RchiOBHm_Chr6g0285621

Photosystem I reaction center subunit IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010941
Physical Location & Seq
Forward (+)
48956528 .. 48958179
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25620

Sequence Viewer

Length: 438 bp
ATGGCTAGCTGCAGCATGGCATCAGCTGCCTCAGGCTTTGTGGTCACGCCAAATGTTGCAACCAACTCAGGCTCCGCTACTAGCCGGAGTAGCATGTTGTTTTTCTCGTCCAAGAGCAACACCAACGCCAGAACTAACAGTGGCAGGTTTGTGATCAGGGCAGCCGAGGAAGGAGCAGGGCCGCCCGCCGCAGCCCCCAAGGCCCCCGAAGGTGCAGCTGCCACAAAGCCTAAGCCACCACCAATTGGACCCAAGAGAGGCACCAAGGTGAAGATTCTTAGGAGGGAGTCCTACTGGTTCAAAGGCATTGGCTCTGTTGTAGCTGTGGATCAGGACCCAAACACCCGTTACCCAGTCGTGGTTCGATTCAACAAAGTTAACTACGCAAATGTGTCCACAAATAACTATGCGTTGGACGAGATTGAAGAAGTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PSI_PsaE

Protein Analysis

145

Amino Acids

15.31

Weight (kDa)

10.0

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaE PF02427 85 - 144 1.9e-29 Photosystem I reaction centre subunit IV / PsaE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20260 AT4G28750
fragaria_vesca FvH4_2g19960 FvH4_2g19960
malus_domestica MD05G1183900.v1.1 MD10G1171800.v1.1
prunus_persica Prupe.8G210000_v2.0.a1 Prupe.8G210000_v2.0.a1
pyrus_communis pycom05g16890 pycom10g14830
rosa_chinensis RchiOBHm_Chr6g0285621
rosa_laevigata RLG00000012621
rosa_multiflora Rmu_sc0001356.1_g000024
rosa_roxburghii Rroxscaffold_7G00182880
rosa_rugosa Rorug06G0176200
rosa_samantha Rh6AG287600 Rh6BG289000 Rh6CG290200 Rh6DG282900
rosa_wichuraiana Rw6G024730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 245
AciI CCGC 4 cut(s) 75, 182, 186, 189
AclWI GGATC 1 cut(s) 336
AfiI CCNNNNNNNGG 3 cut(s) 245, 257, 358
AgsI TTSAA 3 cut(s) 301, 370, 425
AleI CACNNNNGTG 1 cut(s) 266
AluBI AGCT 4 cut(s) 9, 26, 218, 323
AluI AGCT 4 cut(s) 9, 26, 218, 323
AlwI GGATC 1 cut(s) 336
AoxI GGCC 2 cut(s) 179, 201
ApeKI GCWGC 7 cut(s) 9, 12, 26, 161, 191, 215, 218
AspS9I GGNCC 4 cut(s) 179, 202, 248, 334
AsuHPI GGTGA 1 cut(s) 280
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 2 cut(s) 248, 334
AxyI CCTNAGG 1 cut(s) 31
BanI GGYRCC 1 cut(s) 260
BbvI GCAGC 6 cut(s) 13, 24, 173, 203, 205, 227
BclI TGATCA 1 cut(s) 153
BfaI CTAG 2 cut(s) 6, 81
BfmI CTRYAG 1 cut(s) 10
BfuAI ACCTGC 1 cut(s) 135
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 9 cut(s) 10, 13, 27, 162, 182, 189, 192, 216, 219
BlsI GCNGC 9 cut(s) 11, 14, 28, 163, 183, 190, 193, 217, 220
Bme18I GGWCC 2 cut(s) 248, 334
BmgT120I GGNCC 4 cut(s) 179, 202, 248, 334
BmiI GGNNCC 5 cut(s) 73, 204, 250, 262, 336
BmrI ACTGGG 1 cut(s) 347
BmsI GCATC 1 cut(s) 29
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 347
Bpu10I CCTNAGC 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 165, 198, 264
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 257, 358
Bse1I ACTGG 2 cut(s) 299, 353
Bse21I CCTNAGG 1 cut(s) 31
BseDI CCNNGG 3 cut(s) 165, 198, 264
BseLI CCNNNNNNNGG 3 cut(s) 245, 257, 358
BseMII CTCAG 2 cut(s) 45, 81
BseNI ACTGG 2 cut(s) 299, 353
BseXI GCAGC 6 cut(s) 13, 24, 173, 203, 205, 227
BsgI GTGCAG 1 cut(s) 234
BshFI GGCC 2 cut(s) 181, 203
BshNI GGYRCC 1 cut(s) 260
BsiSI CCGG 1 cut(s) 85
BslI CCNNNNNNNGG 3 cut(s) 245, 257, 358
BsnI GGCC 2 cut(s) 181, 203
Bsp143I GATC 2 cut(s) 153, 328
BspACI CCGC 4 cut(s) 75, 182, 186, 189
BspANI GGCC 2 cut(s) 181, 203
BspCNI CTCAG 2 cut(s) 44, 80
BspLI GGNNCC 5 cut(s) 73, 204, 250, 262, 336
BspMAI CTGCAG 1 cut(s) 14
BspMI ACCTGC 1 cut(s) 135
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 260
BsrI ACTGG 2 cut(s) 299, 353
BssECI CCNNGG 3 cut(s) 165, 198, 264
BssMI GATC 2 cut(s) 153, 328
BssT1I CCWWGG 2 cut(s) 198, 264
Bst4CI ACNGT 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 7, 186
BstDEI CTNAG 4 cut(s) 31, 67, 231, 278
BstKTI GATC 2 cut(s) 156, 331
BstMBI GATC 2 cut(s) 153, 328
BstMWI GCNNNNNNNGC 3 cut(s) 26, 90, 200
BstNSI RCATGY 1 cut(s) 97
BstSFI CTRYAG 1 cut(s) 10
BstV1I GCAGC 6 cut(s) 13, 24, 173, 203, 205, 227
Bsu36I CCTNAGG 1 cut(s) 31
BsuRI GGCC 2 cut(s) 181, 203
BtsIMutI CAGTG 1 cut(s) 145
BveI ACCTGC 1 cut(s) 135
Cac8I GCNNGC 2 cut(s) 7, 186
Cfr13I GGNCC 4 cut(s) 179, 202, 248, 334
CviAII CATG 2 cut(s) 16, 94
DdeI CTNAG 4 cut(s) 31, 67, 231, 278
DpnI GATC 2 cut(s) 155, 330
DpnII GATC 2 cut(s) 153, 328
Eco130I CCWWGG 2 cut(s) 198, 264
Eco47I GGWCC 2 cut(s) 248, 334
Eco81I CCTNAGG 1 cut(s) 31
EcoO109I RGGNCCY 2 cut(s) 202, 334
EcoT14I CCWWGG 2 cut(s) 198, 264
ErhI CCWWGG 2 cut(s) 198, 264
FaeI CATG 2 cut(s) 19, 97
FaiI YATR 3 cut(s) 17, 95, 408
FatI CATG 2 cut(s) 15, 93
FauI CCCGC 1 cut(s) 193
FbaI TGATCA 1 cut(s) 153
Fnu4HI GCNGC 9 cut(s) 10, 13, 27, 162, 182, 189, 192, 216, 219
Fsp4HI GCNGC 9 cut(s) 10, 13, 27, 162, 182, 189, 192, 216, 219
FspBI CTAG 2 cut(s) 6, 81
GluI GCNGC 9 cut(s) 10, 13, 27, 162, 182, 189, 192, 216, 219
HaeIII GGCC 2 cut(s) 181, 203
HapII CCGG 1 cut(s) 85
Hin1II CATG 2 cut(s) 19, 97
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 3 cut(s) 274, 287, 366
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 1 cut(s) 85
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 2 cut(s) 379, 396
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 2 cut(s) 379, 396
HpyAV CCTTC 2 cut(s) 164, 203
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4V TGCA 3 cut(s) 12, 59, 215
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 90, 200
HpyF3I CTNAG 4 cut(s) 31, 67, 231, 278
Hsp92II CATG 2 cut(s) 19, 97
Ksp22I TGATCA 1 cut(s) 153
KspAI GTTAAC 1 cut(s) 379
Kzo9I GATC 2 cut(s) 153, 328
LmnI GCTCC 2 cut(s) 77, 173
Lsp1109I GCAGC 6 cut(s) 13, 24, 173, 203, 205, 227
LweI GCATC 1 cut(s) 29
MaeI CTAG 2 cut(s) 6, 81
MaeIII GTNAC 2 cut(s) 43, 347
MalI GATC 2 cut(s) 155, 330
MboI GATC 2 cut(s) 153, 328
MboII GAAGA 2 cut(s) 283, 437
MfeI CAATTG 1 cut(s) 243
MluCI AATT 1 cut(s) 243
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 393
MnlI CCTC 4 cut(s) 40, 160, 251, 276
MseI TTAA 2 cut(s) 378, 432
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 2 cut(s) 26, 218
MspI CCGG 1 cut(s) 85
MunI CAATTG 1 cut(s) 243
MwoI GCNNNNNNNGC 3 cut(s) 26, 90, 200
NdeII GATC 2 cut(s) 153, 328
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 19, 97
NlaIV GGNNCC 5 cut(s) 73, 204, 250, 262, 336
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 97
OliI CACNNNNGTG 1 cut(s) 266
PfeI GAWTC 2 cut(s) 274, 366
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 9 cut(s) 11, 14, 28, 163, 183, 190, 193, 217, 220
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 334
Psp5II RGGWCCY 1 cut(s) 334
PspN4I GGNNCC 5 cut(s) 73, 204, 250, 262, 336
PspPI GGNCC 4 cut(s) 179, 202, 248, 334
PspPPI RGGWCCY 1 cut(s) 334
PstI CTGCAG 1 cut(s) 14
PvuII CAGCTG 2 cut(s) 26, 218
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 2 cut(s) 378, 432
SatI GCNGC 9 cut(s) 10, 13, 27, 162, 182, 189, 192, 216, 219
Sau3AI GATC 2 cut(s) 153, 328
Sau96I GGNCC 4 cut(s) 179, 202, 248, 334
SchI GAGTC 1 cut(s) 296
SetI ASST 7 cut(s) 11, 28, 149, 214, 220, 270, 325
SfaNI GCATC 1 cut(s) 29
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 2 cut(s) 248, 334
SmiMI CAYNNNNRTG 1 cut(s) 266
Sse9I AATT 1 cut(s) 243
SsiI CCGC 4 cut(s) 75, 182, 186, 189
SspMI CTAG 2 cut(s) 6, 81
StyI CCWWGG 2 cut(s) 198, 264
TaaI ACNGT 1 cut(s) 140
TaqI TCGA 1 cut(s) 364
TasI AATT 1 cut(s) 243
TauI GCSGC 2 cut(s) 184, 191
TfiI GAWTC 2 cut(s) 274, 366
Tru1I TTAA 2 cut(s) 378, 432
Tru9I TTAA 2 cut(s) 378, 432
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 7 cut(s) 9, 12, 26, 161, 191, 215, 218
Tsp45I GTSAC 1 cut(s) 43
TspRI CASTG 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 245
VpaK11BI GGWCC 2 cut(s) 248, 334
XceI RCATGY 1 cut(s) 97
XspI CTAG 2 cut(s) 6, 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.