Rh6AG298100

Rho GDP-dissociation inhibitor 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0014663
Physical Location & Seq
Reverse (-)
49691186 .. 49693561
2376 bp
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UTR
Exon/CDS
Intron
Rh6AG298100.1

Sequence Viewer

Length: 558 bp
ATGGAGGGTGGGAAGAGAGGAGATGAAGCAGGCCCATCATCAGAAAAGCTAAAGGAACTGAGTGAAAAGCTAAGTGAGTTCTGTCATGTTGAGGAAGAAGAAGAAGAAGAAGATGGTGAAGATCACAGGAGTAATGGGGTTGCTGCTGGCTTTGTTCCTGGGCCTTTGGTTTCTCTCAAGGAACAAATTGAGAAAGACAAGGATGATGACAGCTTAAGGAGGTGGAAACAGAAGCTGCTTGGTTCCTTGGAAAGCGATTTGGATGGCCAAATGGAACCTGAAGTCAAATTCCACTCAATTGGTATCATTTCCGATGACTTTGGGGAAATAAATACTCCATTGCCCGTTGATGAAAATCTGATCGGTCGTGTCCTGTTTACACTCAAAGAAGGATCTGAATATCGGCTTAAGCTCGCATTTAGTGTTCTGCACAACATTGTTTCTGGCCTTACCTATTCCAACACAGTGTGGAAAGGAGGACTTCAAGGTGCGTATTACATCCATATTGTTGCCTTTGGATGTATGAATTGTGCAATAGAAATGGTTGAAAGTTTTTGA

Protein Analysis

185

Amino Acids

20.58

Weight (kDa)

4.59

Isoelectric Point (pI)

58.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 36 - 162 3.5e-35 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014663)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20900
malus_domestica MD05G1192500.v1.1 MD10G1179000.v1.1
prunus_persica Prupe.8G218800_v2.0.a1 Prupe.8G218800_v2.0.a1
pyrus_communis pycom05g17950
rosa_chinensis RchiOBHm_Chr6g0287301
rosa_laevigata RLG00000012503
rosa_multiflora Rmu_sc0013803.1_g000016
rosa_roxburghii Rroxscaffold_7G00181560
rosa_rugosa Rorug06G0186700
rosa_samantha Rh6AG298100 Rh6BG303600 Rh6CG306400 Rh6CG311700 Rh6DG295300
rosa_wichuraiana Rw6G025770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 400
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 300
AdeI CACNNNGTG 1 cut(s) 468
AflII CTTAAG 2 cut(s) 214, 407
AgsI TTSAA 2 cut(s) 485, 548
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 5 cut(s) 49, 70, 213, 235, 412
AluI AGCT 5 cut(s) 49, 70, 213, 235, 412
AlwI GGATC 1 cut(s) 400
AlwNI CAGNNNCTG 1 cut(s) 235
AoxI GGCC 4 cut(s) 31, 161, 265, 445
ApeKI GCWGC 2 cut(s) 143, 235
ApoI RAATTY 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 32, 161
AsuHPI GGTGA 1 cut(s) 128
BalI TGGCCA 1 cut(s) 267
BbvI GCAGC 2 cut(s) 130, 222
BccI CCATC 3 cut(s) 43, 107, 257
BciT130I CCWGG 1 cut(s) 159
BfrI CTTAAG 2 cut(s) 214, 407
BisI GCNGC 2 cut(s) 144, 236
BlsI GCNGC 2 cut(s) 145, 237
Bme1390I CCNGG 1 cut(s) 159
BmgT120I GGNCC 2 cut(s) 32, 161
BmiI GGNNCC 2 cut(s) 244, 276
BmrFI CCNGG 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 161
BsaBI GATNNNNATC 1 cut(s) 354
BsaJI CCNNGG 2 cut(s) 158, 246
Bse3DI GCAATG 1 cut(s) 338
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 2 cut(s) 158, 246
BseGI GGATG 4 cut(s) 208, 268, 498, 524
BseJI GATNNNNATC 1 cut(s) 354
BseMI GCAATG 1 cut(s) 338
BseMII CTCAG 1 cut(s) 50
BseRI GAGGAG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 130, 222
BsgI GTGCAG 1 cut(s) 413
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 4 cut(s) 33, 163, 267, 447
BsiEI CGRYCG 1 cut(s) 367
BsnI GGCC 4 cut(s) 33, 163, 267, 447
Bsp143I GATC 3 cut(s) 121, 360, 392
BspANI GGCC 4 cut(s) 33, 163, 267, 447
BspCNI CTCAG 1 cut(s) 51
BspLI GGNNCC 2 cut(s) 244, 276
BspPI GGATC 1 cut(s) 400
BspTI CTTAAG 2 cut(s) 214, 407
BsrDI GCAATG 1 cut(s) 338
BssECI CCNNGG 2 cut(s) 158, 246
BssMI GATC 3 cut(s) 121, 360, 392
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 1 cut(s) 8
BstAFI CTTAAG 2 cut(s) 214, 407
BstC8I GCNNGC 3 cut(s) 31, 148, 414
BstDEI CTNAG 2 cut(s) 59, 71
BstF5I GGATG 4 cut(s) 208, 268, 498, 524
BstKTI GATC 3 cut(s) 124, 363, 395
BstMBI GATC 3 cut(s) 121, 360, 392
BstMCI CGRYCG 1 cut(s) 367
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 130, 222
BstX2I RGATCY 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 299
BstYI RGATCY 1 cut(s) 392
BsuRI GGCC 4 cut(s) 33, 163, 267, 447
BtsCI GGATG 4 cut(s) 208, 268, 498, 524
BtsIMutI CAGTG 1 cut(s) 471
Cac8I GCNNGC 3 cut(s) 31, 148, 414
CaiI CAGNNNCTG 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 32, 161
CviAII CATG 1 cut(s) 86
DdeI CTNAG 2 cut(s) 59, 71
DpnI GATC 3 cut(s) 123, 362, 394
DpnII GATC 3 cut(s) 121, 360, 392
DraIII CACNNNGTG 1 cut(s) 468
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 246
Eco57I CTGAAG 1 cut(s) 300
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 1 cut(s) 89
FaiI YATR 3 cut(s) 87, 504, 524
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FatI CATG 1 cut(s) 85
Fnu4HI GCNGC 2 cut(s) 144, 236
FokI GGATG 4 cut(s) 215, 275, 485, 531
Fsp4HI GCNGC 2 cut(s) 144, 236
GluI GCNGC 2 cut(s) 144, 236
HaeIII GGCC 4 cut(s) 33, 163, 267, 447
Hin1II CATG 1 cut(s) 89
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 4 cut(s) 43, 313, 360, 397
Hpy8I GTNNAC 1 cut(s) 378
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 2 cut(s) 430, 533
HpyF3I CTNAG 2 cut(s) 59, 71
Hsp92II CATG 1 cut(s) 89
Kzo9I GATC 3 cut(s) 121, 360, 392
LpnPI CCDG 8 cut(s) 15, 112, 132, 144, 171, 291, 386, 429
Lsp1109I GCAGC 2 cut(s) 130, 222
MalI GATC 3 cut(s) 123, 362, 394
MboI GATC 3 cut(s) 121, 360, 392
MboII GAAGA 8 cut(s) 25, 107, 110, 113, 116, 119, 122, 131
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 1 cut(s) 392
MlsI TGGCCA 1 cut(s) 267
MluCI AATT 4 cut(s) 186, 287, 297, 526
MluNI TGGCCA 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 4 cut(s) 11, 85, 213, 470
Mox20I TGGCCA 1 cut(s) 267
MscI TGGCCA 1 cut(s) 267
MseI TTAA 2 cut(s) 215, 408
Msp20I TGGCCA 1 cut(s) 267
MspCI CTTAAG 2 cut(s) 214, 407
MspR9I CCNGG 1 cut(s) 159
MunI CAATTG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 159
NdeII GATC 3 cut(s) 121, 360, 392
NlaIII CATG 1 cut(s) 89
NlaIV GGNNCC 2 cut(s) 244, 276
PkrI GCNGC 2 cut(s) 145, 237
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 2 cut(s) 244, 276
PspPI GGNCC 2 cut(s) 32, 161
PstNI CAGNNNCTG 1 cut(s) 235
PsuI RGATCY 1 cut(s) 392
SaqAI TTAA 2 cut(s) 215, 408
SatI GCNGC 2 cut(s) 144, 236
Sau3AI GATC 3 cut(s) 121, 360, 392
Sau96I GGNCC 2 cut(s) 32, 161
ScrFI CCNGG 1 cut(s) 159
SetI ASST 9 cut(s) 51, 72, 215, 224, 237, 280, 414, 455, 490
SmlI CTYRAG 3 cut(s) 176, 214, 407
SmoI CTYRAG 3 cut(s) 176, 214, 407
Sse9I AATT 4 cut(s) 186, 287, 297, 526
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 466
TaqII GACCGA 1 cut(s) 353
TasI AATT 4 cut(s) 186, 287, 297, 526
Tru1I TTAA 2 cut(s) 215, 408
Tru9I TTAA 2 cut(s) 215, 408
TscAI CASTG 1 cut(s) 471
TseI GCWGC 2 cut(s) 143, 235
TspDTI ATGAA 3 cut(s) 39, 366, 539
TspRI CASTG 1 cut(s) 471
Vha464I CTTAAG 2 cut(s) 214, 407
XapI RAATTY 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.