FvH4_2g19620
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013816
Physical Location & Seq
Forward (+)
16609950 .. 16613898
3949 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19620.t1

Sequence Viewer

Length: 3453 bp
ATGCCAATGCTGAGGAGGCAGCAGCCCTCTAACACCTACAATCACCAACAAATGTCGAGGCAACTATCGTTCACCACCATCGATTTCTTCTTCCTCTTCATCTTCACACTCGCAACGTCTGCTGCTTCTTTGGCTGCTGCAAATGTTGAGGTTATCACATTACATTCATGGCTTCAAGGCTCTACTACCTCGGCCTCTTCTTCGAATTTCTCCAACTGGAACCCTTCAGACCCCAACCCTTGTAAGTGGTCCTATATTACTTGTTCACCCCAAAATTTTGTTACTGAGATCAGTATCCAGTCTATTGAGCTAGCCATTCCCTTTCCTTCAAATTTTGCCTCCCTTGCATTTCTTCAAAAGCTCATCATTTCTGATGCTAATCTTACTGGAACAATCTCACCTGATATTGGAAATTGCAAATCACTTGTGGTGCTTGATGTACATTCAAACAGTCTTGTGGGTAGTATTCCTACAAGTATTGGGATGCTTGAAAATCTTCAGGACCTGATCTTAAACTCTAACCAGCTTACTGGTCAAGTACCAAATGAGATTGGTGATTGCAGCAGCCTGAAAAATCTTCTCTTGTTTGATAACTACCTAAGTGGTTATCTTCCTGCTACACTCGGAAAGTTACGAAACTTGGAAGCTATTCGAGCTGGAGGCAACAAAAACATGTCAGGAAAGATCCCAGATGAGCTTGGAAACTGCAAGAACTTGCAGGTGTTGGGGCTTGCTGAAACGAACATTTCTGGTTCGATACCTTCATCATTAGGTAAGCTCAGCATGCTGCAAACGCTGTCGATTTATACTACAATGATATCCGGTAAGATTCCTCCGGATATTGGCAACTGTTCAGAACTTGTCGACTTGTTCTTGTATGAGAATTACCTTTCTGGTTCACTACCACCAGAGTTGGGCAAGCTTCATAAGCTCGAGAAGATGTTATTGTGGCAAAACAACCTTGATGGGACTATTCCTGAGGAGATTGGGAATTGCAAAAGTTTGAAGACCATAGATCTCTCTCTAAACTCTGTTTCAGGAAGCATACCACAGTCTTTTGGAAACCTCTCAAATCTTGAAGACCTTATGCTTAGTAACAACAACATCTCTGGTTCAATCCCCTCAGTTCTTTCCACTGCAACAAAACTGGTGCAGCTGCAGGTTGATGCTAATCAGATTTCAGGCTTGATCCCAGCTGAGCTGGGAATGTTGAAGGAGCTCATAGTCTTTTTCGCTTGGCAGAACAAACTCGAAGGCAGCATCCCATCGGAAATGGCTGGTTGTGAGAGCCTTGAAGCTCTTGATCTCTCACACAATGCTCTCACTGGTAACATACCTTCTGGCCTATTTCAGCTGCAAAATTTAACCAAGCTTCTCTTGATTTCTAATGACATTTCGGGTCCTATACCTTCGGTGATTGGAAATTGTACCTCTCTTAACCGGCTAAGGCTTGTGCATAACAGAATCAGTGGAGAGATCCCAAAAGAAATTGGGTTCCTAGATAAACTTGGTTTCCTTGATCTGTCTGAAAACCGTCTTGTTGGTACTGTACCAGAAGAAATTGGAAAATGCAGTTCATTACAGCTACTCAACTTAAGTAACAACACTCTTAGAGGTAATTTGCCTGGCTCTTTGTCTTCTCTCACAGAGCTTGAAGTATTGGATGTCTCTGTGAATCAATTTGGAGGTCAGATTCCTGAGAGTTACGGTGGACTAGCTTCACTTAACAGGCTCATCCTCAGCAGCAACTCCTTAACTGATCCAATTCCCTCTTCACTTGGTAAATGTTCAAAACTTCAACTGCTTGATCTTAGTAACAACAAGCTCACTGGGATGATCCCGGAGCAACTGTTTGAAATTGAAGCTCTTGGCATTGCTTTGAACCTGAGTTTCAATGCTTTATATGGCATAATCCCACCACAGATTTCTGCTCTCAATAAGCTATCCATATTAGACCTATCACACAACAAGCTTGAAGGTGATCTGTTGGCACTTTCGGGGCTTGAAAACCTTGTTTCTTTGAACATCTCATACAACAACTTCACTGGTTATCTTCCCGACAGCAAAGTGTTTCGACAGTTGTCTGCGACAGACTTGGTGGGAAACAACGGATTGTGCTCTAGAGGTCATGATTTATGTTTTCTCAGCGATGGTACAACAATGGCTTTGTCTAGAAGTGGAAGTTTTAGGAGGTCATGGAGGCTTAAATTGGCCATTGCTTTGCTAATAGCCTTGACCATTGCTTTGTCAGTATTTGGGGTAGTTGCAGTATTTCGAGCACGAAAGATGAAAGGGGAGGACAATGATTCTGAGATGGGAGGGGACTCATGGCCTTGGCAATTCACTCCATTTCAGAAGGTGCGTTTCTCAGTTGAGCAAGTTCTGAAATGTCTTGTAGAAACCAATGTAATAGGAAAGGGATGCTCAGGAGTTGTTTACCGGGCAGAAATGGAAACCGAAGACATTGCAGTAAAGAAGCTCTGGCCAACAACAACTGCTTCCAAATATGATTCTCAAAGTAGAGGAGTTCGTGATTCCTTTTCAACTGAGGTGAAGACCCTTGGCTCCATTCGCCACAAGAACATTGTTAGGTTCTTAGGTTGCTGTTGGAACCGAAACACAAGACTGCTAATGTATGAGTACATGCCGAATGGGAGTTTGGGAGGTCTACTTCATGAAAGAAGAGGTAACTGCTTGGAATGGGACATCAGATATCGCATAATACTCGGGGCAGCACAAGGCTTGGCATATTTGCACCATGACTGTGTTCCTCCTATTGTTCACAGGGACATCAAGGCCAACAACATTCTCATTGGTCCAGATTTTGAGCCCTGCATTGCTGATTTTGGGCTTGCCAAGCTTGTTGATGAAGGAGATTTTGCTCGGTCCTCCAATACAATTGCTGGTTCTTATGGCTACATTGCTCCTGAATATGGATACTCAATGAAGATAACAGAGAAAAGCGATGTGTACAGCTACGGTGTAGTTGTGCTAGAAGTGCTAACAGGAAAGCAACCAATTGATCCAACCATACCAGATGGACTCCACATTGTGGATTGGGTAAGGCAGAAGAGAGGAGCAGTTGAGGTACTTGATCAAAGCTTACGAGCACGACCTGAACCCGAGATTGAGGAAATGCTGCAGACATTAGGTGTGGCATTGCTGTGCATAAACCCAACCCCAGATGACAGACCAACCATGAAAGATGTAGCAGCAATGCTCAAAGAGATTAGGCAGGAGAGAGAGGAGTGCATGAAAGCTAACATGAGACTTCTCAATAAGTCCAATTCTGCAAATGATCATAAACAAGAAACTATGATCAGTTGCAGTGGAGGAACATCATCAGCTATGGTGCAGCAACAACATCCATACCCTCCAACAAGTACCAGCACAAGTTTCTCTGCTTCCTCACTGTTATACTCTTCATCTTCCAATGTCAAAACAACTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1151

Amino Acids

125.48

Weight (kDa)

5.53

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 118 - 218 2.2e-06 Leucine-rich repeat region
LRR_14 PF23598 231 - 317 7.9e-09 Leucine-rich repeat region
LRR_8 PF13855 310 - 369 6.7e-07 Leucine rich repeat
LRR_14 PF23598 324 - 410 1.1e-06 Leucine-rich repeat region
LRR_8 PF13855 334 - 393 3.1e-07 Leucine rich repeat
LRR_14 PF23598 482 - 699 9e-15 Leucine-rich repeat region
LRR_8 PF13855 506 - 561 2e-06 Leucine rich repeat
LRR_8 PF13855 574 - 622 3.7e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 801 - 1073 4e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 801 - 1071 1e-40 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48940
fragaria_vesca FvH4_2g19620
malus_domestica MD10G1169800.v1.1
prunus_persica Prupe.8G207600_v2.0.a1
pyrus_communis pycom10g14630
rosa_chinensis RchiOBHm_Chr6g0285351
rosa_laevigata RLG00000012651
rosa_multiflora Rmu_co8152086.1_g000001 Rmu_co8221716.1_g000001 Rmu_sc0012287.1_g000004
rosa_roxburghii Rroxscaffold_7G00183330
rosa_rugosa Rorug06G0173900
rosa_samantha Rh6AG284500 Rh6BG285700 Rh6CG286800 Rh6DG279800
rosa_wichuraiana Rw6G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 709
Acc36I ACCTGC 2 cut(s) 709, 1150
AccB7I CCANNNNNTGG 1 cut(s) 3052
AccI GTMKAC 2 cut(s) 864, 2668
AccIII TCCGGA 1 cut(s) 835
AclWI GGATC 6 cut(s) 679, 1183, 1471, 1754, 1831, 3017
AcoI YGGCCR 2 cut(s) 2211, 2483
AcsI RAATTY 4 cut(s) 205, 274, 331, 1360
AcuI CTGAAG 2 cut(s) 210, 482
AdeI CACNNNGTG 1 cut(s) 3052
AfiI CCNNNNNNNGG 5 cut(s) 407, 842, 914, 2933, 3052
AflII CTTAAG 1 cut(s) 1594
AflIII ACRYGT 1 cut(s) 672
AjnI CCWGG 1 cut(s) 1624
AjuI GAANNNNNNNTTGG 4 cut(s) 1756, 1788, 2642, 2674
Alw21I GWGCWC 4 cut(s) 1221, 2120, 2281, 3112
Alw26I GTCTC 2 cut(s) 1672, 3262
AlwI GGATC 6 cut(s) 679, 1183, 1471, 1754, 1831, 3017
AlwNI CAGNNNCTG 1 cut(s) 505
Ama87I CYCGRG 3 cut(s) 932, 2726, 3122
Aor13HI TCCGGA 1 cut(s) 835
AoxI GGCC 6 cut(s) 192, 1342, 2211, 2330, 2483, 2796
ApoI RAATTY 4 cut(s) 205, 274, 331, 1360
ArsI GACNNNNNNTTYG 2 cut(s) 2227, 2259
Asp700I GAANNNNTTC 2 cut(s) 495, 648
AspS9I GGNCC 5 cut(s) 249, 502, 1400, 2816, 2886
AsuC2I CCSGG 2 cut(s) 1841, 2441
AsuHPI GGTGA 8 cut(s) 35, 64, 258, 390, 566, 1426, 1991, 2563
AsuII TTCGAA 1 cut(s) 203
AsuNHI GCTAGC 1 cut(s) 310
AvaI CYCGRG 3 cut(s) 932, 2726, 3122
AvaII GGWCC 5 cut(s) 249, 502, 1400, 2816, 2886
AxyI CCTNAGG 1 cut(s) 978
BalI TGGCCA 2 cut(s) 2213, 2485
BanII GRGCYC 2 cut(s) 1221, 2832
BarI GAAGNNNNNNTAC 2 cut(s) 1321, 1353
BbsI GAAGAC 5 cut(s) 1013, 1086, 1629, 2466, 2561
Bbv12I GWGCWC 4 cut(s) 1221, 2120, 2281, 3112
BbvCI CCTCAGC 2 cut(s) 11, 1739
BccI CCATC 6 cut(s) 86, 959, 1273, 2144, 2308, 3032
BcgI CGANNNNNNTGC 4 cut(s) 2447, 2481, 2707, 2741
BciT130I CCWGG 1 cut(s) 1626
BciVI GTATCC 2 cut(s) 305, 2930
BclI TGATCA 3 cut(s) 3094, 3298, 3318
BcnI CCSGG 2 cut(s) 1841, 2441
BcoDI GTCTC 2 cut(s) 1672, 3262
BfaI CTAG 6 cut(s) 311, 1499, 1715, 2121, 2172, 2993
BfmI CTRYAG 2 cut(s) 1157, 3140
BfrI CTTAAG 1 cut(s) 1594
BfuAI ACCTGC 2 cut(s) 709, 1150
BfuI GTATCC 2 cut(s) 305, 2930
BglII AGATCT 1 cut(s) 1015
BlpI GCTNAGC 2 cut(s) 779, 1197
Bme1390I CCNGG 3 cut(s) 1626, 1841, 2441
Bme18I GGWCC 5 cut(s) 249, 502, 1400, 2816, 2886
BmeT110I CYCGRG 3 cut(s) 932, 2726, 3122
BmgT120I GGNCC 5 cut(s) 249, 502, 1400, 2816, 2886
BmiI GGNNCC 5 cut(s) 221, 1401, 1496, 2566, 2612
BmrFI CCNGG 3 cut(s) 1626, 1841, 2441
BmrI ACTGGG 1 cut(s) 1839
BmsI GCATC 5 cut(s) 364, 474, 1156, 1269, 2411
BmtI GCTAGC 1 cut(s) 314
BmuI ACTGGG 1 cut(s) 1839
BoxI GACNNNNGTC 1 cut(s) 2080
BpiI GAAGAC 5 cut(s) 1013, 1086, 1629, 2466, 2561
BpmI CTGGAG 1 cut(s) 678
Bpu10I CCTNAGC 4 cut(s) 11, 1445, 1739, 2425
Bpu1102I GCTNAGC 2 cut(s) 779, 1197
Bpu14I TTCGAA 1 cut(s) 203
BpuMI CCSGG 2 cut(s) 1841, 2441
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 3 cut(s) 293, 378, 1170
BsaJI CCNNGG 3 cut(s) 189, 2333, 2560
BsaWI WCCGGW 2 cut(s) 821, 835
BsaXI ACNNNNNCTCC 2 cut(s) 1835, 1865
Bsc4I CCNNNNNNNGG 5 cut(s) 407, 842, 914, 2933, 3052
Bse118I RCCGGY 1 cut(s) 1440
Bse1I ACTGG 8 cut(s) 221, 298, 391, 535, 1152, 1330, 1834, 2050
Bse21I CCTNAGG 1 cut(s) 978
Bse3DI GCAATG 8 cut(s) 1872, 2214, 2238, 2463, 2835, 2919, 3158, 3222
Bse8I GATNNNNATC 3 cut(s) 293, 378, 1170
BseAI TCCGGA 1 cut(s) 835
BseBI CCWGG 1 cut(s) 1626
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 3 cut(s) 189, 2333, 2560
BseGI GGATG 7 cut(s) 489, 1260, 1669, 1734, 1839, 2426, 3364
BseJI GATNNNNATC 3 cut(s) 293, 378, 1170
BseLI CCNNNNNNNGG 5 cut(s) 407, 842, 914, 2933, 3052
BseMI GCAATG 8 cut(s) 1872, 2214, 2238, 2463, 2835, 2919, 3158, 3222
BseNI ACTGG 8 cut(s) 221, 298, 391, 535, 1152, 1330, 1834, 2050
BseRI GAGGAG 5 cut(s) 28, 995, 2538, 3090, 3260
BseYI CCCAGC 2 cut(s) 1192, 1201
BsgI GTGCAG 2 cut(s) 1172, 3374
BshFI GGCC 6 cut(s) 194, 1344, 2213, 2332, 2485, 2798
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 4 cut(s) 1221, 2120, 2281, 3112
BsiHKCI CYCGRG 3 cut(s) 932, 2726, 3122
BsiSI CCGG 5 cut(s) 822, 836, 1441, 1841, 2440
BslFI GGGAC 4 cut(s) 982, 2336, 2717, 2801
BslI CCNNNNNNNGG 5 cut(s) 407, 842, 914, 2933, 3052
BsmAI GTCTC 2 cut(s) 1672, 3262
BsmFI GGGAC 4 cut(s) 982, 2336, 2717, 2801
BsnI GGCC 6 cut(s) 194, 1344, 2213, 2332, 2485, 2798
BsoBI CYCGRG 3 cut(s) 932, 2726, 3122
Bsp119I TTCGAA 1 cut(s) 203
Bsp1286I GDGCHC 5 cut(s) 1221, 2120, 2281, 2832, 3112
Bsp13I TCCGGA 1 cut(s) 835
Bsp1407I TGTACA 2 cut(s) 439, 2970
Bsp1720I GCTNAGC 2 cut(s) 779, 1197
BspANI GGCC 6 cut(s) 194, 1344, 2213, 2332, 2485, 2798
BspDI ATCGAT 1 cut(s) 81
BspEI TCCGGA 1 cut(s) 835
BspHI TCATGA 2 cut(s) 2128, 2674
BspLI GGNNCC 5 cut(s) 221, 1401, 1496, 2566, 2612
BspMAI CTGCAG 2 cut(s) 1161, 3144
BspMI ACCTGC 2 cut(s) 709, 1150
BspOI GCTAGC 1 cut(s) 314
BspPI GGATC 6 cut(s) 679, 1183, 1471, 1754, 1831, 3017
BspT104I TTCGAA 1 cut(s) 203
BspTI CTTAAG 1 cut(s) 1594
BsrDI GCAATG 8 cut(s) 1872, 2214, 2238, 2463, 2835, 2919, 3158, 3222
BsrFI RCCGGY 1 cut(s) 1440
BsrGI TGTACA 2 cut(s) 439, 2970
BsrI ACTGG 8 cut(s) 221, 298, 391, 535, 1152, 1330, 1834, 2050
BssAI RCCGGY 1 cut(s) 1440
BssECI CCNNGG 3 cut(s) 189, 2333, 2560
BssT1I CCWWGG 2 cut(s) 2333, 2560
Bst2UI CCWGG 1 cut(s) 1626
Bst6I CTCTTC 6 cut(s) 101, 202, 1777, 2677, 3065, 3427
BstAFI CTTAAG 1 cut(s) 1594
BstAPI GCANNNNNTGC 1 cut(s) 119
BstAUI TGTACA 2 cut(s) 439, 2970
BstBI TTCGAA 1 cut(s) 203
BstC8I GCNNGC 5 cut(s) 312, 732, 785, 920, 2853
BstF5I GGATG 7 cut(s) 489, 1260, 1669, 1734, 1839, 2426, 3364
BstMAI GTCTC 2 cut(s) 1672, 3262
BstNI CCWGG 1 cut(s) 1626
BstNSI RCATGY 3 cut(s) 676, 787, 2647
BstPAI GACNNNNGTC 1 cut(s) 2080
BstSCI CCNGG 3 cut(s) 1624, 1839, 2439
BstSFI CTRYAG 2 cut(s) 1157, 3140
BstV2I GAAGAC 5 cut(s) 1013, 1086, 1629, 2466, 2561
BstX2I RGATCY 3 cut(s) 684, 1015, 1476
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 3 cut(s) 684, 1015, 1476
Bsu15I ATCGAT 1 cut(s) 81
Bsu36I CCTNAGG 1 cut(s) 978
BsuI GTATCC 2 cut(s) 305, 2930
BsuRI GGCC 6 cut(s) 194, 1344, 2213, 2332, 2485, 2798
BsuTUI ATCGAT 1 cut(s) 81
BtgZI GCGATG 2 cut(s) 2163, 2979
BtsCI GGATG 7 cut(s) 489, 1260, 1669, 1734, 1839, 2426, 3364
BtsI GCAGTG 2 cut(s) 1134, 3334
BtsIMutI CAGTG 7 cut(s) 1134, 1323, 1474, 1827, 2043, 3334, 3410
BveI ACCTGC 2 cut(s) 709, 1150
Cac8I GCNNGC 5 cut(s) 312, 732, 785, 920, 2853
CaiI CAGNNNCTG 1 cut(s) 505
CciI TCATGA 2 cut(s) 2128, 2674
Cfr10I RCCGGY 1 cut(s) 1440
Cfr13I GGNCC 5 cut(s) 249, 502, 1400, 2816, 2886
ClaI ATCGAT 1 cut(s) 81
CspCI CAANNNNNGTGG 2 cut(s) 894, 929
DraIII CACNNNGTG 1 cut(s) 3052
EaeI YGGCCR 2 cut(s) 2211, 2483
Eam1104I CTCTTC 6 cut(s) 101, 202, 1777, 2677, 3065, 3427
EarI CTCTTC 6 cut(s) 101, 202, 1777, 2677, 3065, 3427
Ecl136II GAGCTC 1 cut(s) 1219
Eco130I CCWWGG 2 cut(s) 2333, 2560
Eco24I GRGCYC 2 cut(s) 1221, 2832
Eco32I GATATC 2 cut(s) 819, 2714
Eco47I GGWCC 5 cut(s) 249, 502, 1400, 2816, 2886
Eco53kI GAGCTC 1 cut(s) 1219
Eco57I CTGAAG 2 cut(s) 210, 482
Eco81I CCTNAGG 1 cut(s) 978
Eco88I CYCGRG 3 cut(s) 932, 2726, 3122
EcoICRI GAGCTC 1 cut(s) 1219
EcoO109I RGGNCCY 2 cut(s) 502, 1400
EcoRII CCWGG 1 cut(s) 1624
EcoRV GATATC 2 cut(s) 819, 2714
EcoT14I CCWWGG 2 cut(s) 2333, 2560
EcoT38I GRGCYC 2 cut(s) 1221, 2832
ErhI CCWWGG 2 cut(s) 2333, 2560
FalI AAGNNNNNCTT 2 cut(s) 1361, 1393
FaqI GGGAC 4 cut(s) 982, 2336, 2717, 2801
FbaI TGATCA 3 cut(s) 3094, 3298, 3318
FblI GTMKAC 2 cut(s) 864, 2668
FokI GGATG 7 cut(s) 496, 1247, 1676, 1721, 1846, 2433, 3351
FriOI GRGCYC 2 cut(s) 1221, 2832
FspBI CTAG 6 cut(s) 311, 1499, 1715, 2121, 2172, 2993
GsaI CCCAGC 2 cut(s) 1196, 1205
GsuI CTGGAG 1 cut(s) 678
HaeIII GGCC 6 cut(s) 194, 1344, 2213, 2332, 2485, 2798
HapII CCGG 5 cut(s) 822, 836, 1441, 1841, 2440
HincII GTYRAC 1 cut(s) 865
HindII GTYRAC 1 cut(s) 865
HindIII AAGCTT 5 cut(s) 920, 1370, 1970, 2858, 3100
HinfI GANTC 9 cut(s) 829, 1464, 1675, 1693, 2306, 2324, 2510, 2534, 3042
HpaII CCGG 5 cut(s) 822, 836, 1441, 1841, 2440
HphI GGTGA 8 cut(s) 35, 64, 258, 390, 566, 1426, 1991, 2563
Hpy166II GTNNAC 9 cut(s) 72, 266, 865, 899, 1712, 2437, 2669, 2782, 2971
Hpy8I GTNNAC 9 cut(s) 72, 266, 865, 899, 1712, 2437, 2669, 2782, 2971
HpyCH4IV ACGT 1 cut(s) 116
HpySE526I ACGT 1 cut(s) 116
Kpn2I TCCGGA 1 cut(s) 835
Ksp22I TGATCA 3 cut(s) 3094, 3298, 3318
LmnI GCTCC 5 cut(s) 1216, 1843, 2570, 2929, 3077
LweI GCATC 5 cut(s) 364, 474, 1156, 1269, 2411
MaeI CTAG 6 cut(s) 311, 1499, 1715, 2121, 2172, 2993
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 8 cut(s) 280, 630, 1094, 1328, 1598, 1703, 1814, 2687
MfeI CAATTG 2 cut(s) 2898, 3018
MflI RGATCY 3 cut(s) 684, 1015, 1476
MhlI GDGCHC 5 cut(s) 1221, 2120, 2281, 2832, 3112
MlsI TGGCCA 2 cut(s) 2213, 2485
MluNI TGGCCA 2 cut(s) 2213, 2485
MlyI GAGTC 2 cut(s) 2318, 3036
MmeI TCCRAC 4 cut(s) 237, 2588, 3050, 3401
Mox20I TGGCCA 2 cut(s) 2213, 2485
MroI TCCGGA 1 cut(s) 835
MroXI GAANNNNTTC 2 cut(s) 495, 648
MscI TGGCCA 2 cut(s) 2213, 2485
MseI TTAA 7 cut(s) 512, 1364, 1437, 1595, 1725, 1754, 2205
MslI CAYNNNNRTG 3 cut(s) 1832, 2763, 3163
Msp20I TGGCCA 2 cut(s) 2213, 2485
MspA1I CMGCKG 3 cut(s) 1156, 1196, 1354
MspCI CTTAAG 1 cut(s) 1594
MspI CCGG 5 cut(s) 822, 836, 1441, 1841, 2440
MspR9I CCNGG 3 cut(s) 1626, 1841, 2441
MunI CAATTG 2 cut(s) 2898, 3018
MvaI CCWGG 1 cut(s) 1626
NciI CCSGG 2 cut(s) 1841, 2441
NheI GCTAGC 1 cut(s) 310
NlaIV GGNNCC 5 cut(s) 221, 1401, 1496, 2566, 2612
NmeAIII GCCGAG 1 cut(s) 170
NspI RCATGY 3 cut(s) 676, 787, 2647
NspV TTCGAA 1 cut(s) 203
PaeI GCATGC 1 cut(s) 787
PaeR7I CTCGAG 1 cut(s) 932
PagI TCATGA 2 cut(s) 2128, 2674
PaqCI CACCTGC 1 cut(s) 709
PciI ACATGT 1 cut(s) 672
PdmI GAANNNNTTC 2 cut(s) 495, 648
PfeI GAWTC 7 cut(s) 829, 1464, 1675, 1693, 2306, 2510, 2534
PflMI CCANNNNNTGG 1 cut(s) 3052
PfoI TCCNGGA 1 cut(s) 1839
PleI GAGTC 2 cut(s) 2318, 3036
PpsI GAGTC 2 cut(s) 2318, 3036
PpuMI RGGWCCY 2 cut(s) 502, 1400
PscI ACATGT 1 cut(s) 672
PshAI GACNNNNGTC 1 cut(s) 2080
Psp124BI GAGCTC 1 cut(s) 1221
Psp5II RGGWCCY 2 cut(s) 502, 1400
Psp6I CCWGG 1 cut(s) 1624
PspFI CCCAGC 2 cut(s) 1192, 1201
PspGI CCWGG 1 cut(s) 1624
PspN4I GGNNCC 5 cut(s) 221, 1401, 1496, 2566, 2612
PspPI GGNCC 5 cut(s) 249, 502, 1400, 2816, 2886
PspPPI RGGWCCY 2 cut(s) 502, 1400
PsrI GAACNNNNNNTAC 2 cut(s) 2015, 2047
PstI CTGCAG 2 cut(s) 1161, 3144
PstNI CAGNNNCTG 1 cut(s) 505
PsuI RGATCY 3 cut(s) 684, 1015, 1476
PvuII CAGCTG 3 cut(s) 1156, 1196, 1354
RseI CAYNNNNRTG 3 cut(s) 1832, 2763, 3163
SacI GAGCTC 1 cut(s) 1221
SalI GTCGAC 1 cut(s) 863
SaqAI TTAA 7 cut(s) 512, 1364, 1437, 1595, 1725, 1754, 2205
Sau96I GGNCC 5 cut(s) 249, 502, 1400, 2816, 2886
SchI GAGTC 2 cut(s) 2318, 3036
ScrFI CCNGG 3 cut(s) 1626, 1841, 2441
SduI GDGCHC 5 cut(s) 1221, 2120, 2281, 2832, 3112
SfaNI GCATC 5 cut(s) 364, 474, 1156, 1269, 2411
SfcI CTRYAG 2 cut(s) 1157, 3140
Sfr274I CTCGAG 1 cut(s) 932
SfuI TTCGAA 1 cut(s) 203
SinI GGWCC 5 cut(s) 249, 502, 1400, 2816, 2886
SlaI CTCGAG 1 cut(s) 932
SmiMI CAYNNNNRTG 3 cut(s) 1832, 2763, 3163
SmlI CTYRAG 2 cut(s) 932, 1594
SmoI CTYRAG 2 cut(s) 932, 1594
SphI GCATGC 1 cut(s) 787
SspMI CTAG 6 cut(s) 311, 1499, 1715, 2121, 2172, 2993
SstI GAGCTC 1 cut(s) 1221
StyD4I CCNGG 3 cut(s) 1624, 1839, 2439
StyI CCWWGG 2 cut(s) 2333, 2560
TaiI ACGT 1 cut(s) 119
TaqII GACCGA 1 cut(s) 2874
TatI WGTACW 3 cut(s) 439, 2640, 2970
TfiI GAWTC 7 cut(s) 829, 1464, 1675, 1693, 2306, 2510, 2534
Tru1I TTAA 7 cut(s) 512, 1364, 1437, 1595, 1725, 1754, 2205
Tru9I TTAA 7 cut(s) 512, 1364, 1437, 1595, 1725, 1754, 2205
TscAI CASTG 7 cut(s) 1141, 1330, 1474, 1834, 2050, 3334, 3417
TspGWI ACGGA 1 cut(s) 2124
TspRI CASTG 7 cut(s) 1141, 1330, 1474, 1834, 2050, 3334, 3417
Van91I CCANNNNNTGG 1 cut(s) 3052
Vha464I CTTAAG 1 cut(s) 1594
VpaK11BI GGWCC 5 cut(s) 249, 502, 1400, 2816, 2886
XapI RAATTY 4 cut(s) 205, 274, 331, 1360
XbaI TCTAGA 2 cut(s) 2120, 2171
XceI RCATGY 3 cut(s) 676, 787, 2647
XhoI CTCGAG 1 cut(s) 932
XmiI GTMKAC 2 cut(s) 864, 2668
XmnI GAANNNNTTC 2 cut(s) 495, 648
XspI CTAG 6 cut(s) 311, 1499, 1715, 2121, 2172, 2993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.