Rh6AG284500
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013816
Physical Location & Seq
Forward (+)
48031147 .. 48035030
3884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG284500.1

Sequence Viewer

Length: 3453 bp
ATGCCAATGCTGTTGAGGCAGCAGCCCTCTAACACCTACAATCACCACCAAATGTCTAGGCCACTATCGTTCACCATCGATTTCTTCTTCCTCTTTGTCTTCACACTCGCAACGTCTGCTGCTTCTTTGGCTGCTGCAAATGTTGACGTTCTGACATTACATACATGGCTTCAAGGCTCTACTACCTCACCCTCTTCTTCTGATTTCTCCGACTGGAACCCTTCAGACCCCAACCCTTGTAATTGGTCCTACATTACTTGTTCTTCTCAAAACTTTGTCACTGAAATTAATATCCAGTTTATTGAGCTAGCCCTTCCCTTTCCTTCAAATTTTTCCTCCCTTGCATTTCTTCAAAAGCTCGTCATTTCTGGTGCTAATCTTACTGGAACAATCTCACCTGATATTGGATATTGCAAATCGCTTCTGGTGCTTGACGTAAGTTCAAACAGTCTTGTGGGTAGTATTCCTTCAAGTATTGGCAGGCTTGAAAAGCTTCAGGACCTGATCTTAAACTCTAACCAGCTTACTGGTCAAGTACCAATGGAGCTTGGTGATTGCGCCAGCCTGAAGAATCTTCTCATATTTGATAACTACCTAAGTGGTTATCTTCCTGCTACACTCGGAAAGTTACTAAACTTGGAAGTCATTCGAGCTGGAGGGAACAAAGACGTGTCAGGAAATATCCCGGATGAGCTTGGAAACTGCAAGAACTTGCAGGTGTTGGGGCTTGCTGATACTAAAGTTTCTGGTTTGATACCTTCATCATTGGGTAAGCTCAGCATGCTGCAGACGCTGTCGATTTATACTACTATGCTATCGGGTGAGATTCCACCGGATATTGGCAACTGTTCAGAACTTGTCAACTTGTTCTTGTATGAGAATGACCTTTCTGGTTCACTACCACCAGAGTTGGGCAAGCTTCATAAGCTGGAGAAGATGTTATTGTGGCAAAACAACCTTGATGGGACTATTCCTGAGGAAATTGGGAACTGCAGAAGCTTGAAGACCATAGATGTCTCTCTAAATTCTTTTTCTGGAAGCATACCTCAGTCTTTCGGAAACCTCTCAAATCTTGAGGAGCTTATGCTTAGTAACAACAACATCTCTGGTTCAATTCCCTCAGTTCTTTCCAATGCTACAAAACTTGTGCAGCTGCAGCTTGATACTAATCAGATTTCAGGCTTGATCCCAGCTGAGATGGGAATGTTGAAGGAGCTCACAGTCTTTTTTGCTTGGCAGAACAAACTCGAAGGTAGTATTCCATCAGAATTGGCCGGTTGTGAGAGTCTTCAAGCTCTAGATCTCTCACACAATGCTCTCACTGGTAACATACCTTCTGGCCTATTTCAGCTGAAAAATTTAACCAAGCTTCTCTTGATTTCTAATGACATTTCGGGTCCAATACCTTCGGTGCTTGGAAATTGTACTTCTCTTATCCGGCTAAGGCTTGTGCATAACAGAATCAGTGGGGAGATCCCAAAAGAAATTGGGTTCCTTGATAACCTCAGTTTCCTTGATCTGTCTGAAAACCGTCTTGTTGGTATCGTACCAGAAGAAATTGGAAAATGCAGTTCATTACAGCTACTCAACTTAAGTAACAACACCCTTGGAGGTAATCTGCCTAGCTCCTTTTCTTCTCTCACAAGGCTTCAAGTACTGGATGTCTCTGTGAATCAATTTGTAGGTCAGATTCCTGAGAGTTACGGTGGACTTGCTGCACTTAATAGGCTCATCCTCCGTAGCAACTCCTTAACTGAGCCAATCCCCTCTTCACTTGGTAAATGTTCAAACCTTCAACTGCTTGATCTTAGTAGCAACAAGCTCACTGGGATGATCCCAGAGCAACTGTTTGAAATTGAAGCTCTTGACATTGCTTTGAACCTGAGTTTCAATGCTTTATATGGGATAATCCCACCACAGATTTCTGCTCTCAATAAGCTATCCATACTAGACCTTTCACACAATAAGCTTGAAGGTGATCTGTTGGCACTTTCCGGGCTTATAAATCTTGTTTCTTTGAACATCTCATACAACAACTTCACTGGCTATCTTCCTGACAGCAAGGTGTTTCGACAGTTGTCAGCAACAGACTTGTTGGGAAACAAAGGATTGTGCTCTAGAGGTCATGATCTATGTTTCCTCAGCAATGGTACAACAATGGCCATGTCTAGAAGTGGCAGTTTGGGGAGGTCATGGCGACTTAAATTGGCCATTGCATTGCTAATAGCCTTGACCATTGCTTTGTCCATTTTTGGGGTAGTTGCAGTATTTCGAGCACGAAAGATGAATGGAGAGGACAATGATTCTGAGATTGGAGGGGACTCATGGCCTTGGCAATTCACTCCATTTCAGAAGGTGAGTTTCTCAGTTGAGCAAGTTCTAAAATGTCTAGTAGAAACCAATGTAATAGGAAAGGGATGCTCAGGAGTTGTTTACCGGGCAGAAATGGAAAACGAAGACATTGCAGTAAAGAAGCTCTGGCCAACAACAACTGCTTCCAAATATGATTGTCAAAGTAGAGGAGTTCGTGATTCCTTTTCGGCTGAGGTGAAGACCCTTGGCTCCATTCGCCACAAGAACATTGTTAGGTTCTTGGGTTGCTGTTGGAACCGAAACACAAGATTGCTTATGTATGAGTACATGCCAAATGGGAGCTTGGGTGGTTTACTTCATGAAAGAAGTGGTAACTGCTTAGAATGGGACATCAGATATCGCATAGTACTCGGGGCTGCGCAAGGCTTGGCTTATTTGCACCATGACTCTGTTCCTCCTATAGTTCACAGAGACATTAAGGCCAACAACATTCTCATTGGTCCAGATTTTGAGCCCTGCATTGCTGATTTTGGGCTTGCCAAGCTTGTTGATGAAGGAGATTTTGCTCGGTCCTCCAACACGGTTGCAGGTTCTTATGGCTACATTGCTCCTGAGTATGGATACTCAATGAAGATAACAGAGAAAAGCGATGTGTACAGCTACGGCGTAGTTGTGCTAGAAGTGCTAACAGGGAAGCAACCAATTGATCCAACCATACCAGATGGACTCCACATTGTGGATTGGGTAAGGCAGAAGAGAGGGGGAGTTGAGGTACTTGATCAAAGCTTACGGGCACGACCAGAACCAGAGATTGAGGAAATGCTGCAGACATTAGGTGTGGCATTGCTGTGCATTAACCCTACCCCGGATGACAGACCAACCATGAAAGATGTAGCAGCAATGCTCAAAGAGATTAGGCAGGAGAGAGAGGAGTGCATGAAAGCTAACATGAGGCTTCTCAATGGGTCCAATTCTGCAAATGATCATGGACAAGAAACTATGGTCAGTTGCAATGGAGGAACATCATCAGCTATGGTGAAGCAACAACATCCATACCCTCCAACAAGTACCAGCACAAGTTTCTCTGCTTCCTCATTGCTATACACTTCATCTTCCAATGTCAAAACAACTCTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1150

Amino Acids

125.24

Weight (kDa)

5.34

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 114 - 217 8.4e-06 Leucine-rich repeat region
LRR_14 PF23598 223 - 317 1.1e-08 Leucine-rich repeat region
LRR_14 PF23598 304 - 409 4.8e-08 Leucine-rich repeat region
LRR_8 PF13855 332 - 392 4.7e-08 Leucine rich repeat
LRR_14 PF23598 481 - 697 7e-14 Leucine-rich repeat region
LRR_8 PF13855 501 - 560 2.5e-07 Leucine rich repeat
LRR_8 PF13855 574 - 633 6.7e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 800 - 1072 1.2e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 800 - 1070 2.2e-40 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48940
fragaria_vesca FvH4_2g19620
malus_domestica MD10G1169800.v1.1
prunus_persica Prupe.8G207600_v2.0.a1
pyrus_communis pycom10g14630
rosa_chinensis RchiOBHm_Chr6g0285351
rosa_laevigata RLG00000012651
rosa_multiflora Rmu_co8152086.1_g000001 Rmu_co8221716.1_g000001 Rmu_sc0012287.1_g000004
rosa_roxburghii Rroxscaffold_7G00183330
rosa_rugosa Rorug06G0173900
rosa_samantha Rh6AG284500 Rh6BG285700 Rh6CG286800 Rh6DG279800
rosa_wichuraiana Rw6G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2003
AarI CACCTGC 1 cut(s) 706
Acc16I TGCGCA 1 cut(s) 2733
Acc36I ACCTGC 2 cut(s) 706, 2891
AccB7I CCANNNNNTGG 1 cut(s) 3049
AclWI GGATC 4 cut(s) 1180, 1468, 1828, 3014
AcoI YGGCCR 4 cut(s) 1272, 2160, 2208, 2480
AcsI RAATTY 3 cut(s) 328, 1024, 1357
AcuI CTGAAG 3 cut(s) 207, 479, 587
AdeI CACNNNGTG 1 cut(s) 3049
AfiI CCNNNNNNNGG 7 cut(s) 404, 839, 911, 2253, 2930, 3049, 3178
AflII CTTAAG 1 cut(s) 1591
AflIII ACRYGT 1 cut(s) 669
AjiI CACGTC 1 cut(s) 670
AjuI GAANNNNNNNTTGG 2 cut(s) 1753, 1785
Alw21I GWGCWC 3 cut(s) 1218, 2117, 2278
Alw26I GTCTC 3 cut(s) 1021, 1669, 2778
AlwI GGATC 4 cut(s) 1180, 1468, 1828, 3014
AlwNI CAGNNNCTG 2 cut(s) 502, 793
Ama87I CYCGRG 1 cut(s) 2723
AoxI GGCC 8 cut(s) 59, 1272, 1339, 2160, 2208, 2327, 2480, 2793
ApoI RAATTY 3 cut(s) 328, 1024, 1357
ArsI GACNNNNNNTTYG 2 cut(s) 2224, 2256
AseI ATTAAT 1 cut(s) 288
Asp700I GAANNNNTTC 2 cut(s) 492, 645
AspLEI GCGC 2 cut(s) 560, 2734
AspS9I GGNCC 6 cut(s) 246, 499, 1397, 2813, 2883, 3279
AsuC2I CCSGG 4 cut(s) 686, 1996, 2438, 3179
AsuNHI GCTAGC 1 cut(s) 307
AvaI CYCGRG 1 cut(s) 2723
AvaII GGWCC 6 cut(s) 246, 499, 1397, 2813, 2883, 3279
AxyI CCTNAGG 1 cut(s) 975
BaeGI GKGCMC 1 cut(s) 3109
BaeI ACNNNNGTAYC 2 cut(s) 726, 759
BalI TGGCCA 3 cut(s) 2162, 2210, 2482
BanII GRGCYC 2 cut(s) 1218, 2829
BarI GAAGNNNNNNTAC 4 cut(s) 451, 483, 1318, 1350
BbsI GAAGAC 5 cut(s) 91, 1010, 1280, 2463, 2558
Bbv12I GWGCWC 3 cut(s) 1218, 2117, 2278
BbvCI CCTCAGC 2 cut(s) 2141, 2544
BccI CCATC 5 cut(s) 83, 956, 1192, 1270, 3029
BceAI ACGGC 1 cut(s) 2992
BcgI CGANNNNNNTGC 4 cut(s) 2444, 2478, 2704, 2738
BciVI GTATCC 1 cut(s) 2927
BclI TGATCA 2 cut(s) 3091, 3295
BcnI CCSGG 4 cut(s) 686, 1996, 2438, 3179
BcoDI GTCTC 3 cut(s) 1021, 1669, 2778
BfaI CTAG 9 cut(s) 57, 308, 1298, 1623, 1949, 2118, 2169, 2390, 2990
BfmI CTRYAG 5 cut(s) 785, 991, 1154, 2772, 3137
BfrI CTTAAG 1 cut(s) 1591
BfuAI ACCTGC 2 cut(s) 706, 2891
BfuI GTATCC 1 cut(s) 2927
BglII AGATCT 1 cut(s) 1300
BlpI GCTNAGC 1 cut(s) 776
BmcAI AGTACT 2 cut(s) 1656, 2721
Bme1390I CCNGG 4 cut(s) 686, 1996, 2438, 3179
Bme18I GGWCC 6 cut(s) 246, 499, 1397, 2813, 2883, 3279
BmeT110I CYCGRG 1 cut(s) 2723
BmgBI CACGTC 1 cut(s) 670
BmgT120I GGNCC 6 cut(s) 246, 499, 1397, 2813, 2883, 3279
BmiI GGNNCC 6 cut(s) 218, 1398, 1493, 2563, 2609, 3280
BmrFI CCNGG 4 cut(s) 686, 1996, 2438, 3179
BmrI ACTGGG 1 cut(s) 1836
BmsI GCATC 1 cut(s) 2408
BmtI GCTAGC 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 1836
BoxI GACNNNNGTC 1 cut(s) 2077
BpiI GAAGAC 5 cut(s) 91, 1010, 1280, 2463, 2558
BpmI CTGGAG 2 cut(s) 675, 950
Bpu10I CCTNAGC 4 cut(s) 1442, 2141, 2422, 2544
Bpu1102I GCTNAGC 1 cut(s) 776
BpuEI CTTGAG 1 cut(s) 1094
BpuMI CCSGG 4 cut(s) 686, 1996, 2438, 3179
Bsa29I ATCGAT 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 1167
BsaJI CCNNGG 4 cut(s) 1606, 2330, 2557, 3177
BsaWI WCCGGW 1 cut(s) 832
BsaXI ACNNNNNCTCC 6 cut(s) 923, 953, 2644, 2674, 3069, 3099
Bsc4I CCNNNNNNNGG 7 cut(s) 404, 839, 911, 2253, 2930, 3049, 3178
Bse118I RCCGGY 1 cut(s) 1274
Bse1I ACTGG 8 cut(s) 218, 295, 388, 532, 1327, 1662, 1831, 2047
Bse21I CCTNAGG 1 cut(s) 975
Bse8I GATNNNNATC 1 cut(s) 1167
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 4 cut(s) 1606, 2330, 2557, 3177
BseGI GGATG 7 cut(s) 694, 1666, 1731, 1836, 2423, 3187, 3361
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 7 cut(s) 404, 839, 911, 2253, 2930, 3049, 3178
BseNI ACTGG 8 cut(s) 218, 295, 388, 532, 1327, 1662, 1831, 2047
BseRI GAGGAG 3 cut(s) 1091, 2535, 3257
BseSI GKGCMC 1 cut(s) 3109
BseYI CCCAGC 1 cut(s) 1189
BsgI GTGCAG 2 cut(s) 1169, 1701
BshFI GGCC 8 cut(s) 61, 1274, 1341, 2162, 2210, 2329, 2482, 2795
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 3 cut(s) 1218, 2117, 2278
BsiHKCI CYCGRG 1 cut(s) 2723
BsiSI CCGG 7 cut(s) 686, 833, 1275, 1438, 1995, 2437, 3179
BslFI GGGAC 3 cut(s) 979, 2333, 2714
BslI CCNNNNNNNGG 7 cut(s) 404, 839, 911, 2253, 2930, 3049, 3178
BsmAI GTCTC 3 cut(s) 1021, 1669, 2778
BsmFI GGGAC 3 cut(s) 979, 2333, 2714
BsnI GGCC 8 cut(s) 61, 1274, 1341, 2162, 2210, 2329, 2482, 2795
BsoBI CYCGRG 1 cut(s) 2723
Bsp1286I GDGCHC 5 cut(s) 1218, 2117, 2278, 2829, 3109
Bsp1407I TGTACA 1 cut(s) 2967
Bsp1720I GCTNAGC 1 cut(s) 776
BspANI GGCC 8 cut(s) 61, 1274, 1341, 2162, 2210, 2329, 2482, 2795
BspDI ATCGAT 1 cut(s) 78
BspHI TCATGA 2 cut(s) 2125, 2671
BspLI GGNNCC 6 cut(s) 218, 1398, 1493, 2563, 2609, 3280
BspMAI CTGCAG 4 cut(s) 789, 995, 1158, 3141
BspMI ACCTGC 2 cut(s) 706, 2891
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 4 cut(s) 1180, 1468, 1828, 3014
BspTI CTTAAG 1 cut(s) 1591
BsrFI RCCGGY 1 cut(s) 1274
BsrGI TGTACA 1 cut(s) 2967
BsrI ACTGG 8 cut(s) 218, 295, 388, 532, 1327, 1662, 1831, 2047
BssAI RCCGGY 1 cut(s) 1274
BssECI CCNNGG 4 cut(s) 1606, 2330, 2557, 3177
BssT1I CCWWGG 3 cut(s) 1606, 2330, 2557
Bst4CI ACNGT 8 cut(s) 449, 848, 1222, 1532, 1706, 1848, 2076, 2896
Bst6I CTCTTC 3 cut(s) 199, 1774, 3062
BstAFI CTTAAG 1 cut(s) 1591
BstAPI GCANNNNNTGC 1 cut(s) 116
BstAUI TGTACA 1 cut(s) 2967
BstC8I GCNNGC 7 cut(s) 309, 482, 562, 729, 782, 917, 2850
BstF5I GGATG 7 cut(s) 694, 1666, 1731, 1836, 2423, 3187, 3361
BstHHI GCGC 2 cut(s) 560, 2734
BstMAI GTCTC 3 cut(s) 1021, 1669, 2778
BstNSI RCATGY 2 cut(s) 784, 2644
BstPAI GACNNNNGTC 1 cut(s) 2077
BstSCI CCNGG 4 cut(s) 684, 1994, 2436, 3177
BstSFI CTRYAG 5 cut(s) 785, 991, 1154, 2772, 3137
BstSLI GKGCMC 1 cut(s) 3109
BstV2I GAAGAC 5 cut(s) 91, 1010, 1280, 2463, 2558
BstX2I RGATCY 2 cut(s) 1300, 1473
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 2 cut(s) 1300, 1473
Bsu15I ATCGAT 1 cut(s) 78
Bsu36I CCTNAGG 1 cut(s) 975
BsuI GTATCC 1 cut(s) 2927
BsuRI GGCC 8 cut(s) 61, 1274, 1341, 2162, 2210, 2329, 2482, 2795
BsuTUI ATCGAT 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 2976
BtrI CACGTC 1 cut(s) 670
BtsCI GGATG 7 cut(s) 694, 1666, 1731, 1836, 2423, 3187, 3361
BtsIMutI CAGTG 5 cut(s) 279, 1320, 1471, 1824, 2040
BveI ACCTGC 2 cut(s) 706, 2891
Cac8I GCNNGC 7 cut(s) 309, 482, 562, 729, 782, 917, 2850
CaiI CAGNNNCTG 2 cut(s) 502, 793
CciI TCATGA 2 cut(s) 2125, 2671
CfoI GCGC 2 cut(s) 560, 2734
Cfr10I RCCGGY 1 cut(s) 1274
Cfr13I GGNCC 6 cut(s) 246, 499, 1397, 2813, 2883, 3279
ClaI ATCGAT 1 cut(s) 78
CseI GACGC 1 cut(s) 799
CspCI CAANNNNNGTGG 2 cut(s) 891, 926
DraIII CACNNNGTG 1 cut(s) 3049
EaeI YGGCCR 4 cut(s) 1272, 2160, 2208, 2480
Eam1104I CTCTTC 3 cut(s) 199, 1774, 3062
EarI CTCTTC 3 cut(s) 199, 1774, 3062
Ecl136II GAGCTC 1 cut(s) 1216
Eco130I CCWWGG 3 cut(s) 1606, 2330, 2557
Eco24I GRGCYC 2 cut(s) 1218, 2829
Eco32I GATATC 1 cut(s) 2711
Eco47I GGWCC 6 cut(s) 246, 499, 1397, 2813, 2883, 3279
Eco53kI GAGCTC 1 cut(s) 1216
Eco57I CTGAAG 3 cut(s) 207, 479, 587
Eco81I CCTNAGG 1 cut(s) 975
Eco88I CYCGRG 1 cut(s) 2723
EcoICRI GAGCTC 1 cut(s) 1216
EcoO109I RGGNCCY 1 cut(s) 499
EcoRV GATATC 1 cut(s) 2711
EcoT14I CCWWGG 3 cut(s) 1606, 2330, 2557
EcoT38I GRGCYC 2 cut(s) 1218, 2829
ErhI CCWWGG 3 cut(s) 1606, 2330, 2557
FalI AAGNNNNNCTT 2 cut(s) 1358, 1390
FaqI GGGAC 3 cut(s) 979, 2333, 2714
FbaI TGATCA 2 cut(s) 3091, 3295
FokI GGATG 7 cut(s) 701, 1673, 1718, 1843, 2430, 3194, 3348
FriOI GRGCYC 2 cut(s) 1218, 2829
FspBI CTAG 9 cut(s) 57, 308, 1298, 1623, 1949, 2118, 2169, 2390, 2990
FspI TGCGCA 1 cut(s) 2733
GlaI GCGC 2 cut(s) 559, 2733
GsaI CCCAGC 1 cut(s) 1193
GsuI CTGGAG 2 cut(s) 675, 950
HaeIII GGCC 8 cut(s) 61, 1274, 1341, 2162, 2210, 2329, 2482, 2795
HapII CCGG 7 cut(s) 686, 833, 1275, 1438, 1995, 2437, 3179
HgaI GACGC 1 cut(s) 799
HhaI GCGC 2 cut(s) 560, 2734
Hin6I GCGC 2 cut(s) 558, 2732
HinP1I GCGC 2 cut(s) 558, 2732
HincII GTYRAC 2 cut(s) 145, 862
HindII GTYRAC 2 cut(s) 145, 862
HindIII AAGCTT 7 cut(s) 491, 917, 997, 1367, 1967, 2855, 3097
HpaII CCGG 7 cut(s) 686, 833, 1275, 1438, 1995, 2437, 3179
Hpy166II GTNNAC 9 cut(s) 72, 145, 862, 896, 1709, 2434, 2666, 2779, 2968
Hpy8I GTNNAC 9 cut(s) 72, 145, 862, 896, 1709, 2434, 2666, 2779, 2968
HpyCH4III ACNGT 8 cut(s) 449, 848, 1222, 1532, 1706, 1848, 2076, 2896
HpyCH4IV ACGT 4 cut(s) 113, 147, 435, 669
HpySE526I ACGT 4 cut(s) 113, 147, 435, 669
HspAI GCGC 2 cut(s) 558, 2732
Ksp22I TGATCA 2 cut(s) 3091, 3295
LmnI GCTCC 7 cut(s) 544, 1078, 1213, 1631, 2567, 2652, 2926
LweI GCATC 1 cut(s) 2408
MaeI CTAG 9 cut(s) 57, 308, 1298, 1623, 1949, 2118, 2169, 2390, 2990
MaeII ACGT 4 cut(s) 113, 147, 435, 669
MaeIII GTNAC 7 cut(s) 277, 627, 1091, 1325, 1595, 1700, 2684
MfeI CAATTG 1 cut(s) 3015
MflI RGATCY 2 cut(s) 1300, 1473
MhlI GDGCHC 5 cut(s) 1218, 2117, 2278, 2829, 3109
MlsI TGGCCA 3 cut(s) 2162, 2210, 2482
MluNI TGGCCA 3 cut(s) 2162, 2210, 2482
MlyI GAGTC 4 cut(s) 1294, 2315, 2753, 3033
MmeI TCCRAC 5 cut(s) 234, 2585, 2913, 3047, 3398
Mox20I TGGCCA 3 cut(s) 2162, 2210, 2482
MroXI GAANNNNTTC 2 cut(s) 492, 645
MscI TGGCCA 3 cut(s) 2162, 2210, 2482
MseI TTAA 9 cut(s) 288, 509, 1361, 1592, 1722, 1751, 2202, 2790, 3168
MslI CAYNNNNRTG 2 cut(s) 1829, 3160
Msp20I TGGCCA 3 cut(s) 2162, 2210, 2482
MspA1I CMGCKG 3 cut(s) 1153, 1193, 1351
MspCI CTTAAG 1 cut(s) 1591
MspI CCGG 7 cut(s) 686, 833, 1275, 1438, 1995, 2437, 3179
MspR9I CCNGG 4 cut(s) 686, 1996, 2438, 3179
MunI CAATTG 1 cut(s) 3015
NciI CCSGG 4 cut(s) 686, 1996, 2438, 3179
NheI GCTAGC 1 cut(s) 307
NlaIV GGNNCC 6 cut(s) 218, 1398, 1493, 2563, 2609, 3280
NmuCI GTSAC 1 cut(s) 277
NsbI TGCGCA 1 cut(s) 2733
NspI RCATGY 2 cut(s) 784, 2644
PaeI GCATGC 1 cut(s) 784
PagI TCATGA 2 cut(s) 2125, 2671
PaqCI CACCTGC 1 cut(s) 706
PdmI GAANNNNTTC 2 cut(s) 492, 645
PfeI GAWTC 7 cut(s) 571, 826, 1461, 1672, 1690, 2303, 2531
PflFI GACNNNGTC 1 cut(s) 793
PflMI CCANNNNNTGG 1 cut(s) 3049
PfoI TCCNGGA 1 cut(s) 684
PleI GAGTC 4 cut(s) 1293, 2315, 2753, 3033
PpsI GAGTC 4 cut(s) 1293, 2315, 2753, 3033
PpuMI RGGWCCY 1 cut(s) 499
PshAI GACNNNNGTC 1 cut(s) 2077
PshBI ATTAAT 1 cut(s) 288
PsiI TTATAA 1 cut(s) 2003
Psp124BI GAGCTC 1 cut(s) 1218
Psp5II RGGWCCY 1 cut(s) 499
PspFI CCCAGC 1 cut(s) 1189
PspN4I GGNNCC 6 cut(s) 218, 1398, 1493, 2563, 2609, 3280
PspPI GGNCC 6 cut(s) 246, 499, 1397, 2813, 2883, 3279
PspPPI RGGWCCY 1 cut(s) 499
PsrI GAACNNNNNNTAC 2 cut(s) 2012, 2044
PstI CTGCAG 4 cut(s) 789, 995, 1158, 3141
PstNI CAGNNNCTG 2 cut(s) 502, 793
PsuI RGATCY 2 cut(s) 1300, 1473
PsyI GACNNNGTC 1 cut(s) 793
PvuII CAGCTG 3 cut(s) 1153, 1193, 1351
RseI CAYNNNNRTG 2 cut(s) 1829, 3160
SacI GAGCTC 1 cut(s) 1218
SaqAI TTAA 9 cut(s) 288, 509, 1361, 1592, 1722, 1751, 2202, 2790, 3168
Sau96I GGNCC 6 cut(s) 246, 499, 1397, 2813, 2883, 3279
ScaI AGTACT 2 cut(s) 1656, 2721
SchI GAGTC 4 cut(s) 1294, 2315, 2753, 3033
ScrFI CCNGG 4 cut(s) 686, 1996, 2438, 3179
SduI GDGCHC 5 cut(s) 1218, 2117, 2278, 2829, 3109
SfaNI GCATC 1 cut(s) 2408
SfcI CTRYAG 5 cut(s) 785, 991, 1154, 2772, 3137
SinI GGWCC 6 cut(s) 246, 499, 1397, 2813, 2883, 3279
SmiMI CAYNNNNRTG 2 cut(s) 1829, 3160
SmlI CTYRAG 2 cut(s) 1073, 1591
SmoI CTYRAG 2 cut(s) 1073, 1591
SphI GCATGC 1 cut(s) 784
SspMI CTAG 9 cut(s) 57, 308, 1298, 1623, 1949, 2118, 2169, 2390, 2990
SstI GAGCTC 1 cut(s) 1218
StyD4I CCNGG 4 cut(s) 684, 1994, 2436, 3177
StyI CCWWGG 3 cut(s) 1606, 2330, 2557
TaaI ACNGT 8 cut(s) 449, 848, 1222, 1532, 1706, 1848, 2076, 2896
TaiI ACGT 4 cut(s) 116, 150, 438, 672
TaqI TCGA 6 cut(s) 78, 649, 797, 1248, 2071, 2272
TaqII GACCGA 1 cut(s) 2871
TatI WGTACW 5 cut(s) 1424, 1654, 2637, 2719, 2967
TfiI GAWTC 7 cut(s) 571, 826, 1461, 1672, 1690, 2303, 2531
Tru1I TTAA 9 cut(s) 288, 509, 1361, 1592, 1722, 1751, 2202, 2790, 3168
Tru9I TTAA 9 cut(s) 288, 509, 1361, 1592, 1722, 1751, 2202, 2790, 3168
TscAI CASTG 5 cut(s) 286, 1327, 1471, 1831, 2047
TseFI GTSAC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 277
TspGWI ACGGA 1 cut(s) 1727
TspRI CASTG 5 cut(s) 286, 1327, 1471, 1831, 2047
Tth111I GACNNNGTC 1 cut(s) 793
Van91I CCANNNNNTGG 1 cut(s) 3049
Vha464I CTTAAG 1 cut(s) 1591
VpaK11BI GGWCC 6 cut(s) 246, 499, 1397, 2813, 2883, 3279
VspI ATTAAT 1 cut(s) 288
XapI RAATTY 3 cut(s) 328, 1024, 1357
XbaI TCTAGA 3 cut(s) 1297, 2117, 2168
XceI RCATGY 2 cut(s) 784, 2644
XmnI GAANNNNTTC 2 cut(s) 492, 645
XspI CTAG 9 cut(s) 57, 308, 1298, 1623, 1949, 2118, 2169, 2390, 2990
ZrmI AGTACT 2 cut(s) 1656, 2721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.