Rroxscaffold_7G00181560

Rho GDP-dissociation inhibitor 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014663
Physical Location & Seq
Forward (+)
20652518 .. 20654767
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00181560.1

Sequence Viewer

Length: 681 bp
ATGGAGGGTGGGAAGAGAGGAGATGAAGCAGGCCCATCATCAGAAAAGCTAAAGGAACTGAGTGAAAAGCTAAGTGAGTTCTGTCATGTTGAGGAAGAGGAAGAAGAAGAAGATGGTGAAGATCACAGGAGTAATGGGGTTGCTGCTGGCTTTGTTCCTGGGCCTTTGGTTTCTCTCAAGGAACAAATTGAGAAAGACAAGGATGATGACAGCTTAAGGAGGTGGAAACAGAAGCTGCTTGGTTCCTTGGAAAGCGATTTGGATGGCCAAATGGAACCTGAAGTCAAATTCCACTCAATTGGTATCATCTCCGATGACTTTGGGGAAATAAATACTCCATTGCCCGTTGATGAAAATCAGATCAGTTGTGTCCTGTTTACACTCAAAGAAGGATCTGAATATCGGCTTAAGCTCGCATTTAGTGTTCTGCACAACATTGTTTCTGGCCTTTCCTATTCCAACACAGTGTGGAAAGGAGGACTTCAAGTTGATCAAAGCAAAGGAATGTTGGGTACATTTGCTCCGAATAGAGAACCCTATGAGCACACCTTGGAAGAGGAGACCACTCCATCTGGGGTGCTTGCAAGGGGACTTTACTCGGCAAAGCTTAAGTTCGAAGACGATGACAAGAGATGTTATATGGAACTGAAATACTCCTTTGAGATCAAAAAGCGGAGTTAG

Protein Analysis

226

Amino Acids

25.43

Weight (kDa)

4.74

Isoelectric Point (pI)

55.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 36 - 224 1e-69 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014663)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20900
malus_domestica MD05G1192500.v1.1 MD10G1179000.v1.1
prunus_persica Prupe.8G218800_v2.0.a1 Prupe.8G218800_v2.0.a1
pyrus_communis pycom05g17950
rosa_chinensis RchiOBHm_Chr6g0287301
rosa_laevigata RLG00000012503
rosa_multiflora Rmu_sc0013803.1_g000016
rosa_roxburghii Rroxscaffold_7G00181560
rosa_rugosa Rorug06G0186700
rosa_samantha Rh6AG298100 Rh6BG303600 Rh6CG306400 Rh6CG311700 Rh6DG295300
rosa_wichuraiana Rw6G025770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 673
AclWI GGATC 1 cut(s) 400
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 300
AdeI CACNNNGTG 1 cut(s) 468
AfaI GTAC 1 cut(s) 514
AflII CTTAAG 3 cut(s) 214, 407, 608
AgsI TTSAA 1 cut(s) 485
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 6 cut(s) 49, 70, 213, 235, 412, 607
AluI AGCT 6 cut(s) 49, 70, 213, 235, 412, 607
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 1 cut(s) 400
AlwNI CAGNNNCTG 1 cut(s) 235
AoxI GGCC 4 cut(s) 31, 161, 265, 445
ApeKI GCWGC 2 cut(s) 143, 235
ApoI RAATTY 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 32, 161
AsuHPI GGTGA 1 cut(s) 128
AsuII TTCGAA 1 cut(s) 615
BalI TGGCCA 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 624
Bbv12I GWGCWC 1 cut(s) 546
BbvI GCAGC 2 cut(s) 130, 222
BccI CCATC 4 cut(s) 43, 107, 257, 577
BciT130I CCWGG 1 cut(s) 159
BclI TGATCA 1 cut(s) 490
BcoDI GTCTC 1 cut(s) 554
BfrI CTTAAG 3 cut(s) 214, 407, 608
BisI GCNGC 2 cut(s) 144, 236
BlsI GCNGC 2 cut(s) 145, 237
Bme1390I CCNGG 1 cut(s) 159
BmgT120I GGNCC 2 cut(s) 32, 161
BmiI GGNNCC 2 cut(s) 244, 276
BmrFI CCNGG 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 624
Bpu14I TTCGAA 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 161
BsaBI GATNNNNATC 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 3 cut(s) 158, 246, 549
BsaXI ACNNNNNCTCC 2 cut(s) 505, 535
Bse3DI GCAATG 1 cut(s) 338
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 3 cut(s) 158, 246, 549
BseGI GGATG 2 cut(s) 208, 268
BseJI GATNNNNATC 1 cut(s) 354
BseMI GCAATG 1 cut(s) 338
BseMII CTCAG 1 cut(s) 50
BseRI GAGGAG 2 cut(s) 33, 572
BseXI GCAGC 2 cut(s) 130, 222
BsgI GTGCAG 1 cut(s) 413
BshFI GGCC 4 cut(s) 33, 163, 267, 447
BsiHKAI GWGCWC 1 cut(s) 546
BslFI GGGAC 1 cut(s) 603
BsmAI GTCTC 1 cut(s) 554
BsmFI GGGAC 1 cut(s) 603
BsnI GGCC 4 cut(s) 33, 163, 267, 447
Bso31I GGTCTC 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 615
Bsp1286I GDGCHC 1 cut(s) 546
Bsp143I GATC 5 cut(s) 121, 360, 392, 490, 663
BspACI CCGC 1 cut(s) 673
BspANI GGCC 4 cut(s) 33, 163, 267, 447
BspCNI CTCAG 1 cut(s) 51
BspLI GGNNCC 2 cut(s) 244, 276
BspPI GGATC 1 cut(s) 400
BspT104I TTCGAA 1 cut(s) 615
BspTI CTTAAG 3 cut(s) 214, 407, 608
BspTNI GGTCTC 1 cut(s) 554
BsrDI GCAATG 1 cut(s) 338
BssECI CCNNGG 3 cut(s) 158, 246, 549
BssMI GATC 5 cut(s) 121, 360, 392, 490, 663
BssT1I CCWWGG 2 cut(s) 246, 549
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 3 cut(s) 8, 90, 549
BstAFI CTTAAG 3 cut(s) 214, 407, 608
BstBI TTCGAA 1 cut(s) 615
BstC8I GCNNGC 4 cut(s) 31, 148, 414, 582
BstDEI CTNAG 2 cut(s) 59, 71
BstF5I GGATG 2 cut(s) 208, 268
BstKTI GATC 5 cut(s) 124, 363, 395, 493, 666
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 5 cut(s) 121, 360, 392, 490, 663
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 130, 222
BstV2I GAAGAC 1 cut(s) 624
BstX2I RGATCY 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 299
BstYI RGATCY 1 cut(s) 392
BsuRI GGCC 4 cut(s) 33, 163, 267, 447
BtsCI GGATG 2 cut(s) 208, 268
BtsIMutI CAGTG 1 cut(s) 471
Cac8I GCNNGC 4 cut(s) 31, 148, 414, 582
CaiI CAGNNNCTG 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 32, 161
Csp6I GTAC 1 cut(s) 513
CviAII CATG 1 cut(s) 86
CviQI GTAC 1 cut(s) 513
DdeI CTNAG 2 cut(s) 59, 71
DpnI GATC 5 cut(s) 123, 362, 394, 492, 665
DpnII GATC 5 cut(s) 121, 360, 392, 490, 663
DraIII CACNNNGTG 1 cut(s) 468
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 3 cut(s) 8, 90, 549
EarI CTCTTC 3 cut(s) 8, 90, 549
Eco130I CCWWGG 2 cut(s) 246, 549
Eco31I GGTCTC 1 cut(s) 554
Eco57I CTGAAG 1 cut(s) 300
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 2 cut(s) 246, 549
ErhI CCWWGG 2 cut(s) 246, 549
FaeI CATG 1 cut(s) 89
FaiI YATR 4 cut(s) 87, 540, 639, 641
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FaqI GGGAC 1 cut(s) 603
FatI CATG 1 cut(s) 85
FbaI TGATCA 1 cut(s) 490
Fnu4HI GCNGC 2 cut(s) 144, 236
FokI GGATG 2 cut(s) 215, 275
Fsp4HI GCNGC 2 cut(s) 144, 236
GluI GCNGC 2 cut(s) 144, 236
HaeIII GGCC 4 cut(s) 33, 163, 267, 447
Hin1II CATG 1 cut(s) 89
HindIII AAGCTT 1 cut(s) 605
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 5 cut(s) 43, 313, 360, 397, 525
Hpy8I GTNNAC 1 cut(s) 378
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 2 cut(s) 430, 584
HpyF3I CTNAG 2 cut(s) 59, 71
Hsp92II CATG 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 5 cut(s) 121, 360, 392, 490, 663
LmnI GCTCC 1 cut(s) 526
LpnPI CCDG 9 cut(s) 15, 112, 132, 144, 171, 291, 386, 429, 558
Lsp1109I GCAGC 2 cut(s) 130, 222
MalI GATC 5 cut(s) 123, 362, 394, 492, 665
MboI GATC 5 cut(s) 121, 360, 392, 490, 663
MboII GAAGA 9 cut(s) 25, 107, 113, 116, 119, 122, 131, 566, 629
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 1 cut(s) 546
MlsI TGGCCA 1 cut(s) 267
MluCI AATT 3 cut(s) 186, 287, 297
MluNI TGGCCA 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 6 cut(s) 11, 85, 91, 213, 470, 550
Mox20I TGGCCA 1 cut(s) 267
MscI TGGCCA 1 cut(s) 267
MseI TTAA 3 cut(s) 215, 408, 609
Msp20I TGGCCA 1 cut(s) 267
MspCI CTTAAG 3 cut(s) 214, 407, 608
MspR9I CCNGG 1 cut(s) 159
MunI CAATTG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 159
NdeII GATC 5 cut(s) 121, 360, 392, 490, 663
NlaIII CATG 1 cut(s) 89
NlaIV GGNNCC 2 cut(s) 244, 276
NmeAIII GCCGAG 1 cut(s) 578
NspV TTCGAA 1 cut(s) 615
PkrI GCNGC 2 cut(s) 145, 237
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 2 cut(s) 244, 276
PspPI GGNCC 2 cut(s) 32, 161
PstNI CAGNNNCTG 1 cut(s) 235
PsuI RGATCY 1 cut(s) 392
RsaI GTAC 1 cut(s) 514
RsaNI GTAC 1 cut(s) 513
SaqAI TTAA 3 cut(s) 215, 408, 609
SatI GCNGC 2 cut(s) 144, 236
Sau3AI GATC 5 cut(s) 121, 360, 392, 490, 663
Sau96I GGNCC 2 cut(s) 32, 161
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 1 cut(s) 546
SetI ASST 9 cut(s) 51, 72, 215, 224, 237, 280, 414, 551, 609
SfuI TTCGAA 1 cut(s) 615
SmlI CTYRAG 4 cut(s) 176, 214, 407, 608
SmoI CTYRAG 4 cut(s) 176, 214, 407, 608
Sse9I AATT 3 cut(s) 186, 287, 297
SsiI CCGC 1 cut(s) 673
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 2 cut(s) 246, 549
TaaI ACNGT 1 cut(s) 466
TaqI TCGA 1 cut(s) 615
TasI AATT 3 cut(s) 186, 287, 297
Tru1I TTAA 3 cut(s) 215, 408, 609
Tru9I TTAA 3 cut(s) 215, 408, 609
TscAI CASTG 1 cut(s) 471
TseI GCWGC 2 cut(s) 143, 235
TspDTI ATGAA 2 cut(s) 39, 366
TspRI CASTG 1 cut(s) 471
Vha464I CTTAAG 3 cut(s) 214, 407, 608
XapI RAATTY 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.