FvH4_2g19960

Photosystem I reaction center subunit IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010941
Physical Location & Seq
Forward (+)
16834840 .. 16836565
1726 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19960.t1

Sequence Viewer

Length: 597 bp
ATGTTGAAGGATCAGATAATTTGTTCCCAGACGCACAGAAACACTTGGGATAATTCATTCAAAGCCTCTCAATCTTATTTCCCTTGTCCTTTCTTTAATCTAATTTCCCCCCACCACTTAAGTTCATCACACACAAAGAAAGAGACCAACCTTGACACATTTGAGCAAACAATGGCTAGCTGCAGCATGGCATCAGCTGCCTCAGGCTTTGTGGTCACACCTAATGTTGCAACCAGCAACACTGCCTCGGCTAGCCGGACTAGCATGTTGTTTTTCTCTTCCAAGAGCAACATCAATGCCAGAACTAGCAGCGGCAGGTTTGTGATCAGGGCAGCCGAGGAAGGAGCAGCGCCACCCGCCGCAGCCACCAAGGCCCCCGAAGCAAAGCCTAAGCCACCACCAATTGGACCCAAGAGAGGCACTAAGGTGAAGATTCTTAGAAGGGAGTCCTACTGGTTCAAAGGCATTGGATCTGTTGTAGCTGTGGATCAGGACCCCAACACCCGCTACCCAGTCGTGGTTCGATTCAACAAAGTTAACTACGCAAATGTGTCCACAAATAACTATGCATTGGACGAGATTGAAGAAGTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PSI_PsaE

Protein Analysis

199

Amino Acids

21.71

Weight (kDa)

9.62

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaE PF02427 138 - 197 3.8e-29 Photosystem I reaction centre subunit IV / PsaE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20260 AT4G28750
fragaria_vesca FvH4_2g19960 FvH4_2g19960
malus_domestica MD05G1183900.v1.1 MD10G1171800.v1.1
prunus_persica Prupe.8G210000_v2.0.a1 Prupe.8G210000_v2.0.a1
pyrus_communis pycom05g16890 pycom10g14830
rosa_chinensis RchiOBHm_Chr6g0285621
rosa_laevigata RLG00000012621
rosa_multiflora Rmu_sc0001356.1_g000024
rosa_roxburghii Rroxscaffold_7G00182880
rosa_rugosa Rorug06G0176200
rosa_samantha Rh6AG287600 Rh6BG289000 Rh6CG290200 Rh6DG282900
rosa_wichuraiana Rw6G024730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 306
AccB7I CCANNNNNTGG 1 cut(s) 404
AciI CCGC 4 cut(s) 312, 357, 360, 505
AclWI GGATC 3 cut(s) 18, 478, 495
AfiI CCNNNNNNNGG 3 cut(s) 404, 416, 517
AflII CTTAAG 1 cut(s) 118
AgsI TTSAA 5 cut(s) 7, 61, 460, 529, 584
AleI CACNNNNGTG 1 cut(s) 425
AluBI AGCT 3 cut(s) 180, 197, 482
AluI AGCT 3 cut(s) 180, 197, 482
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 3 cut(s) 18, 478, 495
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 7 cut(s) 180, 183, 197, 309, 332, 347, 362
AspLEI GCGC 1 cut(s) 352
AspS9I GGNCC 3 cut(s) 373, 407, 493
AsuHPI GGTGA 1 cut(s) 439
AsuNHI GCTAGC 2 cut(s) 176, 251
AvaII GGWCC 2 cut(s) 407, 493
AxyI CCTNAGG 1 cut(s) 202
BbvI GCAGC 7 cut(s) 167, 184, 195, 321, 344, 359, 374
BclI TGATCA 1 cut(s) 324
BcoDI GTCTC 1 cut(s) 137
BfaI CTAG 4 cut(s) 177, 252, 261, 306
BfmI CTRYAG 1 cut(s) 181
BfoI RGCGCY 1 cut(s) 353
BfrI CTTAAG 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 306
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 9 cut(s) 181, 184, 198, 310, 313, 333, 348, 360, 363
BlsI GCNGC 9 cut(s) 182, 185, 199, 311, 314, 334, 349, 361, 364
Bme18I GGWCC 2 cut(s) 407, 493
BmgT120I GGNCC 3 cut(s) 373, 407, 493
BmiI GGNNCC 3 cut(s) 375, 409, 495
BmrI ACTGGG 1 cut(s) 506
BmsI GCATC 1 cut(s) 200
BmtI GCTAGC 2 cut(s) 180, 255
BmuI ACTGGG 1 cut(s) 506
Bpu10I CCTNAGC 1 cut(s) 390
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 3 cut(s) 246, 336, 369
Bsc4I CCNNNNNNNGG 3 cut(s) 404, 416, 517
Bse1I ACTGG 2 cut(s) 458, 512
Bse21I CCTNAGG 1 cut(s) 202
BseDI CCNNGG 3 cut(s) 246, 336, 369
BseLI CCNNNNNNNGG 3 cut(s) 404, 416, 517
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 2 cut(s) 458, 512
BseXI GCAGC 7 cut(s) 167, 184, 195, 321, 344, 359, 374
BshFI GGCC 1 cut(s) 374
BsiSI CCGG 1 cut(s) 256
BslI CCNNNNNNNGG 3 cut(s) 404, 416, 517
BsmAI GTCTC 1 cut(s) 137
BsnI GGCC 1 cut(s) 374
Bso31I GGTCTC 1 cut(s) 137
Bsp143I GATC 4 cut(s) 10, 324, 470, 487
BspACI CCGC 4 cut(s) 312, 357, 360, 505
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 1 cut(s) 215
BspLI GGNNCC 3 cut(s) 375, 409, 495
BspMAI CTGCAG 1 cut(s) 185
BspMI ACCTGC 1 cut(s) 306
BspOI GCTAGC 2 cut(s) 180, 255
BspPI GGATC 3 cut(s) 18, 478, 495
BspTI CTTAAG 1 cut(s) 118
BspTNI GGTCTC 1 cut(s) 137
BsrI ACTGG 2 cut(s) 458, 512
BssECI CCNNGG 3 cut(s) 246, 336, 369
BssMI GATC 4 cut(s) 10, 324, 470, 487
BssT1I CCWWGG 1 cut(s) 369
Bst6I CTCTTC 1 cut(s) 283
BstAFI CTTAAG 1 cut(s) 118
BstAPI GCANNNNNTGC 1 cut(s) 197
BstC8I GCNNGC 2 cut(s) 178, 253
BstDEI CTNAG 4 cut(s) 202, 390, 423, 437
BstH2I RGCGCY 1 cut(s) 353
BstHHI GCGC 1 cut(s) 352
BstKTI GATC 4 cut(s) 13, 327, 473, 490
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 4 cut(s) 10, 324, 470, 487
BstMWI GCNNNNNNNGC 5 cut(s) 197, 261, 356, 371, 380
BstNSI RCATGY 1 cut(s) 268
BstSFI CTRYAG 1 cut(s) 181
BstV1I GCAGC 7 cut(s) 167, 184, 195, 321, 344, 359, 374
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
Bsu36I CCTNAGG 1 cut(s) 202
BsuRI GGCC 1 cut(s) 374
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
BveI ACCTGC 1 cut(s) 306
Cac8I GCNNGC 2 cut(s) 178, 253
CfoI GCGC 1 cut(s) 352
Cfr13I GGNCC 3 cut(s) 373, 407, 493
CseI GACGC 1 cut(s) 40
CviAII CATG 2 cut(s) 187, 265
DdeI CTNAG 4 cut(s) 202, 390, 423, 437
DpnI GATC 4 cut(s) 12, 326, 472, 489
DpnII GATC 4 cut(s) 10, 324, 470, 487
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 137
Eco47I GGWCC 2 cut(s) 407, 493
Eco81I CCTNAGG 1 cut(s) 202
EcoO109I RGGNCCY 2 cut(s) 373, 493
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 2 cut(s) 190, 268
FaiI YATR 3 cut(s) 188, 266, 567
FatI CATG 2 cut(s) 186, 264
FauI CCCGC 2 cut(s) 364, 512
FbaI TGATCA 1 cut(s) 324
Fnu4HI GCNGC 9 cut(s) 181, 184, 198, 310, 313, 333, 348, 360, 363
Fsp4HI GCNGC 9 cut(s) 181, 184, 198, 310, 313, 333, 348, 360, 363
FspBI CTAG 4 cut(s) 177, 252, 261, 306
GlaI GCGC 1 cut(s) 351
GluI GCNGC 9 cut(s) 181, 184, 198, 310, 313, 333, 348, 360, 363
HaeII RGCGCY 1 cut(s) 353
HaeIII GGCC 1 cut(s) 374
HapII CCGG 1 cut(s) 256
HgaI GACGC 1 cut(s) 40
HhaI GCGC 1 cut(s) 352
Hin1II CATG 2 cut(s) 190, 268
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 3 cut(s) 433, 446, 525
HpaI GTTAAC 1 cut(s) 538
HpaII CCGG 1 cut(s) 256
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 2 cut(s) 538, 555
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 1 cut(s) 491
Hpy8I GTNNAC 2 cut(s) 538, 555
HpyAV CCTTC 2 cut(s) 335, 435
HpyCH4V TGCA 3 cut(s) 183, 230, 569
HpyF10VI GCNNNNNNNGC 5 cut(s) 197, 261, 356, 371, 380
HpyF3I CTNAG 4 cut(s) 202, 390, 423, 437
Hsp92II CATG 2 cut(s) 190, 268
HspAI GCGC 1 cut(s) 350
Ksp22I TGATCA 1 cut(s) 324
KspAI GTTAAC 1 cut(s) 538
Kzo9I GATC 4 cut(s) 10, 324, 470, 487
LmnI GCTCC 1 cut(s) 344
Lsp1109I GCAGC 7 cut(s) 167, 184, 195, 321, 344, 359, 374
LweI GCATC 1 cut(s) 200
MaeI CTAG 4 cut(s) 177, 252, 261, 306
MaeIII GTNAC 1 cut(s) 214
MalI GATC 4 cut(s) 12, 326, 472, 489
MboI GATC 4 cut(s) 10, 324, 470, 487
MboII GAAGA 3 cut(s) 270, 442, 596
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 1 cut(s) 470
MluCI AATT 4 cut(s) 18, 52, 102, 402
MlyI GAGTC 1 cut(s) 455
MnlI CCTC 5 cut(s) 76, 211, 256, 331, 410
Mph1103I ATGCAT 1 cut(s) 571
MseI TTAA 4 cut(s) 96, 119, 537, 591
MslI CAYNNNNRTG 1 cut(s) 425
MspA1I CMGCKG 2 cut(s) 197, 312
MspCI CTTAAG 1 cut(s) 118
MspI CCGG 1 cut(s) 256
MunI CAATTG 1 cut(s) 402
MwoI GCNNNNNNNGC 5 cut(s) 197, 261, 356, 371, 380
NdeII GATC 4 cut(s) 10, 324, 470, 487
NheI GCTAGC 2 cut(s) 176, 251
NlaIII CATG 2 cut(s) 190, 268
NlaIV GGNNCC 3 cut(s) 375, 409, 495
NmeAIII GCCGAG 2 cut(s) 227, 361
NmuCI GTSAC 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 571
NspI RCATGY 1 cut(s) 268
OliI CACNNNNGTG 1 cut(s) 425
PfeI GAWTC 2 cut(s) 433, 525
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 9 cut(s) 182, 185, 199, 311, 314, 334, 349, 361, 364
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
PpuMI RGGWCCY 1 cut(s) 493
Psp5II RGGWCCY 1 cut(s) 493
PspN4I GGNNCC 3 cut(s) 375, 409, 495
PspPI GGNCC 3 cut(s) 373, 407, 493
PspPPI RGGWCCY 1 cut(s) 493
PstI CTGCAG 1 cut(s) 185
PsuI RGATCY 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 197
RseI CAYNNNNRTG 1 cut(s) 425
SaqAI TTAA 4 cut(s) 96, 119, 537, 591
SatI GCNGC 9 cut(s) 181, 184, 198, 310, 313, 333, 348, 360, 363
Sau3AI GATC 4 cut(s) 10, 324, 470, 487
Sau96I GGNCC 3 cut(s) 373, 407, 493
SchI GAGTC 1 cut(s) 455
SetI ASST 7 cut(s) 153, 182, 199, 223, 320, 429, 484
SfaNI GCATC 1 cut(s) 200
SfcI CTRYAG 1 cut(s) 181
SinI GGWCC 2 cut(s) 407, 493
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
Sse9I AATT 4 cut(s) 18, 52, 102, 402
SsiI CCGC 4 cut(s) 312, 357, 360, 505
SspMI CTAG 4 cut(s) 177, 252, 261, 306
StyI CCWWGG 1 cut(s) 369
TaqI TCGA 1 cut(s) 523
TasI AATT 4 cut(s) 18, 52, 102, 402
TauI GCSGC 2 cut(s) 315, 362
TfiI GAWTC 2 cut(s) 433, 525
Tru1I TTAA 4 cut(s) 96, 119, 537, 591
Tru9I TTAA 4 cut(s) 96, 119, 537, 591
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 7 cut(s) 180, 183, 197, 309, 332, 347, 362
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 45, 114
TspRI CASTG 1 cut(s) 247
Van91I CCANNNNNTGG 1 cut(s) 404
Vha464I CTTAAG 1 cut(s) 118
VpaK11BI GGWCC 2 cut(s) 407, 493
XceI RCATGY 1 cut(s) 268
XspI CTAG 4 cut(s) 177, 252, 261, 306
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.