pycom10g14630
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013816
Physical Location & Seq
Forward (+)
17998802 .. 18002662
3861 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g14630.1

Sequence Viewer

Length: 3489 bp
ATGCCAATGCTGAGCAGCAAGCACTCCTCTCTCCTCTCCTACAACCACCACCAAATGCCGAGGCAATCCCTCACCACTCGCTGCTACCACCACCACCACCACCACTTGCTCTCTCCTCTCCTCGTCTTCCTCTTGCTATCCACACTTCTACCGCTTTCAGCTACAAACATTGAGGTTGTCACACTACATTCATGGCTTCAAACCTCTACCTTGCCCCCGCCGTCCTCATCGGATTTCTCCAACTGGAACCCTAAAGACCCCAACCCTTGTAGCTGGTCCTACATAACCTGTTCTCCTCAAAACTTTGTCACCGAAATCAATATCCAATCCCTTGAGCTAGCCCTTCCCTTTCCTTCCAATCTCTCCTCCCTTGCCTCTCTCCAAAAGCTCATCATTTCTGGTGCTAACCTCACCGGGACACTCTCACCTGATATCGGATACTGCAATGCACTTACTGTCATTGATGTTAGCTCAAACGGTCTTGTGGGAGAATTTCCTTCAACCATTGGAAAGCTGCAAAATCTTCAGGACTTGATTTTAAATTCTAATCAACTCACTGGGCAGATACCAAAAGAGCTTGGAGATTGCACCAGCCTGAAGAATCTCCACGTTTTTGACAACTATCTCTCCGGGAGTATTCCTGCCGAGCTTGGAAAACTAGTAAACTTGGAAGTTATTCGTGCCGGTGGGAACAAAGACATTTCAGGAAAAATCCCCGATGAGCTTGGAAACTGCAAGAACTTGCTGGTGCTTGGCCTTGCTGATACTAAAGTTTCCGGCTCCTTACCTGCTTCATTAGGTAAACTACGCATGCTGCAAACTCTGTCTGTTTATACCACAATGATTTCGGGTGAGATTCCGCCGGAGATTGGCAACTGTTCGGAGCTTGTGAACTTGTTTTTGTACGAGAATGATCTGTCCGGTTCGCTACCATCAGAGTTAGGTAAGCTTCAGAAATTGGAGAAGATGTTACTGTGGCAAAACAATCTGGATGGTAAAATTCCTGAGGAAATTGGTAACTGCAGAAGCTTGATGACCATAGATCTCTCTTTGAATTCTGTCTCTGGAAGCATACCACAATCATTCGGGAACCTCTCGAATCTCCAAGAGCTTATGCTTAGCAACAATAACATTTCTGGTTCAATCCCATCAGTTCTTTCCAATGCTACAAAACTCTTGCAACTTCAGCTCGACACTAATCAGATTTCGGGCTTAATCCCAACTCAGTTTGGAATGTTGCCAGAGCTGACAGTGTTTTTCGCTTGGCAGAACAAACTCGAAGGCAGCATTCCATCCGAATTGGCTAGCTGTAAGAGCCTTCAGGCTCTTGATCTCTCACACAATGCTCTCACCGGTAGCTTACCTCCTGGCCTATTTCAGCTCCAAAATTTAACCAAGCTTCTATTGATTTCTAATGAACTTTCCGGTTCCATACCTTCTGATATTGGAAATTGCAGCTCTCTTATCCGTCTCCGCCTTGTAAACAACAGAATCAGTGGAGAGATCCCAAAAGAAATTGGGTTTCTCGACAACCTTAGCTTCCTTGATCTGTCCGAAAACAATCTTGTTGGTCCGCTGCCTGATGAAATTGGAAAATGCAGTTCACTGCAGCTGCTGAACTTAAGTAACAATACCCTTGGAGGTACATTGCCTAGCCTTTTCTCTTCCCTCACACGGCTTGAGGTACTGGATGTCTCTGTGAATCAAATTTCGGGTCAGATTCCCGAGAGTTATGGCAAGCTTGCTTCACTTAACCGGCTCATCCTCAGCAGGAACTCCCTCTCTGGACTGATCCCTTCTTCCCTTGGTCATTGTTCAAGCCTTCAGCTACTTGATCTTAGCAGCAACAAGCTCAGTGGTGCAATCCCCGAGGACTTGTTTGAGATTGAAGCTCTTGACATTGCTCTAAACCTGAGTTTCAATGCCTTATCCGGTGTAATCCCACCGCAGATCTCTGCTCTGAACAAGCTGTTCATACTAGACCTTTCACACAACAAGCTTGAAGGTGACTTGCTGGTGTTATCCGGGCTTGCAAATCTTGTTTCCTTGAACATCTCATATAACAATTTTACCGGCTATCTCCCCGATGAAAAGCTCTTTCGACAGTTGTCACCAACGGACCTGGCAGGAAACAAAGGGTTGTGCTCTAAAGGCCATGACCAATGTTTCATCAGCAATGGGACCACAGTGAGCATGACAAATAGCGGCCGTTTCAGGAGGTCATGGAGGCTTAAGTTAGCCATTGGATTGTTGATTGCCTTGACCATTGCCTTAGCAATTTTCGGGGCTGTTGCAGTGTATCGAACACGAAAGATGATGGGAGACGAAAACGATTCTGAGATGGGAGGGGACTCGTGGCCTTGGCAGTTTACTCCCTTCCAGAAGGTAAATTTTTCGGTGGATCAAGTACTAAAATGTTTGGTGGAAACCAATGTAATAGGAAAGGGATGTTCAGGAGTTGTTTACCGGGCGGAAATGGAGAACGAGGACATTGCAGTAAAGAAGCTCTGGCCAACAAAGATCGCTACTAGATATGACTGTCAACGTGACAGGTTTGGAATCAATGCAGGTGTTCGCGATTCCTTTTCAGCTGAGGTGAAGACCCTTGGCTCAATTCGCCACAAGAACATTGTTAGGTTCTTGGGTTGCTGTTGGAATCGAAACACGAGACTGCTAATGTATGAGTACATGTCGAATGGGAGCTTGGGCGGTTTACTTCACGAAAGAAGTGGTAACTGCTTGGAATGGGAATTGAGATATCGGATTGTGCTGGGAGCGGCACAAGGCTTGGCCTACTTGCATCACGATTGCGTTCCTCCGATTGTTCACAGGGACATCAAGGCCAATAACATCCTCATTGGTCCGGACTTTGAGCCCTGCATTGCTGATTTCGGGCTTGCCAAGCTTGTTGACGAAGGAGATTTTGCTCGATCCTCCAACACAGTTGCTGGTTCTTATGGCTACATTGCTCCTGATGGATACATGATGAAGATAACAGAGAAAAGTGACGTATACAGCTACGGTGTAGTTGTGTTAGAAGTACTAACAGGGAAACAACCGATTGATCCAACCATACCAGACGGGTTGCACATTGTAGATTGGGTTAGGCAGAAGAGAGGGGGAGTTGAAGTCCTCGATCAAAGCCTACTTGCCCGGCCAGAGTCTGAAATTGAAGAAATGCTGCAGACTCTAGGTGTGGCGTTGCTCTGCATAAACTCAAACCCAGATGACAGACCAACCATGAAAGACGTAGCGGCAATGCTCAAAGAAATAAGGCAGGAGAGGGAGGAGTGCATGAAAGCTAACATGCTTCGAAACGGATCTTCTGAAAATGGTGGACAAGAAAATAACATAACCTGCAGTGGAGGCAGCTCATCGGCTATGGCGGTGCAGCAACATTCGTACCCTTCGAGTACCAGCGCAAGTTTCTCAGCATCCTCATTGCTAAACACTTCTCCTCCTCCTAATGTCCGAAAAAGTTCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1163

Amino Acids

126.53

Weight (kDa)

5.68

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 120 - 227 4.5e-09 Leucine-rich repeat region
LRR_8 PF13855 151 - 209 2.1e-06 Leucine rich repeat
LRR_14 PF23598 241 - 326 6.1e-10 Leucine-rich repeat region
LRR_8 PF13855 319 - 378 3.4e-06 Leucine rich repeat
LRR_14 PF23598 333 - 419 3.7e-06 Leucine-rich repeat region
LRR_8 PF13855 342 - 402 2.1e-07 Leucine rich repeat
LRR_14 PF23598 429 - 517 9.6e-07 Leucine-rich repeat region
LRR_14 PF23598 491 - 704 1.5e-13 Leucine-rich repeat region
LRR_8 PF13855 535 - 594 3.3e-06 Leucine rich repeat
LRR_8 PF13855 559 - 618 1.3e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 810 - 1087 1.9e-37 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 810 - 1085 4.3e-37 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48940
fragaria_vesca FvH4_2g19620
malus_domestica MD10G1169800.v1.1
prunus_persica Prupe.8G207600_v2.0.a1
pyrus_communis pycom10g14630
rosa_chinensis RchiOBHm_Chr6g0285351
rosa_laevigata RLG00000012651
rosa_multiflora Rmu_co8152086.1_g000001 Rmu_co8221716.1_g000001 Rmu_sc0012287.1_g000004
rosa_roxburghii Rroxscaffold_7G00183330
rosa_rugosa Rorug06G0173900
rosa_samantha Rh6AG284500 Rh6BG285700 Rh6CG286800 Rh6DG279800
rosa_wichuraiana Rw6G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2558
Acc36I ACCTGC 3 cut(s) 796, 2558, 3365
AccBSI CCGCTC 1 cut(s) 2777
AccI GTMKAC 1 cut(s) 3012
AccII CGCG 1 cut(s) 2577
AccIII TCCGGA 1 cut(s) 2863
AclWI GGATC 6 cut(s) 1498, 1786, 2409, 2925, 3059, 3328
AcoI YGGCCR 3 cut(s) 2206, 2510, 3155
AcsI RAATTY 7 cut(s) 491, 541, 999, 1054, 1387, 1707, 2389
AcuI CTGAAG 6 cut(s) 509, 617, 935, 1169, 1304, 1808
AfaI GTAC 8 cut(s) 905, 1645, 1686, 2409, 2687, 3042, 3404, 3415
AfiI CCNNNNNNNGG 3 cut(s) 434, 869, 1674
AflII CTTAAG 2 cut(s) 1621, 2231
AflIII ACRYGT 1 cut(s) 2688
AgeI ACCGGT 1 cut(s) 1352
AhlI ACTAGT 1 cut(s) 658
AjnI CCWGG 2 cut(s) 1366, 2121
AjuI GAANNNNNNNTTGG 2 cut(s) 2687, 2719
Alw21I GWGCWC 1 cut(s) 2147
Alw26I GTCTC 5 cut(s) 1066, 1475, 1699, 2316, 2662
AlwI GGATC 6 cut(s) 1498, 1786, 2409, 2925, 3059, 3328
AlwNI CAGNNNCTG 3 cut(s) 1615, 2948, 3164
Ama87I CYCGRG 2 cut(s) 1724, 1868
Aor13HI TCCGGA 1 cut(s) 2863
AoxI GGCC 9 cut(s) 754, 1369, 2152, 2206, 2357, 2510, 2790, 2841, 3155
ApoI RAATTY 7 cut(s) 491, 541, 999, 1054, 1387, 1707, 2389
ArsI GACNNNNNNTTYG 2 cut(s) 1699, 1731
AsiGI ACCGGT 1 cut(s) 1352
Asp700I GAANNNNTTC 2 cut(s) 675, 3478
AspLEI GCGC 1 cut(s) 3422
AspS9I GGNCC 5 cut(s) 276, 1571, 2119, 2181, 2861
AsuC2I CCSGG 5 cut(s) 415, 631, 2026, 2468, 3154
AsuHPI GGTGA 9 cut(s) 64, 301, 403, 417, 863, 1342, 2018, 2103, 2608
AsuII TTCGAA 1 cut(s) 3313
AsuNHI GCTAGC 2 cut(s) 337, 1304
AvaI CYCGRG 2 cut(s) 1724, 1868
AvaII GGWCC 5 cut(s) 276, 1571, 2119, 2181, 2861
AxyI CCTNAGG 1 cut(s) 1005
BaeI ACNNNNGTAYC 4 cut(s) 756, 789, 3386, 3419
BalI TGGCCA 1 cut(s) 2512
BanII GRGCYC 1 cut(s) 2877
BauI CACGAG 2 cut(s) 2353, 2665
BbsI GAAGAC 2 cut(s) 118, 2606
Bbv12I GWGCWC 1 cut(s) 2147
BbvCI CCTCAGC 2 cut(s) 1766, 2592
BccI CCATC 7 cut(s) 940, 986, 1156, 1300, 2311, 2335, 2969
BceAI ACGGC 3 cut(s) 205, 1691, 2193
BcgI CGANNNNNNTGC 2 cut(s) 2474, 2508
BciT130I CCWGG 2 cut(s) 1368, 2123
BciVI GTATCC 2 cut(s) 431, 2972
BcnI CCSGG 5 cut(s) 415, 631, 2026, 2468, 3154
BcoDI GTCTC 5 cut(s) 1066, 1475, 1699, 2316, 2662
BcuI ACTAGT 1 cut(s) 658
BfaI CTAG 7 cut(s) 338, 659, 1305, 1653, 1979, 2529, 3191
BfmI CTRYAG 4 cut(s) 1021, 1607, 3182, 3358
BfrI CTTAAG 2 cut(s) 1621, 2231
BfuAI ACCTGC 3 cut(s) 796, 2558, 3365
BfuI GTATCC 2 cut(s) 431, 2972
BglII AGATCT 2 cut(s) 1042, 1950
BlpI GCTNAGC 2 cut(s) 11, 1118
BmcAI AGTACT 2 cut(s) 2409, 3042
Bme1390I CCNGG 7 cut(s) 415, 631, 1368, 2026, 2123, 2468, 3154
Bme18I GGWCC 5 cut(s) 276, 1571, 2119, 2181, 2861
BmeT110I CYCGRG 2 cut(s) 1724, 1868
BmgT120I GGNCC 5 cut(s) 276, 1571, 2119, 2181, 2861
BmiI GGNNCC 5 cut(s) 248, 781, 1091, 1429, 2182
BmrFI CCNGG 7 cut(s) 415, 631, 1368, 2026, 2123, 2468, 3154
BmrI ACTGGG 1 cut(s) 567
BmsI GCATC 2 cut(s) 2809, 3443
BmtI GCTAGC 2 cut(s) 341, 1308
BmuI ACTGGG 1 cut(s) 567
BoxI GACNNNNGTC 1 cut(s) 2107
BpiI GAAGAC 2 cut(s) 118, 2606
Bpu10I CCTNAGC 4 cut(s) 1535, 1766, 2272, 2592
Bpu1102I GCTNAGC 2 cut(s) 11, 1118
Bpu14I TTCGAA 1 cut(s) 3313
BpuEI CTTGAG 2 cut(s) 353, 1700
BpuMI CCSGG 5 cut(s) 415, 631, 2026, 2468, 3154
BsaJI CCNNGG 6 cut(s) 59, 1636, 1804, 1869, 2360, 2605
BsaWI WCCGGW 5 cut(s) 920, 1352, 1424, 1931, 2863
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 869, 1674
Bse118I RCCGGY 4 cut(s) 683, 1352, 1755, 2072
Bse1I ACTGG 3 cut(s) 248, 562, 1692
Bse21I CCTNAGG 1 cut(s) 1005
BseAI TCCGGA 1 cut(s) 2863
BseBI CCWGG 2 cut(s) 1368, 2123
BseDI CCNNGG 6 cut(s) 59, 1636, 1804, 1869, 2360, 2605
BseGI GGATG 7 cut(s) 997, 1292, 1696, 1761, 2453, 2850, 3434
BseLI CCNNNNNNNGG 3 cut(s) 434, 869, 1674
BseMII CTCAG 8 cut(s) 996, 1238, 1780, 1867, 1904, 2328, 2583, 3444
BseNI ACTGG 3 cut(s) 248, 562, 1692
BseRI GAGGAG 9 cut(s) 16, 23, 105, 110, 285, 355, 3302, 3447, 3450
BseX3I CGGCCG 1 cut(s) 2206
BseYI CCCAGC 1 cut(s) 2770
BsgI GTGCAG 1 cut(s) 3410
Bsh1236I CGCG 1 cut(s) 2577
Bsh1285I CGRYCG 1 cut(s) 2209
BshFI GGCC 9 cut(s) 756, 1371, 2154, 2208, 2359, 2512, 2792, 2843, 3157
BshTI ACCGGT 1 cut(s) 1352
BsiEI CGRYCG 1 cut(s) 2209
BsiHKAI GWGCWC 1 cut(s) 2147
BsiHKCI CYCGRG 2 cut(s) 1724, 1868
BslFI GGGAC 4 cut(s) 430, 2194, 2363, 2846
BslI CCNNNNNNNGG 3 cut(s) 434, 869, 1674
BsmAI GTCTC 5 cut(s) 1066, 1475, 1699, 2316, 2662
BsmBI CGTCTC 2 cut(s) 1475, 2316
BsmFI GGGAC 4 cut(s) 430, 2194, 2363, 2846
BsmI GAATGC 1 cut(s) 1287
BsnI GGCC 9 cut(s) 756, 1371, 2154, 2208, 2359, 2512, 2792, 2843, 3157
BsoBI CYCGRG 2 cut(s) 1724, 1868
Bsp119I TTCGAA 1 cut(s) 3313
Bsp1286I GDGCHC 2 cut(s) 2147, 2877
Bsp13I TCCGGA 1 cut(s) 2863
Bsp1720I GCTNAGC 2 cut(s) 11, 1118
Bsp68I TCGCGA 1 cut(s) 2577
BspANI GGCC 9 cut(s) 756, 1371, 2154, 2208, 2359, 2512, 2792, 2843, 3157
BspCNI CTCAG 8 cut(s) 997, 1237, 1779, 1866, 1905, 2329, 2584, 3443
BspEI TCCGGA 1 cut(s) 2863
BspFNI CGCG 1 cut(s) 2577
BspLI GGNNCC 5 cut(s) 248, 781, 1091, 1429, 2182
BspMAI CTGCAG 4 cut(s) 1025, 1611, 3186, 3362
BspMI ACCTGC 3 cut(s) 796, 2558, 3365
BspOI GCTAGC 2 cut(s) 341, 1308
BspPI GGATC 6 cut(s) 1498, 1786, 2409, 2925, 3059, 3328
BspT104I TTCGAA 1 cut(s) 3313
BspTI CTTAAG 2 cut(s) 1621, 2231
BsrBI CCGCTC 1 cut(s) 2777
BsrFI RCCGGY 4 cut(s) 683, 1352, 1755, 2072
BsrI ACTGG 3 cut(s) 248, 562, 1692
BssAI RCCGGY 4 cut(s) 683, 1352, 1755, 2072
BssECI CCNNGG 6 cut(s) 59, 1636, 1804, 1869, 2360, 2605
BssNAI GTATAC 1 cut(s) 3013
BssSI CACGAG 2 cut(s) 2353, 2665
BssT1I CCWWGG 4 cut(s) 1636, 1804, 2360, 2605
Bst1107I GTATAC 1 cut(s) 3013
Bst2BI CACGAG 2 cut(s) 2353, 2665
Bst2UI CCWGG 2 cut(s) 1368, 2123
Bst6I CTCTTC 2 cut(s) 1669, 3107
BstAFI CTTAAG 2 cut(s) 1621, 2231
BstBI TTCGAA 1 cut(s) 3313
BstC8I GCNNGC 8 cut(s) 20, 339, 812, 1306, 1739, 1743, 2031, 2898
BstF5I GGATG 7 cut(s) 997, 1292, 1696, 1761, 2453, 2850, 3434
BstFNI CGCG 1 cut(s) 2577
BstHHI GCGC 1 cut(s) 3422
BstMAI GTCTC 5 cut(s) 1066, 1475, 1699, 2316, 2662
BstMCI CGRYCG 1 cut(s) 2209
BstMWI GCNNNNNNNGC 6 cut(s) 1186, 1314, 2151, 2616, 2902, 3366
BstNI CCWGG 2 cut(s) 1368, 2123
BstNSI RCATGY 3 cut(s) 814, 2692, 3310
BstPAI GACNNNNGTC 1 cut(s) 2107
BstSCI CCNGG 7 cut(s) 413, 629, 1366, 2024, 2121, 2466, 3152
BstSFI CTRYAG 4 cut(s) 1021, 1607, 3182, 3358
BstUI CGCG 1 cut(s) 2577
BstV2I GAAGAC 2 cut(s) 118, 2606
BstX2I RGATCY 4 cut(s) 1042, 1503, 1950, 3320
BstYI RGATCY 4 cut(s) 1042, 1503, 1950, 3320
BstZ17I GTATAC 1 cut(s) 3013
BstZI CGGCCG 1 cut(s) 2206
Bsu36I CCTNAGG 1 cut(s) 1005
BsuI GTATCC 2 cut(s) 431, 2972
BsuRI GGCC 9 cut(s) 756, 1371, 2154, 2208, 2359, 2512, 2792, 2843, 3157
BtsCI GGATG 7 cut(s) 997, 1292, 1696, 1761, 2453, 2850, 3434
BtsI GCAGTG 3 cut(s) 1604, 2301, 3367
BtsIMutI CAGTG 8 cut(s) 555, 1257, 1501, 1604, 1861, 2193, 2301, 3367
BtuMI TCGCGA 1 cut(s) 2577
BveI ACCTGC 3 cut(s) 796, 2558, 3365
Cac8I GCNNGC 8 cut(s) 20, 339, 812, 1306, 1739, 1743, 2031, 2898
CaiI CAGNNNCTG 3 cut(s) 1615, 2948, 3164
CfoI GCGC 1 cut(s) 3422
Cfr10I RCCGGY 4 cut(s) 683, 1352, 1755, 2072
Cfr13I GGNCC 5 cut(s) 276, 1571, 2119, 2181, 2861
Csp6I GTAC 8 cut(s) 904, 1644, 1685, 2408, 2686, 3041, 3403, 3414
CspAI ACCGGT 1 cut(s) 1352
CspCI CAANNNNNGTGG 2 cut(s) 1837, 1872
CviQI GTAC 8 cut(s) 904, 1644, 1685, 2408, 2686, 3041, 3403, 3414
DraI TTTAAA 1 cut(s) 540
EaeI YGGCCR 3 cut(s) 2206, 2510, 3155
EagI CGGCCG 1 cut(s) 2206
Eam1104I CTCTTC 2 cut(s) 1669, 3107
EarI CTCTTC 2 cut(s) 1669, 3107
EciI GGCGGA 3 cut(s) 849, 1463, 2486
EclXI CGGCCG 1 cut(s) 2206
Eco130I CCWWGG 4 cut(s) 1636, 1804, 2360, 2605
Eco24I GRGCYC 1 cut(s) 2877
Eco32I GATATC 2 cut(s) 433, 2759
Eco47I GGWCC 5 cut(s) 276, 1571, 2119, 2181, 2861
Eco52I CGGCCG 1 cut(s) 2206
Eco57I CTGAAG 6 cut(s) 509, 617, 935, 1169, 1304, 1808
Eco81I CCTNAGG 1 cut(s) 1005
Eco88I CYCGRG 2 cut(s) 1724, 1868
EcoRI GAATTC 1 cut(s) 1054
EcoRII CCWGG 2 cut(s) 1366, 2121
EcoRV GATATC 2 cut(s) 433, 2759
EcoT14I CCWWGG 4 cut(s) 1636, 1804, 2360, 2605
EcoT38I GRGCYC 1 cut(s) 2877
ErhI CCWWGG 4 cut(s) 1636, 1804, 2360, 2605
Esp3I CGTCTC 2 cut(s) 1475, 2316
FaqI GGGAC 4 cut(s) 430, 2194, 2363, 2846
FauI CCCGC 1 cut(s) 225
FblI GTMKAC 1 cut(s) 3012
FokI GGATG 7 cut(s) 1004, 1279, 1703, 1748, 2460, 2837, 3421
FriOI GRGCYC 1 cut(s) 2877
FspBI CTAG 7 cut(s) 338, 659, 1305, 1653, 1979, 2529, 3191
GlaI GCGC 1 cut(s) 3421
GsaI CCCAGC 1 cut(s) 2774
HaeIII GGCC 9 cut(s) 756, 1371, 2154, 2208, 2359, 2512, 2792, 2843, 3157
HhaI GCGC 1 cut(s) 3422
Hin6I GCGC 1 cut(s) 3420
HinP1I GCGC 1 cut(s) 3420
HincII GTYRAC 2 cut(s) 2543, 2911
HindII GTYRAC 2 cut(s) 2543, 2911
HindIII AAGCTT 6 cut(s) 947, 1027, 1397, 1739, 1997, 2903
HphI GGTGA 9 cut(s) 64, 301, 403, 417, 863, 1342, 2018, 2103, 2608
HpyCH4IV ACGT 4 cut(s) 609, 2545, 3009, 3249
HpyF10VI GCNNNNNNNGC 6 cut(s) 1186, 1314, 2151, 2616, 2902, 3366
HpySE526I ACGT 4 cut(s) 609, 2545, 3009, 3249
HspAI GCGC 1 cut(s) 3420
Kpn2I TCCGGA 1 cut(s) 2863
LmnI GCTCC 6 cut(s) 785, 883, 1386, 2700, 2774, 2974
LweI GCATC 2 cut(s) 2809, 3443
MaeI CTAG 7 cut(s) 338, 659, 1305, 1653, 1979, 2529, 3191
MaeII ACGT 4 cut(s) 609, 2545, 3009, 3249
MbiI CCGCTC 1 cut(s) 2777
MflI RGATCY 4 cut(s) 1042, 1503, 1950, 3320
MhlI GDGCHC 2 cut(s) 2147, 2877
MlsI TGGCCA 1 cut(s) 2512
MluNI TGGCCA 1 cut(s) 2512
MlyI GAGTC 3 cut(s) 2345, 3170, 3181
MmeI TCCRAC 4 cut(s) 264, 2633, 2961, 3092
Mox20I TGGCCA 1 cut(s) 2512
MroI TCCGGA 1 cut(s) 2863
MroXI GAANNNNTTC 2 cut(s) 675, 3478
MscI TGGCCA 1 cut(s) 2512
MseI TTAA 6 cut(s) 539, 1214, 1391, 1622, 1752, 2232
Msp20I TGGCCA 1 cut(s) 2512
MspA1I CMGCKG 3 cut(s) 1576, 1612, 2591
MspCI CTTAAG 2 cut(s) 1621, 2231
MspR9I CCNGG 7 cut(s) 415, 631, 1368, 2026, 2123, 2468, 3154
Mva1269I GAATGC 1 cut(s) 1287
MvaI CCWGG 2 cut(s) 1368, 2123
MvnI CGCG 1 cut(s) 2577
MwoI GCNNNNNNNGC 6 cut(s) 1186, 1314, 2151, 2616, 2902, 3366
NciI CCSGG 5 cut(s) 415, 631, 2026, 2468, 3154
NheI GCTAGC 2 cut(s) 337, 1304
NlaIV GGNNCC 5 cut(s) 248, 781, 1091, 1429, 2182
NmeAIII GCCGAG 2 cut(s) 84, 670
NmuCI GTSAC 6 cut(s) 178, 307, 2006, 2109, 2546, 3005
NruI TCGCGA 1 cut(s) 2577
NspI RCATGY 3 cut(s) 814, 2692, 3310
NspV TTCGAA 1 cut(s) 3313
PaeI GCATGC 1 cut(s) 814
PaqCI CACCTGC 1 cut(s) 2558
PciI ACATGT 1 cut(s) 2688
PcsI WCGNNNNNNNCGW 1 cut(s) 3407
PctI GAATGC 1 cut(s) 1287
PdmI GAANNNNTTC 2 cut(s) 675, 3478
PfoI TCCNGGA 1 cut(s) 629
PinAI ACCGGT 1 cut(s) 1352
PleI GAGTC 3 cut(s) 2345, 3169, 3181
PpsI GAGTC 3 cut(s) 2345, 3169, 3181
PscI ACATGT 1 cut(s) 2688
PshAI GACNNNNGTC 1 cut(s) 2107
Psp6I CCWGG 2 cut(s) 1366, 2121
PspFI CCCAGC 1 cut(s) 2770
PspGI CCWGG 2 cut(s) 1366, 2121
PspN4I GGNNCC 5 cut(s) 248, 781, 1091, 1429, 2182
PspPI GGNCC 5 cut(s) 276, 1571, 2119, 2181, 2861
PstI CTGCAG 4 cut(s) 1025, 1611, 3186, 3362
PstNI CAGNNNCTG 3 cut(s) 1615, 2948, 3164
PsuI RGATCY 4 cut(s) 1042, 1503, 1950, 3320
PvuII CAGCTG 2 cut(s) 1612, 2591
RruI TCGCGA 1 cut(s) 2577
RsaI GTAC 8 cut(s) 905, 1645, 1686, 2409, 2687, 3042, 3404, 3415
RsaNI GTAC 8 cut(s) 904, 1644, 1685, 2408, 2686, 3041, 3403, 3414
SaqAI TTAA 6 cut(s) 539, 1214, 1391, 1622, 1752, 2232
Sau96I GGNCC 5 cut(s) 276, 1571, 2119, 2181, 2861
ScaI AGTACT 2 cut(s) 2409, 3042
SchI GAGTC 3 cut(s) 2345, 3170, 3181
ScrFI CCNGG 7 cut(s) 415, 631, 1368, 2026, 2123, 2468, 3154
SduI GDGCHC 2 cut(s) 2147, 2877
SfaNI GCATC 2 cut(s) 2809, 3443
SfcI CTRYAG 4 cut(s) 1021, 1607, 3182, 3358
SfuI TTCGAA 1 cut(s) 3313
SinI GGWCC 5 cut(s) 276, 1571, 2119, 2181, 2861
SmlI CTYRAG 4 cut(s) 332, 1621, 1679, 2231
SmoI CTYRAG 4 cut(s) 332, 1621, 1679, 2231
SpeI ACTAGT 1 cut(s) 658
SphI GCATGC 1 cut(s) 814
SspMI CTAG 7 cut(s) 338, 659, 1305, 1653, 1979, 2529, 3191
StyD4I CCNGG 7 cut(s) 413, 629, 1366, 2024, 2121, 2466, 3152
StyI CCWWGG 4 cut(s) 1636, 1804, 2360, 2605
TaiI ACGT 4 cut(s) 612, 2548, 3012, 3252
TatI WGTACW 3 cut(s) 2407, 2685, 3040
TauI GCSGC 3 cut(s) 2208, 2780, 3257
Tru1I TTAA 6 cut(s) 539, 1214, 1391, 1622, 1752, 2232
Tru9I TTAA 6 cut(s) 539, 1214, 1391, 1622, 1752, 2232
TscAI CASTG 8 cut(s) 562, 1257, 1501, 1611, 1861, 2193, 2301, 3367
TseFI GTSAC 6 cut(s) 178, 307, 2006, 2109, 2546, 3005
Tsp45I GTSAC 6 cut(s) 178, 307, 2006, 2109, 2546, 3005
TspGWI ACGGA 3 cut(s) 1457, 2132, 3333
TspRI CASTG 8 cut(s) 562, 1257, 1501, 1611, 1861, 2193, 2301, 3367
Vha464I CTTAAG 2 cut(s) 1621, 2231
VpaK11BI GGWCC 5 cut(s) 276, 1571, 2119, 2181, 2861
XapI RAATTY 7 cut(s) 491, 541, 999, 1054, 1387, 1707, 2389
XceI RCATGY 3 cut(s) 814, 2692, 3310
XmiI GTMKAC 1 cut(s) 3012
XmnI GAANNNNTTC 2 cut(s) 675, 3478
XspI CTAG 7 cut(s) 338, 659, 1305, 1653, 1979, 2529, 3191
ZrmI AGTACT 2 cut(s) 2409, 3042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.