MD10G1179000.v1.1

Rho GDP-dissociation inhibitor 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014663
Physical Location & Seq
Reverse (-)
27274950 .. 27277212
2263 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1179000.v1.1.491

Sequence Viewer

Length: 951 bp
ATGATGAATTTAGCAGCCCTCTCCTGTTGGAGGTTTGTCTGTGTAAATCGGTCCAGTGAACAGACAGTATATACGACTTTATACGTATTTATTGCCCCCATTTCAATCATAATCCCTTCCTTTTTTATGTCATTGTTTGCTCTTTTGACTGACGCTCCCTGTGTGTCTCAGTCTGGGGACAGGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGATGGAGGGAGGGAAGAGGGCAGATGAAGCAGGGGCATCATCGTCAGGTGGGTTTTCATCAGCTGGGGCTGTAAGTGATCATGGAAGAGGAAGACAAGAAAAGCAGAAGGAAATGGCTGAAAAGCTGAGTGCTTTTTGTGATGCTGGGGGAGAAGAAGAGGAAGAAGATGATAAGAGTAATGAGGTTGCTGCTGCTGCTGGTTTTGTTCCTGGGCCTTTGGTTTCTCTCAAGGAACAGATTGAGAAAGACAAGGATGATGAAAGCTTAAGGAGGTGGAAAGAGAAACTGCTCGGTTGCTTGGAAATGGAAAGCGATTTGAATGGCCAAATGGAATCTGAAGTCAAATTTCACTCCATCGGAATCATCTCTGATGACTTTGGGGAAATCACCACTCCCTTACCTGTTGCAGAAAATCAGATCAACCGTGTCCTGTTTACATTGCAAGAGGGATCTCAATATCGCCTTAAGCTAACATTTAGCGTTCTGCACAACATTGTTTCTGGCCTTATCTACTCCAACACAGTGTGGAAGGGAGGACTTCAAGTCGATCAGAGCAAAGGAATGTTGGGTACGTTTGCTCCTAACAAAGAACCATATGTGCACACTTTGGAAGAGGAGACCACTCCATCTGGGTTGCTTGCAAGGGGCATATATGCAGCAAAGCTTAAGTTTGAAGACGATGACAGGAGATGCCATATGGAACTTCAATACTCCTTCGAGATCAAAAAGGGCAGATAG

Protein Analysis

317

Amino Acids

35.16

Weight (kDa)

4.95

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 125 - 314 3.1e-68 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014663)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20900
malus_domestica MD05G1192500.v1.1 MD10G1179000.v1.1
prunus_persica Prupe.8G218800_v2.0.a1 Prupe.8G218800_v2.0.a1
pyrus_communis pycom05g17950
rosa_chinensis RchiOBHm_Chr6g0287301
rosa_laevigata RLG00000012503
rosa_multiflora Rmu_sc0013803.1_g000016
rosa_roxburghii Rroxscaffold_7G00181560
rosa_rugosa Rorug06G0186700
rosa_samantha Rh6AG298100 Rh6BG303600 Rh6CG306400 Rh6CG311700 Rh6DG295300
rosa_wichuraiana Rw6G025770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 670
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 2 cut(s) 7, 557
AcuI CTGAAG 1 cut(s) 570
AdeI CACNNNGTG 1 cut(s) 738
AfaI GTAC 1 cut(s) 784
AfiI CCNNNNNNNGG 1 cut(s) 30
AflII CTTAAG 3 cut(s) 478, 677, 878
AgsI TTSAA 5 cut(s) 105, 532, 755, 887, 920
AhdI GACNNNNNGTC 1 cut(s) 755
AjnI CCWGG 1 cut(s) 421
AluBI AGCT 5 cut(s) 275, 337, 477, 682, 877
AluI AGCT 5 cut(s) 275, 337, 477, 682, 877
Alw21I GWGCWC 1 cut(s) 816
Alw26I GTCTC 2 cut(s) 171, 824
Alw44I GTGCAC 1 cut(s) 812
AlwI GGATC 1 cut(s) 670
AoxI GGCC 3 cut(s) 425, 535, 715
ApaLI GTGCAC 1 cut(s) 812
ApeKI GCWGC 5 cut(s) 14, 401, 404, 407, 869
ApoI RAATTY 2 cut(s) 7, 557
AspS9I GGNCC 2 cut(s) 51, 425
AsuHPI GGTGA 1 cut(s) 592
AvaII GGWCC 1 cut(s) 51
BaeGI GKGCMC 1 cut(s) 816
BalI TGGCCA 1 cut(s) 537
BbsI GAAGAC 2 cut(s) 310, 894
Bbv12I GWGCWC 1 cut(s) 816
BbvI GCAGC 5 cut(s) 26, 388, 391, 394, 881
BccI CCATC 3 cut(s) 208, 575, 847
BciT130I CCWGG 1 cut(s) 423
BclI TGATCA 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 171, 824
BfrI CTTAAG 3 cut(s) 478, 677, 878
BisI GCNGC 5 cut(s) 15, 402, 405, 408, 870
BlsI GCNGC 5 cut(s) 16, 403, 406, 409, 871
Bme1390I CCNGG 1 cut(s) 423
Bme18I GGWCC 1 cut(s) 51
BmeRI GACNNNNNGTC 1 cut(s) 755
BmgT120I GGNCC 2 cut(s) 51, 425
BmrFI CCNGG 1 cut(s) 423
BmsI GCATC 3 cut(s) 257, 343, 893
BpiI GAAGAC 2 cut(s) 310, 894
BpuEI CTTGAG 1 cut(s) 425
BsaAI YACGTR 1 cut(s) 85
BsaI GGTCTC 1 cut(s) 824
BsaJI CCNNGG 1 cut(s) 422
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 775, 805
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse1I ACTGG 1 cut(s) 54
Bse3DI GCAATG 1 cut(s) 650
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 1 cut(s) 422
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMI GCAATG 1 cut(s) 650
BseMII CTCAG 2 cut(s) 182, 329
BseNI ACTGG 1 cut(s) 54
BseRI GAGGAG 1 cut(s) 842
BseSI GKGCMC 1 cut(s) 816
BseXI GCAGC 5 cut(s) 26, 388, 391, 394, 881
BseYI CCCAGC 2 cut(s) 275, 356
BsgI GTGCAG 1 cut(s) 683
BshFI GGCC 3 cut(s) 427, 537, 717
BsiHKAI GWGCWC 1 cut(s) 816
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 2 cut(s) 171, 824
BsmFI GGGAC 1 cut(s) 191
BsnI GGCC 3 cut(s) 427, 537, 717
Bso31I GGTCTC 1 cut(s) 824
Bsp1286I GDGCHC 1 cut(s) 816
Bsp143I GATC 5 cut(s) 289, 630, 662, 760, 933
BspANI GGCC 3 cut(s) 427, 537, 717
BspCNI CTCAG 2 cut(s) 181, 330
BspPI GGATC 1 cut(s) 670
BspTI CTTAAG 3 cut(s) 478, 677, 878
BspTNI GGTCTC 1 cut(s) 824
BsrDI GCAATG 1 cut(s) 650
BsrI ACTGG 1 cut(s) 54
BssECI CCNNGG 1 cut(s) 422
BssMI GATC 5 cut(s) 289, 630, 662, 760, 933
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 3 cut(s) 67, 638, 736
Bst6I CTCTTC 4 cut(s) 221, 292, 363, 819
BstAFI CTTAAG 3 cut(s) 478, 677, 878
BstBAI YACGTR 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 852
BstDEI CTNAG 2 cut(s) 168, 338
BstENI CCTNNNNNAGG 1 cut(s) 28
BstF5I GGATG 1 cut(s) 472
BstKTI GATC 5 cut(s) 292, 633, 665, 763, 936
BstMAI GTCTC 2 cut(s) 171, 824
BstMBI GATC 5 cut(s) 289, 630, 662, 760, 933
BstMWI GCNNNNNNNGC 2 cut(s) 239, 407
BstNI CCWGG 1 cut(s) 423
BstSCI CCNGG 1 cut(s) 421
BstSLI GKGCMC 1 cut(s) 816
BstSNI TACGTA 1 cut(s) 85
BstV1I GCAGC 5 cut(s) 26, 388, 391, 394, 881
BstV2I GAAGAC 2 cut(s) 310, 894
BstX2I RGATCY 1 cut(s) 662
BstYI RGATCY 1 cut(s) 662
BsuRI GGCC 3 cut(s) 427, 537, 717
BtsCI GGATG 1 cut(s) 472
BtsIMutI CAGTG 2 cut(s) 61, 741
Cac8I GCNNGC 1 cut(s) 852
Cfr13I GGNCC 2 cut(s) 51, 425
CseI GACGC 1 cut(s) 161
Csp6I GTAC 1 cut(s) 783
CviAII CATG 1 cut(s) 293
CviQI GTAC 1 cut(s) 783
DdeI CTNAG 2 cut(s) 168, 338
DpnI GATC 5 cut(s) 291, 632, 664, 762, 935
DpnII GATC 5 cut(s) 289, 630, 662, 760, 933
DraIII CACNNNGTG 1 cut(s) 738
DriI GACNNNNNGTC 1 cut(s) 755
EaeI YGGCCR 1 cut(s) 535
Eam1104I CTCTTC 4 cut(s) 221, 292, 363, 819
Eam1105I GACNNNNNGTC 1 cut(s) 755
EarI CTCTTC 4 cut(s) 221, 292, 363, 819
Eco105I TACGTA 1 cut(s) 85
Eco31I GGTCTC 1 cut(s) 824
Eco47I GGWCC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 570
EcoNI CCTNNNNNAGG 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 421
FaeI CATG 1 cut(s) 296
FaqI GGGAC 1 cut(s) 191
FatI CATG 1 cut(s) 292
FauNDI CATATG 2 cut(s) 808, 909
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 5 cut(s) 15, 402, 405, 408, 870
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 5 cut(s) 15, 402, 405, 408, 870
GluI GCNGC 5 cut(s) 15, 402, 405, 408, 870
GsaI CCCAGC 2 cut(s) 279, 360
HaeIII GGCC 3 cut(s) 427, 537, 717
HgaI GACGC 1 cut(s) 161
Hin1II CATG 1 cut(s) 296
HindIII AAGCTT 2 cut(s) 475, 875
HinfI GANTC 2 cut(s) 545, 573
HphI GGTGA 1 cut(s) 592
Hpy166II GTNNAC 3 cut(s) 59, 648, 814
Hpy188I TCNGA 5 cut(s) 550, 572, 583, 630, 765
Hpy188III TCNNGA 1 cut(s) 931
Hpy8I GTNNAC 3 cut(s) 59, 648, 814
HpyAV CCTTC 4 cut(s) 126, 313, 736, 937
HpyCH4III ACNGT 3 cut(s) 67, 638, 736
HpyCH4IV ACGT 2 cut(s) 84, 785
HpyCH4V TGCA 6 cut(s) 620, 655, 700, 814, 854, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 407
HpyF3I CTNAG 2 cut(s) 168, 338
HpySE526I ACGT 2 cut(s) 84, 785
Hsp92II CATG 1 cut(s) 296
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 5 cut(s) 289, 630, 662, 760, 933
LmnI GCTCC 2 cut(s) 160, 796
Lsp1109I GCAGC 5 cut(s) 26, 388, 391, 394, 881
LweI GCATC 3 cut(s) 257, 343, 893
MaeII ACGT 2 cut(s) 84, 785
MalI GATC 5 cut(s) 291, 632, 664, 762, 935
MboI GATC 5 cut(s) 289, 630, 662, 760, 933
MboII GAAGA 9 cut(s) 238, 309, 315, 377, 380, 386, 389, 836, 899
MflI RGATCY 1 cut(s) 662
MhlI GDGCHC 1 cut(s) 816
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 2 cut(s) 7, 557
MluNI TGGCCA 1 cut(s) 537
MmeI TCCRAC 2 cut(s) 8, 753
Mox20I TGGCCA 1 cut(s) 537
MscI TGGCCA 1 cut(s) 537
MseI TTAA 3 cut(s) 479, 678, 879
Msp20I TGGCCA 1 cut(s) 537
MspA1I CMGCKG 1 cut(s) 275
MspCI CTTAAG 3 cut(s) 478, 677, 878
MspR9I CCNGG 1 cut(s) 423
MvaI CCWGG 1 cut(s) 423
MwoI GCNNNNNNNGC 2 cut(s) 239, 407
NdeI CATATG 2 cut(s) 808, 909
NdeII GATC 5 cut(s) 289, 630, 662, 760, 933
NlaIII CATG 1 cut(s) 296
PfeI GAWTC 2 cut(s) 545, 573
PkrI GCNGC 5 cut(s) 16, 403, 406, 409, 871
Ppu21I YACGTR 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 421
PspFI CCCAGC 2 cut(s) 275, 356
PspGI CCWGG 1 cut(s) 421
PspPI GGNCC 2 cut(s) 51, 425
PsuI RGATCY 1 cut(s) 662
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 1 cut(s) 784
RsaNI GTAC 1 cut(s) 783
SaqAI TTAA 3 cut(s) 479, 678, 879
SatI GCNGC 5 cut(s) 15, 402, 405, 408, 870
Sau3AI GATC 5 cut(s) 289, 630, 662, 760, 933
Sau96I GGNCC 2 cut(s) 51, 425
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 1 cut(s) 816
SfaNI GCATC 3 cut(s) 257, 343, 893
SinI GGWCC 1 cut(s) 51
SmlI CTYRAG 4 cut(s) 440, 478, 677, 878
SmoI CTYRAG 4 cut(s) 440, 478, 677, 878
SnaBI TACGTA 1 cut(s) 85
Sse9I AATT 2 cut(s) 7, 557
StyD4I CCNGG 1 cut(s) 421
TaaI ACNGT 3 cut(s) 67, 638, 736
TaiI ACGT 2 cut(s) 87, 788
TaqI TCGA 2 cut(s) 759, 930
TaqII GACCGA 1 cut(s) 39
TasI AATT 2 cut(s) 7, 557
TfiI GAWTC 2 cut(s) 545, 573
Tru1I TTAA 3 cut(s) 479, 678, 879
Tru9I TTAA 3 cut(s) 479, 678, 879
TscAI CASTG 2 cut(s) 61, 741
TseI GCWGC 5 cut(s) 14, 401, 404, 407, 869
TspDTI ATGAA 4 cut(s) 20, 252, 258, 486
TspRI CASTG 2 cut(s) 61, 741
Vha464I CTTAAG 3 cut(s) 478, 677, 878
VneI GTGCAC 1 cut(s) 812
VpaK11BI GGWCC 1 cut(s) 51
XagI CCTNNNNNAGG 1 cut(s) 28
XapI RAATTY 2 cut(s) 7, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.