Rroxscaffold_7G00176890

Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014488
Physical Location & Seq
Reverse (-)
16532843 .. 16537131
4289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00176890.1

Sequence Viewer

Length: 1287 bp
ATGGAGGACACATACGAAGAAACCAACAAGGACACGGAGGTCGCTCCTGCACTCATCGCAGTCCACCCGACCCAGAATTCGGTTGCAGTGGCAATCGGGTCGGATCTTCGCGTCTTCAACCTTATTGGGGGTTGTGCGGTGTCTTTGGTGGAAAATTCTGGTGGGGCGCTTCATAAAGATTCCATAAGAGCCATTCGGTACGGAGCAAGTGGGAAGTTATTTGTGTCTGCTGGTGATGATAAACTTGTCAAGATATGGTCAACTGAATCTTGGCATTGCATTAGTACTGTGTGCTCAGAGAAAAGAGTGACTGCAGTTGCCATAAGCAACGATGGTTCCTATGTTTGTTTTGCTGACAAATTTGGGGTTGTTTGGGTTGTGGATGTGGATGTGGAGGGCTTTGATGGAAACCAAGCGTCTGCTAACAAAAAGGCAGCACCTATGCTTTCTCACTATTGTAGCATCATAACAAGCCTGGAGTTTTCTCCTGATGGACGATTTTTTGTCAGTGCTGATCGGGATTTCAAAATTCGTGTTACTGTGTTTCCCAAGAAGCCTCTAGATGGAGCCCATGAGATACAAAGTTTTGCACTTGGTCATACAGAATTTGTTTCCTGCCTTGCCTCGGTTTACACTCAGGAATTCCCCACGGTTTTCTTGTCTCTGGAAGTGGTTCGCCTATGGGATATTTCCTCAGGATCTCTTTTAGATACTTGTGATATTAGAGACAAGGCAGGACCTTCGGAGTTTAAAGGAATAGAAGAAGAGTGCTGTTCTGCAGTAACTGATTTATGCACCATCCCAGATAGTACACTTGTTGCAGTGGCTGTACAAAGTTTGCAAGGGATTATATTGTTGAGATGTGATCTTTCTGCTCGAACACTCAATGTTGCCAAGGTGGTATCTATCAAGGGAGATACATTTATTCCTACAAGCCTGGCAACTAGTGTATCCTCGGGATTATTGTGGATGGTAACAGGTGCCTCTAACTTGGGTGATCTTGAAAGTCCCTCTTTGGCTCGTGTCAGGGTTATCTCTGACTTTAAGAAAACCAGTCCTGATGTTGTTCAGCAAGGGCCAACTGTTTTGGAAGATGATGAGACCCCTGGGGGCGAGAAATTGCTCGAAAAATTGCAGGGAAGAGTGTCCATTGACGAAAAGGTTTTCTCGACAGCAGCTGAGGCTTTGAAAACATCCATGAGCAAATTACTAATAAAGAAGCAGTACTCTACAGAGAGAAGAGAATTTAGAAAGAGAGGTAGAAATGATAAGAAACTCAAACAGTGA

Protein Analysis

428

Amino Acids

46.45

Weight (kDa)

5.46

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 20 - 129 2.4e-06 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 24 - 209 5.7e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 35 - 184 6.1e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 57 - 130 1e-10 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 57 - 244 1.6e-09 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 58 - 239 1.9e-13 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 58 - 116 8.4e-08 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 70 - 184 5.9e-10 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03110
fragaria_vesca FvH4_2g23260
malus_domestica MD03G1261900.v1.1 MD11G1282600.v1.1
prunus_persica Prupe.8G238200_v2.0.a1
pyrus_communis pycom03g20960 pycom11g25030
rosa_chinensis RchiOBHm_Chr6g0290561
rosa_laevigata RLG00000012193 RLG00000012197
rosa_multiflora Rmu_sc0017853.1_g000005 Rmu_sc0022570.1_g000001
rosa_roxburghii Rroxscaffold_7G00176890
rosa_rugosa Rorug06G0213100
rosa_samantha Rh6AG324200 Rh6DG324700
rosa_wichuraiana Rw6G028130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AccB1I GGYRCC 1 cut(s) 980
AccII CGCG 1 cut(s) 111
AciI CCGC 1 cut(s) 137
AclWI GGATC 2 cut(s) 111, 706
AcsI RAATTY 7 cut(s) 76, 154, 359, 528, 605, 641, 1244
AfaI GTAC 5 cut(s) 200, 286, 811, 831, 1226
AfiI CCNNNNNNNGG 4 cut(s) 79, 127, 563, 625
AgsI TTSAA 4 cut(s) 118, 526, 1004, 1189
AhlI ACTAGT 1 cut(s) 944
AjnI CCWGG 3 cut(s) 474, 936, 1105
AluBI AGCT 1 cut(s) 1178
AluI AGCT 1 cut(s) 1178
Alw21I GWGCWC 1 cut(s) 296
Alw26I GTCTC 3 cut(s) 666, 720, 1094
AlwI GGATC 2 cut(s) 111, 706
AlwNI CAGNNNCTG 3 cut(s) 785, 827, 1178
Ama87I CYCGRG 1 cut(s) 955
AoxI GGCC 1 cut(s) 1076
ApeKI GCWGC 2 cut(s) 434, 1175
ApoI RAATTY 7 cut(s) 76, 154, 359, 528, 605, 641, 1244
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
Asp700I GAANNNNTTC 1 cut(s) 672
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 2 cut(s) 737, 1076
AsuHPI GGTGA 2 cut(s) 245, 1007
AvaI CYCGRG 1 cut(s) 955
AvaII GGWCC 1 cut(s) 737
AxyI CCTNAGG 1 cut(s) 694
BaeI ACNNNNGTAYC 2 cut(s) 933, 966
BanI GGYRCC 1 cut(s) 980
BanII GRGCYC 1 cut(s) 571
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 296
BbvCI CCTCAGC 1 cut(s) 1179
BbvI GCAGC 2 cut(s) 446, 1187
BccI CCATC 6 cut(s) 326, 398, 485, 557, 806, 964
BcgI CGANNNNNNTGC 4 cut(s) 81, 115, 723, 757
BciT130I CCWGG 3 cut(s) 476, 938, 1107
BciVI GTATCC 1 cut(s) 961
BcoDI GTCTC 3 cut(s) 666, 720, 1094
BcuI ACTAGT 1 cut(s) 944
BfaI CTAG 2 cut(s) 560, 945
BfmI CTRYAG 3 cut(s) 312, 777, 1230
BfoI RGCGCY 1 cut(s) 170
BfuI GTATCC 1 cut(s) 961
BisI GCNGC 2 cut(s) 435, 1176
BlsI GCNGC 2 cut(s) 436, 1177
BmcAI AGTACT 2 cut(s) 286, 1226
Bme1390I CCNGG 3 cut(s) 476, 938, 1107
Bme18I GGWCC 1 cut(s) 737
BmeT110I CYCGRG 1 cut(s) 955
BmgT120I GGNCC 2 cut(s) 737, 1076
BmiI GGNNCC 3 cut(s) 337, 568, 982
BmrFI CCNGG 3 cut(s) 476, 938, 1107
BmsI GCATC 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 106
BpmI CTGGAG 1 cut(s) 497
Bpu10I CCTNAGC 1 cut(s) 1179
BsaI GGTCTC 1 cut(s) 1094
BsaJI CCNNGG 6 cut(s) 624, 648, 894, 954, 1105, 1106
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 127, 563, 625
Bse1I ACTGG 1 cut(s) 1053
Bse21I CCTNAGG 1 cut(s) 694
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 3 cut(s) 476, 938, 1107
BseDI CCNNGG 6 cut(s) 624, 648, 894, 954, 1105, 1106
BseGI GGATG 5 cut(s) 388, 394, 798, 975, 1193
BseLI CCNNNNNNNGG 4 cut(s) 79, 127, 563, 625
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 4 cut(s) 309, 650, 708, 1170
BseNI ACTGG 1 cut(s) 1053
BseXI GCAGC 2 cut(s) 446, 1187
BsgI GTGCAG 1 cut(s) 33
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 1 cut(s) 1078
BshNI GGYRCC 1 cut(s) 980
BsiHKAI GWGCWC 1 cut(s) 296
BsiHKCI CYCGRG 1 cut(s) 955
BslFI GGGAC 1 cut(s) 993
BslI CCNNNNNNNGG 4 cut(s) 79, 127, 563, 625
BsmAI GTCTC 3 cut(s) 666, 720, 1094
BsmFI GGGAC 1 cut(s) 993
BsnI GGCC 1 cut(s) 1078
Bso31I GGTCTC 1 cut(s) 1094
BsoBI CYCGRG 1 cut(s) 955
Bsp1286I GDGCHC 2 cut(s) 296, 571
Bsp1407I TGTACA 1 cut(s) 829
Bsp143I GATC 5 cut(s) 103, 514, 698, 865, 997
BspACI CCGC 1 cut(s) 137
BspANI GGCC 1 cut(s) 1078
BspCNI CTCAG 4 cut(s) 308, 649, 707, 1171
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 3 cut(s) 337, 568, 982
BspMAI CTGCAG 2 cut(s) 316, 781
BspPI GGATC 2 cut(s) 111, 706
BspT107I GGYRCC 1 cut(s) 980
BspTNI GGTCTC 1 cut(s) 1094
BsrDI GCAATG 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 829
BsrI ACTGG 1 cut(s) 1053
BssECI CCNNGG 6 cut(s) 624, 648, 894, 954, 1105, 1106
BssMI GATC 5 cut(s) 103, 514, 698, 865, 997
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 1 cut(s) 894
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 3 cut(s) 476, 938, 1107
Bst4CI ACNGT 5 cut(s) 289, 541, 652, 1084, 1284
Bst6I CTCTTC 3 cut(s) 759, 1135, 1234
BstAUI TGTACA 1 cut(s) 829
BstDEI CTNAG 4 cut(s) 295, 636, 694, 1179
BstDSI CCRYGG 1 cut(s) 648
BstF5I GGATG 5 cut(s) 388, 394, 798, 975, 1193
BstFNI CGCG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 5 cut(s) 106, 517, 701, 868, 1000
BstMAI GTCTC 3 cut(s) 666, 720, 1094
BstMBI GATC 5 cut(s) 103, 514, 698, 865, 997
BstMWI GCNNNNNNNGC 2 cut(s) 56, 1181
BstNI CCWGG 3 cut(s) 476, 938, 1107
BstSCI CCNGG 3 cut(s) 474, 936, 1105
BstSFI CTRYAG 3 cut(s) 312, 777, 1230
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 2 cut(s) 446, 1187
BstV2I GAAGAC 1 cut(s) 106
BstX2I RGATCY 2 cut(s) 103, 698
BstYI RGATCY 2 cut(s) 103, 698
Bsu36I CCTNAGG 1 cut(s) 694
BsuI GTATCC 1 cut(s) 961
BsuRI GGCC 1 cut(s) 1078
BtgI CCRYGG 1 cut(s) 648
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 5 cut(s) 388, 394, 798, 975, 1193
BtsI GCAGTG 2 cut(s) 93, 828
BtsIMutI CAGTG 3 cut(s) 93, 514, 828
CaiI CAGNNNCTG 3 cut(s) 785, 827, 1178
CfoI GCGC 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 737, 1076
CseI GACGC 2 cut(s) 100, 405
Csp6I GTAC 5 cut(s) 199, 285, 810, 830, 1225
CviAII CATG 2 cut(s) 572, 1198
CviQI GTAC 5 cut(s) 199, 285, 810, 830, 1225
DdeI CTNAG 4 cut(s) 295, 636, 694, 1179
DpnI GATC 5 cut(s) 105, 516, 700, 867, 999
DpnII GATC 5 cut(s) 103, 514, 698, 865, 997
DraI TTTAAA 1 cut(s) 751
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
Eam1104I CTCTTC 3 cut(s) 759, 1135, 1234
EarI CTCTTC 3 cut(s) 759, 1135, 1234
Eco130I CCWWGG 1 cut(s) 894
Eco24I GRGCYC 1 cut(s) 571
Eco31I GGTCTC 1 cut(s) 1094
Eco47I GGWCC 1 cut(s) 737
Eco81I CCTNAGG 1 cut(s) 694
Eco88I CYCGRG 1 cut(s) 955
EcoO109I RGGNCCY 1 cut(s) 737
EcoRI GAATTC 2 cut(s) 76, 641
EcoRII CCWGG 3 cut(s) 474, 936, 1105
EcoT14I CCWWGG 1 cut(s) 894
EcoT38I GRGCYC 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 894
FaeI CATG 2 cut(s) 575, 1201
FalI AAGNNNNNCTT 2 cut(s) 997, 1029
FaqI GGGAC 1 cut(s) 993
FatI CATG 2 cut(s) 571, 1197
Fnu4HI GCNGC 2 cut(s) 435, 1176
FokI GGATG 5 cut(s) 395, 401, 785, 982, 1180
FriOI GRGCYC 1 cut(s) 571
Fsp4HI GCNGC 2 cut(s) 435, 1176
FspBI CTAG 2 cut(s) 560, 945
GlaI GCGC 1 cut(s) 168
GluI GCNGC 2 cut(s) 435, 1176
GsuI CTGGAG 1 cut(s) 497
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 1 cut(s) 1078
HgaI GACGC 2 cut(s) 100, 405
HhaI GCGC 1 cut(s) 169
Hin1II CATG 2 cut(s) 575, 1201
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 2 cut(s) 179, 266
HphI GGTGA 2 cut(s) 245, 1007
Hpy166II GTNNAC 4 cut(s) 64, 261, 631, 812
Hpy188I TCNGA 4 cut(s) 103, 298, 745, 1039
Hpy8I GTNNAC 4 cut(s) 64, 261, 631, 812
HpyAV CCTTC 1 cut(s) 750
HpyCH4III ACNGT 5 cut(s) 289, 541, 652, 1084, 1284
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 1181
HpyF3I CTNAG 4 cut(s) 295, 636, 694, 1179
Hsp92II CATG 2 cut(s) 575, 1201
HspAI GCGC 1 cut(s) 167
Kzo9I GATC 5 cut(s) 103, 514, 698, 865, 997
LmnI GCTCC 3 cut(s) 49, 203, 566
Lsp1109I GCAGC 2 cut(s) 446, 1187
LweI GCATC 1 cut(s) 471
MaeI CTAG 2 cut(s) 560, 945
MaeIII GTNAC 4 cut(s) 307, 535, 781, 973
MalI GATC 5 cut(s) 105, 516, 700, 867, 999
MboI GATC 5 cut(s) 103, 514, 698, 865, 997
MboII GAAGA 8 cut(s) 29, 98, 106, 773, 776, 1103, 1152, 1251
MflI RGATCY 2 cut(s) 103, 698
MhlI GDGCHC 2 cut(s) 296, 571
MmeI TCCRAC 1 cut(s) 81
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 2 cut(s) 750, 1044
MspA1I CMGCKG 1 cut(s) 1178
MspR9I CCNGG 3 cut(s) 476, 938, 1107
MvaI CCWGG 3 cut(s) 476, 938, 1107
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 56, 1181
NdeII GATC 5 cut(s) 103, 514, 698, 865, 997
NlaIII CATG 2 cut(s) 575, 1201
NlaIV GGNNCC 3 cut(s) 337, 568, 982
NmuCI GTSAC 1 cut(s) 307
PasI CCCWGGG 1 cut(s) 1106
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 2 cut(s) 179, 266
PkrI GCNGC 2 cut(s) 436, 1177
PpuMI RGGWCCY 1 cut(s) 737
Psp5II RGGWCCY 1 cut(s) 737
Psp6I CCWGG 3 cut(s) 474, 936, 1105
PspGI CCWGG 3 cut(s) 474, 936, 1105
PspN4I GGNNCC 3 cut(s) 337, 568, 982
PspPI GGNCC 2 cut(s) 737, 1076
PspPPI RGGWCCY 1 cut(s) 737
PstI CTGCAG 2 cut(s) 316, 781
PstNI CAGNNNCTG 3 cut(s) 785, 827, 1178
PsuI RGATCY 2 cut(s) 103, 698
PvuII CAGCTG 1 cut(s) 1178
RsaI GTAC 5 cut(s) 200, 286, 811, 831, 1226
RsaNI GTAC 5 cut(s) 199, 285, 810, 830, 1225
SaqAI TTAA 2 cut(s) 750, 1044
SatI GCNGC 2 cut(s) 435, 1176
Sau3AI GATC 5 cut(s) 103, 514, 698, 865, 997
Sau96I GGNCC 2 cut(s) 737, 1076
ScaI AGTACT 2 cut(s) 286, 1226
ScrFI CCNGG 3 cut(s) 476, 938, 1107
SduI GDGCHC 2 cut(s) 296, 571
SetI ASST 9 cut(s) 42, 123, 442, 742, 900, 982, 1164, 1180, 1261
SfaNI GCATC 1 cut(s) 471
SfcI CTRYAG 3 cut(s) 312, 777, 1230
SinI GGWCC 1 cut(s) 737
SpeI ACTAGT 1 cut(s) 944
SsiI CCGC 1 cut(s) 137
SspMI CTAG 2 cut(s) 560, 945
StyD4I CCNGG 3 cut(s) 474, 936, 1105
StyI CCWWGG 1 cut(s) 894
TaaI ACNGT 5 cut(s) 289, 541, 652, 1084, 1284
TaqI TCGA 3 cut(s) 877, 1125, 1169
TatI WGTACW 4 cut(s) 284, 809, 829, 1224
TfiI GAWTC 2 cut(s) 179, 266
Tru1I TTAA 2 cut(s) 750, 1044
Tru9I TTAA 2 cut(s) 750, 1044
TscAI CASTG 3 cut(s) 93, 514, 828
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 2 cut(s) 434, 1175
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 161
TspGWI ACGGA 2 cut(s) 50, 216
TspRI CASTG 3 cut(s) 93, 514, 828
VpaK11BI GGWCC 1 cut(s) 737
XapI RAATTY 7 cut(s) 76, 154, 359, 528, 605, 641, 1244
XbaI TCTAGA 1 cut(s) 559
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 2 cut(s) 560, 945
ZrmI AGTACT 2 cut(s) 286, 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.