RLG00000012197

Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014488
Physical Location & Seq
Reverse (-)
16181352 .. 16185243
3892 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012197

Sequence Viewer

Length: 1143 bp
ATGGAGGACACATACGAAGAAACCAACAAGGACACGGAGGTCGCTCCGGCCCTCATCGCAGTCCACCCGACCCAGAATTCGGTTGCACTCGCAATCGGGTCGGACCTTCGCGTCTTCAACCTTATTGGGGGTTGTGCGGTGTCTTTGGTGGAAAATTCTGGTGGGGCACTTCATAAAGATTCCATAAGAGCCATTCGGTACAGAGCAAGTGGGAAGTTATTTGTGTCTGCTGGTGATGATAAACTTGTCAAGATATGGTCAACTGAATCTTGGCACTGCATTAGTACTGTCTGCTCAGAGAAAAGAGTGACTTCAGTTGCCATAAGCAACGATGGTTCCTATGTTTGTTTTGCTGACAAATTTGGGGTTGTTTGGGTTGTGGATGTGGATGTAGAGGGCTTTGACGGAAACCAAGTGTCTGCTGACAAAAAGGCAGCACCTATGCTTTCTCACTATTGTAGCATCATCACAAGCCTGGAGTTTTCTCCTGATGGACGATTTTTTGTCAGTGCTGATCGGGATTTCAAAATTCATGTTAATGTGTTTCCCAAGAAGCCTTTAGATGGAGCCCATGATATACAAAGTTTGGCGCTTGGTCATACAGAATTTGTTTCCTGCCTTGCCTTTGTTTGCACTCAGGAGTTCCCCCAGGGTTTTCTTGTCTCTGGAAGTGGTGATTCAACTGTTCGCCTATGGGATATTTCCTCAGGATCTCTTTTAGATACTTGTGATATTAGAGAAAAGGCAGGACCTTTAGAGTTTAAAGGAATAGAAGAGGAGTGCTGTGCAGCTGTTACTGATTTATGCACCGTCCCAGATAGTACACTTGTTGCAGTGGCTGTACAAAGTTTGCAAGGGAAGTTCTCTCATTATAGTTTGCAAGGGATTATATTGTTGAGATGTGATCTTTCTGCTCAAACACTAAATGTTGCCAAGGTGGTATCTATCAAGGGAGATACATTTATTCTACAAGCCTGGCAACCAGTGTATCCTCAGGACTATTGTGGATGTCCTGATGTTGTTCAGCAAGGGCCAACTGTTTTGGAAGATGATGAGATCCCTGGGGGCGAGAAATTGCTTGAAAATTTGCAGGGAAGAGTGTCAATTGATGAAAAGGTTTTCTCGCAGCGGCTGAGGCTTTGA

Protein Analysis

381

Amino Acids

41.27

Weight (kDa)

4.78

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 20 - 179 2.1e-09 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 24 - 260 9.2e-16 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 35 - 182 3.1e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 37 - 244 3.8e-23 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 57 - 249 1.5e-14 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 58 - 119 3e-08 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 58 - 116 2.8e-08 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 58 - 118 5e-06 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 68 - 209 5.2e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 71 - 240 1.1e-09 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 149 - 243 4.2e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 153 - 242 6.6e-09 WDR5 beta-propeller domain
WD40 PF00400 199 - 233 6.2e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03110
fragaria_vesca FvH4_2g23260
malus_domestica MD03G1261900.v1.1 MD11G1282600.v1.1
prunus_persica Prupe.8G238200_v2.0.a1
pyrus_communis pycom03g20960 pycom11g25030
rosa_chinensis RchiOBHm_Chr6g0290561
rosa_laevigata RLG00000012193 RLG00000012197
rosa_multiflora Rmu_sc0017853.1_g000005 Rmu_sc0022570.1_g000001
rosa_roxburghii Rroxscaffold_7G00176890
rosa_rugosa Rorug06G0213100
rosa_samantha Rh6AG324200 Rh6DG324700
rosa_wichuraiana Rw6G028130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 38, 110
AccII CGCG 1 cut(s) 111
AciI CCGC 2 cut(s) 137, 1129
AclWI GGATC 2 cut(s) 718, 1051
AcsI RAATTY 6 cut(s) 76, 154, 359, 528, 605, 1084
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 4 cut(s) 200, 286, 823, 843
AfiI CCNNNNNNNGG 3 cut(s) 79, 127, 563
AgsI TTSAA 4 cut(s) 118, 526, 681, 1082
AjnI CCWGG 4 cut(s) 474, 648, 974, 1060
AluBI AGCT 1 cut(s) 791
AluI AGCT 1 cut(s) 791
Alw26I GTCTC 1 cut(s) 667
AlwI GGATC 2 cut(s) 718, 1051
AlwNI CAGNNNCTG 2 cut(s) 839, 1132
AoxI GGCC 2 cut(s) 48, 1031
ApeKI GCWGC 3 cut(s) 434, 788, 1126
ApoI RAATTY 6 cut(s) 76, 154, 359, 528, 605, 1084
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AspLEI GCGC 1 cut(s) 592
AspS9I GGNCC 4 cut(s) 49, 103, 749, 1031
AsuHPI GGTGA 2 cut(s) 245, 686
AvaII GGWCC 2 cut(s) 103, 749
AxyI CCTNAGG 2 cut(s) 706, 993
BaeGI GKGCMC 1 cut(s) 169
BaeI ACNNNNGTAYC 2 cut(s) 971, 1004
BanII GRGCYC 1 cut(s) 571
BbsI GAAGAC 1 cut(s) 106
BbvCI CCTCAGC 1 cut(s) 1133
BbvI GCAGC 3 cut(s) 446, 800, 1138
BccI CCATC 3 cut(s) 326, 485, 557
BcgI CGANNNNNNTGC 2 cut(s) 81, 115
BciT130I CCWGG 4 cut(s) 476, 650, 976, 1062
BciVI GTATCC 1 cut(s) 999
BcoDI GTCTC 1 cut(s) 667
BfoI RGCGCY 1 cut(s) 593
BfuI GTATCC 1 cut(s) 999
BisI GCNGC 4 cut(s) 435, 789, 1127, 1130
BlsI GCNGC 4 cut(s) 436, 790, 1128, 1131
BmcAI AGTACT 1 cut(s) 286
Bme1390I CCNGG 4 cut(s) 476, 650, 976, 1062
Bme18I GGWCC 2 cut(s) 103, 749
BmgT120I GGNCC 4 cut(s) 49, 103, 749, 1031
BmiI GGNNCC 2 cut(s) 337, 568
BmrFI CCNGG 4 cut(s) 476, 650, 976, 1062
BmsI GCATC 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 106
BpmI CTGGAG 1 cut(s) 497
Bpu10I CCTNAGC 1 cut(s) 1133
BsaJI CCNNGG 5 cut(s) 648, 649, 933, 1060, 1061
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 127, 563
Bse1I ACTGG 1 cut(s) 983
Bse21I CCTNAGG 2 cut(s) 706, 993
BseBI CCWGG 4 cut(s) 476, 650, 976, 1062
BseDI CCNNGG 5 cut(s) 648, 649, 933, 1060, 1061
BseGI GGATG 3 cut(s) 388, 394, 1013
BseLI CCNNNNNNNGG 3 cut(s) 79, 127, 563
BseMII CTCAG 5 cut(s) 309, 650, 720, 1007, 1124
BseNI ACTGG 1 cut(s) 983
BseRI GAGGAG 1 cut(s) 791
BseSI GKGCMC 1 cut(s) 169
BseXI GCAGC 3 cut(s) 446, 800, 1138
BsgI GTGCAG 1 cut(s) 807
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 50, 1033
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 1 cut(s) 797
BslI CCNNNNNNNGG 3 cut(s) 79, 127, 563
BsmAI GTCTC 1 cut(s) 667
BsmFI GGGAC 1 cut(s) 797
BsnI GGCC 2 cut(s) 50, 1033
Bsp1286I GDGCHC 2 cut(s) 169, 571
Bsp1407I TGTACA 1 cut(s) 841
Bsp143I GATC 4 cut(s) 514, 710, 904, 1056
BspACI CCGC 2 cut(s) 137, 1129
BspANI GGCC 2 cut(s) 50, 1033
BspCNI CTCAG 5 cut(s) 308, 649, 719, 1006, 1125
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 2 cut(s) 337, 568
BspPI GGATC 2 cut(s) 718, 1051
BsrGI TGTACA 1 cut(s) 841
BsrI ACTGG 1 cut(s) 983
BssECI CCNNGG 5 cut(s) 648, 649, 933, 1060, 1061
BssMI GATC 4 cut(s) 514, 710, 904, 1056
BssT1I CCWWGG 1 cut(s) 933
Bst2UI CCWGG 4 cut(s) 476, 650, 976, 1062
Bst4CI ACNGT 4 cut(s) 289, 685, 811, 1039
Bst6I CTCTTC 2 cut(s) 768, 1090
BstAUI TGTACA 1 cut(s) 841
BstDEI CTNAG 5 cut(s) 295, 636, 706, 993, 1133
BstF5I GGATG 3 cut(s) 388, 394, 1013
BstFNI CGCG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 593
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 4 cut(s) 517, 713, 907, 1059
BstMAI GTCTC 1 cut(s) 667
BstMBI GATC 4 cut(s) 514, 710, 904, 1056
BstMWI GCNNNNNNNGC 2 cut(s) 56, 1135
BstNI CCWGG 4 cut(s) 476, 650, 976, 1062
BstSCI CCNGG 4 cut(s) 474, 648, 974, 1060
BstSLI GKGCMC 1 cut(s) 169
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 3 cut(s) 446, 800, 1138
BstV2I GAAGAC 1 cut(s) 106
BstX2I RGATCY 2 cut(s) 710, 1056
BstYI RGATCY 2 cut(s) 710, 1056
Bsu36I CCTNAGG 2 cut(s) 706, 993
BsuI GTATCC 1 cut(s) 999
BsuRI GGCC 2 cut(s) 50, 1033
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 3 cut(s) 388, 394, 1013
BtsI GCAGTG 2 cut(s) 274, 840
BtsIMutI CAGTG 4 cut(s) 274, 514, 840, 990
CaiI CAGNNNCTG 2 cut(s) 839, 1132
CfoI GCGC 1 cut(s) 592
Cfr13I GGNCC 4 cut(s) 49, 103, 749, 1031
CseI GACGC 1 cut(s) 100
Csp6I GTAC 4 cut(s) 199, 285, 822, 842
CviAII CATG 2 cut(s) 533, 572
CviQI GTAC 4 cut(s) 199, 285, 822, 842
DdeI CTNAG 5 cut(s) 295, 636, 706, 993, 1133
DpnI GATC 4 cut(s) 516, 712, 906, 1058
DpnII GATC 4 cut(s) 514, 710, 904, 1056
DraI TTTAAA 1 cut(s) 763
DrdI GACNNNNNNGTC 2 cut(s) 38, 110
DseDI GACNNNNNNGTC 2 cut(s) 38, 110
Eam1104I CTCTTC 2 cut(s) 768, 1090
EarI CTCTTC 2 cut(s) 768, 1090
Eco130I CCWWGG 1 cut(s) 933
Eco24I GRGCYC 1 cut(s) 571
Eco47I GGWCC 2 cut(s) 103, 749
Eco57I CTGAAG 1 cut(s) 297
Eco81I CCTNAGG 2 cut(s) 706, 993
EcoO109I RGGNCCY 1 cut(s) 749
EcoRI GAATTC 1 cut(s) 76
EcoRII CCWGG 4 cut(s) 474, 648, 974, 1060
EcoT14I CCWWGG 1 cut(s) 933
EcoT38I GRGCYC 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 933
FaeI CATG 2 cut(s) 536, 575
FalI AAGNNNNNCTT 2 cut(s) 295, 327
FaqI GGGAC 1 cut(s) 797
FatI CATG 2 cut(s) 532, 571
Fnu4HI GCNGC 4 cut(s) 435, 789, 1127, 1130
FokI GGATG 3 cut(s) 395, 401, 1020
FriOI GRGCYC 1 cut(s) 571
Fsp4HI GCNGC 4 cut(s) 435, 789, 1127, 1130
GlaI GCGC 1 cut(s) 591
GluI GCNGC 4 cut(s) 435, 789, 1127, 1130
GsuI CTGGAG 1 cut(s) 497
HaeII RGCGCY 1 cut(s) 593
HaeIII GGCC 2 cut(s) 50, 1033
HapII CCGG 1 cut(s) 47
HgaI GACGC 1 cut(s) 100
HhaI GCGC 1 cut(s) 592
Hin1II CATG 2 cut(s) 536, 575
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 3 cut(s) 179, 266, 677
HpaII CCGG 1 cut(s) 47
HphI GGTGA 2 cut(s) 245, 686
Hpy166II GTNNAC 3 cut(s) 64, 261, 824
Hpy188I TCNGA 2 cut(s) 103, 298
Hpy188III TCNNGA 8 cut(s) 250, 488, 518, 638, 666, 708, 995, 1013
Hpy8I GTNNAC 3 cut(s) 64, 261, 824
HpyAV CCTTC 1 cut(s) 116
HpyCH4III ACNGT 4 cut(s) 289, 685, 811, 1039
HpyCH4V TGCA 9 cut(s) 86, 279, 633, 788, 807, 833, 853, 880, 1090
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 1135
HpyF3I CTNAG 5 cut(s) 295, 636, 706, 993, 1133
Hsp92II CATG 2 cut(s) 536, 575
HspAI GCGC 1 cut(s) 590
Kzo9I GATC 4 cut(s) 514, 710, 904, 1056
LmnI GCTCC 2 cut(s) 49, 566
Lsp1109I GCAGC 3 cut(s) 446, 800, 1138
LweI GCATC 1 cut(s) 471
MaeIII GTNAC 2 cut(s) 307, 793
MalI GATC 4 cut(s) 516, 712, 906, 1058
MboI GATC 4 cut(s) 514, 710, 904, 1056
MboII GAAGA 5 cut(s) 29, 106, 785, 1058, 1107
MfeI CAATTG 1 cut(s) 1104
MflI RGATCY 2 cut(s) 710, 1056
MhlI GDGCHC 2 cut(s) 169, 571
MluCI AATT 8 cut(s) 76, 154, 359, 528, 605, 1073, 1084, 1104
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 7 cut(s) 31, 62, 388, 715, 769, 1002, 1128
MseI TTAA 2 cut(s) 537, 762
MslI CAYNNNNRTG 1 cut(s) 537
MspA1I CMGCKG 2 cut(s) 791, 1129
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 4 cut(s) 476, 650, 976, 1062
MunI CAATTG 1 cut(s) 1104
MvaI CCWGG 4 cut(s) 476, 650, 976, 1062
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 56, 1135
NdeII GATC 4 cut(s) 514, 710, 904, 1056
NlaIII CATG 2 cut(s) 536, 575
NlaIV GGNNCC 2 cut(s) 337, 568
NmuCI GTSAC 1 cut(s) 307
PasI CCCWGGG 2 cut(s) 649, 1061
PfeI GAWTC 3 cut(s) 179, 266, 677
PkrI GCNGC 4 cut(s) 436, 790, 1128, 1131
PpuMI RGGWCCY 1 cut(s) 749
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 4 cut(s) 474, 648, 974, 1060
PspGI CCWGG 4 cut(s) 474, 648, 974, 1060
PspN4I GGNNCC 2 cut(s) 337, 568
PspPI GGNCC 4 cut(s) 49, 103, 749, 1031
PspPPI RGGWCCY 1 cut(s) 749
PstNI CAGNNNCTG 2 cut(s) 839, 1132
PsuI RGATCY 2 cut(s) 710, 1056
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 4 cut(s) 200, 286, 823, 843
RsaNI GTAC 4 cut(s) 199, 285, 822, 842
RseI CAYNNNNRTG 1 cut(s) 537
SaqAI TTAA 2 cut(s) 537, 762
SatI GCNGC 4 cut(s) 435, 789, 1127, 1130
Sau3AI GATC 4 cut(s) 514, 710, 904, 1056
Sau96I GGNCC 4 cut(s) 49, 103, 749, 1031
ScaI AGTACT 1 cut(s) 286
ScrFI CCNGG 4 cut(s) 476, 650, 976, 1062
SduI GDGCHC 2 cut(s) 169, 571
SetI ASST 8 cut(s) 42, 108, 123, 442, 754, 793, 939, 1119
SfaNI GCATC 1 cut(s) 471
SinI GGWCC 2 cut(s) 103, 749
SmiMI CAYNNNNRTG 1 cut(s) 537
Sse9I AATT 8 cut(s) 76, 154, 359, 528, 605, 1073, 1084, 1104
SsiI CCGC 2 cut(s) 137, 1129
StyD4I CCNGG 4 cut(s) 474, 648, 974, 1060
StyI CCWWGG 1 cut(s) 933
TaaI ACNGT 4 cut(s) 289, 685, 811, 1039
TasI AATT 8 cut(s) 76, 154, 359, 528, 605, 1073, 1084, 1104
TatI WGTACW 3 cut(s) 284, 821, 841
TauI GCSGC 1 cut(s) 1132
TfiI GAWTC 3 cut(s) 179, 266, 677
Tru1I TTAA 2 cut(s) 537, 762
Tru9I TTAA 2 cut(s) 537, 762
TscAI CASTG 4 cut(s) 281, 514, 840, 990
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 3 cut(s) 434, 788, 1126
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 3 cut(s) 161, 521, 1125
TspGWI ACGGA 2 cut(s) 50, 420
TspRI CASTG 4 cut(s) 281, 514, 840, 990
VpaK11BI GGWCC 2 cut(s) 103, 749
XapI RAATTY 6 cut(s) 76, 154, 359, 528, 605, 1084
ZrmI AGTACT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.