MD05G1183900.v1.1

Photosystem I reaction center subunit IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010941
Physical Location & Seq
Forward (+)
31248703 .. 31250926
2224 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1183900.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGCTAGCTGTGGCATCGCATCAGCTGCCTCAGGCTTTGTGGTAGCACCAAATGTTGCAACCACCAACGCAGGCTCAACTTCTAGCCGAAATAGCATGCTGTTTTTCCCATCAAAACCCAACACCAACACCAGAAGCAATGCTAGGTTTGTGACCAGGTCAGCAGAGGAAGCCGCTTCTCCGACGGCAGCCACCAAGACTGCACCAGAGGGGGAAGCCACCCCTAAGCCTAAGCCACCACCAATTGGACCCAAGAGAGGCACTAAGGTGAAGATTCTGAGGAAGGAATCCTACTGGTTCAAAGGCATTGGATCTGTTGTAGCTGTGGATCAGGATCCGAATACCCGTTACCCGGTCGTGGTCCGGTTCAACAAAGTTAACTATGCCAATGTGTCAACAAATAACTATGCTTTGGACGAGATTGAAGAAGTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PSI_PsaE

Protein Analysis

146

Amino Acids

15.47

Weight (kDa)

9.83

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaE PF02427 85 - 144 6.3e-30 Photosystem I reaction centre subunit IV / PsaE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20260 AT4G28750
fragaria_vesca FvH4_2g19960 FvH4_2g19960
malus_domestica MD05G1183900.v1.1 MD10G1171800.v1.1
prunus_persica Prupe.8G210000_v2.0.a1 Prupe.8G210000_v2.0.a1
pyrus_communis pycom05g16890 pycom10g14830
rosa_chinensis RchiOBHm_Chr6g0285621
rosa_laevigata RLG00000012621
rosa_multiflora Rmu_sc0001356.1_g000024
rosa_roxburghii Rroxscaffold_7G00182880
rosa_rugosa Rorug06G0176200
rosa_samantha Rh6AG287600 Rh6BG289000 Rh6CG290200 Rh6DG282900
rosa_wichuraiana Rw6G024730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 245
AciI CCGC 1 cut(s) 174
AclWI GGATC 4 cut(s) 319, 329, 336, 342
AfiI CCNNNNNNNGG 4 cut(s) 245, 257, 352, 358
AgsI TTSAA 3 cut(s) 301, 370, 425
AjnI CCWGG 1 cut(s) 155
AleI CACNNNNGTG 1 cut(s) 266
AluBI AGCT 3 cut(s) 9, 26, 323
AluI AGCT 3 cut(s) 9, 26, 323
AlwI GGATC 4 cut(s) 319, 329, 336, 342
ApeKI GCWGC 2 cut(s) 26, 188
AspS9I GGNCC 2 cut(s) 248, 361
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 1 cut(s) 280
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 2 cut(s) 248, 361
AxyI CCTNAGG 1 cut(s) 31
BamHI GGATCC 1 cut(s) 334
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
BbvI GCAGC 2 cut(s) 13, 200
BccI CCATC 1 cut(s) 118
BceAI ACGGC 1 cut(s) 201
BciT130I CCWGG 1 cut(s) 157
BcnI CCSGG 1 cut(s) 353
BfaI CTAG 3 cut(s) 6, 84, 144
BisI GCNGC 3 cut(s) 27, 174, 189
BlsI GCNGC 3 cut(s) 28, 175, 190
Bme1390I CCNGG 2 cut(s) 157, 353
Bme18I GGWCC 2 cut(s) 248, 361
BmgT120I GGNCC 2 cut(s) 248, 361
BmiI GGNNCC 2 cut(s) 250, 336
BmrFI CCNGG 2 cut(s) 157, 353
BmsI GCATC 2 cut(s) 24, 29
BmtI GCTAGC 1 cut(s) 9
Bpu10I CCTNAGC 2 cut(s) 225, 231
BpuMI CCSGG 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 363
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 257, 352, 358
Bse1I ACTGG 1 cut(s) 299
Bse21I CCTNAGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 145
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 1 cut(s) 157
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 4 cut(s) 245, 257, 352, 358
BseMI GCAATG 1 cut(s) 145
BseMII CTCAG 2 cut(s) 45, 269
BseNI ACTGG 1 cut(s) 299
BseXI GCAGC 2 cut(s) 13, 200
BsgI GTGCAG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 357
BsiEI CGRYCG 1 cut(s) 357
BsiSI CCGG 2 cut(s) 353, 364
BslI CCNNNNNNNGG 4 cut(s) 245, 257, 352, 358
Bsp143I GATC 3 cut(s) 311, 328, 334
BspACI CCGC 1 cut(s) 174
BspCNI CTCAG 2 cut(s) 44, 270
BspLI GGNNCC 2 cut(s) 250, 336
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 4 cut(s) 319, 329, 336, 342
BsrDI GCAATG 1 cut(s) 145
BsrI ACTGG 1 cut(s) 299
BssMI GATC 3 cut(s) 311, 328, 334
Bst2UI CCWGG 1 cut(s) 157
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 7, 73, 98
BstDEI CTNAG 5 cut(s) 31, 225, 231, 264, 278
BstKTI GATC 3 cut(s) 314, 331, 337
BstMBI GATC 3 cut(s) 311, 328, 334
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 3 cut(s) 26, 93, 170
BstNI CCWGG 1 cut(s) 157
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 2 cut(s) 155, 351
BstV1I GCAGC 2 cut(s) 13, 200
BstX2I RGATCY 2 cut(s) 311, 334
BstYI RGATCY 2 cut(s) 311, 334
Bsu36I CCTNAGG 1 cut(s) 31
Cac8I GCNNGC 3 cut(s) 7, 73, 98
Cfr13I GGNCC 2 cut(s) 248, 361
CsiI ACCWGGT 1 cut(s) 155
CviAII CATG 1 cut(s) 97
DdeI CTNAG 5 cut(s) 31, 225, 231, 264, 278
DpnI GATC 3 cut(s) 313, 330, 336
DpnII GATC 3 cut(s) 311, 328, 334
Eco47I GGWCC 2 cut(s) 248, 361
Eco81I CCTNAGG 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 155
FaeI CATG 1 cut(s) 100
FaiI YATR 3 cut(s) 98, 384, 408
FatI CATG 1 cut(s) 96
Fnu4HI GCNGC 3 cut(s) 27, 174, 189
Fsp4HI GCNGC 3 cut(s) 27, 174, 189
FspBI CTAG 3 cut(s) 6, 84, 144
GluI GCNGC 3 cut(s) 27, 174, 189
HapII CCGG 2 cut(s) 353, 364
Hin1II CATG 1 cut(s) 100
HincII GTYRAC 2 cut(s) 379, 396
HindII GTYRAC 2 cut(s) 379, 396
HinfI GANTC 2 cut(s) 274, 287
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 2 cut(s) 353, 364
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 2 cut(s) 379, 396
Hpy188I TCNGA 3 cut(s) 183, 279, 339
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 2 cut(s) 379, 396
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 1 cut(s) 277
HpyCH4V TGCA 2 cut(s) 59, 203
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 93, 170
HpyF3I CTNAG 5 cut(s) 31, 225, 231, 264, 278
Hsp92II CATG 1 cut(s) 100
KspAI GTTAAC 1 cut(s) 379
Kzo9I GATC 3 cut(s) 311, 328, 334
Lsp1109I GCAGC 2 cut(s) 13, 200
LweI GCATC 2 cut(s) 24, 29
MabI ACCWGGT 1 cut(s) 155
MaeI CTAG 3 cut(s) 6, 84, 144
MaeIII GTNAC 2 cut(s) 151, 347
MalI GATC 3 cut(s) 313, 330, 336
MboI GATC 3 cut(s) 311, 328, 334
MboII GAAGA 2 cut(s) 283, 437
MfeI CAATTG 1 cut(s) 243
MflI RGATCY 2 cut(s) 311, 334
MluCI AATT 1 cut(s) 243
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 5 cut(s) 40, 160, 202, 251, 273
MseI TTAA 2 cut(s) 378, 432
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 2 cut(s) 353, 364
MspR9I CCNGG 2 cut(s) 157, 353
MunI CAATTG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 157
MwoI GCNNNNNNNGC 3 cut(s) 26, 93, 170
NciI CCSGG 1 cut(s) 353
NdeII GATC 3 cut(s) 311, 328, 334
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 1 cut(s) 100
NlaIV GGNNCC 2 cut(s) 250, 336
NmuCI GTSAC 1 cut(s) 151
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 266
PaeI GCATGC 1 cut(s) 100
PfeI GAWTC 2 cut(s) 274, 287
PflFI GACNNNGTC 1 cut(s) 157
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 3 cut(s) 28, 175, 190
Psp6I CCWGG 1 cut(s) 155
PspGI CCWGG 1 cut(s) 155
PspN4I GGNNCC 2 cut(s) 250, 336
PspPI GGNCC 2 cut(s) 248, 361
PsuI RGATCY 2 cut(s) 311, 334
PsyI GACNNNGTC 1 cut(s) 157
PvuII CAGCTG 1 cut(s) 26
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 2 cut(s) 378, 432
SatI GCNGC 3 cut(s) 27, 174, 189
Sau3AI GATC 3 cut(s) 311, 328, 334
Sau96I GGNCC 2 cut(s) 248, 361
ScrFI CCNGG 2 cut(s) 157, 353
SetI ASST 6 cut(s) 11, 28, 149, 161, 270, 325
SexAI ACCWGGT 1 cut(s) 155
SfaNI GCATC 2 cut(s) 24, 29
SinI GGWCC 2 cut(s) 248, 361
SmiMI CAYNNNNRTG 1 cut(s) 266
SphI GCATGC 1 cut(s) 100
Sse9I AATT 1 cut(s) 243
SsiI CCGC 1 cut(s) 174
SspMI CTAG 3 cut(s) 6, 84, 144
StyD4I CCNGG 2 cut(s) 155, 351
TasI AATT 1 cut(s) 243
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 2 cut(s) 274, 287
Tru1I TTAA 2 cut(s) 378, 432
Tru9I TTAA 2 cut(s) 378, 432
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 2 cut(s) 26, 188
Tsp45I GTSAC 1 cut(s) 151
Tth111I GACNNNGTC 1 cut(s) 157
Van91I CCANNNNNTGG 1 cut(s) 245
VpaK11BI GGWCC 2 cut(s) 248, 361
XceI RCATGY 1 cut(s) 100
XspI CTAG 3 cut(s) 6, 84, 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.