FvH4_2g23260

Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014488
Physical Location & Seq
Forward (+)
19043119 .. 19047339
4221 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23260.t1

Sequence Viewer

Length: 1293 bp
ATGGAGGACACTTACGAAGAAGCCAGCAAGGACACGGAGGTCGCGCCTGCACTCATAGCAGTCCACCCGACCCAGAATTCGGTTGCTGTCGCAATCGGGTCGGACCTTCGCGTCTTCGACCTTATTGGGGGTTGTGCAGTCTCTTTGGTGGATGATTCTGGTGGGTCTCTTCATAAGGATTCCATAAGAGCCATTCGGTACGGAGCAAGTGGGAAGCTATTTGTGTCTGCTGGTGATGATAAACTTGTCAAGATATGGTCAACTGAATCTTGGCGCTGCATTAGTACTGTCTGCTCAGAGAAAAGAGTGACTGCAGTTGCCATAAGCAACGATGGTTCCTATGTTTGTTTTGCGGACAAATTTGGGGTTATTTGGGTTGTGGATCTGGATGTGGAGGGCTTTGATGGAAACCAAGTGTCTGCTAACAAAAAGGCAGCTCCTATGCTTTCTCACTATTGTAGCATAATAACAAGCCTGGAATTTTCTCCTGATGGGCGATTTTTTGTCAGTGCTGATCGGGATTTCAAAATACGCGTTAATGTGTTTCCCAAGAAGCCTTTAGATGGTGCCCATGAGATACAAAGTTTTGCGCTTGGTCATACAGAATTTGTTTCCTGCCTTGCTTTTGTTTGCACTCAGGAGTTCCCCCAAGGTTTTCTTGTCTCTGGAAGTGGTGATTCAACTGTTCGCCTATGGGATATTTCCTCAGGATCTCTTTTAGATACTTGTGATATTATAGAAAAGGCAGGACCTTTAGAGTTTAAAGGAATAGAAGAGGAGTCCTGTGCGGCTGTTACTGATTTATGCACTATCCCAGATAGTACACTTGTTGCCGTCGCTGTACAAAGTTTGCAAGGGATTATATTGTTGAGATGCGATCCTTCTGCTCAAACTCTCGATGTTGCCAAGGTGGTAACTATCAAGGGAGATACATTTATTCCTACAAGCCTGGCAACTAGTGTATCCTCAGGACTGTTGTGGATGGTAACAGGTGCCTCTAACTTGGGTGGTCTTGAAAGCCCCTCTTTGGCTCGTGTCAGGGTTATCTCTGACTTTAAGGAAACCACTCCTGATGTTGAAGGGCCAACTGTTTTGGAAGATGATGAGACCCCTGGGGGCGTGAAATTGCTCGAAAAATTACAGGGAAGAGTGTCCATTGACGAAAAGGTTTTCTCAACAGCAGCTGAGGCTTTGAAAATATCCATGAGCAAATTACTGATAAAGAAGCAGTACTCTACCGAAAGGCGAGAATTTAGAAAGAGAGGTAGAAATGATAAGAAACTCAAACAGTGA

Protein Analysis

431

Amino Acids

46.39

Weight (kDa)

5.03

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 24 - 245 1.1e-15 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 35 - 116 3e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 56 - 179 8e-09 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 56 - 185 4.3e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 57 - 130 9.9e-11 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 57 - 119 7.3e-08 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 57 - 252 4.5e-15 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 70 - 243 1.1e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 71 - 240 9e-10 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 103 - 243 5e-09 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 153 - 242 3.2e-09 WDR5 beta-propeller domain
WD40 PF00400 199 - 233 6.5e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03110
fragaria_vesca FvH4_2g23260
malus_domestica MD03G1261900.v1.1 MD11G1282600.v1.1
prunus_persica Prupe.8G238200_v2.0.a1
pyrus_communis pycom03g20960 pycom11g25030
rosa_chinensis RchiOBHm_Chr6g0290561
rosa_laevigata RLG00000012193 RLG00000012197
rosa_multiflora Rmu_sc0017853.1_g000005 Rmu_sc0022570.1_g000001
rosa_roxburghii Rroxscaffold_7G00176890
rosa_rugosa Rorug06G0213100
rosa_samantha Rh6AG324200 Rh6DG324700
rosa_wichuraiana Rw6G028130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 38, 110
AccB1I GGYRCC 2 cut(s) 566, 992
AccII CGCG 3 cut(s) 44, 111, 534
AciI CCGC 2 cut(s) 353, 788
AclWI GGATC 3 cut(s) 390, 718, 872
AcsI RAATTY 5 cut(s) 76, 359, 479, 605, 1250
AfaI GTAC 5 cut(s) 200, 286, 823, 843, 1232
AfiI CCNNNNNNNGG 4 cut(s) 79, 127, 563, 1027
AflIII ACRYGT 1 cut(s) 532
AgsI TTSAA 5 cut(s) 526, 681, 1016, 1079, 1195
AhlI ACTAGT 1 cut(s) 956
AjnI CCWGG 3 cut(s) 474, 948, 1111
AluBI AGCT 3 cut(s) 217, 437, 1184
AluI AGCT 3 cut(s) 217, 437, 1184
Alw26I GTCTC 4 cut(s) 145, 171, 667, 1100
AlwI GGATC 3 cut(s) 390, 718, 872
AlwNI CAGNNNCTG 1 cut(s) 1184
AoxI GGCC 1 cut(s) 1082
ApeKI GCWGC 3 cut(s) 276, 434, 1181
ApoI RAATTY 5 cut(s) 76, 359, 479, 605, 1250
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AspLEI GCGC 3 cut(s) 46, 276, 592
AspS9I GGNCC 3 cut(s) 103, 749, 1082
AsuHPI GGTGA 2 cut(s) 245, 686
AvaII GGWCC 2 cut(s) 103, 749
AxyI CCTNAGG 2 cut(s) 706, 967
BaeGI GKGCMC 1 cut(s) 571
BaeI ACNNNNGTAYC 2 cut(s) 945, 978
BanI GGYRCC 2 cut(s) 566, 992
BauI CACGAG 1 cut(s) 1032
BbsI GAAGAC 1 cut(s) 106
BbvCI CCTCAGC 1 cut(s) 1185
BbvI GCAGC 3 cut(s) 263, 446, 1193
BccI CCATC 5 cut(s) 326, 398, 485, 557, 976
BceAI ACGGC 1 cut(s) 818
BcgI CGANNNNNNTGC 4 cut(s) 81, 115, 866, 900
BciT130I CCWGG 3 cut(s) 476, 950, 1113
BciVI GTATCC 1 cut(s) 973
BcoDI GTCTC 4 cut(s) 145, 171, 667, 1100
BcuI ACTAGT 1 cut(s) 956
BfaI CTAG 1 cut(s) 957
BfmI CTRYAG 1 cut(s) 312
BfoI RGCGCY 1 cut(s) 277
BfuI GTATCC 1 cut(s) 973
BisI GCNGC 4 cut(s) 277, 435, 789, 1182
BlsI GCNGC 4 cut(s) 278, 436, 790, 1183
BmcAI AGTACT 2 cut(s) 286, 1232
Bme1390I CCNGG 3 cut(s) 476, 950, 1113
Bme18I GGWCC 2 cut(s) 103, 749
BmgT120I GGNCC 3 cut(s) 103, 749, 1082
BmiI GGNNCC 3 cut(s) 337, 568, 994
BmrFI CCNGG 3 cut(s) 476, 950, 1113
BmsI GCATC 1 cut(s) 863
BpiI GAAGAC 1 cut(s) 106
Bpu10I CCTNAGC 1 cut(s) 1185
BsaI GGTCTC 2 cut(s) 171, 1100
BsaJI CCNNGG 4 cut(s) 649, 906, 1111, 1112
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 127, 563, 1027
Bse21I CCTNAGG 2 cut(s) 706, 967
BseBI CCWGG 3 cut(s) 476, 950, 1113
BseDI CCNNGG 4 cut(s) 649, 906, 1111, 1112
BseGI GGATG 3 cut(s) 157, 394, 987
BseLI CCNNNNNNNGG 4 cut(s) 79, 127, 563, 1027
BseMII CTCAG 5 cut(s) 309, 650, 720, 981, 1176
BseRI GAGGAG 1 cut(s) 791
BseSI GKGCMC 1 cut(s) 571
BseXI GCAGC 3 cut(s) 263, 446, 1193
BsgI GTGCAG 2 cut(s) 33, 156
Bsh1236I CGCG 3 cut(s) 44, 111, 534
BshFI GGCC 1 cut(s) 1084
BshNI GGYRCC 2 cut(s) 566, 992
BslI CCNNNNNNNGG 4 cut(s) 79, 127, 563, 1027
BsmAI GTCTC 4 cut(s) 145, 171, 667, 1100
BsnI GGCC 1 cut(s) 1084
Bso31I GGTCTC 2 cut(s) 171, 1100
Bsp1286I GDGCHC 1 cut(s) 571
Bsp1407I TGTACA 1 cut(s) 841
Bsp143I GATC 4 cut(s) 382, 514, 710, 877
BspACI CCGC 2 cut(s) 353, 788
BspANI GGCC 1 cut(s) 1084
BspCNI CTCAG 5 cut(s) 308, 649, 719, 980, 1177
BspFNI CGCG 3 cut(s) 44, 111, 534
BspLI GGNNCC 3 cut(s) 337, 568, 994
BspMAI CTGCAG 1 cut(s) 316
BspPI GGATC 3 cut(s) 390, 718, 872
BspT107I GGYRCC 2 cut(s) 566, 992
BspTNI GGTCTC 2 cut(s) 171, 1100
BsrGI TGTACA 1 cut(s) 841
BssECI CCNNGG 4 cut(s) 649, 906, 1111, 1112
BssMI GATC 4 cut(s) 382, 514, 710, 877
BssSI CACGAG 1 cut(s) 1032
BssT1I CCWWGG 2 cut(s) 649, 906
Bst2BI CACGAG 1 cut(s) 1032
Bst2UI CCWGG 3 cut(s) 476, 950, 1113
Bst4CI ACNGT 5 cut(s) 289, 685, 975, 1090, 1290
Bst6I CTCTTC 3 cut(s) 174, 768, 1141
BstAUI TGTACA 1 cut(s) 841
BstC8I GCNNGC 2 cut(s) 25, 48
BstDEI CTNAG 5 cut(s) 295, 636, 706, 967, 1185
BstF5I GGATG 3 cut(s) 157, 394, 987
BstFNI CGCG 3 cut(s) 44, 111, 534
BstH2I RGCGCY 1 cut(s) 277
BstHHI GCGC 3 cut(s) 46, 276, 592
BstKTI GATC 4 cut(s) 385, 517, 713, 880
BstMAI GTCTC 4 cut(s) 145, 171, 667, 1100
BstMBI GATC 4 cut(s) 382, 514, 710, 877
BstMWI GCNNNNNNNGC 2 cut(s) 56, 1187
BstNI CCWGG 3 cut(s) 476, 950, 1113
BstSCI CCNGG 3 cut(s) 474, 948, 1111
BstSFI CTRYAG 1 cut(s) 312
BstSLI GKGCMC 1 cut(s) 571
BstUI CGCG 3 cut(s) 44, 111, 534
BstV1I GCAGC 3 cut(s) 263, 446, 1193
BstV2I GAAGAC 1 cut(s) 106
BstX2I RGATCY 2 cut(s) 382, 710
BstYI RGATCY 2 cut(s) 382, 710
Bsu36I CCTNAGG 2 cut(s) 706, 967
BsuI GTATCC 1 cut(s) 973
BsuRI GGCC 1 cut(s) 1084
BtsCI GGATG 3 cut(s) 157, 394, 987
BtsIMutI CAGTG 1 cut(s) 514
Cac8I GCNNGC 2 cut(s) 25, 48
CaiI CAGNNNCTG 1 cut(s) 1184
CfoI GCGC 3 cut(s) 46, 276, 592
Cfr13I GGNCC 3 cut(s) 103, 749, 1082
CseI GACGC 1 cut(s) 100
Csp6I GTAC 5 cut(s) 199, 285, 822, 842, 1231
CviAII CATG 2 cut(s) 572, 1204
CviQI GTAC 5 cut(s) 199, 285, 822, 842, 1231
DdeI CTNAG 5 cut(s) 295, 636, 706, 967, 1185
DpnI GATC 4 cut(s) 384, 516, 712, 879
DpnII GATC 4 cut(s) 382, 514, 710, 877
DraI TTTAAA 1 cut(s) 763
DrdI GACNNNNNNGTC 2 cut(s) 38, 110
DseDI GACNNNNNNGTC 2 cut(s) 38, 110
Eam1104I CTCTTC 3 cut(s) 174, 768, 1141
EarI CTCTTC 3 cut(s) 174, 768, 1141
Eco130I CCWWGG 2 cut(s) 649, 906
Eco31I GGTCTC 2 cut(s) 171, 1100
Eco47I GGWCC 2 cut(s) 103, 749
Eco81I CCTNAGG 2 cut(s) 706, 967
EcoO109I RGGNCCY 1 cut(s) 749
EcoRI GAATTC 1 cut(s) 76
EcoRII CCWGG 3 cut(s) 474, 948, 1111
EcoT14I CCWWGG 2 cut(s) 649, 906
ErhI CCWWGG 2 cut(s) 649, 906
FaeI CATG 2 cut(s) 575, 1207
FalI AAGNNNNNCTT 4 cut(s) 642, 674, 1009, 1041
FatI CATG 2 cut(s) 571, 1203
Fnu4HI GCNGC 4 cut(s) 277, 435, 789, 1182
FokI GGATG 3 cut(s) 164, 401, 994
Fsp4HI GCNGC 4 cut(s) 277, 435, 789, 1182
FspBI CTAG 1 cut(s) 957
GlaI GCGC 3 cut(s) 45, 275, 591
GluI GCNGC 4 cut(s) 277, 435, 789, 1182
HaeII RGCGCY 1 cut(s) 277
HaeIII GGCC 1 cut(s) 1084
HgaI GACGC 1 cut(s) 100
HhaI GCGC 3 cut(s) 46, 276, 592
Hin1II CATG 2 cut(s) 575, 1207
Hin6I GCGC 3 cut(s) 44, 274, 590
HinP1I GCGC 3 cut(s) 44, 274, 590
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 5 cut(s) 155, 179, 266, 677, 779
HphI GGTGA 2 cut(s) 245, 686
Hpy166II GTNNAC 3 cut(s) 64, 261, 824
Hpy188I TCNGA 3 cut(s) 103, 298, 1051
Hpy8I GTNNAC 3 cut(s) 64, 261, 824
Hpy99I CGWCG 1 cut(s) 839
HpyAV CCTTC 3 cut(s) 116, 891, 1073
HpyCH4III ACNGT 5 cut(s) 289, 685, 975, 1090, 1290
HpyCH4V TGCA 7 cut(s) 50, 137, 279, 314, 633, 807, 853
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 1187
HpyF3I CTNAG 5 cut(s) 295, 636, 706, 967, 1185
Hsp92II CATG 2 cut(s) 575, 1207
HspAI GCGC 3 cut(s) 44, 274, 590
Kzo9I GATC 4 cut(s) 382, 514, 710, 877
LmnI GCTCC 2 cut(s) 203, 442
Lsp1109I GCAGC 3 cut(s) 263, 446, 1193
LweI GCATC 1 cut(s) 863
MaeI CTAG 1 cut(s) 957
MaeIII GTNAC 4 cut(s) 307, 793, 913, 985
MalI GATC 4 cut(s) 384, 516, 712, 879
MboI GATC 4 cut(s) 382, 514, 710, 877
MboII GAAGA 6 cut(s) 29, 106, 161, 785, 1109, 1158
MflI RGATCY 2 cut(s) 382, 710
MhlI GDGCHC 1 cut(s) 571
MluCI AATT 8 cut(s) 76, 359, 479, 605, 1124, 1136, 1211, 1250
MluI ACGCGT 1 cut(s) 532
MlyI GAGTC 1 cut(s) 788
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 9 cut(s) 31, 388, 715, 769, 976, 1006, 1033, 1180, 1256
MseI TTAA 3 cut(s) 537, 762, 1056
MspA1I CMGCKG 1 cut(s) 1184
MspR9I CCNGG 3 cut(s) 476, 950, 1113
MvaI CCWGG 3 cut(s) 476, 950, 1113
MvnI CGCG 3 cut(s) 44, 111, 534
MwoI GCNNNNNNNGC 2 cut(s) 56, 1187
NdeII GATC 4 cut(s) 382, 514, 710, 877
NlaIII CATG 2 cut(s) 575, 1207
NlaIV GGNNCC 3 cut(s) 337, 568, 994
NmuCI GTSAC 1 cut(s) 307
PasI CCCWGGG 1 cut(s) 1112
PfeI GAWTC 4 cut(s) 155, 179, 266, 677
PkrI GCNGC 4 cut(s) 278, 436, 790, 1183
PleI GAGTC 1 cut(s) 787
PpsI GAGTC 1 cut(s) 787
PpuMI RGGWCCY 1 cut(s) 749
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 3 cut(s) 474, 948, 1111
PspGI CCWGG 3 cut(s) 474, 948, 1111
PspN4I GGNNCC 3 cut(s) 337, 568, 994
PspPI GGNCC 3 cut(s) 103, 749, 1082
PspPPI RGGWCCY 1 cut(s) 749
PstI CTGCAG 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 1184
PsuI RGATCY 2 cut(s) 382, 710
PvuII CAGCTG 1 cut(s) 1184
RsaI GTAC 5 cut(s) 200, 286, 823, 843, 1232
RsaNI GTAC 5 cut(s) 199, 285, 822, 842, 1231
SaqAI TTAA 3 cut(s) 537, 762, 1056
SatI GCNGC 4 cut(s) 277, 435, 789, 1182
Sau3AI GATC 4 cut(s) 382, 514, 710, 877
Sau96I GGNCC 3 cut(s) 103, 749, 1082
ScaI AGTACT 2 cut(s) 286, 1232
SchI GAGTC 1 cut(s) 788
ScrFI CCNGG 3 cut(s) 476, 950, 1113
SduI GDGCHC 1 cut(s) 571
SfaNI GCATC 1 cut(s) 863
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 2 cut(s) 103, 749
SpeI ACTAGT 1 cut(s) 956
Sse9I AATT 8 cut(s) 76, 359, 479, 605, 1124, 1136, 1211, 1250
SsiI CCGC 2 cut(s) 353, 788
SspMI CTAG 1 cut(s) 957
StyD4I CCNGG 3 cut(s) 474, 948, 1111
StyI CCWWGG 2 cut(s) 649, 906
TaaI ACNGT 5 cut(s) 289, 685, 975, 1090, 1290
TaqI TCGA 3 cut(s) 117, 897, 1131
TasI AATT 8 cut(s) 76, 359, 479, 605, 1124, 1136, 1211, 1250
TatI WGTACW 4 cut(s) 284, 821, 841, 1230
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 4 cut(s) 155, 179, 266, 677
Tru1I TTAA 3 cut(s) 537, 762, 1056
Tru9I TTAA 3 cut(s) 537, 762, 1056
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 3 cut(s) 276, 434, 1181
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 161
TspGWI ACGGA 2 cut(s) 50, 216
TspRI CASTG 1 cut(s) 514
VpaK11BI GGWCC 2 cut(s) 103, 749
XapI RAATTY 5 cut(s) 76, 359, 479, 605, 1250
XspI CTAG 1 cut(s) 957
ZrmI AGTACT 2 cut(s) 286, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.