FvH4_2g20900

Rho GDP-dissociation inhibitor 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014663
Physical Location & Seq
Reverse (-)
17437780 .. 17439928
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20900.t1

Sequence Viewer

Length: 678 bp
ATGGAGGGTGGGAAGAGAGGAGATGAAGCAGGCCCATCATCAGAAAAGCTAGGGGAACTGAGTGAAAAGCTAAATGAGGTCTGTCATGTTGGAGAAGATGAAGAAGATGAAGATGAAGATCACAGGAGTAATGGGGTTGCTGCTGGCTTTGTTCCTGGGCCTTTGGTCTCTCTCAAGGAGCAAATTGAGAAAGACAAGGATGATGACAGCTTACGGAGGTGGAAGCAGAAGCTGCTTGGTTGCTTGGAAAGCGATTCGGATGGCCAAATGGAACCTGAAGTCAAATTCCACTCCATCAGTATCGTCTCCGATGATTTTGGGGAAATAAATACTCCATTGCCCGTTGATGAAAATCAGATTGGCCGTGTGCTGTTTACACTCAAAGAAGGATCTGAATATCGGCTTAAGCTCACATTTAGTGTTCTGCACAACATTGTTTCTGGCCTTACCTACTCCAACACAGTGTGGAAAGGAGGACTCCAAGTTGATCAAAGCAAAGGAATGTTGGGTACATTTGCTCCAAATAGAGAACCCTATGAGCACACCTTGGAAGAGGAGACCACTCCATCTGGGGTGCTTGCAAGGGGACTTTACTCGGCGAGGCTTAAGTTTGAAGACGATGACAAGAGATGTTATATGGAACTGAAATACTCCTTTGAGATCAAAAGGCAGAGTTAG

Protein Analysis

226

Amino Acids

25.28

Weight (kDa)

4.71

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 35 - 223 7e-71 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014663)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20900
malus_domestica MD05G1192500.v1.1 MD10G1179000.v1.1
prunus_persica Prupe.8G218800_v2.0.a1 Prupe.8G218800_v2.0.a1
pyrus_communis pycom05g17950
rosa_chinensis RchiOBHm_Chr6g0287301
rosa_laevigata RLG00000012503
rosa_multiflora Rmu_sc0013803.1_g000016
rosa_roxburghii Rroxscaffold_7G00181560
rosa_rugosa Rorug06G0186700
rosa_samantha Rh6AG298100 Rh6BG303600 Rh6CG306400 Rh6CG311700 Rh6DG295300
rosa_wichuraiana Rw6G025770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 397
AcoI YGGCCR 2 cut(s) 262, 361
AcsI RAATTY 1 cut(s) 284
AcuI CTGAAG 1 cut(s) 297
AdeI CACNNNGTG 1 cut(s) 465
AfaI GTAC 1 cut(s) 511
AflII CTTAAG 2 cut(s) 404, 605
AgsI TTSAA 1 cut(s) 614
AjnI CCWGG 1 cut(s) 154
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 5 cut(s) 49, 70, 210, 232, 409
AluI AGCT 5 cut(s) 49, 70, 210, 232, 409
Alw21I GWGCWC 1 cut(s) 543
Alw26I GTCTC 3 cut(s) 172, 310, 551
AlwI GGATC 1 cut(s) 397
AlwNI CAGNNNCTG 1 cut(s) 232
AoxI GGCC 5 cut(s) 31, 158, 262, 361, 442
ApeKI GCWGC 2 cut(s) 140, 232
ApoI RAATTY 1 cut(s) 284
AspS9I GGNCC 2 cut(s) 32, 158
BalI TGGCCA 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 621
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 2 cut(s) 127, 219
BccI CCATC 4 cut(s) 43, 254, 302, 574
BceAI ACGGC 1 cut(s) 348
BciT130I CCWGG 1 cut(s) 156
BclI TGATCA 1 cut(s) 487
BcoDI GTCTC 3 cut(s) 172, 310, 551
BfaI CTAG 1 cut(s) 50
BfrI CTTAAG 2 cut(s) 404, 605
BisI GCNGC 2 cut(s) 141, 233
BlsI GCNGC 2 cut(s) 142, 234
Bme1390I CCNGG 1 cut(s) 156
BmgT120I GGNCC 2 cut(s) 32, 158
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 1 cut(s) 156
BpiI GAAGAC 1 cut(s) 621
BpuEI CTTGAG 1 cut(s) 158
BsaBI GATNNNNATC 2 cut(s) 117, 351
BsaI GGTCTC 2 cut(s) 172, 551
BsaJI CCNNGG 2 cut(s) 155, 546
BsaXI ACNNNNNCTCC 2 cut(s) 502, 532
Bse3DI GCAATG 1 cut(s) 335
Bse8I GATNNNNATC 2 cut(s) 117, 351
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 155, 546
BseGI GGATG 2 cut(s) 205, 265
BseJI GATNNNNATC 2 cut(s) 117, 351
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 1 cut(s) 50
BseRI GAGGAG 2 cut(s) 33, 569
BseXI GCAGC 2 cut(s) 127, 219
BsgI GTGCAG 1 cut(s) 410
BshFI GGCC 5 cut(s) 33, 160, 264, 363, 444
BsiHKAI GWGCWC 1 cut(s) 543
BslFI GGGAC 1 cut(s) 600
BsmAI GTCTC 3 cut(s) 172, 310, 551
BsmBI CGTCTC 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 600
BsnI GGCC 5 cut(s) 33, 160, 264, 363, 444
Bso31I GGTCTC 2 cut(s) 172, 551
Bsp1286I GDGCHC 1 cut(s) 543
Bsp143I GATC 4 cut(s) 118, 389, 487, 660
BspANI GGCC 5 cut(s) 33, 160, 264, 363, 444
BspCNI CTCAG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 273
BspPI GGATC 1 cut(s) 397
BspTI CTTAAG 2 cut(s) 404, 605
BspTNI GGTCTC 2 cut(s) 172, 551
BsrDI GCAATG 1 cut(s) 335
BssECI CCNNGG 2 cut(s) 155, 546
BssMI GATC 4 cut(s) 118, 389, 487, 660
BssT1I CCWWGG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 2 cut(s) 8, 546
BstAFI CTTAAG 2 cut(s) 404, 605
BstAPI GCANNNNNTGC 1 cut(s) 232
BstC8I GCNNGC 3 cut(s) 31, 145, 579
BstDEI CTNAG 1 cut(s) 59
BstF5I GGATG 2 cut(s) 205, 265
BstKTI GATC 4 cut(s) 121, 392, 490, 663
BstMAI GTCTC 3 cut(s) 172, 310, 551
BstMBI GATC 4 cut(s) 118, 389, 487, 660
BstMWI GCNNNNNNNGC 2 cut(s) 232, 249
BstNI CCWGG 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 154
BstV1I GCAGC 2 cut(s) 127, 219
BstV2I GAAGAC 1 cut(s) 621
BstX2I RGATCY 1 cut(s) 389
BstYI RGATCY 1 cut(s) 389
BsuRI GGCC 5 cut(s) 33, 160, 264, 363, 444
BtsCI GGATG 2 cut(s) 205, 265
BtsIMutI CAGTG 1 cut(s) 468
Cac8I GCNNGC 3 cut(s) 31, 145, 579
CaiI CAGNNNCTG 1 cut(s) 232
Cfr13I GGNCC 2 cut(s) 32, 158
Csp6I GTAC 1 cut(s) 510
CviAII CATG 1 cut(s) 86
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 1 cut(s) 59
DpnI GATC 4 cut(s) 120, 391, 489, 662
DpnII GATC 4 cut(s) 118, 389, 487, 660
DraIII CACNNNGTG 1 cut(s) 465
EaeI YGGCCR 2 cut(s) 262, 361
Eam1104I CTCTTC 2 cut(s) 8, 546
EarI CTCTTC 2 cut(s) 8, 546
Eco130I CCWWGG 1 cut(s) 546
Eco31I GGTCTC 2 cut(s) 172, 551
Eco57I CTGAAG 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 546
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 1 cut(s) 89
FaiI YATR 4 cut(s) 87, 537, 636, 638
FaqI GGGAC 1 cut(s) 600
FatI CATG 1 cut(s) 85
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 2 cut(s) 141, 233
FokI GGATG 2 cut(s) 212, 272
Fsp4HI GCNGC 2 cut(s) 141, 233
FspBI CTAG 1 cut(s) 50
GluI GCNGC 2 cut(s) 141, 233
HaeIII GGCC 5 cut(s) 33, 160, 264, 363, 444
Hin1II CATG 1 cut(s) 89
HinfI GANTC 2 cut(s) 254, 477
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 5 cut(s) 43, 259, 310, 357, 394
Hpy8I GTNNAC 1 cut(s) 375
HpyAV CCTTC 1 cut(s) 380
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4V TGCA 2 cut(s) 427, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 232, 249
HpyF3I CTNAG 1 cut(s) 59
Hsp92II CATG 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 4 cut(s) 118, 389, 487, 660
LmnI GCTCC 2 cut(s) 178, 523
LpnPI CCDG 8 cut(s) 15, 109, 129, 141, 168, 288, 426, 555
Lsp1109I GCAGC 2 cut(s) 127, 219
MaeI CTAG 1 cut(s) 50
MalI GATC 4 cut(s) 120, 391, 489, 662
MboI GATC 4 cut(s) 118, 389, 487, 660
MboII GAAGA 8 cut(s) 25, 107, 113, 116, 122, 128, 563, 626
MflI RGATCY 1 cut(s) 389
MhlI GDGCHC 1 cut(s) 543
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 2 cut(s) 183, 284
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 1 cut(s) 471
MmeI TCCRAC 2 cut(s) 70, 480
MnlI CCTC 6 cut(s) 11, 70, 210, 467, 547, 594
Mox20I TGGCCA 1 cut(s) 264
MscI TGGCCA 1 cut(s) 264
MseI TTAA 2 cut(s) 405, 606
Msp20I TGGCCA 1 cut(s) 264
MspCI CTTAAG 2 cut(s) 404, 605
MspR9I CCNGG 1 cut(s) 156
MvaI CCWGG 1 cut(s) 156
MwoI GCNNNNNNNGC 2 cut(s) 232, 249
NdeII GATC 4 cut(s) 118, 389, 487, 660
NlaIII CATG 1 cut(s) 89
NlaIV GGNNCC 1 cut(s) 273
NmeAIII GCCGAG 1 cut(s) 575
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 2 cut(s) 142, 234
PleI GAGTC 1 cut(s) 471
PpsI GAGTC 1 cut(s) 471
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 2 cut(s) 32, 158
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 389
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
SaqAI TTAA 2 cut(s) 405, 606
SatI GCNGC 2 cut(s) 141, 233
Sau3AI GATC 4 cut(s) 118, 389, 487, 660
Sau96I GGNCC 2 cut(s) 32, 158
SchI GAGTC 1 cut(s) 471
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 1 cut(s) 543
SmlI CTYRAG 3 cut(s) 173, 404, 605
SmoI CTYRAG 3 cut(s) 173, 404, 605
Sse9I AATT 2 cut(s) 183, 284
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 1 cut(s) 154
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 1 cut(s) 463
TasI AATT 2 cut(s) 183, 284
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 2 cut(s) 405, 606
Tru9I TTAA 2 cut(s) 405, 606
TscAI CASTG 1 cut(s) 468
TseI GCWGC 2 cut(s) 140, 232
TspDTI ATGAA 5 cut(s) 39, 114, 123, 129, 363
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 1 cut(s) 468
Vha464I CTTAAG 2 cut(s) 404, 605
XapI RAATTY 1 cut(s) 284
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.