MD10G1169800.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013816
Physical Location & Seq
Forward (+)
26209576 .. 26213430
3855 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1169800.v1.1.491

Sequence Viewer

Length: 3486 bp
ATGCCAATGCTGAGTAGCATGCACTCCTCTCTCCTCTCCTACAACCACCACCAAATGCCGAGGCAATCCTTCACCACTCGCTGCTACCACCACCACCACCACCACCACTTGCTCTTTTCTCTTCTCGTCTTCCTCTTGCTATCCACACTTTTACCACTTTCAGCTACAAACATTGAGGTTGTCACACTACATTCATGGCTTCAAACCTCTACCTTGCCCCCGCCGTCATCATCGGATTTCTCCAACTGGAACCCTAAAGACCCCAACCCTTGTAGTTGGTCATACATAACCTGTTCTCCTCAAAACTTTGTCACCGAAATCAATATCCAATCCCTTGAGCTAGCCCTTCCCTTTCCTTCCAATCTCTCCTCCCTTGCCTTCCTCCAAAAGCTCATCATTTCTGGTGCTAACCTCACCGGGACACTCTCACCCGATATCGGATACTGCAATGCACTTACTGTCATTGATGTTAGCTCAAACAGTCTTGTGGCAGAAATTCCTTCAACCATTGGAAAGCTGCAAAATCTTCAGGACTTGATTTTAAATTCTAACCAACTCACTGGGCAGATACCAAAAGAGCTTGGTGATTGCACCAGCCTGAAGAATCTCCACGTATTTGACAACTATCTCTCCGGGAGTATTCCTGCCGAGCTTGGCAAACTAGTAAACTTGGAAGTTATTCGTGCCGGTGGGAACAAAGACATTTCAGGAAAAATCCCTGATGAGCTTGGAAACTGCAAGAACTTGCTGGTGCTTGGCCTTGCTGATACTAAAGTTTCCGGCTCCTTACCTACTTCATTAGGTAAACTACGCATGCTGCAAACTCTGTCTGTTTATACCACAATGATTTCGGGTGAGATTCCGCCGGAGATTGGCAACTGTTCGGAGCTTGTGAACTTGTTTTTGTACGAGAATGATCTGTCCGGTTCACTACCATCAGAGTTAGGTAAACTTCAGAAGTTGGAGAAGATGTTACTGTGGCAAAACAATCTTGATGGTACTATTCCTGAGGAAATTGGTAACTGCAGAAGCTTGATGACCATAGATCTCTCTTTGAATTCTGTCTCTGGAAGCATACCACAATCATTCGGGAACCTCTCGAATCTTCAAGAGCTTATGCTTAGCAACAATAACATTTCTGGTTCAATCCCATCGGTTCTTTCCAATGCTACAAAACTCTTGCAGCTTCAGCTCGATACTAATCAGATTTCGGGCTTAATCCCAACTCAGTTTGGAATGTTGCCAGAGCTGACAGTGTTTTTCGCTTGGCAGAACAAACTCGAAGGCAGCATTCCATCCGAATTGGCTAGCTGTAAGAGCCTTCAGGCTCTTGATCTCTCACACAATGCTCTCACCGGTAGCTTACCTCCTGGCCTATTTCAGCTCCAAAATTTAACCAAGCTTCTATTGATTTCTAATGAACTTTCCGGTTCCATACCTTCTGATATTGGAAATTGCAGCTCTCTTATCCGTCTCCGCCTTGTAAACAACAGAATCAGTGGAGAGATCCCAAAAGAAATTGGGTTTCTCGACAACCTTAGCTTCCTTGACCTGTCCGAAAACAATCTTGTTGGTCCGCTGCCTGATGAAATTGGAAAATGCAGTTCACTGCAGCTGCTGAACTTAAGTAACAATACCCTTGGAGGTACATTGCCTAGCCTTTTCTCTTCCCTCACACGGCTTGAGGTACTGGATGTCTCTGTGAATCAAATTTCGGGTCAGATTCCCGAGAGTTATGGCAAGCTTGCTTCACTTAACCGGCTCATCCTCAGCGGGAACTCCCTCTCTGGACCGATCCCCTCTTCCCTTGGTCATTGTTCAAGCCTTCAGCTACTTGATCTTAGCAGCAACAAGCTCAGTGGCGCAATCCCCGAGGACTTGTTTGAGATTGAAGCTCTTGACATTGCTCTAAACCTGAGTTTCAATGCCTTATCCGGTGTAATCCCACCACAGATTTCTGCTCTGAACAAGCTGTCCATACTAGACCTTTCACACAACAAGCTTGAAGGTGACTTGTTGGTGTTATCCGGTCTTGCAAATCTTGTTTCCTTGAACATCTCATATAACAATTTTACCGGCTATCTCCCCGATGAAAAGCTCTTTCGACAGTTGTCACCAACGGACCTGGCAGGAAACAAAGGGTTGTGCTCTAGAGGCCATGACCAATGTTTCCTCAGCAATGGGACCACAACGAGCATGACAAATAGCGGCCGTTTCAGAAGGTCATGGAGGCTTAAGTTAGCCATTGGATTGTTGATTGCCTTTACCATTGCCTTGGCAATTTTCGGGGCTGTTGCAGTGTATCGAACGCGAAAGATGATGGGAGACGAAAACGATTCTGAGATGGGAGGGGACTCGTGGCCTTGGCAGTTTACTCCCTTCCAGAAGGTAAATTTTTCGGTGGATCAAGTACTAAAATGTCTGGTGGAAACCAATGTAATAGGAAAGGGATGTTCAGGAGTTGTTTACCGGGCGGAAATGGAGAACGAGGACATTGCAGTAAAGAAGCTCTGGCCAGCAAAGATCACTACTAAATATGACTGTCAACGTGACAGGTTTGGAATCAATGCAGGTGTTCGCGATTCCTTTTCAGCTGAAGTGAAGACCCTTGGCTCAATTCGCCACAAGAACATTGTTAGGTTCTTGGGTTGCTGTTGGAATCGAAACACGAGACTGCTAATGTATGAGTACATGTCGAATGGGAGCTTGGGCGGTTTACTTCACGAAAGAAGTGGTAACTGCTTGGAATGGGAATTGAGATATCGGATTGTGCTGGGAGCGGCACAAGGCTTGGCCTACTTGCATCACGATTGCGTTCCTCCGATTGTTCACAGGGACATCAAGGCCAATAACATCCTCATTGGTCCGGACTTTGAGCCCTGCATTGCTGATTTCGGGCTTGCCAAGCTTGTTGATGAAGGAGATTTTGCTCGATCCTCCAACACAGTTGCTGGTTCTTATGGCTACATTGCTCCTGAATATGGATACATGATGAAGATAACAGAGAAAAGTGACGTATACAGCTACGGTGTAGTTGTGTTAGAAGTACTAACAGGGAAACAACCGATCGATCCAACCATACCAGACGGGTTGCACATTGTAGATTGGGTTAGGCAGAAGAGAGGGGGAGTTGAAGTCCTCGATCAAAGCCTACTTGCCCGGCCAGAGTCCGAAATTGAAGAAATGCTGCAGACTCTAGGTGTGGCATTGCTCTGCATAAACTCAAACCCAGATGACAGACCAACCATGAAAGACGTAGCGGCAATGCTCAAAGAAATAAGGCAGGAGAGGGAGGAATGCATGAAAGCTAACATGCTTCGAAACGGATCTTCTGAAAATGGTGGACAAGAAAATAACAGTGGAGGAAGCTCATCGGCTATGGCTGTGCAGCAACATTCGTACCCTTCGAGTACCAGCACAAGTTTCTCAGCATCCTCATTGCTATACACTTCTCCTCCTCCTAATGTCCGAAAAAGTTCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1162

Amino Acids

126.62

Weight (kDa)

5.58

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 121 - 228 3.4e-09 Leucine-rich repeat region
LRR_8 PF13855 152 - 210 5.5e-06 Leucine rich repeat
LRR_14 PF23598 241 - 328 4e-10 Leucine-rich repeat region
LRR_8 PF13855 320 - 379 2.8e-07 Leucine rich repeat
LRR_14 PF23598 334 - 419 8.2e-06 Leucine-rich repeat region
LRR_8 PF13855 343 - 403 1.9e-07 Leucine rich repeat
LRR_14 PF23598 492 - 705 5.3e-15 Leucine-rich repeat region
LRR_8 PF13855 536 - 595 3.8e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 811 - 1089 7.3e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 811 - 1087 3.6e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48940
fragaria_vesca FvH4_2g19620
malus_domestica MD10G1169800.v1.1
prunus_persica Prupe.8G207600_v2.0.a1
pyrus_communis pycom10g14630
rosa_chinensis RchiOBHm_Chr6g0285351
rosa_laevigata RLG00000012651
rosa_multiflora Rmu_co8152086.1_g000001 Rmu_co8221716.1_g000001 Rmu_sc0012287.1_g000004
rosa_roxburghii Rroxscaffold_7G00183330
rosa_rugosa Rorug06G0173900
rosa_samantha Rh6AG284500 Rh6BG285700 Rh6CG286800 Rh6DG279800
rosa_wichuraiana Rw6G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2561
Acc36I ACCTGC 1 cut(s) 2561
AccBSI CCGCTC 1 cut(s) 2780
AccI GTMKAC 1 cut(s) 3018
AccII CGCG 2 cut(s) 2311, 2580
AccIII TCCGGA 1 cut(s) 2866
AclWI GGATC 6 cut(s) 1501, 1789, 2412, 2928, 3065, 3334
AcoI YGGCCR 3 cut(s) 2209, 2513, 3161
AcsI RAATTY 6 cut(s) 495, 544, 1057, 1390, 1710, 2392
AcuI CTGAAG 7 cut(s) 512, 620, 938, 1172, 1307, 1811, 2616
AfaI GTAC 9 cut(s) 908, 1000, 1648, 1689, 2412, 2690, 3048, 3401, 3412
AfiI CCNNNNNNNGG 4 cut(s) 437, 872, 1677, 2981
AflII CTTAAG 2 cut(s) 1624, 2234
AflIII ACRYGT 1 cut(s) 2691
AgeI ACCGGT 1 cut(s) 1355
AhlI ACTAGT 1 cut(s) 661
AjnI CCWGG 2 cut(s) 1369, 2124
AjuI GAANNNNNNNTTGG 2 cut(s) 2690, 2722
AloI GAACNNNNNNTCC 2 cut(s) 1958, 1990
Alw21I GWGCWC 1 cut(s) 2150
Alw26I GTCTC 5 cut(s) 1069, 1478, 1702, 2319, 2665
AlwI GGATC 6 cut(s) 1501, 1789, 2412, 2928, 3065, 3334
AlwNI CAGNNNCTG 2 cut(s) 1618, 2951
Ama87I CYCGRG 2 cut(s) 1727, 1871
Aor13HI TCCGGA 1 cut(s) 2866
AoxI GGCC 9 cut(s) 757, 1372, 2155, 2209, 2360, 2513, 2793, 2844, 3161
ApoI RAATTY 6 cut(s) 495, 544, 1057, 1390, 1710, 2392
ArsI GACNNNNNNTTYG 2 cut(s) 1702, 1734
AsiGI ACCGGT 1 cut(s) 1355
Asp700I GAANNNNTTC 2 cut(s) 678, 3475
AspLEI GCGC 1 cut(s) 1865
AspS9I GGNCC 5 cut(s) 1574, 1790, 2122, 2184, 2864
AsuC2I CCSGG 4 cut(s) 418, 634, 2471, 3160
AsuHPI GGTGA 9 cut(s) 64, 304, 406, 420, 596, 866, 1345, 2021, 2106
AsuII TTCGAA 1 cut(s) 3319
AsuNHI GCTAGC 2 cut(s) 340, 1307
AvaI CYCGRG 2 cut(s) 1727, 1871
AvaII GGWCC 5 cut(s) 1574, 1790, 2122, 2184, 2864
AxyI CCTNAGG 1 cut(s) 1008
BaeI ACNNNNGTAYC 4 cut(s) 759, 792, 3383, 3416
BalI TGGCCA 1 cut(s) 2515
BanII GRGCYC 1 cut(s) 2880
BauI CACGAG 2 cut(s) 2356, 2668
BbsI GAAGAC 2 cut(s) 121, 2609
Bbv12I GWGCWC 1 cut(s) 2150
BbvCI CCTCAGC 2 cut(s) 1769, 2174
BccI CCATC 6 cut(s) 943, 989, 1159, 1303, 2314, 2338
BceAI ACGGC 3 cut(s) 208, 1694, 2196
BcgI CGANNNNNNTGC 2 cut(s) 2477, 2511
BciT130I CCWGG 2 cut(s) 1371, 2126
BciVI GTATCC 2 cut(s) 434, 2978
BcnI CCSGG 4 cut(s) 418, 634, 2471, 3160
BcoDI GTCTC 5 cut(s) 1069, 1478, 1702, 2319, 2665
BcuI ACTAGT 1 cut(s) 661
BfaI CTAG 7 cut(s) 341, 662, 1308, 1656, 1982, 2151, 3197
BfmI CTRYAG 3 cut(s) 1024, 1610, 3188
BfrI CTTAAG 2 cut(s) 1624, 2234
BfuAI ACCTGC 1 cut(s) 2561
BfuI GTATCC 2 cut(s) 434, 2978
BglII AGATCT 1 cut(s) 1045
BlpI GCTNAGC 1 cut(s) 1121
BmcAI AGTACT 2 cut(s) 2412, 3048
Bme1390I CCNGG 6 cut(s) 418, 634, 1371, 2126, 2471, 3160
Bme18I GGWCC 5 cut(s) 1574, 1790, 2122, 2184, 2864
BmeT110I CYCGRG 2 cut(s) 1727, 1871
BmgT120I GGNCC 5 cut(s) 1574, 1790, 2122, 2184, 2864
BmiI GGNNCC 5 cut(s) 251, 784, 1094, 1432, 2185
BmrFI CCNGG 6 cut(s) 418, 634, 1371, 2126, 2471, 3160
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 2 cut(s) 2812, 3440
BmtI GCTAGC 2 cut(s) 344, 1311
BmuI ACTGGG 1 cut(s) 570
BoxI GACNNNNGTC 1 cut(s) 2110
BpiI GAAGAC 2 cut(s) 121, 2609
Bpu10I CCTNAGC 3 cut(s) 1538, 1769, 2174
Bpu1102I GCTNAGC 1 cut(s) 1121
Bpu14I TTCGAA 1 cut(s) 3319
BpuEI CTTGAG 2 cut(s) 356, 1703
BpuMI CCSGG 4 cut(s) 418, 634, 2471, 3160
Bsa29I ATCGAT 1 cut(s) 3069
BsaAI YACGTR 1 cut(s) 613
BsaBI GATNNNNATC 1 cut(s) 1200
BsaJI CCNNGG 7 cut(s) 59, 1639, 1807, 1872, 2274, 2363, 2608
BsaWI WCCGGW 6 cut(s) 923, 1355, 1427, 1934, 2027, 2866
Bsc4I CCNNNNNNNGG 4 cut(s) 437, 872, 1677, 2981
Bse118I RCCGGY 4 cut(s) 686, 1355, 1758, 2075
Bse1I ACTGG 3 cut(s) 251, 565, 1695
Bse21I CCTNAGG 1 cut(s) 1008
Bse8I GATNNNNATC 1 cut(s) 1200
BseAI TCCGGA 1 cut(s) 2866
BseBI CCWGG 2 cut(s) 1371, 2126
BseCI ATCGAT 1 cut(s) 3069
BseDI CCNNGG 7 cut(s) 59, 1639, 1807, 1872, 2274, 2363, 2608
BseGI GGATG 6 cut(s) 1295, 1699, 1764, 2456, 2853, 3431
BseJI GATNNNNATC 1 cut(s) 1200
BseLI CCNNNNNNNGG 4 cut(s) 437, 872, 1677, 2981
BseMII CTCAG 8 cut(s) 999, 1241, 1783, 1870, 1907, 2188, 2331, 3441
BseNI ACTGG 3 cut(s) 251, 565, 1695
BseRI GAGGAG 6 cut(s) 16, 23, 288, 358, 3444, 3447
BseX3I CGGCCG 1 cut(s) 2209
BseYI CCCAGC 1 cut(s) 2773
BsgI GTGCAG 1 cut(s) 3407
Bsh1236I CGCG 2 cut(s) 2311, 2580
Bsh1285I CGRYCG 2 cut(s) 2212, 3069
BshFI GGCC 9 cut(s) 759, 1374, 2157, 2211, 2362, 2515, 2795, 2846, 3163
BshTI ACCGGT 1 cut(s) 1355
BshVI ATCGAT 1 cut(s) 3069
BsiEI CGRYCG 2 cut(s) 2212, 3069
BsiHKAI GWGCWC 1 cut(s) 2150
BsiHKCI CYCGRG 2 cut(s) 1727, 1871
BslFI GGGAC 4 cut(s) 433, 2197, 2366, 2849
BslI CCNNNNNNNGG 4 cut(s) 437, 872, 1677, 2981
BsmAI GTCTC 5 cut(s) 1069, 1478, 1702, 2319, 2665
BsmBI CGTCTC 2 cut(s) 1478, 2319
BsmFI GGGAC 4 cut(s) 433, 2197, 2366, 2849
BsmI GAATGC 2 cut(s) 1290, 3302
BsnI GGCC 9 cut(s) 759, 1374, 2157, 2211, 2362, 2515, 2795, 2846, 3163
BsoBI CYCGRG 2 cut(s) 1727, 1871
Bsp119I TTCGAA 1 cut(s) 3319
Bsp1286I GDGCHC 2 cut(s) 2150, 2880
Bsp13I TCCGGA 1 cut(s) 2866
Bsp1720I GCTNAGC 1 cut(s) 1121
Bsp68I TCGCGA 1 cut(s) 2580
BspANI GGCC 9 cut(s) 759, 1374, 2157, 2211, 2362, 2515, 2795, 2846, 3163
BspCNI CTCAG 8 cut(s) 1000, 1240, 1782, 1869, 1908, 2187, 2332, 3440
BspDI ATCGAT 1 cut(s) 3069
BspEI TCCGGA 1 cut(s) 2866
BspFNI CGCG 2 cut(s) 2311, 2580
BspLI GGNNCC 5 cut(s) 251, 784, 1094, 1432, 2185
BspMAI CTGCAG 3 cut(s) 1028, 1614, 3192
BspMI ACCTGC 1 cut(s) 2561
BspOI GCTAGC 2 cut(s) 344, 1311
BspPI GGATC 6 cut(s) 1501, 1789, 2412, 2928, 3065, 3334
BspT104I TTCGAA 1 cut(s) 3319
BspTI CTTAAG 2 cut(s) 1624, 2234
BsrBI CCGCTC 1 cut(s) 2780
BsrFI RCCGGY 4 cut(s) 686, 1355, 1758, 2075
BsrI ACTGG 3 cut(s) 251, 565, 1695
BssAI RCCGGY 4 cut(s) 686, 1355, 1758, 2075
BssECI CCNNGG 7 cut(s) 59, 1639, 1807, 1872, 2274, 2363, 2608
BssNAI GTATAC 1 cut(s) 3019
BssSI CACGAG 2 cut(s) 2356, 2668
BssT1I CCWWGG 5 cut(s) 1639, 1807, 2274, 2363, 2608
Bst1107I GTATAC 1 cut(s) 3019
Bst2BI CACGAG 2 cut(s) 2356, 2668
Bst2UI CCWGG 2 cut(s) 1371, 2126
Bst6I CTCTTC 4 cut(s) 126, 1672, 1807, 3113
BstAFI CTTAAG 2 cut(s) 1624, 2234
BstBAI YACGTR 1 cut(s) 613
BstBI TTCGAA 1 cut(s) 3319
BstC8I GCNNGC 8 cut(s) 20, 342, 815, 1309, 1742, 1746, 2517, 2901
BstF5I GGATG 6 cut(s) 1295, 1699, 1764, 2456, 2853, 3431
BstFNI CGCG 2 cut(s) 2311, 2580
BstHHI GCGC 1 cut(s) 1865
BstMAI GTCTC 5 cut(s) 1069, 1478, 1702, 2319, 2665
BstMCI CGRYCG 2 cut(s) 2212, 3069
BstMWI GCNNNNNNNGC 5 cut(s) 1189, 1317, 2154, 2619, 2905
BstNI CCWGG 2 cut(s) 1371, 2126
BstNSI RCATGY 4 cut(s) 22, 817, 2695, 3316
BstPAI GACNNNNGTC 1 cut(s) 2110
BstSCI CCNGG 6 cut(s) 416, 632, 1369, 2124, 2469, 3158
BstSFI CTRYAG 3 cut(s) 1024, 1610, 3188
BstUI CGCG 2 cut(s) 2311, 2580
BstV2I GAAGAC 2 cut(s) 121, 2609
BstX2I RGATCY 3 cut(s) 1045, 1506, 3326
BstXI CCANNNNNNTGG 2 cut(s) 560, 2275
BstYI RGATCY 3 cut(s) 1045, 1506, 3326
BstZ17I GTATAC 1 cut(s) 3019
BstZI CGGCCG 1 cut(s) 2209
Bsu15I ATCGAT 1 cut(s) 3069
Bsu36I CCTNAGG 1 cut(s) 1008
BsuI GTATCC 2 cut(s) 434, 2978
BsuRI GGCC 9 cut(s) 759, 1374, 2157, 2211, 2362, 2515, 2795, 2846, 3163
BsuTUI ATCGAT 1 cut(s) 3069
BtsCI GGATG 6 cut(s) 1295, 1699, 1764, 2456, 2853, 3431
BtsI GCAGTG 2 cut(s) 1607, 2304
BtsIMutI CAGTG 7 cut(s) 558, 1260, 1504, 1607, 1864, 2304, 3364
BtuMI TCGCGA 1 cut(s) 2580
BveI ACCTGC 1 cut(s) 2561
Cac8I GCNNGC 8 cut(s) 20, 342, 815, 1309, 1742, 1746, 2517, 2901
CaiI CAGNNNCTG 2 cut(s) 1618, 2951
CfoI GCGC 1 cut(s) 1865
Cfr10I RCCGGY 4 cut(s) 686, 1355, 1758, 2075
Cfr13I GGNCC 5 cut(s) 1574, 1790, 2122, 2184, 2864
ClaI ATCGAT 1 cut(s) 3069
Csp6I GTAC 9 cut(s) 907, 999, 1647, 1688, 2411, 2689, 3047, 3400, 3411
CspAI ACCGGT 1 cut(s) 1355
CspCI CAANNNNNGTGG 2 cut(s) 1840, 1875
CviQI GTAC 9 cut(s) 907, 999, 1647, 1688, 2411, 2689, 3047, 3400, 3411
DraI TTTAAA 1 cut(s) 543
EaeI YGGCCR 3 cut(s) 2209, 2513, 3161
EagI CGGCCG 1 cut(s) 2209
Eam1104I CTCTTC 4 cut(s) 126, 1672, 1807, 3113
EarI CTCTTC 4 cut(s) 126, 1672, 1807, 3113
EciI GGCGGA 3 cut(s) 852, 1466, 2489
EclXI CGGCCG 1 cut(s) 2209
Eco130I CCWWGG 5 cut(s) 1639, 1807, 2274, 2363, 2608
Eco24I GRGCYC 1 cut(s) 2880
Eco32I GATATC 2 cut(s) 436, 2762
Eco47I GGWCC 5 cut(s) 1574, 1790, 2122, 2184, 2864
Eco52I CGGCCG 1 cut(s) 2209
Eco57I CTGAAG 7 cut(s) 512, 620, 938, 1172, 1307, 1811, 2616
Eco81I CCTNAGG 1 cut(s) 1008
Eco88I CYCGRG 2 cut(s) 1727, 1871
EcoRI GAATTC 1 cut(s) 1057
EcoRII CCWGG 2 cut(s) 1369, 2124
EcoRV GATATC 2 cut(s) 436, 2762
EcoT14I CCWWGG 5 cut(s) 1639, 1807, 2274, 2363, 2608
EcoT22I ATGCAT 1 cut(s) 3302
EcoT38I GRGCYC 1 cut(s) 2880
ErhI CCWWGG 5 cut(s) 1639, 1807, 2274, 2363, 2608
Esp3I CGTCTC 2 cut(s) 1478, 2319
FaqI GGGAC 4 cut(s) 433, 2197, 2366, 2849
FauI CCCGC 2 cut(s) 228, 1766
FblI GTMKAC 1 cut(s) 3018
FokI GGATG 6 cut(s) 1282, 1706, 1751, 2463, 2840, 3418
FriOI GRGCYC 1 cut(s) 2880
FspBI CTAG 7 cut(s) 341, 662, 1308, 1656, 1982, 2151, 3197
GlaI GCGC 1 cut(s) 1864
GsaI CCCAGC 1 cut(s) 2777
HaeIII GGCC 9 cut(s) 759, 1374, 2157, 2211, 2362, 2515, 2795, 2846, 3163
HhaI GCGC 1 cut(s) 1865
Hin6I GCGC 1 cut(s) 1863
HinP1I GCGC 1 cut(s) 1863
HincII GTYRAC 1 cut(s) 2546
HindII GTYRAC 1 cut(s) 2546
HindIII AAGCTT 5 cut(s) 1030, 1400, 1742, 2000, 2906
HphI GGTGA 9 cut(s) 64, 304, 406, 420, 596, 866, 1345, 2021, 2106
HpyCH4IV ACGT 4 cut(s) 612, 2548, 3015, 3255
HpyF10VI GCNNNNNNNGC 5 cut(s) 1189, 1317, 2154, 2619, 2905
HpySE526I ACGT 4 cut(s) 612, 2548, 3015, 3255
HspAI GCGC 1 cut(s) 1863
Kpn2I TCCGGA 1 cut(s) 2866
LmnI GCTCC 6 cut(s) 788, 886, 1389, 2703, 2777, 2977
LweI GCATC 2 cut(s) 2812, 3440
MaeI CTAG 7 cut(s) 341, 662, 1308, 1656, 1982, 2151, 3197
MaeII ACGT 4 cut(s) 612, 2548, 3015, 3255
MbiI CCGCTC 1 cut(s) 2780
MflI RGATCY 3 cut(s) 1045, 1506, 3326
MhlI GDGCHC 2 cut(s) 2150, 2880
MlsI TGGCCA 1 cut(s) 2515
MluNI TGGCCA 1 cut(s) 2515
MlyI GAGTC 3 cut(s) 2348, 3176, 3187
MmeI TCCRAC 5 cut(s) 267, 942, 2636, 2964, 3098
Mox20I TGGCCA 1 cut(s) 2515
Mph1103I ATGCAT 1 cut(s) 3302
MroI TCCGGA 1 cut(s) 2866
MroXI GAANNNNTTC 2 cut(s) 678, 3475
MscI TGGCCA 1 cut(s) 2515
MseI TTAA 6 cut(s) 542, 1217, 1394, 1625, 1755, 2235
Msp20I TGGCCA 1 cut(s) 2515
MspA1I CMGCKG 4 cut(s) 1579, 1615, 1773, 2594
MspCI CTTAAG 2 cut(s) 1624, 2234
MspR9I CCNGG 6 cut(s) 418, 634, 1371, 2126, 2471, 3160
Mva1269I GAATGC 2 cut(s) 1290, 3302
MvaI CCWGG 2 cut(s) 1371, 2126
MvnI CGCG 2 cut(s) 2311, 2580
MwoI GCNNNNNNNGC 5 cut(s) 1189, 1317, 2154, 2619, 2905
NciI CCSGG 4 cut(s) 418, 634, 2471, 3160
NheI GCTAGC 2 cut(s) 340, 1307
NlaIV GGNNCC 5 cut(s) 251, 784, 1094, 1432, 2185
NmeAIII GCCGAG 2 cut(s) 84, 673
NmuCI GTSAC 6 cut(s) 181, 310, 2009, 2112, 2549, 3011
NruI TCGCGA 1 cut(s) 2580
NsiI ATGCAT 1 cut(s) 3302
NspI RCATGY 4 cut(s) 22, 817, 2695, 3316
NspV TTCGAA 1 cut(s) 3319
PaeI GCATGC 2 cut(s) 22, 817
PaqCI CACCTGC 1 cut(s) 2561
PciI ACATGT 1 cut(s) 2691
PcsI WCGNNNNNNNCGW 1 cut(s) 3404
PctI GAATGC 2 cut(s) 1290, 3302
PdmI GAANNNNTTC 2 cut(s) 678, 3475
PfoI TCCNGGA 1 cut(s) 632
PinAI ACCGGT 1 cut(s) 1355
Ple19I CGATCG 1 cut(s) 3069
PleI GAGTC 3 cut(s) 2348, 3175, 3187
PpsI GAGTC 3 cut(s) 2348, 3175, 3187
Ppu21I YACGTR 1 cut(s) 613
PscI ACATGT 1 cut(s) 2691
PshAI GACNNNNGTC 1 cut(s) 2110
Psp6I CCWGG 2 cut(s) 1369, 2124
PspFI CCCAGC 1 cut(s) 2773
PspGI CCWGG 2 cut(s) 1369, 2124
PspN4I GGNNCC 5 cut(s) 251, 784, 1094, 1432, 2185
PspPI GGNCC 5 cut(s) 1574, 1790, 2122, 2184, 2864
PstI CTGCAG 3 cut(s) 1028, 1614, 3192
PstNI CAGNNNCTG 2 cut(s) 1618, 2951
PsuI RGATCY 3 cut(s) 1045, 1506, 3326
PvuI CGATCG 1 cut(s) 3069
PvuII CAGCTG 2 cut(s) 1615, 2594
RruI TCGCGA 1 cut(s) 2580
RsaI GTAC 9 cut(s) 908, 1000, 1648, 1689, 2412, 2690, 3048, 3401, 3412
RsaNI GTAC 9 cut(s) 907, 999, 1647, 1688, 2411, 2689, 3047, 3400, 3411
SaqAI TTAA 6 cut(s) 542, 1217, 1394, 1625, 1755, 2235
Sau96I GGNCC 5 cut(s) 1574, 1790, 2122, 2184, 2864
ScaI AGTACT 2 cut(s) 2412, 3048
SchI GAGTC 3 cut(s) 2348, 3176, 3187
ScrFI CCNGG 6 cut(s) 418, 634, 1371, 2126, 2471, 3160
SduI GDGCHC 2 cut(s) 2150, 2880
SfaNI GCATC 2 cut(s) 2812, 3440
SfcI CTRYAG 3 cut(s) 1024, 1610, 3188
SfuI TTCGAA 1 cut(s) 3319
SinI GGWCC 5 cut(s) 1574, 1790, 2122, 2184, 2864
SmlI CTYRAG 4 cut(s) 335, 1624, 1682, 2234
SmoI CTYRAG 4 cut(s) 335, 1624, 1682, 2234
SpeI ACTAGT 1 cut(s) 661
SphI GCATGC 2 cut(s) 22, 817
SspMI CTAG 7 cut(s) 341, 662, 1308, 1656, 1982, 2151, 3197
StyD4I CCNGG 6 cut(s) 416, 632, 1369, 2124, 2469, 3158
StyI CCWWGG 5 cut(s) 1639, 1807, 2274, 2363, 2608
TaiI ACGT 4 cut(s) 615, 2551, 3018, 3258
TaqII GACCGA 1 cut(s) 1807
TatI WGTACW 3 cut(s) 2410, 2688, 3046
TauI GCSGC 3 cut(s) 2211, 2783, 3263
Tru1I TTAA 6 cut(s) 542, 1217, 1394, 1625, 1755, 2235
Tru9I TTAA 6 cut(s) 542, 1217, 1394, 1625, 1755, 2235
TscAI CASTG 7 cut(s) 565, 1260, 1504, 1614, 1864, 2304, 3364
TseFI GTSAC 6 cut(s) 181, 310, 2009, 2112, 2549, 3011
Tsp45I GTSAC 6 cut(s) 181, 310, 2009, 2112, 2549, 3011
TspDTI ATGAA 9 cut(s) 183, 786, 1434, 1602, 2106, 2931, 3008, 3263, 3317
TspGWI ACGGA 3 cut(s) 1460, 2135, 3339
TspRI CASTG 7 cut(s) 565, 1260, 1504, 1614, 1864, 2304, 3364
Vha464I CTTAAG 2 cut(s) 1624, 2234
VpaK11BI GGWCC 5 cut(s) 1574, 1790, 2122, 2184, 2864
XapI RAATTY 6 cut(s) 495, 544, 1057, 1390, 1710, 2392
XbaI TCTAGA 1 cut(s) 2150
XceI RCATGY 4 cut(s) 22, 817, 2695, 3316
XmiI GTMKAC 1 cut(s) 3018
XmnI GAANNNNTTC 2 cut(s) 678, 3475
XspI CTAG 7 cut(s) 341, 662, 1308, 1656, 1982, 2151, 3197
ZrmI AGTACT 2 cut(s) 2412, 3048
Zsp2I ATGCAT 1 cut(s) 3302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.