FvH4_2g23671

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010766
Physical Location & Seq
Forward (+)
19385159 .. 19386633
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23671.t4

Sequence Viewer

Length: 807 bp
ATGGAGATCACGGCGAGCTCACTGGACGGTTCCGTCATCTTCCACGCCATCAGCGAAATCTCAGGTTTCCTTCTCTTCATGCACCAGCAAATCCCCGCATTGTTGCAGGACATCACCGCGGAATTCGATTCGCGCTGCGAGAACTACAAAGACTTGGAACTTGCTCAGAGAGAAGACCAGGTGAAGCCGGCGCTGCGGAGAAAGCACGCCGGTGAAATGAGGGAAGCCAAGCGAGGGATTCAAAGGTTCCAGAAGCTGATGGCCGGCGTTTCGCATTTCCAGACGGCGCTGAAGACGATCCTCACCGAAACTCCTCGGTTCGAAAAAGCGCTTCTGATTCTCGGAGGGAATCCAATGCGGCCTCAACACGTGTACGAACTGAGCTTCTTCTCCGGAAGAAGTGCTGGTGGTGAAGAAGTTGATTTTGGGAAAAGCAGAGCGGCGGAAGTGCTTTGTAAAAAAGCGATTCGGGGGTTGGTAACGAAGAATGTGGGGTCTGTTTCGTATCCGGGGCCTTTGAAGCTGTTCTTGATGGTTAAGGCGCCCTCTTGTTTCAATCAGCCTCTGCATTTTCTTCCCAAGCGAGATTTTAAATACAGCAACAAGATTGTGCCCATGAGGTTAAGCTTCAAGAGCAAAACAGAGAACCAGAGCATTGATCAGTTTTTGCAAACCGGCAGCTCCAATGGCTTCGCTGAGTCTGATTCAGATGGAGGTGATTTTATATGGTTTCAATGCCGACATGTAATCAAGGGCCTGGCGTTTAACACAGAAGCTGAAGGAGGCATAGCTCAGCATTTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p31comet

Protein Analysis

269

Amino Acids

30.0

Weight (kDa)

8.99

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
AccB1I GGYRCC 1 cut(s) 541
AccBSI CCGCTC 1 cut(s) 440
AccII CGCG 2 cut(s) 119, 133
AccIII TCCGGA 1 cut(s) 392
AciI CCGC 7 cut(s) 96, 117, 119, 196, 358, 440, 443
AclWI GGATC 1 cut(s) 292
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 1 cut(s) 122
AcuI CTGAAG 2 cut(s) 311, 798
AcvI CACGTG 1 cut(s) 370
AcyI GRCGYC 1 cut(s) 542
AfaI GTAC 1 cut(s) 374
AfeI AGCGCT 1 cut(s) 330
AfiI CCNNNNNNNGG 1 cut(s) 234
AflIII ACRYGT 3 cut(s) 367, 369, 742
AgsI TTSAA 5 cut(s) 242, 520, 556, 631, 734
AjnI CCWGG 2 cut(s) 177, 756
AjuI GAANNNNNNNTTGG 2 cut(s) 408, 440
AleI CACNNNNGTG 1 cut(s) 210
AluBI AGCT 8 cut(s) 18, 256, 384, 523, 627, 681, 776, 791
AluI AGCT 8 cut(s) 18, 256, 384, 523, 627, 681, 776, 791
Alw21I GWGCWC 1 cut(s) 20
AlwI GGATC 1 cut(s) 292
AlwNI CAGNNNCTG 3 cut(s) 256, 565, 776
Aor13HI TCCGGA 1 cut(s) 392
Aor51HI AGCGCT 1 cut(s) 330
AoxI GGCC 4 cut(s) 261, 359, 512, 754
ApeKI GCWGC 3 cut(s) 135, 193, 678
ApoI RAATTY 1 cut(s) 122
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 5 cut(s) 135, 193, 289, 331, 544
AspS9I GGNCC 2 cut(s) 512, 754
AsuC2I CCSGG 1 cut(s) 510
AsuHPI GGTGA 6 cut(s) 106, 193, 224, 295, 422, 728
AsuII TTCGAA 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 615
BanI GGYRCC 1 cut(s) 541
BanII GRGCYC 1 cut(s) 20
BbrPI CACGTG 1 cut(s) 370
BbsI GAAGAC 2 cut(s) 180, 299
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 3 cut(s) 122, 180, 690
BccI CCATC 4 cut(s) 56, 253, 526, 704
BceAI ACGGC 2 cut(s) 27, 300
BciT130I CCWGG 2 cut(s) 179, 758
BciVI GTATCC 1 cut(s) 516
BclI TGATCA 1 cut(s) 658
BcnI CCSGG 1 cut(s) 510
BfoI RGCGCY 4 cut(s) 194, 290, 332, 545
BfuI GTATCC 1 cut(s) 516
BisI GCNGC 5 cut(s) 136, 194, 359, 441, 679
BlpI GCTNAGC 1 cut(s) 792
BlsI GCNGC 5 cut(s) 137, 195, 360, 442, 680
Bme1390I CCNGG 3 cut(s) 179, 510, 758
BmgT120I GGNCC 2 cut(s) 512, 754
BmiI GGNNCC 4 cut(s) 31, 248, 513, 543
BmrFI CCNGG 3 cut(s) 179, 510, 758
BpiI GAAGAC 2 cut(s) 180, 299
Bpu1102I GCTNAGC 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 510
BsaAI YACGTR 1 cut(s) 370
BsaHI GRCGYC 1 cut(s) 542
BsaJI CCNNGG 3 cut(s) 117, 314, 509
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 295, 325
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse118I RCCGGY 4 cut(s) 187, 209, 263, 674
Bse1I ACTGG 1 cut(s) 27
BseAI TCCGGA 1 cut(s) 392
BseBI CCWGG 2 cut(s) 179, 758
BseDI CCNNGG 3 cut(s) 117, 314, 509
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMII CTCAG 5 cut(s) 75, 179, 371, 687, 806
BseNI ACTGG 1 cut(s) 27
BseRI GAGGAG 1 cut(s) 303
BseSI GKGCMC 1 cut(s) 615
BseXI GCAGC 3 cut(s) 122, 180, 690
Bsh1236I CGCG 2 cut(s) 119, 133
BshFI GGCC 4 cut(s) 263, 361, 514, 756
BshNI GGYRCC 1 cut(s) 541
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 6 cut(s) 188, 210, 264, 393, 509, 675
BslI CCNNNNNNNGG 1 cut(s) 234
BsnI GGCC 4 cut(s) 263, 361, 514, 756
Bsp119I TTCGAA 1 cut(s) 321
Bsp1286I GDGCHC 2 cut(s) 20, 615
Bsp13I TCCGGA 1 cut(s) 392
Bsp143I GATC 3 cut(s) 6, 297, 658
Bsp1720I GCTNAGC 1 cut(s) 792
BspACI CCGC 7 cut(s) 96, 117, 119, 196, 358, 440, 443
BspANI GGCC 4 cut(s) 263, 361, 514, 756
BspCNI CTCAG 5 cut(s) 74, 178, 372, 688, 805
BspEI TCCGGA 1 cut(s) 392
BspFNI CGCG 2 cut(s) 119, 133
BspLI GGNNCC 4 cut(s) 31, 248, 513, 543
BspPI GGATC 1 cut(s) 292
BspT104I TTCGAA 1 cut(s) 321
BspT107I GGYRCC 1 cut(s) 541
BsrBI CCGCTC 1 cut(s) 440
BsrFI RCCGGY 4 cut(s) 187, 209, 263, 674
BsrI ACTGG 1 cut(s) 27
BssAI RCCGGY 4 cut(s) 187, 209, 263, 674
BssECI CCNNGG 3 cut(s) 117, 314, 509
BssMI GATC 3 cut(s) 6, 297, 658
BssNI GRCGYC 1 cut(s) 542
Bst2UI CCWGG 2 cut(s) 179, 758
Bst4CI ACNGT 1 cut(s) 29
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 370
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 4 cut(s) 16, 189, 207, 265
BstDEI CTNAG 5 cut(s) 61, 165, 380, 696, 792
BstDSI CCRYGG 1 cut(s) 117
BstFNI CGCG 2 cut(s) 119, 133
BstH2I RGCGCY 4 cut(s) 194, 290, 332, 545
BstHHI GCGC 5 cut(s) 135, 193, 289, 331, 544
BstKTI GATC 3 cut(s) 9, 300, 661
BstMBI GATC 3 cut(s) 6, 297, 658
BstMWI GCNNNNNNNGC 5 cut(s) 193, 202, 520, 633, 687
BstNI CCWGG 2 cut(s) 179, 758
BstNSI RCATGY 1 cut(s) 746
BstSCI CCNGG 3 cut(s) 177, 508, 756
BstSLI GKGCMC 1 cut(s) 615
BstUI CGCG 2 cut(s) 119, 133
BstV1I GCAGC 3 cut(s) 122, 180, 690
BstV2I GAAGAC 2 cut(s) 180, 299
BsuI GTATCC 1 cut(s) 516
BsuRI GGCC 4 cut(s) 263, 361, 514, 756
BtgI CCRYGG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 20
Cac8I GCNNGC 4 cut(s) 16, 189, 207, 265
CaiI CAGNNNCTG 3 cut(s) 256, 565, 776
CfoI GCGC 5 cut(s) 135, 193, 289, 331, 544
Cfr10I RCCGGY 4 cut(s) 187, 209, 263, 674
Cfr13I GGNCC 2 cut(s) 512, 754
Cfr42I CCGCGG 1 cut(s) 120
CsiI ACCWGGT 1 cut(s) 177
Csp6I GTAC 1 cut(s) 373
CviAII CATG 3 cut(s) 79, 616, 743
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 5 cut(s) 61, 165, 380, 696, 792
DinI GGCGCC 1 cut(s) 543
DpnI GATC 3 cut(s) 8, 299, 660
DpnII GATC 3 cut(s) 6, 297, 658
DraI TTTAAA 1 cut(s) 592
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
EciI GGCGGA 1 cut(s) 458
Ecl136II GAGCTC 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 20
Eco47III AGCGCT 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 2 cut(s) 311, 798
Eco72I CACGTG 1 cut(s) 370
EcoICRI GAGCTC 1 cut(s) 18
EcoO109I RGGNCCY 2 cut(s) 512, 754
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 177, 756
EcoT38I GRGCYC 1 cut(s) 20
EgeI GGCGCC 1 cut(s) 543
EheI GGCGCC 1 cut(s) 543
FaeI CATG 3 cut(s) 82, 619, 746
FaiI YATR 7 cut(s) 80, 617, 725, 727, 744, 788, 805
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FatI CATG 3 cut(s) 78, 615, 742
FauI CCCGC 1 cut(s) 103
FbaI TGATCA 1 cut(s) 658
Fnu4HI GCNGC 5 cut(s) 136, 194, 359, 441, 679
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 5 cut(s) 136, 194, 359, 441, 679
GlaI GCGC 5 cut(s) 134, 192, 288, 330, 543
GluI GCNGC 5 cut(s) 136, 194, 359, 441, 679
HaeII RGCGCY 4 cut(s) 194, 290, 332, 545
HaeIII GGCC 4 cut(s) 263, 361, 514, 756
HapII CCGG 6 cut(s) 188, 210, 264, 393, 509, 675
HhaI GCGC 5 cut(s) 135, 193, 289, 331, 544
Hin1I GRCGYC 1 cut(s) 542
Hin1II CATG 3 cut(s) 82, 619, 746
Hin6I GCGC 5 cut(s) 133, 191, 287, 329, 542
HinP1I GCGC 5 cut(s) 133, 191, 287, 329, 542
HindIII AAGCTT 1 cut(s) 625
HinfI GANTC 7 cut(s) 128, 238, 337, 349, 466, 698, 704
HpaII CCGG 6 cut(s) 188, 210, 264, 393, 509, 675
HphI GGTGA 6 cut(s) 106, 193, 224, 295, 422, 728
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 5 cut(s) 168, 336, 344, 703, 709
Hpy188III TCNNGA 5 cut(s) 250, 280, 393, 529, 631
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 2 cut(s) 80, 773
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 4 cut(s) 82, 106, 568, 670
HpyF10VI GCNNNNNNNGC 5 cut(s) 193, 202, 520, 633, 687
HpyF3I CTNAG 5 cut(s) 61, 165, 380, 696, 792
HpySE526I ACGT 1 cut(s) 369
Hsp92I GRCGYC 1 cut(s) 542
Hsp92II CATG 3 cut(s) 82, 619, 746
HspAI GCGC 5 cut(s) 133, 191, 287, 329, 542
KasI GGCGCC 1 cut(s) 541
Kpn2I TCCGGA 1 cut(s) 392
KroI GCCGGC 2 cut(s) 187, 263
KroNI GCCGGC 2 cut(s) 189, 265
Ksp22I TGATCA 1 cut(s) 658
KspI CCGCGG 1 cut(s) 120
Kzo9I GATC 3 cut(s) 6, 297, 658
LmnI GCTCC 1 cut(s) 686
Lsp1109I GCAGC 3 cut(s) 122, 180, 690
MabI ACCWGGT 1 cut(s) 177
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 1 cut(s) 478
MalI GATC 3 cut(s) 8, 299, 660
MbiI CCGCTC 1 cut(s) 440
MboI GATC 3 cut(s) 6, 297, 658
MboII GAAGA 9 cut(s) 31, 67, 185, 304, 379, 408, 425, 496, 566
MhlI GDGCHC 2 cut(s) 20, 615
MluCI AATT 1 cut(s) 122
Mly113I GGCGCC 1 cut(s) 542
MlyI GAGTC 1 cut(s) 707
MroI TCCGGA 1 cut(s) 392
MroNI GCCGGC 2 cut(s) 187, 263
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 4 cut(s) 537, 591, 623, 765
MslI CAYNNNNRTG 1 cut(s) 210
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 6 cut(s) 188, 210, 264, 393, 509, 675
MspR9I CCNGG 3 cut(s) 179, 510, 758
MvaI CCWGG 2 cut(s) 179, 758
MvnI CGCG 2 cut(s) 119, 133
MwoI GCNNNNNNNGC 5 cut(s) 193, 202, 520, 633, 687
NaeI GCCGGC 2 cut(s) 189, 265
NarI GGCGCC 1 cut(s) 542
NciI CCSGG 1 cut(s) 510
NdeII GATC 3 cut(s) 6, 297, 658
NgoMIV GCCGGC 2 cut(s) 187, 263
NlaIII CATG 3 cut(s) 82, 619, 746
NlaIV GGNNCC 4 cut(s) 31, 248, 513, 543
NspI RCATGY 1 cut(s) 746
NspV TTCGAA 1 cut(s) 321
OliI CACNNNNGTG 1 cut(s) 210
PciI ACATGT 1 cut(s) 742
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PdiI GCCGGC 2 cut(s) 189, 265
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 6 cut(s) 128, 238, 337, 349, 466, 704
PkrI GCNGC 5 cut(s) 137, 195, 360, 442, 680
PleI GAGTC 1 cut(s) 706
PluTI GGCGCC 1 cut(s) 545
PmaCI CACGTG 1 cut(s) 370
PmlI CACGTG 1 cut(s) 370
PpsI GAGTC 1 cut(s) 706
Ppu21I YACGTR 1 cut(s) 370
PscI ACATGT 1 cut(s) 742
Psp124BI GAGCTC 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 177, 756
PspCI CACGTG 1 cut(s) 370
PspGI CCWGG 2 cut(s) 177, 756
PspN4I GGNNCC 4 cut(s) 31, 248, 513, 543
PspPI GGNCC 2 cut(s) 512, 754
PstNI CAGNNNCTG 3 cut(s) 256, 565, 776
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
RseI CAYNNNNRTG 1 cut(s) 210
SacI GAGCTC 1 cut(s) 20
SacII CCGCGG 1 cut(s) 120
SaqAI TTAA 4 cut(s) 537, 591, 623, 765
SatI GCNGC 5 cut(s) 136, 194, 359, 441, 679
Sau3AI GATC 3 cut(s) 6, 297, 658
Sau96I GGNCC 2 cut(s) 512, 754
SchI GAGTC 1 cut(s) 707
ScrFI CCNGG 3 cut(s) 179, 510, 758
SduI GDGCHC 2 cut(s) 20, 615
SexAI ACCWGGT 1 cut(s) 177
SfoI GGCGCC 1 cut(s) 543
Sfr303I CCGCGG 1 cut(s) 120
SfuI TTCGAA 1 cut(s) 321
SgrAI CRCCGGYG 1 cut(s) 209
SgrBI CCGCGG 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 210
Sse9I AATT 1 cut(s) 122
SsiI CCGC 7 cut(s) 96, 117, 119, 196, 358, 440, 443
SspDI GGCGCC 1 cut(s) 541
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 3 cut(s) 177, 508, 756
TaaI ACNGT 1 cut(s) 29
TaiI ACGT 1 cut(s) 372
TaqI TCGA 2 cut(s) 126, 321
TasI AATT 1 cut(s) 122
TauI GCSGC 2 cut(s) 361, 443
TfiI GAWTC 6 cut(s) 128, 238, 337, 349, 466, 704
Tru1I TTAA 4 cut(s) 537, 591, 623, 765
Tru9I TTAA 4 cut(s) 537, 591, 623, 765
TscAI CASTG 1 cut(s) 27
TseI GCWGC 3 cut(s) 135, 193, 678
TspDTI ATGAA 2 cut(s) 67, 792
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 1 cut(s) 27
XapI RAATTY 1 cut(s) 122
XceI RCATGY 1 cut(s) 746
XmnI GAANNNNTTC 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.