)	143
BlnI	CCTAGG	1 cut(s)	1838
BmcAI	AGTACT	1 cut(s)	585
Bme1390I	CCNGG	4 cut(s)	599, 698, 1067, 1329
Bme18I	GGWCC	5 cut(s)	290, 373, 1031, 1239, 2711
BmeRI	GACNNNNNGTC	2 cut(s)	74, 840
BmeT110I	CYCGRG	3 cut(s)	609, 657, 1350
BmgT120I	GGNCC	8 cut(s)	290, 373, 1031, 1239, 1267, 1326, 1832, 2711
BmiI	GGNNCC	5 cut(s)	49, 375, 603, 1269, 1692
BmrFI	CCNGG	4 cut(s)	599, 698, 1067, 1329
BmsI	GCATC	8 cut(s)	120, 948, 1476, 1588, 1772, 2043, 2433, 2781
BmtI	GCTAGC	1 cut(s)	1986
BpmI	CTGGAG	4 cut(s)	1677, 2351, 2377, 2404
Bpu14I	TTCGAA	3 cut(s)	643, 803, 1706
BpuEI	CTTGAG	4 cut(s)	49, 1529, 1954, 2769
BpuMI	CCSGG	2 cut(s)	599, 1329
Bsa29I	ATCGAT	2 cut(s)	1470, 2037
BsaAI	YACGTR	1 cut(s)	344
BsaI	GGTCTC	1 cut(s)	698
BsaJI	CCNNGG	5 cut(s)	823, 1066, 1349, 1828, 1838
BsaWI	WCCGGW	3 cut(s)	1133, 1287, 1632
BsaXI	ACNNNNNCTCC	2 cut(s)	665, 695
Bsc4I	CCNNNNNNNGG	6 cut(s)	762, 1040, 1309, 1373, 2030, 2280
Bse118I	RCCGGY	4 cut(s)	1133, 1287, 2030, 2477
Bse1I	ACTGG	4 cut(s)	1147, 1241, 1694, 2530
Bse3DI	GCAATG	4 cut(s)	99, 1002, 1479, 1614
BseBI	CCWGG	2 cut(s)	698, 1067
BseCI	ATCGAT	2 cut(s)	1470, 2037
BseDI	CCNNGG	5 cut(s)	823, 1066, 1349, 1828, 1838
BseGI	GGATG	9 cut(s)	312, 732, 819, 963, 1159, 1763, 2018, 2118, 2337
BseLI	CCNNNNNNNGG	6 cut(s)	762, 1040, 1309, 1373, 2030, 2280
BseMI	GCAATG	4 cut(s)	99, 1002, 1479, 1614
BseMII	CTCAG	4 cut(s)	1263, 1670, 2105, 2247
BseNI	ACTGG	4 cut(s)	1147, 1241, 1694, 2530
BseRI	GAGGAG	1 cut(s)	2609
BseXI	GCAGC	9 cut(s)	113, 120, 430, 517, 1011, 1496, 1588, 2122, 2179
BsgI	GTGCAG	1 cut(s)	402
Bsh1236I	CGCG	1 cut(s)	491
BshFI	GGCC	7 cut(s)	62, 1268, 1327, 1542, 1732, 1833, 1923
BshTI	ACCGGT	2 cut(s)	1133, 1287
BshVI	ATCGAT	2 cut(s)	1470, 2037
BsiHKAI	GWGCWC	3 cut(s)	832, 1569, 2420
BsiHKCI	CYCGRG	3 cut(s)	609, 657, 1350
BsiSI	CCGG	8 cut(s)	599, 854, 1134, 1288, 1329, 1633, 2031, 2478
BslFI	GGGAC	3 cut(s)	332, 1069, 1348
BslI	CCNNNNNNNGG	6 cut(s)	762, 1040, 1309, 1373, 2030, 2280
BsmAI	GTCTC	3 cut(s)	660, 698, 2298
BsmFI	GGGAC	3 cut(s)	332, 1069, 1348
BsmI	GAATGC	3 cut(s)	177, 1721, 2758
BsnI	GGCC	7 cut(s)	62, 1268, 1327, 1542, 1732, 1833, 1923
Bso31I	GGTCTC	1 cut(s)	698
BsoBI	CYCGRG	3 cut(s)	609, 657, 1350
Bsp119I	TTCGAA	3 cut(s)	643, 803, 1706
Bsp1286I	GDGCHC	3 cut(s)	832, 1569, 2420
Bsp143I	GATC	9 cut(s)	169, 427, 1169, 1935, 1962, 2081, 2362, 2560, 2737
Bsp68I	TCGCGA	1 cut(s)	491
BspANI	GGCC	7 cut(s)	62, 1268, 1327, 1542, 1732, 1833, 1923
BspCNI	CTCAG	4 cut(s)	1264, 1669, 2104, 2246
BspDI	ATCGAT	2 cut(s)	1470, 2037
BspFNI	CGCG	1 cut(s)	491
BspLI	GGNNCC	5 cut(s)	49, 375, 603, 1269, 1692
BspMAI	CTGCAG	2 cut(s)	41, 396
BspMI	ACCTGC	1 cut(s)	79
BspOI	GCTAGC	1 cut(s)	1986
BspPI	GGATC	2 cut(s)	164, 2089
BspT104I	TTCGAA	3 cut(s)	643, 803, 1706
BspTI	CTTAAG	2 cut(s)	1726, 2174
BspTNI	GGTCTC	1 cut(s)	698
BsrDI	GCAATG	4 cut(s)	99, 1002, 1479, 1614
BsrFI	RCCGGY	4 cut(s)	1133, 1287, 2030, 2477
BsrI	ACTGG	4 cut(s)	1147, 1241, 1694, 2530
BssAI	RCCGGY	4 cut(s)	1133, 1287, 2030, 2477
BssECI	CCNNGG	5 cut(s)	823, 1066, 1349, 1828, 1838
BssMI	GATC	9 cut(s)	169, 427, 1169, 1935, 1962, 2081, 2362, 2560, 2737
BssT1I	CCWWGG	2 cut(s)	823, 1838
Bst2UI	CCWGG	2 cut(s)	698, 1067
Bst4CI	ACNGT	7 cut(s)	775, 932, 952, 1318, 1342, 2005, 2188
Bst6I	CTCTTC	3 cut(s)	115, 843, 2231
BstAFI	CTTAAG	2 cut(s)	1726, 2174
BstAPI	GCANNNNNTGC	1 cut(s)	98
BstBAI	YACGTR	1 cut(s)	344
BstBI	TTCGAA	3 cut(s)	643, 803, 1706
BstC8I	GCNNGC	6 cut(s)	106, 814, 963, 1984, 2150, 2479
BstDEI	CTNAG	5 cut(s)	861, 1272, 1656, 2091, 2233
BstF5I	GGATG	9 cut(s)	312, 732, 819, 963, 1159, 1763, 2018, 2118, 2337
BstFNI	CGCG	1 cut(s)	491
BstH2I	RGCGCY	1 cut(s)	371
BstHHI	GCGC	3 cut(s)	92, 370, 2688
BstKTI	GATC	9 cut(s)	172, 430, 1172, 1938, 1965, 2084, 2365, 2563, 2740
BstMAI	GTCTC	3 cut(s)	660, 698, 2298
BstMBI	GATC	9 cut(s)	169, 427, 1169, 1935, 1962, 2081, 2362, 2560, 2737
BstMWI	GCNNNNNNNGC	6 cut(s)	45, 98, 143, 449, 1419, 2694
BstNI	CCWGG	2 cut(s)	698, 1067
BstNSI	RCATGY	1 cut(s)	2329
BstSCI	CCNGG	4 cut(s)	597, 696, 1065, 1327
BstSFI	CTRYAG	5 cut(s)	37, 223, 392, 972, 2064
BstUI	CGCG	1 cut(s)	491
BstV1I	GCAGC	9 cut(s)	113, 120, 430, 517, 1011, 1496, 1588, 2122, 2179
BstX2I	RGATCY	1 cut(s)	2081
BstYI	RGATCY	1 cut(s)	2081
Bsu15I	ATCGAT	2 cut(s)	1470, 2037
BsuI	GTATCC	3 cut(s)	1226, 1454, 2440
BsuRI	GGCC	7 cut(s)	62, 1268, 1327, 1542, 1732, 1833, 1923
BsuTUI	ATCGAT	2 cut(s)	1470, 2037
BtsCI	GGATG	9 cut(s)	312, 732, 819, 963, 1159, 1763, 2018, 2118, 2337
BtsIMutI	CAGTG	1 cut(s)	1314
BtuMI	TCGCGA	1 cut(s)	491
BveI	ACCTGC	1 cut(s)	79
Cac8I	GCNNGC	6 cut(s)	106, 814, 963, 1984, 2150, 2479
CaiI	CAGNNNCTG	1 cut(s)	1105
CfoI	GCGC	3 cut(s)	92, 370, 2688
Cfr10I	RCCGGY	4 cut(s)	1133, 1287, 2030, 2477
Cfr13I	GGNCC	8 cut(s)	290, 373, 1031, 1239, 1267, 1326, 1832, 2711
ClaI	ATCGAT	2 cut(s)	1470, 2037
Csp6I	GTAC	5 cut(s)	259, 397, 584, 2533, 2702
CspAI	ACCGGT	2 cut(s)	1133, 1287
CspCI	CAANNNNNGTGG	2 cut(s)	1626, 1661
CviAII	CATG	5 cut(s)	262, 1576, 1882, 1927, 2326
CviQI	GTAC	5 cut(s)	259, 397, 584, 2533, 2702
DdeI	CTNAG	5 cut(s)	861, 1272, 1656, 2091, 2233
DpnI	GATC	9 cut(s)	171, 429, 1171, 1937, 1964, 2083, 2364, 2562, 2739
DpnII	GATC	9 cut(s)	169, 427, 1169, 1935, 1962, 2081, 2362, 2560, 2737
DriI	GACNNNNNGTC	2 cut(s)	74, 840
Eam1104I	CTCTTC	3 cut(s)	115, 843, 2231
Eam1105I	GACNNNNNGTC	2 cut(s)	74, 840
EarI	CTCTTC	3 cut(s)	115, 843, 2231
Ecl136II	GAGCTC	2 cut(s)	1567, 2418
Eco130I	CCWWGG	2 cut(s)	823, 1838
Eco24I	GRGCYC	2 cut(s)	1569, 2420
Eco31I	GGTCTC	1 cut(s)	698
Eco32I	GATATC	1 cut(s)	1220
Eco47I	GGWCC	5 cut(s)	290, 373, 1031, 1239, 2711
Eco47III	AGCGCT	1 cut(s)	369
Eco53kI	GAGCTC	2 cut(s)	1567, 2418
Eco57I	CTGAAG	3 cut(s)	1569, 2394, 2577
Eco88I	CYCGRG	3 cut(s)	609, 657, 1350
EcoICRI	GAGCTC	2 cut(s)	1567, 2418
EcoO109I	RGGNCCY	2 cut(s)	1031, 1832
EcoRI	GAATTC	2 cut(s)	1708, 2267
EcoRII	CCWGG	2 cut(s)	696, 1065
EcoRV	GATATC	1 cut(s)	1220
EcoT14I	CCWWGG	2 cut(s)	823, 1838
EcoT38I	GRGCYC	2 cut(s)	1569, 2420
ErhI	CCWWGG	2 cut(s)	823, 1838
FaeI	CATG	5 cut(s)	265, 1579, 1885, 1930, 2329
FalI	AAGNNNNNCTT	2 cut(s)	2740, 2772
FaqI	GGGAC	3 cut(s)	332, 1069, 1348
FatI	CATG	5 cut(s)	261, 1575, 1881, 1926, 2325
FauNDI	CATATG	2 cut(s)	1189, 1940
FbaI	TGATCA	4 cut(s)	427, 1935, 1962, 2737
FblI	GTMKAC	2 cut(s)	770, 2640
FokI	GGATG	9 cut(s)	319, 739, 806, 970, 1146, 1750, 2005, 2105, 2324
FriOI	GRGCYC	2 cut(s)	1569, 2420
GlaI	GCGC	3 cut(s)	91, 369, 2687
GsuI	CTGGAG	4 cut(s)	1677, 2351, 2377, 2404
HaeII	RGCGCY	1 cut(s)	371
HaeIII	GGCC	7 cut(s)	62, 1268, 1327, 1542, 1732, 1833, 1923
HapII	CCGG	8 cut(s)	599, 854, 1134, 1288, 1329, 1633, 2031, 2478
HhaI	GCGC	3 cut(s)	92, 370, 2688
Hin1II	CATG	5 cut(s)	265, 1579, 1885, 1930, 2329
Hin6I	GCGC	3 cut(s)	90, 368, 2686
HinP1I	GCGC	3 cut(s)	90, 368, 2686
HincII	GTYRAC	3 cut(s)	2203, 2331, 2641
HindII	GTYRAC	3 cut(s)	2203, 2331, 2641
HindIII	AAGCTT	2 cut(s)	2157, 2452
HpaII	CCGG	8 cut(s)	599, 854, 1134, 1288, 1329, 1633, 2031, 2478
HphI	GGTGA	7 cut(s)	551, 783, 1547, 1622, 2020, 2263, 2372
Hpy166II	GTNNAC	8 cut(s)	293, 771, 845, 1573, 2203, 2331, 2517, 2641
Hpy8I	GTNNAC	8 cut(s)	293, 771, 845, 1573, 2203, 2331, 2517, 2641
Hpy99I	CGWCG	1 cut(s)	2642
HpyAV	CCTTC	5 cut(s)	1194, 1451, 1777, 2583, 2644
HpyCH4III	ACNGT	7 cut(s)	775, 932, 952, 1318, 1342, 2005, 2188
HpyCH4IV	ACGT	3 cut(s)	343, 1306, 2624
HpyF10VI	GCNNNNNNNGC	6 cut(s)	45, 98, 143, 449, 1419, 2694
HpyF3I	CTNAG	5 cut(s)	861, 1272, 1656, 2091, 2233
HpySE526I	ACGT	3 cut(s)	343, 1306, 2624
Hsp92II	CATG	5 cut(s)	265, 1579, 1885, 1930, 2329
HspAI	GCGC	3 cut(s)	90, 368, 2686
KroI	GCCGGC	1 cut(s)	2477
KroNI	GCCGGC	1 cut(s)	2479
Ksp22I	TGATCA	4 cut(s)	427, 1935, 1962, 2737
Kzo9I	GATC	9 cut(s)	169, 427, 1169, 1935, 1962, 2081, 2362, 2560, 2737
LmnI	GCTCC	5 cut(s)	53, 248, 821, 1696, 2423
Lsp1109I	GCAGC	9 cut(s)	113, 120, 430, 517, 1011, 1496, 1588, 2122, 2179
LweI	GCATC	8 cut(s)	120, 948, 1476, 1588, 1772, 2043, 2433, 2781
MaeII	ACGT	3 cut(s)	343, 1306, 2624
MaeIII	GTNAC	6 cut(s)	361, 1428, 1610, 1624, 1756, 1852
MalI	GATC	9 cut(s)	171, 429, 1171, 1937, 1964, 2083, 2364, 2562, 2739
MboI	GATC	9 cut(s)	169, 427, 1169, 1935, 1962, 2081, 2362, 2560, 2737
MfeI	CAATTG	1 cut(s)	2319
MflI	RGATCY	1 cut(s)	2081
MhlI	GDGCHC	3 cut(s)	832, 1569, 2420
MlyI	GAGTC	8 cut(s)	132, 234, 275, 662, 829, 1416, 1679, 2300
MmeI	TCCRAC	6 cut(s)	226, 243, 1038, 1266, 1308, 2457
MroNI	GCCGGC	1 cut(s)	2477
MseI	TTAA	9 cut(s)	254, 1047, 1200, 1344, 1727, 1910, 2175, 2609, 2827
MslI	CAYNNNNRTG	1 cut(s)	1747
MspA1I	CMGCKG	1 cut(s)	228
MspCI	CTTAAG	2 cut(s)	1726, 2174
MspI	CCGG	8 cut(s)	599, 854, 1134, 1288, 1329, 1633, 2031, 2478
MspR9I	CCNGG	4 cut(s)	599, 698, 1067, 1329
MunI	CAATTG	1 cut(s)	2319
Mva1269I	GAATGC	3 cut(s)	177, 1721, 2758
MvaI	CCWGG	2 cut(s)	698, 1067
MvnI	CGCG	1 cut(s)	491
MwoI	GCNNNNNNNGC	6 cut(s)	45, 98, 143, 449, 1419, 2694
NaeI	GCCGGC	1 cut(s)	2479
NciI	CCSGG	2 cut(s)	599, 1329
NdeI	CATATG	2 cut(s)	1189, 1940
NdeII	GATC	9 cut(s)	169, 427, 1169, 1935, 1962, 2081, 2362, 2560, 2737
NgoMIV	GCCGGC	1 cut(s)	2477
NheI	GCTAGC	1 cut(s)	1982
NlaIII	CATG	5 cut(s)	265, 1579, 1885, 1930, 2329
NlaIV	GGNNCC	5 cut(s)	49, 375, 603, 1269, 1692
NmeAIII	GCCGAG	1 cut(s)	2593
NmuCI	GTSAC	5 cut(s)	361, 1428, 1610, 1624, 1852
NruI	TCGCGA	1 cut(s)	491
NspI	RCATGY	1 cut(s)	2329
NspV	TTCGAA	3 cut(s)	643, 803, 1706
PaeR7I	CTCGAG	2 cut(s)	609, 657
PciI	ACATGT	1 cut(s)	2325
PctI	GAATGC	3 cut(s)	177, 1721, 2758
PdiI	GCCGGC	1 cut(s)	2479
PfeI	GAWTC	9 cut(s)	4, 782, 857, 1180, 1703, 1798, 2060, 2070, 2657
PflMI	CCANNNNNTGG	2 cut(s)	1309, 1373
PinAI	ACCGGT	2 cut(s)	1133, 1287
PleI	GAGTC	8 cut(s)	131, 234, 274, 661, 829, 1415, 1678, 2300
PpsI	GAGTC	8 cut(s)	131, 234, 274, 661, 829, 1415, 1678, 2300
Ppu21I	YACGTR	1 cut(s)	344
PpuMI	RGGWCCY	1 cut(s)	1031
PscI	ACATGT	1 cut(s)	2325
PsiI	TTATAA	1 cut(s)	2379
Psp124BI	GAGCTC	2 cut(s)	1569, 2420
Psp1406I	AACGTT	1 cut(s)	1306
Psp5II	RGGWCCY	1 cut(s)	1031
Psp6I	CCWGG	2 cut(s)	696, 1065
PspGI	CCWGG	2 cut(s)	696, 1065
PspN4I	GGNNCC	5 cut(s)	49, 375, 603, 1269, 1692
PspPI	GGNCC	8 cut(s)	290, 373, 1031, 1239, 1267, 1326, 1832, 2711
PspPPI	RGGWCCY	1 cut(s)	1031
PsrI	GAACNNNNNNTAC	4 cut(s)	1426, 1458, 1541, 1573
PstI	CTGCAG	2 cut(s)	41, 396
PstNI	CAGNNNCTG	1 cut(s)	1105
PsuI	RGATCY	1 cut(s)	2081
RruI	TCGCGA	1 cut(s)	491
RsaI	GTAC	5 cut(s)	260, 398, 585, 2534, 2703
RsaNI	GTAC	5 cut(s)	259, 397, 584, 2533, 2702
RseI	CAYNNNNRTG	1 cut(s)	1747
SacI	GAGCTC	2 cut(s)	1569, 2420
SalI	GTCGAC	1 cut(s)	2639
SaqAI	TTAA	9 cut(s)	254, 1047, 1200, 1344, 1727, 1910, 2175, 2609, 2827
Sau3AI	GATC	9 cut(s)	169, 427, 1169, 1935, 1962, 2081, 2362, 2560, 2737
Sau96I	GGNCC	8 cut(s)	290, 373, 1031, 1239, 1267, 1326, 1832, 2711
ScaI	AGTACT	1 cut(s)	585
SchI	GAGTC	8 cut(s)	132, 234, 275, 662, 829, 1416, 1679, 2300
ScrFI	CCNGG	4 cut(s)	599, 698, 1067, 1329
SduI	GDGCHC	3 cut(s)	832, 1569, 2420
SfaNI	GCATC	8 cut(s)	120, 948, 1476, 1588, 1772, 2043, 2433, 2781
SfcI	CTRYAG	5 cut(s)	37, 223, 392, 972, 2064
Sfr274I	CTCGAG	2 cut(s)	609, 657
SfuI	TTCGAA	3 cut(s)	643, 803, 1706
SinI	GGWCC	5 cut(s)	290, 373, 1031, 1239, 2711
SlaI	CTCGAG	2 cut(s)	609, 657
SmiMI	CAYNNNNRTG	1 cut(s)	1747
SmlI	CTYRAG	8 cut(s)	64, 609, 657, 1544, 1726, 1969, 2174, 2784
SmoI	CTYRAG	8 cut(s)	64, 609, 657, 1544, 1726, 1969, 2174, 2784
SpeI	ACTAGT	2 cut(s)	1022, 2134
SspI	AATATT	1 cut(s)	1114
SstI	GAGCTC	2 cut(s)	1569, 2420
StyD4I	CCNGG	4 cut(s)	597, 696, 1065, 1327
StyI	CCWWGG	2 cut(s)	823, 1838
TaaI	ACNGT	7 cut(s)	775, 932, 952, 1318, 1342, 2005, 2188
TaiI	ACGT	3 cut(s)	346, 1309, 2627
TatI	WGTACW	2 cut(s)	583, 2532
TauI	GCSGC	4 cut(s)	62, 149, 758, 1926
TfiI	GAWTC	9 cut(s)	4, 782, 857, 1180, 1703, 1798, 2060, 2070, 2657
Tru1I	TTAA	9 cut(s)	254, 1047, 1200, 1344, 1727, 1910, 2175, 2609, 2827
Tru9I	TTAA	9 cut(s)	254, 1047, 1200, 1344, 1727, 1910, 2175, 2609, 2827
TscAI	CASTG	1 cut(s)	1321
TseFI	GTSAC	5 cut(s)	361, 1428, 1610, 1624, 1852
TseI	GCWGC	9 cut(s)	101, 108, 443, 530, 999, 1484, 1601, 2110, 2192
Tsp45I	GTSAC	5 cut(s)	361, 1428, 1610, 1624, 1852
TspGWI	ACGGA	2 cut(s)	40, 132
TspRI	CASTG	1 cut(s)	1321
Van91I	CCANNNNNTGG	2 cut(s)	1309, 1373
Vha464I	CTTAAG	2 cut(s)	1726, 2174
VpaK11BI	GGWCC	5 cut(s)	290, 373, 1031, 1239, 2711
XapI	RAATTY	5 cut(s)	298, 1492, 1708, 2267, 2373
XceI	RCATGY	1 cut(s)	2329
XhoI	CTCGAG	2 cut(s)	609, 657
XmaJI	CCTAGG	1 cut(s)	1838
XmiI	GTMKAC	2 cut(s)	770, 2640
ZrmI	AGTACT	1 cut(s)	585

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.