Rroxscaffold_7G00183330
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013816
Physical Location & Seq
Reverse (-)
22414651 .. 22420869
6219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183330.1

Sequence Viewer

Length: 3579 bp
ATGGCAATGATATATAATTGTCGAAAATGGTATCATGTCATGGTTGGGCAAATGAATGCATGGATTCCAATCACACTCAACAACACCGGACCATTGATACCACCAGCAGTACCGATAAGGTTACCCAGCAAAAGTTTTAGGGACATACCAGATAATTGGAGATTATCCGTGACAAATGTTGCATCATGTTATATATCTCAACTCAATCTCAAACCTTCTGAACAAGCTAGTCAAAGTTATAGACTAGATAATATTGCAATTGCAGTAGCACAAACTAGTAGGGTTGAAGAAAAGAATCAAGAAAAAGTGAGAGAGACCCAATGCAAAACCAAGAGGAGGGTTAAGGTTTTGAGGCAAAATTTGGCAATGCCAATGCTGTTGAGGCAGCAGCCCTCTAACACCTACAATCACCACCAAATGTCGAGGCCACTATCGTTCACCACCATCGATTTCTTCTTCCTTTTCATCTTCACACTCGCAACGTCTGCTGCTTCTTTGGCTGCTGCAAATGTTGACGTTCTGACATTACATACATGGCTTCAAGGCTCTACTACCTCACCCTCTGCTTCTGATTTCTCCAACTGGAACCCTTCAGACCCCAACCCTTGTAATTGGTCCTACATTACTTGTTCTTCTCAAAACTTTGTCACTGAAATTAATATCCAGTTTATTGAGCTAGCCCTTCCCTTTCCTTCAAATTTTTCCTCCCTTGCATTTCTTCAAAAGCTCATCATTTCTGGTGCTAATCTTACTGGAACAATCTCACCTGATATTGGATATTGCAAATCGCTTCTGGTGCTTGACGTAAGTTCAAACAGTCTTGTGGGTAGTATTCCTACAAGTATTGGCAGGCTTGAAAATCTTCAGGACCTGATCTTAAACTCTAACCAACTTACTGGTCAAGTACCAATGGAGCTTGGTGATTGCGCCAGCCTGAAGAATCTTCTCATATTTGATAACTACCTAAGTGGTTATCTTCCTGCTACACTCGGAAAGTTACTAAACTTGGAAGTCATTCGAGCTGGAGGGAACAAAGACATGTCAGGAAATATCCCGGATGAGCTTGGAAACTGCAAGAACTTGCAGGTGTTGGGGCTTGCTGATACTAAAGTTTCTGGTTTGATACCTTCATCATTAGGTAAGCTCAGCATGCTGCAGACGCTGTCGATTTATACTACTATGCTATCGGGTGAGATTCCACCGGATATTGGCAACTGTTCAGAACTCGTCAACTTGTTCTTGTATGAGAATGACCTTTCTGGTTCACTACCACCAGAGTTGGGCAAGCTTCATAAGCTGGAGAAGATGTTATTGTGGCAAAACAACCTTGATGGGACTATTCCTGAGGAAATTGGGAACTGCAGAAGCTTGAAGACCATAGATGTCTCTCTAAATTCTGTTTCTGGAAGCATACCTCAGTCTTTCGGAAACCTCTCAAATCTTGAGGAGCTTATGCTTAGTAACAACAACATCTCTGGTTCAATTCCCCCAGTTCTTTCCAATGCTACAAAACTTGTGCAGCTGCAGCTTGATACTAATCAGATTTCAGGCTTGATCCCAGCTGAGCTGGGAATGTTGAAGGAGCTCACAGTCTTTTTTGCTTGGCAGAACAAACTCGAAGGTAGTATTCCATCAGAATTGGCCGGTTGTGAGAGTCTTCAAGCTCTAGATCTCTCACACAATGCTCTCACTGGTAACATACCTTCTGGCCTATTTCAGCTGAAAAATTTAACCAAGCTTCTCTTGATTTCTAATGACATTTCGGGTCCAATACCTTCGGTGATTGGAAATTGTACTTCTCTTATCCGGCTAAGGCTTGTGCATAACAGAATCAGTGGGGAGATCCCAAAAGAAATTGGGTTCCTTGATAACCTCAGTTTCCTTGATCTGTCTGAAAACCGTCTTGTTGGTATCATACCAGAAGAAATTGGAAAATGCAGTTCATTACAGCTACTCAACTTAAGTAACAACACCCTTGGAGGTAATCTGCCTAGCTCCTTTTCTTCTCTCACAAGGCTTCAAGTACTGGATGTCTCTGTGAATCAATTTGTAGGTCAGATTCCTGAGAGTTACGGTGGACTTGCTTCACTTAATAGGCTCATCCTCCGTAGCAACTCCTTCACTGAGCCAATCCCCTCTTCACTTGGTAAATGTTCAAACCTTCAACTGCTTGATCTTAGTAGCAACAAGCTCACTGGGATGATCCCAGAGCAACTGTTTGAAATTGAAGCTCTTGACATTGCTTTGAACCTAAGTTTCAATTCTTTATATGGGATAATCCCACCACAGATTTCTGCTCTCAATAAGCTATCCATACTGGACCTTTCACACAATAAGCTTGAAGGTGATCTGTTGGCACTTTCCGGGCTTATAAATCTTGTTTCTTTGAACATCTCATACAACAACTTCACTGGCTATCTTCCTGACAGCAAGGTGTTTCGACAGTTGTCAGCAACAGACTTGGTGGGAAACAAAGGATTGTGCTCTAGAGGTCATGATCTATGTTTCCTCAGCAATGGTACAACAATGGCCATGTCTAGAAGTGGCAGTTTCAGGAGGTCATGGCTACTTAAATTGGCCATTGCATTGCTAATAGCCTTGACCATTGCTTTGTCCATTTTTGGGGTAGTTGCAGTATTTCGAGCACGAAAGATGAATGGAGAGGACAATGATTCTGAGATTGGAGTGGACTCATGGCCTTGGCAATTCACTCCATTTCAGAAGGTGAGTTTCTCAGTTGAGCAAGTTCTAAAATGTCTAGTAGAAACCAATGTAATAGGAAAGGGATGCTCAGGAGTTGTTTACCGGGCAGAAATCGAAAACGAAGACATTGCAGTAAAGAAGCTCTGGCCAACAACAACTGCTTACAGATATGATTGTCAAAGTAGAGGAGTTCGTGATTCCTTTTCGGCTGAGGTGAAGACCCTTGGCTCCATTCGCCACAAGAACATTGTTAGGTTCTTGGGTTGCTGTTGGAACCGAAACACAAGATTGCTTATGTATGAGTACATGCCAAATGGGAGCTTGGGTGGTTTACTTCATGAAAGAAGCGAGTATGGATACTCAATGAAGATAACAGAGAAAAGCGATGTGTACAGCTACGGCGTAGTTGTGCTAGAAGTGCTAACAGGGAAGCAACCAATTGATCCAACCATACCAGATGGACTCCACATTGTGGATTGGGTAAGGCAGAAAAGAGGGGGAGTTGAGGTACTTGATCAAAGCTTACGAGCACGACCAGAACCCGAGATTGAGGAAATGCTGCAGACATTAGGTGTGGCATTGCTGTGCATTAACCCTACCCCGGATGACAGACCAACCATGAAAGATGTAGCAGCAATGCTCAAAGAGATTAGGCAGGAGAGAGAGGAGTGCATGAAAGCTAACATGAGGCTTCTCAATGGATCCAATTCTGCAAATGATCATGGACAAGAAACTATGATCAGTTGCAGTGGAGGAACATCATCAGCTATGGTGAAGCAACAACACCCATACCCTCCAACAAGTACCAGCACAAGTTTCTCTGCTTCCTCATTGCTATACACTTCATCTCCCAATGTCAAAACAACTCTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1192

Amino Acids

130.85

Weight (kDa)

6.07

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 170 - 211 8.6e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 348 - 440 1.2e-08 Leucine-rich repeat region
LRR_14 PF23598 427 - 534 7.1e-09 Leucine-rich repeat region
LRR_8 PF13855 455 - 515 1e-07 Leucine rich repeat
LRR_4 PF12799 480 - 516 1e-05 Leucine Rich repeats (2 copies)
LRR_14 PF23598 542 - 630 5.4e-07 Leucine-rich repeat region
LRR_8 PF13855 600 - 659 8.4e-06 Leucine rich repeat
LRR_14 PF23598 604 - 821 7.5e-14 Leucine-rich repeat region
LRR_8 PF13855 624 - 683 5.2e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 923 - 1017 1e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 923 - 1016 1.2e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1022 - 1114 1.5e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48940
fragaria_vesca FvH4_2g19620
malus_domestica MD10G1169800.v1.1
prunus_persica Prupe.8G207600_v2.0.a1
pyrus_communis pycom10g14630
rosa_chinensis RchiOBHm_Chr6g0285351
rosa_laevigata RLG00000012651
rosa_multiflora Rmu_co8152086.1_g000001 Rmu_co8221716.1_g000001 Rmu_sc0012287.1_g000004
rosa_roxburghii Rroxscaffold_7G00183330
rosa_rugosa Rorug06G0173900
rosa_samantha Rh6AG284500 Rh6BG285700 Rh6CG286800 Rh6DG279800
rosa_wichuraiana Rw6G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2372
AarI CACCTGC 1 cut(s) 1075
Acc36I ACCTGC 1 cut(s) 1075
AccB7I CCANNNNNTGG 1 cut(s) 3175
AclWI GGATC 6 cut(s) 1549, 1837, 2197, 3140, 3399, 3412
AcoI YGGCCR 4 cut(s) 1641, 2529, 2577, 2849
AcsI RAATTY 4 cut(s) 358, 697, 1393, 1726
AcuI CTGAAG 3 cut(s) 576, 848, 956
AdeI CACNNNGTG 1 cut(s) 3175
AfaI GTAC 9 cut(s) 111, 906, 1795, 2025, 2521, 3008, 3095, 3213, 3508
AfiI CCNNNNNNNGG 7 cut(s) 336, 773, 1208, 1280, 2622, 3175, 3304
AflII CTTAAG 1 cut(s) 1960
AflIII ACRYGT 1 cut(s) 1038
AhlI ACTAGT 1 cut(s) 275
AjuI GAANNNNNNNTTGG 2 cut(s) 2122, 2154
Alw21I GWGCWC 4 cut(s) 1587, 2486, 2647, 3235
Alw26I GTCTC 3 cut(s) 308, 1390, 2038
AlwI GGATC 6 cut(s) 1549, 1837, 2197, 3140, 3399, 3412
AlwNI CAGNNNCTG 2 cut(s) 871, 1162
Ama87I CYCGRG 1 cut(s) 3245
AoxI GGCC 7 cut(s) 425, 1641, 1708, 2529, 2577, 2696, 2849
ApoI RAATTY 4 cut(s) 358, 697, 1393, 1726
ArsI GACNNNNNNTTYG 2 cut(s) 2593, 2625
AseI ATTAAT 1 cut(s) 657
Asp700I GAANNNNTTC 2 cut(s) 861, 1014
AspLEI GCGC 1 cut(s) 929
AspS9I GGNCC 5 cut(s) 89, 615, 868, 1766, 2320
AsuC2I CCSGG 4 cut(s) 1055, 2365, 2807, 3305
AsuNHI GCTAGC 1 cut(s) 676
AvaI CYCGRG 1 cut(s) 3245
AvaII GGWCC 5 cut(s) 89, 615, 868, 1766, 2320
AxyI CCTNAGG 1 cut(s) 1344
BaeI ACNNNNGTAYC 2 cut(s) 1095, 1128
BalI TGGCCA 3 cut(s) 2531, 2579, 2851
BamHI GGATCC 1 cut(s) 3404
BanII GRGCYC 1 cut(s) 1587
BarI GAAGNNNNNNTAC 2 cut(s) 1687, 1719
BbsI GAAGAC 4 cut(s) 1379, 1649, 2832, 2927
Bbv12I GWGCWC 4 cut(s) 1587, 2486, 2647, 3235
BbvCI CCTCAGC 2 cut(s) 2510, 2913
BccI CCATC 4 cut(s) 452, 1325, 1639, 3155
BceAI ACGGC 1 cut(s) 3118
BcgI CGANNNNNNTGC 2 cut(s) 2813, 2847
BciVI GTATCC 1 cut(s) 3053
BclI TGATCA 3 cut(s) 3217, 3421, 3441
BcnI CCSGG 4 cut(s) 1055, 2365, 2807, 3305
BcoDI GTCTC 3 cut(s) 308, 1390, 2038
BcuI ACTAGT 1 cut(s) 275
BfmI CTRYAG 4 cut(s) 1154, 1360, 1523, 3263
BfrI CTTAAG 1 cut(s) 1960
BfuAI ACCTGC 1 cut(s) 1075
BfuI GTATCC 1 cut(s) 3053
BglII AGATCT 1 cut(s) 1669
BlpI GCTNAGC 2 cut(s) 1145, 1563
BmcAI AGTACT 1 cut(s) 2025
Bme1390I CCNGG 4 cut(s) 1055, 2365, 2807, 3305
Bme18I GGWCC 5 cut(s) 89, 615, 868, 1766, 2320
BmeT110I CYCGRG 1 cut(s) 3245
BmgT120I GGNCC 5 cut(s) 89, 615, 868, 1766, 2320
BmiI GGNNCC 6 cut(s) 587, 1767, 1862, 2932, 2978, 3406
BmrFI CCNGG 4 cut(s) 1055, 2365, 2807, 3305
BmrI ACTGGG 2 cut(s) 1484, 2205
BmsI GCATC 2 cut(s) 191, 2777
BmtI GCTAGC 1 cut(s) 680
BmuI ACTGGG 2 cut(s) 1484, 2205
BoxI GACNNNNGTC 1 cut(s) 2446
BpiI GAAGAC 4 cut(s) 1379, 1649, 2832, 2927
BpmI CTGGAG 2 cut(s) 1044, 1319
Bpu10I CCTNAGC 4 cut(s) 1811, 2510, 2791, 2913
Bpu1102I GCTNAGC 2 cut(s) 1145, 1563
BpuEI CTTGAG 1 cut(s) 1463
BpuMI CCSGG 4 cut(s) 1055, 2365, 2807, 3305
Bsa29I ATCGAT 1 cut(s) 447
BsaBI GATNNNNATC 2 cut(s) 68, 1536
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 4 cut(s) 1975, 2699, 2926, 3303
BsaWI WCCGGW 2 cut(s) 86, 1201
BsaXI ACNNNNNCTCC 8 cut(s) 1292, 1322, 3013, 3043, 3195, 3225, 3535, 3565
Bsc4I CCNNNNNNNGG 7 cut(s) 336, 773, 1208, 1280, 2622, 3175, 3304
Bse118I RCCGGY 1 cut(s) 1643
Bse21I CCTNAGG 1 cut(s) 1344
Bse8I GATNNNNATC 2 cut(s) 68, 1536
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 4 cut(s) 1975, 2699, 2926, 3303
BseGI GGATG 6 cut(s) 1063, 2035, 2100, 2205, 2792, 3313
BseJI GATNNNNATC 2 cut(s) 68, 1536
BseLI CCNNNNNNNGG 7 cut(s) 336, 773, 1208, 1280, 2622, 3175, 3304
BseRI GAGGAG 4 cut(s) 349, 1460, 2904, 3383
BseYI CCCAGC 3 cut(s) 125, 1558, 1567
BsgI GTGCAG 1 cut(s) 1538
BshFI GGCC 7 cut(s) 427, 1643, 1710, 2531, 2579, 2698, 2851
BshVI ATCGAT 1 cut(s) 447
BsiHKAI GWGCWC 4 cut(s) 1587, 2486, 2647, 3235
BsiHKCI CYCGRG 1 cut(s) 3245
BsiSI CCGG 8 cut(s) 87, 1055, 1202, 1644, 1807, 2364, 2806, 3305
BslFI GGGAC 2 cut(s) 155, 1348
BslI CCNNNNNNNGG 7 cut(s) 336, 773, 1208, 1280, 2622, 3175, 3304
BsmAI GTCTC 3 cut(s) 308, 1390, 2038
BsmFI GGGAC 2 cut(s) 155, 1348
BsmI GAATGC 1 cut(s) 61
BsnI GGCC 7 cut(s) 427, 1643, 1710, 2531, 2579, 2698, 2851
Bso31I GGTCTC 1 cut(s) 308
BsoBI CYCGRG 1 cut(s) 3245
Bsp1286I GDGCHC 4 cut(s) 1587, 2486, 2647, 3235
Bsp1407I TGTACA 1 cut(s) 3093
Bsp1720I GCTNAGC 2 cut(s) 1145, 1563
BspANI GGCC 7 cut(s) 427, 1643, 1710, 2531, 2579, 2698, 2851
BspDI ATCGAT 1 cut(s) 447
BspHI TCATGA 2 cut(s) 2494, 3040
BspLI GGNNCC 6 cut(s) 587, 1767, 1862, 2932, 2978, 3406
BspMAI CTGCAG 4 cut(s) 1158, 1364, 1527, 3267
BspMI ACCTGC 1 cut(s) 1075
BspOI GCTAGC 1 cut(s) 680
BspPI GGATC 6 cut(s) 1549, 1837, 2197, 3140, 3399, 3412
BspTI CTTAAG 1 cut(s) 1960
BspTNI GGTCTC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 1643
BsrGI TGTACA 1 cut(s) 3093
BssAI RCCGGY 1 cut(s) 1643
BssECI CCNNGG 4 cut(s) 1975, 2699, 2926, 3303
BssT1I CCWWGG 3 cut(s) 1975, 2699, 2926
Bst4CI ACNGT 7 cut(s) 818, 1217, 1591, 1901, 2075, 2217, 2445
Bst6I CTCTTC 1 cut(s) 2143
BstAFI CTTAAG 1 cut(s) 1960
BstAPI GCANNNNNTGC 1 cut(s) 485
BstAUI TGTACA 1 cut(s) 3093
BstC8I GCNNGC 6 cut(s) 678, 851, 931, 1098, 1151, 1286
BstEII GGTNACC 1 cut(s) 120
BstF5I GGATG 6 cut(s) 1063, 2035, 2100, 2205, 2792, 3313
BstHHI GCGC 1 cut(s) 929
BstMAI GTCTC 3 cut(s) 308, 1390, 2038
BstNSI RCATGY 3 cut(s) 1042, 1153, 3013
BstPAI GACNNNNGTC 1 cut(s) 2446
BstPI GGTNACC 1 cut(s) 120
BstSCI CCNGG 4 cut(s) 1053, 2363, 2805, 3303
BstSFI CTRYAG 4 cut(s) 1154, 1360, 1523, 3263
BstV2I GAAGAC 4 cut(s) 1379, 1649, 2832, 2927
BstX2I RGATCY 3 cut(s) 1669, 1842, 3404
BstXI CCANNNNNNTGG 2 cut(s) 156, 896
BstYI RGATCY 3 cut(s) 1669, 1842, 3404
Bsu15I ATCGAT 1 cut(s) 447
Bsu36I CCTNAGG 1 cut(s) 1344
BsuI GTATCC 1 cut(s) 3053
BsuRI GGCC 7 cut(s) 427, 1643, 1710, 2531, 2579, 2698, 2851
BsuTUI ATCGAT 1 cut(s) 447
BtgZI GCGATG 1 cut(s) 3102
BtsCI GGATG 6 cut(s) 1063, 2035, 2100, 2205, 2792, 3313
BtsI GCAGTG 1 cut(s) 3457
BtsIMutI CAGTG 7 cut(s) 648, 1689, 1840, 2121, 2193, 2409, 3457
BveI ACCTGC 1 cut(s) 1075
Cac8I GCNNGC 6 cut(s) 678, 851, 931, 1098, 1151, 1286
CaiI CAGNNNCTG 2 cut(s) 871, 1162
CciI TCATGA 2 cut(s) 2494, 3040
CfoI GCGC 1 cut(s) 929
Cfr10I RCCGGY 1 cut(s) 1643
Cfr13I GGNCC 5 cut(s) 89, 615, 868, 1766, 2320
ClaI ATCGAT 1 cut(s) 447
CseI GACGC 1 cut(s) 1168
Csp6I GTAC 9 cut(s) 110, 905, 1794, 2024, 2520, 3007, 3094, 3212, 3507
CspCI CAANNNNNGTGG 2 cut(s) 1260, 1295
CviQI GTAC 9 cut(s) 110, 905, 1794, 2024, 2520, 3007, 3094, 3212, 3507
DraIII CACNNNGTG 1 cut(s) 3175
EaeI YGGCCR 4 cut(s) 1641, 2529, 2577, 2849
Eam1104I CTCTTC 1 cut(s) 2143
EarI CTCTTC 1 cut(s) 2143
Ecl136II GAGCTC 1 cut(s) 1585
Eco130I CCWWGG 3 cut(s) 1975, 2699, 2926
Eco24I GRGCYC 1 cut(s) 1587
Eco31I GGTCTC 1 cut(s) 308
Eco47I GGWCC 5 cut(s) 89, 615, 868, 1766, 2320
Eco53kI GAGCTC 1 cut(s) 1585
Eco57I CTGAAG 3 cut(s) 576, 848, 956
Eco81I CCTNAGG 1 cut(s) 1344
Eco88I CYCGRG 1 cut(s) 3245
Eco91I GGTNACC 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 1585
EcoO109I RGGNCCY 1 cut(s) 868
EcoO65I GGTNACC 1 cut(s) 120
EcoT14I CCWWGG 3 cut(s) 1975, 2699, 2926
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 1587
ErhI CCWWGG 3 cut(s) 1975, 2699, 2926
FalI AAGNNNNNCTT 2 cut(s) 1727, 1759
FaqI GGGAC 2 cut(s) 155, 1348
FbaI TGATCA 3 cut(s) 3217, 3421, 3441
FokI GGATG 6 cut(s) 1070, 2042, 2087, 2212, 2799, 3320
FriOI GRGCYC 1 cut(s) 1587
GlaI GCGC 1 cut(s) 928
GsaI CCCAGC 3 cut(s) 129, 1562, 1571
GsuI CTGGAG 2 cut(s) 1044, 1319
HaeIII GGCC 7 cut(s) 427, 1643, 1710, 2531, 2579, 2698, 2851
HapII CCGG 8 cut(s) 87, 1055, 1202, 1644, 1807, 2364, 2806, 3305
HgaI GACGC 1 cut(s) 1168
HhaI GCGC 1 cut(s) 929
Hin6I GCGC 1 cut(s) 927
HinP1I GCGC 1 cut(s) 927
HincII GTYRAC 2 cut(s) 514, 1231
HindII GTYRAC 2 cut(s) 514, 1231
HindIII AAGCTT 5 cut(s) 1286, 1366, 1736, 2336, 3223
HpaII CCGG 8 cut(s) 87, 1055, 1202, 1644, 1807, 2364, 2806, 3305
Hpy166II GTNNAC 9 cut(s) 438, 514, 1231, 1265, 2078, 2689, 2803, 3035, 3094
Hpy8I GTNNAC 9 cut(s) 438, 514, 1231, 1265, 2078, 2689, 2803, 3035, 3094
HpyCH4III ACNGT 7 cut(s) 818, 1217, 1591, 1901, 2075, 2217, 2445
HpyCH4IV ACGT 3 cut(s) 482, 516, 804
HpySE526I ACGT 3 cut(s) 482, 516, 804
HspAI GCGC 1 cut(s) 927
Ksp22I TGATCA 3 cut(s) 3217, 3421, 3441
LmnI GCTCC 6 cut(s) 913, 1447, 1582, 2000, 2936, 3021
LweI GCATC 2 cut(s) 191, 2777
MaeII ACGT 3 cut(s) 482, 516, 804
MaeIII GTNAC 8 cut(s) 120, 169, 646, 996, 1460, 1694, 1964, 2069
MfeI CAATTG 2 cut(s) 258, 3141
MflI RGATCY 3 cut(s) 1669, 1842, 3404
MhlI GDGCHC 4 cut(s) 1587, 2486, 2647, 3235
MlsI TGGCCA 3 cut(s) 2531, 2579, 2851
MluNI TGGCCA 3 cut(s) 2531, 2579, 2851
MlyI GAGTC 3 cut(s) 1663, 2684, 3159
MmeI TCCRAC 4 cut(s) 603, 2954, 3173, 3524
Mox20I TGGCCA 3 cut(s) 2531, 2579, 2851
Mph1103I ATGCAT 1 cut(s) 61
MroXI GAANNNNTTC 2 cut(s) 861, 1014
MscI TGGCCA 3 cut(s) 2531, 2579, 2851
MseI TTAA 8 cut(s) 342, 657, 878, 1730, 1961, 2091, 2571, 3294
MslI CAYNNNNRTG 2 cut(s) 2198, 3286
Msp20I TGGCCA 3 cut(s) 2531, 2579, 2851
MspA1I CMGCKG 3 cut(s) 1522, 1562, 1720
MspCI CTTAAG 1 cut(s) 1960
MspI CCGG 8 cut(s) 87, 1055, 1202, 1644, 1807, 2364, 2806, 3305
MspR9I CCNGG 4 cut(s) 1055, 2365, 2807, 3305
MunI CAATTG 2 cut(s) 258, 3141
Mva1269I GAATGC 1 cut(s) 61
NciI CCSGG 4 cut(s) 1055, 2365, 2807, 3305
NheI GCTAGC 1 cut(s) 676
NlaIV GGNNCC 6 cut(s) 587, 1767, 1862, 2932, 2978, 3406
NmuCI GTSAC 2 cut(s) 169, 646
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 3 cut(s) 1042, 1153, 3013
PaeI GCATGC 1 cut(s) 1153
PagI TCATGA 2 cut(s) 2494, 3040
PaqCI CACCTGC 1 cut(s) 1075
PciI ACATGT 1 cut(s) 1038
PctI GAATGC 1 cut(s) 61
PdmI GAANNNNTTC 2 cut(s) 861, 1014
PfeI GAWTC 9 cut(s) 64, 295, 940, 1195, 1830, 2041, 2059, 2672, 2900
PflFI GACNNNGTC 1 cut(s) 1162
PflMI CCANNNNNTGG 1 cut(s) 3175
PfoI TCCNGGA 1 cut(s) 1053
PleI GAGTC 3 cut(s) 1662, 2684, 3159
PpsI GAGTC 3 cut(s) 1662, 2684, 3159
PpuMI RGGWCCY 1 cut(s) 868
PscI ACATGT 1 cut(s) 1038
PshAI GACNNNNGTC 1 cut(s) 2446
PshBI ATTAAT 1 cut(s) 657
PsiI TTATAA 1 cut(s) 2372
Psp124BI GAGCTC 1 cut(s) 1587
Psp5II RGGWCCY 1 cut(s) 868
PspEI GGTNACC 1 cut(s) 120
PspFI CCCAGC 3 cut(s) 125, 1558, 1567
PspN4I GGNNCC 6 cut(s) 587, 1767, 1862, 2932, 2978, 3406
PspPI GGNCC 5 cut(s) 89, 615, 868, 1766, 2320
PspPPI RGGWCCY 1 cut(s) 868
PsrI GAACNNNNNNTAC 2 cut(s) 2381, 2413
PstI CTGCAG 4 cut(s) 1158, 1364, 1527, 3267
PstNI CAGNNNCTG 2 cut(s) 871, 1162
PsuI RGATCY 3 cut(s) 1669, 1842, 3404
PsyI GACNNNGTC 1 cut(s) 1162
PvuII CAGCTG 3 cut(s) 1522, 1562, 1720
RsaI GTAC 9 cut(s) 111, 906, 1795, 2025, 2521, 3008, 3095, 3213, 3508
RsaNI GTAC 9 cut(s) 110, 905, 1794, 2024, 2520, 3007, 3094, 3212, 3507
RseI CAYNNNNRTG 2 cut(s) 2198, 3286
SacI GAGCTC 1 cut(s) 1587
SaqAI TTAA 8 cut(s) 342, 657, 878, 1730, 1961, 2091, 2571, 3294
Sau96I GGNCC 5 cut(s) 89, 615, 868, 1766, 2320
ScaI AGTACT 1 cut(s) 2025
SchI GAGTC 3 cut(s) 1663, 2684, 3159
ScrFI CCNGG 4 cut(s) 1055, 2365, 2807, 3305
SduI GDGCHC 4 cut(s) 1587, 2486, 2647, 3235
SfaNI GCATC 2 cut(s) 191, 2777
SfcI CTRYAG 4 cut(s) 1154, 1360, 1523, 3263
SinI GGWCC 5 cut(s) 89, 615, 868, 1766, 2320
SmiMI CAYNNNNRTG 2 cut(s) 2198, 3286
SmlI CTYRAG 2 cut(s) 1442, 1960
SmoI CTYRAG 2 cut(s) 1442, 1960
SpeI ACTAGT 1 cut(s) 275
SphI GCATGC 1 cut(s) 1153
SspI AATATT 1 cut(s) 253
SstI GAGCTC 1 cut(s) 1587
StyD4I CCNGG 4 cut(s) 1053, 2363, 2805, 3303
StyI CCWWGG 3 cut(s) 1975, 2699, 2926
TaaI ACNGT 7 cut(s) 818, 1217, 1591, 1901, 2075, 2217, 2445
TaiI ACGT 3 cut(s) 485, 519, 807
TaqI TCGA 9 cut(s) 22, 422, 447, 1018, 1166, 1617, 2440, 2641, 2817
TatI WGTACW 4 cut(s) 1793, 2023, 3006, 3093
TfiI GAWTC 9 cut(s) 64, 295, 940, 1195, 1830, 2041, 2059, 2672, 2900
Tru1I TTAA 8 cut(s) 342, 657, 878, 1730, 1961, 2091, 2571, 3294
Tru9I TTAA 8 cut(s) 342, 657, 878, 1730, 1961, 2091, 2571, 3294
TscAI CASTG 7 cut(s) 655, 1696, 1840, 2128, 2200, 2416, 3457
TseFI GTSAC 2 cut(s) 169, 646
Tsp45I GTSAC 2 cut(s) 169, 646
TspGWI ACGGA 2 cut(s) 157, 2096
TspRI CASTG 7 cut(s) 655, 1696, 1840, 2128, 2200, 2416, 3457
Tth111I GACNNNGTC 1 cut(s) 1162
Van91I CCANNNNNTGG 1 cut(s) 3175
Vha464I CTTAAG 1 cut(s) 1960
VpaK11BI GGWCC 5 cut(s) 89, 615, 868, 1766, 2320
VspI ATTAAT 1 cut(s) 657
XapI RAATTY 4 cut(s) 358, 697, 1393, 1726
XbaI TCTAGA 3 cut(s) 1666, 2486, 2537
XceI RCATGY 3 cut(s) 1042, 1153, 3013
XmnI GAANNNNTTC 2 cut(s) 861, 1014
ZrmI AGTACT 1 cut(s) 2025
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.