RLG00000012621

Photosystem I reaction center subunit IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010941
Physical Location & Seq
Reverse (-)
20601422 .. 20602444
1023 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012621

Sequence Viewer

Length: 438 bp
ATGGCTAGCTGCAGCATGGCATCAGCTGCCTCTGGCTTTGTGGTCAACCCAAATGTTGCAACCAACTCAGGCTCCGCTACTAGCCGGAGTAGTATGTTGTTTTTCTCTTCCAAGAGCAACACCAACGCCAGAACTAACAGTGGCAGGTTTGTGATCAGGGCAGCCGAGGAAGGAGCAGCGCCGCCCGCCGCAGCCCCCAAGGCCCCCGAAGGTGCAGCTGCCACAAAGCCTAAGCCACCACCAATTGGACCCAAGAGAGGCACCAAGGTGAAGATTCTTAGGAGGGAGTCCTACTGGTTCAAAGGCATTGGCTCTGTTGTAGCTGTGGATCAGGACCCAAACACCCGTTACCCAGTCGTGGTTCGATTCAACAAAGTTAACTACGCAAATGTGTCCACAAATAACTATGCGTTGGACGAGATTGAAGAAGTTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PSI_PsaE

Protein Analysis

146

Amino Acids

15.32

Weight (kDa)

9.94

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaE PF02427 85 - 144 1.9e-29 Photosystem I reaction centre subunit IV / PsaE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20260 AT4G28750
fragaria_vesca FvH4_2g19960 FvH4_2g19960
malus_domestica MD05G1183900.v1.1 MD10G1171800.v1.1
prunus_persica Prupe.8G210000_v2.0.a1 Prupe.8G210000_v2.0.a1
pyrus_communis pycom05g16890 pycom10g14830
rosa_chinensis RchiOBHm_Chr6g0285621
rosa_laevigata RLG00000012621
rosa_multiflora Rmu_sc0001356.1_g000024
rosa_roxburghii Rroxscaffold_7G00182880
rosa_rugosa Rorug06G0176200
rosa_samantha Rh6AG287600 Rh6BG289000 Rh6CG290200 Rh6DG282900
rosa_wichuraiana Rw6G024730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 245
AciI CCGC 4 cut(s) 75, 182, 186, 189
AclWI GGATC 1 cut(s) 336
AfiI CCNNNNNNNGG 3 cut(s) 245, 257, 358
AgsI TTSAA 3 cut(s) 301, 370, 425
AleI CACNNNNGTG 1 cut(s) 266
AluBI AGCT 4 cut(s) 9, 26, 218, 323
AluI AGCT 4 cut(s) 9, 26, 218, 323
AlwI GGATC 1 cut(s) 336
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 8 cut(s) 9, 12, 26, 161, 176, 191, 215, 218
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 3 cut(s) 202, 248, 334
AsuHPI GGTGA 1 cut(s) 280
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 2 cut(s) 248, 334
BanI GGYRCC 1 cut(s) 260
BbvI GCAGC 7 cut(s) 13, 24, 173, 188, 203, 205, 227
BclI TGATCA 1 cut(s) 153
BfaI CTAG 2 cut(s) 6, 81
BfmI CTRYAG 1 cut(s) 10
BfoI RGCGCY 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 135
BglI GCCNNNNNGGC 1 cut(s) 200
Bme18I GGWCC 2 cut(s) 248, 334
BmgT120I GGNCC 3 cut(s) 202, 248, 334
BmiI GGNNCC 5 cut(s) 73, 204, 250, 262, 336
BmrI ACTGGG 1 cut(s) 347
BmsI GCATC 1 cut(s) 29
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 347
Bpu10I CCTNAGC 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 165, 198, 264
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 257, 358
Bse1I ACTGG 2 cut(s) 299, 353
BseDI CCNNGG 3 cut(s) 165, 198, 264
BseLI CCNNNNNNNGG 3 cut(s) 245, 257, 358
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 2 cut(s) 299, 353
BseXI GCAGC 7 cut(s) 13, 24, 173, 188, 203, 205, 227
BsgI GTGCAG 1 cut(s) 234
BshFI GGCC 1 cut(s) 203
BshNI GGYRCC 1 cut(s) 260
BsiSI CCGG 1 cut(s) 85
BslI CCNNNNNNNGG 3 cut(s) 245, 257, 358
BsnI GGCC 1 cut(s) 203
Bsp143I GATC 2 cut(s) 153, 328
BspACI CCGC 4 cut(s) 75, 182, 186, 189
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 1 cut(s) 80
BspLI GGNNCC 5 cut(s) 73, 204, 250, 262, 336
BspMAI CTGCAG 1 cut(s) 14
BspMI ACCTGC 1 cut(s) 135
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 260
BsrI ACTGG 2 cut(s) 299, 353
BssECI CCNNGG 3 cut(s) 165, 198, 264
BssMI GATC 2 cut(s) 153, 328
BssT1I CCWWGG 2 cut(s) 198, 264
Bst4CI ACNGT 1 cut(s) 140
Bst6I CTCTTC 1 cut(s) 112
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 7, 186
BstDEI CTNAG 3 cut(s) 67, 231, 278
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 2 cut(s) 156, 331
BstMBI GATC 2 cut(s) 153, 328
BstMWI GCNNNNNNNGC 3 cut(s) 26, 185, 200
BstSFI CTRYAG 1 cut(s) 10
BstV1I GCAGC 7 cut(s) 13, 24, 173, 188, 203, 205, 227
BsuRI GGCC 1 cut(s) 203
BtsIMutI CAGTG 1 cut(s) 145
BveI ACCTGC 1 cut(s) 135
Cac8I GCNNGC 2 cut(s) 7, 186
CfoI GCGC 1 cut(s) 181
Cfr13I GGNCC 3 cut(s) 202, 248, 334
CviAII CATG 1 cut(s) 16
DdeI CTNAG 3 cut(s) 67, 231, 278
DpnI GATC 2 cut(s) 155, 330
DpnII GATC 2 cut(s) 153, 328
Eam1104I CTCTTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 112
Eco130I CCWWGG 2 cut(s) 198, 264
Eco47I GGWCC 2 cut(s) 248, 334
EcoO109I RGGNCCY 2 cut(s) 202, 334
EcoT14I CCWWGG 2 cut(s) 198, 264
ErhI CCWWGG 2 cut(s) 198, 264
FaeI CATG 1 cut(s) 19
FaiI YATR 3 cut(s) 17, 95, 408
FatI CATG 1 cut(s) 15
FauI CCCGC 1 cut(s) 193
FbaI TGATCA 1 cut(s) 153
FspBI CTAG 2 cut(s) 6, 81
GlaI GCGC 1 cut(s) 180
HaeII RGCGCY 1 cut(s) 182
HaeIII GGCC 1 cut(s) 203
HapII CCGG 1 cut(s) 85
HhaI GCGC 1 cut(s) 181
Hin1II CATG 1 cut(s) 19
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HincII GTYRAC 3 cut(s) 46, 379, 433
HindII GTYRAC 3 cut(s) 46, 379, 433
HinfI GANTC 3 cut(s) 274, 287, 366
HpaI GTTAAC 2 cut(s) 379, 433
HpaII CCGG 1 cut(s) 85
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 4 cut(s) 46, 379, 396, 433
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 4 cut(s) 46, 379, 396, 433
HpyAV CCTTC 2 cut(s) 164, 203
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4V TGCA 3 cut(s) 12, 59, 215
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 185, 200
HpyF3I CTNAG 3 cut(s) 67, 231, 278
Hsp92II CATG 1 cut(s) 19
HspAI GCGC 1 cut(s) 179
Ksp22I TGATCA 1 cut(s) 153
KspAI GTTAAC 2 cut(s) 379, 433
Kzo9I GATC 2 cut(s) 153, 328
LmnI GCTCC 2 cut(s) 77, 173
LpnPI CCDG 9 cut(s) 18, 54, 98, 130, 142, 142, 280, 317, 366
Lsp1109I GCAGC 7 cut(s) 13, 24, 173, 188, 203, 205, 227
LweI GCATC 1 cut(s) 29
MaeI CTAG 2 cut(s) 6, 81
MaeIII GTNAC 1 cut(s) 347
MalI GATC 2 cut(s) 155, 330
MboI GATC 2 cut(s) 153, 328
MboII GAAGA 3 cut(s) 99, 283, 437
MfeI CAATTG 1 cut(s) 243
MluCI AATT 1 cut(s) 243
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 393
MnlI CCTC 4 cut(s) 40, 160, 251, 276
MseI TTAA 2 cut(s) 378, 432
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 2 cut(s) 26, 218
MspI CCGG 1 cut(s) 85
MunI CAATTG 1 cut(s) 243
MwoI GCNNNNNNNGC 3 cut(s) 26, 185, 200
NdeII GATC 2 cut(s) 153, 328
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 1 cut(s) 19
NlaIV GGNNCC 5 cut(s) 73, 204, 250, 262, 336
NmeAIII GCCGAG 1 cut(s) 190
OliI CACNNNNGTG 1 cut(s) 266
PfeI GAWTC 2 cut(s) 274, 366
PflMI CCANNNNNTGG 1 cut(s) 245
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 334
Psp5II RGGWCCY 1 cut(s) 334
PspN4I GGNNCC 5 cut(s) 73, 204, 250, 262, 336
PspPI GGNCC 3 cut(s) 202, 248, 334
PspPPI RGGWCCY 1 cut(s) 334
PstI CTGCAG 1 cut(s) 14
PvuII CAGCTG 2 cut(s) 26, 218
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 2 cut(s) 378, 432
Sau3AI GATC 2 cut(s) 153, 328
Sau96I GGNCC 3 cut(s) 202, 248, 334
SchI GAGTC 1 cut(s) 296
SetI ASST 7 cut(s) 11, 28, 149, 214, 220, 270, 325
SfaNI GCATC 1 cut(s) 29
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 2 cut(s) 248, 334
SmiMI CAYNNNNRTG 1 cut(s) 266
Sse9I AATT 1 cut(s) 243
SsiI CCGC 4 cut(s) 75, 182, 186, 189
SspMI CTAG 2 cut(s) 6, 81
StyI CCWWGG 2 cut(s) 198, 264
TaaI ACNGT 1 cut(s) 140
TaqI TCGA 1 cut(s) 364
TasI AATT 1 cut(s) 243
TauI GCSGC 2 cut(s) 184, 191
TfiI GAWTC 2 cut(s) 274, 366
Tru1I TTAA 2 cut(s) 378, 432
Tru9I TTAA 2 cut(s) 378, 432
TscAI CASTG 1 cut(s) 145
TseI GCWGC 8 cut(s) 9, 12, 26, 161, 176, 191, 215, 218
TspRI CASTG 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 245
VpaK11BI GGWCC 2 cut(s) 248, 334
XspI CTAG 2 cut(s) 6, 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.