RchiOBHm_Chr6g0285351
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013816
Physical Location & Seq
Forward (+)
48651819 .. 48655454
3636 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25594

Sequence Viewer

Length: 3402 bp
ATGTCTAGGCCACTATCGTTCACCATCGATTTCTTCTTCCTCTTTGTCTTCACACTCGCAACGTCTGCTGCTTCTTTGGCTGCTGCAAATGTTGACGTTCTGACATTACATACATGGCTTCAAGGCTCTACTACCTCACCCTCTTCTTCTGATTTCTCCAACTGGAACCCTTCAGACCCCAACCCTTGTAATTGGTCCTACATTACTTGTTCTTCTCAAAACTTTGTCACTGAAATTAATATCCAGTTTATTGAGCTAGCCCTTCCCTTTCCTTCAAATTTTTCCTCCCTCGCATTTCTTCAAAAGCTCATCATTTCTGGTGCTAATCTTACTGGAACAATCTCACCTGATATTGGATATTGCAAATCGCTTCTGGTGCTTGACGTAAGTTCAAACAGTCTTGTGGGTAGTATTCCTTCAAGTATTGGCAGGCTTGAAAATCTTCAGGACCTGATCTTAAACTCTAACCAGCTTACTGGTCAAGTACCAATGGAGCTTGGTGATTGCGCCAGCCTGAAGAATCTTCTCATATTTGATAACTACCTAAGTGGTTATCTTCCTGCTACACTGGGAAAGTTACTAAACTTGGAAGTCATTCGAGCTGGAGGGAACAAAGACATGTCAGGAAATATCCCGGATGAGCTTGGAAACTGCAAGAACTTGCAGGTGTTGGGGCTTGCTGATACTAAAGTTTCTGGTTTGATACCGTCATCATTGGGTAAGCTCAGCATGCTGCAGACGCTGTCGATTTATACTACTATGCTATCGGGTGAGATTCCACCGGATATTGGCAACTGTTCAGAACTTGTCAACTTGTTCTTGTATGAGAATGACCTTTCTGGTTCACTACCACCAGAGTTGGGCAAGCTTCATAAGCTGGAGAAAATGTTATTGTGGCAAAACCACCTTGATGGGACAATTCCTGAGGAAATTGGGAACTGCAGAAGCTTGAAGACCATAGATGTCTCTCTAAATTCTTTTTCTGGAAGCATACCTCAGTCTTTCGGAAACCTCTCAAATCTTGAGGAGCTGATGCTTAGTAACAACAACATCTCTGGCTCAATTCCCTCAGTTCTTTCCAATGCTACAAAACTTGTGCAGCTGCAGCTTGATACTAATCAGATTTCAGGCTTGATCCCAGCTGAGATGGGAATGTTGAAGGAGCTCACAGTCTTTTTTGCTTGGCAGAACAAACTCGAAGGTAGTATTCCATCAGACTTGGCCGGTTGTGAGAGTCTTCAAGCTCTAGATCTCTCACACAATGCTCTCACTGGTAACATACCTTCTGGCCTATTTCAGCTGAAAAATTTAACCAAGCTTCTCTTGATTTCTAATGACATTTCGGGTCCAATACCTTCGGTGCTTGGAAATTGTACTTCTCTTATCCGGCTAAGGCTTGTGAATAACAGAATCAGTGGGGAGATCCCAAAAGAAATTGGGTTCCTTGATAACCTCAGTTTCCTTGATCTGTCTGAAAACCGTCTTGTTGGTATCGTACCAGAAGAAATTGGAAAATGCAGTTCATTACAGCTACTCAACTTAAGTAACAACACCCTTGGAGGTAATCTGCCTAGCTCCTTTTCTTCTCTCACAAGGCTTCAAGTACTGGATGTCTCTGTGAATCAATTTGTTGGTCAGATTCCTGAGAGTTACGGTGGACTTGCTGCACTTAATAGGCTCATCCTCCGTAGCAACTCCTTAACTGAGCCAATCCCCTCTTCACTTGGTAAATGTTCAAACCTTCAACTGCTTGATCTTAGTAGCAACAAGCTCACTGGGATGATCCCAGAGCAACTGTTTGAAATTGAAGCTCTTGACATTGCTTTAAACCTGAGTTTCAATGCTTTATATGGGATAATCCCACCACAGATTTCTGCTCTCAATAAGCTATCCATACTAGACCTTTCACACAATACGCTTGAAGGTGATCTGTTGGCACTTTCCGGGCTTATAAATCTTGTTTCTTTGAACATCTCATACAACAACTTCACTGGCTATCTTCCTGACAGCAAGGTGTTTCGACAGTTGTCAGCAACAGACTTGGTGGGAAACAAAGGATTGTGCTCTAGAGGTCATGATCTATGTTTCCTCAGCAATGGTACAACAATGGCCATCTCTAGAAGTGGCAGTTTGAGGAGGTCATGGAGACTTAAATTGGCCATTGCATTGCTAATAGCCTTGACCATTGCTTTGTCCATTTTTGGGGTAGTTGCAGTATTTCGAGCACGAAAGATGAATGGAGAGGACAATGATTCTGAGATTGGAGGGGACTCATGGCCTTGGCAATTCACTCCATTTCAGAAGGTGAGTTTCTCAGTTGAGCAAGTTCTAAAATGTCTAGTAGAAACCAATGTAATAGGAAAGGGATGCTCAGGAGTTGTTTACCGGGCAGAAATGGAAAACGAAGACATTGCAGTAAAGAAGCTCTGGCCAACAACAACTGCTTCCAAATATGATTGTCAAAGTAGAGGAGTTCGTGATTCCTTTTCGGCTGAGGTGAAGACCCTTGGCTCCATTCGCCACAAGAACATTGTTAGGTTCTTGGGTTGCTGTTGGAACCGAAACACAAGATTGCTTATGTATGAGTACATGCCAAATGGGAGCTTGGGTGGTTTACTTCATGAAAGAAGTGGTAACTGCTTAGAATGGGACATCAGATATCGCATAGTACTCGGGGCTGCGCAAGGCTTGGCTTATTTGCACCATGACTCTGTTCCTCCTATAGTTCACAGAGACATTAAGGCCAACAACATTCTCATTGGTCCAGATTTTGAGCCCTGCATTGCTGATTTTGGGCTTGCCAAGCTCGTTGATGAAGGAGATTTTGCTCGGTCCTCCAACACGGTTGCAGGTTCTTATGGCTACATTGCTCCTGAGTATGGATACTCAATGAAGATAACAGAGAAAAGCGATGTGTACAGCTACGGCGTAGTTGTCCTAGAAGTGCTAACAGGGAAGCAACCAATTGATCCAACCATACCAGATGGACTCCACATTGTGGACTGGGTAAGGCAGAAGAGAGGGGGAGTTGAGGTACTTGATCAAAGCTTACGAGCACGACCAGAACCCGAGATTGAGGAAATGCTGCAGACATTAGGTGTGGCATTGCTGTGCATTAACCCTACCCCGGATGACAGACCAACCATGAAAGATGTAGCAGCAATGCTCAAAGAGATTAGGCAGGAGAGAGAGGAGTGCATGAAAGCTAACATGAGGCTTCTCAATGGGTCCTATTCTGCAAATGATCATGGACAAGAAACTATGTTCAGTTGCAGTGGAGGAACATCATCAGCTATGGTGAAGCAACAACATACATACCCTCCAACAAGTACCAGCACAAGTTTCTCTGCTTCCTCATTGCTATACACTTCATCTTCCAATGTCAAAACAACTCTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1133

Amino Acids

123.29

Weight (kDa)

5.23

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 71 1e-05 Leucine rich repeat N-terminal domain
LRR_14 PF23598 213 - 299 1.9e-08 Leucine-rich repeat region
LRR_14 PF23598 306 - 394 1.2e-07 Leucine-rich repeat region
LRR_8 PF13855 315 - 375 4e-08 Leucine rich repeat
LRR_8 PF13855 460 - 519 6.2e-06 Leucine rich repeat
LRR_14 PF23598 464 - 681 3e-14 Leucine-rich repeat region
LRR_8 PF13855 484 - 543 2.5e-07 Leucine rich repeat
LRR_8 PF13855 557 - 616 7.9e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 783 - 1055 1.2e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 783 - 1053 2.1e-40 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48940
fragaria_vesca FvH4_2g19620
malus_domestica MD10G1169800.v1.1
prunus_persica Prupe.8G207600_v2.0.a1
pyrus_communis pycom10g14630
rosa_chinensis RchiOBHm_Chr6g0285351
rosa_laevigata RLG00000012651
rosa_multiflora Rmu_co8152086.1_g000001 Rmu_co8221716.1_g000001 Rmu_sc0012287.1_g000004
rosa_roxburghii Rroxscaffold_7G00183330
rosa_rugosa Rorug06G0173900
rosa_samantha Rh6AG284500 Rh6BG285700 Rh6CG286800 Rh6DG279800
rosa_wichuraiana Rw6G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1952
AarI CACCTGC 1 cut(s) 655
Acc16I TGCGCA 1 cut(s) 2682
Acc36I ACCTGC 2 cut(s) 655, 2840
AccB7I CCANNNNNTGG 1 cut(s) 2998
AclWI GGATC 4 cut(s) 1129, 1417, 1777, 2963
AcoI YGGCCR 4 cut(s) 1221, 2109, 2157, 2429
AcsI RAATTY 3 cut(s) 277, 973, 1306
AcuI CTGAAG 3 cut(s) 156, 428, 536
AdeI CACNNNGTG 1 cut(s) 2998
AfiI CCNNNNNNNGG 7 cut(s) 353, 788, 860, 2202, 2879, 2998, 3127
AflII CTTAAG 1 cut(s) 1540
AflIII ACRYGT 1 cut(s) 618
AjuI GAANNNNNNNTTGG 2 cut(s) 1702, 1734
Alw21I GWGCWC 4 cut(s) 1167, 2066, 2227, 3058
Alw26I GTCTC 4 cut(s) 970, 1618, 2140, 2727
AlwI GGATC 4 cut(s) 1129, 1417, 1777, 2963
AlwNI CAGNNNCTG 2 cut(s) 451, 742
Ama87I CYCGRG 2 cut(s) 2672, 3068
AoxI GGCC 8 cut(s) 8, 1221, 1288, 2109, 2157, 2276, 2429, 2742
ApoI RAATTY 3 cut(s) 277, 973, 1306
ArsI GACNNNNNNTTYG 2 cut(s) 2173, 2205
AseI ATTAAT 1 cut(s) 237
Asp700I GAANNNNTTC 2 cut(s) 441, 594
AspLEI GCGC 2 cut(s) 509, 2683
AspS9I GGNCC 6 cut(s) 195, 448, 1346, 2762, 2832, 3228
AsuC2I CCSGG 4 cut(s) 635, 1945, 2387, 3128
AsuHPI GGTGA 9 cut(s) 13, 129, 336, 512, 782, 1937, 2317, 2509, 3310
AsuNHI GCTAGC 1 cut(s) 256
AvaI CYCGRG 2 cut(s) 2672, 3068
AvaII GGWCC 6 cut(s) 195, 448, 1346, 2762, 2832, 3228
AxyI CCTNAGG 1 cut(s) 924
BaeI ACNNNNGTAYC 2 cut(s) 675, 708
BalI TGGCCA 3 cut(s) 2111, 2159, 2431
BanII GRGCYC 2 cut(s) 1167, 2778
BarI GAAGNNNNNNTAC 4 cut(s) 400, 432, 1267, 1299
BbsI GAAGAC 5 cut(s) 40, 959, 1229, 2412, 2507
Bbv12I GWGCWC 4 cut(s) 1167, 2066, 2227, 3058
BbvCI CCTCAGC 2 cut(s) 2090, 2493
BccI CCATC 6 cut(s) 32, 905, 1141, 1219, 2120, 2978
BceAI ACGGC 1 cut(s) 2941
BcgI CGANNNNNNTGC 4 cut(s) 2393, 2427, 2653, 2687
BciVI GTATCC 1 cut(s) 2876
BclI TGATCA 2 cut(s) 3040, 3244
BcnI CCSGG 4 cut(s) 635, 1945, 2387, 3128
BcoDI GTCTC 4 cut(s) 970, 1618, 2140, 2727
BfaI CTAG 9 cut(s) 6, 257, 1247, 1572, 1898, 2067, 2118, 2339, 2939
BfmI CTRYAG 5 cut(s) 734, 940, 1103, 2721, 3086
BfrI CTTAAG 1 cut(s) 1540
BfuAI ACCTGC 2 cut(s) 655, 2840
BfuI GTATCC 1 cut(s) 2876
BglII AGATCT 1 cut(s) 1249
BlpI GCTNAGC 1 cut(s) 725
BmcAI AGTACT 2 cut(s) 1605, 2670
Bme1390I CCNGG 4 cut(s) 635, 1945, 2387, 3128
Bme18I GGWCC 6 cut(s) 195, 448, 1346, 2762, 2832, 3228
BmeT110I CYCGRG 2 cut(s) 2672, 3068
BmgT120I GGNCC 6 cut(s) 195, 448, 1346, 2762, 2832, 3228
BmiI GGNNCC 6 cut(s) 167, 1347, 1442, 2512, 2558, 3229
BmrFI CCNGG 4 cut(s) 635, 1945, 2387, 3128
BmrI ACTGGG 3 cut(s) 578, 1785, 3013
BmsI GCATC 2 cut(s) 1023, 2357
BmtI GCTAGC 1 cut(s) 260
BmuI ACTGGG 3 cut(s) 578, 1785, 3013
BoxI GACNNNNGTC 1 cut(s) 2026
BpiI GAAGAC 5 cut(s) 40, 959, 1229, 2412, 2507
BpmI CTGGAG 2 cut(s) 624, 899
Bpu10I CCTNAGC 4 cut(s) 1391, 2090, 2371, 2493
Bpu1102I GCTNAGC 1 cut(s) 725
BpuEI CTTGAG 1 cut(s) 1043
BpuMI CCSGG 4 cut(s) 635, 1945, 2387, 3128
Bsa29I ATCGAT 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 1116
BsaJI CCNNGG 4 cut(s) 1555, 2279, 2506, 3126
BsaWI WCCGGW 1 cut(s) 781
BsaXI ACNNNNNCTCC 8 cut(s) 872, 902, 2593, 2623, 3018, 3048, 3304, 3334
Bsc4I CCNNNNNNNGG 7 cut(s) 353, 788, 860, 2202, 2879, 2998, 3127
Bse118I RCCGGY 1 cut(s) 1223
Bse21I CCTNAGG 1 cut(s) 924
Bse8I GATNNNNATC 1 cut(s) 1116
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 4 cut(s) 1555, 2279, 2506, 3126
BseGI GGATG 6 cut(s) 643, 1615, 1680, 1785, 2372, 3136
BseJI GATNNNNATC 1 cut(s) 1116
BseLI CCNNNNNNNGG 7 cut(s) 353, 788, 860, 2202, 2879, 2998, 3127
BseRI GAGGAG 4 cut(s) 1040, 2149, 2484, 3206
BseYI CCCAGC 1 cut(s) 1138
BsgI GTGCAG 2 cut(s) 1118, 1650
BshFI GGCC 8 cut(s) 10, 1223, 1290, 2111, 2159, 2278, 2431, 2744
BshVI ATCGAT 1 cut(s) 27
BsiHKAI GWGCWC 4 cut(s) 1167, 2066, 2227, 3058
BsiHKCI CYCGRG 2 cut(s) 2672, 3068
BsiSI CCGG 7 cut(s) 635, 782, 1224, 1387, 1944, 2386, 3128
BslFI GGGAC 3 cut(s) 928, 2282, 2663
BslI CCNNNNNNNGG 7 cut(s) 353, 788, 860, 2202, 2879, 2998, 3127
BsmAI GTCTC 4 cut(s) 970, 1618, 2140, 2727
BsmFI GGGAC 3 cut(s) 928, 2282, 2663
BsnI GGCC 8 cut(s) 10, 1223, 1290, 2111, 2159, 2278, 2431, 2744
BsoBI CYCGRG 2 cut(s) 2672, 3068
Bsp1286I GDGCHC 5 cut(s) 1167, 2066, 2227, 2778, 3058
Bsp1407I TGTACA 1 cut(s) 2916
Bsp1720I GCTNAGC 1 cut(s) 725
BspANI GGCC 8 cut(s) 10, 1223, 1290, 2111, 2159, 2278, 2431, 2744
BspDI ATCGAT 1 cut(s) 27
BspHI TCATGA 2 cut(s) 2074, 2620
BspLI GGNNCC 6 cut(s) 167, 1347, 1442, 2512, 2558, 3229
BspMAI CTGCAG 4 cut(s) 738, 944, 1107, 3090
BspMI ACCTGC 2 cut(s) 655, 2840
BspOI GCTAGC 1 cut(s) 260
BspPI GGATC 4 cut(s) 1129, 1417, 1777, 2963
BspTI CTTAAG 1 cut(s) 1540
BsrFI RCCGGY 1 cut(s) 1223
BsrGI TGTACA 1 cut(s) 2916
BssAI RCCGGY 1 cut(s) 1223
BssECI CCNNGG 4 cut(s) 1555, 2279, 2506, 3126
BssT1I CCWWGG 3 cut(s) 1555, 2279, 2506
Bst4CI ACNGT 9 cut(s) 398, 708, 797, 1171, 1481, 1655, 1797, 2025, 2845
Bst6I CTCTTC 3 cut(s) 148, 1723, 3011
BstAFI CTTAAG 1 cut(s) 1540
BstAPI GCANNNNNTGC 1 cut(s) 65
BstAUI TGTACA 1 cut(s) 2916
BstC8I GCNNGC 7 cut(s) 258, 431, 511, 678, 731, 866, 2799
BstF5I GGATG 6 cut(s) 643, 1615, 1680, 1785, 2372, 3136
BstHHI GCGC 2 cut(s) 509, 2683
BstMAI GTCTC 4 cut(s) 970, 1618, 2140, 2727
BstMWI GCNNNNNNNGC 9 cut(s) 65, 77, 376, 730, 739, 874, 1105, 2517, 2803
BstNSI RCATGY 3 cut(s) 622, 733, 2593
BstPAI GACNNNNGTC 1 cut(s) 2026
BstSCI CCNGG 4 cut(s) 633, 1943, 2385, 3126
BstSFI CTRYAG 5 cut(s) 734, 940, 1103, 2721, 3086
BstV2I GAAGAC 5 cut(s) 40, 959, 1229, 2412, 2507
BstX2I RGATCY 2 cut(s) 1249, 1422
BstXI CCANNNNNNTGG 2 cut(s) 476, 911
BstYI RGATCY 2 cut(s) 1249, 1422
Bsu15I ATCGAT 1 cut(s) 27
Bsu36I CCTNAGG 1 cut(s) 924
BsuI GTATCC 1 cut(s) 2876
BsuRI GGCC 8 cut(s) 10, 1223, 1290, 2111, 2159, 2278, 2431, 2744
BsuTUI ATCGAT 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 2925
BtsCI GGATG 6 cut(s) 643, 1615, 1680, 1785, 2372, 3136
BtsI GCAGTG 1 cut(s) 3280
BtsIMutI CAGTG 7 cut(s) 228, 566, 1269, 1420, 1773, 1989, 3280
BveI ACCTGC 2 cut(s) 655, 2840
Cac8I GCNNGC 7 cut(s) 258, 431, 511, 678, 731, 866, 2799
CaiI CAGNNNCTG 2 cut(s) 451, 742
CciI TCATGA 2 cut(s) 2074, 2620
CfoI GCGC 2 cut(s) 509, 2683
Cfr10I RCCGGY 1 cut(s) 1223
Cfr13I GGNCC 6 cut(s) 195, 448, 1346, 2762, 2832, 3228
ClaI ATCGAT 1 cut(s) 27
CseI GACGC 1 cut(s) 748
CspCI CAANNNNNGTGG 2 cut(s) 840, 875
DraI TTTAAA 1 cut(s) 1827
DraIII CACNNNGTG 1 cut(s) 2998
EaeI YGGCCR 4 cut(s) 1221, 2109, 2157, 2429
Eam1104I CTCTTC 3 cut(s) 148, 1723, 3011
EarI CTCTTC 3 cut(s) 148, 1723, 3011
Ecl136II GAGCTC 1 cut(s) 1165
Eco130I CCWWGG 3 cut(s) 1555, 2279, 2506
Eco24I GRGCYC 2 cut(s) 1167, 2778
Eco32I GATATC 1 cut(s) 2660
Eco47I GGWCC 6 cut(s) 195, 448, 1346, 2762, 2832, 3228
Eco53kI GAGCTC 1 cut(s) 1165
Eco57I CTGAAG 3 cut(s) 156, 428, 536
Eco81I CCTNAGG 1 cut(s) 924
Eco88I CYCGRG 2 cut(s) 2672, 3068
EcoICRI GAGCTC 1 cut(s) 1165
EcoO109I RGGNCCY 2 cut(s) 448, 3228
EcoRV GATATC 1 cut(s) 2660
EcoT14I CCWWGG 3 cut(s) 1555, 2279, 2506
EcoT38I GRGCYC 2 cut(s) 1167, 2778
ErhI CCWWGG 3 cut(s) 1555, 2279, 2506
FalI AAGNNNNNCTT 2 cut(s) 1307, 1339
FaqI GGGAC 3 cut(s) 928, 2282, 2663
FbaI TGATCA 2 cut(s) 3040, 3244
FokI GGATG 6 cut(s) 650, 1622, 1667, 1792, 2379, 3143
FriOI GRGCYC 2 cut(s) 1167, 2778
FspBI CTAG 9 cut(s) 6, 257, 1247, 1572, 1898, 2067, 2118, 2339, 2939
FspI TGCGCA 1 cut(s) 2682
GlaI GCGC 2 cut(s) 508, 2682
GsaI CCCAGC 1 cut(s) 1142
GsuI CTGGAG 2 cut(s) 624, 899
HaeIII GGCC 8 cut(s) 10, 1223, 1290, 2111, 2159, 2278, 2431, 2744
HapII CCGG 7 cut(s) 635, 782, 1224, 1387, 1944, 2386, 3128
HgaI GACGC 1 cut(s) 748
HhaI GCGC 2 cut(s) 509, 2683
Hin6I GCGC 2 cut(s) 507, 2681
HinP1I GCGC 2 cut(s) 507, 2681
HincII GTYRAC 2 cut(s) 94, 811
HindII GTYRAC 2 cut(s) 94, 811
HindIII AAGCTT 4 cut(s) 866, 946, 1316, 3046
HpaII CCGG 7 cut(s) 635, 782, 1224, 1387, 1944, 2386, 3128
HphI GGTGA 9 cut(s) 13, 129, 336, 512, 782, 1937, 2317, 2509, 3310
HpyCH4III ACNGT 9 cut(s) 398, 708, 797, 1171, 1481, 1655, 1797, 2025, 2845
HpyCH4IV ACGT 3 cut(s) 62, 96, 384
HpyF10VI GCNNNNNNNGC 9 cut(s) 65, 77, 376, 730, 739, 874, 1105, 2517, 2803
HpySE526I ACGT 3 cut(s) 62, 96, 384
HspAI GCGC 2 cut(s) 507, 2681
Ksp22I TGATCA 2 cut(s) 3040, 3244
LmnI GCTCC 7 cut(s) 493, 1027, 1162, 1580, 2516, 2601, 2875
LweI GCATC 2 cut(s) 1023, 2357
MaeI CTAG 9 cut(s) 6, 257, 1247, 1572, 1898, 2067, 2118, 2339, 2939
MaeII ACGT 3 cut(s) 62, 96, 384
MaeIII GTNAC 7 cut(s) 226, 576, 1040, 1274, 1544, 1649, 2633
MfeI CAATTG 1 cut(s) 2964
MflI RGATCY 2 cut(s) 1249, 1422
MhlI GDGCHC 5 cut(s) 1167, 2066, 2227, 2778, 3058
MlsI TGGCCA 3 cut(s) 2111, 2159, 2431
MluNI TGGCCA 3 cut(s) 2111, 2159, 2431
MlyI GAGTC 4 cut(s) 1243, 2264, 2702, 2982
MmeI TCCRAC 5 cut(s) 183, 2534, 2862, 2996, 3347
Mox20I TGGCCA 3 cut(s) 2111, 2159, 2431
MroXI GAANNNNTTC 2 cut(s) 441, 594
MscI TGGCCA 3 cut(s) 2111, 2159, 2431
MslI CAYNNNNRTG 3 cut(s) 909, 1778, 3109
Msp20I TGGCCA 3 cut(s) 2111, 2159, 2431
MspA1I CMGCKG 3 cut(s) 1102, 1142, 1300
MspCI CTTAAG 1 cut(s) 1540
MspI CCGG 7 cut(s) 635, 782, 1224, 1387, 1944, 2386, 3128
MspR9I CCNGG 4 cut(s) 635, 1945, 2387, 3128
MunI CAATTG 1 cut(s) 2964
MwoI GCNNNNNNNGC 9 cut(s) 65, 77, 376, 730, 739, 874, 1105, 2517, 2803
NciI CCSGG 4 cut(s) 635, 1945, 2387, 3128
NheI GCTAGC 1 cut(s) 256
NlaIV GGNNCC 6 cut(s) 167, 1347, 1442, 2512, 2558, 3229
NmuCI GTSAC 1 cut(s) 226
NsbI TGCGCA 1 cut(s) 2682
NspI RCATGY 3 cut(s) 622, 733, 2593
PaeI GCATGC 1 cut(s) 733
PagI TCATGA 2 cut(s) 2074, 2620
PaqCI CACCTGC 1 cut(s) 655
PciI ACATGT 1 cut(s) 618
PdmI GAANNNNTTC 2 cut(s) 441, 594
PfeI GAWTC 7 cut(s) 520, 775, 1410, 1621, 1639, 2252, 2480
PflFI GACNNNGTC 1 cut(s) 742
PflMI CCANNNNNTGG 1 cut(s) 2998
PfoI TCCNGGA 1 cut(s) 633
PleI GAGTC 4 cut(s) 1242, 2264, 2702, 2982
PpsI GAGTC 4 cut(s) 1242, 2264, 2702, 2982
PpuMI RGGWCCY 2 cut(s) 448, 3228
PscI ACATGT 1 cut(s) 618
PshAI GACNNNNGTC 1 cut(s) 2026
PshBI ATTAAT 1 cut(s) 237
PsiI TTATAA 1 cut(s) 1952
Psp124BI GAGCTC 1 cut(s) 1167
Psp5II RGGWCCY 2 cut(s) 448, 3228
PspFI CCCAGC 1 cut(s) 1138
PspN4I GGNNCC 6 cut(s) 167, 1347, 1442, 2512, 2558, 3229
PspPI GGNCC 6 cut(s) 195, 448, 1346, 2762, 2832, 3228
PspPPI RGGWCCY 2 cut(s) 448, 3228
PsrI GAACNNNNNNTAC 2 cut(s) 1961, 1993
PstI CTGCAG 4 cut(s) 738, 944, 1107, 3090
PstNI CAGNNNCTG 2 cut(s) 451, 742
PsuI RGATCY 2 cut(s) 1249, 1422
PsyI GACNNNGTC 1 cut(s) 742
PvuII CAGCTG 3 cut(s) 1102, 1142, 1300
RseI CAYNNNNRTG 3 cut(s) 909, 1778, 3109
SacI GAGCTC 1 cut(s) 1167
Sau96I GGNCC 6 cut(s) 195, 448, 1346, 2762, 2832, 3228
ScaI AGTACT 2 cut(s) 1605, 2670
SchI GAGTC 4 cut(s) 1243, 2264, 2702, 2982
ScrFI CCNGG 4 cut(s) 635, 1945, 2387, 3128
SduI GDGCHC 5 cut(s) 1167, 2066, 2227, 2778, 3058
SfaNI GCATC 2 cut(s) 1023, 2357
SfcI CTRYAG 5 cut(s) 734, 940, 1103, 2721, 3086
SinI GGWCC 6 cut(s) 195, 448, 1346, 2762, 2832, 3228
SmiMI CAYNNNNRTG 3 cut(s) 909, 1778, 3109
SmlI CTYRAG 2 cut(s) 1022, 1540
SmoI CTYRAG 2 cut(s) 1022, 1540
SphI GCATGC 1 cut(s) 733
SspMI CTAG 9 cut(s) 6, 257, 1247, 1572, 1898, 2067, 2118, 2339, 2939
SstI GAGCTC 1 cut(s) 1167
StyD4I CCNGG 4 cut(s) 633, 1943, 2385, 3126
StyI CCWWGG 3 cut(s) 1555, 2279, 2506
TaaI ACNGT 9 cut(s) 398, 708, 797, 1171, 1481, 1655, 1797, 2025, 2845
TaiI ACGT 3 cut(s) 65, 99, 387
TaqI TCGA 6 cut(s) 27, 598, 746, 1197, 2020, 2221
TaqII GACCGA 1 cut(s) 2820
TatI WGTACW 5 cut(s) 1373, 1603, 2586, 2668, 2916
TfiI GAWTC 7 cut(s) 520, 775, 1410, 1621, 1639, 2252, 2480
TscAI CASTG 7 cut(s) 235, 573, 1276, 1420, 1780, 1996, 3280
TseFI GTSAC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 226
TspGWI ACGGA 1 cut(s) 1676
TspRI CASTG 7 cut(s) 235, 573, 1276, 1420, 1780, 1996, 3280
Tth111I GACNNNGTC 1 cut(s) 742
Van91I CCANNNNNTGG 1 cut(s) 2998
Vha464I CTTAAG 1 cut(s) 1540
VpaK11BI GGWCC 6 cut(s) 195, 448, 1346, 2762, 2832, 3228
VspI ATTAAT 1 cut(s) 237
XapI RAATTY 3 cut(s) 277, 973, 1306
XbaI TCTAGA 3 cut(s) 1246, 2066, 2117
XceI RCATGY 3 cut(s) 622, 733, 2593
XmnI GAANNNNTTC 2 cut(s) 441, 594
XspI CTAG 9 cut(s) 6, 257, 1247, 1572, 1898, 2067, 2118, 2339, 2939
ZrmI AGTACT 2 cut(s) 1605, 2670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.