RLG00000012193

Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014488
Physical Location & Seq
Reverse (-)
16160090 .. 16164044
3955 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012193

Sequence Viewer

Length: 1299 bp
ATGGAGGACACATACGAAGAAACCAACAAGGACACGGAGGTCGCTCCGGCCCTCATCGCAGTCCACCCGACCCAGAATTCGGTTGCACTCGCAATCGGGTCGGACCTTCGCGTCTTCAACCTTATTGTGGGTTGTGCGGTGTCTTTGGTGGAAAATTCTGGTGGGGCACTTCATAAAGATTCCATAAGAGCCATTCGGTATGGAGCAAGTGGGAAGTTATTTGTGTCTGCTGGTGACGATAAACTTGTCAAGATATGGTCAACTGAATCTTGGCACTGCATTAGTACTGTGTGCTCAGAGAAAAGAGTGACTGCAGTTGCCATAAGCAACGATGGTTCCTATGTTTGTTTTGCTGACAAATTTGGGGTTGTTTGGGTTGTGGATGTGGATGTGGAGGGCTTTGATGGAAACCAAGTGTCTGCTAACAAAAAGGCAGCACCTATGCTTTCTCACTATTGTAGCATCATAACAAGCCTGGAGTTTTCTCCTGATGGACGATTTTTTGTCAGTGCTGATCGGGATTTCAAAATTCGTGTTACTGTGTTTCCCAAGAAGCCTCTAGATGGAGCCCATGAGATACAAAGTTTTGCACTTGGTCATACAGAATTTGTTTCCTGCCTTGCCTCTGTTTACACTCAGGAATTCCCCCACGGTTTTCTTGTCTCTGGAAGTGGTGATTCAACTGTTCGCCTATGGGATATTTCCTCAGGATCTCTTTTAGATACTTGTGATATTAGAGACAAGGCAGGACCTTCGGAGTTTAAAGGAATAGAAGAAGAGTGCTGTTCTGCAGTAACTGATTTATGCACCATCCCAGATTGTACACTTGTTGCAGTGGCTGTACAAAGTTTGCAAGGGATTATATTGTTGAGATGTGATCTTTCTGCTCGAACACTCAATGTTGCCAAGGTGGTATCTATCAAGGGAGATACATTTATTCCTACAAGCCTGGCAACTAGTGTATCCTCGGGATTATTGTGGATGGTAACAGGTGCCTCTAACTTGGGTGATCTTGAAAGCACCTCTTTGGCTCGTGTCAGGGTTATCTCTGACTTTAAGAAAACCAGTCCTGATGTTGTTCAGCAAGGGCCAACTGTTTTGGAAGATGATGAGACCCCTGGGGGGGAGAAATTGCTTGAAAAATTGCAGGGAAGAGTGTCCATTGACGAAAAGGTTTTCTCGACAGCAGCTGAGGCTTTGAAAACATCCATGAGCAAATTACTAATAAAGAAGCAGTACTCTACAGAGAGAAGAGAATTTAGAAAGAGAGGTAGAAATGATAAGAAACTCAAACAGTGA

Protein Analysis

433

Amino Acids

46.81

Weight (kDa)

5.54

Isoelectric Point (pI)

30.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 19 - 179 3.2e-09 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 24 - 246 5.9e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 36 - 184 1.2e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 57 - 130 7.6e-11 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 57 - 247 4e-13 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 58 - 119 8e-08 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 58 - 116 8.8e-08 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 70 - 184 4.1e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 71 - 240 1.7e-09 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 101 - 242 1.4e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 149 - 243 1.8e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 156 - 245 6.6e-09 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03110
fragaria_vesca FvH4_2g23260
malus_domestica MD03G1261900.v1.1 MD11G1282600.v1.1
prunus_persica Prupe.8G238200_v2.0.a1
pyrus_communis pycom03g20960 pycom11g25030
rosa_chinensis RchiOBHm_Chr6g0290561
rosa_laevigata RLG00000012193 RLG00000012197
rosa_multiflora Rmu_sc0017853.1_g000005 Rmu_sc0022570.1_g000001
rosa_roxburghii Rroxscaffold_7G00176890
rosa_rugosa Rorug06G0213100
rosa_samantha Rh6AG324200 Rh6DG324700
rosa_wichuraiana Rw6G028130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 38, 110
AccB1I GGYRCC 1 cut(s) 992
AccII CGCG 1 cut(s) 111
AciI CCGC 1 cut(s) 137
AclWI GGATC 1 cut(s) 718
AcsI RAATTY 7 cut(s) 76, 154, 359, 528, 605, 641, 1256
AfaI GTAC 4 cut(s) 286, 823, 843, 1238
AfiI CCNNNNNNNGG 5 cut(s) 79, 127, 563, 1122, 1123
AgsI TTSAA 6 cut(s) 118, 526, 681, 1016, 1139, 1201
AhlI ACTAGT 1 cut(s) 956
AjnI CCWGG 3 cut(s) 474, 948, 1117
AluBI AGCT 1 cut(s) 1190
AluI AGCT 1 cut(s) 1190
Alw21I GWGCWC 1 cut(s) 296
Alw26I GTCTC 3 cut(s) 667, 732, 1106
AlwI GGATC 1 cut(s) 718
AlwNI CAGNNNCTG 3 cut(s) 797, 839, 1190
Ama87I CYCGRG 1 cut(s) 967
AoxI GGCC 2 cut(s) 48, 1088
ApeKI GCWGC 2 cut(s) 434, 1187
ApoI RAATTY 7 cut(s) 76, 154, 359, 528, 605, 641, 1256
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AspS9I GGNCC 4 cut(s) 49, 103, 749, 1088
AsuHPI GGTGA 3 cut(s) 245, 686, 1019
AvaI CYCGRG 1 cut(s) 967
AvaII GGWCC 2 cut(s) 103, 749
AxyI CCTNAGG 1 cut(s) 706
BaeGI GKGCMC 1 cut(s) 169
BaeI ACNNNNGTAYC 2 cut(s) 945, 978
BanI GGYRCC 1 cut(s) 992
BanII GRGCYC 1 cut(s) 571
BauI CACGAG 1 cut(s) 1032
BbsI GAAGAC 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 296
BbvCI CCTCAGC 1 cut(s) 1191
BbvI GCAGC 2 cut(s) 446, 1199
BccI CCATC 6 cut(s) 326, 398, 485, 557, 818, 976
BcgI CGANNNNNNTGC 4 cut(s) 81, 115, 735, 769
BciT130I CCWGG 3 cut(s) 476, 950, 1119
BciVI GTATCC 1 cut(s) 973
BcoDI GTCTC 3 cut(s) 667, 732, 1106
BcuI ACTAGT 1 cut(s) 956
BfaI CTAG 2 cut(s) 560, 957
BfmI CTRYAG 3 cut(s) 312, 789, 1242
BfuI GTATCC 1 cut(s) 973
BisI GCNGC 2 cut(s) 435, 1188
BlsI GCNGC 2 cut(s) 436, 1189
BmcAI AGTACT 2 cut(s) 286, 1238
Bme1390I CCNGG 3 cut(s) 476, 950, 1119
Bme18I GGWCC 2 cut(s) 103, 749
BmeT110I CYCGRG 1 cut(s) 967
BmgT120I GGNCC 4 cut(s) 49, 103, 749, 1088
BmiI GGNNCC 3 cut(s) 337, 568, 994
BmrFI CCNGG 3 cut(s) 476, 950, 1119
BmsI GCATC 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 106
BpmI CTGGAG 1 cut(s) 497
Bpu10I CCTNAGC 1 cut(s) 1191
BsaI GGTCTC 1 cut(s) 1106
BsaJI CCNNGG 5 cut(s) 649, 906, 966, 1117, 1118
Bsc4I CCNNNNNNNGG 5 cut(s) 79, 127, 563, 1122, 1123
Bse1I ACTGG 1 cut(s) 1065
Bse21I CCTNAGG 1 cut(s) 706
BseBI CCWGG 3 cut(s) 476, 950, 1119
BseDI CCNNGG 5 cut(s) 649, 906, 966, 1117, 1118
BseGI GGATG 5 cut(s) 388, 394, 810, 987, 1205
BseLI CCNNNNNNNGG 5 cut(s) 79, 127, 563, 1122, 1123
BseMII CTCAG 4 cut(s) 309, 650, 720, 1182
BseNI ACTGG 1 cut(s) 1065
BseSI GKGCMC 1 cut(s) 169
BseXI GCAGC 2 cut(s) 446, 1199
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 50, 1090
BshNI GGYRCC 1 cut(s) 992
BsiHKAI GWGCWC 1 cut(s) 296
BsiHKCI CYCGRG 1 cut(s) 967
BsiSI CCGG 1 cut(s) 47
BslI CCNNNNNNNGG 5 cut(s) 79, 127, 563, 1122, 1123
BsmAI GTCTC 3 cut(s) 667, 732, 1106
BsnI GGCC 2 cut(s) 50, 1090
Bso31I GGTCTC 1 cut(s) 1106
BsoBI CYCGRG 1 cut(s) 967
Bsp1286I GDGCHC 3 cut(s) 169, 296, 571
Bsp1407I TGTACA 2 cut(s) 821, 841
Bsp143I GATC 4 cut(s) 514, 710, 877, 1009
BspACI CCGC 1 cut(s) 137
BspANI GGCC 2 cut(s) 50, 1090
BspCNI CTCAG 4 cut(s) 308, 649, 719, 1183
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 3 cut(s) 337, 568, 994
BspMAI CTGCAG 2 cut(s) 316, 793
BspPI GGATC 1 cut(s) 718
BspT107I GGYRCC 1 cut(s) 992
BspTNI GGTCTC 1 cut(s) 1106
BsrGI TGTACA 2 cut(s) 821, 841
BsrI ACTGG 1 cut(s) 1065
BssECI CCNNGG 5 cut(s) 649, 906, 966, 1117, 1118
BssMI GATC 4 cut(s) 514, 710, 877, 1009
BssSI CACGAG 1 cut(s) 1032
BssT1I CCWWGG 1 cut(s) 906
Bst2BI CACGAG 1 cut(s) 1032
Bst2UI CCWGG 3 cut(s) 476, 950, 1119
Bst4CI ACNGT 6 cut(s) 289, 541, 653, 685, 1096, 1296
Bst6I CTCTTC 3 cut(s) 771, 1147, 1246
BstAUI TGTACA 2 cut(s) 821, 841
BstDEI CTNAG 4 cut(s) 295, 636, 706, 1191
BstDSI CCRYGG 1 cut(s) 649
BstF5I GGATG 5 cut(s) 388, 394, 810, 987, 1205
BstFNI CGCG 1 cut(s) 111
BstKTI GATC 4 cut(s) 517, 713, 880, 1012
BstMAI GTCTC 3 cut(s) 667, 732, 1106
BstMBI GATC 4 cut(s) 514, 710, 877, 1009
BstMWI GCNNNNNNNGC 2 cut(s) 56, 1193
BstNI CCWGG 3 cut(s) 476, 950, 1119
BstSCI CCNGG 3 cut(s) 474, 948, 1117
BstSFI CTRYAG 3 cut(s) 312, 789, 1242
BstSLI GKGCMC 1 cut(s) 169
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 2 cut(s) 446, 1199
BstV2I GAAGAC 1 cut(s) 106
BstX2I RGATCY 1 cut(s) 710
BstYI RGATCY 1 cut(s) 710
Bsu36I CCTNAGG 1 cut(s) 706
BsuI GTATCC 1 cut(s) 973
BsuRI GGCC 2 cut(s) 50, 1090
BtgI CCRYGG 1 cut(s) 649
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 5 cut(s) 388, 394, 810, 987, 1205
BtsI GCAGTG 2 cut(s) 274, 840
BtsIMutI CAGTG 3 cut(s) 274, 514, 840
CaiI CAGNNNCTG 3 cut(s) 797, 839, 1190
Cfr13I GGNCC 4 cut(s) 49, 103, 749, 1088
CseI GACGC 1 cut(s) 100
Csp6I GTAC 4 cut(s) 285, 822, 842, 1237
CviAII CATG 2 cut(s) 572, 1210
CviQI GTAC 4 cut(s) 285, 822, 842, 1237
DdeI CTNAG 4 cut(s) 295, 636, 706, 1191
DpnI GATC 4 cut(s) 516, 712, 879, 1011
DpnII GATC 4 cut(s) 514, 710, 877, 1009
DraI TTTAAA 1 cut(s) 763
DrdI GACNNNNNNGTC 2 cut(s) 38, 110
DseDI GACNNNNNNGTC 2 cut(s) 38, 110
Eam1104I CTCTTC 3 cut(s) 771, 1147, 1246
EarI CTCTTC 3 cut(s) 771, 1147, 1246
Eco130I CCWWGG 1 cut(s) 906
Eco24I GRGCYC 1 cut(s) 571
Eco31I GGTCTC 1 cut(s) 1106
Eco47I GGWCC 2 cut(s) 103, 749
Eco81I CCTNAGG 1 cut(s) 706
Eco88I CYCGRG 1 cut(s) 967
EcoO109I RGGNCCY 1 cut(s) 749
EcoRI GAATTC 2 cut(s) 76, 641
EcoRII CCWGG 3 cut(s) 474, 948, 1117
EcoT14I CCWWGG 1 cut(s) 906
EcoT38I GRGCYC 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 906
FaeI CATG 2 cut(s) 575, 1213
FalI AAGNNNNNCTT 2 cut(s) 1009, 1041
FatI CATG 2 cut(s) 571, 1209
Fnu4HI GCNGC 2 cut(s) 435, 1188
FokI GGATG 5 cut(s) 395, 401, 797, 994, 1192
FriOI GRGCYC 1 cut(s) 571
Fsp4HI GCNGC 2 cut(s) 435, 1188
FspBI CTAG 2 cut(s) 560, 957
GluI GCNGC 2 cut(s) 435, 1188
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 2 cut(s) 50, 1090
HapII CCGG 1 cut(s) 47
HgaI GACGC 1 cut(s) 100
Hin1II CATG 2 cut(s) 575, 1213
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 3 cut(s) 179, 266, 677
HpaII CCGG 1 cut(s) 47
HphI GGTGA 3 cut(s) 245, 686, 1019
Hpy166II GTNNAC 4 cut(s) 64, 261, 631, 824
Hpy188I TCNGA 4 cut(s) 103, 298, 757, 1051
Hpy8I GTNNAC 4 cut(s) 64, 261, 631, 824
HpyAV CCTTC 2 cut(s) 116, 762
HpyCH4III ACNGT 6 cut(s) 289, 541, 653, 685, 1096, 1296
HpyCH4V TGCA 9 cut(s) 86, 279, 314, 590, 791, 807, 833, 853, 1147
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 1193
HpyF3I CTNAG 4 cut(s) 295, 636, 706, 1191
Hsp92II CATG 2 cut(s) 575, 1213
Kzo9I GATC 4 cut(s) 514, 710, 877, 1009
LmnI GCTCC 3 cut(s) 49, 203, 566
Lsp1109I GCAGC 2 cut(s) 446, 1199
LweI GCATC 1 cut(s) 471
MaeI CTAG 2 cut(s) 560, 957
MaeIII GTNAC 5 cut(s) 233, 307, 535, 793, 985
MalI GATC 4 cut(s) 516, 712, 879, 1011
MboI GATC 4 cut(s) 514, 710, 877, 1009
MboII GAAGA 7 cut(s) 29, 106, 785, 788, 1115, 1164, 1263
MflI RGATCY 1 cut(s) 710
MhlI GDGCHC 3 cut(s) 169, 296, 571
MmeI TCCRAC 1 cut(s) 81
MseI TTAA 2 cut(s) 762, 1056
MspA1I CMGCKG 1 cut(s) 1190
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 3 cut(s) 476, 950, 1119
MvaI CCWGG 3 cut(s) 476, 950, 1119
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 56, 1193
NdeII GATC 4 cut(s) 514, 710, 877, 1009
NlaIII CATG 2 cut(s) 575, 1213
NlaIV GGNNCC 3 cut(s) 337, 568, 994
NmuCI GTSAC 2 cut(s) 233, 307
PasI CCCWGGG 1 cut(s) 1118
PfeI GAWTC 3 cut(s) 179, 266, 677
PkrI GCNGC 2 cut(s) 436, 1189
PpuMI RGGWCCY 1 cut(s) 749
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 3 cut(s) 474, 948, 1117
PspGI CCWGG 3 cut(s) 474, 948, 1117
PspN4I GGNNCC 3 cut(s) 337, 568, 994
PspPI GGNCC 4 cut(s) 49, 103, 749, 1088
PspPPI RGGWCCY 1 cut(s) 749
PstI CTGCAG 2 cut(s) 316, 793
PstNI CAGNNNCTG 3 cut(s) 797, 839, 1190
PsuI RGATCY 1 cut(s) 710
PvuII CAGCTG 1 cut(s) 1190
RsaI GTAC 4 cut(s) 286, 823, 843, 1238
RsaNI GTAC 4 cut(s) 285, 822, 842, 1237
SaqAI TTAA 2 cut(s) 762, 1056
SatI GCNGC 2 cut(s) 435, 1188
Sau3AI GATC 4 cut(s) 514, 710, 877, 1009
Sau96I GGNCC 4 cut(s) 49, 103, 749, 1088
ScaI AGTACT 2 cut(s) 286, 1238
ScrFI CCNGG 3 cut(s) 476, 950, 1119
SduI GDGCHC 3 cut(s) 169, 296, 571
SfaNI GCATC 1 cut(s) 471
SfcI CTRYAG 3 cut(s) 312, 789, 1242
SinI GGWCC 2 cut(s) 103, 749
SpeI ACTAGT 1 cut(s) 956
SsiI CCGC 1 cut(s) 137
SspMI CTAG 2 cut(s) 560, 957
StyD4I CCNGG 3 cut(s) 474, 948, 1117
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 6 cut(s) 289, 541, 653, 685, 1096, 1296
TaqI TCGA 2 cut(s) 889, 1181
TatI WGTACW 4 cut(s) 284, 821, 841, 1236
TfiI GAWTC 3 cut(s) 179, 266, 677
Tru1I TTAA 2 cut(s) 762, 1056
Tru9I TTAA 2 cut(s) 762, 1056
TscAI CASTG 3 cut(s) 281, 514, 840
TseFI GTSAC 2 cut(s) 233, 307
TseI GCWGC 2 cut(s) 434, 1187
Tsp45I GTSAC 2 cut(s) 233, 307
TspDTI ATGAA 1 cut(s) 161
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 3 cut(s) 281, 514, 840
VpaK11BI GGWCC 2 cut(s) 103, 749
XapI RAATTY 7 cut(s) 76, 154, 359, 528, 605, 641, 1256
XbaI TCTAGA 1 cut(s) 559
XspI CTAG 2 cut(s) 560, 957
ZrmI AGTACT 2 cut(s) 286, 1238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.