pycom12g01040

Serine hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011279
Physical Location & Seq
Forward (+)
923897 .. 926730
2834 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g01040.2

Sequence Viewer

Length: 1389 bp
ATGTCTTTTCTTCCACATCCAACTCAGCTCCCCATTTCTTCTCCACATGCCGCCTCAAATTTTCTCAGCAAAATGAGAAATCCGACGATAACCCTCGTATCTGCCTTGAACTTCATCGTCTTCCTCGTCTTTGACTTTCTCGACACCTTTCTCTGCGTCGTTTGCAGATACATTGACGAGTTGTTCGAAGGGCAGGCCTCCAGCTGTTACTGCACAGACAAGGAAGAACAGCAGGCGAGGAGGAATGGCGATGGACAAGAAGGTGAGGAGCTTTCGCTTTCAGAGACATTGTACGAGAGGAAAAACGTTTTTAGGAAAATGGGGTTTCTTGGATTTGTGAAAAAATGGAGGAAAAGTAAAAGTGAAAATCCAGGAAAGGTAAAAAACCTGAAAAATAGGTGGTCGGACTGTGGATGTCAGTCTTGTGTTTCATGGACCAATGATGCTGCCCAGAAGCTTCACTTTGTCATTGGGGAGCCTGAGACTTCTTCACAAGCCCTCACTGATGAGGATTGCAGAGGAAAGCCACCGGAGAATGTGATATTCCTGCACGGGTTTCTTTGTTCTTCCTCGTTTTGGACAGAAACCGTCTTCCCGAATCTTTCAGAACATGGGAAACGTAATTACAGATTGTTCGCCTTAGACCTGTTGGGATTTGGAAGAAGCCCAAAGCCAAGGGACTGTTTCTATACTTTGAAGGATCATTTGGAAATGATTGAGAAATCTGTGATTTGTCCATTCCAGTTGAACTCCTTCCATTTGGTTGCACACTCCATGGGTTGTTTAATAGCAATTGCTTTAGCTGCTAAGCACCCCGAATTAGTAAAATCGGTCACTCTTGTAGCACCTCCTTACTTCCCTTCCAAAGATGGAGATAGCTTAAGAGTACTTGAAAAAATAGCTGCAAGGAAACTATGGCCACCGTTCGTGTTTGGAATGTCATTAATGTCATGGTATGAACACCTGGGTCGATGCGTCTGTTTTCTTGTATGCCGACACCACAGGACTTGGGAGACGATCGCAAAGCTACTTACCCGGAGAAGGGATCTTCATTTTATGACTGTGGACTTCACTAGGCACACCCACCATTCAGCTTGGCACACCATGCACAATGTGATATGTGGGGGAGCAAAGTTAATGGAAGGTTACTTGCAAGTTTTGAGCAAAGCTGGGGTTAAAGTTTGTGTTATCCATGGTGATAGAGACAAAGTTGTTCCCATGGAGTGCAGCAATAACATTCAGATGGCGGCTCCTGACGCAGAGGTTAGCATTGTCAAAAATACTAATCACAATTCTGTGATCTTCGGTAGAAAGCGGGATTTTACTCGGCATGTAGAACATATTTGGGAATCGAACTCAGGACGTCGAATGTATGATAAATGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0010224 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

463

Amino Acids

52.8

Weight (kDa)

8.76

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17720 AT5G17780 AT5G17780
fragaria_vesca FvH4_2g19930 FvH4_2g19930 FvH4_2g19930
malus_domestica MD12G1013100.v1.1
prunus_persica Prupe.7G017500_v2.0.a1
pyrus_communis pycom12g01040
rosa_chinensis RchiOBHm_Chr3g0495891
rosa_laevigata RLG00000022582
rosa_multiflora Rmu_sc0012596.1_g000001
rosa_roxburghii Rroxscaffold_6G00389940
rosa_rugosa Rorug03G0276800
rosa_samantha Rh3AG324600 Rh3BG360600 Rh3BG360700 Rh3CG356800 Rh3DG359800
rosa_wichuraiana Rw3G028360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1366
AciI CCGC 3 cut(s) 51, 1247, 1315
AclI AACGTT 1 cut(s) 306
AclWI GGATC 2 cut(s) 708, 1053
AcoI YGGCCR 1 cut(s) 917
AcsI RAATTY 1 cut(s) 58
AcyI GRCGYC 1 cut(s) 1363
AdeI CACNNNGTG 1 cut(s) 1114
AfaI GTAC 2 cut(s) 293, 888
AfiI CCNNNNNNNGG 3 cut(s) 576, 1041, 1042
AflII CTTAAG 1 cut(s) 880
AgsI TTSAA 4 cut(s) 109, 697, 748, 893
AjnI CCWGG 2 cut(s) 370, 963
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
Alw26I GTCTC 4 cut(s) 278, 476, 1007, 1197
AlwI GGATC 2 cut(s) 708, 1053
AoxI GGCC 2 cut(s) 195, 917
ApeKI GCWGC 4 cut(s) 446, 803, 902, 1227
ApoI RAATTY 1 cut(s) 58
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AseI ATTAAT 1 cut(s) 944
Asp700I GAANNNNTTC 1 cut(s) 752
AspS9I GGNCC 1 cut(s) 435
AsuC2I CCSGG 1 cut(s) 1036
AsuHPI GGTGA 2 cut(s) 275, 1208
AsuII TTCGAA 1 cut(s) 186
AvaII GGWCC 1 cut(s) 435
BaeI ACNNNNGTAYC 2 cut(s) 81, 114
BalI TGGCCA 1 cut(s) 919
BbsI GAAGAC 2 cut(s) 112, 583
BbvI GCAGC 4 cut(s) 433, 790, 889, 1239
BccI CCATC 3 cut(s) 245, 863, 1237
BciT130I CCWGG 2 cut(s) 372, 965
BcnI CCSGG 1 cut(s) 1036
BcoDI GTCTC 4 cut(s) 278, 476, 1007, 1197
BfaI CTAG 1 cut(s) 1074
BfrI CTTAAG 1 cut(s) 880
BisI GCNGC 6 cut(s) 51, 447, 804, 903, 1228, 1248
BlpI GCTNAGC 1 cut(s) 807
BlsI GCNGC 6 cut(s) 52, 448, 805, 904, 1229, 1249
BmcAI AGTACT 1 cut(s) 888
Bme1390I CCNGG 3 cut(s) 372, 965, 1036
Bme18I GGWCC 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 2 cut(s) 477, 1251
BmrFI CCNGG 3 cut(s) 372, 965, 1036
BmsI GCATC 2 cut(s) 433, 962
BpiI GAAGAC 2 cut(s) 112, 583
BpmI CTGGAG 1 cut(s) 184
Bpu1102I GCTNAGC 1 cut(s) 807
Bpu14I TTCGAA 1 cut(s) 186
BpuMI CCSGG 1 cut(s) 1036
BsaHI GRCGYC 1 cut(s) 1363
BsaJI CCNNGG 5 cut(s) 674, 774, 964, 1192, 1218
BsaWI WCCGGW 1 cut(s) 529
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bsc4I CCNNNNNNNGG 3 cut(s) 576, 1041, 1042
Bse1I ACTGG 1 cut(s) 742
BseBI CCWGG 2 cut(s) 372, 965
BseDI CCNNGG 5 cut(s) 674, 774, 964, 1192, 1218
BseGI GGATG 2 cut(s) 16, 419
BseLI CCNNNNNNNGG 3 cut(s) 576, 1041, 1042
BseMII CTCAG 4 cut(s) 38, 79, 471, 1371
BseNI ACTGG 1 cut(s) 742
BseRI GAGGAG 2 cut(s) 253, 281
BseXI GCAGC 4 cut(s) 433, 790, 889, 1239
BseYI CCCAGC 1 cut(s) 1169
BsgI GTGCAG 3 cut(s) 196, 533, 1246
Bsh1285I CGRYCG 1 cut(s) 1020
BshFI GGCC 2 cut(s) 197, 919
BsiEI CGRYCG 1 cut(s) 1020
BsiSI CCGG 2 cut(s) 530, 1036
BslFI GGGAC 1 cut(s) 692
BslI CCNNNNNNNGG 3 cut(s) 576, 1041, 1042
BsmAI GTCTC 4 cut(s) 278, 476, 1007, 1197
BsmBI CGTCTC 1 cut(s) 1007
BsmFI GGGAC 1 cut(s) 692
BsnI GGCC 2 cut(s) 197, 919
Bsp119I TTCGAA 1 cut(s) 186
Bsp143I GATC 4 cut(s) 700, 1017, 1045, 1299
Bsp1720I GCTNAGC 1 cut(s) 807
Bsp19I CCATGG 3 cut(s) 774, 1192, 1218
BspACI CCGC 3 cut(s) 51, 1247, 1315
BspANI GGCC 2 cut(s) 197, 919
BspCNI CTCAG 4 cut(s) 37, 78, 472, 1370
BspLI GGNNCC 2 cut(s) 477, 1251
BspPI GGATC 2 cut(s) 708, 1053
BspT104I TTCGAA 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 880
BsrI ACTGG 1 cut(s) 742
BssECI CCNNGG 5 cut(s) 674, 774, 964, 1192, 1218
BssMI GATC 4 cut(s) 700, 1017, 1045, 1299
BssNI GRCGYC 1 cut(s) 1363
BssT1I CCWWGG 4 cut(s) 674, 774, 1192, 1218
Bst2UI CCWGG 2 cut(s) 372, 965
Bst4CI ACNGT 5 cut(s) 410, 589, 683, 924, 1063
BstACI GRCGYC 1 cut(s) 1363
BstAFI CTTAAG 1 cut(s) 880
BstAPI GCANNNNNTGC 1 cut(s) 1105
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 195, 234
BstDEI CTNAG 6 cut(s) 24, 65, 480, 640, 807, 1357
BstDSI CCRYGG 3 cut(s) 774, 1192, 1218
BstF5I GGATG 2 cut(s) 16, 419
BstKTI GATC 4 cut(s) 703, 1020, 1048, 1302
BstMAI GTCTC 4 cut(s) 278, 476, 1007, 1197
BstMBI GATC 4 cut(s) 700, 1017, 1045, 1299
BstMCI CGRYCG 1 cut(s) 1020
BstMWI GCNNNNNNNGC 5 cut(s) 162, 210, 803, 1105, 1256
BstNI CCWGG 2 cut(s) 372, 965
BstNSI RCATGY 2 cut(s) 50, 1334
BstSCI CCNGG 3 cut(s) 370, 963, 1034
BstV1I GCAGC 4 cut(s) 433, 790, 889, 1239
BstV2I GAAGAC 2 cut(s) 112, 583
BstX2I RGATCY 1 cut(s) 1045
BstYI RGATCY 1 cut(s) 1045
BsuRI GGCC 2 cut(s) 197, 919
BtgI CCRYGG 3 cut(s) 774, 1192, 1218
BtgZI GCGATG 1 cut(s) 264
BtsCI GGATG 2 cut(s) 16, 419
BtsIMutI CAGTG 1 cut(s) 501
Cac8I GCNNGC 2 cut(s) 195, 234
Cfr13I GGNCC 1 cut(s) 435
CseI GACGC 3 cut(s) 145, 964, 1265
Csp6I GTAC 2 cut(s) 292, 887
CspCI CAANNNNNGTGG 2 cut(s) 989, 1024
CviAII CATG 9 cut(s) 47, 432, 611, 775, 951, 1105, 1193, 1219, 1331
CviQI GTAC 2 cut(s) 292, 887
DdeI CTNAG 6 cut(s) 24, 65, 480, 640, 807, 1357
DpnI GATC 4 cut(s) 702, 1019, 1047, 1301
DpnII GATC 4 cut(s) 700, 1017, 1045, 1299
DraIII CACNNNGTG 1 cut(s) 1114
EaeI YGGCCR 1 cut(s) 917
Eco130I CCWWGG 4 cut(s) 674, 774, 1192, 1218
Eco147I AGGCCT 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 435
EcoRII CCWGG 2 cut(s) 370, 963
EcoT14I CCWWGG 4 cut(s) 674, 774, 1192, 1218
ErhI CCWWGG 4 cut(s) 674, 774, 1192, 1218
Esp3I CGTCTC 1 cut(s) 1007
FaeI CATG 9 cut(s) 50, 435, 614, 778, 954, 1108, 1196, 1222, 1334
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 1 cut(s) 692
FatI CATG 9 cut(s) 46, 431, 610, 774, 950, 1104, 1192, 1218, 1330
FauI CCCGC 1 cut(s) 1308
Fnu4HI GCNGC 6 cut(s) 51, 447, 804, 903, 1228, 1248
FokI GGATG 2 cut(s) 3, 426
Fsp4HI GCNGC 6 cut(s) 51, 447, 804, 903, 1228, 1248
FspBI CTAG 1 cut(s) 1074
GluI GCNGC 6 cut(s) 51, 447, 804, 903, 1228, 1248
GsaI CCCAGC 1 cut(s) 1173
GsuI CTGGAG 1 cut(s) 184
HaeIII GGCC 2 cut(s) 197, 919
HapII CCGG 2 cut(s) 530, 1036
HgaI GACGC 3 cut(s) 145, 964, 1265
Hin1I GRCGYC 1 cut(s) 1363
Hin1II CATG 9 cut(s) 50, 435, 614, 778, 954, 1108, 1196, 1222, 1334
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 2 cut(s) 598, 1349
HpaII CCGG 2 cut(s) 530, 1036
HphI GGTGA 2 cut(s) 275, 1208
Hpy166II GTNNAC 1 cut(s) 1066
Hpy188I TCNGA 5 cut(s) 84, 283, 406, 607, 1242
Hpy188III TCNNGA 4 cut(s) 140, 595, 1253, 1359
Hpy8I GTNNAC 1 cut(s) 1066
Hpy99I CGWCG 3 cut(s) 88, 161, 1368
HpyAV CCTTC 7 cut(s) 182, 254, 691, 763, 870, 1035, 1136
HpyCH4III ACNGT 5 cut(s) 410, 589, 683, 924, 1063
HpyCH4IV ACGT 3 cut(s) 306, 619, 1363
HpyCH4V TGCA 9 cut(s) 165, 213, 516, 550, 767, 905, 1108, 1153, 1227
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 210, 803, 1105, 1256
HpyF3I CTNAG 6 cut(s) 24, 65, 480, 640, 807, 1357
HpySE526I ACGT 3 cut(s) 306, 619, 1363
Hsp92I GRCGYC 1 cut(s) 1363
Hsp92II CATG 9 cut(s) 50, 435, 614, 778, 954, 1108, 1196, 1222, 1334
Kzo9I GATC 4 cut(s) 700, 1017, 1045, 1299
LmnI GCTCC 5 cut(s) 33, 268, 475, 1127, 1255
Lsp1109I GCAGC 4 cut(s) 433, 790, 889, 1239
LweI GCATC 2 cut(s) 433, 962
MaeI CTAG 1 cut(s) 1074
MaeII ACGT 3 cut(s) 306, 619, 1363
MaeIII GTNAC 3 cut(s) 206, 832, 1145
MalI GATC 4 cut(s) 702, 1019, 1047, 1301
MboI GATC 4 cut(s) 700, 1017, 1045, 1299
MboII GAAGA 9 cut(s) 30, 112, 236, 480, 558, 583, 672, 1040, 1294
MfeI CAATTG 1 cut(s) 792
MflI RGATCY 1 cut(s) 1045
MlsI TGGCCA 1 cut(s) 919
MluCI AATT 5 cut(s) 58, 622, 792, 818, 1291
MluNI TGGCCA 1 cut(s) 919
MmeI TCCRAC 3 cut(s) 44, 107, 384
Mox20I TGGCCA 1 cut(s) 919
MroXI GAANNNNTTC 1 cut(s) 752
MscI TGGCCA 1 cut(s) 919
MseI TTAA 6 cut(s) 785, 881, 944, 1136, 1176, 1387
MslI CAYNNNNRTG 1 cut(s) 1241
Msp20I TGGCCA 1 cut(s) 919
MspA1I CMGCKG 1 cut(s) 204
MspCI CTTAAG 1 cut(s) 880
MspI CCGG 2 cut(s) 530, 1036
MspR9I CCNGG 3 cut(s) 372, 965, 1036
MunI CAATTG 1 cut(s) 792
MvaI CCWGG 2 cut(s) 372, 965
MwoI GCNNNNNNNGC 5 cut(s) 162, 210, 803, 1105, 1256
NciI CCSGG 1 cut(s) 1036
NcoI CCATGG 3 cut(s) 774, 1192, 1218
NdeII GATC 4 cut(s) 700, 1017, 1045, 1299
NlaIII CATG 9 cut(s) 50, 435, 614, 778, 954, 1108, 1196, 1222, 1334
NlaIV GGNNCC 2 cut(s) 477, 1251
NmeAIII GCCGAG 1 cut(s) 1306
NmuCI GTSAC 1 cut(s) 832
NspI RCATGY 2 cut(s) 50, 1334
NspV TTCGAA 1 cut(s) 186
PceI AGGCCT 1 cut(s) 197
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 183
PdmI GAANNNNTTC 1 cut(s) 752
PfeI GAWTC 2 cut(s) 598, 1349
PfoI TCCNGGA 1 cut(s) 370
PkrI GCNGC 6 cut(s) 52, 448, 805, 904, 1229, 1249
Ple19I CGATCG 1 cut(s) 1020
PshBI ATTAAT 1 cut(s) 944
Psp1406I AACGTT 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 370, 963
PspFI CCCAGC 1 cut(s) 1169
PspGI CCWGG 2 cut(s) 370, 963
PspN4I GGNNCC 2 cut(s) 477, 1251
PspPI GGNCC 1 cut(s) 435
PsuI RGATCY 1 cut(s) 1045
PvuI CGATCG 1 cut(s) 1020
PvuII CAGCTG 1 cut(s) 204
RsaI GTAC 2 cut(s) 293, 888
RsaNI GTAC 2 cut(s) 292, 887
RseI CAYNNNNRTG 1 cut(s) 1241
SaqAI TTAA 6 cut(s) 785, 881, 944, 1136, 1176, 1387
SatI GCNGC 6 cut(s) 51, 447, 804, 903, 1228, 1248
Sau3AI GATC 4 cut(s) 700, 1017, 1045, 1299
Sau96I GGNCC 1 cut(s) 435
ScaI AGTACT 1 cut(s) 888
ScrFI CCNGG 3 cut(s) 372, 965, 1036
SfaNI GCATC 2 cut(s) 433, 962
SfuI TTCGAA 1 cut(s) 186
SinI GGWCC 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 1241
SmlI CTYRAG 1 cut(s) 880
SmoI CTYRAG 1 cut(s) 880
Sse9I AATT 5 cut(s) 58, 622, 792, 818, 1291
SseBI AGGCCT 1 cut(s) 197
SsiI CCGC 3 cut(s) 51, 1247, 1315
SspMI CTAG 1 cut(s) 1074
StuI AGGCCT 1 cut(s) 197
StyD4I CCNGG 3 cut(s) 370, 963, 1034
StyI CCWWGG 4 cut(s) 674, 774, 1192, 1218
TaaI ACNGT 5 cut(s) 410, 589, 683, 924, 1063
TaiI ACGT 3 cut(s) 309, 622, 1366
TaqI TCGA 5 cut(s) 141, 186, 970, 1352, 1366
TaqII GACCGA 1 cut(s) 820
TasI AATT 5 cut(s) 58, 622, 792, 818, 1291
TatI WGTACW 1 cut(s) 886
TauI GCSGC 2 cut(s) 53, 1250
TfiI GAWTC 2 cut(s) 598, 1349
Tru1I TTAA 6 cut(s) 785, 881, 944, 1136, 1176, 1387
Tru9I TTAA 6 cut(s) 785, 881, 944, 1136, 1176, 1387
TscAI CASTG 1 cut(s) 508
TseFI GTSAC 1 cut(s) 832
TseI GCWGC 4 cut(s) 446, 803, 902, 1227
Tsp45I GTSAC 1 cut(s) 832
TspDTI ATGAA 4 cut(s) 103, 420, 972, 1040
TspRI CASTG 1 cut(s) 508
Vha464I CTTAAG 1 cut(s) 880
VpaK11BI GGWCC 1 cut(s) 435
VspI ATTAAT 1 cut(s) 944
XapI RAATTY 1 cut(s) 58
XceI RCATGY 2 cut(s) 50, 1334
XmnI GAANNNNTTC 1 cut(s) 752
XspI CTAG 1 cut(s) 1074
ZraI GACGTC 1 cut(s) 1364
ZrmI AGTACT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.