RLG00000012651
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013816
Physical Location & Seq
Reverse (-)
20926031 .. 20929612
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012651

Sequence Viewer

Length: 3453 bp
ATGCCAATGCTGTTGAGGCAGCAGCCCTCTAACACCTACAATCACCACCAAATGTCGAGGCCATTATCGTTCACCACCATCGATTTCTTCTTCCTCTTCGTCTTCACACTAGCAACGTCTGCTGCTTTGGCTGCTGCAAATGTTGAGGTTCTGACATTACATACATGGCTTCAGGGCTCTACTACCTCGCCCTATTCTTCCGATTTCTCCAACTGGAACCCTTCAGACCCCAACCCTTGTAATTGGTCCTACATTACTTGTTCACCCCAAAACTTTGTCACTGAGATCAATATCCAGTTTATTGAGCTAGCCCTTCTCTTTCCTTCAAATTTTTCCTCCCTTGCATTTCTTCAAAAGCTCATCATTTCTGGTGCTAATCTTACTGGAACAATCTCACCTGATATTGGATATTGCAAATCACTTATGGTGCTTGATGTAAGTTCCAACAGTCTTGTGGGTAGTATTCCTACAAGTATTGGCAGGCTTGAAAATCTTCAGGACCTGATCTTAAACTCTAACCAGCTTACTGGTCAAGTACCAATGGAGCTTGGTGATTGCGCCAGCCTGAAGAATCTTCTCATATTTGATAACTATCTAAGTGGTTACCTTCCTGCTACACTCGGAAAGTTACTAAACTTGGAAGTCATTCGAGCTGGAGGGAACAAAGACATGTCAGGAAATATCCCGGATGAGCTTGGAAACTGCAAGAACTTGCAGGTGTTGGGGCTTGCTGATACTAAAGTTTCTGGTTTGATACCTTCATCATTGGGTAAGCTCAGCATGCTGCAGACGCTGTCGATTTATACTACTATGCTATCGGGTGAGATTCCACCGGATATTGGCAACTGTTCAGAACTTGTCAACTTGTTCTTGTATGAGAATGACCTTTCTGGTTCACTACCACCAGAGTTGGGAAAGCTTCATAAGCTGGAGAAGATGTTATTGTGGCAAAACAACCTTGACGGCACTATTCCTGAGGAAATTGGGAACTGCAGAAGCTTGAAGACCATAGATGTCTCTCTAAATTCTGTTTCTGGAAGCATACCTCAGTCTTTCGGAAACCTCTCAAATCTTGAGGAGCTTATGCTTAGTAACAACAACATCTCTGGTTCAATCCCCTCAGTTCTTTCCAATGCTACAAAACTTGTGCAGCTGCAGCTTGATACTAATCAGATTTCAGGTTTGATCCCAGCTGAGCTGGGAATGTTGAAGGAGCTCACAGTCTTTTTTGCTTGGCAGAACAAACTCGAAGGAAGCATTCCATCAGAACTGGCCGGTTGTGAGAGTCTTCAAGCTCTAGATCTCTCACACAATGCTCTCACTGGTAACATACCTTCTGGCCTATTTCAGCTGCAAAATTTAACCAAGCTTCTCTTGATTTCTAATGACATTTCGGGTCCAATACCTTCGATGATTGGAAATTGTACTTCTCTTATCCGGCTAAGGCTTGTGCATAACAGAATCAGTGGAGAGATCCCAAAAGAAATTGGGTTCCTTGATAACCTCAGTTTCCTTGATCTGTCTGAAAACCGTCTTGTTGGTATCGTACCAGAAGAAATTGGAAAATGCAGTTCATTACAGCTACTCAACTTAAGTAACAACACCCTTGGAGGTAATCTGCCTAGCTCCTTTTCTTCTCTCACAAGGCTTCAAGTATTGGATGTCTCTGTGAATCAATTTGTAGGTCAGATTCCTGAGAGTTACGGTGGACTTGCTTCACTTAATAGGCTCATCCTCCGTAGCAACTCCTTAACTGAGCCAATCCCCTCTTCACTTGGTAAATGTTCAAACCTTCAACTGCTTGACCTTAGTAGCAACAAGCTTACTGGGATGATCCCAGAGCAACTGTTTGAAATTGAAGCTCTTGACATTGCTTTGAACCTGAGTTTCAATGCTTTATATGGGATAATCCCACCACAGATTTCTGCTCTCAATAAGCTATCCATACTAGACCTTTCACACAATAAGCTTGAAGGTGATCTATTGGCACTTTCCGGGCTTATAAATCTTGTTTCTTTGAACCTCTCATACAACAACTTCACTGGCTATCTTCCTGACAGCAAGGTGTTTCGACAGTTGTCAGCAACAGACTTGGTGGGAAACAAAGGATTGTGCTCTAGAGGTCATGATCTATGTTTCCTCAGCAATGGTACAACAATGGCCATGTCTAGAAGTGGCAGTTTCAGGAGGTCATGGCGACTTAAATTGGCCATTGCATCGCTAATAGCCTTGACCATTGCTTTGTCCATTTTTGGGGTAGTTGCAGTATTTCGAGCACGAAAGATGAATGGAGAGGACAATGATTCTGAGATTGGAGGGGACTCATGGCCTTGGCAATTCACTCCATTTCAAAAGGTAAGTTTCTCAGTTGAGCAAGTTCTAAAATGTCTAGTAGAAACCAATGTAATAGGAAAGGGATGCTCAGGAGTTGTTTACCGGGCAGAAATGGAAAACGAAGACATTGCAGTAAAGAAGCTCTGGCCAACAGCAACTGCTTCCAAATATGATTGTCAAAGTAGAGGAGTTCGTGATTCCTTTTCAGCTGAGGTGAAGACCCTTGGCTCCATTCGCCACAAGAACATTGTTAGGTTCTTGGGTTGCTGTTGGAACCGAAACACAAGATTGCTTATGTATGAGTACATGCCAAATGGGAGCTTGGGTGGTTTACTTCATGAAAGAAGTGGTAACTGCTTAGAATGGGACATCAGATATCGCATAGTACTAGGGGCCGCGCAAGGCTTGGCTTATTTGCACCATGACTCTGTTCCTCCTATAGTTCACAGAGACATTAAGGCCAACAACATTCTCATTGGTCCAGATTTTGAGCCCTGCATTGCTGATTTTGGGCTTGCCAAGCTCGTTGATGAAGGAGACTTTGCTCGGTCCTCCAACACGGTTGCAGGTTCTTATGGCTACATTGCTCCTGAGTATGGATACTCAATGAAGATAACAGAGAAAAGCGATGTATACAGCTACGGCGTAGTTGTGCTAGAAGTGCTAACAGGGAAGCAACCAATTGATCCAACCATACCAGATGGACTCCACATTGTGGATTGGGTAAGGCAGAAGAGAGGGGGAATTGAGGTACTTGATCAAAGCTTACGAGCACGACCAGAACCCGAGATTGAGGAAATGCTGCAGACATTAGGTGTGGCATTGCTGTGCATTAACCCTACCCCAGATGACAGACCAACCATGAAAGATGTAGCAGCAATGCTCAAAGAGATTAGGCAGGAGAGAGAGGAGTGCATGAAAGCTAACATGAGGCTTCTCAATGGGTCGAATTCTGGAAATGATCATGGACAAGAAACTATGATCAGTTGCAGTGGAGGAACATCATCAGCTATGGTGCAGCAACAACATCCATACCCTCCAACAAGTACCAGCACAAGTTTCTCTGCTTCCTCATTGCTATACACTTCATCTTCCAATGTCAAAACAACTCTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1151

Amino Acids

125.46

Weight (kDa)

5.3

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 49 - 88 9.2e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 215 - 316 8.6e-09 Leucine-rich repeat region
LRR_14 PF23598 323 - 409 3.3e-08 Leucine-rich repeat region
LRR_8 PF13855 332 - 392 5.8e-08 Leucine rich repeat
LRR_14 PF23598 419 - 507 4.3e-07 Leucine-rich repeat region
LRR_8 PF13855 477 - 536 5.1e-06 Leucine rich repeat
LRR_14 PF23598 481 - 698 4.7e-14 Leucine-rich repeat region
LRR_8 PF13855 501 - 560 2.3e-07 Leucine rich repeat
LRR_8 PF13855 573 - 633 5.5e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 800 - 1072 1.2e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 800 - 1070 2.1e-40 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48940
fragaria_vesca FvH4_2g19620
malus_domestica MD10G1169800.v1.1
prunus_persica Prupe.8G207600_v2.0.a1
pyrus_communis pycom10g14630
rosa_chinensis RchiOBHm_Chr6g0285351
rosa_laevigata RLG00000012651
rosa_multiflora Rmu_co8152086.1_g000001 Rmu_co8221716.1_g000001 Rmu_sc0012287.1_g000004
rosa_roxburghii Rroxscaffold_7G00183330
rosa_rugosa Rorug06G0173900
rosa_samantha Rh6AG284500 Rh6BG285700 Rh6CG286800 Rh6DG279800
rosa_wichuraiana Rw6G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2003
AarI CACCTGC 1 cut(s) 706
Acc36I ACCTGC 2 cut(s) 706, 2891
AccB7I CCANNNNNTGG 1 cut(s) 3049
AccI GTMKAC 1 cut(s) 2967
AccII CGCG 1 cut(s) 2732
AciI CCGC 1 cut(s) 2730
AclWI GGATC 4 cut(s) 1180, 1468, 1828, 3014
AcoI YGGCCR 4 cut(s) 1272, 2160, 2208, 2480
AcsI RAATTY 4 cut(s) 328, 1024, 1357, 3283
AcuI CTGAAG 4 cut(s) 155, 207, 479, 587
AdeI CACNNNGTG 1 cut(s) 3049
AfaI GTAC 8 cut(s) 537, 1426, 1548, 2152, 2639, 2721, 3087, 3382
AfiI CCNNNNNNNGG 6 cut(s) 404, 839, 911, 2253, 2930, 3049
AflII CTTAAG 1 cut(s) 1591
AflIII ACRYGT 1 cut(s) 669
AjuI GAANNNNNNNTTGG 2 cut(s) 1753, 1785
Alw21I GWGCWC 4 cut(s) 1218, 2117, 2278, 3109
Alw26I GTCTC 4 cut(s) 1021, 1669, 2778, 2865
AlwI GGATC 4 cut(s) 1180, 1468, 1828, 3014
AlwNI CAGNNNCTG 3 cut(s) 502, 793, 2492
Ama87I CYCGRG 1 cut(s) 3119
AoxI GGCC 9 cut(s) 59, 1272, 1339, 2160, 2208, 2327, 2480, 2727, 2793
ApoI RAATTY 4 cut(s) 328, 1024, 1357, 3283
ArsI GACNNNNNNTTYG 2 cut(s) 2224, 2256
Asp700I GAANNNNTTC 2 cut(s) 492, 645
AspLEI GCGC 2 cut(s) 560, 2734
AspS9I GGNCC 6 cut(s) 246, 499, 1397, 2727, 2813, 2883
AsuC2I CCSGG 3 cut(s) 686, 1996, 2438
AsuHPI GGTGA 8 cut(s) 35, 64, 255, 387, 563, 833, 1988, 2560
AsuNHI GCTAGC 1 cut(s) 307
AvaI CYCGRG 1 cut(s) 3119
AvaII GGWCC 5 cut(s) 246, 499, 1397, 2813, 2883
AxyI CCTNAGG 1 cut(s) 975
BaeI ACNNNNGTAYC 2 cut(s) 726, 759
BalI TGGCCA 3 cut(s) 2162, 2210, 2482
BanII GRGCYC 3 cut(s) 179, 1218, 2829
BarI GAAGNNNNNNTAC 2 cut(s) 1318, 1350
BbsI GAAGAC 5 cut(s) 94, 1010, 1280, 2463, 2558
Bbv12I GWGCWC 4 cut(s) 1218, 2117, 2278, 3109
BbvCI CCTCAGC 2 cut(s) 2141, 2544
BccI CCATC 3 cut(s) 86, 1270, 3029
BceAI ACGGC 2 cut(s) 979, 2992
BcgI CGANNNNNNTGC 2 cut(s) 2444, 2478
BciVI GTATCC 1 cut(s) 2927
BclI TGATCA 3 cut(s) 3091, 3295, 3315
BcnI CCSGG 3 cut(s) 686, 1996, 2438
BcoDI GTCTC 4 cut(s) 1021, 1669, 2778, 2865
BfmI CTRYAG 5 cut(s) 785, 991, 1154, 2772, 3137
BfrI CTTAAG 1 cut(s) 1591
BfuAI ACCTGC 2 cut(s) 706, 2891
BfuI GTATCC 1 cut(s) 2927
BglII AGATCT 1 cut(s) 1300
BlpI GCTNAGC 2 cut(s) 776, 1194
BmcAI AGTACT 1 cut(s) 2721
Bme1390I CCNGG 3 cut(s) 686, 1996, 2438
Bme18I GGWCC 5 cut(s) 246, 499, 1397, 2813, 2883
BmeT110I CYCGRG 1 cut(s) 3119
BmgT120I GGNCC 6 cut(s) 246, 499, 1397, 2727, 2813, 2883
BmiI GGNNCC 6 cut(s) 218, 1398, 1493, 2563, 2609, 2728
BmrFI CCNGG 3 cut(s) 686, 1996, 2438
BmrI ACTGGG 1 cut(s) 1836
BmsI GCATC 2 cut(s) 2225, 2408
BmtI GCTAGC 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 1836
BoxI GACNNNNGTC 1 cut(s) 2077
BpiI GAAGAC 5 cut(s) 94, 1010, 1280, 2463, 2558
BpmI CTGGAG 2 cut(s) 675, 950
Bpu10I CCTNAGC 4 cut(s) 1442, 2141, 2422, 2544
Bpu1102I GCTNAGC 2 cut(s) 776, 1194
BpuEI CTTGAG 1 cut(s) 1094
BpuMI CCSGG 3 cut(s) 686, 1996, 2438
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 3 cut(s) 290, 591, 1167
BsaJI CCNNGG 3 cut(s) 1606, 2330, 2557
BsaWI WCCGGW 1 cut(s) 832
BsaXI ACNNNNNCTCC 4 cut(s) 923, 953, 2644, 2674
Bsc4I CCNNNNNNNGG 6 cut(s) 404, 839, 911, 2253, 2930, 3049
Bse118I RCCGGY 1 cut(s) 1274
Bse1I ACTGG 8 cut(s) 218, 295, 388, 532, 1275, 1327, 1831, 2047
Bse21I CCTNAGG 1 cut(s) 975
Bse8I GATNNNNATC 3 cut(s) 290, 591, 1167
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 3 cut(s) 1606, 2330, 2557
BseGI GGATG 6 cut(s) 694, 1666, 1731, 1836, 2423, 3361
BseJI GATNNNNATC 3 cut(s) 290, 591, 1167
BseLI CCNNNNNNNGG 6 cut(s) 404, 839, 911, 2253, 2930, 3049
BseNI ACTGG 8 cut(s) 218, 295, 388, 532, 1275, 1327, 1831, 2047
BseRI GAGGAG 3 cut(s) 1091, 2535, 3257
BseYI CCCAGC 2 cut(s) 1189, 1198
BsgI GTGCAG 2 cut(s) 1169, 3371
Bsh1236I CGCG 1 cut(s) 2732
BshFI GGCC 9 cut(s) 61, 1274, 1341, 2162, 2210, 2329, 2482, 2729, 2795
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 4 cut(s) 1218, 2117, 2278, 3109
BsiHKCI CYCGRG 1 cut(s) 3119
BsiSI CCGG 6 cut(s) 686, 833, 1275, 1438, 1995, 2437
BslFI GGGAC 2 cut(s) 2333, 2714
BslI CCNNNNNNNGG 6 cut(s) 404, 839, 911, 2253, 2930, 3049
BsmAI GTCTC 4 cut(s) 1021, 1669, 2778, 2865
BsmFI GGGAC 2 cut(s) 2333, 2714
BsmI GAATGC 1 cut(s) 1257
BsnI GGCC 9 cut(s) 61, 1274, 1341, 2162, 2210, 2329, 2482, 2729, 2795
BsoBI CYCGRG 1 cut(s) 3119
Bsp1286I GDGCHC 6 cut(s) 179, 1218, 2117, 2278, 2829, 3109
Bsp1720I GCTNAGC 2 cut(s) 776, 1194
BspACI CCGC 1 cut(s) 2730
BspANI GGCC 9 cut(s) 61, 1274, 1341, 2162, 2210, 2329, 2482, 2729, 2795
BspDI ATCGAT 1 cut(s) 81
BspFNI CGCG 1 cut(s) 2732
BspHI TCATGA 2 cut(s) 2125, 2671
BspLI GGNNCC 6 cut(s) 218, 1398, 1493, 2563, 2609, 2728
BspMAI CTGCAG 4 cut(s) 789, 995, 1158, 3141
BspMI ACCTGC 2 cut(s) 706, 2891
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 4 cut(s) 1180, 1468, 1828, 3014
BspTI CTTAAG 1 cut(s) 1591
BsrFI RCCGGY 1 cut(s) 1274
BsrI ACTGG 8 cut(s) 218, 295, 388, 532, 1275, 1327, 1831, 2047
BssAI RCCGGY 1 cut(s) 1274
BssECI CCNNGG 3 cut(s) 1606, 2330, 2557
BssNAI GTATAC 1 cut(s) 2968
BssT1I CCWWGG 3 cut(s) 1606, 2330, 2557
Bst1107I GTATAC 1 cut(s) 2968
Bst4CI ACNGT 8 cut(s) 449, 848, 1222, 1532, 1706, 1848, 2076, 2896
Bst6I CTCTTC 3 cut(s) 101, 1774, 3062
BstAFI CTTAAG 1 cut(s) 1591
BstAPI GCANNNNNTGC 1 cut(s) 119
BstC8I GCNNGC 6 cut(s) 309, 482, 562, 729, 782, 2850
BstEII GGTNACC 1 cut(s) 602
BstF5I GGATG 6 cut(s) 694, 1666, 1731, 1836, 2423, 3361
BstFNI CGCG 1 cut(s) 2732
BstHHI GCGC 2 cut(s) 560, 2734
BstMAI GTCTC 4 cut(s) 1021, 1669, 2778, 2865
BstNSI RCATGY 3 cut(s) 673, 784, 2644
BstPAI GACNNNNGTC 1 cut(s) 2077
BstPI GGTNACC 1 cut(s) 602
BstSCI CCNGG 3 cut(s) 684, 1994, 2436
BstSFI CTRYAG 5 cut(s) 785, 991, 1154, 2772, 3137
BstUI CGCG 1 cut(s) 2732
BstV2I GAAGAC 5 cut(s) 94, 1010, 1280, 2463, 2558
BstX2I RGATCY 2 cut(s) 1300, 1473
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 2 cut(s) 1300, 1473
BstZ17I GTATAC 1 cut(s) 2968
Bsu15I ATCGAT 1 cut(s) 81
Bsu36I CCTNAGG 1 cut(s) 975
BsuI GTATCC 1 cut(s) 2927
BsuRI GGCC 9 cut(s) 61, 1274, 1341, 2162, 2210, 2329, 2482, 2729, 2795
BsuTUI ATCGAT 1 cut(s) 81
BtgZI GCGATG 2 cut(s) 2202, 2976
BtsCI GGATG 6 cut(s) 694, 1666, 1731, 1836, 2423, 3361
BtsI GCAGTG 1 cut(s) 3331
BtsIMutI CAGTG 5 cut(s) 279, 1320, 1471, 2040, 3331
BveI ACCTGC 2 cut(s) 706, 2891
Cac8I GCNNGC 6 cut(s) 309, 482, 562, 729, 782, 2850
CaiI CAGNNNCTG 3 cut(s) 502, 793, 2492
CciI TCATGA 2 cut(s) 2125, 2671
CfoI GCGC 2 cut(s) 560, 2734
Cfr10I RCCGGY 1 cut(s) 1274
Cfr13I GGNCC 6 cut(s) 246, 499, 1397, 2727, 2813, 2883
ClaI ATCGAT 1 cut(s) 81
CseI GACGC 1 cut(s) 799
Csp6I GTAC 8 cut(s) 536, 1425, 1547, 2151, 2638, 2720, 3086, 3381
CspCI CAANNNNNGTGG 2 cut(s) 891, 926
CviQI GTAC 8 cut(s) 536, 1425, 1547, 2151, 2638, 2720, 3086, 3381
DraIII CACNNNGTG 1 cut(s) 3049
EaeI YGGCCR 4 cut(s) 1272, 2160, 2208, 2480
Eam1104I CTCTTC 3 cut(s) 101, 1774, 3062
EarI CTCTTC 3 cut(s) 101, 1774, 3062
Ecl136II GAGCTC 1 cut(s) 1216
Eco130I CCWWGG 3 cut(s) 1606, 2330, 2557
Eco24I GRGCYC 3 cut(s) 179, 1218, 2829
Eco32I GATATC 1 cut(s) 2711
Eco47I GGWCC 5 cut(s) 246, 499, 1397, 2813, 2883
Eco53kI GAGCTC 1 cut(s) 1216
Eco57I CTGAAG 4 cut(s) 155, 207, 479, 587
Eco81I CCTNAGG 1 cut(s) 975
Eco88I CYCGRG 1 cut(s) 3119
Eco91I GGTNACC 1 cut(s) 602
EcoICRI GAGCTC 1 cut(s) 1216
EcoO109I RGGNCCY 1 cut(s) 499
EcoO65I GGTNACC 1 cut(s) 602
EcoRI GAATTC 1 cut(s) 3283
EcoRV GATATC 1 cut(s) 2711
EcoT14I CCWWGG 3 cut(s) 1606, 2330, 2557
EcoT38I GRGCYC 3 cut(s) 179, 1218, 2829
ErhI CCWWGG 3 cut(s) 1606, 2330, 2557
FalI AAGNNNNNCTT 2 cut(s) 1358, 1390
FaqI GGGAC 2 cut(s) 2333, 2714
FbaI TGATCA 3 cut(s) 3091, 3295, 3315
FblI GTMKAC 1 cut(s) 2967
FokI GGATG 6 cut(s) 701, 1673, 1718, 1843, 2430, 3348
FriOI GRGCYC 3 cut(s) 179, 1218, 2829
GlaI GCGC 2 cut(s) 559, 2733
GsaI CCCAGC 2 cut(s) 1193, 1202
GsuI CTGGAG 2 cut(s) 675, 950
HaeIII GGCC 9 cut(s) 61, 1274, 1341, 2162, 2210, 2329, 2482, 2729, 2795
HapII CCGG 6 cut(s) 686, 833, 1275, 1438, 1995, 2437
HgaI GACGC 1 cut(s) 799
HhaI GCGC 2 cut(s) 560, 2734
Hin6I GCGC 2 cut(s) 558, 2732
HinP1I GCGC 2 cut(s) 558, 2732
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HindIII AAGCTT 6 cut(s) 917, 997, 1367, 1820, 1967, 3097
HpaII CCGG 6 cut(s) 686, 833, 1275, 1438, 1995, 2437
HphI GGTGA 8 cut(s) 35, 64, 255, 387, 563, 833, 1988, 2560
Hpy166II GTNNAC 9 cut(s) 72, 263, 862, 896, 1709, 2434, 2666, 2779, 2968
Hpy8I GTNNAC 9 cut(s) 72, 263, 862, 896, 1709, 2434, 2666, 2779, 2968
HpyCH4III ACNGT 8 cut(s) 449, 848, 1222, 1532, 1706, 1848, 2076, 2896
HpyCH4IV ACGT 1 cut(s) 116
HpySE526I ACGT 1 cut(s) 116
HspAI GCGC 2 cut(s) 558, 2732
Ksp22I TGATCA 3 cut(s) 3091, 3295, 3315
LmnI GCTCC 7 cut(s) 544, 1078, 1213, 1631, 2567, 2652, 2926
LweI GCATC 2 cut(s) 2225, 2408
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 8 cut(s) 277, 602, 627, 1091, 1325, 1595, 1700, 2684
MfeI CAATTG 1 cut(s) 3015
MflI RGATCY 2 cut(s) 1300, 1473
MhlI GDGCHC 6 cut(s) 179, 1218, 2117, 2278, 2829, 3109
MlsI TGGCCA 3 cut(s) 2162, 2210, 2482
MluNI TGGCCA 3 cut(s) 2162, 2210, 2482
MlyI GAGTC 4 cut(s) 1294, 2315, 2753, 3033
MmeI TCCRAC 6 cut(s) 234, 468, 2585, 2913, 3047, 3398
Mox20I TGGCCA 3 cut(s) 2162, 2210, 2482
MroXI GAANNNNTTC 2 cut(s) 492, 645
MscI TGGCCA 3 cut(s) 2162, 2210, 2482
MseI TTAA 8 cut(s) 509, 1361, 1592, 1722, 1751, 2202, 2790, 3168
MslI CAYNNNNRTG 1 cut(s) 3160
Msp20I TGGCCA 3 cut(s) 2162, 2210, 2482
MspA1I CMGCKG 4 cut(s) 1153, 1193, 1351, 2543
MspCI CTTAAG 1 cut(s) 1591
MspI CCGG 6 cut(s) 686, 833, 1275, 1438, 1995, 2437
MspR9I CCNGG 3 cut(s) 686, 1996, 2438
MunI CAATTG 1 cut(s) 3015
Mva1269I GAATGC 1 cut(s) 1257
MvnI CGCG 1 cut(s) 2732
NciI CCSGG 3 cut(s) 686, 1996, 2438
NheI GCTAGC 1 cut(s) 307
NlaIV GGNNCC 6 cut(s) 218, 1398, 1493, 2563, 2609, 2728
NmuCI GTSAC 1 cut(s) 277
NspI RCATGY 3 cut(s) 673, 784, 2644
PaeI GCATGC 1 cut(s) 784
PagI TCATGA 2 cut(s) 2125, 2671
PaqCI CACCTGC 1 cut(s) 706
PciI ACATGT 1 cut(s) 669
PctI GAATGC 1 cut(s) 1257
PdmI GAANNNNTTC 2 cut(s) 492, 645
PfeI GAWTC 7 cut(s) 571, 826, 1461, 1672, 1690, 2303, 2531
PflFI GACNNNGTC 1 cut(s) 793
PflMI CCANNNNNTGG 1 cut(s) 3049
PfoI TCCNGGA 1 cut(s) 684
PleI GAGTC 4 cut(s) 1293, 2315, 2753, 3033
PpsI GAGTC 4 cut(s) 1293, 2315, 2753, 3033
PpuMI RGGWCCY 1 cut(s) 499
PscI ACATGT 1 cut(s) 669
PshAI GACNNNNGTC 1 cut(s) 2077
PsiI TTATAA 1 cut(s) 2003
Psp124BI GAGCTC 1 cut(s) 1218
Psp5II RGGWCCY 1 cut(s) 499
PspEI GGTNACC 1 cut(s) 602
PspFI CCCAGC 2 cut(s) 1189, 1198
PspN4I GGNNCC 6 cut(s) 218, 1398, 1493, 2563, 2609, 2728
PspPI GGNCC 6 cut(s) 246, 499, 1397, 2727, 2813, 2883
PspPPI RGGWCCY 1 cut(s) 499
PsrI GAACNNNNNNTAC 2 cut(s) 2012, 2044
PstI CTGCAG 4 cut(s) 789, 995, 1158, 3141
PstNI CAGNNNCTG 3 cut(s) 502, 793, 2492
PsuI RGATCY 2 cut(s) 1300, 1473
PsyI GACNNNGTC 1 cut(s) 793
PvuII CAGCTG 4 cut(s) 1153, 1193, 1351, 2543
RsaI GTAC 8 cut(s) 537, 1426, 1548, 2152, 2639, 2721, 3087, 3382
RsaNI GTAC 8 cut(s) 536, 1425, 1547, 2151, 2638, 2720, 3086, 3381
RseI CAYNNNNRTG 1 cut(s) 3160
SacI GAGCTC 1 cut(s) 1218
SaqAI TTAA 8 cut(s) 509, 1361, 1592, 1722, 1751, 2202, 2790, 3168
Sau96I GGNCC 6 cut(s) 246, 499, 1397, 2727, 2813, 2883
ScaI AGTACT 1 cut(s) 2721
SchI GAGTC 4 cut(s) 1294, 2315, 2753, 3033
ScrFI CCNGG 3 cut(s) 686, 1996, 2438
SduI GDGCHC 6 cut(s) 179, 1218, 2117, 2278, 2829, 3109
SfaNI GCATC 2 cut(s) 2225, 2408
SfcI CTRYAG 5 cut(s) 785, 991, 1154, 2772, 3137
SinI GGWCC 5 cut(s) 246, 499, 1397, 2813, 2883
SmiMI CAYNNNNRTG 1 cut(s) 3160
SmlI CTYRAG 2 cut(s) 1073, 1591
SmoI CTYRAG 2 cut(s) 1073, 1591
SphI GCATGC 1 cut(s) 784
SsiI CCGC 1 cut(s) 2730
SstI GAGCTC 1 cut(s) 1218
StyD4I CCNGG 3 cut(s) 684, 1994, 2436
StyI CCWWGG 3 cut(s) 1606, 2330, 2557
TaaI ACNGT 8 cut(s) 449, 848, 1222, 1532, 1706, 1848, 2076, 2896
TaiI ACGT 1 cut(s) 119
TaqI TCGA 9 cut(s) 56, 81, 649, 797, 1248, 1409, 2071, 2272, 3281
TaqII GACCGA 1 cut(s) 2871
TatI WGTACW 3 cut(s) 1424, 2637, 2719
TauI GCSGC 1 cut(s) 2732
TfiI GAWTC 7 cut(s) 571, 826, 1461, 1672, 1690, 2303, 2531
Tru1I TTAA 8 cut(s) 509, 1361, 1592, 1722, 1751, 2202, 2790, 3168
Tru9I TTAA 8 cut(s) 509, 1361, 1592, 1722, 1751, 2202, 2790, 3168
TscAI CASTG 5 cut(s) 286, 1327, 1471, 2047, 3331
TseFI GTSAC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 277
TspGWI ACGGA 1 cut(s) 1727
TspRI CASTG 5 cut(s) 286, 1327, 1471, 2047, 3331
Tth111I GACNNNGTC 1 cut(s) 793
Van91I CCANNNNNTGG 1 cut(s) 3049
Vha464I CTTAAG 1 cut(s) 1591
VpaK11BI GGWCC 5 cut(s) 246, 499, 1397, 2813, 2883
XapI RAATTY 4 cut(s) 328, 1024, 1357, 3283
XbaI TCTAGA 3 cut(s) 1297, 2117, 2168
XceI RCATGY 3 cut(s) 673, 784, 2644
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 2967
XmnI GAANNNNTTC 2 cut(s) 492, 645
ZrmI AGTACT 1 cut(s) 2721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.