MD10G1171800.v1.1

Photosystem I reaction center subunit IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010941
Physical Location & Seq
Forward (+)
26440987 .. 26442869
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1171800.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGCTAGCTGTGGCATGGCATCAGCTGCCTCAGGCTTTGTGGTAGCACCAAACGTTGCAACCACCAACTCAGGCTCAGCTTCCAGCCGGAGTAGCATGCTGTTTTTCCCTTCAAAACCCAACACCAACACCAGAAGCAATGCCAGGTTTGTGATCAGGGCAGCTGAAGAAGCCGCTGCGCCTCCGGCCGCCACCACGACTGCACCAGAGGGGGCAGCTACCCCTAAGCCTAAGCCACCACCAATTGGACCCAAGAGAGGCACTAAGGTGAAGATTCTTAGGAGGGAATCCTACTGGTTCAAAGGCATTGGATCTGTTGTGGCTGTGGATCAGGATCCGAATACCCGTTACCCTGTCGTGGTCCGATTCAACAAAGTTAACTATGCCAATGTGTCCACAAATAACTATGCTTTGGACGAGATTGAAGAAGTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PSI_PsaE

Protein Analysis

146

Amino Acids

15.36

Weight (kDa)

9.94

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaE PF02427 85 - 144 1.9e-29 Photosystem I reaction centre subunit IV / PsaE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20260 AT4G28750
fragaria_vesca FvH4_2g19960 FvH4_2g19960
malus_domestica MD05G1183900.v1.1 MD10G1171800.v1.1
prunus_persica Prupe.8G210000_v2.0.a1 Prupe.8G210000_v2.0.a1
pyrus_communis pycom05g16890 pycom10g14830
rosa_chinensis RchiOBHm_Chr6g0285621
rosa_laevigata RLG00000012621
rosa_multiflora Rmu_sc0001356.1_g000024
rosa_roxburghii Rroxscaffold_7G00182880
rosa_rugosa Rorug06G0176200
rosa_samantha Rh6AG287600 Rh6BG289000 Rh6CG290200 Rh6DG282900
rosa_wichuraiana Rw6G024730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 245
AciI CCGC 2 cut(s) 174, 189
AclI AACGTT 1 cut(s) 54
AclWI GGATC 4 cut(s) 319, 329, 336, 342
AcoI YGGCCR 1 cut(s) 186
AcuI CTGAAG 1 cut(s) 186
AfiI CCNNNNNNNGG 3 cut(s) 245, 257, 358
AgsI TTSAA 4 cut(s) 114, 301, 370, 425
AjnI CCWGG 1 cut(s) 143
AleI CACNNNNGTG 1 cut(s) 266
AluBI AGCT 5 cut(s) 9, 26, 80, 164, 218
AluI AGCT 5 cut(s) 9, 26, 80, 164, 218
AlwI GGATC 4 cut(s) 319, 329, 336, 342
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 4 cut(s) 26, 161, 176, 215
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 2 cut(s) 248, 361
AsuHPI GGTGA 1 cut(s) 280
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 2 cut(s) 248, 361
AxyI CCTNAGG 1 cut(s) 31
BamHI GGATCC 1 cut(s) 334
BbvI GCAGC 4 cut(s) 13, 163, 173, 227
BciT130I CCWGG 1 cut(s) 145
BclI TGATCA 1 cut(s) 153
BfaI CTAG 1 cut(s) 6
BisI GCNGC 6 cut(s) 27, 162, 174, 177, 189, 216
BlpI GCTNAGC 1 cut(s) 76
BlsI GCNGC 6 cut(s) 28, 163, 175, 178, 190, 217
Bme1390I CCNGG 1 cut(s) 145
Bme18I GGWCC 2 cut(s) 248, 361
BmgT120I GGNCC 2 cut(s) 248, 361
BmiI GGNNCC 2 cut(s) 250, 336
BmrFI CCNGG 1 cut(s) 145
BmsI GCATC 1 cut(s) 29
BmtI GCTAGC 1 cut(s) 9
Bpu10I CCTNAGC 2 cut(s) 225, 231
Bpu1102I GCTNAGC 1 cut(s) 76
BsaBI GATNNNNATC 1 cut(s) 333
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 257, 358
Bse1I ACTGG 1 cut(s) 299
Bse21I CCTNAGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 145
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 1 cut(s) 145
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 3 cut(s) 245, 257, 358
BseMI GCAATG 1 cut(s) 145
BseMII CTCAG 3 cut(s) 45, 84, 90
BseNI ACTGG 1 cut(s) 299
BseX3I CGGCCG 1 cut(s) 186
BseXI GCAGC 4 cut(s) 13, 163, 173, 227
BsgI GTGCAG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 189
BshFI GGCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 189
BsiSI CCGG 2 cut(s) 88, 185
BslI CCNNNNNNNGG 3 cut(s) 245, 257, 358
BsnI GGCC 1 cut(s) 188
Bsp143I GATC 4 cut(s) 153, 311, 328, 334
Bsp1720I GCTNAGC 1 cut(s) 76
BspACI CCGC 2 cut(s) 174, 189
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 3 cut(s) 44, 83, 89
BspLI GGNNCC 2 cut(s) 250, 336
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 4 cut(s) 319, 329, 336, 342
BsrDI GCAATG 1 cut(s) 145
BsrI ACTGG 1 cut(s) 299
BssMI GATC 4 cut(s) 153, 311, 328, 334
Bst2UI CCWGG 1 cut(s) 145
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 7, 98
BstDEI CTNAG 7 cut(s) 31, 70, 76, 225, 231, 264, 278
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 4 cut(s) 156, 314, 331, 337
BstMBI GATC 4 cut(s) 153, 311, 328, 334
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 4 cut(s) 26, 93, 170, 185
BstNI CCWGG 1 cut(s) 145
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 143
BstV1I GCAGC 4 cut(s) 13, 163, 173, 227
BstX2I RGATCY 2 cut(s) 311, 334
BstYI RGATCY 2 cut(s) 311, 334
BstZI CGGCCG 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 31
BsuRI GGCC 1 cut(s) 188
Cac8I GCNNGC 2 cut(s) 7, 98
CfoI GCGC 1 cut(s) 181
Cfr13I GGNCC 2 cut(s) 248, 361
CviAII CATG 2 cut(s) 16, 97
DdeI CTNAG 7 cut(s) 31, 70, 76, 225, 231, 264, 278
DpnI GATC 4 cut(s) 155, 313, 330, 336
DpnII GATC 4 cut(s) 153, 311, 328, 334
EaeI YGGCCR 1 cut(s) 186
EagI CGGCCG 1 cut(s) 186
EclXI CGGCCG 1 cut(s) 186
Eco47I GGWCC 2 cut(s) 248, 361
Eco52I CGGCCG 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 186
Eco81I CCTNAGG 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 143
FaeI CATG 2 cut(s) 19, 100
FaiI YATR 4 cut(s) 17, 98, 384, 408
FatI CATG 2 cut(s) 15, 96
FbaI TGATCA 1 cut(s) 153
Fnu4HI GCNGC 6 cut(s) 27, 162, 174, 177, 189, 216
Fsp4HI GCNGC 6 cut(s) 27, 162, 174, 177, 189, 216
FspBI CTAG 1 cut(s) 6
GlaI GCGC 1 cut(s) 180
GluI GCNGC 6 cut(s) 27, 162, 174, 177, 189, 216
HaeIII GGCC 1 cut(s) 188
HapII CCGG 2 cut(s) 88, 185
HhaI GCGC 1 cut(s) 181
Hin1II CATG 2 cut(s) 19, 100
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 3 cut(s) 274, 287, 366
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 2 cut(s) 88, 185
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 2 cut(s) 379, 396
Hpy188I TCNGA 2 cut(s) 339, 365
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 2 cut(s) 379, 396
HpyAV CCTTC 1 cut(s) 120
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 2 cut(s) 59, 203
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 93, 170, 185
HpyF3I CTNAG 7 cut(s) 31, 70, 76, 225, 231, 264, 278
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 2 cut(s) 19, 100
HspAI GCGC 1 cut(s) 179
Ksp22I TGATCA 1 cut(s) 153
KspAI GTTAAC 1 cut(s) 379
Kzo9I GATC 4 cut(s) 153, 311, 328, 334
Lsp1109I GCAGC 4 cut(s) 13, 163, 173, 227
LweI GCATC 1 cut(s) 29
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 347
MalI GATC 4 cut(s) 155, 313, 330, 336
MboI GATC 4 cut(s) 153, 311, 328, 334
MboII GAAGA 3 cut(s) 179, 283, 437
MfeI CAATTG 1 cut(s) 243
MflI RGATCY 2 cut(s) 311, 334
MluCI AATT 1 cut(s) 243
MnlI CCTC 5 cut(s) 40, 192, 202, 251, 276
MseI TTAA 2 cut(s) 378, 432
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 3 cut(s) 26, 164, 176
MspI CCGG 2 cut(s) 88, 185
MspR9I CCNGG 1 cut(s) 145
MunI CAATTG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 145
MwoI GCNNNNNNNGC 4 cut(s) 26, 93, 170, 185
NdeII GATC 4 cut(s) 153, 311, 328, 334
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 19, 100
NlaIV GGNNCC 2 cut(s) 250, 336
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 266
PaeI GCATGC 1 cut(s) 100
PfeI GAWTC 3 cut(s) 274, 287, 366
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 6 cut(s) 28, 163, 175, 178, 190, 217
Psp1406I AACGTT 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 2 cut(s) 250, 336
PspPI GGNCC 2 cut(s) 248, 361
PsuI RGATCY 2 cut(s) 311, 334
PvuII CAGCTG 2 cut(s) 26, 164
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 2 cut(s) 378, 432
SatI GCNGC 6 cut(s) 27, 162, 174, 177, 189, 216
Sau3AI GATC 4 cut(s) 153, 311, 328, 334
Sau96I GGNCC 2 cut(s) 248, 361
ScrFI CCNGG 1 cut(s) 145
SetI ASST 8 cut(s) 11, 28, 57, 82, 149, 166, 220, 270
SfaNI GCATC 1 cut(s) 29
SinI GGWCC 2 cut(s) 248, 361
SmiMI CAYNNNNRTG 1 cut(s) 266
SphI GCATGC 1 cut(s) 100
Sse9I AATT 1 cut(s) 243
SsiI CCGC 2 cut(s) 174, 189
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 143
TaiI ACGT 1 cut(s) 57
TasI AATT 1 cut(s) 243
TauI GCSGC 2 cut(s) 176, 191
TfiI GAWTC 3 cut(s) 274, 287, 366
Tru1I TTAA 2 cut(s) 378, 432
Tru9I TTAA 2 cut(s) 378, 432
TseI GCWGC 4 cut(s) 26, 161, 176, 215
Van91I CCANNNNNTGG 1 cut(s) 245
VpaK11BI GGWCC 2 cut(s) 248, 361
XceI RCATGY 1 cut(s) 100
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.