FvH4_2g19930

Serine hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011279
Physical Location & Seq
Reverse (-)
16807341 .. 16810561
3221 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19930.t1

Sequence Viewer

Length: 1275 bp
ATGTCTGCTCATCAGTGGCTGGAAAAGGCGGCAACAGCTCTCAATTCTGGCTTGGCCTTCATAGTTTTCCTCGTTTTTGACCTTCTTGACGCCATTCTCTGCATCCTGTTCAGATATATTGACTACTTGTTTGAAGGGCGGCCGGGCTCTTGTTTTTGTGGTCGCAGCTGGGAAATTGAGCAGGTCAGGAATAATGAAGATGATCACCAACAGCTTTCAGAGACTTTATACCGGAGGAAAAACATTTTCAAGGAATTGGGTCGTCTTGTTCGTGTTGCTAAAAGCTACCACAAAACAAGTGGTGGCAGAGTTGCCAGGTGGTCTGACTGTGGATGTCACTCTTGTGTTTCATGGCTGAATACTGAGGAGCACAACCTACATCTTGTTGTTAAGCAGCCCTCTTCACTAGCCATCACTGAGGATTTGGGAGTACAACAAGCAAGTGAGAATGTGATATTCCTTCACGGGTTTCTTTCTTCTTCCTCATTTTGGGCAGAAACTGTGTTTCCAAACATTTCGGATCCTGTTAGTCGGCATTATAAGTTGTTTGCCATAGATCTATTGGGATTTGGTTCAAGCCCAAAGCCAAGGGACTGTTTGTATACTTTGAAGGATCACTTGGAAATGATTGAGAAATCTGTCATATGTCCCTTCCAGTTAACTTCTTTCCATTTGGTTGCACACTCCATGGGTTGTCTGATTGCAATTGCTTTAGCTGCTAAGCACTCGCACTTGGTAAAATCAATCACTCTTGTAGCACCTCCTTACTTCCCTTCTAAAGATGGAGATAGTTTGAGAGTGCTTCATAGGCTTGCTGAAAGGAAACTATGGCCACTTTTCATGTTTGGAACATCATTTATGTCATGGTATGAGCATCTGGGTCGATGTGTCTGTTTCCTTGTATGCCGCAACCACAGGATATGGGAGAGGATCGTAAAGTTAATCACTTGGAGAAAGGACCTCCATTTTATGACAATGGACTTAAGTAGGCACACCCATCATTCAGCTTGGCACACCATGCACAATGTGATTTGTGGGGGTGCAAAGTTAATGGAAAGCTACTTGGAAGTTATAAGCAAAGCCGGGGTTAAAGTGTTTGTAATCCATGGAGATAGAGACCAGGTTGTGCCAGTAGAGTGCAGCCACAACATTTGGATGGCTGTTCCTGAGGCACAAATTAACATCATAAAAAATGCTGATCATTCTTCTGTGCTCCTTGGCCGTGAGAAAGAGTTCACTCTCTATCTACAGCACGTTTGGGCCGCTTCCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0010224 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

425

Amino Acids

48.53

Weight (kDa)

8.17

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 147 - 402 4.4e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 151 - 254 4.2e-24 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 151 - 406 6.2e-18 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17720 AT5G17780 AT5G17780
fragaria_vesca FvH4_2g19930 FvH4_2g19930 FvH4_2g19930
malus_domestica MD12G1013100.v1.1
prunus_persica Prupe.7G017500_v2.0.a1
pyrus_communis pycom12g01040
rosa_chinensis RchiOBHm_Chr3g0495891
rosa_laevigata RLG00000022582
rosa_multiflora Rmu_sc0012596.1_g000001
rosa_roxburghii Rroxscaffold_6G00389940
rosa_rugosa Rorug03G0276800
rosa_samantha Rh3AG324600 Rh3BG360600 Rh3BG360700 Rh3CG356800 Rh3DG359800
rosa_wichuraiana Rw3G028360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 540, 1073
Acc36I ACCTGC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 602
AciI CCGC 4 cut(s) 29, 139, 907, 1263
AclWI GGATC 4 cut(s) 515, 528, 621, 938
AcoI YGGCCR 3 cut(s) 140, 830, 1219
AcyI GRCGYC 1 cut(s) 90
AdeI CACNNNGTG 1 cut(s) 1027
AfaI GTAC 1 cut(s) 432
AfiI CCNNNNNNNGG 1 cut(s) 489
AflII CTTAAG 1 cut(s) 982
AgsI TTSAA 4 cut(s) 134, 250, 576, 610
AjnI CCWGG 2 cut(s) 314, 1119
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AleI CACNNNNGTG 1 cut(s) 342
AluBI AGCT 7 cut(s) 38, 168, 214, 285, 716, 1007, 1059
AluI AGCT 7 cut(s) 38, 168, 214, 285, 716, 1007, 1059
Alw21I GWGCWC 2 cut(s) 372, 1215
Alw26I GTCTC 2 cut(s) 215, 1110
AlwI GGATC 4 cut(s) 515, 528, 621, 938
AlwNI CAGNNNCTG 2 cut(s) 19, 500
AoxI GGCC 5 cut(s) 54, 140, 830, 1219, 1260
ApeKI GCWGC 4 cut(s) 165, 394, 716, 1140
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
Asp700I GAANNNNTTC 1 cut(s) 1232
AspS9I GGNCC 2 cut(s) 958, 1260
AsuC2I CCSGG 2 cut(s) 144, 1084
AsuHPI GGTGA 1 cut(s) 197
AvaII GGWCC 1 cut(s) 958
AxyI CCTNAGG 1 cut(s) 1167
BalI TGGCCA 1 cut(s) 832
BamHI GGATCC 1 cut(s) 520
BanII GRGCYC 1 cut(s) 149
Bbv12I GWGCWC 2 cut(s) 372, 1215
BbvI GCAGC 4 cut(s) 177, 406, 703, 1152
BccI CCATC 4 cut(s) 419, 776, 1005, 1150
BceAI ACGGC 1 cut(s) 1206
BcgI CGANNNNNNTGC 2 cut(s) 863, 897
BciT130I CCWGG 2 cut(s) 316, 1121
BclI TGATCA 2 cut(s) 202, 1198
BcnI CCSGG 2 cut(s) 144, 1084
BcoDI GTCTC 2 cut(s) 215, 1110
BfaI CTAG 1 cut(s) 407
BfmI CTRYAG 1 cut(s) 1247
BfrI CTTAAG 1 cut(s) 982
BfuAI ACCTGC 1 cut(s) 172
BglII AGATCT 1 cut(s) 556
BisI GCNGC 8 cut(s) 30, 140, 166, 395, 717, 907, 1141, 1263
BlpI GCTNAGC 1 cut(s) 720
BlsI GCNGC 8 cut(s) 31, 141, 167, 396, 718, 908, 1142, 1264
Bme1390I CCNGG 4 cut(s) 144, 316, 1084, 1121
Bme18I GGWCC 1 cut(s) 958
BmgT120I GGNCC 2 cut(s) 958, 1260
BmiI GGNNCC 1 cut(s) 522
BmrFI CCNGG 4 cut(s) 144, 316, 1084, 1121
BmsI GCATC 2 cut(s) 111, 883
Bpu1102I GCTNAGC 1 cut(s) 720
BpuMI CCSGG 2 cut(s) 144, 1084
BsaHI GRCGYC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 1110
BsaJI CCNNGG 5 cut(s) 587, 687, 1083, 1105, 1216
BsaWI WCCGGW 1 cut(s) 231
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse1I ACTGG 2 cut(s) 655, 1130
Bse21I CCTNAGG 1 cut(s) 1167
BseBI CCWGG 2 cut(s) 316, 1121
BseDI CCNNGG 5 cut(s) 587, 687, 1083, 1105, 1216
BseGI GGATG 3 cut(s) 102, 338, 1161
BseLI CCNNNNNNNGG 1 cut(s) 489
BseMII CTCAG 3 cut(s) 354, 408, 1158
BseNI ACTGG 2 cut(s) 655, 1130
BseRI GAGGAG 1 cut(s) 380
BseX3I CGGCCG 1 cut(s) 140
BseXI GCAGC 4 cut(s) 177, 406, 703, 1152
BseYI CCCAGC 1 cut(s) 168
BsgI GTGCAG 1 cut(s) 1159
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 5 cut(s) 56, 142, 832, 1221, 1262
BsiEI CGRYCG 1 cut(s) 143
BsiHKAI GWGCWC 2 cut(s) 372, 1215
BsiSI CCGG 3 cut(s) 143, 232, 1083
BslFI GGGAC 2 cut(s) 605, 633
BslI CCNNNNNNNGG 1 cut(s) 489
BsmAI GTCTC 2 cut(s) 215, 1110
BsmFI GGGAC 2 cut(s) 605, 633
BsnI GGCC 5 cut(s) 56, 142, 832, 1221, 1262
Bso31I GGTCTC 1 cut(s) 1110
Bsp1286I GDGCHC 3 cut(s) 149, 372, 1215
Bsp143I GATC 6 cut(s) 202, 520, 556, 613, 930, 1198
Bsp1720I GCTNAGC 1 cut(s) 720
Bsp19I CCATGG 2 cut(s) 687, 1105
BspACI CCGC 4 cut(s) 29, 139, 907, 1263
BspANI GGCC 5 cut(s) 56, 142, 832, 1221, 1262
BspCNI CTCAG 3 cut(s) 355, 409, 1159
BspLI GGNNCC 1 cut(s) 522
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 4 cut(s) 515, 528, 621, 938
BspTI CTTAAG 1 cut(s) 982
BspTNI GGTCTC 1 cut(s) 1110
BsrI ACTGG 2 cut(s) 655, 1130
BssECI CCNNGG 5 cut(s) 587, 687, 1083, 1105, 1216
BssMI GATC 6 cut(s) 202, 520, 556, 613, 930, 1198
BssNAI GTATAC 1 cut(s) 603
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 4 cut(s) 587, 687, 1105, 1216
Bst1107I GTATAC 1 cut(s) 603
Bst2UI CCWGG 2 cut(s) 316, 1121
Bst4CI ACNGT 3 cut(s) 329, 502, 596
Bst6I CTCTTC 1 cut(s) 406
BstACI GRCGYC 1 cut(s) 90
BstAFI CTTAAG 1 cut(s) 982
BstAPI GCANNNNNTGC 1 cut(s) 1018
BstC8I GCNNGC 1 cut(s) 813
BstDEI CTNAG 4 cut(s) 363, 417, 720, 1167
BstDSI CCRYGG 2 cut(s) 687, 1105
BstF5I GGATG 3 cut(s) 102, 338, 1161
BstKTI GATC 6 cut(s) 205, 523, 559, 616, 933, 1201
BstMAI GTCTC 2 cut(s) 215, 1110
BstMBI GATC 6 cut(s) 202, 520, 556, 613, 930, 1198
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 4 cut(s) 35, 716, 808, 1018
BstNI CCWGG 2 cut(s) 316, 1121
BstSCI CCNGG 4 cut(s) 142, 314, 1082, 1119
BstSFI CTRYAG 1 cut(s) 1247
BstV1I GCAGC 4 cut(s) 177, 406, 703, 1152
BstX2I RGATCY 2 cut(s) 520, 556
BstYI RGATCY 2 cut(s) 520, 556
BstZ17I GTATAC 1 cut(s) 603
BstZI CGGCCG 1 cut(s) 140
Bsu36I CCTNAGG 1 cut(s) 1167
BsuRI GGCC 5 cut(s) 56, 142, 832, 1221, 1262
BtgI CCRYGG 2 cut(s) 687, 1105
BtsCI GGATG 3 cut(s) 102, 338, 1161
BtsIMutI CAGTG 2 cut(s) 20, 414
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 1 cut(s) 813
CaiI CAGNNNCTG 2 cut(s) 19, 500
Cfr13I GGNCC 2 cut(s) 958, 1260
CseI GACGC 1 cut(s) 98
CsiI ACCWGGT 1 cut(s) 1119
Csp6I GTAC 1 cut(s) 431
CspCI CAANNNNNGTGG 4 cut(s) 280, 315, 1133, 1168
CviAII CATG 6 cut(s) 351, 688, 841, 864, 1018, 1106
CviQI GTAC 1 cut(s) 431
DdeI CTNAG 4 cut(s) 363, 417, 720, 1167
DpnI GATC 6 cut(s) 204, 522, 558, 615, 932, 1200
DpnII GATC 6 cut(s) 202, 520, 556, 613, 930, 1198
DraIII CACNNNGTG 1 cut(s) 1027
EaeI YGGCCR 3 cut(s) 140, 830, 1219
EagI CGGCCG 1 cut(s) 140
Eam1104I CTCTTC 1 cut(s) 406
EarI CTCTTC 1 cut(s) 406
EclXI CGGCCG 1 cut(s) 140
Eco130I CCWWGG 4 cut(s) 587, 687, 1105, 1216
Eco24I GRGCYC 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 1110
Eco47I GGWCC 1 cut(s) 958
Eco52I CGGCCG 1 cut(s) 140
Eco81I CCTNAGG 1 cut(s) 1167
EcoO109I RGGNCCY 1 cut(s) 958
EcoRII CCWGG 2 cut(s) 314, 1119
EcoT14I CCWWGG 4 cut(s) 587, 687, 1105, 1216
EcoT38I GRGCYC 1 cut(s) 149
ErhI CCWWGG 4 cut(s) 587, 687, 1105, 1216
FaeI CATG 6 cut(s) 354, 691, 844, 867, 1021, 1109
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FaqI GGGAC 2 cut(s) 605, 633
FatI CATG 6 cut(s) 350, 687, 840, 863, 1017, 1105
FauNDI CATATG 1 cut(s) 644
FbaI TGATCA 2 cut(s) 202, 1198
FblI GTMKAC 1 cut(s) 602
Fnu4HI GCNGC 8 cut(s) 30, 140, 166, 395, 717, 907, 1141, 1263
FokI GGATG 3 cut(s) 89, 345, 1168
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 8 cut(s) 30, 140, 166, 395, 717, 907, 1141, 1263
FspBI CTAG 1 cut(s) 407
GluI GCNGC 8 cut(s) 30, 140, 166, 395, 717, 907, 1141, 1263
GsaI CCCAGC 1 cut(s) 172
HaeIII GGCC 5 cut(s) 56, 142, 832, 1221, 1262
HapII CCGG 3 cut(s) 143, 232, 1083
HgaI GACGC 1 cut(s) 98
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 6 cut(s) 354, 691, 844, 867, 1021, 1109
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HpaI GTTAAC 1 cut(s) 660
HpaII CCGG 3 cut(s) 143, 232, 1083
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 3 cut(s) 603, 660, 1236
Hpy188I TCNGA 5 cut(s) 113, 220, 325, 520, 699
Hpy188III TCNNGA 3 cut(s) 86, 187, 1166
Hpy8I GTNNAC 3 cut(s) 603, 660, 1236
HpyAV CCTTC 7 cut(s) 67, 92, 128, 470, 604, 661, 783
HpyCH4III ACNGT 3 cut(s) 329, 502, 596
HpyCH4IV ACGT 1 cut(s) 1254
HpyCH4V TGCA 6 cut(s) 102, 680, 704, 1021, 1043, 1140
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 716, 808, 1018
HpyF3I CTNAG 4 cut(s) 363, 417, 720, 1167
HpySE526I ACGT 1 cut(s) 1254
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 6 cut(s) 354, 691, 844, 867, 1021, 1109
Ksp22I TGATCA 2 cut(s) 202, 1198
KspAI GTTAAC 1 cut(s) 660
Kzo9I GATC 6 cut(s) 202, 520, 556, 613, 930, 1198
LmnI GCTCC 2 cut(s) 367, 1218
Lsp1109I GCAGC 4 cut(s) 177, 406, 703, 1152
LweI GCATC 2 cut(s) 111, 883
MabI ACCWGGT 1 cut(s) 1119
MaeI CTAG 1 cut(s) 407
MaeII ACGT 1 cut(s) 1254
MaeIII GTNAC 1 cut(s) 335
MalI GATC 6 cut(s) 204, 522, 558, 615, 932, 1200
MboI GATC 6 cut(s) 202, 520, 556, 613, 930, 1198
MboII GAAGA 5 cut(s) 209, 393, 468, 471, 1197
MfeI CAATTG 1 cut(s) 705
MflI RGATCY 2 cut(s) 520, 556
MhlI GDGCHC 3 cut(s) 149, 372, 1215
MlsI TGGCCA 1 cut(s) 832
MluCI AATT 5 cut(s) 43, 174, 254, 705, 1176
MluNI TGGCCA 1 cut(s) 832
Mox20I TGGCCA 1 cut(s) 832
MroXI GAANNNNTTC 1 cut(s) 1232
MscI TGGCCA 1 cut(s) 832
MseI TTAA 7 cut(s) 390, 659, 941, 983, 1049, 1089, 1179
MslI CAYNNNNRTG 2 cut(s) 342, 1154
Msp20I TGGCCA 1 cut(s) 832
MspA1I CMGCKG 1 cut(s) 168
MspCI CTTAAG 1 cut(s) 982
MspI CCGG 3 cut(s) 143, 232, 1083
MspR9I CCNGG 4 cut(s) 144, 316, 1084, 1121
MunI CAATTG 1 cut(s) 705
MvaI CCWGG 2 cut(s) 316, 1121
MwoI GCNNNNNNNGC 4 cut(s) 35, 716, 808, 1018
NciI CCSGG 2 cut(s) 144, 1084
NcoI CCATGG 2 cut(s) 687, 1105
NdeI CATATG 1 cut(s) 644
NdeII GATC 6 cut(s) 202, 520, 556, 613, 930, 1198
NlaIII CATG 6 cut(s) 354, 691, 844, 867, 1021, 1109
NlaIV GGNNCC 1 cut(s) 522
NmuCI GTSAC 1 cut(s) 335
OliI CACNNNNGTG 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 1232
PkrI GCNGC 8 cut(s) 31, 141, 167, 396, 718, 908, 1142, 1264
PpuMI RGGWCCY 1 cut(s) 958
PsiI TTATAA 2 cut(s) 540, 1073
Psp5II RGGWCCY 1 cut(s) 958
Psp6I CCWGG 2 cut(s) 314, 1119
PspFI CCCAGC 1 cut(s) 168
PspGI CCWGG 2 cut(s) 314, 1119
PspN4I GGNNCC 1 cut(s) 522
PspPI GGNCC 2 cut(s) 958, 1260
PspPPI RGGWCCY 1 cut(s) 958
PstNI CAGNNNCTG 2 cut(s) 19, 500
PsuI RGATCY 2 cut(s) 520, 556
PvuII CAGCTG 1 cut(s) 168
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
RseI CAYNNNNRTG 2 cut(s) 342, 1154
SaqAI TTAA 7 cut(s) 390, 659, 941, 983, 1049, 1089, 1179
SatI GCNGC 8 cut(s) 30, 140, 166, 395, 717, 907, 1141, 1263
Sau3AI GATC 6 cut(s) 202, 520, 556, 613, 930, 1198
Sau96I GGNCC 2 cut(s) 958, 1260
ScrFI CCNGG 4 cut(s) 144, 316, 1084, 1121
SduI GDGCHC 3 cut(s) 149, 372, 1215
SexAI ACCWGGT 1 cut(s) 1119
SfaNI GCATC 2 cut(s) 111, 883
SfcI CTRYAG 1 cut(s) 1247
SinI GGWCC 1 cut(s) 958
SmiMI CAYNNNNRTG 2 cut(s) 342, 1154
SmlI CTYRAG 1 cut(s) 982
SmoI CTYRAG 1 cut(s) 982
Sse9I AATT 5 cut(s) 43, 174, 254, 705, 1176
SsiI CCGC 4 cut(s) 29, 139, 907, 1263
SspMI CTAG 1 cut(s) 407
StyD4I CCNGG 4 cut(s) 142, 314, 1082, 1119
StyI CCWWGG 4 cut(s) 587, 687, 1105, 1216
TaaI ACNGT 3 cut(s) 329, 502, 596
TaiI ACGT 1 cut(s) 1257
TaqI TCGA 1 cut(s) 883
TasI AATT 5 cut(s) 43, 174, 254, 705, 1176
TatI WGTACW 1 cut(s) 430
TauI GCSGC 4 cut(s) 32, 142, 909, 1265
Tru1I TTAA 7 cut(s) 390, 659, 941, 983, 1049, 1089, 1179
Tru9I TTAA 7 cut(s) 390, 659, 941, 983, 1049, 1089, 1179
TscAI CASTG 2 cut(s) 20, 421
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 4 cut(s) 165, 394, 716, 1140
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 5 cut(s) 49, 210, 339, 794, 829
TspRI CASTG 2 cut(s) 20, 421
Vha464I CTTAAG 1 cut(s) 982
VpaK11BI GGWCC 1 cut(s) 958
XcmI CCANNNNNNNNNTGG 2 cut(s) 296, 559
XmiI GTMKAC 1 cut(s) 602
XmnI GAANNNNTTC 1 cut(s) 1232
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.