RLG00000022582

Serine hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011279
Physical Location & Seq
Forward (+)
4540589 .. 4542703
2115 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022582

Sequence Viewer

Length: 1269 bp
ATGTTTCCTCGGTGGCTGAAAAAGCCGGCAACAGCTCTCAATTCTGCCTTGGGCTTCATTGTGTTCCTTATATTTGACTTTCTTGATGCCATTTTGTGTGTCCTCTTCAGATATATTGATCACTTGTTTGAAGGGCGGCCGGGTTCTTGTTACTGTGGACGCAAGGAAATAGAGCTGGTCAGGAATGTGGTTGGTGATGAAGAAGATCACCAGCTTTCAGAGACTTTATACCGGAGGAAAAACGTTTTCAGGGAAATGGGTCTGGTTGGATTTGCAAAAAGCTGCCAGAACACAAGTGCTGGCGGAGTTGCCAGGTGGTCTGACTGTGGATGTCACTTTTGTGTTTCATGGCTGAAGGATACTCATCACAACCTACATCTTGTTATTATGCAGCCTTCATCACCAGCCATCACTGAGGATTGCAGAGTACAAGCAGCCGAGAATGTGATATTCCTTCACGGGTTTCTTTCTTCTTCCTCGTTTTGGGCAGAAACAGTGTTTCCAAACATTTCAGAATCCGTGAGTCGGCAATATAGGTTGTTTGCCATAGACCTATTGGGATTTGGTTTAAGCCCAAAGCCAAGGGACTGTTTCTATACTTTGAAGGATCACATAGAAATGATTGAGAAATCTGTCATCTGCCCCTTCCAGTTGACTTCTTTCCATTTGGTTGCACACTCCATGGGTTGTCTGATTGCAATCGCTTTAGCTGCTAAGCACTCGGAATTAGTAAAATCAATCACTCTTGTAGCACCTCCTTACTTCCCTTCTAAAGATGGAGATAGTTTAAGAGTGCTTCACAAGCTTGCTGAAAGGAAACTGTGGCCACTTACCTTGTTTGGATCATCATTTATGTCATGGTATGAGCATCTGGGTCGATGTGTCTGTTTCCTTCTATGCCGCAACCACAGGACATGGGAGAGGATTGTAAAGTTATTCACTTGGAGAAGGGATCTTCATTTTATGACAATGGACTTGACTAGGCACACCCATCACTCAGCTTGGCACACCATGCACAATGTGATATGTGGGGGTGCAAAGTTAATGGAATGCTACTTGGAAGTTATAGGCAAAGCTGGGGTTGAAGTGTTTGTGATCCATGGAGATAGAGACCAGGTTGTCCCTGTAGAGTGCAGCAACAACATTCAGATGACAGTTCCTGAGGCACAAGTTAACATCATTAAAAATGCTGATCATTGCTCTGTACTCCTTGGCCGAGAGAAAGACTTCACCCTCCATCTAGAGCACGTTTGGGCCGCTTCCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0010224 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

423

Amino Acids

48.16

Weight (kDa)

7.32

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 147 - 399 9.9e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 149 - 403 2.4e-27 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 149 - 402 5.9e-18 Alpha/beta hydrolase family
LIDHydrolase PF10230 169 - 399 4.7e-06 Lipid-droplet associated hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17720 AT5G17780 AT5G17780
fragaria_vesca FvH4_2g19930 FvH4_2g19930 FvH4_2g19930
malus_domestica MD12G1013100.v1.1
prunus_persica Prupe.7G017500_v2.0.a1
pyrus_communis pycom12g01040
rosa_chinensis RchiOBHm_Chr3g0495891
rosa_laevigata RLG00000022582
rosa_multiflora Rmu_sc0012596.1_g000001
rosa_roxburghii Rroxscaffold_6G00389940
rosa_rugosa Rorug03G0276800
rosa_samantha Rh3AG324600 Rh3BG360600 Rh3BG360700 Rh3CG356800 Rh3DG359800
rosa_wichuraiana Rw3G028360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1118
AciI CCGC 4 cut(s) 136, 303, 901, 1257
AclI AACGTT 1 cut(s) 243
AclWI GGATC 4 cut(s) 615, 850, 960, 1090
AcoI YGGCCR 3 cut(s) 137, 824, 1213
AcuI CTGAAG 2 cut(s) 91, 374
AdeI CACNNNGTG 1 cut(s) 1021
AfaI GTAC 2 cut(s) 429, 1206
AfiI CCNNNNNNNGG 2 cut(s) 483, 525
AgsI TTSAA 3 cut(s) 131, 604, 1085
AjnI CCWGG 2 cut(s) 311, 1113
AleI CACNNNNGTG 1 cut(s) 339
AluBI AGCT 8 cut(s) 35, 175, 214, 282, 710, 805, 1001, 1076
AluI AGCT 8 cut(s) 35, 175, 214, 282, 710, 805, 1001, 1076
Alw21I GWGCWC 1 cut(s) 1248
Alw26I GTCTC 2 cut(s) 215, 1104
AlwI GGATC 4 cut(s) 615, 850, 960, 1090
AlwNI CAGNNNCTG 1 cut(s) 1160
AoxI GGCC 4 cut(s) 137, 824, 1213, 1254
ApeKI GCWGC 5 cut(s) 282, 391, 434, 710, 1134
Asp700I GAANNNNTTC 1 cut(s) 1226
AspS9I GGNCC 1 cut(s) 1254
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 4 cut(s) 200, 206, 393, 1222
AxyI CCTNAGG 1 cut(s) 1161
BalI TGGCCA 1 cut(s) 826
Bbv12I GWGCWC 1 cut(s) 1248
BbvI GCAGC 5 cut(s) 269, 403, 446, 697, 1146
BccI CCATC 4 cut(s) 416, 770, 999, 1245
BcgI CGANNNNNNTGC 2 cut(s) 857, 891
BciT130I CCWGG 2 cut(s) 313, 1115
BciVI GTATCC 1 cut(s) 352
BclI TGATCA 2 cut(s) 118, 1192
BcnI CCSGG 1 cut(s) 141
BcoDI GTCTC 2 cut(s) 215, 1104
BfaI CTAG 2 cut(s) 981, 1241
BfmI CTRYAG 1 cut(s) 1125
BfuI GTATCC 1 cut(s) 352
BisI GCNGC 8 cut(s) 137, 283, 392, 435, 711, 901, 1135, 1257
BlpI GCTNAGC 1 cut(s) 714
BlsI GCNGC 8 cut(s) 138, 284, 393, 436, 712, 902, 1136, 1258
Bme1390I CCNGG 3 cut(s) 141, 313, 1115
BmgT120I GGNCC 1 cut(s) 1254
BmrFI CCNGG 3 cut(s) 141, 313, 1115
BmsI GCATC 2 cut(s) 76, 877
Bpu1102I GCTNAGC 1 cut(s) 714
BpuMI CCSGG 1 cut(s) 141
BsaBI GATNNNNATC 2 cut(s) 363, 698
BsaI GGTCTC 1 cut(s) 1104
BsaJI CCNNGG 6 cut(s) 8, 48, 581, 681, 1099, 1210
BsaWI WCCGGW 1 cut(s) 231
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 525
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 649
Bse21I CCTNAGG 1 cut(s) 1161
Bse3DI GCAATG 1 cut(s) 1195
Bse8I GATNNNNATC 2 cut(s) 363, 698
BseBI CCWGG 2 cut(s) 313, 1115
BseDI CCNNGG 6 cut(s) 8, 48, 581, 681, 1099, 1210
BseGI GGATG 1 cut(s) 335
BseJI GATNNNNATC 2 cut(s) 363, 698
BseLI CCNNNNNNNGG 2 cut(s) 483, 525
BseMI GCAATG 1 cut(s) 1195
BseMII CTCAG 3 cut(s) 405, 1011, 1152
BseNI ACTGG 1 cut(s) 649
BseX3I CGGCCG 1 cut(s) 137
BseXI GCAGC 5 cut(s) 269, 403, 446, 697, 1146
BseYI CCCAGC 1 cut(s) 1076
BsgI GTGCAG 1 cut(s) 1153
Bsh1285I CGRYCG 1 cut(s) 140
BshFI GGCC 4 cut(s) 139, 826, 1215, 1256
BsiEI CGRYCG 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 1248
BsiSI CCGG 3 cut(s) 26, 140, 232
BslFI GGGAC 2 cut(s) 599, 1106
BslI CCNNNNNNNGG 2 cut(s) 483, 525
BsmAI GTCTC 2 cut(s) 215, 1104
BsmFI GGGAC 2 cut(s) 599, 1106
BsmI GAATGC 1 cut(s) 1055
BsnI GGCC 4 cut(s) 139, 826, 1215, 1256
Bso31I GGTCTC 1 cut(s) 1104
Bsp1286I GDGCHC 1 cut(s) 1248
Bsp143I GATC 7 cut(s) 118, 205, 607, 842, 952, 1095, 1192
Bsp1720I GCTNAGC 1 cut(s) 714
Bsp19I CCATGG 2 cut(s) 681, 1099
BspACI CCGC 4 cut(s) 136, 303, 901, 1257
BspANI GGCC 4 cut(s) 139, 826, 1215, 1256
BspCNI CTCAG 3 cut(s) 406, 1010, 1153
BspPI GGATC 4 cut(s) 615, 850, 960, 1090
BspTNI GGTCTC 1 cut(s) 1104
BsrDI GCAATG 1 cut(s) 1195
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 649
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 6 cut(s) 8, 48, 581, 681, 1099, 1210
BssMI GATC 7 cut(s) 118, 205, 607, 842, 952, 1095, 1192
BssT1I CCWWGG 5 cut(s) 48, 581, 681, 1099, 1210
Bst2UI CCWGG 2 cut(s) 313, 1115
Bst4CI ACNGT 6 cut(s) 155, 326, 496, 590, 822, 1156
Bst6I CTCTTC 1 cut(s) 110
BstAPI GCANNNNNTGC 1 cut(s) 1012
BstC8I GCNNGC 3 cut(s) 27, 301, 807
BstDEI CTNAG 4 cut(s) 414, 714, 997, 1161
BstDSI CCRYGG 2 cut(s) 681, 1099
BstF5I GGATG 1 cut(s) 335
BstKTI GATC 7 cut(s) 121, 208, 610, 845, 955, 1098, 1195
BstMAI GTCTC 2 cut(s) 215, 1104
BstMBI GATC 7 cut(s) 118, 205, 607, 842, 952, 1095, 1192
BstMCI CGRYCG 1 cut(s) 140
BstMWI GCNNNNNNNGC 4 cut(s) 22, 710, 802, 1012
BstNI CCWGG 2 cut(s) 313, 1115
BstSCI CCNGG 3 cut(s) 139, 311, 1113
BstSFI CTRYAG 1 cut(s) 1125
BstV1I GCAGC 5 cut(s) 269, 403, 446, 697, 1146
BstX2I RGATCY 1 cut(s) 952
BstYI RGATCY 1 cut(s) 952
BstZI CGGCCG 1 cut(s) 137
Bsu36I CCTNAGG 1 cut(s) 1161
BsuI GTATCC 1 cut(s) 352
BsuRI GGCC 4 cut(s) 139, 826, 1215, 1256
BtgI CCRYGG 2 cut(s) 681, 1099
BtsCI GGATG 1 cut(s) 335
BtsIMutI CAGTG 2 cut(s) 411, 501
Cac8I GCNNGC 3 cut(s) 27, 301, 807
CaiI CAGNNNCTG 1 cut(s) 1160
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 1254
CseI GACGC 1 cut(s) 168
CsiI ACCWGGT 1 cut(s) 1113
Csp6I GTAC 2 cut(s) 428, 1205
CspCI CAANNNNNGTGG 2 cut(s) 816, 851
CviAII CATG 6 cut(s) 348, 682, 858, 915, 1012, 1100
CviQI GTAC 2 cut(s) 428, 1205
DdeI CTNAG 4 cut(s) 414, 714, 997, 1161
DpnI GATC 7 cut(s) 120, 207, 609, 844, 954, 1097, 1194
DpnII GATC 7 cut(s) 118, 205, 607, 842, 952, 1095, 1192
DraIII CACNNNGTG 1 cut(s) 1021
DrdI GACNNNNNNGTC 1 cut(s) 1118
DseDI GACNNNNNNGTC 1 cut(s) 1118
EaeI YGGCCR 3 cut(s) 137, 824, 1213
EagI CGGCCG 1 cut(s) 137
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 1 cut(s) 318
EclXI CGGCCG 1 cut(s) 137
Eco130I CCWWGG 5 cut(s) 48, 581, 681, 1099, 1210
Eco31I GGTCTC 1 cut(s) 1104
Eco52I CGGCCG 1 cut(s) 137
Eco57I CTGAAG 2 cut(s) 91, 374
Eco81I CCTNAGG 1 cut(s) 1161
EcoRII CCWGG 2 cut(s) 311, 1113
EcoT14I CCWWGG 5 cut(s) 48, 581, 681, 1099, 1210
ErhI CCWWGG 5 cut(s) 48, 581, 681, 1099, 1210
FaeI CATG 6 cut(s) 351, 685, 861, 918, 1015, 1103
FaqI GGGAC 2 cut(s) 599, 1106
FatI CATG 6 cut(s) 347, 681, 857, 914, 1011, 1099
FbaI TGATCA 2 cut(s) 118, 1192
Fnu4HI GCNGC 8 cut(s) 137, 283, 392, 435, 711, 901, 1135, 1257
FokI GGATG 1 cut(s) 342
Fsp4HI GCNGC 8 cut(s) 137, 283, 392, 435, 711, 901, 1135, 1257
FspBI CTAG 2 cut(s) 981, 1241
GluI GCNGC 8 cut(s) 137, 283, 392, 435, 711, 901, 1135, 1257
GsaI CCCAGC 1 cut(s) 1080
HaeIII GGCC 4 cut(s) 139, 826, 1215, 1256
HapII CCGG 3 cut(s) 26, 140, 232
HgaI GACGC 1 cut(s) 168
Hin1II CATG 6 cut(s) 351, 685, 861, 918, 1015, 1103
HincII GTYRAC 2 cut(s) 654, 1174
HindII GTYRAC 2 cut(s) 654, 1174
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 2 cut(s) 515, 523
HpaI GTTAAC 1 cut(s) 1174
HpaII CCGG 3 cut(s) 26, 140, 232
HphI GGTGA 4 cut(s) 200, 206, 393, 1222
Hpy166II GTNNAC 3 cut(s) 158, 654, 1174
Hpy188I TCNGA 7 cut(s) 110, 220, 322, 514, 693, 724, 1149
Hpy188III TCNNGA 4 cut(s) 83, 181, 1160, 1241
Hpy8I GTNNAC 3 cut(s) 158, 654, 1174
HpyAV CCTTC 9 cut(s) 125, 349, 405, 464, 598, 655, 777, 902, 942
HpyCH4III ACNGT 6 cut(s) 155, 326, 496, 590, 822, 1156
HpyCH4IV ACGT 2 cut(s) 243, 1248
HpyCH4V TGCA 8 cut(s) 275, 391, 423, 674, 698, 1015, 1037, 1134
HpyF10VI GCNNNNNNNGC 4 cut(s) 22, 710, 802, 1012
HpyF3I CTNAG 4 cut(s) 414, 714, 997, 1161
HpySE526I ACGT 2 cut(s) 243, 1248
Hsp92II CATG 6 cut(s) 351, 685, 861, 918, 1015, 1103
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Ksp22I TGATCA 2 cut(s) 118, 1192
KspAI GTTAAC 1 cut(s) 1174
Kzo9I GATC 7 cut(s) 118, 205, 607, 842, 952, 1095, 1192
Lsp1109I GCAGC 5 cut(s) 269, 403, 446, 697, 1146
LweI GCATC 2 cut(s) 76, 877
MabI ACCWGGT 1 cut(s) 1113
MaeI CTAG 2 cut(s) 981, 1241
MaeII ACGT 2 cut(s) 243, 1248
MaeIII GTNAC 2 cut(s) 149, 332
MalI GATC 7 cut(s) 120, 207, 609, 844, 954, 1097, 1194
MboI GATC 7 cut(s) 118, 205, 607, 842, 952, 1095, 1192
MboII GAAGA 6 cut(s) 97, 212, 215, 462, 465, 947
MflI RGATCY 1 cut(s) 952
MhlI GDGCHC 1 cut(s) 1248
MlsI TGGCCA 1 cut(s) 826
MluCI AATT 2 cut(s) 40, 725
MluNI TGGCCA 1 cut(s) 826
MlyI GAGTC 1 cut(s) 532
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 9 cut(s) 18, 113, 228, 409, 487, 765, 915, 1156, 1244
Mox20I TGGCCA 1 cut(s) 826
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 1 cut(s) 1226
MscI TGGCCA 1 cut(s) 826
MseI TTAA 5 cut(s) 569, 788, 1043, 1173, 1182
MslI CAYNNNNRTG 3 cut(s) 339, 617, 1148
Msp20I TGGCCA 1 cut(s) 826
MspI CCGG 3 cut(s) 26, 140, 232
MspR9I CCNGG 3 cut(s) 141, 313, 1115
Mva1269I GAATGC 1 cut(s) 1055
MvaI CCWGG 2 cut(s) 313, 1115
MwoI GCNNNNNNNGC 4 cut(s) 22, 710, 802, 1012
NaeI GCCGGC 1 cut(s) 27
NciI CCSGG 1 cut(s) 141
NcoI CCATGG 2 cut(s) 681, 1099
NdeII GATC 7 cut(s) 118, 205, 607, 842, 952, 1095, 1192
NgoMIV GCCGGC 1 cut(s) 25
NlaIII CATG 6 cut(s) 351, 685, 861, 918, 1015, 1103
NmeAIII GCCGAG 2 cut(s) 463, 1241
NmuCI GTSAC 1 cut(s) 332
OliI CACNNNNGTG 1 cut(s) 339
PctI GAATGC 1 cut(s) 1055
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 1226
PfeI GAWTC 1 cut(s) 515
PkrI GCNGC 8 cut(s) 138, 284, 393, 436, 712, 902, 1136, 1258
PleI GAGTC 1 cut(s) 531
PpsI GAGTC 1 cut(s) 531
Psp1406I AACGTT 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 311, 1113
PspFI CCCAGC 1 cut(s) 1076
PspGI CCWGG 2 cut(s) 311, 1113
PspPI GGNCC 1 cut(s) 1254
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 1 cut(s) 952
RsaI GTAC 2 cut(s) 429, 1206
RsaNI GTAC 2 cut(s) 428, 1205
RseI CAYNNNNRTG 3 cut(s) 339, 617, 1148
SaqAI TTAA 5 cut(s) 569, 788, 1043, 1173, 1182
SatI GCNGC 8 cut(s) 137, 283, 392, 435, 711, 901, 1135, 1257
Sau3AI GATC 7 cut(s) 118, 205, 607, 842, 952, 1095, 1192
Sau96I GGNCC 1 cut(s) 1254
SchI GAGTC 1 cut(s) 532
ScrFI CCNGG 3 cut(s) 141, 313, 1115
SduI GDGCHC 1 cut(s) 1248
SexAI ACCWGGT 1 cut(s) 1113
SfaNI GCATC 2 cut(s) 76, 877
SfcI CTRYAG 1 cut(s) 1125
SmiMI CAYNNNNRTG 3 cut(s) 339, 617, 1148
Sse9I AATT 2 cut(s) 40, 725
SsiI CCGC 4 cut(s) 136, 303, 901, 1257
SspMI CTAG 2 cut(s) 981, 1241
StyD4I CCNGG 3 cut(s) 139, 311, 1113
StyI CCWWGG 5 cut(s) 48, 581, 681, 1099, 1210
TaaI ACNGT 6 cut(s) 155, 326, 496, 590, 822, 1156
TaiI ACGT 2 cut(s) 246, 1251
TaqI TCGA 1 cut(s) 877
TasI AATT 2 cut(s) 40, 725
TatI WGTACW 2 cut(s) 427, 1204
TauI GCSGC 3 cut(s) 139, 903, 1259
TfiI GAWTC 1 cut(s) 515
Tru1I TTAA 5 cut(s) 569, 788, 1043, 1173, 1182
Tru9I TTAA 5 cut(s) 569, 788, 1043, 1173, 1182
TscAI CASTG 2 cut(s) 418, 501
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 5 cut(s) 282, 391, 434, 710, 1134
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 5 cut(s) 46, 213, 336, 387, 947
TspGWI ACGGA 1 cut(s) 508
TspRI CASTG 2 cut(s) 418, 501
XbaI TCTAGA 1 cut(s) 1240
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 1226
XspI CTAG 2 cut(s) 981, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.