Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003979

Physical Location & Seq

Genomic Coordinates Forward (+)

22772212 .. 22775794

Gene Structure 3583 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1141900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2778 bp

ATGAATCTAGTTTGGCTTGTGTCGATACTCATTGTCTGCATACAAGGCTCCACAGAAGCAGCCTCAAGACCTGCTGTCGTGAATGTTGGCGCAATGTTCGCAGCTGGCAGCATCAACGGAAAAGTCTTAAAGATTGCCATTGAGGCTGCCGGGAATGATGTGAATGCTGATCCAAGCATTCTTGGCAAAACTAAATTGTCCATATCTTTTCATGATTCCAACTACAGCGGATTTCTCGGACTCATTGGAGCACTAAGGTACATGGAGTCTGATACATTCGCTATACTTGGTCCACAAAATTCTGTGATGGCTCACATACTTGCACATCTTGCCAATGACCTACGTGTCCCTTTACTGTCAGTCACGGCGCTGGACCCCACCCTAGCAAGTCTGCAGTACCCTTACTTTATTCAAACCGCACCCAATGATCAATCCCAGATGGCTGCTATTGCAGATATTGTTAGTTATTTCGGTTGGTCAGAGGTGATTGCGATTTTCACCGATGATGATAACAGCCGAAATGGTGTTGCTGCACTCGGTGACAAACTCGCCGAAAAACGCCACAAGATTTCATACAAGGCAGTGCTTCCACCTGACCCGACACCAACTCGAGAGGATGTAAAAAAATTGTTGATAAAGGTTCGAATGATGGAGTCTCGAGTGATTATTCTACACACATTCTCCAAATCTGGTCTCCTAGTTTTCGATGTGGCTAAAGAACTTGGGATGATGGAGAGTGGATATGTCTGGATAGCCACAACTTGGCTGTCCACTGTTTTGGATTCCACTTCCCCTCTTTCTTCAAAACCCGCTAGCTCCATCCAAGGCGCTCTGACTCTTCGTCCACACACGCCGGATTCTAAGCGAAAAAGAGATTTCATATCGAGGTGGAACGAGTTGAGTAATGGCACTATCGGGTTGAATCCTTATGGTCTATATGCCTATGACACAGTTTGGATGCTTGCTCATGCTATAGATTTGCTTTTGGAACGAGGGGGCAGCATTGCCTTTTCCAACATTACTAGTACAGGACCTATTGGTGGTCTTAAAGGAGGGACTTTGAATCTGGGTGCATTGAGCATTTTTCAAGGTGGGAAGCAGTTGCTGGACAATATTTTGCGAACAAATACCACCGGTCTGACTGGTCCGGTGGCATTTCATCCAGATAGGTCGCCGTTGAATCCTTCTTATGAAATCATTAACACAAACCAAAATGGATATCAAAGAACTGGATACTGGTCTAACTCCTCTGGTATATCTCTTGTGCCCCCTGAGAAATTATTCAACCGGTCTAAGGCGAACCATAGTTTGGACACTGTTGTATGGCCCGGAGGGACAACAGTTAAGCCTCGGGGGTGGGTTTTTCCAAACAATGGAGAGAAATTGAGAATTGGAGTACCAAATCGAGTCAGCTTTCGAGACTTTGTGTCACGAACAAGTGGTACTGATGTAGTTGAAGGATACTGCATTGAGATATTCATTGCAGCCATAAAGTTGCTTCCTTACGCAGTTCCATATAAGTTTGTTCTGTTTGGTGATGGCCTCAAGAACCCTAGTTACAATGAGCTCGTAAACATGGTTGCTTCAGGGAAGTTTGATGCTGCTGTGGGTGACATTGCGATCGTCACAAACCGAACAAGGACTGTGGATTTCACTCAGCCGTATATAGAGTCAGGGCTAGTTGTGGTGGCTCCAGTTAGGGGTTCAAATTCCGCTTGGACATTCTTAAAGCCATTTTCTTCATTTATGTGGGGTGTCACAGCATTTTTCTTCCTAGTCATTGGATTGGTGATATGGATTCTTGAACATAGAAAAAACGATGAGTTCCGAGGCCCTCCTAGGAAACAGGTTGTCACAATTTTATGGTTTAGCTTCTCTACCATGTTTTTTGCTCATAGAGAAAACATCGGGAGCACACTAGGCCGCACGGTGCTTATCATCTGGCTTTTCGTAGTTCTAGTGATCAACTCAAGCTACACAGCTAGCCTGACATCAATGCTCACAGTACAACAATTATCATCCCCCATCACCGGCATCGATACCTTGATAACTAGCACAGCACCTATAGGATTCCAAATCGGATCTTTTGCTCAGAGCTATTTGGTTGAGCAGCTCAACATCCCAAAGTCTAGACTAGTTCCTCTCGGCTCGCCAGAAGCTTATGCAACCGCGCTTAAGGACAAGACTGTTGCTGCTGTGGTTGATGAAAGAGCATACATAGAACTCTTCCTCTCAGAAAATTGCGAGTTCTCAATTAGGGGGTCGGAGTTCACCAAAAGTGGGTGGGGATTTGCATTTCCAAGAGACTCTCCTTTGGCAATTGACATGTCAACTGCCATACTCACTCTCTCTGAGAATGGTGATCTCCAGAGAATTTATAACAAATGGCTCTCCAGAAAGATTTGTGCTTCAGAGACCTCCGACGTCGTATCCGACCAGCTTCAACTACAAAGCTTTGGGGGGCTATTCCTTATTGCCGGCGCTGTATGTTTTATTGCTCTACTCATTCACTTGTTCTCCGTGTTACGCCAGTTCAAACGCCATACCCCCGAAGCTGAAGATCATTCTGAGCCTTCGAGCCACGGAAACATCAGCTCTCGTTCTACCTTAACACATCTCCTCACGTTCTTGTCCTTCGTCGACGAAAAGAGAGACGAATCGAAGAGAAATAAGTCGAAAAGGAAACGCAACGAAAGCGTGGTACCAAATGTGAATGAAAAGGAAGATGATCAATCGGTAAGGAGTGCTTCCAAGAGAATACAAATCAACAACTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

926

Amino Acids

101.63

Weight (kDa)

8.81

Isoelectric Point (pI)

40.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	36 - 325	6.9e-18	Periplasmic binding protein
ANF_receptor	PF01094	44 - 404	1.5e-84	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	478 - 564	3.2e-09	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	518 - 798	6.4e-11	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	583 - 829	5.8e-36	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003979)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17260 AT4G35290 AT4G35290 AT4G35290 AT4G35290 AT4G35290 AT4G35290
fragaria_vesca	FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810 FvH4_2g16810
malus_domestica	MD05G1139700.v1.1 MD10G1141900.v1.1
prunus_persica	Prupe.8G180400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280931
rosa_laevigata	RLG00000013015
rosa_multiflora	Rmu_ssc0000288.1_g000001
rosa_roxburghii	Rroxscaffold_7G00187480
rosa_rugosa	Rorug06G0136800 Rorug06G0136900 Rorug06G0137000 Rorug06G0137000 Rorug06G0137000
rosa_samantha	Rh6AG249300 Rh6CG252000 Rh6DG243800
rosa_wichuraiana	Rw6G005260 Rw6G021570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2379
AasI	GACNNNNNNGTC	1 cut(s)	344
AatII	GACGTC	1 cut(s)	2427
Acc36I	ACCTGC	1 cut(s)	79
Acc65I	GGTACC	1 cut(s)	2701
AccB1I	GGYRCC	1 cut(s)	2701
AccB7I	CCANNNNNTGG	3 cut(s)	762, 1309, 1373
AccI	GTMKAC	1 cut(s)	2640
AccII	CGCG	1 cut(s)	2171
AciI	CCGC	6 cut(s)	228, 417, 810, 1713, 1924, 2169
AclWI	GGATC	2 cut(s)	164, 2089
AcsI	RAATTY	3 cut(s)	298, 1708, 2373
AcuI	CTGAAG	3 cut(s)	1569, 2394, 2577
AcyI	GRCGYC	1 cut(s)	2424
AdeI	CACNNNGTG	1 cut(s)	539
AfaI	GTAC	7 cut(s)	260, 398, 1027, 1398, 1444, 2007, 2703
AfiI	CCNNNNNNNGG	8 cut(s)	762, 1040, 1294, 1309, 1373, 1700, 2030, 2280
AflII	CTTAAG	1 cut(s)	2174
AflIII	ACRYGT	2 cut(s)	343, 2325
AgeI	ACCGGT	2 cut(s)	1133, 1287
AhdI	GACNNNNNGTC	3 cut(s)	74, 840, 1426
AhlI	ACTAGT	2 cut(s)	1022, 2134
AjuI	GAANNNNNNNTTGG	2 cut(s)	1292, 1324
Alw21I	GWGCWC	3 cut(s)	253, 1569, 1916
Alw26I	GTCTC	6 cut(s)	660, 698, 1413, 2298, 2408, 2646
AlwI	GGATC	2 cut(s)	164, 2089
AlwNI	CAGNNNCTG	1 cut(s)	1105
Ama87I	CYCGRG	3 cut(s)	609, 657, 1350
AoxI	GGCC	4 cut(s)	1325, 1540, 1831, 1921
ApoI	RAATTY	3 cut(s)	298, 1708, 2373
AsiGI	ACCGGT	2 cut(s)	1133, 1287
Asp700I	GAANNNNTTC	1 cut(s)	1280
Asp718I	GGTACC	1 cut(s)	2701
AspA2I	CCTAGG	1 cut(s)	1838
AspLEI	GCGC	5 cut(s)	92, 370, 830, 2173, 2483
AspS9I	GGNCC	6 cut(s)	290, 373, 1031, 1145, 1326, 1832
AsuC2I	CCSGG	2 cut(s)	151, 1329
AsuHPI	GGTGA	9 cut(s)	490, 496, 551, 1547, 1622, 1801, 2020, 2263, 2372
AsuII	TTCGAA	1 cut(s)	643
AsuNHI	GCTAGC	2 cut(s)	812, 1982
AvaI	CYCGRG	3 cut(s)	609, 657, 1350
AvaII	GGWCC	4 cut(s)	290, 373, 1031, 1145
AvrII	CCTAGG	1 cut(s)	1838
BaeGI	GKGCMC	1 cut(s)	1269
BaeI	ACNNNNGTAYC	2 cut(s)	1434, 1467
BanI	GGYRCC	1 cut(s)	2701
BanII	GRGCYC	1 cut(s)	1569
Bbv12I	GWGCWC	3 cut(s)	253, 1569, 1916
BccI	CCATC	7 cut(s)	301, 433, 643, 724, 827, 1532, 2033
BceAI	ACGGC	3 cut(s)	381, 1159, 1645
BciVI	GTATCC	3 cut(s)	1226, 1454, 2440
BclI	TGATCA	3 cut(s)	427, 1962, 2728
BcnI	CCSGG	2 cut(s)	151, 1329
BcoDI	GTCTC	6 cut(s)	660, 698, 1413, 2298, 2408, 2646
BcuI	ACTAGT	2 cut(s)	1022, 2134
BfmI	CTRYAG	4 cut(s)	223, 392, 972, 2064
BfoI	RGCGCY	3 cut(s)	371, 831, 2484
BfrI	CTTAAG	1 cut(s)	2174
BfuAI	ACCTGC	1 cut(s)	79
BfuI	GTATCC	3 cut(s)	1226, 1454, 2440
BglI	GCCNNNNNGGC	1 cut(s)	143
BlnI	CCTAGG	1 cut(s)	1838
Bme1390I	CCNGG	2 cut(s)	151, 1329
Bme18I	GGWCC	4 cut(s)	290, 373, 1031, 1145
BmeRI	GACNNNNNGTC	3 cut(s)	74, 840, 1426
BmeT110I	CYCGRG	3 cut(s)	609, 657, 1350
BmgT120I	GGNCC	6 cut(s)	290, 373, 1031, 1145, 1326, 1832
BmiI	GGNNCC	4 cut(s)	49, 375, 1692, 2703
BmrFI	CCNGG	2 cut(s)	151, 1329
BmsI	GCATC	4 cut(s)	120, 948, 1588, 2043
BmtI	GCTAGC	2 cut(s)	816, 1986
BpmI	CTGGAG	3 cut(s)	1677, 2351, 2377
Bpu14I	TTCGAA	1 cut(s)	643
BpuEI	CTTGAG	3 cut(s)	49, 1529, 1954
BpuMI	CCSGG	2 cut(s)	151, 1329
Bsa29I	ATCGAT	1 cut(s)	2037
BsaAI	YACGTR	1 cut(s)	344
BsaHI	GRCGYC	1 cut(s)	2424
BsaI	GGTCTC	2 cut(s)	698, 2408
BsaJI	CCNNGG	5 cut(s)	823, 1349, 1828, 1838, 2581
BsaWI	WCCGGW	3 cut(s)	1133, 1147, 1287
BsaXI	ACNNNNNCTCC	4 cut(s)	665, 695, 2501, 2531
Bsc4I	CCNNNNNNNGG	8 cut(s)	762, 1040, 1294, 1309, 1373, 1700, 2030, 2280
Bse118I	RCCGGY	4 cut(s)	1133, 1287, 2030, 2477
Bse1I	ACTGG	5 cut(s)	1147, 1234, 1241, 1694, 2530
Bse3DI	GCAATG	4 cut(s)	99, 1002, 1479, 1614
BseCI	ATCGAT	1 cut(s)	2037
BseDI	CCNNGG	5 cut(s)	823, 1349, 1828, 1838, 2581
BseGI	GGATG	7 cut(s)	622, 732, 819, 963, 1159, 2018, 2118
BseLI	CCNNNNNNNGG	8 cut(s)	762, 1040, 1294, 1309, 1373, 1700, 2030, 2280
BseMI	GCAATG	4 cut(s)	99, 1002, 1479, 1614
BseMII	CTCAG	6 cut(s)	1263, 1670, 2105, 2247, 2343, 2559
BseNI	ACTGG	5 cut(s)	1147, 1234, 1241, 1694, 2530
BseRI	GAGGAG	2 cut(s)	1237, 2609
BseSI	GKGCMC	1 cut(s)	1269
BsgI	GTGCAG	1 cut(s)	516
Bsh1236I	CGCG	1 cut(s)	2171
Bsh1285I	CGRYCG	1 cut(s)	1623
BshFI	GGCC	4 cut(s)	1327, 1542, 1833, 1923
BshNI	GGYRCC	1 cut(s)	2701
BshTI	ACCGGT	2 cut(s)	1133, 1287
BshVI	ATCGAT	1 cut(s)	2037
BsiEI	CGRYCG	1 cut(s)	1623
BsiHKAI	GWGCWC	3 cut(s)	253, 1569, 1916
BsiHKCI	CYCGRG	3 cut(s)	609, 657, 1350
BsiSI	CCGG	8 cut(s)	150, 854, 1134, 1148, 1288, 1329, 2031, 2478
BslFI	GGGAC	3 cut(s)	332, 1069, 1348
BslI	CCNNNNNNNGG	8 cut(s)	762, 1040, 1294, 1309, 1373, 1700, 2030, 2280
BsmAI	GTCTC	6 cut(s)	660, 698, 1413, 2298, 2408, 2646
BsmBI	CGTCTC	1 cut(s)	2646
BsmFI	GGGAC	3 cut(s)	332, 1069, 1348
BsmI	GAATGC	2 cut(s)	169, 177
BsnI	GGCC	4 cut(s)	1327, 1542, 1833, 1923
Bso31I	GGTCTC	2 cut(s)	698, 2408
BsoBI	CYCGRG	3 cut(s)	609, 657, 1350
Bsp119I	TTCGAA	1 cut(s)	643
Bsp1286I	GDGCHC	4 cut(s)	253, 1269, 1569, 1916
Bsp143I	GATC	8 cut(s)	169, 427, 1620, 1962, 2081, 2362, 2560, 2728
BspACI	CCGC	6 cut(s)	228, 417, 810, 1713, 1924, 2169
BspANI	GGCC	4 cut(s)	1327, 1542, 1833, 1923
BspCNI	CTCAG	6 cut(s)	1264, 1669, 2104, 2246, 2344, 2560
BspDI	ATCGAT	1 cut(s)	2037
BspFNI	CGCG	1 cut(s)	2171
BspHI	TCATGA	1 cut(s)	211
BspLI	GGNNCC	4 cut(s)	49, 375, 1692, 2703
BspMAI	CTGCAG	1 cut(s)	396
BspMI	ACCTGC	1 cut(s)	79
BspOI	GCTAGC	2 cut(s)	816, 1986
BspPI	GGATC	2 cut(s)	164, 2089
BspT104I	TTCGAA	1 cut(s)	643
BspT107I	GGYRCC	1 cut(s)	2701
BspTI	CTTAAG	1 cut(s)	2174
BspTNI	GGTCTC	2 cut(s)	698, 2408
BsrDI	GCAATG	4 cut(s)	99, 1002, 1479, 1614
BsrFI	RCCGGY	4 cut(s)	1133, 1287, 2030, 2477
BsrI	ACTGG	5 cut(s)	1147, 1234, 1241, 1694, 2530
BssAI	RCCGGY	4 cut(s)	1133, 1287, 2030, 2477
BssECI	CCNNGG	5 cut(s)	823, 1349, 1828, 1838, 2581
BssMI	GATC	8 cut(s)	169, 427, 1620, 1962, 2081, 2362, 2560, 2728
BssNI	GRCGYC	1 cut(s)	2424
BssT1I	CCWWGG	2 cut(s)	823, 1838
Bst4CI	ACNGT	9 cut(s)	357, 775, 952, 1318, 1342, 1645, 1930, 2005, 2188
Bst6I	CTCTTC	3 cut(s)	843, 2231, 2657
BstACI	GRCGYC	1 cut(s)	2424
BstAFI	CTTAAG	1 cut(s)	2174
BstAPI	GCANNNNNTGC	1 cut(s)	329
BstBAI	YACGTR	1 cut(s)	344
BstBI	TTCGAA	1 cut(s)	643
BstC8I	GCNNGC	6 cut(s)	106, 814, 963, 1984, 2150, 2479
BstDEI	CTNAG	9 cut(s)	254, 861, 1272, 1293, 1656, 2091, 2233, 2352, 2568
BstDSI	CCRYGG	1 cut(s)	2581
BstF5I	GGATG	7 cut(s)	622, 732, 819, 963, 1159, 2018, 2118
BstFNI	CGCG	1 cut(s)	2171
BstH2I	RGCGCY	3 cut(s)	371, 831, 2484
BstHHI	GCGC	5 cut(s)	92, 370, 830, 2173, 2483
BstKTI	GATC	8 cut(s)	172, 430, 1623, 1965, 2084, 2365, 2563, 2731
BstMAI	GTCTC	6 cut(s)	660, 698, 1413, 2298, 2408, 2646
BstMBI	GATC	8 cut(s)	169, 427, 1620, 1962, 2081, 2362, 2560, 2728
BstMCI	CGRYCG	1 cut(s)	1623
BstMWI	GCNNNNNNNGC	8 cut(s)	45, 98, 143, 183, 329, 449, 1920, 2694
BstNSI	RCATGY	1 cut(s)	2329
BstSCI	CCNGG	2 cut(s)	149, 1327
BstSFI	CTRYAG	4 cut(s)	223, 392, 972, 2064
BstSLI	GKGCMC	1 cut(s)	1269
BstUI	CGCG	1 cut(s)	2171
BstX2I	RGATCY	1 cut(s)	2081
BstXI	CCANNNNNNTGG	1 cut(s)	778
BstYI	RGATCY	1 cut(s)	2081
Bsu15I	ATCGAT	1 cut(s)	2037
BsuI	GTATCC	3 cut(s)	1226, 1454, 2440
BsuRI	GGCC	4 cut(s)	1327, 1542, 1833, 1923
BsuTUI	ATCGAT	1 cut(s)	2037
BtgI	CCRYGG	1 cut(s)	2581
BtsCI	GGATG	7 cut(s)	622, 732, 819, 963, 1159, 2018, 2118
BtsI	GCAGTG	1 cut(s)	588
BtsIMutI	CAGTG	3 cut(s)	588, 771, 1314
BveI	ACCTGC	1 cut(s)	79
Cac8I	GCNNGC	6 cut(s)	106, 814, 963, 1984, 2150, 2479
CaiI	CAGNNNCTG	1 cut(s)	1105
CciI	TCATGA	1 cut(s)	211
CfoI	GCGC	5 cut(s)	92, 370, 830, 2173, 2483
Cfr10I	RCCGGY	4 cut(s)	1133, 1287, 2030, 2477
Cfr13I	GGNCC	6 cut(s)	290, 373, 1031, 1145, 1326, 1832
ClaI	ATCGAT	1 cut(s)	2037
Csp6I	GTAC	7 cut(s)	259, 397, 1026, 1397, 1443, 2006, 2702
CspAI	ACCGGT	2 cut(s)	1133, 1287
CspCI	CAANNNNNGTGG	2 cut(s)	1626, 1661
CviAII	CATG	6 cut(s)	212, 262, 968, 1576, 1882, 2326
CviQI	GTAC	7 cut(s)	259, 397, 1026, 1397, 1443, 2006, 2702
DdeI	CTNAG	9 cut(s)	254, 861, 1272, 1293, 1656, 2091, 2233, 2352, 2568
DpnI	GATC	8 cut(s)	171, 429, 1622, 1964, 2083, 2364, 2562, 2730
DpnII	GATC	8 cut(s)	169, 427, 1620, 1962, 2081, 2362, 2560, 2728
DraIII	CACNNNGTG	1 cut(s)	539
DrdI	GACNNNNNNGTC	1 cut(s)	344
DriI	GACNNNNNGTC	3 cut(s)	74, 840, 1426
DseDI	GACNNNNNNGTC	1 cut(s)	344
Eam1104I	CTCTTC	3 cut(s)	843, 2231, 2657
Eam1105I	GACNNNNNGTC	3 cut(s)	74, 840, 1426
EarI	CTCTTC	3 cut(s)	843, 2231, 2657
Ecl136II	GAGCTC	1 cut(s)	1567
Eco130I	CCWWGG	2 cut(s)	823, 1838
Eco24I	GRGCYC	1 cut(s)	1569
Eco31I	GGTCTC	2 cut(s)	698, 2408
Eco32I	GATATC	1 cut(s)	1220
Eco47I	GGWCC	4 cut(s)	290, 373, 1031, 1145
Eco53kI	GAGCTC	1 cut(s)	1567
Eco57I	CTGAAG	3 cut(s)	1569, 2394, 2577
Eco88I	CYCGRG	3 cut(s)	609, 657, 1350
EcoICRI	GAGCTC	1 cut(s)	1567
EcoO109I	RGGNCCY	2 cut(s)	1031, 1832
EcoRV	GATATC	1 cut(s)	1220
EcoT14I	CCWWGG	2 cut(s)	823, 1838
EcoT38I	GRGCYC	1 cut(s)	1569
ErhI	CCWWGG	2 cut(s)	823, 1838
Esp3I	CGTCTC	1 cut(s)	2646
FaeI	CATG	6 cut(s)	215, 265, 971, 1579, 1885, 2329
FalI	AAGNNNNNCTT	2 cut(s)	2731, 2763
FaqI	GGGAC	3 cut(s)	332, 1069, 1348
FatI	CATG	6 cut(s)	211, 261, 967, 1575, 1881, 2325
FauI	CCCGC	1 cut(s)	817
FbaI	TGATCA	3 cut(s)	427, 1962, 2728
FblI	GTMKAC	1 cut(s)	2640
FokI	GGATG	7 cut(s)	629, 739, 806, 970, 1146, 2005, 2105
FriOI	GRGCYC	1 cut(s)	1569
GlaI	GCGC	5 cut(s)	91, 369, 829, 2172, 2482
GsuI	CTGGAG	3 cut(s)	1677, 2351, 2377
HaeII	RGCGCY	3 cut(s)	371, 831, 2484
HaeIII	GGCC	4 cut(s)	1327, 1542, 1833, 1923
HapII	CCGG	8 cut(s)	150, 854, 1134, 1148, 1288, 1329, 2031, 2478
HhaI	GCGC	5 cut(s)	92, 370, 830, 2173, 2483
Hin1I	GRCGYC	1 cut(s)	2424
Hin1II	CATG	6 cut(s)	215, 265, 971, 1579, 1885, 2329
Hin6I	GCGC	5 cut(s)	90, 368, 828, 2171, 2481
HinP1I	GCGC	5 cut(s)	90, 368, 828, 2171, 2481
HincII	GTYRAC	2 cut(s)	2331, 2641
HindII	GTYRAC	2 cut(s)	2331, 2641
HindIII	AAGCTT	2 cut(s)	2157, 2452
HpaII	CCGG	8 cut(s)	150, 854, 1134, 1148, 1288, 1329, 2031, 2478
HphI	GGTGA	9 cut(s)	490, 496, 551, 1547, 1622, 1801, 2020, 2263, 2372
Hpy166II	GTNNAC	7 cut(s)	293, 771, 845, 1573, 2271, 2331, 2641
Hpy8I	GTNNAC	7 cut(s)	293, 771, 845, 1573, 2271, 2331, 2641
Hpy99I	CGWCG	4 cut(s)	2426, 2429, 2642, 2645
HpyAV	CCTTC	4 cut(s)	1194, 1451, 2583, 2644
HpyCH4III	ACNGT	9 cut(s)	357, 775, 952, 1318, 1342, 1645, 1930, 2005, 2188
HpyCH4IV	ACGT	3 cut(s)	343, 2424, 2624
HpyF10VI	GCNNNNNNNGC	8 cut(s)	45, 98, 143, 183, 329, 449, 1920, 2694
HpyF3I	CTNAG	9 cut(s)	254, 861, 1272, 1293, 1656, 2091, 2233, 2352, 2568
HpySE526I	ACGT	3 cut(s)	343, 2424, 2624
Hsp92I	GRCGYC	1 cut(s)	2424
Hsp92II	CATG	6 cut(s)	215, 265, 971, 1579, 1885, 2329
HspAI	GCGC	5 cut(s)	90, 368, 828, 2171, 2481
KpnI	GGTACC	1 cut(s)	2705
KroI	GCCGGC	1 cut(s)	2477
KroNI	GCCGGC	1 cut(s)	2479
Ksp22I	TGATCA	3 cut(s)	427, 1962, 2728
Kzo9I	GATC	8 cut(s)	169, 427, 1620, 1962, 2081, 2362, 2560, 2728
LmnI	GCTCC	5 cut(s)	53, 248, 821, 1696, 1911
LweI	GCATC	4 cut(s)	120, 948, 1588, 2043
MaeII	ACGT	3 cut(s)	343, 2424, 2624
MaeIII	GTNAC	9 cut(s)	361, 539, 1428, 1556, 1610, 1624, 1756, 1852, 2523
MalI	GATC	8 cut(s)	171, 429, 1622, 1964, 2083, 2364, 2562, 2730
MboI	GATC	8 cut(s)	169, 427, 1620, 1962, 2081, 2362, 2560, 2728
MboII	GAAGA	8 cut(s)	792, 830, 1731, 1762, 2218, 2570, 2674, 2735
MfeI	CAATTG	1 cut(s)	2319
MflI	RGATCY	1 cut(s)	2081
MhlI	GDGCHC	4 cut(s)	253, 1269, 1569, 1916
MlyI	GAGTC	7 cut(s)	234, 275, 662, 829, 1416, 1679, 2300
MmeI	TCCRAC	5 cut(s)	243, 1038, 2244, 2445, 2457
MroNI	GCCGGC	1 cut(s)	2477
MroXI	GAANNNNTTC	1 cut(s)	1280
MseI	TTAA	8 cut(s)	128, 1047, 1200, 1344, 1727, 2175, 2609, 2776
MslI	CAYNNNNRTG	1 cut(s)	1747
MspA1I	CMGCKG	2 cut(s)	104, 228
MspCI	CTTAAG	1 cut(s)	2174
MspI	CCGG	8 cut(s)	150, 854, 1134, 1148, 1288, 1329, 2031, 2478
MspR9I	CCNGG	2 cut(s)	151, 1329
MunI	CAATTG	1 cut(s)	2319
Mva1269I	GAATGC	2 cut(s)	169, 177
MvnI	CGCG	1 cut(s)	2171
MwoI	GCNNNNNNNGC	8 cut(s)	45, 98, 143, 183, 329, 449, 1920, 2694
NaeI	GCCGGC	1 cut(s)	2479
NciI	CCSGG	2 cut(s)	151, 1329
NdeII	GATC	8 cut(s)	169, 427, 1620, 1962, 2081, 2362, 2560, 2728
NgoMIV	GCCGGC	1 cut(s)	2477
NheI	GCTAGC	2 cut(s)	812, 1982
NlaIII	CATG	6 cut(s)	215, 265, 971, 1579, 1885, 2329
NlaIV	GGNNCC	4 cut(s)	49, 375, 1692, 2703
NmeAIII	GCCGAG	1 cut(s)	2124
NmuCI	GTSAC	7 cut(s)	361, 539, 1428, 1610, 1624, 1756, 1852
NspI	RCATGY	1 cut(s)	2329
NspV	TTCGAA	1 cut(s)	643
PaeR7I	CTCGAG	2 cut(s)	609, 657
PagI	TCATGA	1 cut(s)	211
PciI	ACATGT	1 cut(s)	2325
PcsI	WCGNNNNNNNCGW	1 cut(s)	2430
PctI	GAATGC	2 cut(s)	169, 177
PdiI	GCCGGC	1 cut(s)	2479
PdmI	GAANNNNTTC	1 cut(s)	1280
PflMI	CCANNNNNTGG	3 cut(s)	762, 1309, 1373
PinAI	ACCGGT	2 cut(s)	1133, 1287
Ple19I	CGATCG	1 cut(s)	1623
PleI	GAGTC	7 cut(s)	234, 274, 661, 829, 1415, 1678, 2300
PpsI	GAGTC	7 cut(s)	234, 274, 661, 829, 1415, 1678, 2300
Ppu21I	YACGTR	1 cut(s)	344
PpuMI	RGGWCCY	1 cut(s)	1031
PscI	ACATGT	1 cut(s)	2325
PsiI	TTATAA	1 cut(s)	2379
Psp124BI	GAGCTC	1 cut(s)	1569
Psp5II	RGGWCCY	1 cut(s)	1031
PspN4I	GGNNCC	4 cut(s)	49, 375, 1692, 2703
PspPI	GGNCC	6 cut(s)	290, 373, 1031, 1145, 1326, 1832
PspPPI	RGGWCCY	1 cut(s)	1031
PsrI	GAACNNNNNNTAC	4 cut(s)	1426, 1458, 1541, 1573
PstI	CTGCAG	1 cut(s)	396
PstNI	CAGNNNCTG	1 cut(s)	1105
PsuI	RGATCY	1 cut(s)	2081
PvuI	CGATCG	1 cut(s)	1623
PvuII	CAGCTG	1 cut(s)	104
RsaI	GTAC	7 cut(s)	260, 398, 1027, 1398, 1444, 2007, 2703
RsaNI	GTAC	7 cut(s)	259, 397, 1026, 1397, 1443, 2006, 2702
RseI	CAYNNNNRTG	1 cut(s)	1747
SacI	GAGCTC	1 cut(s)	1569
SalI	GTCGAC	1 cut(s)	2639
SaqAI	TTAA	8 cut(s)	128, 1047, 1200, 1344, 1727, 2175, 2609, 2776
Sau3AI	GATC	8 cut(s)	169, 427, 1620, 1962, 2081, 2362, 2560, 2728
Sau96I	GGNCC	6 cut(s)	290, 373, 1031, 1145, 1326, 1832
SchI	GAGTC	7 cut(s)	234, 275, 662, 829, 1416, 1679, 2300
ScrFI	CCNGG	2 cut(s)	151, 1329
SduI	GDGCHC	4 cut(s)	253, 1269, 1569, 1916
SfaNI	GCATC	4 cut(s)	120, 948, 1588, 2043
SfcI	CTRYAG	4 cut(s)	223, 392, 972, 2064
Sfr274I	CTCGAG	2 cut(s)	609, 657
SfuI	TTCGAA	1 cut(s)	643
SgrDI	CGTCGACG	1 cut(s)	2639
SinI	GGWCC	4 cut(s)	290, 373, 1031, 1145
SlaI	CTCGAG	2 cut(s)	609, 657
SmiMI	CAYNNNNRTG	1 cut(s)	1747
SmlI	CTYRAG	6 cut(s)	64, 609, 657, 1544, 1969, 2174
SmoI	CTYRAG	6 cut(s)	64, 609, 657, 1544, 1969, 2174
SpeI	ACTAGT	2 cut(s)	1022, 2134
SsiI	CCGC	6 cut(s)	228, 417, 810, 1713, 1924, 2169
SspI	AATATT	1 cut(s)	1114
SstI	GAGCTC	1 cut(s)	1569
StyD4I	CCNGG	2 cut(s)	149, 1327
StyI	CCWWGG	2 cut(s)	823, 1838
TaaI	ACNGT	9 cut(s)	357, 775, 952, 1318, 1342, 1645, 1930, 2005, 2188
TaiI	ACGT	3 cut(s)	346, 2427, 2627
TatI	WGTACW	2 cut(s)	1025, 2005
TauI	GCSGC	1 cut(s)	1926
Tru1I	TTAA	8 cut(s)	128, 1047, 1200, 1344, 1727, 2175, 2609, 2776
Tru9I	TTAA	8 cut(s)	128, 1047, 1200, 1344, 1727, 2175, 2609, 2776
TscAI	CASTG	3 cut(s)	588, 778, 1321
TseFI	GTSAC	7 cut(s)	361, 539, 1428, 1610, 1624, 1756, 1852
Tsp45I	GTSAC	7 cut(s)	361, 539, 1428, 1610, 1624, 1756, 1852
TspGWI	ACGGA	3 cut(s)	132, 2509, 2598
TspRI	CASTG	3 cut(s)	588, 778, 1321
Van91I	CCANNNNNTGG	3 cut(s)	762, 1309, 1373
Vha464I	CTTAAG	1 cut(s)	2174
VpaK11BI	GGWCC	4 cut(s)	290, 373, 1031, 1145
XapI	RAATTY	3 cut(s)	298, 1708, 2373
XbaI	TCTAGA	1 cut(s)	2129
XceI	RCATGY	1 cut(s)	2329
XhoI	CTCGAG	2 cut(s)	609, 657
XmaJI	CCTAGG	1 cut(s)	1838
XmiI	GTMKAC	1 cut(s)	2640
XmnI	GAANNNNTTC	1 cut(s)	1280
ZraI	GACGTC	1 cut(s)	2425

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.