MD12G1013100.v1.1

Serine hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011279
Physical Location & Seq
Forward (+)
1402723 .. 1403832
1110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1013100.v1.1.491

Sequence Viewer

Length: 657 bp
ATGATTGAGAAATCTGTGATTTGTCCATTCCAGTTGAACTCCTTCCATTTGGTTGCACACTCCATGGGTTGTTTAATAGCAATTGCTTTAGCTGCTAAGCACCCCGAATTAGTAAAATCAGTCACTCTTGTAGCACCTCCTTACTTCCCTTCTAAAGATGGAGATAGCTTAAGAGTGCTTGAAAAACTTGCTGCAAGGAAACTATGGCCACCGTTCGTGTTTGGAACGTCATTTATGTCATGGTATGAACACCTGGGTCGATGCGTCTGTTTTCTTGTATGCCGACACCACAGGACTTGGGAGACGATCACAAAGCTACTTACCCGGAGAAGGGATCTTCATTTTATGGCTGTGGACTTCACTAGGCACACCCACCATTCAGCTTGGCACACCATGCACAATGTGATATGTGGGGGAGCAAAGTTAATGGAAGGTTACTTGCAAGTTTTGAGCAAAGCTGGGGTTAAAATTTGTGTTATCCATGGTGATAGAGACAATGTTGTTCCTATGGAGTGCATCAACAACATTCAGATGGCGGCTCCTGACGCAGAGGTTGACATTGTCAAAAATACTAATCACAATTCTGTGATCTTTGGTAGAGAGCGGGATTTTACTCGGCATGTAGAACATATTTGGGAATCGAACTCAGGACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0010224 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

219

Amino Acids

24.89

Weight (kDa)

8.82

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 12 - 198 6.8e-10 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 13 - 197 1.8e-07 Alpha/beta hydrolase family
Hydrolase_4 PF12146 16 - 193 1.2e-08 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17720 AT5G17780 AT5G17780
fragaria_vesca FvH4_2g19930 FvH4_2g19930 FvH4_2g19930
malus_domestica MD12G1013100.v1.1
prunus_persica Prupe.7G017500_v2.0.a1
pyrus_communis pycom12g01040
rosa_chinensis RchiOBHm_Chr3g0495891
rosa_laevigata RLG00000022582
rosa_multiflora Rmu_sc0012596.1_g000001
rosa_roxburghii Rroxscaffold_6G00389940
rosa_rugosa Rorug03G0276800
rosa_samantha Rh3AG324600 Rh3BG360600 Rh3BG360700 Rh3CG356800 Rh3DG359800
rosa_wichuraiana Rw3G028360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 604
AciI CCGC 2 cut(s) 536, 604
AclWI GGATC 1 cut(s) 342
AcoI YGGCCR 1 cut(s) 206
AcsI RAATTY 1 cut(s) 468
AdeI CACNNNGTG 1 cut(s) 403
AfiI CCNNNNNNNGG 2 cut(s) 330, 331
AflII CTTAAG 1 cut(s) 169
AgsI TTSAA 2 cut(s) 37, 182
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 5 cut(s) 92, 168, 316, 383, 458
AluI AGCT 5 cut(s) 92, 168, 316, 383, 458
Alw26I GTCTC 2 cut(s) 296, 486
AlwI GGATC 1 cut(s) 342
AoxI GGCC 1 cut(s) 206
ApeKI GCWGC 2 cut(s) 92, 191
ApoI RAATTY 1 cut(s) 468
Asp700I GAANNNNTTC 1 cut(s) 41
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 1 cut(s) 497
BalI TGGCCA 1 cut(s) 208
BbvI GCAGC 2 cut(s) 79, 178
BccI CCATC 2 cut(s) 152, 526
BciT130I CCWGG 1 cut(s) 254
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 296, 486
BfaI CTAG 1 cut(s) 363
BfrI CTTAAG 1 cut(s) 169
BisI GCNGC 3 cut(s) 93, 192, 537
BlpI GCTNAGC 1 cut(s) 96
BlsI GCNGC 3 cut(s) 94, 193, 538
Bme1390I CCNGG 2 cut(s) 254, 325
BmiI GGNNCC 1 cut(s) 540
BmrFI CCNGG 2 cut(s) 254, 325
BmsI GCATC 2 cut(s) 251, 525
Bpu1102I GCTNAGC 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 325
BsaJI CCNNGG 3 cut(s) 63, 253, 481
Bsc4I CCNNNNNNNGG 2 cut(s) 330, 331
Bse1I ACTGG 1 cut(s) 31
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 3 cut(s) 63, 253, 481
BseLI CCNNNNNNNGG 2 cut(s) 330, 331
BseNI ACTGG 1 cut(s) 31
BseXI GCAGC 2 cut(s) 79, 178
BseYI CCCAGC 1 cut(s) 458
BshFI GGCC 1 cut(s) 208
BsiSI CCGG 1 cut(s) 325
BslI CCNNNNNNNGG 2 cut(s) 330, 331
BsmAI GTCTC 2 cut(s) 296, 486
BsmBI CGTCTC 1 cut(s) 296
BsnI GGCC 1 cut(s) 208
Bsp143I GATC 3 cut(s) 306, 334, 588
Bsp1720I GCTNAGC 1 cut(s) 96
Bsp19I CCATGG 2 cut(s) 63, 481
BspACI CCGC 2 cut(s) 536, 604
BspANI GGCC 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 540
BspPI GGATC 1 cut(s) 342
BspTI CTTAAG 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 604
BsrI ACTGG 1 cut(s) 31
BssECI CCNNGG 3 cut(s) 63, 253, 481
BssMI GATC 3 cut(s) 306, 334, 588
BssT1I CCWWGG 2 cut(s) 63, 481
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 169
BstAPI GCANNNNNTGC 1 cut(s) 394
BstDEI CTNAG 2 cut(s) 96, 646
BstDSI CCRYGG 2 cut(s) 63, 481
BstKTI GATC 3 cut(s) 309, 337, 591
BstMAI GTCTC 2 cut(s) 296, 486
BstMBI GATC 3 cut(s) 306, 334, 588
BstMWI GCNNNNNNNGC 3 cut(s) 92, 394, 545
BstNI CCWGG 1 cut(s) 254
BstNSI RCATGY 1 cut(s) 623
BstSCI CCNGG 2 cut(s) 252, 323
BstV1I GCAGC 2 cut(s) 79, 178
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 1 cut(s) 208
BtgI CCRYGG 2 cut(s) 63, 481
CseI GACGC 2 cut(s) 253, 554
CspCI CAANNNNNGTGG 2 cut(s) 278, 313
CviAII CATG 5 cut(s) 64, 240, 394, 482, 620
CviJI RGCY 8 cut(s) 92, 168, 208, 316, 350, 383, 458, 539
CviKI_1 RGCY 8 cut(s) 92, 168, 208, 316, 350, 383, 458, 539
DdeI CTNAG 2 cut(s) 96, 646
DpnI GATC 3 cut(s) 308, 336, 590
DpnII GATC 3 cut(s) 306, 334, 588
DraIII CACNNNGTG 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 206
Eco130I CCWWGG 2 cut(s) 63, 481
EcoRII CCWGG 1 cut(s) 252
EcoT14I CCWWGG 2 cut(s) 63, 481
ErhI CCWWGG 2 cut(s) 63, 481
Esp3I CGTCTC 1 cut(s) 296
FaeI CATG 5 cut(s) 67, 243, 397, 485, 623
FatI CATG 5 cut(s) 63, 239, 393, 481, 619
FauI CCCGC 1 cut(s) 597
Fnu4HI GCNGC 3 cut(s) 93, 192, 537
Fsp4HI GCNGC 3 cut(s) 93, 192, 537
FspBI CTAG 1 cut(s) 363
GluI GCNGC 3 cut(s) 93, 192, 537
GsaI CCCAGC 1 cut(s) 462
HaeIII GGCC 1 cut(s) 208
HapII CCGG 1 cut(s) 325
HgaI GACGC 2 cut(s) 253, 554
Hin1II CATG 5 cut(s) 67, 243, 397, 485, 623
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 1 cut(s) 638
HpaII CCGG 1 cut(s) 325
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 2 cut(s) 355, 556
Hpy188I TCNGA 1 cut(s) 531
Hpy188III TCNNGA 2 cut(s) 542, 648
Hpy8I GTNNAC 2 cut(s) 355, 556
HpyAV CCTTC 4 cut(s) 52, 159, 324, 425
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4IV ACGT 2 cut(s) 227, 652
HpyCH4V TGCA 5 cut(s) 56, 194, 397, 442, 516
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 394, 545
HpyF3I CTNAG 2 cut(s) 96, 646
HpySE526I ACGT 2 cut(s) 227, 652
Hsp92II CATG 5 cut(s) 67, 243, 397, 485, 623
Kzo9I GATC 3 cut(s) 306, 334, 588
LmnI GCTCC 2 cut(s) 416, 544
LpnPI CCDG 8 cut(s) 44, 239, 266, 277, 338, 444, 555, 633
Lsp1109I GCAGC 2 cut(s) 79, 178
LweI GCATC 2 cut(s) 251, 525
MaeI CTAG 1 cut(s) 363
MaeII ACGT 2 cut(s) 227, 652
MaeIII GTNAC 2 cut(s) 121, 434
MalI GATC 3 cut(s) 308, 336, 590
MbiI CCGCTC 1 cut(s) 604
MboI GATC 3 cut(s) 306, 334, 588
MboII GAAGA 1 cut(s) 329
MfeI CAATTG 1 cut(s) 81
MflI RGATCY 1 cut(s) 334
MlsI TGGCCA 1 cut(s) 208
MluCI AATT 4 cut(s) 81, 107, 468, 580
MluNI TGGCCA 1 cut(s) 208
MnlI CCTC 2 cut(s) 147, 544
Mox20I TGGCCA 1 cut(s) 208
MroXI GAANNNNTTC 1 cut(s) 41
MscI TGGCCA 1 cut(s) 208
MseI TTAA 4 cut(s) 74, 170, 425, 465
MslI CAYNNNNRTG 1 cut(s) 530
Msp20I TGGCCA 1 cut(s) 208
MspCI CTTAAG 1 cut(s) 169
MspI CCGG 1 cut(s) 325
MspR9I CCNGG 2 cut(s) 254, 325
MunI CAATTG 1 cut(s) 81
MvaI CCWGG 1 cut(s) 254
MwoI GCNNNNNNNGC 3 cut(s) 92, 394, 545
NciI CCSGG 1 cut(s) 325
NcoI CCATGG 2 cut(s) 63, 481
NdeII GATC 3 cut(s) 306, 334, 588
NlaIII CATG 5 cut(s) 67, 243, 397, 485, 623
NlaIV GGNNCC 1 cut(s) 540
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 1 cut(s) 121
NspI RCATGY 1 cut(s) 623
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 1 cut(s) 638
PflFI GACNNNGTC 1 cut(s) 560
PkrI GCNGC 3 cut(s) 94, 193, 538
Psp6I CCWGG 1 cut(s) 252
PspFI CCCAGC 1 cut(s) 458
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 1 cut(s) 540
PsuI RGATCY 1 cut(s) 334
PsyI GACNNNGTC 1 cut(s) 560
RseI CAYNNNNRTG 1 cut(s) 530
SaqAI TTAA 4 cut(s) 74, 170, 425, 465
SatI GCNGC 3 cut(s) 93, 192, 537
Sau3AI GATC 3 cut(s) 306, 334, 588
ScrFI CCNGG 2 cut(s) 254, 325
SfaNI GCATC 2 cut(s) 251, 525
SmiMI CAYNNNNRTG 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse9I AATT 4 cut(s) 81, 107, 468, 580
SsiI CCGC 2 cut(s) 536, 604
SspMI CTAG 1 cut(s) 363
StyD4I CCNGG 2 cut(s) 252, 323
StyI CCWWGG 2 cut(s) 63, 481
TaaI ACNGT 1 cut(s) 213
TaiI ACGT 2 cut(s) 230, 655
TaqI TCGA 2 cut(s) 259, 641
TasI AATT 4 cut(s) 81, 107, 468, 580
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 1 cut(s) 638
Tru1I TTAA 4 cut(s) 74, 170, 425, 465
Tru9I TTAA 4 cut(s) 74, 170, 425, 465
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 2 cut(s) 92, 191
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 2 cut(s) 261, 329
Tth111I GACNNNGTC 1 cut(s) 560
Vha464I CTTAAG 1 cut(s) 169
XapI RAATTY 1 cut(s) 468
XceI RCATGY 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.